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<h1 title="SAGE submissions">Recommendations for SAGE submissions</h1>
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Starting in January 2025, GEO will no longer accept SAGE submissions.
Please <a href="mailto:geo@ncbi.nlm.nih.gov">contact GEO</a> if you have any questions.
</p>
<p>
This page contains deposit recommendations, instructions and a template specific to
traditional (Sanger Sequencing) SAGE submission deposits. For instructions on submitting
data from next-generation sequencing instead, please refer to the
<a href="/geo/info/seq.html">Submitting high-throughput sequence data</a> page.
The following recommendations describe what we believe is the easiest batch
deposit method for the majority of researchers, but keep in mind that SAGE
data may be submitted to GEO using any of the deposit options described on the
<a href="/geo/info/submission.html">Submitting data</a> page.
If you need additional guidance for your submission, please <a href="mailto:geo@ncbi.nlm.nih.gov">email us</a>
with a brief description of the type of data you are trying to submit,
and one of our curators will quickly get back to you.
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The <a href="/geo/info/spreadsheet.html">GEOarchive</a> spreadsheet-based submission method is recommended for SAGE deposits.
With this submission option, you provide the following components:
</p>
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A Metadata worksheet containing descriptive information and protocols for the overall
experiment and individual libraries.
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<li>
One processed data file for each SAGE library included in your Metadata worksheet.
Files should be tab-delimited text files containing TAG (sequence) and COUNT (number
of times each tag is detected) columns only. Include all tags, including those with counts
of 1.
</li>
</ol>
<h2>SAGE GEOarchive templates and examples</h2>
<p>
The SAGE GEOarchive spreadsheet consists of 3 worksheets: a Metadata template, a Metadata example,
and an example of a formatted processed data file. Click the tabs at the bottom of the worksheet
window to switch between worksheets. Guidelines for the content of each field are provided on
the worksheets.
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<li><a href="/geo/info/examples/GA_sage.xls">Download SAGE GEOarchive spreadsheet</a></li>
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Bundle all components (Excel Metadata spreadsheet and processed data text files) together into a
.zip, .rar, or .tar archive using a program like WinZip, and transfer to GEO using the <a href="https://submit.ncbi.nlm.nih.gov/geo/submission/">Submit to GEO</a> page. Incomplete submissions will result in processing delays.
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Last modified: July 16, 2024</div>
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