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<article><div id="d-article"><div class="page-info"><div class="page-title"><a name="start" id="start">
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</a><h1 class="with-also" itemprop="name">Mononucleosis spot test</h1>
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</noscript></div><div class="main"><div id="ency_summary"><p>The mononucleosis spot test looks for 2 <a test="test" href="./002223.htm">antibodies</a> in the blood. These antibodies appear during or after an infection with the virus that causes mononucleosis, or mono.</p></div><section><div class="section"><div class="section-header"><div class="section-title"><h2>How the Test is Performed</h2></div><div class="section-button"><button type="submit" aria-controls="section-1" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-1"><p>A <a test="test" href="./003423.htm">blood sample</a> is needed. </p></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>How to Prepare for the Test</h2></div><div class="section-button"><button type="submit" aria-controls="section-2" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-2"><p>No special preparation is necessary.</p></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>How the Test will Feel</h2></div><div class="section-button"><button type="submit" aria-controls="section-3" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-3"><p>When the needle is inserted to draw blood, some people feel moderate pain. Others feel only a prick or stinging. Afterward, there may be some throbbing or a slight bruise. This soon goes away.</p></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>Why the Test is Performed</h2></div><div class="section-button"><button type="submit" aria-controls="section-4" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-4"><p>The mononucleosis spot test is done when symptoms of <a test="test" href="./000591.htm">mononucleosis</a> are present. Common symptoms include:</p><ul><li>Fatigue</li><li>Fever</li><li>Large spleen (possibly)</li><li>Sore throat</li><li>Tender lymph nodes along the back of the neck </li></ul><p>This test looks for antibodies called heterophile antibodies, which form in the body during the mononucleosis infection.</p></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>Normal Results</h2></div><div class="section-button"><button type="submit" aria-controls="section-5" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-5"><p>A negative test means there were no heterophile antibodies detected. Most of the time, this means you do not have infectious mononucleosis.</p><p>Sometimes, the test may be negative because it was done too soon (within 1 to 2 weeks) after the illness started. Your health care provider may repeat the test at a later date to make sure you do not have mono.</p></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>What Abnormal Results Mean</h2></div><div class="section-button"><button type="submit" aria-controls="section-6" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-6"><p>A positive test means heterophile antibodies are present. These are most often a sign of mononucleosis. Your provider will also consider other blood test results and your symptoms. A small number of people with mononucleosis may never have a positive test.</p><p>The highest number of antibodies occurs 2 to 5 weeks after mono begins. They may be present for up to 1 year.</p><p>In rare cases, the test is positive even though you do not have mono. This is called a false-positive result, and it may occur in people with:</p><ul><li>Hepatitis</li><li>Leukemia or lymphoma</li><li>Rubella</li><li>Systemic lupus erythematosus</li><li><a test="test" href="./000637.htm">Toxoplasmosis</a></li></ul></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>Risks</h2></div><div class="section-button"><button type="submit" aria-controls="section-7" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-7"><p>Veins and arteries vary in size from one person to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.</p><p>Other risks associated with having blood drawn are slight, but may include:</p><ul><li>Excessive bleeding</li><li>Fainting or feeling lightheaded</li><li>Hematoma (blood buildup under the skin)</li><li>Infection (a slight risk any time the skin is broken)</li></ul></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>Alternative Names</h2></div><div class="section-button"><button type="submit" aria-controls="section-Alt" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-Alt"><p>Monospot test; Heterophile antibody test; Heterophile agglutination test; Paul-Bunnell test; Forssman antibody test</p></div></div></section><section><div class="section sec-mb"><div class="section-header"><div class="section-title"><h2>Images</h2></div><div class="section-button"><button type="submit" aria-controls="section-tnails" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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<li><img src="//medlineplus.gov/ency/images/ency/tnails/1456t.jpg" alt="Mononucleosis - photomicrograph of cells" title="Mononucleosis - photomicrograph of cells" class="side-img"/><a href="../imagepages/1456.htm">Mononucleosis - photomicrograph of cells</a></li>
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<li><img src="//medlineplus.gov/ency/images/ency/tnails/2936t.jpg" alt="Mononucleosis - view of the throat" title="Mononucleosis - view of the throat" class="side-img"/><a href="../imagepages/2936.htm">Mononucleosis - view of the throat</a></li>
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<li><img src="//medlineplus.gov/ency/images/ency/tnails/9950t.jpg" alt="Throat swabs" title="Throat swabs" class="side-img"/><a href="../imagepages/9950.htm">Throat swabs</a></li>
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<li><img src="//medlineplus.gov/ency/images/ency/tnails/10026t.jpg" alt="Blood test" title="Blood test" class="side-img"/><a href="../imagepages/10026.htm">Blood test</a></li>
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<li><img src="//medlineplus.gov/ency/images/ency/tnails/9069t.jpg" alt="Antibodies" title="Antibodies" class="side-img"/><a href="../imagepages/9069.htm">Antibodies</a></li>
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</ul></div></div></section><section><div class="section"><div class="section-header"><div class="section-title"><h2>References</h2></div><div class="section-button"><button type="submit" aria-controls="section-Ref" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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</div></div><div class="section-body" id="section-Ref"><p>Johannsen EC, Kaye KM. Epstein-Barr virus (infectious mononucleosis, Epstein-Barr virus-associated malignant diseases, and other diseases). In: Bennett JE, Dolin R, Blaser MJ, eds. <em>Mandell, Douglas, and Bennett's Principles and Practice
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of Infectious Diseases.</em> 9th ed. Philadelphia, PA: Elsevier; 2020:chap 138.</p><p>Schooley RD, Allen UD. Epstein-Barr virus infection. In: Goldman L, Cooney KA, eds. <em>Goldman-Cecil Medicine</em>. 27th ed. Philadelphia, PA: Elsevier; 2024:chap 348.</p><p>Stillwell TL, Weinberg JB. Epstein-Barr virus. In: Kliegman RM, St. Geme JW, Blum NJ, et al, eds. <em>Nelson Textbook of Pediatrics</em>. 22nd ed. Philadelphia, PA: Elsevier; 2025:chap 301.</p></div></div></section>
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<section><div class="section"><div class="section-header"><div class="section-title"><h2>Review Date 3/16/2024</h2></div><div class="section-button"><button type="submit" aria-controls="section-version" role="button" title="Expand/Collapse section"><span class="icon icon-section-action">
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