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<option value="abstract-text">Abstract (text)</option>
</select>
</div>
<div class="include-supplemental-container">
<input type="checkbox" aria-label="Include MeSH and other data" name="include-supplemental" id="email-include-supplemental" class="email-include-supplemental">
<label for="email-include-supplemental" class="email-include-supplemental-label">MeSH and other data</label>
</div>
<div class="form-field recaptcha ">
<div class="g-recaptcha" id="id-recaptcha" data-sitekey="6LfsWHMdAAAAAClKbtOpjQ2pMjgsGxvv7NdZW9uI"></div>
</div>
<div id="captcha-error-message" class="usa-input-error-message captcha-validation-message" role="alert"></div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Sending..."
data-ga-category="save_share"
data-ga-action="email"
data-ga-label="send">
Send email
</button>
<button class="action-panel-cancel"
aria-label="Close 'Email citations' panel"
ref="linksrc=close_email_panel"
aria-controls="email-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="email"
data-ga-label="cancel">
Cancel
</button>
</div>
<input type="hidden" name="email-search-details" value="" />
<input type="hidden" name="email-search-details-hash" value="0e42663a6c3bd85498fcb88798998fed7bfdc45d457db35281e41afe13cc0524" />
</form>
</div>
</div>
<div id="collections-action-panel"
class="collections-action-panel action-panel in-progress-dots-panel"
aria-hidden="true"
data-collections-open-panel-enabled="false"
data-collections-open-panel-url-hash="#open-collections-panel">
<div class="inner-wrap">
<h3 class="action-panel-heading">
Add to Collections
</h3>
<form id="collections-action-panel-form"
class="collections-action-panel-form action-panel-content action-form action-panel-smaller-selectors"
data-existing-collections-url="/list-existing-collections/"
data-add-to-existing-collection-url="/add-to-existing-collection/"
data-create-and-add-to-new-collection-url="/create-and-add-to-new-collection/"
data-get-article-ids-by-search-url="/get-article-ids-by-search/"
data-myncbi-max-collection-name-length="100"
data-add-to-collection-max-amount="1000"
data-collections-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/">
<input type="hidden" name="csrfmiddlewaretoken" value="unUhQpLXKlwBtbzDePMGovh30JWZCut8LaEmtfejkWj5nCFtR8pISDNHyNEbv51s">
<div class="choice-group" role="radiogroup">
<ul class="radio-group-items">
<li>
<input type="radio"
id="collections-action-panel-new"
class="collections-new"
name="collections"
value="new"
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="collections_radio_new">
<label for="collections-action-panel-new">Create a new collection</label>
</li>
<li>
<input type="radio"
id="collections-action-panel-existing"
class="collections-existing"
name="collections"
value="existing"
checked="true"
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="collections_radio_existing">
<label for="collections-action-panel-existing">Add to an existing collection</label>
</li>
</ul>
</div>
<div class="controls-wrapper">
<div class="action-panel-control-wrap new-collections-controls">
<label for="collections-action-panel-add-to-new" class="action-panel-label required-field-asterisk">
Name your collection:
</label>
<input
type="text"
name="add-to-new-collection"
id="collections-action-panel-add-to-new"
class="collections-action-add-to-new"
pattern="[^&quot;&amp;=&lt;&gt;\/]*" title="The following characters are not allowed in the Name field: &quot;&amp;=&lt;&gt;/"
maxlength="100"
data-ga-category="save_share"
data-ga-action="create_collection"
data-ga-label="non_favorties_collection">
<div class="collections-new-name-too-long usa-input-error-message selection-validation-message">
Name must be less than 100 characters
</div>
</div>
<div class="action-panel-control-wrap existing-collections-controls">
<label for="collections-action-panel-add-to-existing" class="action-panel-label">
Choose a collection:
</label>
<select id="collections-action-panel-add-to-existing"
class="action-panel-selector collections-action-add-to-existing"
data-ga-category="save_share"
data-ga-action="select_collection"
data-ga-label="($('#collections-action-add-to-existing').val() === 'Favorites') ? 'Favorites' : 'non_favorites_collection'">
</select>
<div class="collections-retry-load-on-error usa-input-error-message selection-validation-message">
Unable to load your collection due to an error<br>
<a href="#">Please try again</a>
</div>
</div>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Adding..."
data-pinger-ignore
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="add">
Add
</button>
<button class="action-panel-cancel"
aria-label="Close 'Add to Collections' panel"
ref="linksrc=close_collections_panel"
aria-controls="collections-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="collections"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="bibliography-action-panel"
class="bibliography-action-panel action-panel in-progress-dots-panel"
aria-hidden="true"
data-bibliography-open-panel-enabled="false"
data-bibliography-open-panel-url-hash="#open-bibliography-panel">
<div class="inner-wrap">
<h3 class="action-panel-heading">
Add to My Bibliography
</h3>
<form id="bibliography-action-panel-form"
class="bibliography-action-panel-form action-panel-content action-form action-panel-smaller-selectors"
data-add-to-bibliography-max-amount="100"
data-add-to-bibliography-batch-size="10"
data-bibliography-delegates-url="/list-bibliography-delegates/"
data-add-to-bibliography-url="/add-to-bibliography/"
data-get-article-ids-by-search-url="/get-article-ids-by-search/"
data-mybib-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/mybibliography/">
<input type="hidden" name="csrfmiddlewaretoken" value="unUhQpLXKlwBtbzDePMGovh30JWZCut8LaEmtfejkWj5nCFtR8pISDNHyNEbv51s">
<div class="action-panel-control-wrap bibliographies-controls">
<div class="choice-group">
<ul class="bibliographies-action-add radio-group-items">
<li>
<input name="bibliography" id="my-bibliography" class="my-bibliography" type="radio" checked/>
<label for="my-bibliography">My Bibliography</label>
</li>
</ul>
</div>
</div>
<div class="bibliographies-retry-load-on-error usa-input-error-message selection-validation-message">
Unable to load your delegates due to an error<br>
<a href="#">Please try again</a>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Adding..."
data-pinger-ignore>
Add
</button>
<button class="action-panel-cancel"
aria-label="Close 'Add to bibliography' panel"
ref="linksrc=close_bibliography_panel"
aria-controls="bibliography-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="mybib"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="saved-search-action-panel" class="saved-search-action-panel action-panel " aria-hidden="true"
data-saved-search-open-panel-enabled="false"
data-saved-search-open-panel-url-hash="#open-saved-search-panel">
<div class="inner-wrap">
<h2 class="action-panel-heading">
Your saved search
</h2>
<form id="saved-search-action-panel-form"
class="saved-search-action-panel-form action-panel-content action-form"
data-create-saved-search-url="/create-saved-search/"
data-try-search-terms-url="/try-search-term/"
data-saved-search-root-url="https://www.ncbi.nlm.nih.gov/myncbi/searches/">
<input type="hidden" name="csrfmiddlewaretoken" value="unUhQpLXKlwBtbzDePMGovh30JWZCut8LaEmtfejkWj5nCFtR8pISDNHyNEbv51s">
<div class="action-panel-control-wrap">
<label for="saved-search-name" class="action-panel-label saved-search-name-label required-field-asterisk">
Name of saved search:
</label>
<input maxlength="200"
type="text"
name="saved-search-name"
id="saved-search-name"
class="saved-search-name"
value=""
required
pattern="[^&quot;&amp;=&lt;&gt;\/]*" title="The following characters are not allowed in the Name field: &quot;&amp;=&lt;&gt;/">
</div>
<div class="action-panel-control-wrap">
<label for="saved-search-term" class="action-panel-label required-field-asterisk">
Search terms:
</label>
<textarea name="saved-search-term" id="saved-search-term" class="saved-search-term" required></textarea>
</div>
<div class="test-search-term-wrap">
<a href="#" class="try-search-term">Test search terms</a>
</div>
<div class="choice-group action-panel-extra-margin-top">
<span class="action-panel-label" id="fieldset-label">
Would you like email updates of new search results?
</span>
<fieldset id="saved-search-alert" aria-describedby="fieldset-label">
<legend class="usa-sr-only">Saved Search Alert Radio Buttons</legend>
<ul class="radio-group-items">
<li>
<input type="radio" id="saved-search-alert-yes" class="saved-search-alert-yes" name="saved-search-alert" value="yes" checked>
<label for="saved-search-alert-yes" class="action-panel-label">Yes</label>
</li>
<li>
<input aria-label="No radio input" type="radio" id="saved-search-alert-no" class="saved-search-alert-no" name="saved-search-alert" value="no">
<label for="saved-search-alert-no" class="action-panel-label">No</label>
</li>
</ul>
</fieldset>
</div>
<div class="alert-schedule-wrap">
<div class="action-panel-control-wrap">
<label class="action-panel-label">
Email:
</label>
<span aria-label="Email address" id="saved-search-email" class="action-panel-label"><span class="action-panel-label-bold"></span> (<a class="myncbi-account-settings" href="https://www.ncbi.nlm.nih.gov/account/settings/">change</a>)</span>
</div>
<div class="action-panel-control-wrap action-panel-extra-margin-top">
<label for="saved-search-frequency" class="action-panel-label">
Frequency:
</label>
<select id="saved-search-frequency" class="no-border-panel-selector saved-search-frequency">
<option value="monthly">Monthly</option>
<option value="weekly">Weekly</option>
<option value="daily">Daily</option>
</select>
</div>
<div class="action-panel-control-wrap saved-search-monthly-additional">
<label for="saved-search-monthly-on-day" class="action-panel-label">
Which day?
</label>
<select id="saved-search-monthly-on-day" class="no-border-panel-selector">
<option value="Sunday">The first Sunday</option>
<option value="Monday">The first Monday</option>
<option value="Tuesday">The first Tuesday</option>
<option value="Wednesday">The first Wednesday</option>
<option value="Thursday">The first Thursday</option>
<option value="Friday">The first Friday</option>
<option value="Saturday">The first Saturday</option>
<option value="day">The first day</option>
<option value="weekday">The first weekday</option>
</select>
</div>
<div class="action-panel-control-wrap saved-search-weekly-additional">
<label for="saved-search-weekly-on-day" class="action-panel-label">
Which day?
</label>
<select id="saved-search-weekly-on-day" class="no-border-panel-selector saved-search-weekly-on-day">
<option value="Sunday">Sunday</option>
<option value="Monday">Monday</option>
<option value="Tuesday">Tuesday</option>
<option value="Wednesday">Wednesday</option>
<option value="Thursday">Thursday</option>
<option value="Friday">Friday</option>
<option value="Saturday">Saturday</option>
</select>
</div>
<div class="action-panel-control-wrap">
<label for="saved-search-report" class="action-panel-label">
Report format:
</label>
<select id="saved-search-report" class="no-border-panel-selector saved-search-report">
<option value="DocSum">Summary</option>
<option value="DocSumText">Summary (text)</option>
<option value="Abstract">Abstract</option>
<option value="AbstractText">Abstract (text)</option>
<option value="MEDLINE">PubMed</option>
</select>
</div>
<div class="action-panel-control-wrap">
<label for="saved-search-amount" class="action-panel-label">
Send at most:
</label>
<select id="saved-search-amount" class="no-border-panel-selector saved-search-amount">
<option value="1">1 item</option>
<option value="5" selected>5 items</option>
<option value="10">10 items</option>
<option value="20">20 items</option>
<option value="50">50 items</option>
<option value="100">100 items</option>
<option value="200">200 items</option>
</select>
</div>
<div>
<input type="checkbox" id="saved-search-send-if-no-result" class="saved-search-send-if-no-result" name="saved-search-send-if-no-result">
<label for="saved-search-send-if-no-result" class="action-panel-label smaller-checkbox">
Send even when there aren't any new results
</label>
</div>
<div class="action-panel-control-wrap option-text-in-email-wrap">
<label for="saved-search-email-text" class="action-panel-label">
Optional text in email:
</label>
<textarea name="saved-search-email-text"
id="saved-search-email-text"
class="saved-search-email-text"></textarea>
</div>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Saving..."
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="save">
Save
</button>
<button class="action-panel-cancel"
aria-label="Close 'Your saved search' panel"
ref="linksrc=close_saved_search_panel"
aria-controls="saved-search-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="citation-manager-action-panel" class="citation-manager-action-panel action-panel" aria-hidden="true">
<div class="inner-wrap">
<h2 class="action-panel-heading">
Create a file for external citation management software
</h2>
<form id="citation-manager-action-panel-form"
class="action-panel-content action-form"
action="/results-export-ids/"
data-by-search-action="/results-export-search-data/"
data-by-ids-action="/results-export-ids/"
method="post"
data-by-search-method="post"
data-by-ids-method="post">
<input type="hidden" name="csrfmiddlewaretoken" value="unUhQpLXKlwBtbzDePMGovh30JWZCut8LaEmtfejkWj5nCFtR8pISDNHyNEbv51s">
<input name="results-format" type="hidden" value="pubmed"/>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Sending..."
data-ga-category="save_share"
data-ga-action="citation_manager"
data-ga-label="save">
Create file
</button>
<button class="action-panel-cancel"
aria-label="Close 'Send citations to citation manager' panel"
ref="linksrc=close_citation_manager_panel"
aria-controls="citation-manager-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="citation_manager"
data-ga-label="cancel">
Cancel
</button>
</div>
</form>
</div>
</div>
<div id="rss-action-panel" class="rss-action-panel action-panel " aria-hidden="true">
<div class="inner-wrap">
<h2 class="action-panel-heading">
Your RSS Feed
</h2>
<form id="rss-action-panel-form"
class="rss-action-panel-form action-panel-content action-form"
data-create-rss-feed-url="/create-rss-feed-url/"
data-search-form-term-value="">
<input type="hidden" name="csrfmiddlewaretoken" value="unUhQpLXKlwBtbzDePMGovh30JWZCut8LaEmtfejkWj5nCFtR8pISDNHyNEbv51s">
<div class="action-panel-control-wrap">
<label for="rss-name" class="action-panel-label required-field-asterisk">
Name of RSS Feed:
</label>
<input maxlength="200"
placeholder="Name"
type="text"
name="rss-name"
id="rss-name"
class="rss-name"
value=''
required
pattern="[^&quot;&amp;=&lt;&gt;\/]*" title="The following characters are not allowed in the Name field: &quot;&amp;=&lt;&gt;/">
</div>
<div class="rss-limit-wrap">
<div class="action-panel-control-wrap action-panel-extra-margin-top">
<label for="rss-limit" class="action-panel-label">
Number of items displayed:
</label>
<select id="rss-limit" class="no-border-panel-selector rss-limit">
<option value="5">5</option>
<option value="10">10</option>
<option value="15" selected="selected">15</option>
<option value="20">20</option>
<option value="50">50</option>
<option value="100">100</option>
</select>
</div>
</div>
<div class="action-panel-actions">
<button class="action-panel-submit"
type="submit"
data-loading-label="Creating..."
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="save">
Create RSS
</button>
<button class="action-panel-cancel"
aria-label="Close 'Your RSS' panel"
ref="linksrc=close_rss_panel"
aria-controls="rss-action-panel"
aria-expanded="false"
data-ga-category="save_share"
data-ga-action="alert"
data-ga-label="cancel">
Cancel
</button>
</div>
<div class="action-panel-control-wrap rss-link-copy-wrap">
<label for="rss-link" class="usa-sr-only">RSS Link</label>
<input placeholder="Your RSS Feed Link" type="text" name="rss-link" id="rss-link" class="rss-link" title="RSS Link">
<button
type="button"
disabled
class="rss-link-copy-button disabled"
data-ga-category="save_share"
data-ga-action="rss"
data-ga-label="copy">
Copy
</button>
</div>
</form>
</div>
</div>
</div>
</div>
<div class="article-page" id="article-page" data-article-pmid="33013788">
<aside class="page-sidebar">
<div class="inner-wrap">
<div class="full-text-links">
<div class="full-view">
<h3 class="title">
Full text links
</h3>
<div class="full-text-links-list">
<a class="link-item
dialog-focus"
href="https://doi.org/10.3389/fmicb.2020.557435"
target="_blank"
rel="noopener"
ref="linksrc=fulltextorjournal_fulltext&amp;is_pmc=False&amp;PrId=6956&amp;itool=Abstract-def&amp;log$=linkouticon&amp;uid=33013788&amp;db=pubmed&amp;nlmid=101548977"
title="See full text options at Frontiers Media SA"
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><img src="https://cdn.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--www.frontiersin.org-alerts-logo-PubMed.png" alt="Frontiers Media SA full text link"><span class="text">
Frontiers Media SA
</span></a><a class="link-item
pmc
"
href="https://pmc.ncbi.nlm.nih.gov/articles/pmid/33013788/"
target="_blank"
rel="noopener"
ref="linksrc=fulltextorjournal_fulltext&amp;is_pmc=True&amp;PrId=3494&amp;itool=Abstract-def&amp;log$=linkouticon&amp;uid=33013788&amp;db=pubmed&amp;nlmid=101548977"
title="Free full text at PubMed Central"
data-ga-category="full_text"
data-ga-action="PMC"
data-ga-label="33013788"
><span class="text">
Free PMC article
</span></a>
</div>
</div>
<div class="short-view">
<a href="#" class="full-text-links-button full-text-links-dialog-trigger">
Full text links
</a>
</div>
</div>
<div class="actions-buttons sidebar"><h3 class="title">Actions</h3><div class="inner-wrap"><button class="citation-button citation-dialog-trigger"
aria-label="Open dialog with citation text in different styles"
data-ga-category="save_share"
data-ga-action="cite"
data-ga-label="open"
data-all-citations-url="/33013788/citations/"
data-citation-style="nlm"
data-pubmed-format-link="/33013788/export/"><span class="button-label">Cite</span></button><link type="text/css" href="ncbi-overlay-block/src/overlay-block.css"><div class="collections-button-container" data-article-id="33013788" data-article-db="pubmed"><button class="collections-button collections-dialog-trigger"
aria-label="Save article in MyNCBI collections."
data-ga-category="collections_button"
data-ga-action="click"
data-ga-label="collections_button"
data-collections-open-dialog-enabled="false"
data-collections-open-dialog-url="https://account.ncbi.nlm.nih.gov/?back_url=https%3A%2F%2Fpubmed.ncbi.nlm.nih.gov%2F33013788%2F%23open-collections-dialog"
data-in-collections="false"><span class="button-label">Collections</span></button><div class="overlay" role="dialog"><div id="collections-action-dialog"
class="dialog collections-dialog"
aria-hidden="true"><div class="title">Add to Collections</div><div class="collections-action-panel action-panel"><form id="collections-action-dialog-form"
class="collections-action-panel-form action-panel-content action-form action-panel-smaller-selectors"
data-existing-collections-url="/list-existing-collections/"
data-add-to-existing-collection-url="/add-to-existing-collection/"
data-create-and-add-to-new-collection-url="/create-and-add-to-new-collection/"
data-myncbi-max-collection-name-length="100"
data-collections-root-url="https://www.ncbi.nlm.nih.gov/myncbi/collections/"><input type="hidden" name="csrfmiddlewaretoken" value="unUhQpLXKlwBtbzDePMGovh30JWZCut8LaEmtfejkWj5nCFtR8pISDNHyNEbv51s"><div class="choice-group" role="radiogroup"><ul class="radio-group-items"><li><input type="radio"
id="collections-action-dialog-new"
class="collections-new"
name="collections"
value="new"
data-ga-category="collections_button"
data-ga-action="click"
data-ga-label="collections_radio_new"><label for="collections-action-dialog-new">Create a new collection</label></li><li><input type="radio"
id="collections-action-dialog-existing"
class="collections-existing"
name="collections"
value="existing"
checked="true"
data-ga-category="collections_button"
data-ga-action="click"
data-ga-label="collections_radio_existing"><label for="collections-action-dialog-existing">Add to an existing collection</label></li></ul></div><div class="controls-wrapper"><div class="action-panel-control-wrap new-collections-controls"><label for="collections-action-dialog-add-to-new" class="action-panel-label required-field-asterisk">
Name your collection:
</label><input
type="text"
name="add-to-new-collection"
id="collections-action-dialog-add-to-new"
class="collections-action-add-to-new"
pattern="[^&quot;&amp;=&lt;&gt;\/]*" title="The following characters are not allowed in the Name field: &quot;&amp;=&lt;&gt;/"
maxlength="100"
data-ga-category="collections_button"
data-ga-action="create_collection"
data-ga-label="non_favorties_collection"><div class="collections-new-name-too-long usa-input-error-message selection-validation-message">
Name must be less than 100 characters
</div></div><div class="action-panel-control-wrap existing-collections-controls"><label for="collections-action-dialog-add-to-existing" class="action-panel-label">
Choose a collection:
</label><select id="collections-action-dialog-add-to-existing"
class="action-panel-selector collections-action-add-to-existing"
data-ga-category="collections_button"
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<h1 class="heading-title">
A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive <i>Vibrio harveyi</i> Biofilm
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A Mixture of D-Amino Acids Enhances the Biocidal Efficacy of CMIT/MIT Against Corrosive <i>Vibrio harveyi</i> Biofilm
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><sup class="key">1</sup> School of Life Sciences and Biopharmaceuticals, Shenyang Pharmaceutical University, Shenyang, China.</li>
<li data-affiliation-id="short-view-affiliation-2"
id="short-view-affiliation-2"
><sup class="key">2</sup> College of Life and Health Sciences, Northeastern University, Shenyang, China.</li>
<li data-affiliation-id="short-view-affiliation-3"
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><sup class="key">3</sup> Shenyang National Laboratory for Materials Science, Northeastern University, Shenyang, China.</li>
<li data-affiliation-id="short-view-affiliation-4"
id="short-view-affiliation-4"
><sup class="key">4</sup> Key Laboratory for Anisotropy and Texture of Materials (Ministry of Education), School of Materials Science and Engineering, Northeastern University, Shenyang, China.</li>
<li data-affiliation-id="short-view-affiliation-5"
id="short-view-affiliation-5"
><sup class="key">5</sup> Shenyang Aircraft Design and Research Institute, Shenyang, China.</li>
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Abstract
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<p>
Biocides are widely used for the mitigation of microbial contamination, especially in the field of the aviation fuel industry. However, the long-term use of biocide has raised the concerns regarding the environmental contamination and microbial drug resistance. In this study, the effect of a mixture of D-amino acids (D-tyrosine and D-methionine) on the enhancement of the bactericidal effect of 5-Chloro-2-Methyl-4-isothiazolin-3-one/2-Methyl-2H-isothiazole-3-one (CMIT/MIT) against corrosive <i>Vibrio harveyi</i> biofilm was evaluated. The results revealed that D-Tyr and D-Met alone can enhance the biocidal efficacy of CMIT/MIT, while the treatment of 5 ppm CMIT/MIT, 1 ppm D-Tyr and 100 ppm D-Met showed the best efficacy comparable to that of 25 ppm CMIT/MIT alone. The triple combination treatment successfully prevented the establishment of the corrosive <i>V. harveyi</i> biofilm and effectively removed the mature <i>V. harveyi</i> biofilm. These conclusions were confirmed by the results of sessile cell counts, images obtained by scanning electron microscope and confocal laser scanning microscope, and the ATP test kit.
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<p>
<strong class="sub-title">
Keywords:
</strong>
D-amino acid; D-methionine; D-tyrosine; Vibrio harveyi; biocide.
</p>
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<p class="copyright" id="copyright">
Copyright © 2020 Liu, Li, Fan, Lekbach, Song, Xu, Zhang, Ding and Wang.
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<figure class="figure-item "
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itemprop="associatedMedia"
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<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/a833cb5e57bf/fmicb-11-557435-g001.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-0"
role="button"
data-image-width="758"
data-image-height="295"
data-image-alt="FIGURE 1"
data-pmc-id="PMC7498826"
data-figure-id="F1">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-0"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/49196c21e472/fmicb-11-557435-g001.gif"
alt="FIGURE 1" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="758">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="295">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 1 </p>
</strong>
<div class="figure-caption-contents"><p> Electrochemical test: <b> (A) </b> Variation of… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 1 </p>
</strong>
<div class="figure-caption-contents"><p> Electrochemical test: <b> (A) </b> Variation of <i> R </i> <sub> <i> p </i> </sub> values during 7-day immersion, <b> (B) </b> Potentiodynamic… </p></div>
</div>
<figcaption id="figure-caption-0" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 1
</strong>
<div class="figure-caption-contents">Electrochemical test: <b>(A)</b> Variation of <i>R</i><sub><i>p</i></sub> values during 7-day immersion, <b>(B)</b> Potentiodynamic polarization curves of AA2024 coupons after 7-day immersion.</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="1"
data-label-slug="figure-2">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b5c0c7598527/fmicb-11-557435-g002.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-1"
role="button"
data-image-width="800"
data-image-height="1014"
data-image-alt="FIGURE 2"
data-pmc-id="PMC7498826"
data-figure-id="F2">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-1"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/2dba31fb5592/fmicb-11-557435-g002.gif"
alt="FIGURE 2" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="800">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1014">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 2 </p>
</strong>
<div class="figure-caption-contents"><p> SEM images of sessile cells… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 2 </p>
</strong>
<div class="figure-caption-contents"><p> SEM images of sessile cells after 3-day biofilm prevention tests: <b> (A) </b> no treatment… </p></div>
</div>
<figcaption id="figure-caption-1" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 2
</strong>
<div class="figure-caption-contents">SEM images of sessile cells after 3-day biofilm prevention tests: <b>(A)</b> no treatment (control), <b>(B)</b> 1 ppm <sc>D</sc>-Tyr, <b>(C)</b> 100 ppm <sc>D</sc>-Met, <b>(D)</b> 5 ppm CMIT/MIT, <b>(E)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr, <b>(F)</b> 5 ppm CMIT/MIT + 100 ppm <sc>D</sc>-Met, <b>(G)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr + 100 ppm <sc>D</sc>-Met, and <b>(H)</b> 25 ppm CMIT/MIT. Red arrows indicate the bacterial cells attached on AA2024 surfaces.</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="2"
data-label-slug="figure-3">
<a class="figure-link"
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itemprop="contentUrl"
aria-describedby="figure-caption-2"
role="button"
data-image-width="777"
data-image-height="287"
data-image-alt="FIGURE 3"
data-pmc-id="PMC7498826"
data-figure-id="F3">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-2"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/2f6fc0540760/fmicb-11-557435-g003.gif"
alt="FIGURE 3" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="777">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="287">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 3 </p>
</strong>
<div class="figure-caption-contents"><p> Sessile cell counts after 3-day… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 3 </p>
</strong>
<div class="figure-caption-contents"><p> Sessile cell counts after 3-day biofilm prevention test: <b> (A) </b> sessile cell number and… </p></div>
</div>
<figcaption id="figure-caption-2" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 3
</strong>
<div class="figure-caption-contents">Sessile cell counts after 3-day biofilm prevention test: <b>(A)</b> sessile cell number and <b>(B)</b> antibacterial rate. Standard deviations were from three independent experiments. <sup></sup><i>P</i> &lt; 0.001. There was no significant difference between group G and H (<i>P</i> &gt; 0.05).</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="3"
data-label-slug="figure-4">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/6db1d28872dd/fmicb-11-557435-g004.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-3"
role="button"
data-image-width="777"
data-image-height="289"
data-image-alt="FIGURE 4"
data-pmc-id="PMC7498826"
data-figure-id="F4">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-3"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/356828620cc4/fmicb-11-557435-g004.gif"
alt="FIGURE 4" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="777">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="289">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 4 </p>
</strong>
<div class="figure-caption-contents"><p> The amount of ATP on… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 4 </p>
</strong>
<div class="figure-caption-contents"><p> The amount of ATP on the AA2024 coupon surfaces after 3-day biofilm prevention… </p></div>
</div>
<figcaption id="figure-caption-3" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 4
</strong>
<div class="figure-caption-contents">The amount of ATP on the AA2024 coupon surfaces after 3-day biofilm prevention test: <b>(A)</b> ATP and <b>(B)</b> antibacterial rate. Standard deviations were from three independent experiments. ***<i>P</i> &lt; 0.001. There was no significant difference between group G and H (<i>P</i> &gt; 0.05).</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="4"
data-label-slug="figure-5">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/b4ec416ca149/fmicb-11-557435-g005.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-4"
role="button"
data-image-width="800"
data-image-height="1024"
data-image-alt="FIGURE 5"
data-pmc-id="PMC7498826"
data-figure-id="F5">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-4"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/51231cd76904/fmicb-11-557435-g005.gif"
alt="FIGURE 5" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="800">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1024">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 5 </p>
</strong>
<div class="figure-caption-contents"><p> CLSM images of sessile cells… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 5 </p>
</strong>
<div class="figure-caption-contents"><p> CLSM images of sessile cells after 3-day biofilm prevention test: <b> (A) </b> no treatment… </p></div>
</div>
<figcaption id="figure-caption-4" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 5
</strong>
<div class="figure-caption-contents">CLSM images of sessile cells after 3-day biofilm prevention test: <b>(A)</b> no treatment (control), <b>(B)</b> 1 ppm <sc>D</sc>-Tyr, <b>(C)</b> 100 ppm <sc>D</sc>-Met, <b>(D)</b> 5 ppm CMIT/MIT, <b>(E)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr, <b>(F)</b> 5 ppm CMIT/MIT + 100 ppm <sc>D</sc>-Met, <b>(G)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr + 100 ppm <sc>D</sc>-Met, and <b>(H)</b> 25 ppm CMIT/MIT.</div>
</figcaption>
</figure>
<figure class="figure-item "
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="5"
data-label-slug="figure-6">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/3386174c3529/fmicb-11-557435-g006.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-5"
role="button"
data-image-width="800"
data-image-height="1085"
data-image-alt="FIGURE 6"
data-pmc-id="PMC7498826"
data-figure-id="F6">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-5"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/87b3baf181dd/fmicb-11-557435-g006.gif"
alt="FIGURE 6" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="800">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1085">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 6 </p>
</strong>
<div class="figure-caption-contents"><p> EPS staining after biofilm prevention… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 6 </p>
</strong>
<div class="figure-caption-contents"><p> EPS staining after biofilm prevention test: <b> (A) </b> no treatment (control), <b> (B) </b> 1 ppm… </p></div>
</div>
<figcaption id="figure-caption-5" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 6
</strong>
<div class="figure-caption-contents">EPS staining after biofilm prevention test: <b>(A)</b> no treatment (control), <b>(B)</b> 1 ppm <sc>D</sc>-Tyr, <b>(C)</b> 100 ppm <sc>D</sc>-Met, <b>(D)</b> 5 ppm CMIT/MIT, <b>(E)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr, <b>(F)</b> 5 ppm CMIT + 100 ppm <sc>D</sc>-Met, <b>(G)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr + 100 ppm <sc>D</sc>-Met, and <b>(H)</b> 25 ppm CMIT/MIT. Blue, red and green dots represent extracellular DNAs, proteins, and polysaccharides, respectively.</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="6"
data-label-slug="figure-7">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/e57b4c205da7/fmicb-11-557435-g007.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-6"
role="button"
data-image-width="800"
data-image-height="1067"
data-image-alt="FIGURE 7"
data-pmc-id="PMC7498826"
data-figure-id="F7">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-6"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/643b2e667d77/fmicb-11-557435-g007.gif"
alt="FIGURE 7" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="800">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1067">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 7 </p>
</strong>
<div class="figure-caption-contents"><p> SEM images of sessile cells… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 7 </p>
</strong>
<div class="figure-caption-contents"><p> SEM images of sessile cells after the 1-day biofilm removal test: <b> (A) </b> no… </p></div>
</div>
<figcaption id="figure-caption-6" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 7
</strong>
<div class="figure-caption-contents">SEM images of sessile cells after the 1-day biofilm removal test: <b>(A)</b> no treatment (control), <b>(B)</b> 1 ppm <sc>D</sc>-Tyr, <b>(C)</b> 100 ppm <sc>D</sc>-Met, <b>(D)</b> 5 ppm CMIT/MIT, <b>(E)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr, <b>(F)</b> 5 ppm CMIT + 100 ppm <sc>D</sc>-Met, <b>(G)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr + 100 ppm <sc>D</sc>-Met, and <b>(H)</b> 25 ppm CMIT/MIT. Red arrows indicate the bacterial cells attached on AA2024 surfaces.</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="7"
data-label-slug="figure-8">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/2dc2f0c11f82/fmicb-11-557435-g008.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-7"
role="button"
data-image-width="777"
data-image-height="289"
data-image-alt="FIGURE 8"
data-pmc-id="PMC7498826"
data-figure-id="F8">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-7"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/50610b67f0a9/fmicb-11-557435-g008.gif"
alt="FIGURE 8" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="777">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="289">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 8 </p>
</strong>
<div class="figure-caption-contents"><p> Sessile cell counts on the… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 8 </p>
</strong>
<div class="figure-caption-contents"><p> Sessile cell counts on the AA2024 coupon surfaces after 1-day biofilm removal test:… </p></div>
</div>
<figcaption id="figure-caption-7" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 8
</strong>
<div class="figure-caption-contents">Sessile cell counts on the AA2024 coupon surfaces after 1-day biofilm removal test: <b>(A)</b> sessile cell number and <b>(B)</b> antibacterial rate. Standard deviations were from three independent experiments. ***<i>P</i> &lt; 0.001. There was no significant difference between group G and H (<i>P</i> &gt; 0.05).</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="8"
data-label-slug="figure-9">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/5382f6bb83d8/fmicb-11-557435-g009.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-8"
role="button"
data-image-width="772"
data-image-height="290"
data-image-alt="FIGURE 9"
data-pmc-id="PMC7498826"
data-figure-id="F9">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-8"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/34a83e5a85cd/fmicb-11-557435-g009.gif"
alt="FIGURE 9" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="772">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="290">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 9 </p>
</strong>
<div class="figure-caption-contents"><p> The amount of ATP on… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 9 </p>
</strong>
<div class="figure-caption-contents"><p> The amount of ATP on the AA2024 coupon surfaces after 1-day biofilm removal… </p></div>
</div>
<figcaption id="figure-caption-8" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 9
</strong>
<div class="figure-caption-contents">The amount of ATP on the AA2024 coupon surfaces after 1-day biofilm removal test: <b>(A)</b> ATP and <b>(B)</b> antibacterial rate. Standard deviations were from three independent experiments. ***<i>P</i> &lt; 0.001. There was no significant difference in group G and H (<i>P</i> &gt; 0.05).</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="9"
data-label-slug="figure-10">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/d39e2937b47b/fmicb-11-557435-g010.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-9"
role="button"
data-image-width="800"
data-image-height="1029"
data-image-alt="FIGURE 10"
data-pmc-id="PMC7498826"
data-figure-id="F10">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-9"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/aef020cf37d8/fmicb-11-557435-g010.gif"
alt="FIGURE 10" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="800">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1029">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 10 </p>
</strong>
<div class="figure-caption-contents"><p> CLSM images of sessile cells… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 10 </p>
</strong>
<div class="figure-caption-contents"><p> CLSM images of sessile cells after 1-day biofilm removal test: <b> (A) </b> no treatment… </p></div>
</div>
<figcaption id="figure-caption-9" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 10
</strong>
<div class="figure-caption-contents">CLSM images of sessile cells after 1-day biofilm removal test: <b>(A)</b> no treatment (control), <b>(B)</b> 1 ppm <sc>D</sc>-Tyr, <b>(C)</b> 100 ppm <sc>D</sc>-Met, <b>(D)</b> 5 ppm CMIT/MIT, <b>(E)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr, <b>(F)</b> 5 ppm CMIT/MIT + 100 ppm <sc>D</sc>-Met, <b>(G)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr + 100 ppm <sc>D</sc>-Met, and <b>(H)</b> 25 ppm CMIT/MIT.</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="10"
data-label-slug="figure-11">
<a class="figure-link"
href="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/258d0702f7f4/fmicb-11-557435-g011.jpg"
itemprop="contentUrl"
aria-describedby="figure-caption-10"
role="button"
data-image-width="800"
data-image-height="1201"
data-image-alt="FIGURE 11"
data-pmc-id="PMC7498826"
data-figure-id="F11">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-10"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/8650134125fa/fmicb-11-557435-g011.gif"
alt="FIGURE 11" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="800">
<meta itemprop="height" itemtype="http://schema.org/ImageObject" content="1201">
<div class="figure-caption figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="caption" aria-hidden="true">
<div class="caption-wrap">
<strong class="figure-label">
<p> FIGURE 11 </p>
</strong>
<div class="figure-caption-contents"><p> EPS staining after 1-day biofilm… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 11 </p>
</strong>
<div class="figure-caption-contents"><p> EPS staining after 1-day biofilm removal test: <b> (A) </b> no treatment (control), <b> (B) </b> 1… </p></div>
</div>
<figcaption id="figure-caption-10" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 11
</strong>
<div class="figure-caption-contents">EPS staining after 1-day biofilm removal test: <b>(A)</b> no treatment (control), <b>(B)</b> 1 ppm <sc>D</sc>-Tyr, <b>(C)</b> 100 ppm <sc>D</sc>-Met, <b>(D)</b> 5 ppm CMIT/MIT, <b>(E)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr, <b>(F)</b> 5 ppm CMIT/MIT + 100 ppm <sc>D</sc>-Met, <b>(G)</b> 5 ppm CMIT/MIT + 1 ppm <sc>D</sc>-Tyr + 100 ppm <sc>D</sc>-Met, and <b>(H)</b> 25 ppm CMIT/MIT. Blue, red, and green dots represent extracellular DNAs, proteins, and polysaccharides, respectively.</div>
</figcaption>
</figure>
<figure class="figure-item tail"
itemscope itemtype="http://schema.org/ImageObject"
itemprop="associatedMedia"
data-slide-index="11"
data-label-slug="figure-12">
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itemprop="contentUrl"
aria-describedby="figure-caption-11"
role="button"
data-image-width="770"
data-image-height="337"
data-image-alt="FIGURE 12"
data-pmc-id="PMC7498826"
data-figure-id="F12">
<img class="figure-thumb" itemprop="thumbnail"
id="article-image-11"
src="https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc3f/7498826/9d0e36ba24c4/fmicb-11-557435-g012.gif"
alt="FIGURE 12" />
</a>
<meta itemprop="width" itemtype="http://schema.org/ImageObject" content="770">
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<strong class="figure-label">
<p> FIGURE 12 </p>
</strong>
<div class="figure-caption-contents"><p> Schematic illustration of the synergy… </p></div>
</div>
</div>
<div class="figure-caption-medium figure-caption-text" aria-hidden="true">
<strong class="figure-label">
<p> FIGURE 12 </p>
</strong>
<div class="figure-caption-contents"><p> Schematic illustration of the synergy between <sc> D </sc> -amino acids and biocide. </p></div>
</div>
<figcaption id="figure-caption-11" class="figure-caption-full figure-caption-text" itemtype="http://schema.org/ImageObject" itemprop="description">
<strong class="figure-label">
FIGURE 12
</strong>
<div class="figure-caption-contents">Schematic illustration of the synergy between <sc>D</sc>-amino acids and biocide.</div>
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