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<li><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/workshop-details.html"> Workshop Details </a></li><li><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/workshop-video.html"> Workshop Video </a></li><li class="has-children"><div class="acnav__label"> <i class="arrow down"></i><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/PCR.html"> The Polymerase Chain Reaction </a></div><ul class="acnav__list acnav__list--level2" style="list-style-type: none; display: none;"><li class="li nav-item"><a class="notactive nav-link link10314" href="/ncbi/workshops/2023-09_Primer-BLAST/PCR.html#PCR">Advent of PCR</a></li><li class="li nav-item"><a class="notactive nav-link link10325" href="/ncbi/workshops/2023-09_Primer-BLAST/PCR.html#Primers">Designing Primers</a></li></ul></li><li><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/PB_description.html"> What is Primer-BLAST? </a></li><li><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/PB_access.html"> Accessing Primer-BLAST </a></li><li class="has-children"><div class="acnav__label"> <i class="arrow down"></i><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/interface.html"> The Interface </a></div><ul class="acnav__list acnav__list--level2" style="list-style-type: none; display: none;"><li class="li nav-item"><a class="notactive nav-link link10639" href="/ncbi/workshops/2023-09_Primer-BLAST/interface.html#Template">Input Sequences</a></li><li class="li nav-item"><a class="notactive nav-link link106410" href="/ncbi/workshops/2023-09_Primer-BLAST/interface.html#Exon">Exon/Intron Selection</a></li><li class="li nav-item"><a class="notactive nav-link link106511" href="/ncbi/workshops/2023-09_Primer-BLAST/interface.html#Specificity">Specificity Check</a></li><li class="li nav-item"><a class="notactive nav-link link106612" href="/ncbi/workshops/2023-09_Primer-BLAST/interface.html#Advanced">Advanced Parameters</a></li></ul></li><li class="has-children is-open"><div class="acnav__label"> <i class="arrow up"></i><a class="active" href="/ncbi/workshops/2023-09_Primer-BLAST/results.html"> Results Page </a></div><ul class="acnav__list acnav__list--level2" style="list-style-type: none; display: block;"><li class="li nav-item"><a class="notactive nav-link link107714" href="/ncbi/workshops/2023-09_Primer-BLAST/results.html#Results">Results</a></li><li class="li nav-item"><a class="notactive nav-link link107815" href="/ncbi/workshops/2023-09_Primer-BLAST/results.html#Recent">Recent Results</a></li></ul></li><li class="has-children"><div class="acnav__label"> <i class="arrow down"></i><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/examples.html"> Practice Examples </a></div><ul class="acnav__list acnav__list--level2" style="list-style-type: none; display: none;"><li class="li nav-item"><a class="notactive nav-link link108917" href="/ncbi/workshops/2023-09_Primer-BLAST/examples.html#Ex1">Example 1: MPO mRNA</a></li><li class="li nav-item"><a class="notactive nav-link link1081018" href="/ncbi/workshops/2023-09_Primer-BLAST/examples.html#Ex2">Example 2: MPO Exon</a></li><li class="li nav-item"><a class="notactive nav-link link1081119" href="/ncbi/workshops/2023-09_Primer-BLAST/examples.html#Ex3">Example 3: Whale myoglobins</a></li></ul></li><li><a class="notactive" href="/ncbi/workshops/2023-09_Primer-BLAST/job_ids.html"> Today's Job IDs </a></li>
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<h1>Primer-BLAST Results</h1>
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</div>
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<h2><a id="Results"></a>Results</h2>
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<p>Primer-BLAST provides a graphical overview that shows where the primers bind on the template. If you use an NCBI Reference Sequence the graphic shows biological features on the template such as the exon structure shown here.</p>
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<p>The report itself shows a report for each of the primer pairs, 10 pairs by default, with the predicted binding location, product size, and selected thermodynamic and specifity statisics. It also shows any potentially unintended products in the chosen database.</p>
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<br /><br /><img src="/ncbi/workshops/2022-10_Primer-BLAST/Images/Slide25_PB_results.JPG" alt="Image of Primer-BLAST results" width="900" /><br /><br /><br />
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<h2><a id="Recent"></a>Retrieving Recent Results</h2>
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<p>You can retrieve recent Primer-BLAST results if you have the job ID. Just click the "<a href="https://www.ncbi.nlm.nih.gov/tools/primer-blast/retrieve.html" target="_blank" rel="noopener">Retrieve recent results" link</a> on the result or submission page. Enter the job ID into the form. Job IDs for Primer-BLAST expire in about 24 hours. We will provide you a list of today's job IDs for the examples.<br /><br /><img src="/ncbi/workshops/2022-10_Primer-BLAST/Images/Slide28_PB_results.JPG" alt="Image of Primer-BLAST recent results form" width="900" /><br /><br /></p>
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<p class=”margin-top-5”><small>Last Reviewed: September 22, 2023</small></p>
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