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<li><p><a class="reference internal" href="#web-service-interface" id="id2">Web service interface</a></p></li>
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<li><p><a class="reference internal" href="#rest" id="id3">REST</a></p></li>
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<li><p><a class="reference internal" href="#c-toolkit-implementation" id="id4">C++ Toolkit implementation</a></p></li>
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<section id="overview">
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<span id="developerinfo"></span><h1><a class="toc-backref" href="#id1" role="doc-backlink">Overview</a><a class="headerlink" href="#overview" title="Link to this heading">¶</a></h1>
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<p>BLAST is public domain software.</p>
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<p>Public-domain software is software that has been placed in the public domain, in other words, software for which there is absolutely no ownership such as
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copyright, trademark, or patent. Software in the public domain can be modified, distributed, or sold even without any attribution by anyone;
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this is unlike the common case of software under exclusive copyright, where licenses grant limited usage rights.</p>
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</section>
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<section id="web-service-interface">
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<h1><a class="toc-backref" href="#id2" role="doc-backlink">Web service interface</a><a class="headerlink" href="#web-service-interface" title="Link to this heading">¶</a></h1>
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<p>The NCBI supports RESTful interface to programmatically submit BLAST searches. Users of the service at the NCBI should respect the usage guidelines below.</p>
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</section>
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<section id="rest">
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<h1><a class="toc-backref" href="#id3" role="doc-backlink">REST</a><a class="headerlink" href="#rest" title="Link to this heading">¶</a></h1>
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<p>A RESTful interface is supported for submission of BLAST searches via an HTTP based interface.
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Searches are submitted to the NCBI servers. <a class="reference internal" href="urlapi.html#urlapi"><span class="std std-ref">Documentation</span></a> and <a class="reference external" href="https://blast.ncbi.nlm.nih.gov/docs/web_blast.pl">sample perl code</a> are available. This is a public resource, so usage limitations apply. Projects
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that involve a large number of BLAST searches should use the RESTful interface at a cloud provider or stand-alone BLAST.</p>
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<section id="usage-guidelines">
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<h2>Usage Guidelines<a class="headerlink" href="#usage-guidelines" title="Link to this heading">¶</a></h2>
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<p>The NCBI BLAST servers are a shared resource. We give priority to interactive users. In order to ensure availability of the service to the entire community, we may limit searches
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for some high volume users. Interactive users of the NCBI webpages through a web browser should not encounter problems. We will move searches of users who submit more than 100 searches in a
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24 hour period to a slower queue, or, in extreme cases, will block the requests. To avoid problems, API users should comply with the following guidelines:</p>
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<ul class="simple">
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<li><p>Do not contact the server more often than once every 10 seconds.</p></li>
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<li><p>Do not poll for any single RID more often than once a minute.</p></li>
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<li><p>Use the URL parameter email and tool, so that the NCBI can contact you if there is a problem.</p></li>
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<li><p>Run scripts weekends or between 9 pm and 5 am Eastern time on weekdays if more than 50 searches will be submitted.</p></li>
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</ul>
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<p>BLAST often runs more efficiently if multiple queries are sent as one search rather than if each query is sent as an individual search. This is especially true for blastn,
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megablast, and tblastn. If your queries are short (less than a few hundred bases) we suggest you merge them into one search of up to 1,000 bases</p>
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<p>The NCBI servers are a shared resource and not intended for projects that involve a large number of BLAST searches.
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We provide the <a class="reference external" href="https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastDocs&DOC_TYPE=Download">Stand-alone BLAST+ binaries</a>,
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||
<a class="reference external" href="https://hub.docker.com/r/ncbi/blast">Docker Image</a> and <a class="reference external" href="https://blast.ncbi.nlm.nih.gov/doc/elastic-blast/">Elastic BLAST</a> for these purposes.</p>
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</section>
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</section>
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<section id="c-toolkit-implementation">
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<h1><a class="toc-backref" href="#id4" role="doc-backlink">C++ Toolkit implementation</a><a class="headerlink" href="#c-toolkit-implementation" title="Link to this heading">¶</a></h1>
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<p>The currently-maintained implementation of BLAST is part of the <a class="reference external" href="https://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/">NCBI C++ Toolkit</a>.</p>
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<p>The algorithm core is written in C and a <a class="reference external" href="https://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/doxyhtml/group__AlgoBlast.html">documented C++ API</a> is available.</p>
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<p>The source code may be <a class="reference external" href="https://www.ncbi.nlm.nih.gov/toolkit/doc/book/ch_getcode_svn/">downloaded by FTP</a> and
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<a class="reference external" href="https://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/lxr/source/src/algo/blast/">browsed with LXR</a>.</p>
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