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<script type="text/javascript" src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/jr.boots.min.js"> </script><title>ML322, A Small Molecule Inhibitor of Dimerization of the Core Protein of Hepatitis C Virus (HCV) - Probe Reports from the NIH Molecular Libraries Program - NCBI Bookshelf</title>
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<meta name="citation_inbook_title" content="Probe Reports from the NIH Molecular Libraries Program [Internet]">
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<meta name="citation_title" content="ML322, A Small Molecule Inhibitor of Dimerization of the Core Protein of Hepatitis C Virus (HCV)">
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<meta name="citation_publisher" content="National Center for Biotechnology Information (US)">
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<meta name="citation_date" content="2014/05/13">
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<meta name="citation_author" content="Smitha Kota">
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<meta name="citation_author" content="Franck Madoux">
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<meta name="citation_author" content="Peter Chase">
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<meta name="citation_author" content="Virginia Takahashi">
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<meta name="citation_author" content="Qin Liu">
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<meta name="citation_author" content="Becky A. Mercer">
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<meta name="citation_author" content="Michael Cameron">
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<meta name="citation_author" content="A. Donny Strosberg">
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<meta name="citation_author" content="William Roush">
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<meta name="citation_author" content="Peter Hodder">
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<meta name="citation_pmid" content="23833796">
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<meta name="citation_fulltext_html_url" content="https://www.ncbi.nlm.nih.gov/books/NBK148493/">
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<meta name="DC.Title" content="ML322, A Small Molecule Inhibitor of Dimerization of the Core Protein of Hepatitis C Virus (HCV)">
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<meta name="DC.Contributor" content="Smitha Kota">
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<meta name="DC.Contributor" content="Franck Madoux">
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<meta name="DC.Contributor" content="Peter Chase">
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<meta name="DC.Contributor" content="Virginia Takahashi">
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<meta name="DC.Contributor" content="Qin Liu">
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<meta name="DC.Contributor" content="Becky A. Mercer">
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<meta name="DC.Contributor" content="Michael Cameron">
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<meta name="DC.Contributor" content="A. Donny Strosberg">
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<meta name="DC.Contributor" content="William Roush">
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<meta name="DC.Contributor" content="Peter Hodder">
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<meta name="DC.Date" content="2014/05/13">
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<meta name="description" content="Over three million Americans are infected by the Hepatits C Virus (HCV) virus 2 which is particularly lethal for Acquired Immunodeficiency Syndrome (AIDS) patients, of whom increasing numbers are co-infected with Human Immunodeficiency Virus infection (HIV) and HCV. There is no vaccine, and the only available treatment, based on a combination of interferon and ribavirin, cures less than half of the patients. One of the important structural proteins of HCV is the core protein. HCV core has many reported functions in the host cells, but its main purpose for the virus is to enclose and thereby protect the HCV Ribonucleic acid (RNA) genome during disease transmission. The conserved nature of HCV and absence of a vaccine, along with studies demonstrating that core contributes to host cell oncogenesis, apoptosis inhibition, and suppression of host T cell responses, support a role for core as a major pathogenic component of HCV. As a result, the identification of specific inhibitors of HCV core dimerization may provide tools for inhibiting HCV assembly without host cell effects. Several studies identify HCV capsid assembly as an appropriate target for chemotherapeutic intervention of HCV infections. Thus, this High Throughput Screen (HTS) program was initiated to identify small molecules that inhibit HCV core protein dimerization. Following an HTS effort and several rounds of medicinal chemistry, we have identified compound CID 49800087 as inhibitor probe (ML322) belonging to the [1,2,4]triazolo[1,5-a]pyrimidin-2-yl scaffold. The probe inhibits dimerization of core proteins in biochemical Alphascreen assays (IC50 = 8.4 μM; 51% inhibition at 15 μM). Using quantitative polymerase chain reactions (QPCR) the probe was shown to reduce levels of total HCV RNA in infected cells (IC50 = 1.8 μM) and significantly reduces levels of infectious HCV RNA in cells treated with HCV-containing culture supernatants (IC50 = 2 nM). Profiling revealed that probe ML322 lacks activity against a panel of 63 receptors, transporters, and ion channels (Ricerca). Modest activity was detected at muscarinic M1-3, adrenergic alpha, and dopamine transporter (DAT). Critically, probe ML322 is not cytotoxic against the Huh7.5 liver cell line used for the infectivity assays (CC50 = 33 μM). This novel probe represents an exciting tool for use in basic HCV research to elucidate the mechanisms by which core-core dimerization contributes to HCV nucleocapsid formation, HCV infection, and liver cancers associated with HCV.">
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<meta name="og:description" content="Over three million Americans are infected by the Hepatits C Virus (HCV) virus 2 which is particularly lethal for Acquired Immunodeficiency Syndrome (AIDS) patients, of whom increasing numbers are co-infected with Human Immunodeficiency Virus infection (HIV) and HCV. There is no vaccine, and the only available treatment, based on a combination of interferon and ribavirin, cures less than half of the patients. One of the important structural proteins of HCV is the core protein. HCV core has many reported functions in the host cells, but its main purpose for the virus is to enclose and thereby protect the HCV Ribonucleic acid (RNA) genome during disease transmission. The conserved nature of HCV and absence of a vaccine, along with studies demonstrating that core contributes to host cell oncogenesis, apoptosis inhibition, and suppression of host T cell responses, support a role for core as a major pathogenic component of HCV. As a result, the identification of specific inhibitors of HCV core dimerization may provide tools for inhibiting HCV assembly without host cell effects. Several studies identify HCV capsid assembly as an appropriate target for chemotherapeutic intervention of HCV infections. Thus, this High Throughput Screen (HTS) program was initiated to identify small molecules that inhibit HCV core protein dimerization. Following an HTS effort and several rounds of medicinal chemistry, we have identified compound CID 49800087 as inhibitor probe (ML322) belonging to the [1,2,4]triazolo[1,5-a]pyrimidin-2-yl scaffold. The probe inhibits dimerization of core proteins in biochemical Alphascreen assays (IC50 = 8.4 μM; 51% inhibition at 15 μM). Using quantitative polymerase chain reactions (QPCR) the probe was shown to reduce levels of total HCV RNA in infected cells (IC50 = 1.8 μM) and significantly reduces levels of infectious HCV RNA in cells treated with HCV-containing culture supernatants (IC50 = 2 nM). Profiling revealed that probe ML322 lacks activity against a panel of 63 receptors, transporters, and ion channels (Ricerca). Modest activity was detected at muscarinic M1-3, adrenergic alpha, and dopamine transporter (DAT). Critically, probe ML322 is not cytotoxic against the Huh7.5 liver cell line used for the infectivity assays (CC50 = 33 μM). This novel probe represents an exciting tool for use in basic HCV research to elucidate the mechanisms by which core-core dimerization contributes to HCV nucleocapsid formation, HCV infection, and liver cancers associated with HCV.">
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id="jr-fip-info-p"><a id="jr-fip-prev" class="wsprkl btn" title="Jump to previuos match">◀</a><button id="jr-fip-matches">no matches yet</button><a id="jr-fip-next" class="wsprkl btn" title="Jump to next match">▶</a></nav></nav></div><div id="jr-epub-interstitial" class="hidden"></div><div id="jr-content"><article data-type="main"><div class="main-content lit-style" itemscope="itemscope" itemtype="http://schema.org/CreativeWork"><div class="meta-content fm-sec"><div class="fm-sec"><h1 id="_NBK148493_"><span class="title" itemprop="name">ML322, A Small Molecule Inhibitor of Dimerization of the Core Protein of Hepatitis C Virus (HCV)</span></h1><p class="contribs">Kota S, Madoux F, Chase P, et al.</p><p class="fm-aai"><a href="#_NBK148493_pubdet_">Publication Details</a></p></div></div><div class="jig-ncbiinpagenav body-content whole_rhythm" data-jigconfig="allHeadingLevels: ['h2'],smoothScroll: false" itemprop="text"><div id="_abs_rndgid_" itemprop="description"><p>Over three million Americans are infected by the Hepatits C Virus (HCV) virus 2 which is particularly lethal for Acquired Immunodeficiency Syndrome (AIDS) patients, of whom increasing numbers are co-infected with Human Immunodeficiency Virus infection (HIV) and HCV. There is no vaccine, and the only available treatment, based on a combination of interferon and ribavirin, cures less than half of the patients. One of the important structural proteins of HCV is the core protein. HCV core has many reported functions in the host cells, but its main purpose for the virus is to enclose and thereby protect the HCV Ribonucleic acid (RNA) genome during disease transmission. The conserved nature of HCV and absence of a vaccine, along with studies demonstrating that core contributes to host cell oncogenesis, apoptosis inhibition, and suppression of host T cell responses, support a role for core as a major pathogenic component of HCV. As a result, the identification of specific inhibitors of HCV core dimerization may provide tools for inhibiting HCV assembly without host cell effects. Several studies identify HCV capsid assembly as an appropriate target for chemotherapeutic intervention of HCV infections. Thus, this High Throughput Screen (HTS) program was initiated to identify small molecules that inhibit HCV core protein dimerization. Following an HTS effort and several rounds of medicinal chemistry, we have identified compound CID 49800087 as inhibitor probe (<a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=abstract&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>) belonging to the [<a class="bibr" href="#ml322.r1" rid="ml322.r1">1</a>,<a class="bibr" href="#ml322.r2" rid="ml322.r2">2</a>,<a class="bibr" href="#ml322.r4" rid="ml322.r4">4</a>]triazolo[1,5-<i>a</i>]pyrimidin-2-yl scaffold. The probe inhibits dimerization of core proteins in biochemical Alphascreen assays (IC50 = 8.4 μM; 51% inhibition at 15 μM). Using quantitative polymerase chain reactions (QPCR) the probe was shown to reduce levels of total HCV RNA in infected cells (IC50 = 1.8 μM) and significantly reduces levels of infectious HCV RNA in cells treated with HCV-containing culture supernatants (IC50 = 2 nM). Profiling revealed that probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=abstract&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> lacks activity against a panel of 63 receptors, transporters, and ion channels (Ricerca). Modest activity was detected at muscarinic M1-3, adrenergic alpha, and dopamine transporter (DAT). Critically, probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=abstract&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> is not cytotoxic against the Huh7.5 liver cell line used for the infectivity assays (CC50 = 33 μM). This novel probe represents an exciting tool for use in basic HCV research to elucidate the mechanisms by which core-core dimerization contributes to HCV nucleocapsid formation, HCV infection, and liver cancers associated with HCV.</p></div><div class="h2"></div><p><b>Assigned Assay Grant #:</b> 1-X01-MH085709-01</p><p><b>Screening Center Name & PI:</b> The Scripps Research Institute Molecular Screening Center (SRIMSC), Hugh Rosen</p><p><b>Chemistry Center Name & PI:</b> SRIMSC, Hugh Rosen</p><p><b>Assay Submitter & Institution:</b> A. Donny Strosberg, Scripps Florida</p><p><b>PubChem Summary Bioassay Identifier:</b>
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<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/1911" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 1911</a></p><div id="ml322.s1"><h2 id="_ml322_s1_">Probe Structure & Characteristics</h2><div id="ml322.fu1" class="figure bk_fig"><div class="graphic"><img src="/books/NBK148493/bin/ml322fu1.jpg" alt="Probe ML322." /></div><h3><span class="title">Probe ML322</span></h3></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322tu1"><a href="/books/NBK148493/table/ml322.tu1/?report=objectonly" target="object" title="Table" class="img_link icnblk_img figpopup" rid-figpopup="figml322tu1" rid-ob="figobml322tu1"><img class="small-thumb" src="/books/NBK148493/table/ml322.tu1/?report=thumb" src-large="/books/NBK148493/table/ml322.tu1/?report=previmg" alt="Image " /></a><div class="icnblk_cntnt"><h4 id="ml322.tu1"><a href="/books/NBK148493/table/ml322.tu1/?report=objectonly" target="object" rid-ob="figobml322tu1">Table</a></h4></div></div></div><div id="ml322.s2"><h2 id="_ml322_s2_">1. Introduction</h2><p>The Hepatitis C virus (HCV) is a major cause of liver failure and hepatocellular cancer, with about 170 million people infected worldwide [<a class="bibr" href="#ml322.r1" rid="ml322.r1">1</a>]. In the United States over three million individuals are infected with the HCV virus 2, which is particularly lethal for AIDS patients, of whom increasing numbers are co-infected with HIV and HCV3 [<a class="bibr" href="#ml322.r2" rid="ml322.r2">2</a>]. There is currently no vaccine available [<a class="bibr" href="#ml322.r3" rid="ml322.r3">3</a>, <a class="bibr" href="#ml322.r4" rid="ml322.r4">4</a>], and the only available treatment, based on a combination of pegylated interferon and ribavirin, cures less than half of the patients.</p><p>The HCV has a small RNA genome that is directly translated by the infected host cell into a single precursor polyprotein that is processed by enzymatic cleavage into 10 proteins of diverse function. The most N-terminal 21kDa protein of this HCV polyprotein is the HCV core (C) protein, which is a highly basic, RNA-binding structural protein essential for assembly and packaging of the viral genome [<a class="bibr" href="#ml322.r5" rid="ml322.r5">5</a>]. Core protein is cleaved by a host peptidase and anchored to the host cell endoplasmic reticulum, where it undergoes further processing into its mature form [<a class="bibr" href="#ml322.r6" rid="ml322.r6">6</a>]. The N terminal portion of this mature C protein mediates viral assembly through homodimerization and formation of higher order complexes with viral RNA to form the nucleocapsid, while the hydrophobic C terminal interacts with envelope glycoproteins to form the infectious particle [<a class="bibr" href="#ml322.r7" rid="ml322.r7">7</a>].</p><p>Several drugs are under clinical investigation (Phase 2 or 3) as anti-viral therapy for HCV. These include inhibitors of NS5B polymerase, NS3/NS4A protease, and NS5A [<a class="bibr" href="#ml322.r3" rid="ml322.r3">3</a>]. However, potent, low nanomolar inhibitors of HCV infection based on the inhibition of core protein dimerization are unavailable. Thus, any small molecule probe identified will serve as an important step in the development of lead compounds for novel HCV therapies. The novel probe reported here, <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>, exhibits an IC<sub>50</sub> of 1.8 μM (T2 QPCR) for blocking the levels of total HCV RNA in cells. In addition the probe potently reduces the levels of infections HCV RNA in cells (TCID<sub>50</sub> = 2 nM). All sets of biochemical and cellular assay data were highly reproducible between batches and runs. Importantly, <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> also consistently lacks activity in the cytotoxicity counterscreen (CC<sub>50</sub> >30 μM), demonstrating that it does not significantly affect liver cell viability. Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> lacks significant inhibitory activity effects against the majority of therapeutic targets tested (Ricerca screening). Together these results demonstrate that probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> far surpasses the probe criteria set forth in the chemical probe development plan.</p></div><div id="ml322.s3"><h2 id="_ml322_s3_">2. Materials and Methods</h2><p>All chemical reagents and solvents were acquired from commercial vendors. All assay protocols are reported in the relevant PubChem AIDs, provided in <a class="figpopup" href="/books/NBK148493/table/ml322.t1/?report=objectonly" target="object" rid-figpopup="figml322t1" rid-ob="figobml322t1">Table 1</a>. Solubility, stability, and glutathione reactivity analyses were conducted in accordance with NIH guidelines. CYP450 inhibition and microsome stability analyses were performed as previously described [<a class="bibr" href="#ml322.r8" rid="ml322.r8">8</a>].</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t1"><a href="/books/NBK148493/table/ml322.t1/?report=objectonly" target="object" title="Table 1" class="img_link icnblk_img figpopup" rid-figpopup="figml322t1" rid-ob="figobml322t1"><img class="small-thumb" src="/books/NBK148493/table/ml322.t1/?report=thumb" src-large="/books/NBK148493/table/ml322.t1/?report=previmg" alt="Table 1. HCV Core Inhibitors Project (see also Summary AID 1911)." /></a><div class="icnblk_cntnt"><h4 id="ml322.t1"><a href="/books/NBK148493/table/ml322.t1/?report=objectonly" target="object" rid-ob="figobml322t1">Table 1</a></h4><p class="float-caption no_bottom_margin">HCV Core Inhibitors Project (see also Summary AID 1911). </p></div></div><div id="ml322.s4"><h3>2.1. Assays</h3><p><a class="figpopup" href="/books/NBK148493/table/ml322.t1/?report=objectonly" target="object" rid-figpopup="figml322t1" rid-ob="figobml322t1">Table 1</a> lists all PubChem AIDs for the HCV Core Inhibitor project. Descriptions of the assays follow the table.</p><div id="ml322.s5"><h4>Primary Assay</h4><div id="ml322.s6"><h5>2.1a. HCV Core Primary Assays (see AID <u>1899</u> (1X%INH), AID <u>2152</u> (3X %INH), AID <u>2159</u>(IC50), and AID <u>2488</u> (powders, assay provider’s lab)</h5><p>The purpose of this biochemical assay is to determine the ability of test compounds to prevent dimerization of the hydrophilic N-terminal domain (residues 1–106; core106) of the HCV core protein (9). In this assay, test compounds are incubated with N-terminally tagged GST-core106 and Flag-core106 peptides, followed by addition of a Europium cryptate-tagged anti-GST antibody and a XL-665-tagged anti-Flag antibody. Dimerization of the core106 peptides and subsequent antibody binding brings the antibody tags into close proximity, allowing FRET from the Europium donor to the XL-665 acceptor, resulting in an increase in well FRET. As designed, compounds that inhibit core106 dimerization will prevent the interaction of the tagged antibodies, blocking the transfer of energy from Europium to XL-665, and thus inhibiting well FRET. Test compounds were assayed in singlicate (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/1899" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 1899</a>) and triplicate (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2152" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2152</a>) at a final nominal concentration of 5 micromolar, in quadruplicate in a 10-point 1:3 dilution series starting at a nominal test concentration of 50 micromolar (Round 0: <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2159" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2159</a>, Round 1: <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2488" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2488</a>).</p><p>Prior to the start of the assay, 5 microliters of Assay Buffer (100 mM HEPES, 0.5 mM EDTA, 2.5 mM DTT, 200 mM Potassium Fluoride, 0.05% CHAPS, 0.05% BSA, pH 7.45, filtered at 0.22 micrometer) were dispensed into columns 1 and 2 of 1536-well assay plates. The remaining 46 columns were filled with 2 microliters of Assay Buffer supplemented with 225 ng/mL of Eu(K)-anti GST antibody and 42.5 nM of Flag-Core106. Next, 1 microliter of inhibition controls were dispensed into column 3 (12.5 micromolar unlabeled core106 protein, 100% inhibition), column 46 (200 nM core106 protein, 50% inhibition), and columns 4 to 45, 47 and 48 (Assay Buffer alone). Twenty-five nL of 10-points serial dilutions of test compounds or DMSO alone (0.5% final concentration) were then added to the appropriate wells. Next, 2 microliters of Assay Buffer supplemented with 2.5 micrograms/mL XL665-anti FLAG antibody and 33.75 nM GST-Core106 were dispensed to columns 3 to 48. The assay plates were centrifuged for 30 seconds at 300g and incubated for 4 hours at 22.5 degrees Celsius. At the end of the incubation time, TR-FRET was measured by exciting the plates at 340nm, and monitoring well fluorescence at 617 nm (Eu) and 671 nm (XL665) with the ViewLux microplate reader (PerkinElmer). All wells had a final volume of 5 microliters. The final reagent concentrations were 90 ng/mL Eu(K)-anti GST antibody, 1 microgram/mL XL665-anti FLAG, 17 nM Flag-Core106 and 13.5 nM GST-Core106. Final concentrations of competing, untagged Core 106 proteins were 2.5 micromolar and 40 nM for the 100% and 50% inhibition controls, respectively.</p></div></div><div id="ml322.s7"><h4>Secondary Assays</h4><div id="ml322.s8"><h5>2.1b. Alphascreen Assays (see AID <u>463085</u>, AID <u>651576</u>, and AID <u>651577</u>)</h5><p>The purpose of this biochemical assay is to determine whether powder samples of compounds identified as possible HCV core probe candidates can disrupt the dimerization of HCV Core molecules (9). This assay employs the AlphaScreen technology, a secondary Amplified Luminescent Proximity Homogeneous Assay. This assay was run by the assay provider. Compounds were tested in duplicate at 15 micromolar (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651576" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651576</a>) and in triplicate in a 10-point series starting at a nominal test concentration of 50 micromolar (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/463085" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 463085</a> and <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651577" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651577</a>).</p><div id="ml322.f1" class="figure bk_fig"><div class="graphic"><img src="/books/NBK148493/bin/ml322f1.jpg" alt="Figure 1. Alphascreen Technology." /></div><h3><span class="label">Figure 1</span><span class="title">Alphascreen Technology</span></h3></div><p>AlphaScreen is based on the use of photoactive donor and acceptor beads that recognize specific tags on interacting proteins (10). Core106 dimerization was confirmed using AlphaScreen technology in which a core106 protein domain was tagged with either Glutathione-S-transferase (GST) tag or a Flag peptide tag. The untagged core106 protein domain was used as a model competitor in the assay. The proteins were diluted to working concentrations in ‘protein buffer’ (100 mM HEPES pH 7.5, 1 mM EDTA, 5 mM DTT, 0.1% CHAPS, 10% glycerol). The donor and acceptor beads were diluted to working concentrations in ‘bead buffer’ (20 mM HEPES pH7.5, 125 mM NaCl, 0.1% BSA, 0.1% CHAPS). GST-tagged core106 (150 nM) was incubated with 150 nM of Flag-tagged core106 for 1 hour at room temperature. Anti-Flag acceptor beads were added to the proteins at a final concentration of 20 μg/ml and incubated for 1 hour at room temperature. Then Glutathione donor beads were added to the proteins at a final concentration of 20 μg/ml and incubated for 1 hour. The assays were executed in a white 384 well Packard optiplate and were read on Perkin Elmer Envision.</p></div><div id="ml322.s9"><h5>2.1c. Cytotoxicity Assays (see AID <u>485280</u> and AID <u>651583</u>)</h5><p>The purpose of this assay is to determine whether powder samples of compounds identified as possible HCV core probe candidates are cytotoxic to Huh-7.5 cells. The assay utilizes compound XTT (2,3-bis[2-Methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt) with addition of an electron coupling agent phenazine methosulfate (PMS) to measure mitochondrial dehydrogenases activity in living cells. Dehydrogenases in mitochondria of living cells will cleave the tetrazolium ring of XTT changing the color of the solution from clear to orange. As designed, compounds that reduce cell viability will reduce activity of the mitochondrial dehydrogenases, thereby reducing the orange color in solution and well absorbance. Compounds were tested in triplicate in a 7-point 1:3 dilution series starting at a nominal test concentration of 320 μM (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485280" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485280</a> and <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651583" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651583</a>).</p><p>The Huh-7.5 cell line was routinely cultured in 15-cm dishes at 37 C and 95% relative humidity (RH). The growth media consisted of DMEM supplemented with 10% v/v certified fetal bovine serum, 4 mM L-glutamine, 1 mM sodium pyruvate, 1X antibiotic mix (penicillin, streptomycin, and glutamine), and 1X non-essential amino acids.</p><p>Prior to the start of the assay 8000 cells in 100 μL volume of colorless growth media were dispensed into each well of 96-well tissue culture-treated microtiter plates and incubated overnight at 37 C (5% CO2, 95% RH) to allow cells to adhere to the plate. The next day, 100 μL of test compound in DMSO (1.25 % final DMSO concentration) and DMSO only were added to wells. Wells with only test compound in media without cells were used to normalize the data (to account for the color a test compound may have). Next, the plates were incubated for 72 hours at 37 C (5% CO2, 95% RH). Solution of XTT-PMS was prepared in 1X PBS and added to the cells in 50 μL volume to each well and incubated for 4 hours at 37 C (5% CO2, 95% RH). After equilibrating the plates to room temperature for 10 minutes, optical density at 450 nm and 650 nm was measured on Biotek plate reader.</p></div><div id="ml322.s10"><h5>2.1d. Follow-up Assays to determine Selectivity and Cell-based Efficacy</h5><p>Additional assays were outsourced to Ricerca or were performed by the assay provider in order to elucidate the selectivity and cell-based efficacy of the probe candidates. These assays are explained in subsequent sections of this report. See <a href="#ml322.s25">Sections 3.5</a> & <a href="#ml322.s27">3.6</a> for details.</p></div></div></div><div id="ml322.s11"><h3>2.2. Probe Chemical Characterization</h3><p>The chemical structure of the probe was verified by analysis of its 400 MHz 1H NMR spectra obtained on a Brüker 400 MHz instrument; data are summarized in <a href="#ml322.s12">Section 2.3</a> (Probe Preparation). The chemical structure was also corroborated by its LC/MS molecular ion (calc form/z (M+1): 416.2454.4 and (m/z (M+Na)): 476.3, using an Agilent 1200/6140 multimode quadrupole rapid resolve system) as well as its expected fragmentation patterns. Purity was measured at >95% (LC/MS analysis, confirmed by analytical HLPC analysis). HPLC data was obtained using an Agilent 1200 analytical HPLC with an Agilent Eclipse XDB-C18 column, 2.1×50mm. The HPLC solvents used were acetonitrile and water (gradient 0–100% acetonitrile in 5 min) with 0.1% formic acid added to each mobile phase as the pH modifier. The retention time found was 1.23 min.</p><p>As shown in <a class="figpopup" href="/books/NBK148493/table/ml322.t2/?report=objectonly" target="object" rid-figpopup="figml322t2" rid-ob="figobml322t2">Table 2</a>, the solubility of probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> in PBS at pH 7.4 was determined to be ≥100 μM. Solubility was ≥100 μM in conditions reflective of a cell-based assay environment (DMEM, 10% FBS). Importantly, its solubility is fully adequate to provide the high potency seen in cell-based infectivity assays (0.1–10 μM) and is also adequate for broad use as a biological probe to be used in a variety of aqueous-based media.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t2"><a href="/books/NBK148493/table/ml322.t2/?report=objectonly" target="object" title="Table 2" class="img_link icnblk_img figpopup" rid-figpopup="figml322t2" rid-ob="figobml322t2"><img class="small-thumb" src="/books/NBK148493/table/ml322.t2/?report=thumb" src-large="/books/NBK148493/table/ml322.t2/?report=previmg" alt="Table 2. Probe Properties." /></a><div class="icnblk_cntnt"><h4 id="ml322.t2"><a href="/books/NBK148493/table/ml322.t2/?report=objectonly" target="object" rid-ob="figobml322t2">Table 2</a></h4><p class="float-caption no_bottom_margin">Probe Properties. </p></div></div><p>The probe has a half-life of >48 hours in PBS at room temperature when tested at 10 μM. Disappearance of the LC peak for the <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> is not altered by addition of excess glutathione, indicating that <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> is not thiol-reactive under physiologically-relevant conditions.</p><p><a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> is stable in DMSO solution at room temperature (no erosion of peak intensity after 7 days) and is also stable as a free base dry powder.</p><p><a class="figpopup" href="/books/NBK148493/table/ml322.t3/?report=objectonly" target="object" rid-figpopup="figml322t3" rid-ob="figobml322t3">Table 3</a> lists the samples and quantities of probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> and analogs submitted to the MLSMR.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t3"><a href="/books/NBK148493/table/ml322.t3/?report=objectonly" target="object" title="Table 3" class="img_link icnblk_img figpopup" rid-figpopup="figml322t3" rid-ob="figobml322t3"><img class="small-thumb" src="/books/NBK148493/table/ml322.t3/?report=thumb" src-large="/books/NBK148493/table/ml322.t3/?report=previmg" alt="Table 3. Probe and Analogs submitted to the MLSMR." /></a><div class="icnblk_cntnt"><h4 id="ml322.t3"><a href="/books/NBK148493/table/ml322.t3/?report=objectonly" target="object" rid-ob="figobml322t3">Table 3</a></h4><p class="float-caption no_bottom_margin">Probe and Analogs submitted to the MLSMR. </p></div></div></div><div id="ml322.s12"><h3>2.3. Probe Preparation (SR-1966; ML322)</h3><div id="ml322.f2" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%202.%20Synthesis%20of%20probe%20ML322.&p=BOOKS&id=148493_ml322f2.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img src="/books/NBK148493/bin/ml322f2.jpg" alt="Figure 2. Synthesis of probe ML322." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 2</span><span class="title">Synthesis of probe ML322</span></h3></div><div id="ml322.s13"><h4>Synthesis of diketone 3[<a class="bibr" href="#ml322.r9" rid="ml322.r9">9</a>, <a class="bibr" href="#ml322.r10" rid="ml322.r10">10</a>]</h4><p>To a solution of 2,4-pentadione <b>1</b> (1.0 g, 10 mmol) in acetone (10 mL) were successively added potassium carbonate (1.38 g, 10 mmol) and 4-methylbenzyl bromide <b>2</b> (1.85 g, 10 mmol). The mixture was stirred overnight at reflux and was quenched with water (10 mL). The aqueous phase was extracted with AcOEt and the combined organic phases were washed with brine, dried over Na<sub>2</sub>SO<sub>4</sub>, filtered and concentrated under reduced pressure. Purification of the crude product by flash chromatography on silica gel (hexanes/AcOEt: 4/1) afforded the known<sup>1,2</sup> diketone <b>3</b> (1.82 g, 89%). <sup>1</sup>H NMR (300 MHz, CDCl<sub>3</sub>) δ 16.81–16.83 (br. s, 0.5H), 6.97–7.15 (m, 4H), 3.99 (t, 0.5H, <i>J</i>= 7.3 Hz), 3.61 (s, 1H), 3.10 (d, 1H, <i>J</i>= 7.3 Hz), 2.30–2.32 (s, 3H), 2.12 (s, 3H), 2.06 (s, 3H).</p></div><div id="ml322.s14"><h4>Synthesis of ester 6 [<a class="bibr" href="#ml322.r11" rid="ml322.r11">11</a>]</h4><p>To a solution of NaOH (880 mg, 22 mmol) in water (50 mL) was added the commercially available 5-amino-3-mercapto-1,2,4-triazole (<b>4</b>) (2.32 g, 20 mmol). The mixture was stirred for 15 min at room temperature, then a solution of ethyl bromoacetate (3.34g, 20 mmol) in MeOH (5 mL) was added. The mixture was stirred for 2 h at room temperature. The solid in suspension was filtered and recrystallized from EtOH providing the known<sup>3</sup> ester <b>6</b> (2.0 g, 50%). <sup>1</sup>H NMR (400 MHz, DMSO-<i>d</i><sub><i>6</i></sub>) δ 11.87 (s, 1H), 6.04 (s, 2H), 4.05 (q, <i>J</i> = 7.2 Hz, 2H), 3.82 (s, 2H), 1.15 (t, <i>J</i> = 7.2 Hz, 3H).</p></div><div id="ml322.s15"><h4>Synthesis of ester 7</h4><p>To a solution of ester <b>6</b> (202 mg, 1 mmol) in acetic acid (5 mL) was added diketone <b>3</b> (204 mg, 1 mmol). The reaction was heated at 100°C for 24 h, then was concentrated under reduced pressure. Purification of the crude product by flash chromatography on silica gel (hexanes/AcOEt : 1/1) afforded ester <b>7</b> (160 mg, 43%). <sup>1</sup>H (400 MHz, CDCl<sub>3</sub>) δ 7.10 (d, <i>J</i> = 8.0 Hz, 2H), 6.89 (d, <i>J</i> = 8.0 Hz, 2H), 4.23 (q, <i>J</i> = 7.2 Hz, 2H), 4.12 (s, 2H), 4.07 (s, 2H), 2.73 (s, 3H), 2.55 (s, 3H), 2.32 (s, 3H), 1.29 (t, <i>J</i> = 7.2 Hz, 3H).</p></div><div id="ml322.s16"><h4>Synthesis of carboxylic acid 8</h4><p>To a 3N solution of NaOH (2 mL, 6 mmol) in THF (2 mL) was added ester <b>7</b> (160 mg, 0.43 mmol). The reaction mixture was stirred 5 h at room temperature, then was diluted with water (5 mL). The aqueous phase was extracted with EtOAc, then was acidified until pH 5 and was extracted twice with EtOAc. The combined organic phases were washed with brine, dried over Na<sub>2</sub>SO<sub>4</sub>, filtered and concentrated under reduced pressure to give acid <b>8</b> (143 mg, 97%). <sup>1</sup>H (400 MHz, CDCl<sub>3</sub>) δ 7.11 (d, <i>J</i> = 8.0 Hz, 2H), 6.89 (d, <i>J</i> = 8.0 Hz, 2H), 4.11 (s, 2H), 3.89 (s, 2H), 2.78 (s, 3H), 2.60 (s, 3H), 2.32 (s, 3H).</p></div><div id="ml322.s17"><h4>Synthesis of amide 10</h4><p>To a solution of acid <b>8</b> (50 mg, 0.15 mmol) and triethylamine (0.1 mL, 0.75 mmol) in CH<sub>2</sub>Cl<sub>2</sub> (1 mL) were successively added the hydrochloride salt of EDC (43 mg, 0.22 mmol), HOBt (30 mg, 0.22 mmol), and commercially available amine <b>9</b> (103 mg, 0.45 mmol). The reaction mixture was stirred at room temperature for 24 h and was directly subjected to column chromatography on silica gel (CH<sub>2</sub>Cl<sub>2</sub>/MeOH: 9/1). This provided amide <b>10</b> (75 mg, 90%). <sup>1</sup>H (400 MHz, CDCl<sub>3</sub>) δ 7.69 (broad s, 1H), 7.11 (d, <i>J</i> = 8.0 Hz, 2H), 6.89 (d, <i>J</i> = 8.0 Hz, 2H ), 4.08 (s, 2H), 3.91 (s, 2H), 3.35 (q, <i>J</i> = 5.8 Hz, 2H), 3.27 (m, 4H), 2.75 (s, 3H), 2.56 (s, 3H), 2.44 (t, <i>J</i> = 6.3 Hz, 2H), 2.34-2.27 (m, 7H), 1.43 (s, 9H).</p></div><div id="ml322.s18"><h4>Synthesis of SR-1966 (Probe ML322)</h4><p>To a solution of amide <b>10</b> (75 mg, 0.13 mmol) in CH<sub>2</sub>Cl<sub>2</sub> (3 mL) was added trifluoroacetic acid (0.5 mL). The reaction mixture was stirred overnight at room temperature, then was concentrated under reduced pressure. Purification of the crude product by flash column chromatography using a Biotage SNAP cartridge KP-NH (CH<sub>2</sub>Cl<sub>2</sub>/MeOH : 9/1) afforded <b>SR-1966</b> (56 mg, 95%). <sup>1</sup>H (400 MHz, CDCl<sub>3</sub>) δ 7.59 (t, <i>J</i> = 5.1 Hz, 1H), 7.1 (d, <i>J</i> = 8.1 Hz, 2H), 6.9 (d, <i>J</i> = 8.1 Hz, 2H), 4.09 (broad s, 2H), 3.93 (broad s, 2H), 3.35 (q, <i>J</i> = 5.9 Hz, 2H), 2.76 (s, 3H), 2.69 (dd, <i>J</i> = 4.49, 5.25 Hz, 4H), 2.58 (s, 3H), 2.42 (t, <i>J</i> = 6.2 Hz, 2H), 2.34-2.28 (m, 7H); <sup>13</sup>C (100 MHz, CDCl<sub>3</sub>) δ 168.8, 166.0, 164.8, 153.7, 144.6, 136.6, 133.8, 129.5 (2C), 127.3 (2C), 119.6, 56.8, 52.9 (2C), 45.2 (2C), 36.3, 34.4, 32.9, 24.0, 20.9, 14.2; IR (neat) 3293, 2943, 2816, 1663, 1613, 1511, 1377, 1367, 1341, 1295, 1158 cm<sup>−1</sup>. MS (ES−) <i>m/z</i> = 452 (found for C<sub>23</sub>H<sub>31</sub>N<sub>7</sub>OS-H<sup>+</sup>).</p></div></div></div><div id="ml322.s19"><h2 id="_ml322_s19_">3. Results</h2><p>The flow chart (<a class="figpopup" href="/books/NBK148493/figure/ml322.f3/?report=objectonly" target="object" rid-figpopup="figml322f3" rid-ob="figobml322f3">Figure 3</a>) outlines the results of the uHTS screening campaign for HCV Core dimerization inhibitors at the SRIMSC. Following primary HTS in singlicate to identify HCV Core dimerization inhibitors using a TR-FRET-based assay (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/1899" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 1899</a>), available MLSMR compounds were selected for confirmation testing in triplicate (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2152" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2152</a>). Available compounds that confirmed activity and were deemed not to be fluorescent artifacts were tested in dose response assays in triplicate (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2159" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2159</a>). Of the 128 compounds ordered, 24 were removed as being artifact, and 12 were not available from the MLSMR. The dose response identified 92 compounds as possible quenchers or fluorescent artifiacts. As a result the top 9 available non-optically active compounds were ordered for testing as powders for confirmation of activity.</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml322f3" co-legend-rid="figlgndml322f3"><a href="/books/NBK148493/figure/ml322.f3/?report=objectonly" target="object" title="Figure 3" class="img_link icnblk_img figpopup" rid-figpopup="figml322f3" rid-ob="figobml322f3"><img class="small-thumb" src="/books/NBK148493/bin/ml322f3.gif" src-large="/books/NBK148493/bin/ml322f3.jpg" alt="Figure 3. HCV Core dimerization HTS campaign overview." /></a><div class="icnblk_cntnt" id="figlgndml322f3"><h4 id="ml322.f3"><a href="/books/NBK148493/figure/ml322.f3/?report=objectonly" target="object" rid-ob="figobml322f3">Figure 3</a></h4><p class="float-caption no_bottom_margin">HCV Core dimerization HTS campaign overview. </p></div></div><p>The compounds with the highest potency in the HTS HCV Core FRET-based dose response assay (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2159" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2159</a>) are shown in <a class="figpopup" href="/books/NBK148493/table/ml322.t4/?report=objectonly" target="object" rid-figpopup="figml322t4" rid-ob="figobml322t4">Table 4</a>.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t4"><a href="/books/NBK148493/table/ml322.t4/?report=objectonly" target="object" title="Table 4" class="img_link icnblk_img figpopup" rid-figpopup="figml322t4" rid-ob="figobml322t4"><img class="small-thumb" src="/books/NBK148493/table/ml322.t4/?report=thumb" src-large="/books/NBK148493/table/ml322.t4/?report=previmg" alt="Table 4. Most potent scaffolds from the HTS campaign." /></a><div class="icnblk_cntnt"><h4 id="ml322.t4"><a href="/books/NBK148493/table/ml322.t4/?report=objectonly" target="object" rid-ob="figobml322t4">Table 4</a></h4><p class="float-caption no_bottom_margin">Most potent scaffolds from the HTS campaign. </p></div></div><p>Following cheminformatic analyses of the 92 compounds tested in HCV core titration HTS assays, fluorescence quenchers and fluorescent artifacts (non-optically active) were removed, resulting in 9 compounds with promising activity. Two compounds did not recapitulate activity.</p><p>Compounds identified as clean hits included <a href="https://pubchem.ncbi.nlm.nih.gov/substance/49716510" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 49716510</a> (CID 22422906/ SR-01000819396), <a href="https://pubchem.ncbi.nlm.nih.gov/substance/56317368" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 56317368</a> (CID 24979772), <a href="https://pubchem.ncbi.nlm.nih.gov/substance/56323176" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 56323176</a> (CID 9426403), <a href="https://pubchem.ncbi.nlm.nih.gov/substance/47193664" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 47193664</a> (CID 5280489), <a href="https://pubchem.ncbi.nlm.nih.gov/substance/56316484" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 56316484</a> (CID 24979394) (see <a class="figpopup" href="/books/NBK148493/figure/ml322.f6/?report=objectonly" target="object" rid-figpopup="figml322f6" rid-ob="figobml322f6">Figure 6</a>). Powder samples of 9 promising compounds were ordered by the SRIMSC and tested in the same FRET-based biochemical HCV core dimerization assay to confirm activity (<a class="figpopup" href="/books/NBK148493/table/ml322.t5/?report=objectonly" target="object" rid-figpopup="figml322t5" rid-ob="figobml322t5">Table 5</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2488" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2488</a>). Only CID 22422906 was active (IC<sub>50</sub> = 3.66 μM), and its potency was similar to that of the earlier (liquid) batch provided by the MLSMR (IC<sub>50</sub> = 9.86 μM, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2159" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2159</a>).</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml322f6" co-legend-rid="figlgndml322f6"><a href="/books/NBK148493/figure/ml322.f6/?report=objectonly" target="object" title="Figure 6" class="img_link icnblk_img figpopup" rid-figpopup="figml322f6" rid-ob="figobml322f6"><img class="small-thumb" src="/books/NBK148493/bin/ml322f6.gif" src-large="/books/NBK148493/bin/ml322f6.jpg" alt="Figure 6. Structures of prior art compounds SL201 and SL209." /></a><div class="icnblk_cntnt" id="figlgndml322f6"><h4 id="ml322.f6"><a href="/books/NBK148493/figure/ml322.f6/?report=objectonly" target="object" rid-ob="figobml322f6">Figure 6</a></h4><p class="float-caption no_bottom_margin">Structures of prior art compounds SL201 and SL209. </p></div></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t5"><a href="/books/NBK148493/table/ml322.t5/?report=objectonly" target="object" title="Table 5" class="img_link icnblk_img figpopup" rid-figpopup="figml322t5" rid-ob="figobml322t5"><img class="small-thumb" src="/books/NBK148493/table/ml322.t5/?report=thumb" src-large="/books/NBK148493/table/ml322.t5/?report=previmg" alt="Table 5. The top Round 0 analog results." /></a><div class="icnblk_cntnt"><h4 id="ml322.t5"><a href="/books/NBK148493/table/ml322.t5/?report=objectonly" target="object" rid-ob="figobml322t5">Table 5</a></h4><p class="float-caption no_bottom_margin">The top Round 0 analog results. </p></div></div><p>Samples of <a href="https://pubchem.ncbi.nlm.nih.gov/substance/56317368" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 56317368</a> and <a href="https://pubchem.ncbi.nlm.nih.gov/substance/56323176" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 56323176</a> that were synthesized in our laboratory had activity far less than the HTS and were not pursued further. <a href="https://pubchem.ncbi.nlm.nih.gov/substance/56316484" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 56316484</a> (CID 24979394) was not pursued owing to the weak activity from HTS (IC<sub>50</sub> = 48 μM), plus the presence of a labile ester bond was a concern. <a href="https://pubchem.ncbi.nlm.nih.gov/substance/47193664" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 47193664</a> (CID 5280489) was not pursued owing to the lack of a clear path for SAR. Finally, <a href="https://pubchem.ncbi.nlm.nih.gov/substance/855627" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 855627</a> (CID 1780) was identified as a “frequent hitter” active in numerous PubChem assays and was removed from further consideration.</p><p>Based on recapitulation of CID 22422906, this compound and purchased analogs were submitted to the assay provider for testing in Alphascreen assays at 15 micromolar and liver cell cytotoxicity assays. As shown in the first row of <a class="figpopup" href="/books/NBK148493/table/ml322.t5/?report=objectonly" target="object" rid-figpopup="figml322t5" rid-ob="figobml322t5">Table 5</a>, only CID 22422906 met the criteria of a probe candidate: non-toxic to Huh 7.5 liver cells (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485280" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485280</a>, CC<sub>50</sub> = 123 μM), active in a secondary amplified luminescent proximity homogeneous assay (Alphascreen <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/463085" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 463085</a>, IC<sub>50</sub> = 5 μM), and able to inhibit the levels of HCV RNA in infected cells treated with viral culture supernatants (QPCR assay, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485271" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485271</a>). CID 22422906 is active in only 15 of 288 PubChem Bioassays tested (5.2%); however, several of the assays are fluorescence- or FRET-based. As a result, further SAR was pursued. This compound belongs to the 1,2,4]triazolo[1,5-a]pyrimidin-2-yl scaffold.</p><div id="ml322.s20"><h3>Medicinal Chemistry to explore SAR of CID 22422906</h3><p>Due to the promising activity and lack of liver cell cytotoxicity of CID 22422906, the SRISMC purchased and synthesized additional analogs to explore the SAR. These analogs were submitted to the Strosberg lab for testing using an orthogonal Alphascreen-based biochemical assay at 15 micromolar and to determine potency (<a class="figpopup" href="/books/NBK148493/table/ml322.t6/?report=objectonly" target="object" rid-figpopup="figml322t6" rid-ob="figobml322t6">Table 6</a>).</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t6"><a href="/books/NBK148493/table/ml322.t6/?report=objectonly" target="object" title="Table 6" class="img_link icnblk_img figpopup" rid-figpopup="figml322t6" rid-ob="figobml322t6"><img class="small-thumb" src="/books/NBK148493/table/ml322.t6/?report=thumb" src-large="/books/NBK148493/table/ml322.t6/?report=previmg" alt="Table 6. Round 1 Analogs: Alphascreen results." /></a><div class="icnblk_cntnt"><h4 id="ml322.t6"><a href="/books/NBK148493/table/ml322.t6/?report=objectonly" target="object" rid-ob="figobml322t6">Table 6</a></h4><p class="float-caption no_bottom_margin">Round 1 Analogs: Alphascreen results. SL274 was ultimately claimed as probe ML322. </p></div></div></div><div id="ml322.s21"><h3>3.1. Summary of Screening Results</h3><p>The HTS campaign identified a number of potential probe precursors (HTS <a class="figpopup" href="/books/NBK148493/figure/ml322.f3/?report=objectonly" target="object" rid-figpopup="figml322f3" rid-ob="figobml322f3">Figure 3</a>). The series of efforts used to determine probe candidates and ultimately the probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> is summarized in the hit to probe optimization flow chart (<a class="figpopup" href="/books/NBK148493/figure/ml322.f5/?report=objectonly" target="object" rid-figpopup="figml322f5" rid-ob="figobml322f5">Figure 5</a>). Data from primary, confirmatory and concentration response for the HCV Core screen were subjected to cheminformatics analysis to determine what scaffolds have near-neighbor active and inactive analogs, indicating the potential for SAR.</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml322f5" co-legend-rid="figlgndml322f5"><a href="/books/NBK148493/figure/ml322.f5/?report=objectonly" target="object" title="Figure 5" class="img_link icnblk_img figpopup" rid-figpopup="figml322f5" rid-ob="figobml322f5"><img class="small-thumb" src="/books/NBK148493/bin/ml322f5.gif" src-large="/books/NBK148493/bin/ml322f5.jpg" alt="Figure 5. Probe development overview (post-HTS)." /></a><div class="icnblk_cntnt" id="figlgndml322f5"><h4 id="ml322.f5"><a href="/books/NBK148493/figure/ml322.f5/?report=objectonly" target="object" rid-ob="figobml322f5">Figure 5</a></h4><p class="float-caption no_bottom_margin">Probe development overview (post-HTS). </p></div></div><p>The most promising hits in HCV Core active scaffolds were then validated by assay data obtained using independent batches of test samples, purchased or synthesized in-house. Certain hits gave variable assay results upon re-supply. Others validated upon re-supply, with HCV Core IC<sub>50</sub> values within 2-fold of the HTS value. These were the hits of highest interest that progressed to SAR studies. Compounds were further prioritized as candidates for probe development based upon the SAR of purchased or synthesized analogs, based also upon the finding that certain compounds gave only low levels of HCV Core dimerization inhibition relative to other compounds (<a class="figpopup" href="/books/NBK148493/figure/ml322.f5/?report=objectonly" target="object" rid-figpopup="figml322f5" rid-ob="figobml322f5">Figure 5</a>).</p><p>As discussed in <a href="#ml322.s19">Section 3</a>, only CID 22422906 survived as a viable starting point for probe development. To explore this series, the Strosberg lab pursued additional counterscreening and cell-based efficacy testing (<a class="figpopup" href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-figpopup="figml322t7" rid-ob="figobml322t7">Table 7</a>). Also included in <a class="figpopup" href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-figpopup="figml322t7" rid-ob="figobml322t7">Table 7</a> are comparative data for two compounds, SL201 and SL209 (<a class="figpopup" href="/books/NBK148493/figure/ml322.f6/?report=objectonly" target="object" rid-figpopup="figml322f6" rid-ob="figobml322f6">Figure 6</a>), derived from prior work by the assay provider on the development of small molecule inhibitors of the hepatitis C virus by inhibition of Core dimerization [<a class="bibr" href="#ml322.r12" rid="ml322.r12">12</a>]. As shown by these comparative data, SL201 and SL209 are far less effective at inhibiting production of infectious HCV particles (IC<sub>50</sub> = 0.286 μM for SL209) than the probe compound (<a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a>; SR-1966, SL274) that has IC<sub>50</sub> = 2 nM in this assay.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t7"><a href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" title="Table 7" class="img_link icnblk_img figpopup" rid-figpopup="figml322t7" rid-ob="figobml322t7"><img class="small-thumb" src="/books/NBK148493/table/ml322.t7/?report=thumb" src-large="/books/NBK148493/table/ml322.t7/?report=previmg" alt="Table 7. Secondary assay results (Strosberg lab)." /></a><div class="icnblk_cntnt"><h4 id="ml322.t7"><a href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-ob="figobml322t7">Table 7</a></h4><p class="float-caption no_bottom_margin">Secondary assay results (Strosberg lab). SL274 was the most potent in cell-based HCV infectivity assays and was selected to be probe ML322. </p></div></div></div><div id="ml322.s22"><h3>3.2. Dose Response Curves for Probe</h3><p>As shown in <a class="figpopup" href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-figpopup="figml322t7" rid-ob="figobml322t7">Table 7</a>, <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> has robust on-target activity in biochemical and cell-based target assays. The probe is not cytotoxic to Huh7.5 liver cells, and is able to block HCV production in these same cells. The dose response curves for the probe in the HCV biochemical and cell-based assays are shown (<a class="figpopup" href="/books/NBK148493/figure/ml322.f7/?report=objectonly" target="object" rid-figpopup="figml322f7" rid-ob="figobml322f7">Figure 7</a>).</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml322f7" co-legend-rid="figlgndml322f7"><a href="/books/NBK148493/figure/ml322.f7/?report=objectonly" target="object" title="Figure 7" class="img_link icnblk_img figpopup" rid-figpopup="figml322f7" rid-ob="figobml322f7"><img class="small-thumb" src="/books/NBK148493/bin/ml322f7.gif" src-large="/books/NBK148493/bin/ml322f7.jpg" alt="Figure 7. Dose response curves for Probe ML322 in the Alphascreen (A), QPCR T1/T2 (B), and TCID50 assays (C)." /></a><div class="icnblk_cntnt" id="figlgndml322f7"><h4 id="ml322.f7"><a href="/books/NBK148493/figure/ml322.f7/?report=objectonly" target="object" rid-ob="figobml322f7">Figure 7</a></h4><p class="float-caption no_bottom_margin">Dose response curves for Probe ML322 in the Alphascreen (A), QPCR T1/T2 (B), and TCID50 assays (C). </p></div></div></div><div id="ml322.s23"><h3>3.3. Scaffold/Moiety Chemical Liabilities</h3><p>As discussed in the original Chemical Probe Development Plan (CPDP) for this project, hits were prioritized based on their SAR profile determined from HTS, follow-up assays and results of SAR by purchase. The potency, selectivity and chemical tractability of the hit series summarized in Sections 3.0 and <a href="#ml322.s21">3.1</a> were considered. Hits or scaffold series that possess potential chemical instability or toxicological problems were eliminated (e.g., <a href="https://pubchem.ncbi.nlm.nih.gov/substance/47193664" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 47193664</a> and <a href="https://pubchem.ncbi.nlm.nih.gov/substance/855627" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 855627</a>), as clear strategies could not be devised to address these issues for these two hits. The chemical series selected for optimization (e.g., <a href="https://pubchem.ncbi.nlm.nih.gov/substance/49716510" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 49716510</a>) has the potential to address lesions associated with existing probes, to meet the criteria for a successful probe as laid out in the CPDP, and to improve the state of the art in the field.</p><p>Synthetic tractability, chemical stability, stability in biological systems, drug-likeness [<a class="bibr" href="#ml322.r13" rid="ml322.r13">13</a>, <a class="bibr" href="#ml322.r14" rid="ml322.r14">14</a>], lead likeness [<a class="bibr" href="#ml322.r15" rid="ml322.r15">15</a>], lack of structural alerts [<a class="bibr" href="#ml322.r16" rid="ml322.r16">16</a>] and anticipated physical properties such as solubility are considered in the selection of hits for probe development.</p><p>The post-HTS analysis led to the identification of six compounds as the top hits (<a class="figpopup" href="/books/NBK148493/figure/ml322.f4/?report=objectonly" target="object" rid-figpopup="figml322f4" rid-ob="figobml322f4">Figure 4</a>). Of these, all but <a href="https://pubchem.ncbi.nlm.nih.gov/substance/49716510" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 49716510</a> were eliminated from further consideration owing to the limited opportunities for SAR expansion or the weakness of the original hit (see reasons summarized in red for each structure in <a class="figpopup" href="/books/NBK148493/figure/ml322.f4/?report=objectonly" target="object" rid-figpopup="figml322f4" rid-ob="figobml322f4">Figure 4</a>). <a href="https://pubchem.ncbi.nlm.nih.gov/substance/855627" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID855627</a> has also been eliminated from further consideration owing to its promiscuous nature in PubChem assays, and concerns about the chemical/biochemical stability of the polyacetylene unit.</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml322f4" co-legend-rid="figlgndml322f4"><a href="/books/NBK148493/figure/ml322.f4/?report=objectonly" target="object" title="Figure 4" class="img_link icnblk_img figpopup" rid-figpopup="figml322f4" rid-ob="figobml322f4"><img class="small-thumb" src="/books/NBK148493/bin/ml322f4.gif" src-large="/books/NBK148493/bin/ml322f4.jpg" alt="Figure 4. Selection of SID 49716510 as the starting point for SAR hit optimization." /></a><div class="icnblk_cntnt" id="figlgndml322f4"><h4 id="ml322.f4"><a href="/books/NBK148493/figure/ml322.f4/?report=objectonly" target="object" rid-ob="figobml322f4">Figure 4</a></h4><p class="float-caption no_bottom_margin">Selection of SID 49716510 as the starting point for SAR hit optimization. </p></div></div><p><a href="https://pubchem.ncbi.nlm.nih.gov/substance/49716510" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 49716510</a> was confirmed using a powder sample purchased from a commercial source. One round of SAR by purchase was performed (9 analogs were available), and several of the compounds were as active as, if not more active as <a href="https://pubchem.ncbi.nlm.nih.gov/substance/49716510" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 49716510</a>. Based on these results, the first round of analog synthesis was completed, and an additional ca. 20 <a href="https://pubchem.ncbi.nlm.nih.gov/substance/49716510" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 49716510</a> analogs were provided to the Strosberg lab. After HCV core dimerization inhibition data with those new analogs, a decision was made to resource the project for additional analog synthesis. Subsequent testing of synthesized analogs by the Strosberg lab revealed that CID 49800087 was the most promising probe candidate (<a class="figpopup" href="/books/NBK148493/figure/ml322.f8/?report=objectonly" target="object" rid-figpopup="figml322f8" rid-ob="figobml322f8">Figure 8</a>).</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml322f8" co-legend-rid="figlgndml322f8"><a href="/books/NBK148493/figure/ml322.f8/?report=objectonly" target="object" title="Figure 8" class="img_link icnblk_img figpopup" rid-figpopup="figml322f8" rid-ob="figobml322f8"><img class="small-thumb" src="/books/NBK148493/bin/ml322f8.gif" src-large="/books/NBK148493/bin/ml322f8.jpg" alt="Figure 8. Comparison of the structures of the original HTS lead and final Probe ML322." /></a><div class="icnblk_cntnt" id="figlgndml322f8"><h4 id="ml322.f8"><a href="/books/NBK148493/figure/ml322.f8/?report=objectonly" target="object" rid-ob="figobml322f8">Figure 8</a></h4><p class="float-caption no_bottom_margin">Comparison of the structures of the original HTS lead and final Probe ML322. </p></div></div><p>Because several individual compounds within this [<a class="bibr" href="#ml322.r1" rid="ml322.r1">1</a>,<a class="bibr" href="#ml322.r2" rid="ml322.r2">2</a>,<a class="bibr" href="#ml322.r4" rid="ml322.r4">4</a>]triazolo[1,5-a]pyrimidin-2-yl inhibitor series showed the biochemical and cell-based potency and lack of cytotoxicity necessary to be high quality probes (<a class="figpopup" href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-figpopup="figml322t7" rid-ob="figobml322t7">Table 7</a>), we subjected samples of the top compounds to CYP450 analysis (<a class="figpopup" href="/books/NBK148493/table/ml322.t8/?report=objectonly" target="object" rid-figpopup="figml322t8" rid-ob="figobml322t8">Table 8</a>) and microsomal stability (<a class="figpopup" href="/books/NBK148493/table/ml322.t9/?report=objectonly" target="object" rid-figpopup="figml322t9" rid-ob="figobml322t9">Table 9</a>) to aid in probe selection. The results of these studies revealed that probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> does not significantly inhibit cytochrome P450 enzymes. The probe is highly stable to liver microsomes in mouse and human, with a measured half-life of 40 min and 27 min, respectively.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t8"><a href="/books/NBK148493/table/ml322.t8/?report=objectonly" target="object" title="Table 8" class="img_link icnblk_img figpopup" rid-figpopup="figml322t8" rid-ob="figobml322t8"><img class="small-thumb" src="/books/NBK148493/table/ml322.t8/?report=thumb" src-large="/books/NBK148493/table/ml322.t8/?report=previmg" alt="Table 8. Probe ML322 is not a strong inhibitor of cytochrome P450 enzymes." /></a><div class="icnblk_cntnt"><h4 id="ml322.t8"><a href="/books/NBK148493/table/ml322.t8/?report=objectonly" target="object" rid-ob="figobml322t8">Table 8</a></h4><p class="float-caption no_bottom_margin">Probe ML322 is not a strong inhibitor of cytochrome P450 enzymes. </p></div></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t9"><a href="/books/NBK148493/table/ml322.t9/?report=objectonly" target="object" title="Table 9" class="img_link icnblk_img figpopup" rid-figpopup="figml322t9" rid-ob="figobml322t9"><img class="small-thumb" src="/books/NBK148493/table/ml322.t9/?report=thumb" src-large="/books/NBK148493/table/ml322.t9/?report=previmg" alt="Table 9. Hepatic Microsome Stability of Selected HCV Core Inhibitors." /></a><div class="icnblk_cntnt"><h4 id="ml322.t9"><a href="/books/NBK148493/table/ml322.t9/?report=objectonly" target="object" rid-ob="figobml322t9">Table 9</a></h4><p class="float-caption no_bottom_margin">Hepatic Microsome Stability of Selected HCV Core Inhibitors. </p></div></div><p>Results in <a class="figpopup" href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-figpopup="figml322t7" rid-ob="figobml322t7">Tables 7</a>–<a class="figpopup" href="/books/NBK148493/table/ml322.t9/?report=objectonly" target="object" rid-figpopup="figml322t9" rid-ob="figobml322t9">9</a> show that several compounds were generally suitable as probes. We chose CID 49800087 (SR-1966 or SL274) as the probe (designated <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>) due to its superior activity in inhibiting formation of infectious HCV particles (<a class="figpopup" href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-figpopup="figml322t7" rid-ob="figobml322t7">Table 7</a>), its lack of significant inhibition of human CYPs, and the presence of a basic amine to aid solubility and ease of formulation. <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> has no serious structural liabilities. Minor issues are the relatively poor stability of <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> analogs CID 22422908 and CID 9550395 in mouse microsomes.</p><p>The probe contains a [<a class="bibr" href="#ml322.r1" rid="ml322.r1">1</a>,<a class="bibr" href="#ml322.r2" rid="ml322.r2">2</a>,<a class="bibr" href="#ml322.r4" rid="ml322.r4">4</a>]triazolo[1,5-<i>a</i>]pyrimidin-2-yl scaffold (<a class="figpopup" href="/books/NBK148493/figure/ml322.f7/?report=objectonly" target="object" rid-figpopup="figml322f7" rid-ob="figobml322f7">Figure 7</a>). It contains one amide bond which may pose a liability to protease degradation and a thioether that is potentially prone to metabolic oxidation or reduction. The probe and analogs do not react with glutathione and are chemically stable so long as they are not used in highly acidic media (pH<2). The relative ease of synthesis of the probe molecule and analogs by the described method is a strong asset for this scaffold series and this probe. Scale-up synthesis, if needed for extensive long-term biological studies, will be highly feasible.</p></div><div id="ml322.s24"><h3>3.4. SAR Tables</h3><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t13"><a href="/books/NBK148493/table/ml322.t13/?report=objectonly" target="object" title="SAR Table 1" class="img_link icnblk_img figpopup" rid-figpopup="figml322t13" rid-ob="figobml322t13"><img class="small-thumb" src="/books/NBK148493/table/ml322.t13/?report=thumb" src-large="/books/NBK148493/table/ml322.t13/?report=previmg" alt="SAR Table 1. Lead scaffolds from the HTS campaign." /></a><div class="icnblk_cntnt"><h4 id="ml322.t13"><a href="/books/NBK148493/table/ml322.t13/?report=objectonly" target="object" rid-ob="figobml322t13">SAR Table 1</a></h4><p class="float-caption no_bottom_margin">Lead scaffolds from the HTS campaign. </p></div></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t14"><a href="/books/NBK148493/table/ml322.t14/?report=objectonly" target="object" title="SAR Table 2" class="img_link icnblk_img figpopup" rid-figpopup="figml322t14" rid-ob="figobml322t14"><img class="small-thumb" src="/books/NBK148493/table/ml322.t14/?report=thumb" src-large="/books/NBK148493/table/ml322.t14/?report=previmg" alt="SAR Table 2. Probe and lead analogs." /></a><div class="icnblk_cntnt"><h4 id="ml322.t14"><a href="/books/NBK148493/table/ml322.t14/?report=objectonly" target="object" rid-ob="figobml322t14">SAR Table 2</a></h4><p class="float-caption no_bottom_margin">Probe and lead analogs. </p></div></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t15"><a href="/books/NBK148493/table/ml322.t15/?report=objectonly" target="object" title="SAR Table 3" class="img_link icnblk_img figpopup" rid-figpopup="figml322t15" rid-ob="figobml322t15"><img class="small-thumb" src="/books/NBK148493/table/ml322.t15/?report=thumb" src-large="/books/NBK148493/table/ml322.t15/?report=previmg" alt="SAR Table 3. Analogs." /></a><div class="icnblk_cntnt"><h4 id="ml322.t15"><a href="/books/NBK148493/table/ml322.t15/?report=objectonly" target="object" rid-ob="figobml322t15">SAR Table 3</a></h4><p class="float-caption no_bottom_margin">Analogs. </p></div></div></div><div id="ml322.s25"><h3>3.5. Cellular Activity</h3><div id="ml322.s26"><h4>HCV Infectivity Assays</h4><p>The next set of assays performed were biological assays used to determine the ability of compounds to inhibit production of J6/JFH1, 2a strain virus in Huh-7.5 liver cells (<a class="figpopup" href="/books/NBK148493/figure/ml322.f9/?report=objectonly" target="object" rid-figpopup="figml322f9" rid-ob="figobml322f9">Figure 9</a>). HCV infectivity is measured using real-time RT-PCR to monitor changes in expression of HCV 2a J6/JFH-1 RNA. Cells are incubated with test compound in the presence of HCV, followed by isolation of RNA, conversion to cDNA, and Taqman-based QPCR. As designed, a compound that inhibits HCV infectivity will reduce HCV RNA expression, leading to decreased production of the PCR amplicon, thereby reducing fluorescence, and increasing Ct. Compounds were tested in triplicate using a 6-point 1:10 dilution series starting at a nominal test concentration of 100 μM. Note that the TCID<sub>50</sub> values (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624575" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 624575</a>) are significantly lower than the T1/T2 QPCR values (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485271" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485271</a> Rounds 0–1; <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651574" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651574</a> (Round 2). These assays have been previously described [<a class="bibr" href="#ml322.r12" rid="ml322.r12">12</a>, <a class="bibr" href="#ml322.r17" rid="ml322.r17">17</a>].</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml322f9" co-legend-rid="figlgndml322f9"><a href="/books/NBK148493/figure/ml322.f9/?report=objectonly" target="object" title="Figure 9" class="img_link icnblk_img figpopup" rid-figpopup="figml322f9" rid-ob="figobml322f9"><img class="small-thumb" src="/books/NBK148493/bin/ml322f9.gif" src-large="/books/NBK148493/bin/ml322f9.jpg" alt="Figure 9. HCV Infectivity assay method." /></a><div class="icnblk_cntnt" id="figlgndml322f9"><h4 id="ml322.f9"><a href="/books/NBK148493/figure/ml322.f9/?report=objectonly" target="object" rid-ob="figobml322f9">Figure 9</a></h4><p class="float-caption no_bottom_margin">HCV Infectivity assay method. </p></div></div><p>Cells were plated the day before the assay. After allowing the cells to adhere overnight, test compound was prepared in HCV supernatant by making 1:10 serial dilutions from 100 μM down to 0.001 μM. Doses of test compound in virus were added to cells and incubated for 24 hours. The next day, cell culture media was removed from each well and replaced with the same dilutions of compound in complete media. This was added to cells and incubated for another 48 hours for the T1 timepoint. Next, the cells were lysed and RNA was isolated using the RNeasy kit (Qiagen, Valencia, CA). Supernatant from T1 (early stage infection) was transferred to naive cell cultures and incubated for 24 hours. After 24 hours the culture medium was removed from the cells and replaced with complete media and the cells were incubated for 48 hours for the T2 (late stage) timepoint. Cells for the T2 timepoint were lysed and RNA was isolated as was done for the T1 timepoint. cDNA was generated using the Taqman reverse transcription kit (Applied Biosystems, Foster City, CA). Quantitative real-time polymerase chain reaction (QPCR) was performed in triplicate using the LightCycler RNA Amplification Kit HybProbe master mix (Roche) with Taqman MGB Probe 6FAM-TATGAGTGTCGTGCAGCCTC-MGBNFQ on a model LightCycler480 real time PCR system (Roche). Data are expressed as the mean fold change in mRNA levels, plus or minus the SE of 3 replicates normalized to 100 μg total RNA. Primers used were forward CTTCACGCAGAAAGCGTCTA and reverse CAAGCACCCTATCAGGCAGT. The range of activity was normalized based on measurement of total RNA. Compounds with an IC50 value greater than 20 μM were considered inactive. Compounds with an IC<sub>50</sub> value equal to or less than 20 μM were considered active. All compounds tested were active in this assay. However, one compound (SR-03000001966-1/ <a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a>/ CID 49800087) exhibited significantly greater potency than all others compounds tested: TCID50 = 2 nM. As a result this compound was claimed as the probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> (<a class="figpopup" href="/books/NBK148493/table/ml322.t10/?report=objectonly" target="object" rid-figpopup="figml322t10" rid-ob="figobml322t10">Table 10</a>, next page).</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t10"><a href="/books/NBK148493/table/ml322.t10/?report=objectonly" target="object" title="Table 10" class="img_link icnblk_img figpopup" rid-figpopup="figml322t10" rid-ob="figobml322t10"><img class="small-thumb" src="/books/NBK148493/table/ml322.t10/?report=thumb" src-large="/books/NBK148493/table/ml322.t10/?report=previmg" alt="Table 10. TCID50 Results (see AID 624575)." /></a><div class="icnblk_cntnt"><h4 id="ml322.t10"><a href="/books/NBK148493/table/ml322.t10/?report=objectonly" target="object" rid-ob="figobml322t10">Table 10</a></h4><p class="float-caption no_bottom_margin">TCID<sub>50</sub> Results (see AID 624575). </p></div></div></div></div><div id="ml322.s27"><h3>3.6. Profiling Assays</h3><div id="ml322.s28"><h4>Ricerca Receptor Profiling</h4><p>The cell-based assays used in screening and in support of our lead optimization efforts define a clear mode of action for <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>: disruption of the dimerization of HCV Core proteins. To support the selectivity for this mode of action hypothesis, we submitted the probe for ligand binding competition studies against a panel of protein targets of therapeutic and/or toxicological interest including GPCRs, transporters, ion channels, and receptors. This assay was performed in duplicate by Ricerca, LLC at 10 micromolar See company website and PubChem <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624406" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 624406</a> for details.</p><p>Methods employed in this study have been adapted from the scientific literature to maximize reliability and reproducibility. Reference standards were run as an integral part of each assay to ensure the validity of the results obtained. To be considered “Active” the probe must have inhibited ligand binding to the target by greater than 50% (this value was set by Ricerca). Negative inhibition values represent a stimulation of binding. Interestingly, the top 6 targets (%INH > 40%) are restricted to the adrenergic (alpha), muscarinic (M1–M3), and dopamine transporter families. Note that the probe did not significantly inhibit the majority of targets tested (%INH < 20%). Further, the probe did not inhibit ligand binding to any target by more than 64%. While future efforts may be pursued to elucidate the mechanism by which <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> affects adrenergic (alpha), muscarinic (M1-3), and dopaminergic ligand binding, the general lack of activity in this assay and in the liver cell cytotoxicity counterscreens provides evidence for Core target specificity.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t11"><a href="/books/NBK148493/table/ml322.t11/?report=objectonly" target="object" title="Table 11" class="img_link icnblk_img figpopup" rid-figpopup="figml322t11" rid-ob="figobml322t11"><img class="small-thumb" src="/books/NBK148493/table/ml322.t11/?report=thumb" src-large="/books/NBK148493/table/ml322.t11/?report=previmg" alt="Table 11. Ricerca profiling of probe ML322: SID 103771539 /CID49800087." /></a><div class="icnblk_cntnt"><h4 id="ml322.t11"><a href="/books/NBK148493/table/ml322.t11/?report=objectonly" target="object" rid-ob="figobml322t11">Table 11</a></h4><p class="float-caption no_bottom_margin">Ricerca profiling of probe ML322: SID 103771539 /CID49800087. </p></div></div></div></div></div><div id="ml322.s29"><h2 id="_ml322_s29_">4. Discussion</h2><p>Core is a structural protein of the HCV virion and is essential for viral assembly and infectious virus production. <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> is a potent and selective inhibitor of the core protein-protein dimerization. The probe has an IC<sub>50</sub> of 8.4 μM in the absorbance-based biochemical Amplified Luminescent Proximity Homogeneous Assay (Alphascreen), indicating it can block the interaction between HCV core proteins. Because core-core dimerization is thought to be important for mediating viral assembly and formation of the viral nucleocapsid cell-based infectivity assays were performed to determine whether the probe could block infectivity using a cell culture system. The probe exhibits an IC<sub>50</sub> of 1.8 μM (T2 QPCR) for blocking the levels of total HCV RNA in cells. In addition the probe potently reduces the levels of infections HCV RNA in cells (TCID<sub>50</sub> = 2 nM). The significantly lower IC<sub>50</sub> value in the TCID<sub>50</sub> assay, relative to the T2 QPCR assay, reflects the fact that one needs to interfere with only a few of the potential core-core protein interactions to prevent formation an infectious HCV particle (TCID<sub>50</sub> assay), whereas the T2 QPCR data reflects the concentration required to inhibit one-half of all possible core-core dimer interactions in the <i>in vivo</i> experiment. All sets of biochemical and cellular assay data were highly reproducible between batches and runs. Importantly, <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> also consistently lacks activity in the hepatocyte cytotoxicity counterscreen (CC<sub>50</sub> >30 μM), demonstrating that it does not significantly affect liver cell viability. Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> lacks significant inhibitory activity effects against the majority of therapeutic targets tested (Ricerca screening). Together these results demonstrate that probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> far surpasses the probe criteria set forth in the chemical probe development plan.</p><p><i>It must be noted that protein-protein interactions are notoriously difficult to efficiently and selectivity disrupt using small molecule ligands and that micromolar activity for disrupting a protein-protein interaction using a rule-of-five compliant small molecule is noteworthy.</i> In fact, certain clinically useful anticancer compounds acting by disrupting protein-protein interactions are less potent against their targeted proteins [<a class="bibr" href="#ml322.r18" rid="ml322.r18">18</a>]. The finding that probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> is not cytotoxic to liver cells (Huh7.5) indicates that the probe may be particularly useful to researchers in testing the hypothesis that targeting interactions of core proteins can thwart mechanisms used by HCV to avoid inhibition by other immune enhancing drugs such as pegylated interferon or ribavirin, treatments currently in use for hepatitis with significant off-target effects.</p><p>The probe lacks general reactivity (is nonreactive with glutathione), is not a significant inhibitor of major CYP450 isoforms, and is readily synthesized in high purity without chromatography in a single chemical step. Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> also has chemical stability, with useful levels of water solubility (>100 μM in PBS, >100 μM in simulated assay conditions). Moreover, the probe has favorable properties associated with drug-likeness, with a moderate molecular weight (454), acceptable parameters for LogP (2.76), tPSA (84.7), and lack of groups associated with inherent toxicity. Due to these chemical and biological properties, <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> should prove useful in <i>in vitro</i> and perhaps in <i>in vivo</i> studies aimed at understanding how HCV core dimerization regulates HCV packaging, host cell oncogenesis, and suppression of host T cell responses.</p><div id="ml322.s30"><h3>4.1. Comparison to existing art and how the new probe is an improvement</h3><p>At the onset of the HTS campaign no published prior art small molecules were known to selectively disrupt the dimerization of HCV core. However, during the HTS and chemistry effort that led to discovery of <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>, the assay provider in collaboration with Boston University discovered several molecules with activity in the HCV Core dimerization Alphascreen and cell-based infectivity assays (<a class="figpopup" href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object" rid-figpopup="figml322t7" rid-ob="figobml322t7">Table 7</a>). While these compounds represent exciting and promising scaffolds, none exhibits the cell-based potency as high as that of <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> (TCID50 = 2nM). Additional studies are needed to elucidate the specific mechanisms by which these compounds inhibit core dimerization. However, 4 of the 5 compounds in <a class="figpopup" href="/books/NBK148493/table/ml322.t12/?report=objectonly" target="object" rid-figpopup="figml322t12" rid-ob="figobml322t12">Table 12</a> (<a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> included) do not exhibit liver cell toxicity, suggesting their action is targeted to the viral core protein.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml322t12"><a href="/books/NBK148493/table/ml322.t12/?report=objectonly" target="object" title="Table 12" class="img_link icnblk_img figpopup" rid-figpopup="figml322t12" rid-ob="figobml322t12"><img class="small-thumb" src="/books/NBK148493/table/ml322.t12/?report=thumb" src-large="/books/NBK148493/table/ml322.t12/?report=previmg" alt="Table 12. Comparison of the prior art with HCV Core probe ML322." /></a><div class="icnblk_cntnt"><h4 id="ml322.t12"><a href="/books/NBK148493/table/ml322.t12/?report=objectonly" target="object" rid-ob="figobml322t12">Table 12</a></h4><p class="float-caption no_bottom_margin">Comparison of the prior art with HCV Core probe ML322. </p></div></div></div><div id="ml322.s31"><h3>4.2. Mechanism of Action Studies</h3><p>Because our screening and hit follow-up protocols include relevant targets and anti-targets in both biochemical assays and cell-based assays, we were able to establish a HCV core dimerization specific mode of action. <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> has robust on-target activity in biochemical and cell-based assays and lacks toxicity in the same Huh7.5 liver cell line used for the cell-based infectivity assays. It has clear advantages over prior art in selectivity and defined mode of action (<a class="figpopup" href="/books/NBK148493/table/ml322.t12/?report=objectonly" target="object" rid-figpopup="figml322t12" rid-ob="figobml322t12">Table 12</a>).</p></div><div id="ml322.s32"><h3>4.3. Planned Future Studies</h3><p>Sadly, the assay provider Dr. Donny Strosberg suddenly passed away during the probe development effort. As a result there are no further efforts planned by the SRIMSC for this probe. However, the compound and analogs have been made available to the MLSMR for other researchers to use as tools for inhibiting HCV core dimerization and HCV infectivity in cells.</p></div></div><div id="ml322.s33"><h2 id="_ml322_s33_">5. References</h2><dl class="temp-labeled-list"><dl class="bkr_refwrap"><dt>1.</dt><dd><div class="bk_ref" id="ml322.r1">Hoofnagle JH. Course and outcome of hepatitis C. <span><span class="ref-journal">Hepatology. </span>2002;<span class="ref-vol">36</span>(5 Suppl 1):s21–s29.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/12407573" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 12407573</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>2.</dt><dd><div class="bk_ref" id="ml322.r2">Soriano V, et al. Challenges and opportunities for hepatitis C drug development in HIV-hepatitis C virus-co-infected patients. <span><span class="ref-journal">AIDS. </span>2011;<span class="ref-vol">25</span>(18):2197–208.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/21866039" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 21866039</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>3.</dt><dd><div class="bk_ref" id="ml322.r3">Dore GJ. The changing therapeutic landscape for hepatitis C. <span><span class="ref-journal">Med J Aust. </span>2012;<span class="ref-vol">196</span>(10):629–32.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/22676877" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 22676877</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>4.</dt><dd><div class="bk_ref" id="ml322.r4">Hoofnagle JH. Course and outcome of hepatitis C. <span><span class="ref-journal">hepatology. </span>2002;<span class="ref-vol">36</span>(5 Suppl 1):S21–9.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/12407573" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 12407573</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>5.</dt><dd><div class="bk_ref" id="ml322.r5">Lin C, et al. Processing in the hepatitis C virus E2-NS2 region: identification of p7 and two distinct E2-specific products with different C termini. <span><span class="ref-journal">J Virol. </span>1994;<span class="ref-vol">68</span>(8):5063–73.</span> [<a href="/pmc/articles/PMC236449/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC236449</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/7518529" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 7518529</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>6.</dt><dd><div class="bk_ref" id="ml322.r6">Moradpour D, Blum HE. A primer on the molecular virology of hepatitis C. <span><span class="ref-journal">Liver Int. </span>2004;<span class="ref-vol">24</span>(6):519–25.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/15566499" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 15566499</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>7.</dt><dd><div class="bk_ref" id="ml322.r7">Majeau N, et al. The N-terminal half of the core protein of hepatitis C virus is sufficient for nucleocapsid formation. <span><span class="ref-journal">J Gen Virol. </span>2004;<span class="ref-vol">85</span>(Pt 4):971–81.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/15039539" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 15039539</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>8.</dt><dd><div class="bk_ref" id="ml322.r8">Brothers SP, et al. Selective and brain penetrant neuropeptide y y2 receptor antagonists discovered by whole-cell high-throughput screening. <span><span class="ref-journal">Mol Pharmacol. </span>2010;<span class="ref-vol">77</span>(1):46–57.</span> [<a href="/pmc/articles/PMC2802430/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2802430</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/19837904" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 19837904</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>9.</dt><dd><div class="bk_ref" id="ml322.r9">Terent’ev AO, et al. Facile and selective procedure for the synthesis of bridged 1,2,4,5-tetraoxanes; strong acids as cosolvents and catalysts for addition of hydrogen peroxide to beta-diketones. <span><span class="ref-journal">J Org Chem. </span>2009;<span class="ref-vol">74</span>(9):3335–40.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/19298073" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 19298073</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>10.</dt><dd><div class="bk_ref" id="ml322.r10">Sakai T, et al. One-Pot C-Arylmethylation of Active Methylene Compounds with Aromatic Aldehydes Induced by a Me3SiCl–NaI–MeCN Reagent. <span><span class="ref-journal">Bull. Chem. Soc. Jpn. </span>1989;<span class="ref-vol">62</span>(12):4072–4074.</span></div></dd></dl><dl class="bkr_refwrap"><dt>11.</dt><dd><div class="bk_ref" id="ml322.r11">Chen Q, et al. Synthesis, antifungal activity and CoMFA analysis of novel 1,2,4-triazolo[1,5-a]pyrimidine derivatives. <span><span class="ref-journal">Eur J Med Chem. </span>2008;<span class="ref-vol">43</span>(3):595–603.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/17618711" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 17618711</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>12.</dt><dd><div class="bk_ref" id="ml322.r12">Wei W, et al. New small molecule inhibitors of hepatitis C virus. <span><span class="ref-journal">Bioorg Med Chem Lett. </span>2009;<span class="ref-vol">19</span>(24):6926–30.</span> [<a href="/pmc/articles/PMC2783740/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2783740</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/19896376" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 19896376</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>13.</dt><dd><div class="bk_ref" id="ml322.r13">Lipinski CA, et al. Experimental and computational approaches to estimate solubility and permeability in drug discovery and development settings. <span><span class="ref-journal">Adv Drug Deliv Rev. </span>2001;<span class="ref-vol">46</span>(1–3):3–26.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/11259830" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 11259830</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>14.</dt><dd><div class="bk_ref" id="ml322.r14">Walters WP. Going further than Lipinski's rule in drug design. <span><span class="ref-journal">Expert Opin Drug Discov. </span>2012;<span class="ref-vol">7</span>(2):99–107.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/22468912" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 22468912</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>15.</dt><dd><div class="bk_ref" id="ml322.r15">Rishton GM. Nonleadlikeness and leadlikeness in biochemical screening. <span><span class="ref-journal">Drug Discov Today. </span>2003;<span class="ref-vol">8</span>(2):86–96.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/12565011" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 12565011</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>16.</dt><dd><div class="bk_ref" id="ml322.r16">Benigni R, Bossa C. Mechanisms of chemical carcinogenicity and mutagenicity: a review with implications for predictive toxicology. <span><span class="ref-journal">Chem Rev. </span>2011;<span class="ref-vol">111</span>(4):2507–36.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/21265518" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 21265518</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>17.</dt><dd><div class="bk_ref" id="ml322.r17">Kota S, et al. A time-resolved fluorescence-resonance energy transfer assay for identifying inhibitors of hepatitis C virus core dimerization. <span><span class="ref-journal">Assay Drug Dev Technol. </span>2010;<span class="ref-vol">8</span>(1):96–105.</span> [<a href="/pmc/articles/PMC2971647/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2971647</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/20035614" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 20035614</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>18.</dt><dd><div class="bk_ref" id="ml322.r18">Verdine GL, Walensky LD. The challenge of drugging undruggable targets in cancer: lessons learned from targeting BCL-2 family members. <span><span class="ref-journal">Clin Cancer Res. </span>2007;<span class="ref-vol">13</span>(24):7264–70.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/18094406" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 18094406</span></a>]</div></dd></dl></dl></div><div id="bk_toc_contnr"></div></div></div><div class="fm-sec"><h2 id="_NBK148493_pubdet_">Publication Details</h2><h3>Author Information and Affiliations</h3><p class="contrib-group"><h4>Authors</h4><span itemprop="author">Smitha Kota</span>,<sup>1</sup> <span itemprop="author">Franck Madoux</span>,<sup>2</sup> <span itemprop="author">Peter Chase</span>,<sup>2</sup> <span itemprop="author">Virginia Takahashi</span>,<sup>1</sup> <span itemprop="author">Qin Liu</span>,<sup>3</sup> <span itemprop="author">Becky A. Mercer</span>,<sup>2</sup> <span itemprop="author">Michael Cameron</span>,<sup>4</sup> <span itemprop="author">A. Donny Strosberg</span>,<sup>1</sup> <span itemprop="author">William Roush</span>,<sup>3</sup> and <span itemprop="author">Peter Hodder</span><sup>2,4</sup><sup>,5</sup>.</p><h4>Affiliations</h4><div class="affiliation"><sup>1</sup>
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The Scripps Research Institute, Department of Infectology, Translational Research Institute Jupiter, FL</div><div class="affiliation"><sup>2</sup>
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The Scripps Research Institute, Lead Identification Division, Jupiter, FL</div><div class="affiliation"><sup>3</sup>
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The Scripps Research Institute, Department of Chemistry, Jupiter, FL</div><div class="affiliation"><sup>4</sup>
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The Scripps Research Institute, Department of Molecular Therapeutics, Jupiter, FL</div><div class="affiliation">
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<sup>5</sup> Corresponding author:
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<span class="before-email-separator"></span><span class="email-label">Email: </span><a href="mailto:dev@null" data-email="ude.sppircs@preddoh" class="oemail">ude.sppircs@preddoh</a></div><h3>Publication History</h3><p class="small">Received: <span itemprop="datePublished">December 17, 2012</span>; Last Update: <span itemprop="dateModified">May 13, 2014</span>.</p><h3>Copyright</h3><div><div class="half_rhythm"><a href="/books/about/copyright/">Copyright Notice</a></div></div><h3>Publisher</h3><p>National Center for Biotechnology Information (US), Bethesda (MD)</p><h3>NLM Citation</h3><p>Kota S, Madoux F, Chase P, et al. ML322, A Small Molecule Inhibitor of Dimerization of the Core Protein of Hepatitis C Virus (HCV) 2012 Dec 17 [Updated 2014 May 13]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-. <span class="bk_cite_avail"></span></p></div><div class="small-screen-prev"><a href="/books/n/mlprobe/ml324/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M75,30 c-80,60 -80,0 0,60 c-30,-60 -30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Prev</text></svg></a></div><div class="small-screen-next"><a href="/books/n/mlprobe/ml321/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M25,30c80,60 80,0 0,60 c30,-60 30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Next</text></svg></a></div></article><article data-type="fig" id="figobml322fu1"><div id="ml322.fu1" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK148493/bin/ml322fu1.jpg" alt="Probe ML322." /></div><h3><span class="title">Probe ML322</span></h3></div></article><article data-type="table-wrap" id="figobml322tu1"><div id="ml322.tu1" class="table"><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.tu1/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.tu1_lrgtbl__"><table class="no_bottom_margin"><thead><tr><th id="hd_h_ml322.tu1_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID<br />ML#<br />SIDs</th><th id="hd_h_ml322.tu1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Target Name</th><th id="hd_h_ml322.tu1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">IC<sub>50</sub> [SID, AID]</th><th id="hd_h_ml322.tu1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Anti-Target</th><th id="hd_h_ml322.tu1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">IC<sub>50</sub> [SID, AID]</th><th id="hd_h_ml322.tu1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Fold Selective<sup>†</sup></th><th id="hd_h_ml322.tu1_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Additional Assay(s) Name:<br />[SID, AID]</th></tr></thead><tbody><tr><td headers="hd_h_ml322.tu1_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID 49800087<br /><a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>; SR-03000001966<br /><br /><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a> (synth) Probe;<br /><br /><a href="https://pubchem.ncbi.nlm.nih.gov/substance/125311267" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 125311267</a> (re-synth)</td><td headers="hd_h_ml322.tu1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HCV Core Infectivity</td><td headers="hd_h_ml322.tu1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>TCID</b><sub><b>50</b></sub>
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<b>= 2 nM</b><br />[<a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651575" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651575</a>]</td><td headers="hd_h_ml322.tu1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Liver Cell Cytotoxicity</td><td headers="hd_h_ml322.tu1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>CC</b><sub><b>50</b></sub>
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<b>=33.79 μM</b><br />[<a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651583" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651583</a>]</td><td headers="hd_h_ml322.tu1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>16,000-fold</td><td headers="hd_h_ml322.tu1_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>T1/T2 QPCR IC</b><sub><b>50</b></sub>
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<b>=1.8 μM</b><br />[<a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651574" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651574</a>]<br /><br /><b>IC</b><sub><b>50</b></sub>
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<b>= 8.4 μM</b><br />[<a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651577" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651577</a>]<br />(Alphascreen)<br /><br /><b>Ricerca Selectivity Profiling</b> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 103771539</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624406" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 624406</a>]<br /><u>Inactive</u> at 63 Receptors, Transporters, and Channels.<br /><u>Active</u> at 4: Muscarinic M3, Adrenergic a1D & a1B, and Dopamine Transporter (DAT).</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>†</dt><dd><div id="ml322.tfn1"><p class="no_margin">Fold-selectivity was calculated as IC<sub>50</sub> for anti-target/IC<sub>50</sub> for the target, both cell-based assays</p></div></dd></dl></dl></div></div></div></article><article data-type="table-wrap" id="figobml322t1"><div id="ml322.t1" class="table"><h3><span class="label">Table 1</span><span class="title">HCV Core Inhibitors Project (see also <u>Summary AID 1911</u>)</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t1/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t1_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Stage</th><th id="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Assay Name</th><th id="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Tested Substances</th><th id="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active Substances</th><th id="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">PubChem AID</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t1_1_1_1_1" rowspan="3" colspan="1" style="text-align:center;vertical-align:middle;"><b>HTS</b> (run by Scripps: MLSMR Liquids)</td><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Primary HTS Assay (TRFRET 1X%INH)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">302,755</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">998</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/1899" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">1899</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Confirmation HTS Assay (TRFRET 3X%INH)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2,251</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2152" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2152</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HTS Dose Response (TRFRET 3XIC50)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">92</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2159" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2159</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>SAR</b> (run by Scripps: powders)</td><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Dose Response (TRFRET 3XIC50)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2488" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2488</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>SAR</b> (outsourced)</td><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Ricerca Profiling</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624406" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">624406</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_1" rowspan="3" colspan="1" style="text-align:center;vertical-align:middle;"><b>SAR</b> (run by assay provider: powders)<br /><b>Rounds 0–1</b></td><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Core AlphaScreen</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/463085" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">463085</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HCV Infectivity (QPCR)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485271" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485271</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HUH7.5 Cytotoxicity</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485280" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485280</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_1" rowspan="5" colspan="1" style="text-align:center;vertical-align:middle;"><b>SAR</b> (run by assay provider: powders)<br /><b>Rounds 1–2</b></td><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Core AlphaScreen (%INH)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">82</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">41</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651576" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">651576</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Core AlphaScreen (IC50)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">15</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">12</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651577" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">651577</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HCV Infectivity (T1/T2 QPCR)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">12</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651574" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">651574</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HUH7.5 Cytotoxicity</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651583" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">651583</a></td></tr><tr><td headers="hd_h_ml322.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HCV TCID<sub>50</sub> (QPCR)</td><td headers="hd_h_ml322.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9</td><td headers="hd_h_ml322.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9</td><td headers="hd_h_ml322.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624575" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">624575</a></td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml322f1"><div id="ml322.f1" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK148493/bin/ml322f1.jpg" alt="Figure 1. Alphascreen Technology." /></div><h3><span class="label">Figure 1</span><span class="title">Alphascreen Technology</span></h3></div></article><article data-type="table-wrap" id="figobml322t2"><div id="ml322.t2" class="table"><h3><span class="label">Table 2</span><span class="title">Probe Properties</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t2/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t2_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t2_1_1_1_1" colspan="4" rowspan="1" style="text-align:center;vertical-align:middle;">Compound Information</th><th id="hd_h_ml322.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Solubility in PBS</th><th id="hd_h_ml322.t2_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Solubility in Media + FBS</th><th id="hd_h_ml322.t2_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Chemical Stability 6Hr in 50 μM GSH</th><th id="hd_h_ml322.t2_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Stability in PBS</th></tr><tr><th headers="hd_h_ml322.t2_1_1_1_1" id="hd_h_ml322.t2_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Compound</th><th headers="hd_h_ml322.t2_1_1_1_1" id="hd_h_ml322.t2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th headers="hd_h_ml322.t2_1_1_1_1" id="hd_h_ml322.t2_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th headers="hd_h_ml322.t2_1_1_1_1" id="hd_h_ml322.t2_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th headers="hd_h_ml322.t2_1_1_1_2" id="hd_h_ml322.t2_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">(μM)</th><th headers="hd_h_ml322.t2_1_1_1_3" id="hd_h_ml322.t2_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">(μM)</th><th headers="hd_h_ml322.t2_1_1_1_4" id="hd_h_ml322.t2_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Stable-Yes/No</th><th headers="hd_h_ml322.t2_1_1_1_5" id="hd_h_ml322.t2_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">t1/2 (hr)</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a></td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000001966</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49800087</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771539</a></td><td headers="hd_h_ml322.t2_1_1_1_2 hd_h_ml322.t2_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>≥100</i></b></td><td headers="hd_h_ml322.t2_1_1_1_3 hd_h_ml322.t2_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>≥100</i></b></td><td headers="hd_h_ml322.t2_1_1_1_4 hd_h_ml322.t2_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Yes</td><td headers="hd_h_ml322.t2_1_1_1_5 hd_h_ml322.t2_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 48 hr</td></tr><tr><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Analog 1</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000001539</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">22422908</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022352" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022352</a></td><td headers="hd_h_ml322.t2_1_1_1_2 hd_h_ml322.t2_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>≥</b><b><i>100</i></b></td><td headers="hd_h_ml322.t2_1_1_1_3 hd_h_ml322.t2_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>≥100</i></b></td><td headers="hd_h_ml322.t2_1_1_1_4 hd_h_ml322.t2_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Yes</td><td headers="hd_h_ml322.t2_1_1_1_5 hd_h_ml322.t2_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 48 hr</td></tr><tr><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Analog 2</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-01000668805</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9550395</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771538" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771538</a></td><td headers="hd_h_ml322.t2_1_1_1_2 hd_h_ml322.t2_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>63.6</i></b></td><td headers="hd_h_ml322.t2_1_1_1_3 hd_h_ml322.t2_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>81.4</i></b></td><td headers="hd_h_ml322.t2_1_1_1_4 hd_h_ml322.t2_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Yes</td><td headers="hd_h_ml322.t2_1_1_1_5 hd_h_ml322.t2_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 48 hr</td></tr><tr><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Analog 3</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002010</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49849980</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/125311268" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">125311268</a></td><td headers="hd_h_ml322.t2_1_1_1_2 hd_h_ml322.t2_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>64.1</i></b></td><td headers="hd_h_ml322.t2_1_1_1_3 hd_h_ml322.t2_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>79.9</i></b></td><td headers="hd_h_ml322.t2_1_1_1_4 hd_h_ml322.t2_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Yes</td><td headers="hd_h_ml322.t2_1_1_1_5 hd_h_ml322.t2_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 48 hr</td></tr><tr><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Analog 4</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002015</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49849986</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179728" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179728</a></td><td headers="hd_h_ml322.t2_1_1_1_2 hd_h_ml322.t2_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>63.7</i></b></td><td headers="hd_h_ml322.t2_1_1_1_3 hd_h_ml322.t2_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>68.4</i></b></td><td headers="hd_h_ml322.t2_1_1_1_4 hd_h_ml322.t2_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Yes</td><td headers="hd_h_ml322.t2_1_1_1_5 hd_h_ml322.t2_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 48 hr</td></tr><tr><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Analog 5</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002037</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49852628</td><td headers="hd_h_ml322.t2_1_1_1_1 hd_h_ml322.t2_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/125311269" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">125311269</a></td><td headers="hd_h_ml322.t2_1_1_1_2 hd_h_ml322.t2_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>18.2</i></b></td><td headers="hd_h_ml322.t2_1_1_1_3 hd_h_ml322.t2_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b><i>54.3</i></b></td><td headers="hd_h_ml322.t2_1_1_1_4 hd_h_ml322.t2_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Yes</td><td headers="hd_h_ml322.t2_1_1_1_5 hd_h_ml322.t2_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 48 hr</td></tr></tbody></table><table><thead><tr><th id="hd_h_ml322.t2_1_2_1_1" colspan="3" rowspan="1" style="text-align:left;vertical-align:top;">Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> Stability in PBS Buffer (pH 7.4)<br />Sample concentration: 10 μM<br />Storage condition: Ambient Temperature</th></tr><tr><th headers="hd_h_ml322.t2_1_2_1_1" id="hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Time (hr)</th><th headers="hd_h_ml322.t2_1_2_1_1" id="hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">% remaining</th><th headers="hd_h_ml322.t2_1_2_1_1" id="hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">ln(%remaining)</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">100.0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.61</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.5</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">100.0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.61</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9.2</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">100.0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.61</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">13.8</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">99.0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.60</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">16.2</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">99.0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.60</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">25</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">101.0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.62</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">48</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">100.0</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.61</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">half-life</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−ln2/slope</td><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 48 hr</td></tr><tr><td headers="hd_h_ml322.t2_1_2_1_1 hd_h_ml322.t2_1_2_2_1 hd_h_ml322.t2_1_2_2_2 hd_h_ml322.t2_1_2_2_3" colspan="3" rowspan="1" style="text-align:center;vertical-align:top;"><b>slope =</b> 0.0001</td></tr></tbody></table></div><div class="graphic"><img src="/books/NBK148493/bin/ml322fu2.jpg" alt="Image ml322fu2" /></div></div></article><article data-type="table-wrap" id="figobml322t3"><div id="ml322.t3" class="table"><h3><span class="label">Table 3</span><span class="title">Probe and Analogs submitted to the MLSMR</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t3/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t3_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t3_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Designation</th><th id="hd_h_ml322.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t3_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR ID</th><th id="hd_h_ml322.t3_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS #</th><th id="hd_h_ml322.t3_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Amount submitted (mg)</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t3_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> (re-synth)</b></td><td headers="hd_h_ml322.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu3.jpg" alt="Image ml322fu3.jpg" /></div></td><td headers="hd_h_ml322.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800087</td><td headers="hd_h_ml322.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771539</a></td><td headers="hd_h_ml322.t3_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001966-1</td><td headers="hd_h_ml322.t3_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS004173141</td><td headers="hd_h_ml322.t3_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">16 mg</td></tr><tr><td headers="hd_h_ml322.t3_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>Analog 1 (HTS hit)</b></td><td headers="hd_h_ml322.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu4.jpg" alt="Image ml322fu4.jpg" /></div></td><td headers="hd_h_ml322.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422908</td><td headers="hd_h_ml322.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022352" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022352</a></td><td headers="hd_h_ml322.t3_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001539-1</td><td headers="hd_h_ml322.t3_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS004173139</td><td headers="hd_h_ml322.t3_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">16.1 mg</td></tr><tr><td headers="hd_h_ml322.t3_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>Analog 2</b></td><td headers="hd_h_ml322.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu5.jpg" alt="Image ml322fu5.jpg" /></div></td><td headers="hd_h_ml322.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9550395</td><td headers="hd_h_ml322.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771538" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771538</a></td><td headers="hd_h_ml322.t3_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000668805-2</td><td headers="hd_h_ml322.t3_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS004173140</td><td headers="hd_h_ml322.t3_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20.2 mg</td></tr><tr><td headers="hd_h_ml322.t3_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>Analog 3</b></td><td headers="hd_h_ml322.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu6.jpg" alt="Image ml322fu6.jpg" /></div></td><td headers="hd_h_ml322.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849980</td><td headers="hd_h_ml322.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/125311268" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">125311268</a></td><td headers="hd_h_ml322.t3_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002010-2</td><td headers="hd_h_ml322.t3_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS004173142</td><td headers="hd_h_ml322.t3_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">14 mg</td></tr><tr><td headers="hd_h_ml322.t3_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>Analog 4</b></td><td headers="hd_h_ml322.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu7.jpg" alt="Image ml322fu7.jpg" /></div></td><td headers="hd_h_ml322.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849986</td><td headers="hd_h_ml322.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179728" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179728</a></td><td headers="hd_h_ml322.t3_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002015-1</td><td headers="hd_h_ml322.t3_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS004173143</td><td headers="hd_h_ml322.t3_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">32 mg</td></tr><tr><td headers="hd_h_ml322.t3_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>Analog 5</b></td><td headers="hd_h_ml322.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu8.jpg" alt="Image ml322fu8.jpg" /></div></td><td headers="hd_h_ml322.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852628</td><td headers="hd_h_ml322.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/125311269" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">125311269</a></td><td headers="hd_h_ml322.t3_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002037-2</td><td headers="hd_h_ml322.t3_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS004173144</td><td headers="hd_h_ml322.t3_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">15.9 mg</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml322f2"><div id="ml322.f2" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%202.%20Synthesis%20of%20probe%20ML322.&p=BOOKS&id=148493_ml322f2.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img data-src="/books/NBK148493/bin/ml322f2.jpg" alt="Figure 2. Synthesis of probe ML322." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 2</span><span class="title">Synthesis of probe ML322</span></h3></div></article><article data-type="fig" id="figobml322f3"><div id="ml322.f3" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%203.%20HCV%20Core%20dimerization%20HTS%20campaign%20overview.&p=BOOKS&id=148493_ml322f3.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img data-src="/books/NBK148493/bin/ml322f3.jpg" alt="Figure 3. HCV Core dimerization HTS campaign overview." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 3</span><span class="title">HCV Core dimerization HTS campaign overview</span></h3></div></article><article data-type="table-wrap" id="figobml322t4"><div id="ml322.t4" class="table"><h3><span class="label">Table 4</span><span class="title">Most potent scaffolds from the HTS campaign</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t4/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t4_lrgtbl__"><table class="no_margin"><thead><tr><th id="hd_h_ml322.t4_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th id="hd_h_ml322.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t4_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2159" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2159</a> HTRF IC50 (uM)</th><th id="hd_h_ml322.t4_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome<sup>*</sup></th></tr></thead><tbody><tr><td headers="hd_h_ml322.t4_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000812946-1</td><td headers="hd_h_ml322.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/49646637" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">49646637</a></td><td headers="hd_h_ml322.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1557339</td><td headers="hd_h_ml322.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu9.jpg" alt="Image ml322fu9.jpg" /></div></td><td headers="hd_h_ml322.t4_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4.78</td><td headers="hd_h_ml322.t4_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t4_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000819396-1</td><td headers="hd_h_ml322.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/49716510" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">49716510</a></td><td headers="hd_h_ml322.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422906</td><td headers="hd_h_ml322.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu10.jpg" alt="Image ml322fu10.jpg" /></div></td><td headers="hd_h_ml322.t4_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.87</td><td headers="hd_h_ml322.t4_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t4_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000839920-1</td><td headers="hd_h_ml322.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/56317368" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">56317368</a></td><td headers="hd_h_ml322.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24979772</td><td headers="hd_h_ml322.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu11.jpg" alt="Image ml322fu11.jpg" /></div></td><td headers="hd_h_ml322.t4_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">16.73</td><td headers="hd_h_ml322.t4_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t4_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000839796-1</td><td headers="hd_h_ml322.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/56320113" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">56320113</a></td><td headers="hd_h_ml322.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24981182</td><td headers="hd_h_ml322.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu12.jpg" alt="Image ml322fu12.jpg" /></div></td><td headers="hd_h_ml322.t4_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">18.22</td><td headers="hd_h_ml322.t4_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t4_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000763803-1</td><td headers="hd_h_ml322.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/47193664" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">47193664</a></td><td headers="hd_h_ml322.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5280489</td><td headers="hd_h_ml322.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu13.jpg" alt="Image ml322fu13.jpg" /></div></td><td headers="hd_h_ml322.t4_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">29.69</td><td headers="hd_h_ml322.t4_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>*</dt><dd><div id="ml322.tfn2"><p class="no_margin">An IC50 value less than 10 micromolar is the numeric activity cutoff for HTS assays.</p></div></dd></dl></dl></div></div></div></article><article data-type="fig" id="figobml322f6"><div id="ml322.f6" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK148493/bin/ml322f6.jpg" alt="Figure 6. Structures of prior art compounds SL201 and SL209." /></div><h3><span class="label">Figure 6</span><span class="title">Structures of prior art compounds SL201 and SL209</span></h3></div></article><article data-type="table-wrap" id="figobml322t5"><div id="ml322.t5" class="table"><h3><span class="label">Table 5</span><span class="title">The top Round 0 analog results</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t5/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t5_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR number</th><th id="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Vendor</th><th id="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Late stage (SRIMSC) <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2488" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2488</a> HTRF Assay (IC50)</th><th id="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485280" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485280</a> Cytox CC50</th><th id="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t5_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/463085" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 463085</a> Alphascreen IC50 (uM)</th><th id="hd_h_ml322.t5_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t5_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485271" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485271</a> HCV QPCR T1 IC50 (uM)</th><th id="hd_h_ml322.t5_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T2 IC50 (uM)</th><th id="hd_h_ml322.t5_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000819396-2</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu14.jpg" alt="Image ml322fu14.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219211" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219211</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422906</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.666</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">123</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5</td><td headers="hd_h_ml322.t5_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t5_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">6.6</td><td headers="hd_h_ml322.t5_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">12</td><td headers="hd_h_ml322.t5_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000839920-2</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu15.jpg" alt="Image ml322fu15.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219213" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219213</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24979772</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20.49</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">140.7</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">29.8</td><td headers="hd_h_ml322.t5_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_12 hd_h_ml322.t5_1_1_1_13 hd_h_ml322.t5_1_1_1_14" colspan="3" rowspan="8" style="text-align:center;vertical-align:middle;">Not tested due to lack of HCV Core activity in FRET assay</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000763803-4</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu16.jpg" alt="Image ml322fu16.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219208" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219208</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5280489</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Sigma Chemical</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">171.9</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">34.8</td><td headers="hd_h_ml322.t5_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000000036-3</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu17.jpg" alt="Image ml322fu17.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219207" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219207</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1780</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Sigma Chemical</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">178.6</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_10 hd_h_ml322.t5_1_1_1_11" colspan="2" rowspan="6" style="text-align:center;vertical-align:middle;">Not tested due to lack of HCV Core activity in FRET assay</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000812654-2</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu18.jpg" alt="Image ml322fu18.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219209" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219209</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">694792</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemBridge</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">142.9</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000812946-2</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu19.jpg" alt="Image ml322fu19.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219210" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219210</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1557339</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Pharmeks</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46.17</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24.7</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000839796-2</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu20.jpg" alt="Image ml322fu20.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219212" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219212</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24981182</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">27.4</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000840236-2</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu21.jpg" alt="Image ml322fu21.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219214" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219214</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24979394</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">40.43</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">148.4</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000849831-2</td><td headers="hd_h_ml322.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu22.jpg" alt="Image ml322fu22.jpg" /></div></td><td headers="hd_h_ml322.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219215" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219215</a></td><td headers="hd_h_ml322.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9426403</td><td headers="hd_h_ml322.t5_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t5_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">21.17</td><td headers="hd_h_ml322.t5_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t5_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">163.3</td><td headers="hd_h_ml322.t5_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml322t6"><div id="ml322.t6" class="table"><h3><span class="label">Table 6</span><span class="title">Round 1 Analogs: Alphascreen results</span></h3><div class="caption"><p>SL274 was ultimately claimed as probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>.</p></div><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t6/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t6_lrgtbl__"><table><thead><tr><th id="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL</th><th id="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th id="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Vendor</th><th id="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">%INH Core Dimer at 15 μM (Alpha)</th><th id="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Core Dimer IC<sub>50</sub> (μM)</th><th id="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL270</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000668805-2</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu23.jpg" alt="Image ml322fu23.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771538" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771538</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9550395</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">60</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL282</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002013-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu24.jpg" alt="Image ml322fu24.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179726" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179726</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849987</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">87</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.3</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL298</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002055-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu25.jpg" alt="Image ml322fu25.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233655" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233655</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859547</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">83</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.6</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"></td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001539-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="2" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu26.jpg" alt="Image ml322fu26.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022352" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022352</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="2" colspan="1" style="text-align:center;vertical-align:middle;">22422908</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">97</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.9</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL242R</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001539-3</td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99309211" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99309211</a></td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">82</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.9</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL293</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002037-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu27.jpg" alt="Image ml322fu27.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223097" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223097</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852628</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">79</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.6</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL279</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002010-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu28.jpg" alt="Image ml322fu28.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179723" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179723</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849980</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">66</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL297</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002054-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu29.jpg" alt="Image ml322fu29.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233654" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233654</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859550</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.1</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL284</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002015-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu30.jpg" alt="Image ml322fu30.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179728" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179728</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849986</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">65</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.7</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL274</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001966-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu31.jpg" alt="Image ml322fu31.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771539</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800087</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">51</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">8.4</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL415</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002229-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu32.jpg" alt="Image ml322fu32.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050594" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050594</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914829</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">85</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.9</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL296</td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002053-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu33.jpg" alt="Image ml322fu33.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233653" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233653</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859551</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">62</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10.2</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"></td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001967-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu34.jpg" alt="Image ml322fu34.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771540" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771540</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800088</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">56</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">18</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"></td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001965-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu35.jpg" alt="Image ml322fu35.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/135649607" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">135649607</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">56946976</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">51</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">29</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"></td><td headers="hd_h_ml322.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002011-1</td><td headers="hd_h_ml322.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu36.jpg" alt="Image ml322fu36.jpg" /></div></td><td headers="hd_h_ml322.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179724" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179724</a></td><td headers="hd_h_ml322.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849983</td><td headers="hd_h_ml322.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-Synth</td><td headers="hd_h_ml322.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">66</td><td headers="hd_h_ml322.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>50</td><td headers="hd_h_ml322.t6_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml322f5"><div id="ml322.f5" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%205.%20Probe%20development%20overview%20(post-HTS).&p=BOOKS&id=148493_ml322f5.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img data-src="/books/NBK148493/bin/ml322f5.jpg" alt="Figure 5. Probe development overview (post-HTS)." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 5</span><span class="title">Probe development overview (post-HTS)</span></h3></div></article><article data-type="table-wrap" id="figobml322t7"><div id="ml322.t7" class="table"><h3><span class="label">Table 7</span><span class="title">Secondary assay results (Strosberg lab)</span></h3><div class="caption"><p>SL274 was the most potent in cell-based HCV infectivity assays and was selected to be probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>.</p></div><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t7/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t7_lrgtbl__"><table class="no_bottom_margin"><thead><tr><th id="hd_h_ml322.t7_1_1_1_1" colspan="4" rowspan="1" style="text-align:center;vertical-align:middle;">Compound Information</th><th id="hd_h_ml322.t7_1_1_1_2" colspan="2" rowspan="1" style="text-align:center;vertical-align:middle;">Core Dimerization (Alphascreen)</th><th id="hd_h_ml322.t7_1_1_1_3" rowspan="2" colspan="1" headers="hd_h_ml322.t7_1_1_1_3" style="text-align:center;vertical-align:middle;">Cytotoxicity CC<sub>50</sub> (μM)</th><th id="hd_h_ml322.t7_1_1_1_4" colspan="3" rowspan="1" style="text-align:center;vertical-align:middle;">IC<sub>50</sub> - HCV inhibition (QPCR)</th></tr><tr><th headers="hd_h_ml322.t7_1_1_1_1" id="hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL ID</th><th headers="hd_h_ml322.t7_1_1_1_1" id="hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR number</th><th headers="hd_h_ml322.t7_1_1_1_1" id="hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th headers="hd_h_ml322.t7_1_1_1_1" id="hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th headers="hd_h_ml322.t7_1_1_1_2" id="hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">%INH (15 μM)</th><th headers="hd_h_ml322.t7_1_1_1_2" id="hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">IC<sub>50</sub> [μM]</th><th headers="hd_h_ml322.t7_1_1_1_4" id="hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T1 [μM]<sup>a</sup></th><th headers="hd_h_ml322.t7_1_1_1_4" id="hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T2 [μM]<sup>a</sup></th><th headers="hd_h_ml322.t7_1_1_1_4" id="hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">TCID<sub>50</sub> [μM]<sup>b</sup></th></tr></thead><tbody><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL274</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000001966-1</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49800087</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771539</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">51</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">8.4</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">33.79</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">NA</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1.8</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.002</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL242R</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000001539-3</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">22422908</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99309211" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99309211</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">82</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1.9</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">42.6</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">8.5</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">13.7</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.191</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL270</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-01000668805-2</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9550395</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771538" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771538</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">60</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 12</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">11.96</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">NA</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.312</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL279</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002010-1</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49849980</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179723" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179723</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">66</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">180.44</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.099</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">NA</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.032</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL284</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002015-1</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49849986</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179728" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179728</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">65</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5.7</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 36</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3.067</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">NA</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.294</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL293</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002037-1</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49852628</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223097" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223097</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">79</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2.6</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">13.83</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2.7</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">7.2</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.004</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL297</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002054-1</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49859550</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233654" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233654</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">53</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5.1</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">193.21</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">18.9</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">24.3</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1.241</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL298</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002055-1</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49859547</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233655" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233655</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">83</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1.6</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9.51</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3.2</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2.2</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.665</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL415</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002229-1</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">52914829</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050594" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050594</a></td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">85</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9.9</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">22.11</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">7.7</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">NA</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.286</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL201<sup>c</sup></td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9.3</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>320</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">8.8</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">8.1</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.20</td></tr><tr><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL209<sup>c</sup></td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_1 hd_h_ml322.t7_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t7_1_1_1_2 hd_h_ml322.t7_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1.4</td><td headers="hd_h_ml322.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>100</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2.3</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3.2</td><td headers="hd_h_ml322.t7_1_1_1_4 hd_h_ml322.t7_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.286</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>a</dt><dd><div id="ml322.tfn3"><p class="no_margin">T1/T2 QPCR assays measure the ability of compounds to reduce levels of total HCV RNA in cells. NA indicates the compound did not reach an IC50 at the highest dose tested for that timepoint.</p></div></dd></dl><dl class="bkr_refwrap"><dt>b</dt><dd><div id="ml322.tfn4"><p class="no_margin">TCID<sub>50</sub> assays measure the ability of compounds to reduce levels of infectious HCV RNA; TCID<sub>50</sub> values are lower than T1/T2 QPCR values.</p></div></dd></dl><dl class="bkr_refwrap"><dt>c</dt><dd><div id="ml322.tfn5"><p class="no_margin">SL201 and SL209 are reference, prior art compounds.</p></div></dd></dl></dl></div></div></div></article><article data-type="fig" id="figobml322f7"><div id="ml322.f7" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK148493/bin/ml322f7.jpg" alt="Figure 7. Dose response curves for Probe ML322 in the Alphascreen (A), QPCR T1/T2 (B), and TCID50 assays (C)." /></div><h3><span class="label">Figure 7</span><span class="title">Dose response curves for Probe ML322 in the Alphascreen (A), QPCR T1/T2 (B), and TCID50 assays (C)</span></h3></div></article><article data-type="fig" id="figobml322f4"><div id="ml322.f4" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%204.%20Selection%20of%20SID%2049716510%20as%20the%20starting%20point%20for%20SAR%20hit%20optimization.&p=BOOKS&id=148493_ml322f4.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img data-src="/books/NBK148493/bin/ml322f4.jpg" alt="Figure 4. Selection of SID 49716510 as the starting point for SAR hit optimization." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 4</span><span class="title">Selection of SID 49716510 as the starting point for SAR hit optimization</span></h3></div></article><article data-type="fig" id="figobml322f8"><div id="ml322.f8" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%208.%20Comparison%20of%20the%20structures%20of%20the%20original%20HTS%20lead%20and%20final%20Probe%20ML322.&p=BOOKS&id=148493_ml322f8.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img data-src="/books/NBK148493/bin/ml322f8.jpg" alt="Figure 8. Comparison of the structures of the original HTS lead and final Probe ML322." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 8</span><span class="title">Comparison of the structures of the original HTS lead and final Probe ML322</span></h3></div></article><article data-type="table-wrap" id="figobml322t8"><div id="ml322.t8" class="table"><h3><span class="label">Table 8</span><span class="title">Probe ML322 is not a strong inhibitor of cytochrome P450 enzymes</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t8/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t8_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t8_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Compound ID</th><th id="hd_h_ml322.t8_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inhibition of 1A2 (%)</th><th id="hd_h_ml322.t8_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inhibition of 2C9 (%)</th><th id="hd_h_ml322.t8_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inhibition of 2D6 (%)</th><th id="hd_h_ml322.t8_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inhibition of 3A4 (%)</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t8_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;"><a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> (10 μM)</td><td headers="hd_h_ml322.t8_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>−19</b></td><td headers="hd_h_ml322.t8_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>−9</b></td><td headers="hd_h_ml322.t8_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>−11</b></td><td headers="hd_h_ml322.t8_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>9</b></td></tr><tr><td headers="hd_h_ml322.t8_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Furafylline, 1A2 standard (40 μM)</td><td headers="hd_h_ml322.t8_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>85</b></td><td headers="hd_h_ml322.t8_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>0</b></td><td headers="hd_h_ml322.t8_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>0</b></td><td headers="hd_h_ml322.t8_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>0</b></td></tr><tr><td headers="hd_h_ml322.t8_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Sulfaphenazole- 2C9 standard (10 μM)</td><td headers="hd_h_ml322.t8_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>−2</b></td><td headers="hd_h_ml322.t8_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>93</b></td><td headers="hd_h_ml322.t8_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>4</b></td><td headers="hd_h_ml322.t8_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>37</b></td></tr><tr><td headers="hd_h_ml322.t8_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Quinidine- 2D6 standard (10 μM)</td><td headers="hd_h_ml322.t8_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>−14</b></td><td headers="hd_h_ml322.t8_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>−4</b></td><td headers="hd_h_ml322.t8_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>89</b></td><td headers="hd_h_ml322.t8_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>23</b></td></tr><tr><td headers="hd_h_ml322.t8_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Ketoconazole-3A4 standard (1 μM)</td><td headers="hd_h_ml322.t8_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>−2</b></td><td headers="hd_h_ml322.t8_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>17</b></td><td headers="hd_h_ml322.t8_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>30</b></td><td headers="hd_h_ml322.t8_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><b>96</b></td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml322t9"><div id="ml322.t9" class="table"><h3><span class="label">Table 9</span><span class="title">Hepatic Microsome Stability of Selected HCV Core Inhibitors</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t9/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t9_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t9_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t9_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t9_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL Number (Assay Provider)</th><th id="hd_h_ml322.t9_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number (SRIMSC)</th><th id="hd_h_ml322.t9_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">t<sub>1/2</sub> (min) Human</th><th id="hd_h_ml322.t9_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">t<sub>1/2</sub> (min) Mouse</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t9_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49800087</td><td headers="hd_h_ml322.t9_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771539</a></td><td headers="hd_h_ml322.t9_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL274: Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a></td><td headers="hd_h_ml322.t9_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000001966-1</td><td headers="hd_h_ml322.t9_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">40</td><td headers="hd_h_ml322.t9_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">27</td></tr><tr><td headers="hd_h_ml322.t9_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">22422908</td><td headers="hd_h_ml322.t9_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99309211" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99309211</a></td><td headers="hd_h_ml322.t9_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL242R</td><td headers="hd_h_ml322.t9_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000001539-3</td><td headers="hd_h_ml322.t9_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">28</td><td headers="hd_h_ml322.t9_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2</td></tr><tr><td headers="hd_h_ml322.t9_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9550395</td><td headers="hd_h_ml322.t9_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771538" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771538</a></td><td headers="hd_h_ml322.t9_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL270</td><td headers="hd_h_ml322.t9_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-01000668805-2</td><td headers="hd_h_ml322.t9_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">43</td><td headers="hd_h_ml322.t9_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5</td></tr><tr><td headers="hd_h_ml322.t9_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49849980</td><td headers="hd_h_ml322.t9_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179723" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179723</a></td><td headers="hd_h_ml322.t9_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL279</td><td headers="hd_h_ml322.t9_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002010-1</td><td headers="hd_h_ml322.t9_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2</td><td headers="hd_h_ml322.t9_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td></tr><tr><td headers="hd_h_ml322.t9_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49859550</td><td headers="hd_h_ml322.t9_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223097" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223097</a></td><td headers="hd_h_ml322.t9_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SL293</td><td headers="hd_h_ml322.t9_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SR-03000002037-1</td><td headers="hd_h_ml322.t9_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml322.t9_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td></tr><tr><td headers="hd_h_ml322.t9_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Sunitinib</td><td headers="hd_h_ml322.t9_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t9_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">-</td><td headers="hd_h_ml322.t9_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">(Comparator)</td><td headers="hd_h_ml322.t9_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">53</td><td headers="hd_h_ml322.t9_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">15</td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml322t13"><div id="ml322.t13" class="table"><h3><span class="label">SAR Table 1</span><span class="title">Lead scaffolds from the HTS campaign</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t13/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t13_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th id="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Vendor</th><th id="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2488" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2488</a><br />HTRF IC50 (uM)</th><th id="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t13_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/463085" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 463085</a><br />Alphascreen IC50 (uM)</th><th id="hd_h_ml322.t13_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t13_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485271" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485271</a><br />HCV QPCR T1 IC50 (uM)</th><th id="hd_h_ml322.t13_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T2 IC50 (uM)</th><th id="hd_h_ml322.t13_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485280" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 485280</a><br />Cytox CC50</th><th id="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000819396-2</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219211" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219211</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422906</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu37.jpg" alt="Image ml322fu37.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.666</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t13_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.0</td><td headers="hd_h_ml322.t13_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t13_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">6.6</td><td headers="hd_h_ml322.t13_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">12</td><td headers="hd_h_ml322.t13_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">123</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000763803-4</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219208" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219208</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5280489</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Sigma Chemical</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu38.jpg" alt="Image ml322fu38.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">34.8</td><td headers="hd_h_ml322.t13_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_10 hd_h_ml322.t13_1_1_1_11 hd_h_ml322.t13_1_1_1_12" colspan="3" rowspan="2" style="text-align:center;vertical-align:middle;">Not tested due to lack of Alphascreen activity</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">171.9</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000839920-2</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219213" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219213</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24979772</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu39.jpg" alt="Image ml322fu39.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20.49</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">29.8</td><td headers="hd_h_ml322.t13_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">140.7</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000000036-3</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219207" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219207</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1780</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Sigma Chemical</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu40.jpg" alt="Image ml322fu40.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_8 hd_h_ml322.t13_1_1_1_9" colspan="2" rowspan="1" style="text-align:center;vertical-align:middle;">Not tested due to lack of HTRF activity</td><td headers="hd_h_ml322.t13_1_1_1_10 hd_h_ml322.t13_1_1_1_11 hd_h_ml322.t13_1_1_1_12" colspan="3" rowspan="1" style="text-align:center;vertical-align:middle;">Not tested due to lack of HTRF activity</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">178.6</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000812654-2</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219209" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219209</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">694792</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemBridge</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu41.jpg" alt="Image ml322fu41.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_8 hd_h_ml322.t13_1_1_1_9" colspan="2" rowspan="5" style="text-align:center;vertical-align:middle;">Not tested due to lack of HTRF activity</td><td headers="hd_h_ml322.t13_1_1_1_10 hd_h_ml322.t13_1_1_1_11 hd_h_ml322.t13_1_1_1_12" colspan="3" rowspan="5" style="text-align:center;vertical-align:middle;">Not tested due to lack of HTRF activity</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">142.9</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000812946-2</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219210" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219210</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1557339</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Pharmeks</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu42.jpg" alt="Image ml322fu42.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46.17</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24.7</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000839796-2</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219212" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219212</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24981182</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu43.jpg" alt="Image ml322fu43.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>49.751</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">27.4</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000840236-2</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219214" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219214</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24979394</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu44.jpg" alt="Image ml322fu44.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">40.43</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">148.4</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t13_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000849831-2</td><td headers="hd_h_ml322.t13_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87219215" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87219215</a></td><td headers="hd_h_ml322.t13_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9426403</td><td headers="hd_h_ml322.t13_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml322.t13_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu45.jpg" alt="Image ml322fu45.jpg" /></div></td><td headers="hd_h_ml322.t13_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">21.17</td><td headers="hd_h_ml322.t13_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t13_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">163.3</td><td headers="hd_h_ml322.t13_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml322t14"><div id="ml322.t14" class="table"><h3><span class="label">SAR Table 2</span><span class="title">Probe and lead analogs</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t14/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t14_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th id="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Vendor</th><th id="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651576" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651576</a><br />%INH at 15 μM</th><th id="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">%INH Outcome</th><th id="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">IC<sub>50</sub> Core Dimerization (μM)</th><th id="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">IC50 Outcome</th><th id="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Assay OUTCOME</th><th id="hd_h_ml322.t14_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651574" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651574</a><br />T1 IC50 (μM)</th><th id="hd_h_ml322.t14_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t14_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T2 IC50 (μM)</th><th id="hd_h_ml322.t14_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Overall Outcome</th><th id="hd_h_ml322.t14_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624575" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 624575</a><br />TCID50 (uM)</th><th id="hd_h_ml322.t14_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624583" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 624583</a><br />Cytox</th><th id="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001966-1</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771539</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800087</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu46.jpg" alt="Image ml322fu46.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">51</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">8.4</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active: PROBE <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a></td><td headers="hd_h_ml322.t14_1_1_1_11 hd_h_ml322.t14_1_1_1_12" colspan="2" rowspan="1" style="text-align:center;vertical-align:middle;">IC50 not determined at this timepoint</td><td headers="hd_h_ml322.t14_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.8</td><td headers="hd_h_ml322.t14_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active (PROBE <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>)</td><td headers="hd_h_ml322.t14_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.002</td><td headers="hd_h_ml322.t14_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active (PROBE <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>)</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">33.79</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive (Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>)</td></tr><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001539-3</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99309211" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99309211</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422908</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu47.jpg" alt="Image ml322fu47.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">82</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.9</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">8.5</td><td headers="hd_h_ml322.t14_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.7</td><td headers="hd_h_ml322.t14_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.191</td><td headers="hd_h_ml322.t14_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">42.6</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000668805-2</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771538" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771538</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9550395</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu48.jpg" alt="Image ml322fu48.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">60</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.0</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11.96</td><td headers="hd_h_ml322.t14_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_13 hd_h_ml322.t14_1_1_1_14" colspan="2" rowspan="2" style="text-align:center;vertical-align:middle;">Not tested due to probe’s lower TCID50 value</td><td headers="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.312</td><td headers="hd_h_ml322.t14_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">> 12</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002010-1</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179723" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179723</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849980</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu49.jpg" alt="Image ml322fu49.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">66</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.0</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.099</td><td headers="hd_h_ml322.t14_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.032</td><td headers="hd_h_ml322.t14_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">180.4</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002013-1</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179726" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179726</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849987</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu50.jpg" alt="Image ml322fu50.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">87</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.3</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_11 hd_h_ml322.t14_1_1_1_12 hd_h_ml322.t14_1_1_1_13 hd_h_ml322.t14_1_1_1_14 hd_h_ml322.t14_1_1_1_15 hd_h_ml322.t14_1_1_1_16 hd_h_ml322.t14_1_1_1_17" colspan="7" rowspan="1" style="text-align:center;vertical-align:middle;">Not tested due to probe’s lower cytotoxicity</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">> 4</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002015-1</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179728" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179728</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849986</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu51.jpg" alt="Image ml322fu51.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">65</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.7</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.067</td><td headers="hd_h_ml322.t14_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_13 hd_h_ml322.t14_1_1_1_14" colspan="2" rowspan="1" style="text-align:center;vertical-align:middle;">Not tested due to probe’s lower TCID50 value</td><td headers="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.294</td><td headers="hd_h_ml322.t14_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">> 36</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002037-1</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223097" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223097</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852628</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu52.jpg" alt="Image ml322fu52.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">79</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.6</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.7</td><td headers="hd_h_ml322.t14_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">7.2</td><td headers="hd_h_ml322.t14_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.004</td><td headers="hd_h_ml322.t14_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.83</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t14_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002053-1</td><td headers="hd_h_ml322.t14_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233653" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233653</a></td><td headers="hd_h_ml322.t14_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859551</td><td headers="hd_h_ml322.t14_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t14_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu53.jpg" alt="Image ml322fu53.jpg" /></div></td><td headers="hd_h_ml322.t14_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">62.4</td><td headers="hd_h_ml322.t14_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10.2</td><td headers="hd_h_ml322.t14_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.3</td><td headers="hd_h_ml322.t14_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">12.5</td><td headers="hd_h_ml322.t14_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t14_1_1_1_16 hd_h_ml322.t14_1_1_1_17" colspan="2" rowspan="1" style="text-align:center;vertical-align:middle;">Not tested due to probe’s lower TCID50 value</td><td headers="hd_h_ml322.t14_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">37.64</td><td headers="hd_h_ml322.t14_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml322t15"><div id="ml322.t15" class="table"><h3><span class="label">SAR Table 3</span><span class="title">Analogs</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t15/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t15_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th id="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Vendor</th><th id="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651576" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651576</a><br />%INH of Core dimerization at 15 μM</th><th id="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t15_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">IC<sub>50</sub> Core<br />Dimerization (μM)</th><th id="hd_h_ml322.t15_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome (Active, Inactive, or Not Tested)</th><th id="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">OVERALL OUTCOME</th><th id="hd_h_ml322.t15_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651574" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651574</a><br />T1 IC50 (μM)</th><th id="hd_h_ml322.t15_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t15_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T2 IC50 (μM)</th><th id="hd_h_ml322.t15_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t15_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Overall Outcome</th><th id="hd_h_ml322.t15_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624575" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 624575</a><br />TCID50 (uM)</th><th id="hd_h_ml322.t15_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t15_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/624583" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 624583</a><br />Cytox</th><th id="hd_h_ml322.t15_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002229-1</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050594" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050594</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914829</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu54.jpg" alt="Image ml322fu54.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">85%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.9</td><td headers="hd_h_ml322.t15_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">7.7</td><td headers="hd_h_ml322.t15_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_13 hd_h_ml322.t15_1_1_1_14" colspan="2" rowspan="1" style="text-align:center;vertical-align:middle;">T2 result not determined</td><td headers="hd_h_ml322.t15_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.286</td><td headers="hd_h_ml322.t15_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22.11</td><td headers="hd_h_ml322.t15_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002054-1</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233654" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233654</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859550</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu55.jpg" alt="Image ml322fu55.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.1</td><td headers="hd_h_ml322.t15_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">18.9</td><td headers="hd_h_ml322.t15_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24.3</td><td headers="hd_h_ml322.t15_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.241</td><td headers="hd_h_ml322.t15_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">193.2</td><td headers="hd_h_ml322.t15_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002055-1</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233655" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233655</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859547</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu56.jpg" alt="Image ml322fu56.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">83%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.6</td><td headers="hd_h_ml322.t15_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.2</td><td headers="hd_h_ml322.t15_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.2</td><td headers="hd_h_ml322.t15_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_16" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.665</td><td headers="hd_h_ml322.t15_1_1_1_17" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_18" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.51</td><td headers="hd_h_ml322.t15_1_1_1_19" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001539-1</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022352" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022352</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422908</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu57.jpg" alt="Image ml322fu57.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">97%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.9</td><td headers="hd_h_ml322.t15_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">8.5</td><td headers="hd_h_ml322.t15_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.7</td><td headers="hd_h_ml322.t15_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_16 hd_h_ml322.t15_1_1_1_17 hd_h_ml322.t15_1_1_1_18 hd_h_ml322.t15_1_1_1_19" colspan="4" rowspan="5" style="text-align:center;vertical-align:middle;">Not tested due to probe’s lower TCID50 value</td></tr><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000819396-3</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022357" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022357</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422906</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu58.jpg" alt="Image ml322fu58.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">16%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_1_1_8 hd_h_ml322.t15_1_1_1_9" colspan="2" rowspan="4" style="text-align:center;vertical-align:middle;">Low %INH value so IC50 not pursued</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_1_1_11" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">6.7</td><td headers="hd_h_ml322.t15_1_1_1_12" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_13" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">12</td><td headers="hd_h_ml322.t15_1_1_1_14" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_15" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001523-1</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022334" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022334</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422894</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu59.jpg" alt="Image ml322fu59.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">59%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_1_1_11 hd_h_ml322.t15_1_1_1_12 hd_h_ml322.t15_1_1_1_13 hd_h_ml322.t15_1_1_1_14 hd_h_ml322.t15_1_1_1_15" colspan="5" rowspan="3" style="text-align:center;vertical-align:middle;">Not tested due to probe’s lower TCID50 value</td></tr><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000550123-2</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022348" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022348</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4060916</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu60.jpg" alt="Image ml322fu60.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">40%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002051-1</td><td headers="hd_h_ml322.t15_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233651" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233651</a></td><td headers="hd_h_ml322.t15_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859548</td><td headers="hd_h_ml322.t15_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu61.jpg" alt="Image ml322fu61.jpg" /></div></td><td headers="hd_h_ml322.t15_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_1_1_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr></tbody></table><table><thead><tr><th id="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th id="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Vendor</th><th id="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/651576" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 651576</a><br />%INH of Core dimerization at 15 μM</th><th id="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th><th id="hd_h_ml322.t15_1_2_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">IC<sub>50</sub> Core<br />Dimerization (μM)</th><th id="hd_h_ml322.t15_1_2_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome (Active, Inactive, or Not Tested)</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002052-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233652" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233652</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859549</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu62.jpg" alt="Image ml322fu62.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_2_1_8 hd_h_ml322.t15_1_2_1_9" colspan="2" rowspan="32" style="text-align:center;vertical-align:middle;">Not tested because Probe<br /><a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> found to be more potent.</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001537-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022349" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022349</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">15997098</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu63.jpg" alt="Image ml322fu63.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">71%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000550140-2</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022350" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022350</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4144944</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu64.jpg" alt="Image ml322fu64.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">30%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001538-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022351" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022351</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">15998711</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu65.jpg" alt="Image ml322fu65.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">38%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000550124-2</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022354" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022354</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4060917</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu66.jpg" alt="Image ml322fu66.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000550112-2</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022355" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022355</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4060781</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu67.jpg" alt="Image ml322fu67.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001540-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022356" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022356</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422895</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu68.jpg" alt="Image ml322fu68.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">65%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001541-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022358" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022358</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">6621239</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu69.jpg" alt="Image ml322fu69.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">33%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001542-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96022359" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96022359</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">15987932</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemNavigator</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu70.jpg" alt="Image ml322fu70.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">43%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001581-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/96099796" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">96099796</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46238463</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu71.jpg" alt="Image ml322fu71.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001587-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/97302163" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">97302163</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46245561</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu72.jpg" alt="Image ml322fu72.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">87%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001588-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99205837" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99205837</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46829287</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu73.jpg" alt="Image ml322fu73.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">88%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001650-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233657" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233657</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846222</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu74.jpg" alt="Image ml322fu74.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">96%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001651-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233658" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233658</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846224</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu75.jpg" alt="Image ml322fu75.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">88%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001652-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233659" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233659</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846226</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu76.jpg" alt="Image ml322fu76.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">78%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001653-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233660" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233660</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846218</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu77.jpg" alt="Image ml322fu77.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">80%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001654-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233661" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233661</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846216</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu78.jpg" alt="Image ml322fu78.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">67%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001655-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233662" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233662</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846225</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu79.jpg" alt="Image ml322fu79.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">85%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001656-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233663" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233663</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846228</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu80.jpg" alt="Image ml322fu80.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">89%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001657-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233664" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233664</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846219</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu81.jpg" alt="Image ml322fu81.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">90%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001658-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233665" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233665</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846231</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu82.jpg" alt="Image ml322fu82.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">92%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001659-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233666" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233666</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846217</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu83.jpg" alt="Image ml322fu83.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">98%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001660-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233667" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233667</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846232</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu84.jpg" alt="Image ml322fu84.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">89%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001661-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233668" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233668</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846221</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu85.jpg" alt="Image ml322fu85.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">80%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001668-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99233670" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99233670</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46846220</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu86.jpg" alt="Image ml322fu86.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">93%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001692-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99239457" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99239457</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46850834</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu87.jpg" alt="Image ml322fu87.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">67%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001693-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99239458" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99239458</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46850820</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu88.jpg" alt="Image ml322fu88.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">80%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001963-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771536" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771536</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800086</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu89.jpg" alt="Image ml322fu89.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001964-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771537" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771537</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800089</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu90.jpg" alt="Image ml322fu90.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001982-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103947204" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103947204</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49837841</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu91.jpg" alt="Image ml322fu91.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001983-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103947205" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103947205</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49837838</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu92.jpg" alt="Image ml322fu92.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002009-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179722" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179722</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849981</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu93.jpg" alt="Image ml322fu93.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002011-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179724" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179724</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849983</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu94.jpg" alt="Image ml322fu94.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">66%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_2_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52</td><td headers="hd_h_ml322.t15_1_2_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002012-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179725" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179725</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849979</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu95.jpg" alt="Image ml322fu95.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td><td headers="hd_h_ml322.t15_1_2_1_8 hd_h_ml322.t15_1_2_1_9" colspan="2" rowspan="34" style="text-align:center;vertical-align:middle;">Not tested because Probe<br /><a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a> found to be more potent.</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002014-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179727" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179727</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849975</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu96.jpg" alt="Image ml322fu96.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002029-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223089" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223089</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852629</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu97.jpg" alt="Image ml322fu97.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002029-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223089" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223089</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852629</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu97.jpg" alt="Image ml322fu97.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002030-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223090" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223090</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852642</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu98.jpg" alt="Image ml322fu98.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002030-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223090" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223090</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852642</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu98.jpg" alt="Image ml322fu98.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002031-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223091" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223091</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852630</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu99.jpg" alt="Image ml322fu99.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002031-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223091" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223091</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852630</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu99.jpg" alt="Image ml322fu99.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002032-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223092" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223092</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852633</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu100.jpg" alt="Image ml322fu100.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002033-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223093" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223093</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852646</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu101.jpg" alt="Image ml322fu101.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002034-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223094" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223094</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852637</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu102.jpg" alt="Image ml322fu102.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">< 50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002035-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223095" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223095</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852649</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu103.jpg" alt="Image ml322fu103.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002036-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223096" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223096</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852644</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu104.jpg" alt="Image ml322fu104.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002128-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/114279612" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">114279612</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">50930769</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu105.jpg" alt="Image ml322fu105.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002169-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/116933614" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">116933614</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">50985833</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu106.jpg" alt="Image ml322fu106.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><50%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002186-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/117695952" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">117695952</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">51035445</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu107.jpg" alt="Image ml322fu107.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">−11%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002228-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050593" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050593</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914830</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu108.jpg" alt="Image ml322fu108.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">44%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002230-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050595" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050595</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914838</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu109.jpg" alt="Image ml322fu109.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002231-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050596" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050596</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914841</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu110.jpg" alt="Image ml322fu110.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">87%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002232-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050597" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050597</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914840</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu111.jpg" alt="Image ml322fu111.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">41%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000120796-2</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050598" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050598</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422913</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu112.jpg" alt="Image ml322fu112.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002233-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050599" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050599</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914831</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu113.jpg" alt="Image ml322fu113.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">37%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002234-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050600" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050600</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914835</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu114.jpg" alt="Image ml322fu114.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">63%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002252-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123054981" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123054981</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52918352</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu115.jpg" alt="Image ml322fu115.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">79%,</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002253-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123054982" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123054982</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52918345</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu116.jpg" alt="Image ml322fu116.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002256-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123054984" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123054984</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52918341</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu117.jpg" alt="Image ml322fu117.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">51%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002294-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124338630" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124338630</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53230259</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu118.jpg" alt="Image ml322fu118.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">42%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002295-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124338631" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124338631</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53230283</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu119.jpg" alt="Image ml322fu119.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">25%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002340-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124349346" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124349346</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53239851</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu120.jpg" alt="Image ml322fu120.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">12%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002341-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124349347" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124349347</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53239825</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu121.jpg" alt="Image ml322fu121.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002342-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124349348" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124349348</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53239847</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu122.jpg" alt="Image ml322fu122.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">44%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002343-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124349349" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124349349</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53239839</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu123.jpg" alt="Image ml322fu123.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">43%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002344-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124349350" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124349350</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53239842</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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|
<div class="graphic"><img src="/books/NBK148493/bin/ml322fu124.jpg" alt="Image ml322fu124.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">14%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002345-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/124349351" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">124349351</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">53239843</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu125.jpg" alt="Image ml322fu125.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">41%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr><tr><td headers="hd_h_ml322.t15_1_2_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001965-1</td><td headers="hd_h_ml322.t15_1_2_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/135649607" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">135649607</a></td><td headers="hd_h_ml322.t15_1_2_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">56946976</td><td headers="hd_h_ml322.t15_1_2_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC-synth</td><td headers="hd_h_ml322.t15_1_2_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu126.jpg" alt="Image ml322fu126.jpg" /></div></td><td headers="hd_h_ml322.t15_1_2_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">51%</td><td headers="hd_h_ml322.t15_1_2_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td><td headers="hd_h_ml322.t15_1_2_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">29</td><td headers="hd_h_ml322.t15_1_2_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Inactive</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml322f9"><div id="ml322.f9" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%209.%20HCV%20Infectivity%20assay%20method.&p=BOOKS&id=148493_ml322f9.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img data-src="/books/NBK148493/bin/ml322f9.jpg" alt="Figure 9. HCV Infectivity assay method." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 9</span><span class="title">HCV Infectivity assay method</span></h3></div></article><article data-type="table-wrap" id="figobml322t10"><div id="ml322.t10" class="table"><h3><span class="label">Table 10</span><span class="title">TCID<sub>50</sub> Results (see AID 624575)</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t10/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t10_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t10_1_1_1_1" colspan="5" rowspan="1" style="text-align:center;vertical-align:middle;">Compound Information</th><th id="hd_h_ml322.t10_1_1_1_2" colspan="2" rowspan="1" style="text-align:center;vertical-align:middle;">QPCR Assay</th></tr><tr><th headers="hd_h_ml322.t10_1_1_1_1" id="hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL</th><th headers="hd_h_ml322.t10_1_1_1_1" id="hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR Number</th><th headers="hd_h_ml322.t10_1_1_1_1" id="hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th headers="hd_h_ml322.t10_1_1_1_1" id="hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th headers="hd_h_ml322.t10_1_1_1_1" id="hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th headers="hd_h_ml322.t10_1_1_1_2" id="hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">TCID50 (uM)</th><th headers="hd_h_ml322.t10_1_1_1_2" id="hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Outcome</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL274</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001966-1</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu127.jpg" alt="Image ml322fu127.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771539" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771539</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800087</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>0.002</b></td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><b>Active (PROBE <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a>)</b></td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL242R</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000001539-3</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu128.jpg" alt="Image ml322fu128.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99309211" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99309211</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22422908</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.191</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL270</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-01000668805-2</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu129.jpg" alt="Image ml322fu129.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/103771538" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">103771538</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9550395</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.312</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL279</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002010-1</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu130.jpg" alt="Image ml322fu130.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179723" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179723</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849980</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.032</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL284</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002015-1</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu131.jpg" alt="Image ml322fu131.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104179728" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104179728</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49849986</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.294</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL293</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002037-1</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu132.jpg" alt="Image ml322fu132.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104223097" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104223097</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49852628</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.004</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL297</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002054-1</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu133.jpg" alt="Image ml322fu133.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233654" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233654</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859550</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.241</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL298</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002055-1</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu134.jpg" alt="Image ml322fu134.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/104233655" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">104233655</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49859547</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.665</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr><tr><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL415</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SR-03000002229-1</td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu135.jpg" alt="Image ml322fu135.jpg" /></div></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/123050594" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">123050594</a></td><td headers="hd_h_ml322.t10_1_1_1_1 hd_h_ml322.t10_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">52914829</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.286</td><td headers="hd_h_ml322.t10_1_1_1_2 hd_h_ml322.t10_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Active</td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml322t11"><div id="ml322.t11" class="table"><h3><span class="label">Table 11</span><span class="title">Ricerca profiling of probe ML322: SID 103771539 /CID49800087</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t11/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t11_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">Ricerca Cat</th><th id="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">Target</th><th id="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">Species</th><th id="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">% INH (10 uM)</th><th id="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">Outcome</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">203200</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adrenergic α1B</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">63</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Active</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">220320</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Transporter, Dopamine (DAT)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">62</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Active</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">203400</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adrenergic α1D</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">60</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Active</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">252810</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Muscarinic M3</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">54</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Active</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">252710</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Muscarinic M2</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">49</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">252610</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Muscarinic M1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">42</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">241000</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Imidazoline I2, Central</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">31</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">203100</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adrenergic α1A</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">27</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">226500</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GABAA, Muscimol, Central</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">26</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">239610</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Histamine H1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">24</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">255520</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Tachykinin NK1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">24</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">271700</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Serotonin (5-Hydroxytryptamine) 5-HT2B</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">21</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">226600</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GABAA, Flunitrazepam, Central</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">19</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">260210</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Opiate κ(OP2, KOP)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">18</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">232030</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Glucocorticoid</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">17</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">204410</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Transporter, Norepinephrine (NET)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">17</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">203620</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adrenergic α2A</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">16</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">232700</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Glutamate, Kainate</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">16</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">274030</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Transporter, Serotonin (5-Hydroxytryptamine)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">16</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">271110</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Serotonin (5-Hydroxytryptamine) 5-HT1A</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">15</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">204110</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adrenergic β2</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">14</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">243520</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Interleukin IL-1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">mouse</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">13</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">265900</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Potassium Channel hERG</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">13</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">233000</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Glutamate, NMDA, Phencyclidine</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">12</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">278110</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Sigma σ1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">12</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">214510</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Calcium Channel L-Type, Benzothiazepine</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">11</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">260410</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Opiate μ(OP3, MOP)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">11</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">232910</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Glutamate, NMDA, Glycine</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">10</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">200510</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adenosine A1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">217030</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Cannabinoid CB1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">239820</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Histamine H3</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">9</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">285010</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Androgen (Testosterone) AR</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">7</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">225510</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Epidermal Growth Factor (EGF)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">7</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">228610</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GABAB1A</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">7</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">219500</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Dopamine D1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">6</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">239710</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Histamine H2</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">6</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">268700</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Purinergic P2X</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rabbit</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">6</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">212510</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Bradykinin B1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">216000</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Calcium Channel N-Type</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">219700</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Dopamine D2S</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">285900</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Thyroid Hormone</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">214600</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Calcium Channel L-Type, Dihydropyridine</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">232810</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Glutamate, NMDA, Agonism</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">251600</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Melatonin MT1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">265010</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Platelet Activating Factor (PAF)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">268810</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Purinergic P2Y</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">3</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">200720</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adenosine A3</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">226400</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Transporter, GABA</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">258590</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Nicotinic Acetylcholine</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">258700</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Nicotinic Acetylcholine α, Bungarotoxin</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">268420</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Prostanoid EP4</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">270000</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Rolipram</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">rat</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">219800</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Dopamine D3</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">257010</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Neuropeptide Y Y1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">219900</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Dopamine D4.2</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−1</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">224110</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Endothelin ETB</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−1</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">264500</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Phorbol Ester</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">mouse</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−1</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">200610</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adenosine A2A</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−2</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">204010</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Adrenergic β1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−2</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">257110</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Neuropeptide Y Y2</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−3</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">260130</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Opiate δ1 (OP1, DOP)</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−3</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">212620</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Bradykinin B2</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−4</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">226010</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Estrogen ERα</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−4</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">271910</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Serotonin (5-Hydroxytryptamine) 5-HT3</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−4</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">224010</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Endothelin ETA</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−5</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">250460</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Leukotriene, Cysteinyl CysLT1</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hum</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−7</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr><tr><td headers="hd_h_ml322.t11_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">265600</td><td headers="hd_h_ml322.t11_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Potassium Channel [KATP]</td><td headers="hd_h_ml322.t11_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">hamster</td><td headers="hd_h_ml322.t11_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−11</td><td headers="hd_h_ml322.t11_1_1_1_5" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Inactive</td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml322t12"><div id="ml322.t12" class="table"><h3><span class="label">Table 12</span><span class="title">Comparison of the prior art with HCV Core probe ML322</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK148493/table/ml322.t12/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml322.t12_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml322.t12_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Compound Name</th><th id="hd_h_ml322.t12_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Structure</th><th id="hd_h_ml322.t12_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">PubChem CID</th><th id="hd_h_ml322.t12_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Probe Source</th><th id="hd_h_ml322.t12_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Present in MLSMR</th><th id="hd_h_ml322.t12_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">PubMed ID (PMID)</th><th id="hd_h_ml322.t12_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">HCV Core IC50 (μM)</th><th id="hd_h_ml322.t12_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Huh7.5 CC50 (μM)</th><th id="hd_h_ml322.t12_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">QPCR EC50</th></tr></thead><tbody><tr><td headers="hd_h_ml322.t12_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Probe <a href="/pcsubstance/?term=ML322[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML322</a></td><td headers="hd_h_ml322.t12_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu136.jpg" alt="Image ml322fu136.jpg" /></div></td><td headers="hd_h_ml322.t12_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">49800087</td><td headers="hd_h_ml322.t12_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SRIMSC Synthesis</td><td headers="hd_h_ml322.t12_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">No</td><td headers="hd_h_ml322.t12_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Manuscript in preparation</td><td headers="hd_h_ml322.t12_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">8.4</td><td headers="hd_h_ml322.t12_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>30</td><td headers="hd_h_ml322.t12_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T1 = NA; T2 = 1.8 μM; TCID50 = 2 nM</td></tr><tr><td headers="hd_h_ml322.t12_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL201</td><td headers="hd_h_ml322.t12_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu137.jpg" alt="Image ml322fu137.jpg" /></div></td><td headers="hd_h_ml322.t12_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">16759727</td><td headers="hd_h_ml322.t12_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CMLD (Boston University) CMLD003452</td><td headers="hd_h_ml322.t12_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Yes MLS000829710</td><td headers="hd_h_ml322.t12_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20035614 (Ref [<a class="bibr" href="#ml322.r17" rid="ml322.r17">17</a>], Kota 2010)</td><td headers="hd_h_ml322.t12_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.3</td><td headers="hd_h_ml322.t12_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">320</td><td headers="hd_h_ml322.t12_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T1 = 20.8 μM; T2 = 36.3 μM</td></tr><tr><td headers="hd_h_ml322.t12_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL205</td><td headers="hd_h_ml322.t12_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu138.jpg" alt="Image ml322fu138.jpg" /></div></td><td headers="hd_h_ml322.t12_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">None</td><td headers="hd_h_ml322.t12_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CMLD Synthesis</td><td headers="hd_h_ml322.t12_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">No</td><td headers="hd_h_ml322.t12_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">19896376 (Ref [<a class="bibr" href="#ml322.r12" rid="ml322.r12">12</a>], Wei 2009; (Compound 16)</td><td headers="hd_h_ml322.t12_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.0</td><td headers="hd_h_ml322.t12_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>320</td><td headers="hd_h_ml322.t12_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T1 = 4.9 μM; T2 = 0.7 μM</td></tr><tr><td headers="hd_h_ml322.t12_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL209</td><td headers="hd_h_ml322.t12_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu139.jpg" alt="Image ml322fu139.jpg" /></div></td><td headers="hd_h_ml322.t12_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">None</td><td headers="hd_h_ml322.t12_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CMLD Synthesis</td><td headers="hd_h_ml322.t12_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">No</td><td headers="hd_h_ml322.t12_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">19896376 (Ref [<a class="bibr" href="#ml322.r12" rid="ml322.r12">12</a>], Wei 2009; (Compound 17)</td><td headers="hd_h_ml322.t12_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.4</td><td headers="hd_h_ml322.t12_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">127</td><td headers="hd_h_ml322.t12_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T1 = 2.3 μM; T2 = 3.2 μM</td></tr><tr><td headers="hd_h_ml322.t12_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SL210</td><td headers="hd_h_ml322.t12_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
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<div class="graphic"><img src="/books/NBK148493/bin/ml322fu140.jpg" alt="Image ml322fu140.jpg" /></div></td><td headers="hd_h_ml322.t12_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">None</td><td headers="hd_h_ml322.t12_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CMLD Synthesis</td><td headers="hd_h_ml322.t12_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">No</td><td headers="hd_h_ml322.t12_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">19896376 (Ref [<a class="bibr" href="#ml322.r12" rid="ml322.r12">12</a>], Wei 2009; (Compound 18)</td><td headers="hd_h_ml322.t12_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.0</td><td headers="hd_h_ml322.t12_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.3</td><td headers="hd_h_ml322.t12_1_1_1_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">T1 = 3.8 μM; T2 = 3.0 μM</td></tr></tbody></table></div></div></article></div><div id="jr-scripts"><script src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/libs.min.js"> </script><script src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/jr.min.js"> </script></div></div>
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