733 lines
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Text
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Flow Cytometry Core Facility
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<h1>
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<span>Flow Cytometry Core Facility</span>
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<h2>About our work</h2>
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<div><div class="c-text">
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<p>The Flow Cytometry Core Facility at the National Eye Institute provides flow cytometry analytical and sorting equipment and services to the NEI Intramural community. Flow cytometry is a technique for counting and examining microscopic particles, such as cells and beads, by suspending them in a stream of fluid and passing them by a detection apparatus. The Core utilizes state-of-the-art sample preparation, data acquisition and analysis, and sorting procedures in providing service and supporting collaborative research projects. The Core provides training to students, fellows, and principal investigators at NEI in the theory and practical use of the technology. The training includes sample preparation, staining, and handling post sort.</p>
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<h3 class="c-section__heading">
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Equipment
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<ul><li>2 Becton-Dickinson FACS Calibur analyzers</li><li>1 Becton-Dickinson FACS Aria II high-speed sorter</li><li>2 Miltenyi Biotec MACSQuant analyzers</li></ul>
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Services
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<p>The facility offers comprehensive analytical and sorting services. Techniques used in the core include:</p><ul><li>Apoptosis</li><li>Cell Proliferation and Tracking</li><li>High Parameter Immunophenotyping</li><li>Molecular analysis via Luminex</li><li>Sorting for sterile culturing or DNA, RNA and Protein Analysis</li><li>Retinal Cell Analysis and Sorting</li><li>Viability</li></ul><p>We also have FlowJo and GemStone Software available for analysis.</p><p>The facility also offers training in:</p><ul><li>Advanced Experiments in Flow Cytometry</li><li>Analysis of Flow Cytometry Data</li><li>Basic theory of Flow Cytometry</li></ul>
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Luminex Sample Submission
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</h3>
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<div class="c-text">
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<p>This service is for NEI intramural only. Before using the Luminex instrument, please read and complete the following documents.</p><ul><li><a href="/sites/default/files/2019-07/ChecklistForRunningLuminexinNHLBIFlowCore.pdf">Sample form (PDF 170 KB)</a></li><li><a href="/sites/default/files/2019-07/PlateLayoutForm.pdf">Plate layout form (PDF 351 KB)</a></li></ul>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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<div class="c-group__staff-list c-section" id="key-staff">
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<h2>Flow Cytometry Core Facility key staff</h2>
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<div class="views-element-container">
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<div class="js-view-dom-id-a3b4d02f952fb647dd1d9169da079bb433074e7645814329f8e0545654d21739 lab-staff">
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</div>
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</div>
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</div>
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<div class="c-group__staff-list c-section" id="alumni-staff">
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<div class="views-element-container">
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<div class="js-view-dom-id-8f5d8228090618e59fa1cd3ee571ee2f7228171291754352ad717e07d6c94549 lab-alumni">
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</div>
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</div>
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</div>
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</article>
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</div>
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<div class="views-element-container" id="block-views-block-news-page-block-5">
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<div>
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<div class="c-filter js-view-dom-id-8ebb0228367c60f5feec453f6232afc9f63808609ca6e02b66ed2811cebb55ba news-page news-page--block_5">
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</div>
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</div>
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</div>
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<div id="block-researchatnei" class="c-callout c-callout--wide c-callout--rounded">
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<figure class="c-callout__image">
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<img src="/sites/default/files/styles/400px_square_crop/public/2019-04/shutterstock_605501567_333.jpg?itok=yofmpBZG" alt="">
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</figure>
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<div class="c-callout__content">
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<h2 class="c-callout__heading">Research at NEI</h2>
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<ul><li><a href="/research/research-labs-and-branches">Research Labs and Branches</a></li><li><a href="/research/research-labs-and-branches/we-are-nei-intramural">Principal Investigators</a></li><li><a href="/grants-and-training/training-nei">Training at NEI</a></li><li><a href="/about/news-and-events/news">Research News</a></li></ul>
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</div>
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</div>
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<p class="views-element-container c-last-updated" id="block-views-block-nei-last-updated-block-1"><strong>Actualización más reciente:</strong> 27 de diciembre de 2024</p>
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</div>
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<aside class="r-sidebar" role="complementary">
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<div class="c-sidebar-widget">
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<div class="c-chief">
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<div class="c-chief__upper">
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<div class="views-element-container">
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<div class="o-table--sortable js-view-dom-id-475d27f38e196cfb05008a8d2a2cbfffc9207ae39ef598383505c55a3b1f27ee lab-staff">
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</div>
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</div>
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</div>
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</div>
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<div class="c-sidebar-widget__contact-information">
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<div class="c-sidebar-widget__header">
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<h2 class="c-sidebar-widget__title">Contact <span>Information</span></h2>
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</div>
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<div class="views-element-container">
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<div class="js-view-dom-id-b57af55e85452f45bd64b5276d864fdc6d55fd9f24504308598b32c7dc30da2e sidebar-address-labs">
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<div>
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<div class="c-sidebar-widget__contact-name">
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<span>Flow Cytometry Core Facility</span>
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</div>
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<div class="c-sidebar-widget__contact-address">
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10 Center Dr<br>
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<br>
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Bethesda,
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Maryland
|
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20892-1857
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</div>
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<div class="c-sidebar-widget__contact-phone-fax">
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<div>
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<div><span>Phone:</span> 301-435-4555</div>
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</div>
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</div>
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</div>
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</div>
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</div>
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<div class="c-sidebar-widget__contact-additional-info">
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<a href="#key-staff" class="c-link__link">See additional lab contact information</a>
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</div>
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</div>
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</div>
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</aside>
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</div>
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</main>
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<div class="r-footer">
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<footer class="o-footer" role="contentinfo">
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<div class="o-footer__row">
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<div class="o-footer__group o-footer__group--logo"><a class="o-logo__link" href="/" rel="home" title="Home"><span class="o-logo__text">National Eye Institute</span></a>
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<ul class="o-menu-social">
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<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--facebook outlink-ignore" href="https://www.facebook.com/NationalEyeInstitute">Facebook</a></li>
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<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--twitter outlink-ignore" href="https://twitter.com/NatEyeInstitute">Twitter</a></li>
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<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--linkedin outlink-ignore" href="https://www.linkedin.com/company/national-eye-institute-nei">LinkedIn</a></li>
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<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--youtube outlink-ignore" href="https://www.youtube.com/user/neinih">YouTube</a></li>
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</ul>
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<p> </p>
|
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</div>
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<div class="o-footer__group o-footer__group--contact">
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<h2 class="o-footer__title">Contactenos NEI</h2>
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<ul class="o-menu-footer">
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/contactenos">Contáctenos</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.nih.gov/about-nih/visitor-information">Visite a los NIH (en inglés)</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://list.nih.gov/cgi-bin/wa.exe?SUBED1=insidenei&A=1">Reciba actualizaciones del NEI a través del correo electrónico (en inglés)</a></li>
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</ul>
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</div>
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<div class="o-footer__group o-footer__group--policies">
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<h2 class="o-footer__title">Politicas</h2>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/informacion-sobre-accesibilidad">Accesibilidad</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.nih.gov/nondiscrimination-notice-language-assistance-services#spanish">Información en otros idiomas</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/ley-de-libertad-de-informacion-foia">Política de privacidad</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/ley-de-libertad-de-informacion-foia">Ley de Libertad de Información (FOIA)</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/descargo-de-responsabilidades">Descargo de responsabilidades</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.hhs.gov/vulnerability-disclosure-policy/index.html ">Declaración sobre vulnerabilidad (en inglés)</a></li>
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</div>
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<div class="o-footer__group o-footer__group--employees">
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<h2 class="o-footer__title">Para los empleados de NEI</h2>
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<ul class="o-menu-footer">
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://intranet.nei.nih.gov">Intranet</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://intranet.nei.nih.gov/hr/Pages/emergency.aspx">Información de emergencia</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://ned.nih.gov/search/search.aspx">Directorio de los funcionarios del NEI</a></li>
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<div class="o-footer__row">
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<div class="o-footer__full o-footer__full--parent">
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<ul class="o-menu-footer o-menu-footer--swing">
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.nih.gov/">NIH.gov</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://hhs.gov/">HHS.gov</a></li>
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<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://usa.gov/">USA.gov</a></li>
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