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<input type="email" aria-label="Recipient Email Address" placeholder="email@example.com" id="email-to" class="email-to" pattern="^[a-zA-Z0-9_.+-]+@[a-zA-Z0-9-]+\.[a-zA-Z0-9-.]+$" maxlength="100" required>
</div>
<div class="action-panel-control-wrap">
<label for="email-from" class="action-panel-label">
From:
</label>
<input type="email" aria-label="Sender Email Address" placeholder="email@example.com" id="email-from" class="email-from" pattern="^[a-zA-Z0-9_.+-]+@[a-zA-Z0-9-]+\.[a-zA-Z0-9-.]+$" maxlength="256">
</div>
<div class="action-panel-control-wrap">
<label for="email-citation-format" class="action-panel-label">
Format:
</label>
<select id="email-citation-format" name="citation-format" class="action-panel-selector email-citation-format">
<option selected="selected" value="summary">Summary</option>
<option value="summary-text">Summary (text)</option>
<option value="abstract">Abstract</option>
<option value="abstract-text">Abstract (text)</option>
</select>
</div>
<div class="include-supplemental-container">
<input type="checkbox" aria-label="Include MeSH and other data" name="include-supplemental" id="email-include-supplemental" class="email-include-supplemental">
<label for="email-include-supplemental" class="email-include-supplemental-label">MeSH and other data</label>
</div>
<div class="form-field recaptcha ">
<div class="g-recaptcha" id="id-recaptcha" data-sitekey="6LfsWHMdAAAAAClKbtOpjQ2pMjgsGxvv7NdZW9uI"></div>
</div>
<div id="captcha-error-message" class="usa-input-error-message captcha-validation-message" role="alert"></div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Sending..."
data-ga-category="save_share"
data-ga-action="email"
data-ga-label="send">
Send email
</button>
<button class="action-panel-cancel"
aria-label="Close 'Email citations' panel"
ref="linksrc=close_email_panel"
aria-controls="email-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="email"
data-ga-label="cancel">
Cancel
</button>
</div>
<input type="hidden" name="email-search-details" value="" />
<input type="hidden" name="email-search-details-hash" value="0e42663a6c3bd85498fcb88798998fed7bfdc45d457db35281e41afe13cc0524" />
</form>
</div>
</div>
<div id="collections-action-panel"
class="collections-action-panel action-panel in-progress-dots-panel"
aria-hidden="true"
data-collections-open-panel-enabled="false"
data-collections-open-panel-url-hash="#open-collections-panel">
<div class="inner-wrap">
<h3 class="action-panel-heading">
Add to Collections
</h3>
<form id="collections-action-panel-form"
class="collections-action-panel-form action-panel-content action-form action-panel-smaller-selectors"
data-existing-collections-url="/list-existing-collections/"
data-add-to-existing-collection-url="/add-to-existing-collection/"
data-create-and-add-to-new-collection-url="/create-and-add-to-new-collection/"
data-get-article-ids-by-search-url="/get-article-ids-by-search/"
data-myncbi-max-collection-name-length="100"
data-add-to-collection-max-amount="1000"
data-collections-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/">
<input type="hidden" name="csrfmiddlewaretoken" value="zZtwue6Gze7bVDo7kOs9jtqSog6gFPw5oxtJ8ECYegMXgH2dbbS6vrV4VKbCM32d">
<div class="choice-group" role="radiogroup">
<ul class="radio-group-items">
<li>
<input type="radio"
id="collections-action-panel-new"
class="collections-new"
name="collections"
value="new"
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="collections_radio_new">
<label for="collections-action-panel-new">Create a new collection</label>
</li>
<li>
<input type="radio"
id="collections-action-panel-existing"
class="collections-existing"
name="collections"
value="existing"
checked="true"
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="collections_radio_existing">
<label for="collections-action-panel-existing">Add to an existing collection</label>
</li>
</ul>
</div>
<div class="controls-wrapper">
<div class="action-panel-control-wrap new-collections-controls">
<label for="collections-action-panel-add-to-new" class="action-panel-label required-field-asterisk">
Name your collection:
</label>
<input
type="text"
name="add-to-new-collection"
id="collections-action-panel-add-to-new"
class="collections-action-add-to-new"
pattern="[^&quot;&amp;=&lt;&gt;\/]*" title="The following characters are not allowed in the Name field: &quot;&amp;=&lt;&gt;/"
maxlength="100"
data-ga-category="save_share"
data-ga-action="create_collection"
data-ga-label="non_favorties_collection">
<div class="collections-new-name-too-long usa-input-error-message selection-validation-message">
Name must be less than 100 characters
</div>
</div>
<div class="action-panel-control-wrap existing-collections-controls">
<label for="collections-action-panel-add-to-existing" class="action-panel-label">
Choose a collection:
</label>
<select id="collections-action-panel-add-to-existing"
class="action-panel-selector collections-action-add-to-existing"
data-ga-category="save_share"
data-ga-action="select_collection"
data-ga-label="($('#collections-action-add-to-existing').val() === 'Favorites') ? 'Favorites' : 'non_favorites_collection'">
</select>
<div class="collections-retry-load-on-error usa-input-error-message selection-validation-message">
Unable to load your collection due to an error<br>
<a href="#">Please try again</a>
</div>
</div>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Adding..."
data-pinger-ignore
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="add">
Add
</button>
<button class="action-panel-cancel"
aria-label="Close 'Add to Collections' panel"
ref="linksrc=close_collections_panel"
aria-controls="collections-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="bibliography-action-panel"
class="bibliography-action-panel action-panel in-progress-dots-panel"
aria-hidden="true"
data-bibliography-open-panel-enabled="false"
data-bibliography-open-panel-url-hash="#open-bibliography-panel">
<div class="inner-wrap">
<h3 class="action-panel-heading">
Add to My Bibliography
</h3>
<form id="bibliography-action-panel-form"
class="bibliography-action-panel-form action-panel-content action-form action-panel-smaller-selectors"
data-add-to-bibliography-max-amount="100"
data-add-to-bibliography-batch-size="10"
data-bibliography-delegates-url="/list-bibliography-delegates/"
data-add-to-bibliography-url="/add-to-bibliography/"
data-get-article-ids-by-search-url="/get-article-ids-by-search/"
data-mybib-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/mybibliography/">
<input type="hidden" name="csrfmiddlewaretoken" value="zZtwue6Gze7bVDo7kOs9jtqSog6gFPw5oxtJ8ECYegMXgH2dbbS6vrV4VKbCM32d">
<div class="action-panel-control-wrap bibliographies-controls">
<div class="choice-group">
<ul class="bibliographies-action-add radio-group-items">
<li>
<input name="bibliography" id="my-bibliography" class="my-bibliography" type="radio" checked/>
<label for="my-bibliography">My Bibliography</label>
</li>
</ul>
</div>
</div>
<div class="bibliographies-retry-load-on-error usa-input-error-message selection-validation-message">
Unable to load your delegates due to an error<br>
<a href="#">Please try again</a>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Adding..."
data-pinger-ignore>
Add
</button>
<button class="action-panel-cancel"
aria-label="Close 'Add to bibliography' panel"
ref="linksrc=close_bibliography_panel"
aria-controls="bibliography-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="mybib"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="saved-search-action-panel" class="saved-search-action-panel action-panel " aria-hidden="true"
data-saved-search-open-panel-enabled="false"
data-saved-search-open-panel-url-hash="#open-saved-search-panel">
<div class="inner-wrap">
<h2 class="action-panel-heading">
Your saved search
</h2>
<form id="saved-search-action-panel-form"
class="saved-search-action-panel-form action-panel-content action-form"
data-create-saved-search-url="/create-saved-search/"
data-try-search-terms-url="/try-search-term/"
data-saved-search-root-url="https://www.ncbi.nlm.nih.gov/myncbi/searches/">
<input type="hidden" name="csrfmiddlewaretoken" value="zZtwue6Gze7bVDo7kOs9jtqSog6gFPw5oxtJ8ECYegMXgH2dbbS6vrV4VKbCM32d">
<div class="action-panel-control-wrap">
<label for="saved-search-name" class="action-panel-label saved-search-name-label required-field-asterisk">
Name of saved search:
</label>
<input maxlength="200"
type="text"
name="saved-search-name"
id="saved-search-name"
class="saved-search-name"
value=""
required
pattern="[^&quot;&amp;=&lt;&gt;\/]*" title="The following characters are not allowed in the Name field: &quot;&amp;=&lt;&gt;/">
</div>
<div class="action-panel-control-wrap">
<label for="saved-search-term" class="action-panel-label required-field-asterisk">
Search terms:
</label>
<textarea name="saved-search-term" id="saved-search-term" class="saved-search-term" required></textarea>
</div>
<div class="test-search-term-wrap">
<a href="#" class="try-search-term">Test search terms</a>
</div>
<div class="choice-group action-panel-extra-margin-top">
<span class="action-panel-label" id="fieldset-label">
Would you like email updates of new search results?
</span>
<fieldset id="saved-search-alert" aria-describedby="fieldset-label">
<legend class="usa-sr-only">Saved Search Alert Radio Buttons</legend>
<ul class="radio-group-items">
<li>
<input type="radio" id="saved-search-alert-yes" class="saved-search-alert-yes" name="saved-search-alert" value="yes" checked>
<label for="saved-search-alert-yes" class="action-panel-label">Yes</label>
</li>
<li>
<input aria-label="No radio input" type="radio" id="saved-search-alert-no" class="saved-search-alert-no" name="saved-search-alert" value="no">
<label for="saved-search-alert-no" class="action-panel-label">No</label>
</li>
</ul>
</fieldset>
</div>
<div class="alert-schedule-wrap">
<div class="action-panel-control-wrap">
<label class="action-panel-label">
Email:
</label>
<span aria-label="Email address" id="saved-search-email" class="action-panel-label"><span class="action-panel-label-bold"></span> (<a class="myncbi-account-settings" href="https://www.ncbi.nlm.nih.gov/account/settings/">change</a>)</span>
</div>
<div class="action-panel-control-wrap action-panel-extra-margin-top">
<label for="saved-search-frequency" class="action-panel-label">
Frequency:
</label>
<select id="saved-search-frequency" class="no-border-panel-selector saved-search-frequency">
<option value="monthly">Monthly</option>
<option value="weekly">Weekly</option>
<option value="daily">Daily</option>
</select>
</div>
<div class="action-panel-control-wrap saved-search-monthly-additional">
<label for="saved-search-monthly-on-day" class="action-panel-label">
Which day?
</label>
<select id="saved-search-monthly-on-day" class="no-border-panel-selector">
<option value="Sunday">The first Sunday</option>
<option value="Monday">The first Monday</option>
<option value="Tuesday">The first Tuesday</option>
<option value="Wednesday">The first Wednesday</option>
<option value="Thursday">The first Thursday</option>
<option value="Friday">The first Friday</option>
<option value="Saturday">The first Saturday</option>
<option value="day">The first day</option>
<option value="weekday">The first weekday</option>
</select>
</div>
<div class="action-panel-control-wrap saved-search-weekly-additional">
<label for="saved-search-weekly-on-day" class="action-panel-label">
Which day?
</label>
<select id="saved-search-weekly-on-day" class="no-border-panel-selector saved-search-weekly-on-day">
<option value="Sunday">Sunday</option>
<option value="Monday">Monday</option>
<option value="Tuesday">Tuesday</option>
<option value="Wednesday">Wednesday</option>
<option value="Thursday">Thursday</option>
<option value="Friday">Friday</option>
<option value="Saturday">Saturday</option>
</select>
</div>
<div class="action-panel-control-wrap">
<label for="saved-search-report" class="action-panel-label">
Report format:
</label>
<select id="saved-search-report" class="no-border-panel-selector saved-search-report">
<option value="DocSum">Summary</option>
<option value="DocSumText">Summary (text)</option>
<option value="Abstract">Abstract</option>
<option value="AbstractText">Abstract (text)</option>
<option value="MEDLINE">PubMed</option>
</select>
</div>
<div class="action-panel-control-wrap">
<label for="saved-search-amount" class="action-panel-label">
Send at most:
</label>
<select id="saved-search-amount" class="no-border-panel-selector saved-search-amount">
<option value="1">1 item</option>
<option value="5" selected>5 items</option>
<option value="10">10 items</option>
<option value="20">20 items</option>
<option value="50">50 items</option>
<option value="100">100 items</option>
<option value="200">200 items</option>
</select>
</div>
<div>
<input type="checkbox" id="saved-search-send-if-no-result" class="saved-search-send-if-no-result" name="saved-search-send-if-no-result">
<label for="saved-search-send-if-no-result" class="action-panel-label smaller-checkbox">
Send even when there aren't any new results
</label>
</div>
<div class="action-panel-control-wrap option-text-in-email-wrap">
<label for="saved-search-email-text" class="action-panel-label">
Optional text in email:
</label>
<textarea name="saved-search-email-text"
id="saved-search-email-text"
class="saved-search-email-text"></textarea>
</div>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Saving..."
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="save">
Save
</button>
<button class="action-panel-cancel"
aria-label="Close 'Your saved search' panel"
ref="linksrc=close_saved_search_panel"
aria-controls="saved-search-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="citation-manager-action-panel" class="citation-manager-action-panel action-panel" aria-hidden="true">
<div class="inner-wrap">
<h2 class="action-panel-heading">
Create a file for external citation management software
</h2>
<form id="citation-manager-action-panel-form"
class="action-panel-content action-form"
action="/results-export-ids/"
data-by-search-action="/results-export-search-data/"
data-by-ids-action="/results-export-ids/"
method="post"
data-by-search-method="post"
data-by-ids-method="post">
<input type="hidden" name="csrfmiddlewaretoken" value="zZtwue6Gze7bVDo7kOs9jtqSog6gFPw5oxtJ8ECYegMXgH2dbbS6vrV4VKbCM32d">
<input name="results-format" type="hidden" value="pubmed"/>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Sending..."
data-ga-category="save_share"
data-ga-action="citation_manager"
data-ga-label="save">
Create file
</button>
<button class="action-panel-cancel"
aria-label="Close 'Send citations to citation manager' panel"
ref="linksrc=close_citation_manager_panel"
aria-controls="citation-manager-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="citation_manager"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="rss-action-panel" class="rss-action-panel action-panel " aria-hidden="true">
<div class="inner-wrap">
<h2 class="action-panel-heading">
Your RSS Feed
</h2>
<form id="rss-action-panel-form"
class="rss-action-panel-form action-panel-content action-form"
data-create-rss-feed-url="/create-rss-feed-url/"
data-search-form-term-value="">
<input type="hidden" name="csrfmiddlewaretoken" value="zZtwue6Gze7bVDo7kOs9jtqSog6gFPw5oxtJ8ECYegMXgH2dbbS6vrV4VKbCM32d">
<div class="action-panel-control-wrap">
<label for="rss-name" class="action-panel-label required-field-asterisk">
Name of RSS Feed:
</label>
<input maxlength="200"
placeholder="Name"
type="text"
name="rss-name"
id="rss-name"
class="rss-name"
value=''
required
pattern="[^&quot;&amp;=&lt;&gt;\/]*" title="The following characters are not allowed in the Name field: &quot;&amp;=&lt;&gt;/">
</div>
<div class="rss-limit-wrap">
<div class="action-panel-control-wrap action-panel-extra-margin-top">
<label for="rss-limit" class="action-panel-label">
Number of items displayed:
</label>
<select id="rss-limit" class="no-border-panel-selector rss-limit">
<option value="5">5</option>
<option value="10">10</option>
<option value="15" selected="selected">15</option>
<option value="20">20</option>
<option value="50">50</option>
<option value="100">100</option>
</select>
</div>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Creating..."
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="save">
Create RSS
</button>
<button class="action-panel-cancel"
aria-label="Close 'Your RSS' panel"
ref="linksrc=close_rss_panel"
aria-controls="rss-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="cancel">
Cancel
</button>
</div>
<div class="action-panel-control-wrap rss-link-copy-wrap">
<label for="rss-link" class="usa-sr-only">RSS Link</label>
<input placeholder="Your RSS Feed Link" type="text" name="rss-link" id="rss-link" class="rss-link" title="RSS Link">
<button
type="button"
disabled
class="rss-link-copy-button disabled"
data-ga-category="save_share"
data-ga-action="rss"
data-ga-label="copy">
Copy
</button>
</div>
</form>
</div>
</div>
</div>
</div>
<div class="article-page" id="article-page" data-article-pmid="11416127">
<aside class="page-sidebar">
<div class="inner-wrap">
<div class="full-text-links">
<div class="full-view">
<h3 class="title">
Full text links
</h3>
<div class="full-text-links-list">
<a class="link-item
dialog-focus"
href="https://www.tandfonline.com/doi/10.1128/MCB.21.14.4470-4481.2001?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub 0pubmed"
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ref="linksrc=fulltextorjournal_fulltext&amp;is_pmc=False&amp;PrId=4487&amp;itool=Abstract-def&amp;log$=linkouticon&amp;uid=11416127&amp;db=pubmed&amp;nlmid=8109087"
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Atypon
</span></a><a class="link-item
pmc
"
href="https://pmc.ncbi.nlm.nih.gov/articles/pmid/11416127/"
target="_blank"
rel="noopener"
ref="linksrc=fulltextorjournal_fulltext&amp;is_pmc=True&amp;PrId=3494&amp;itool=Abstract-def&amp;log$=linkouticon&amp;uid=11416127&amp;db=pubmed&amp;nlmid=8109087"
title="Free full text at PubMed Central"
data-ga-category="full_text"
data-ga-action="PMC"
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Free PMC article
</span></a>
</div>
</div>
<div class="short-view">
<a href="#" class="full-text-links-button full-text-links-dialog-trigger">
Full text links
</a>
</div>
</div>
<div class="actions-buttons sidebar"><h3 class="title">Actions</h3><div class="inner-wrap"><button class="citation-button citation-dialog-trigger"
aria-label="Open dialog with citation text in different styles"
data-ga-category="save_share"
data-ga-action="cite"
data-ga-label="open"
data-all-citations-url="/11416127/citations/"
data-citation-style="nlm"
data-pubmed-format-link="/11416127/export/"><span class="button-label">Cite</span></button><link type="text/css" href="ncbi-overlay-block/src/overlay-block.css"><div class="collections-button-container" data-article-id="11416127" data-article-db="pubmed"><button class="collections-button collections-dialog-trigger"
aria-label="Save article in MyNCBI collections."
data-ga-category="collections_button"
data-ga-action="click"
data-ga-label="collections_button"
data-collections-open-dialog-enabled="false"
data-collections-open-dialog-url="https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F11416127%2F%23open-collections-dialog"
data-in-collections="false"><span class="button-label">Collections</span></button><div class="overlay" role="dialog"><div id="collections-action-dialog"
class="dialog collections-dialog"
aria-hidden="true"><div class="title">Add to Collections</div><div class="collections-action-panel action-panel"><form id="collections-action-dialog-form"
class="collections-action-panel-form action-panel-content action-form action-panel-smaller-selectors"
data-existing-collections-url="/list-existing-collections/"
data-add-to-existing-collection-url="/add-to-existing-collection/"
data-create-and-add-to-new-collection-url="/create-and-add-to-new-collection/"
data-myncbi-max-collection-name-length="100"
data-collections-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/"><input type="hidden" name="csrfmiddlewaretoken" value="zZtwue6Gze7bVDo7kOs9jtqSog6gFPw5oxtJ8ECYegMXgH2dbbS6vrV4VKbCM32d"><div class="choice-group" role="radiogroup"><ul class="radio-group-items"><li><input type="radio"
id="collections-action-dialog-new"
class="collections-new"
name="collections"
value="new"
data-ga-category="collections_button"
data-ga-action="click"
data-ga-label="collections_radio_new"><label for="collections-action-dialog-new">Create a new collection</label></li><li><input type="radio"
id="collections-action-dialog-existing"
class="collections-existing"
name="collections"
value="existing"
checked="true"
data-ga-category="collections_button"
data-ga-action="click"
data-ga-label="collections_radio_existing"><label for="collections-action-dialog-existing">Add to an existing collection</label></li></ul></div><div class="controls-wrapper"><div class="action-panel-control-wrap new-collections-controls"><label for="collections-action-dialog-add-to-new" class="action-panel-label required-field-asterisk">
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<h2 class="title">
Abstract
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id="eng-abstract">
<p>
The group IV cytosolic phospholipase A(2) (cPLA(2)) has been localized to the nucleus (M. R. Sierra-Honigmann, J. R. Bradley, and J. S. Pober, Lab. Investig. 74:684-695, 1996) and is known to translocate from the cytosolic compartment to the nuclear membrane (S. Glover, M. S. de Carvalho, T. Bayburt, M. Jonas, E. Chi, C. C. Leslie, and M. H. Gelb, J. Biol. Chem. 270:15359-15367, 1995; A. R. Schievella, M. K. Regier, W. L. Smith, and L. L. Lin, J. Biol. Chem. 270:30749-30754, 1995). We hypothesized that nuclear proteins interact with cPLA(2) and participate in the functional effects of this translocation. We have identified a nuclear protein, cPLA(2)-interacting protein (PLIP), a splice variant of human Tip60, which interacts with the amino terminal region of cPLA(2). Like Tip60, PLIP cDNA includes the MYST domain containing a C2HC zinc finger and well-conserved similarities to acetyltransferases. Both PLIP and Tip60 coimmunoprecipitate and colocalize with cPLA(2) within the nuclei of transfected COS cells. A polyclonal antibody raised to PLIP recognizes both PLIP and Tip60. Endogenous Tip60 and/or PLIP in rat mesangial cells is localized to the nucleus in response to serum deprivation. Nuclear localization coincides temporally with apoptosis. PLIP expression, mediated by adenoviral gene transfer, potentiates serum deprivation-induced prostaglandin E(2) (PGE(2)) production and apoptosis in mouse mesangial cells from cPLA(2)(+/+) mice but not in mesangial cells derived from cPLA(2)(-/-) mice. Thus PLIP, a splice variant of Tip60, interacts with cPLA(2) and potentiates cPLA(2)-mediated PGE(2) production and apoptosis.
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<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="0"
data-label-slug="fig-1">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/def70b1b3e4a/mb1410490001.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-0"
role="button"
data-image-width="666"
data-image-height="743"
data-image-alt="FIG. 1"
data-pmc-id="PMC87107"
data-figure-id="F1">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-0"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/30fbaaacf245/mb1410490001.gif"
alt="FIG. 1" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="666">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="743">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 1 </p>
</strong>
<div class="figure-caption-contents"><p> Nucleotide and predicted amino acid… </p></div>
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</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 1 </p>
</strong>
<div class="figure-caption-contents"><p> Nucleotide and predicted amino acid sequence of PLIP. The MYST domain is boxed,… </p></div>
</div>
<figcaption id="figure-caption-0" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 1
</strong>
<div class="figure-caption-contents">Nucleotide and predicted amino acid sequence of PLIP. The MYST domain is boxed, and within the box the acetyltransferase domain is double underlined. A C2HC domain, also within the boxed MYST domain, is single underlined. A potential nuclear localization sequence at the carboxy terminus is double underlined. Potential ERK1/2 kinase phosphorylation sites (PGSP and PATP) are single underlined. The potential CDK phosphorylation site is triple underlined.</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="1"
data-label-slug="fig-2">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/e1f72964daf7/mb1410490002.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-1"
role="button"
data-image-width="580"
data-image-height="353"
data-image-alt="FIG. 2"
data-pmc-id="PMC87107"
data-figure-id="F2">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-1"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/21e77f92e7bd/mb1410490002.gif"
alt="FIG. 2" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="580">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="353">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 2 </p>
</strong>
<div class="figure-caption-contents"><p> Structure of PLIP locus. Partial… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 2 </p>
</strong>
<div class="figure-caption-contents"><p> Structure of PLIP locus. Partial genomic structure is represented by exons consecutively numbered… </p></div>
</div>
<figcaption id="figure-caption-1" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 2
</strong>
<div class="figure-caption-contents">Structure of PLIP locus. Partial genomic structure is represented by exons consecutively numbered 1 to 14. Tip60, but not PLIP cDNA, contains exon 5. Both Tip60 and PLIP contain the MYST domain.</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="2"
data-label-slug="fig-3">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/cd07eb5beda2/mb1410490003.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-2"
role="button"
data-image-width="552"
data-image-height="296"
data-image-alt="FIG. 3"
data-pmc-id="PMC87107"
data-figure-id="F3">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-2"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/0b3afa336c48/mb1410490003.gif"
alt="FIG. 3" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="552">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="296">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 3 </p>
</strong>
<div class="figure-caption-contents"><p> PLIP interacts with cPLA <sub> 2… </sub> </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 3 </p>
</strong>
<div class="figure-caption-contents"><p> PLIP interacts with cPLA <sub> 2 </sub> . COS cells were transfected as follows: empty… </p></div>
</div>
<figcaption id="figure-caption-2" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 3
</strong>
<div class="figure-caption-contents">PLIP interacts with cPLA<sub>2</sub>. COS cells were transfected as follows: empty pMT<sub>3</sub> vector alone; pEGFP-cPLA<sub>2</sub> and pMT<sub>3</sub>; pEGFP-cPLA<sub>2</sub> and pMT<sub>3</sub>-PLIP; and pEGFP-cPLA<sub>2</sub> and pMT<sub>3</sub>-Tip60. Cell lysate proteins were separated by SDS-polyacrylamide gel electrophoresis (PAGE) and analyzed by Western blot analysis. Western blot with anti-cPLA<sub>2</sub> antibody demonstrates a band at approximately 112 kDa in lanes 6, 7, and 8, which contain lysates of pEGFP-cPLA<sub>2</sub>-transfected COS cells (arrow). Western blot with anti-HA antibody demonstrates a band at approximately 50 kDa in lane 3, which contains the lysate of pMT<sub>3</sub>-PLIP-transfected cells (arrow), and at 60 kDa in lane 4, which contains the lysate of pMT<sub>3</sub>-Tip60-transfected COS cells (arrow). After immunoprecipitation of lysates with anti-HA antibody and resolution of the precipitants by SDS-PAGE, Western blot analysis with the anti-cPLA<sub>2</sub> antibody revealed a band corresponding to GFP-cPLA<sub>2</sub> in lysates of cells in which GFP-cPLA<sub>2</sub> was coexpressed with HA-PLIP (lane 11) and HA-Tip60 (lane 12) but not in lysates of cells transfected with pEGFP-cPLA<sub>2</sub> and pMT<sub>3</sub> (lane 10).</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="3"
data-label-slug="fig-4">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/ed409e2a285e/mb1410490004.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-3"
role="button"
data-image-width="792"
data-image-height="558"
data-image-alt="FIG. 4"
data-pmc-id="PMC87107"
data-figure-id="F4">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-3"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/4fccf01abbb7/mb1410490004.gif"
alt="FIG. 4" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="792">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="558">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 4 </p>
</strong>
<div class="figure-caption-contents"><p> PLIP and Tip60 colocalize with… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 4 </p>
</strong>
<div class="figure-caption-contents"><p> PLIP and Tip60 colocalize with cPLA <sub> 2 </sub> . Panels a to h are… </p></div>
</div>
<figcaption id="figure-caption-3" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 4
</strong>
<div class="figure-caption-contents">PLIP and Tip60 colocalize with cPLA<sub>2</sub>. Panels a to h are representative photographs which demonstrate colocalization of HA-tagged PLIP and cPLA<sub>2</sub> by immunofluorescence in transfected COS cells. Expressed proteins were identified with anti-cPLA<sub>2</sub> antibody followed by Cy3-conjugated anti-rabbit antibody. This was followed by anti-HA antibody followed by FITC-conjugated anti-mouse antibody. COS cells transfected with both pMT<sub>3</sub>-PLIP and pMT<sub>2</sub>-cPLA<sub>2</sub> are visualized with an FITC filter in panels e to h and a Cy3 filter in panels a to d. Panels i to l demonstrate cells expressing both Tip60 and cPLA<sub>2</sub>. cPLA<sub>2</sub> is visualized with a Cy3 filter in panels i and k, and Tip60 is visualized with an FITC filter in panels j and l.</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="4"
data-label-slug="fig-5">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/5b3b7f54995d/mb1410490005.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-4"
role="button"
data-image-width="568"
data-image-height="557"
data-image-alt="FIG. 5"
data-pmc-id="PMC87107"
data-figure-id="F5">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-4"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/f6d8e40cfca6/mb1410490005.gif"
alt="FIG. 5" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="568">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="557">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 5 </p>
</strong>
<div class="figure-caption-contents"><p> Detection and coimmunoprecipitation of Tip60-PLIP… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 5 </p>
</strong>
<div class="figure-caption-contents"><p> Detection and coimmunoprecipitation of Tip60-PLIP and cPLA <sub> 2 </sub> in cultured mesangial cells. (a)… </p></div>
</div>
<figcaption id="figure-caption-4" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 5
</strong>
<div class="figure-caption-contents">Detection and coimmunoprecipitation of Tip60-PLIP and cPLA<sub>2</sub> in cultured mesangial cells. (a) Western blot of lysates from COS, 293, ecv304, MDCK, and mesangial cells using a polyclonal antibody raised in rabbits against GST-PLIP. A band at approximately 50 kDa is present in the lane containing lysates of HA-PLIP-transfected COS cells. A band of approximately similar size is present in the lane corresponding to rat renal mesangial cells but not in those lanes containing protein from 293, ECV304, MDCK, or nontransfected COS cells. There is a light band in each lane at 60 kDa which likely reflects Tip60. (b) Gel electrophoresis of DNA obtained by RT-PCR of RNA harvested from BALB/c mouse tissue. Two bands of 456 and 300 bp, representing Tip60 and PLIP DNA, respectively, were obtained using primers which flanked exon 5. Molecular mass markers are indicated. (c) Western blot of rat neonatal myocytes infected with Ad-GFP or with increasing amounts of Ad-PLIP. Two bands are present in all lysates at approximately 60 and 50 kDa, representing Tip60 and PLIP, respectively. Signal corresponding to PLIP is enhanced in lysates of Ad-PLIP-infected myocytes at 350 PFU/cell. (d) Mesangial cell lysate was immunoprecipitated over 4 h with anti-PLIP antibody versus a control antibody which recognizes the nuclear protein, KRIP (43), or with anti-cPLA<sub>2</sub> antibody. Immunoprecipitants separated by SDS-polyacrylamide gel electrophoresis and transferred to an Immobilon membrane were blotted with cPLA<sub>2</sub> antibody. A band corresponding to cPLA<sub>2</sub> appears in lanes containing precipitants with either anti-PLIP or anti-cPLA<sub>2</sub> antibody but not with anti-KRIP antibody.</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="5"
data-label-slug="fig-6">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/07d2110b5862/mb141049006a.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-5"
role="button"
data-image-width="800"
data-image-height="644"
data-image-alt="FIG. 6"
data-pmc-id="PMC87107"
data-figure-id="F6">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-5"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/ac1123677ae5/mb141049006a.gif"
alt="FIG. 6" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="800">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="644">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 6 </p>
</strong>
<div class="figure-caption-contents"><p> Serum deprivation alters the nuclear… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 6 </p>
</strong>
<div class="figure-caption-contents"><p> Serum deprivation alters the nuclear localization of Tip60 or PLIP and cPLA <sub> 2… </sub> </p></div>
</div>
<figcaption id="figure-caption-5" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 6
</strong>
<div class="figure-caption-contents">Serum deprivation alters the nuclear localization of Tip60 or PLIP and cPLA<sub>2</sub>. (a) Rat renal mesangial cells grown on coverslips were fixed and exposed to anti-PLIP antibody followed by rhodamine-conjugated anti-rabbit antibody. Greater than 95% of mesangial cells grown in 0.25% FCS (fcs) (panels 5 and 6) demonstrate nuclear localization of Tip60 or PLIP by immunofluorescence microscopy as compared to 2 to 4% of cells grown in 10% FCS (+fcs) for 48 h (panels 1 and 2). PLIP appears in the nuclei of approximately 12 to 24% of cells grown in 0.25% FCS for 24 h. (b) Mesangial cells were fixed and exposed to anti-cPLA<sub>2</sub> antibody followed by rhodamine-conjugated anti-rabbit antibody; cPLA<sub>2</sub>-associated signal is seen in the cytosol and nucleus in cells grown in 10% FCS (panels 1 and 2) but is lost from the cytosol and accentuated at the nucleus in cells grown in 0.25% FCS for 48 h (panels 3 and 4).</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="6"
data-label-slug="fig-6">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/5e79f3bb8838/mb141049006b.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-6"
role="button"
data-image-width="567"
data-image-height="717"
data-image-alt="FIG. 6"
data-pmc-id="PMC87107"
data-figure-id="F6">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-6"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/ac1123677ae5/mb141049006a.gif"
alt="FIG. 6" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="567">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="717">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 6 </p>
</strong>
<div class="figure-caption-contents"><p> Serum deprivation alters the nuclear… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 6 </p>
</strong>
<div class="figure-caption-contents"><p> Serum deprivation alters the nuclear localization of Tip60 or PLIP and cPLA <sub> 2… </sub> </p></div>
</div>
<figcaption id="figure-caption-6" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 6
</strong>
<div class="figure-caption-contents">Serum deprivation alters the nuclear localization of Tip60 or PLIP and cPLA<sub>2</sub>. (a) Rat renal mesangial cells grown on coverslips were fixed and exposed to anti-PLIP antibody followed by rhodamine-conjugated anti-rabbit antibody. Greater than 95% of mesangial cells grown in 0.25% FCS (fcs) (panels 5 and 6) demonstrate nuclear localization of Tip60 or PLIP by immunofluorescence microscopy as compared to 2 to 4% of cells grown in 10% FCS (+fcs) for 48 h (panels 1 and 2). PLIP appears in the nuclei of approximately 12 to 24% of cells grown in 0.25% FCS for 24 h. (b) Mesangial cells were fixed and exposed to anti-cPLA<sub>2</sub> antibody followed by rhodamine-conjugated anti-rabbit antibody; cPLA<sub>2</sub>-associated signal is seen in the cytosol and nucleus in cells grown in 10% FCS (panels 1 and 2) but is lost from the cytosol and accentuated at the nucleus in cells grown in 0.25% FCS for 48 h (panels 3 and 4).</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="7"
data-label-slug="fig-7">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/f60da8198b66/mb1410490007.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-7"
role="button"
data-image-width="744"
data-image-height="613"
data-image-alt="FIG. 7"
data-pmc-id="PMC87107"
data-figure-id="F7">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-7"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/b8ecb000cdb9/mb1410490007.gif"
alt="FIG. 7" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="744">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="613">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIG. 7 </p>
</strong>
<div class="figure-caption-contents"><p> Serum deprivation causes apoptosis. (a… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIG. 7 </p>
</strong>
<div class="figure-caption-contents"><p> Serum deprivation causes apoptosis. (a and b) Mesangial cells grown in 0.25% FCS… </p></div>
</div>
<figcaption id="figure-caption-7" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIG. 7
</strong>
<div class="figure-caption-contents">Serum deprivation causes apoptosis. (a and b) Mesangial cells grown in 0.25% FCS die at approximately 50 h. These photographs demonstrate light microscopy of cells grown in 10% FCS (a) versus 0.25% FCS (b) for 50 h. (c to l) TUNEL stain of adherent cells grown in 10% FCS (c to g) or 0.25% FCS (h to l) at 36 h.</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="8"
data-label-slug="fig-8">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/50e4/87107/da15cb7c6c0f/mb1410490008.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-8"
role="button"
data-image-width="413"
data-image-height="460"
data-image-alt="FIG. 8"
data-pmc-id="PMC87107"
data-figure-id="F8">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-8"
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<p> FIG. 8 </p>
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<div class="figure-caption-contents"><p> PLIP expression potentiates cPLA <sub> 2… </sub> </p></div>
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<p> FIG. 8 </p>
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<div class="figure-caption-contents"><p> PLIP expression potentiates cPLA <sub> 2 </sub> -induced cell susceptibility to injury by serum withdrawal.… </p></div>
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FIG. 8
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<div class="figure-caption-contents">PLIP expression potentiates cPLA<sub>2</sub>-induced cell susceptibility to injury by serum withdrawal. (a) Wild-type (+/+) and cPLA<sub>2</sub> knockout (/) mouse mesangial cells were infected with Ad-PLIP or Ad-Lac. Forty-eight hours after infection, cells were incubated in serum-free medium for 48 h. Ad-PLIP-infected (+/+) cells showed a greater number of apoptotic cells than Ad-Lac-infected (+/+) cells or either Ad-PLIP- or Ad-Lac-infected (/) cells. Ad-PLIP resulted in no change in the number of apoptotic cells in the (/) cells compared to Ad-Lac-infected (/) cells. (b) PGE<sub>2</sub> levels were greater in the supernatants of Ad-PLIP-infected (+/+) cells than in those of Ad-PLIP-infected (/) cells and Ad-Lac-infected (+/+) or (/) cells. PGE<sub>2</sub> levels were greater in supernatants of Ad-Lac-infected (+/+) cells than in those of Ad-Lac-infected (/) cells. , <i>P</i> &lt; 0.05 versus (+/+) cells infected with Ad-Lac and (/) cells infected with either Ad-PLIP or Ad-Lac; , <i>P</i> &lt; 0.05 versus (/) cells infected with Ad-Lac.</div>
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