• NCBI MSA Viewer 1.25.3
  • Help
  • Release Notes

NCBI Multiple Sequence Alignment Viewer

The NCBI Multiple Sequence Alignment Viewer (MSA) is a graphical display for nucleotide and protein sequence alignments.

Review documentation or watch a video tutorial.

To see your own alignment, your data

Sample MSA Viewer

Examples of various alignment styles:

  • Protein alignment with no anchor set
  • Protein alignment, anchor set to ACI28628
  • Protein alignment using FASTA format from the MUSCLE program
  • Nucleotide alignment from Blast RID with query set as anchor; primate genomic, mRNA, and BAC sequences
  • Protein alignment from Blast RID, metazoan proteins belonging to the LIN37 protein family
  • Alignment of prion protein gene sequences from S. cerevisiae PopSet
  • Polyprotein alignment with anchor, Dengue virus 2
  • Genomic alignment with consensus, Dengue virus 1
  • Alignment of nucleocapsid coding region, Influenza A virus (nonsynonymous substitutions coloring)
  • Alignment of polymerase PB1 coding region, Influenza A virus (nonsynonymous substitutions coloring)

You can also embed Multiple Sequence Alignment Viewer on your own page. Following are demo pages with embedded MSA Viewer

  • Static embedding
  • Dynamic embedding
  • Interaction with SV on the same page
  • MSAV event handling
  • Different coloring options
  • Alignment Coordinate transformation
  • Consensus parameters
  • Sorting parameters
  • Columns re-arranging