198 lines
293 KiB
Text
198 lines
293 KiB
Text
<!DOCTYPE html>
|
|
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" class="no-js no-jr">
|
|
<head>
|
|
<!-- For pinger, set start time and add meta elements. -->
|
|
<script type="text/javascript">var ncbi_startTime = new Date();</script>
|
|
|
|
<!-- Logger begin -->
|
|
<meta name="ncbi_db" content="books">
|
|
<meta name="ncbi_pdid" content="book-part">
|
|
<meta name="ncbi_acc" content="NBK66153">
|
|
<meta name="ncbi_domain" content="mlprobe">
|
|
<meta name="ncbi_report" content="reader">
|
|
<meta name="ncbi_type" content="fulltext">
|
|
<meta name="ncbi_objectid" content="">
|
|
<meta name="ncbi_pcid" content="/NBK66153/?report=reader">
|
|
<meta name="ncbi_pagename" content="Screening for Selective Ligands for GPR55 - Antagonists - Probe Reports from the NIH Molecular Libraries Program - NCBI Bookshelf">
|
|
<meta name="ncbi_bookparttype" content="chapter">
|
|
<meta name="ncbi_app" content="bookshelf">
|
|
<!-- Logger end -->
|
|
|
|
<!--component id="Page" label="meta"/-->
|
|
<script type="text/javascript" src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/jr.boots.min.js"> </script><title>Screening for Selective Ligands for GPR55 - Antagonists - Probe Reports from the NIH Molecular Libraries Program - NCBI Bookshelf</title>
|
|
<meta charset="utf-8">
|
|
<meta name="apple-mobile-web-app-capable" content="no">
|
|
<meta name="viewport" content="initial-scale=1,minimum-scale=1,maximum-scale=1,user-scalable=no">
|
|
<meta name="jr-col-layout" content="auto">
|
|
<meta name="jr-prev-unit" content="/books/n/mlprobe/ml194/?report=reader">
|
|
<meta name="jr-next-unit" content="/books/n/mlprobe/ml190/?report=reader">
|
|
<meta name="bk-toc-url" content="/books/n/mlprobe/?report=toc">
|
|
<meta name="robots" content="INDEX,FOLLOW,NOARCHIVE">
|
|
<meta name="citation_inbook_title" content="Probe Reports from the NIH Molecular Libraries Program [Internet]">
|
|
<meta name="citation_title" content="Screening for Selective Ligands for GPR55 - Antagonists">
|
|
<meta name="citation_publisher" content="National Center for Biotechnology Information (US)">
|
|
<meta name="citation_date" content="2011/05/26">
|
|
<meta name="citation_author" content="Susanne Heynen-Genel">
|
|
<meta name="citation_author" content="Russell Dahl">
|
|
<meta name="citation_author" content="Shenghua Shi">
|
|
<meta name="citation_author" content="Loribelle Milan">
|
|
<meta name="citation_author" content="Santosh Hariharan">
|
|
<meta name="citation_author" content="Eduard Sergienko">
|
|
<meta name="citation_author" content="Michael Hedrick">
|
|
<meta name="citation_author" content="Shakeela Dad">
|
|
<meta name="citation_author" content="Derek Stonich">
|
|
<meta name="citation_author" content="Ying Su">
|
|
<meta name="citation_author" content="Michael Vicchiarelli">
|
|
<meta name="citation_author" content="Arianna Mangravita-Novo">
|
|
<meta name="citation_author" content="Layton H Smith">
|
|
<meta name="citation_author" content="Thomas DY Chung">
|
|
<meta name="citation_author" content="Haleli Sharir">
|
|
<meta name="citation_author" content="Marc G Caron">
|
|
<meta name="citation_author" content="Lawrence S Barak">
|
|
<meta name="citation_author" content="Mary E Abood">
|
|
<meta name="citation_pmid" content="22091481">
|
|
<meta name="citation_fulltext_html_url" content="https://www.ncbi.nlm.nih.gov/books/NBK66153/">
|
|
<link rel="schema.DC" href="http://purl.org/DC/elements/1.0/">
|
|
<meta name="DC.Title" content="Screening for Selective Ligands for GPR55 - Antagonists">
|
|
<meta name="DC.Type" content="Text">
|
|
<meta name="DC.Publisher" content="National Center for Biotechnology Information (US)">
|
|
<meta name="DC.Contributor" content="Susanne Heynen-Genel">
|
|
<meta name="DC.Contributor" content="Russell Dahl">
|
|
<meta name="DC.Contributor" content="Shenghua Shi">
|
|
<meta name="DC.Contributor" content="Loribelle Milan">
|
|
<meta name="DC.Contributor" content="Santosh Hariharan">
|
|
<meta name="DC.Contributor" content="Eduard Sergienko">
|
|
<meta name="DC.Contributor" content="Michael Hedrick">
|
|
<meta name="DC.Contributor" content="Shakeela Dad">
|
|
<meta name="DC.Contributor" content="Derek Stonich">
|
|
<meta name="DC.Contributor" content="Ying Su">
|
|
<meta name="DC.Contributor" content="Michael Vicchiarelli">
|
|
<meta name="DC.Contributor" content="Arianna Mangravita-Novo">
|
|
<meta name="DC.Contributor" content="Layton H Smith">
|
|
<meta name="DC.Contributor" content="Thomas DY Chung">
|
|
<meta name="DC.Contributor" content="Haleli Sharir">
|
|
<meta name="DC.Contributor" content="Marc G Caron">
|
|
<meta name="DC.Contributor" content="Lawrence S Barak">
|
|
<meta name="DC.Contributor" content="Mary E Abood">
|
|
<meta name="DC.Date" content="2011/05/26">
|
|
<meta name="DC.Identifier" content="https://www.ncbi.nlm.nih.gov/books/NBK66153/">
|
|
<meta name="description" content="The orphan G protein coupled receptor, GPR55, has gained notoriety because of its putative identification as a cannabinoid receptor subtype. The significance of this assignment should not be underestimated given the importance of cannabinoids to drug abuse and the potential utility of cannabinoid ligands in treating behavioral disorders leading to conditions such as obesity. The validity of assigning GPR55 to the cannabinoid family is problematic based upon the discovery that endogenous compounds not related to cannabinoid receptor ligands also bind and signal through GPR55. As a consequence, it is important to identify GPR55-selective ligands that can precisely define GPR55’s role in regulating addictive behaviors. Cannabidiol has been reported as a GPR55 antagonist in the literature to GPR55 activation by O1062, but this could not be confirmed our laboratory (Dr. Abood & Dr. Barak). There are no other known small molecule antagonists of GPR55 reported to date, only the CB1 inverse agonist/antagonists SR141716A (3.9 μM) and AM251 (9.6 μM). We have identified 3 potent and selective antagonists for GPR55, that represent 3 different chemical core scaffolds: 1) a quinoline aryl sulfonamide, ML193 (CID1261822) with a 221 nM potency for GPR55 and >145-fold, >27-fold and >145-fold antagonist selectivity against GPR35, CB1 and CB2, respectively and >145-fold agonist selectivity against all of these counter-receptors; 2) a thienopyrimidine, ML192 (CID1434953) with 1080 nM potency for GPR55 and >45-fold antagonist and agonist selectivity against GPR35, CB1 and CB2; and 3) a piperadinyloxadiazolone, ML191 (23612552) with 160 nM potency for GPR55 and >100-fold selectivity against GPR35, CB1 and CB2. All three probes also are active in inhibiting the downstream responses of ERK phosphorylation and PKC β II translocation.">
|
|
<meta name="og:title" content="Screening for Selective Ligands for GPR55 - Antagonists">
|
|
<meta name="og:type" content="book">
|
|
<meta name="og:description" content="The orphan G protein coupled receptor, GPR55, has gained notoriety because of its putative identification as a cannabinoid receptor subtype. The significance of this assignment should not be underestimated given the importance of cannabinoids to drug abuse and the potential utility of cannabinoid ligands in treating behavioral disorders leading to conditions such as obesity. The validity of assigning GPR55 to the cannabinoid family is problematic based upon the discovery that endogenous compounds not related to cannabinoid receptor ligands also bind and signal through GPR55. As a consequence, it is important to identify GPR55-selective ligands that can precisely define GPR55’s role in regulating addictive behaviors. Cannabidiol has been reported as a GPR55 antagonist in the literature to GPR55 activation by O1062, but this could not be confirmed our laboratory (Dr. Abood & Dr. Barak). There are no other known small molecule antagonists of GPR55 reported to date, only the CB1 inverse agonist/antagonists SR141716A (3.9 μM) and AM251 (9.6 μM). We have identified 3 potent and selective antagonists for GPR55, that represent 3 different chemical core scaffolds: 1) a quinoline aryl sulfonamide, ML193 (CID1261822) with a 221 nM potency for GPR55 and >145-fold, >27-fold and >145-fold antagonist selectivity against GPR35, CB1 and CB2, respectively and >145-fold agonist selectivity against all of these counter-receptors; 2) a thienopyrimidine, ML192 (CID1434953) with 1080 nM potency for GPR55 and >45-fold antagonist and agonist selectivity against GPR35, CB1 and CB2; and 3) a piperadinyloxadiazolone, ML191 (23612552) with 160 nM potency for GPR55 and >100-fold selectivity against GPR35, CB1 and CB2. All three probes also are active in inhibiting the downstream responses of ERK phosphorylation and PKC β II translocation.">
|
|
<meta name="og:url" content="https://www.ncbi.nlm.nih.gov/books/NBK66153/">
|
|
<meta name="og:site_name" content="NCBI Bookshelf">
|
|
<meta name="og:image" content="https://www.ncbi.nlm.nih.gov/corehtml/pmc/pmcgifs/bookshelf/thumbs/th-mlprobe-lrg.png">
|
|
<meta name="twitter:card" content="summary">
|
|
<meta name="twitter:site" content="@ncbibooks">
|
|
<meta name="bk-non-canon-loc" content="/books/n/mlprobe/ml193/?report=reader">
|
|
<link rel="canonical" href="https://www.ncbi.nlm.nih.gov/books/NBK66153/">
|
|
<link href="https://fonts.googleapis.com/css?family=Archivo+Narrow:400,700,400italic,700italic&subset=latin" rel="stylesheet" type="text/css">
|
|
<link rel="stylesheet" href="/corehtml/pmc/jatsreader/ptpmc_3.22/css/libs.min.css">
|
|
<link rel="stylesheet" href="/corehtml/pmc/jatsreader/ptpmc_3.22/css/jr.min.css">
|
|
<meta name="format-detection" content="telephone=no">
|
|
<link rel="stylesheet" href="/corehtml/pmc/css/bookshelf/2.26/css/books.min.css" type="text/css">
|
|
<link rel="stylesheet" href="/corehtml/pmc/css/bookshelf/2.26/css//books_print.min.css" type="text/css" media="print">
|
|
<link rel="stylesheet" href="/corehtml/pmc/css/bookshelf/2.26/css/books_reader.min.css" type="text/css">
|
|
<style type="text/css">p a.figpopup{display:inline !important} .bk_tt {font-family: monospace} .first-line-outdent .bk_ref {display: inline} .body-content h2, .body-content .h2 {border-bottom: 1px solid #97B0C8} .body-content h2.inline {border-bottom: none} a.page-toc-label , .jig-ncbismoothscroll a {text-decoration:none;border:0 !important} .temp-labeled-list .graphic {display:inline-block !important} .temp-labeled-list img{width:100%}</style>
|
|
|
|
<link rel="shortcut icon" href="//www.ncbi.nlm.nih.gov/favicon.ico">
|
|
<meta name="ncbi_phid" content="CE8BFED17D65FEE10000000000BA00A5.m_5">
|
|
<meta name='referrer' content='origin-when-cross-origin'/><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4216699/css/3852956/3849091.css"></head>
|
|
<body>
|
|
<!-- Book content! -->
|
|
|
|
|
|
<div id="jr" data-jr-path="/corehtml/pmc/jatsreader/ptpmc_3.22/"><div class="jr-unsupported"><table class="modal"><tr><td><span class="attn inline-block"></span><br />Your browser does not support the NLM PubReader view.<br />Go to <a href="/pmc/about/pr-browsers/">this page</a> to see a list of supported browsers<br />or return to the <br /><a href="/books/NBK66153/?report=classic">regular view</a>.</td></tr></table></div><div id="jr-ui" class="hidden"><nav id="jr-head"><div class="flexh tb"><div id="jr-tb1"><a id="jr-links-sw" class="hidden" title="Links"><svg xmlns="http://www.w3.org/2000/svg" version="1.1" x="0px" y="0px" viewBox="0 0 70.6 85.3" style="enable-background:new 0 0 70.6 85.3;vertical-align:middle" xml:space="preserve" width="24" height="24">
|
|
<style type="text/css">.st0{fill:#939598;}</style>
|
|
<g>
|
|
<path class="st0" d="M36,0C12.8,2.2-22.4,14.6,19.6,32.5C40.7,41.4-30.6,14,35.9,9.8"></path>
|
|
<path class="st0" d="M34.5,85.3c23.2-2.2,58.4-14.6,16.4-32.5c-21.1-8.9,50.2,18.5-16.3,22.7"></path>
|
|
<path class="st0" d="M34.7,37.1c66.5-4.2-4.8-31.6,16.3-22.7c42.1,17.9,6.9,30.3-16.4,32.5h1.7c-66.2,4.4,4.8,31.6-16.3,22.7 c-42.1-17.9-6.9-30.3,16.4-32.5"></path>
|
|
</g>
|
|
</svg> Books</a></div><div class="jr-rhead f1 flexh"><div class="head"><a href="/books/n/mlprobe/ml194/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M75,30 c-80,60 -80,0 0,60 c-30,-60 -30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Prev</text></svg></a></div><div class="body"><div class="t">Screening for Selective Ligands for GPR55 - Antagonists</div><div class="j">Probe Reports from the NIH Molecular Libraries Program [Internet]</div></div><div class="tail"><a href="/books/n/mlprobe/ml190/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M25,30c80,60 80,0 0,60 c30,-60 30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Next</text></svg></a></div></div><div id="jr-tb2"><a id="jr-bkhelp-sw" class="btn wsprkl hidden" title="Help with NLM PubReader">?</a><a id="jr-help-sw" class="btn wsprkl hidden" title="Settings and typography in NLM PubReader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512" preserveAspectRatio="none"><path d="M462,283.742v-55.485l-29.981-10.662c-11.431-4.065-20.628-12.794-25.274-24.001 c-0.002-0.004-0.004-0.009-0.006-0.013c-4.659-11.235-4.333-23.918,0.889-34.903l13.653-28.724l-39.234-39.234l-28.72,13.652 c-10.979,5.219-23.68,5.546-34.908,0.889c-0.005-0.002-0.01-0.003-0.014-0.005c-11.215-4.65-19.933-13.834-24-25.273L283.741,50 h-55.484l-10.662,29.981c-4.065,11.431-12.794,20.627-24.001,25.274c-0.005,0.002-0.009,0.004-0.014,0.005 c-11.235,4.66-23.919,4.333-34.905-0.889l-28.723-13.653l-39.234,39.234l13.653,28.721c5.219,10.979,5.545,23.681,0.889,34.91 c-0.002,0.004-0.004,0.009-0.006,0.013c-4.649,11.214-13.834,19.931-25.271,23.998L50,228.257v55.485l29.98,10.661 c11.431,4.065,20.627,12.794,25.274,24c0.002,0.005,0.003,0.01,0.005,0.014c4.66,11.236,4.334,23.921-0.888,34.906l-13.654,28.723 l39.234,39.234l28.721-13.652c10.979-5.219,23.681-5.546,34.909-0.889c0.005,0.002,0.01,0.004,0.014,0.006 c11.214,4.649,19.93,13.833,23.998,25.271L228.257,462h55.484l10.595-29.79c4.103-11.538,12.908-20.824,24.216-25.525 c0.005-0.002,0.009-0.004,0.014-0.006c11.127-4.628,23.694-4.311,34.578,0.863l28.902,13.738l39.234-39.234l-13.66-28.737 c-5.214-10.969-5.539-23.659-0.886-34.877c0.002-0.005,0.004-0.009,0.006-0.014c4.654-11.225,13.848-19.949,25.297-24.021 L462,283.742z M256,331.546c-41.724,0-75.548-33.823-75.548-75.546s33.824-75.547,75.548-75.547 c41.723,0,75.546,33.824,75.546,75.547S297.723,331.546,256,331.546z"></path></svg></a><a id="jr-fip-sw" class="btn wsprkl hidden" title="Find"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 550 600" preserveAspectRatio="none"><path fill="none" stroke="#000" stroke-width="36" stroke-linecap="round" style="fill:#FFF" d="m320,350a153,153 0 1,0-2,2l170,170m-91-117 110,110-26,26-110-110"></path></svg></a><a id="jr-rtoc-sw" class="btn wsprkl hidden" title="Table of Contents"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M20,20h10v8H20V20zM36,20h44v8H36V20zM20,37.33h10v8H20V37.33zM36,37.33h44v8H36V37.33zM20,54.66h10v8H20V54.66zM36,54.66h44v8H36V54.66zM20,72h10v8 H20V72zM36,72h44v8H36V72z"></path></svg></a></div></div></nav><nav id="jr-dash" class="noselect"><nav id="jr-dash" class="noselect"><div id="jr-pi" class="hidden"><a id="jr-pi-prev" class="hidden" title="Previous page"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M75,30 c-80,60 -80,0 0,60 c-30,-60 -30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Prev</text></svg></a><div class="pginfo">Page <i class="jr-pg-pn">0</i> of <i class="jr-pg-lp">0</i></div><a id="jr-pi-next" class="hidden" title="Next page"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M25,30c80,60 80,0 0,60 c30,-60 30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Next</text></svg></a></div><div id="jr-is-tb"><a id="jr-is-sw" class="btn wsprkl hidden" title="Switch between Figures/Tables strip and Progress bar"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><rect x="10" y="40" width="20" height="20"></rect><rect x="40" y="40" width="20" height="20"></rect><rect x="70" y="40" width="20" height="20"></rect></svg></a></div><nav id="jr-istrip" class="istrip hidden"><a id="jr-is-prev" href="#" class="hidden" title="Previous"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M80,40 60,65 80,90 70,90 50,65 70,40z M50,40 30,65 50,90 40,90 20,65 40,40z"></path><text x="35" y="25" textLength="60" style="font-size:25px">Prev</text></svg></a><a id="jr-is-next" href="#" class="hidden" title="Next"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M20,40 40,65 20,90 30,90 50,65 30,40z M50,40 70,65 50,90 60,90 80,65 60,40z"></path><text x="15" y="25" textLength="60" style="font-size:25px">Next</text></svg></a></nav><nav id="jr-progress"></nav></nav></nav><aside id="jr-links-p" class="hidden flexv"><div class="tb sk-htbar flexh"><div><a class="jr-p-close btn wsprkl">Done</a></div><div class="title-text f1">NCBI Bookshelf</div></div><div class="cnt lol f1"><a href="/books/">Home</a><a href="/books/browse/">Browse All Titles</a><a class="btn share" target="_blank" rel="noopener noreferrer" href="https://www.facebook.com/sharer/sharer.php?u=https://www.ncbi.nlm.nih.gov/books/NBK66153/"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 33 33" style="vertical-align:middle" width="24" height="24" preserveAspectRatio="none"><g><path d="M 17.996,32L 12,32 L 12,16 l-4,0 l0-5.514 l 4-0.002l-0.006-3.248C 11.993,2.737, 13.213,0, 18.512,0l 4.412,0 l0,5.515 l-2.757,0 c-2.063,0-2.163,0.77-2.163,2.209l-0.008,2.76l 4.959,0 l-0.585,5.514L 18,16L 17.996,32z"></path></g></svg> Share on Facebook</a><a class="btn share" target="_blank" rel="noopener noreferrer" href="https://twitter.com/intent/tweet?url=https://www.ncbi.nlm.nih.gov/books/NBK66153/&text=Screening%20for%20Selective%20Ligands%20for%20GPR55%20-%20Antagonists"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 33 33" style="vertical-align:middle" width="24" height="24"><g><path d="M 32,6.076c-1.177,0.522-2.443,0.875-3.771,1.034c 1.355-0.813, 2.396-2.099, 2.887-3.632 c-1.269,0.752-2.674,1.299-4.169,1.593c-1.198-1.276-2.904-2.073-4.792-2.073c-3.626,0-6.565,2.939-6.565,6.565 c0,0.515, 0.058,1.016, 0.17,1.496c-5.456-0.274-10.294-2.888-13.532-6.86c-0.565,0.97-0.889,2.097-0.889,3.301 c0,2.278, 1.159,4.287, 2.921,5.465c-1.076-0.034-2.088-0.329-2.974-0.821c-0.001,0.027-0.001,0.055-0.001,0.083 c0,3.181, 2.263,5.834, 5.266,6.438c-0.551,0.15-1.131,0.23-1.73,0.23c-0.423,0-0.834-0.041-1.235-0.118 c 0.836,2.608, 3.26,4.506, 6.133,4.559c-2.247,1.761-5.078,2.81-8.154,2.81c-0.53,0-1.052-0.031-1.566-0.092 c 2.905,1.863, 6.356,2.95, 10.064,2.95c 12.076,0, 18.679-10.004, 18.679-18.68c0-0.285-0.006-0.568-0.019-0.849 C 30.007,8.548, 31.12,7.392, 32,6.076z"></path></g></svg> Share on Twitter</a></div></aside><aside id="jr-rtoc-p" class="hidden flexv"><div class="tb sk-htbar flexh"><div><a class="jr-p-close btn wsprkl">Done</a></div><div class="title-text f1">Table of Content</div></div><div class="cnt lol f1"><a href="/books/n/mlprobe/?report=reader">Title Information</a><a href="/books/n/mlprobe/toc/?report=reader">Table of Contents Page</a></div></aside><aside id="jr-help-p" class="hidden flexv"><div class="tb sk-htbar flexh"><div><a class="jr-p-close btn wsprkl">Done</a></div><div class="title-text f1">Settings</div></div><div class="cnt f1"><div id="jr-typo-p" class="typo"><div><a class="sf btn wsprkl">A-</a><a class="lf btn wsprkl">A+</a></div><div><a class="bcol-auto btn wsprkl"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 200 100" preserveAspectRatio="none"><text x="10" y="70" style="font-size:60px;font-family: Trebuchet MS, ArialMT, Arial, sans-serif" textLength="180">AUTO</text></svg></a><a class="bcol-1 btn wsprkl"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M15,25 85,25zM15,40 85,40zM15,55 85,55zM15,70 85,70z"></path></svg></a><a class="bcol-2 btn wsprkl"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M5,25 45,25z M55,25 95,25zM5,40 45,40z M55,40 95,40zM5,55 45,55z M55,55 95,55zM5,70 45,70z M55,70 95,70z"></path></svg></a></div></div><div class="lol"><a class="" href="/books/NBK66153/?report=classic">Switch to classic view</a><a href="/books/NBK66153/pdf/Bookshelf_NBK66153.pdf">PDF (1.0M)</a><a href="/books/NBK66153/?report=printable">Print View</a></div></div></aside><aside id="jr-bkhelp-p" class="hidden flexv"><div class="tb sk-htbar flexh"><div><a class="jr-p-close btn wsprkl">Done</a></div><div class="title-text f1">Help</div></div><div class="cnt f1 lol"><a id="jr-helpobj-sw" data-path="/corehtml/pmc/jatsreader/ptpmc_3.22/" data-href="/corehtml/pmc/jatsreader/ptpmc_3.22/img/bookshelf/help.xml" href="">Help</a><a href="mailto:info@ncbi.nlm.nih.gov?subject=PubReader%20feedback%20%2F%20NBK66153%20%2F%20sid%3ACE8B5AF87C7FFCB1_0191SID%20%2F%20phid%3ACE8BFED17D65FEE10000000000BA00A5.4">Send us feedback</a><a id="jr-about-sw" data-path="/corehtml/pmc/jatsreader/ptpmc_3.22/" data-href="/corehtml/pmc/jatsreader/ptpmc_3.22/img/bookshelf/about.xml" href="">About PubReader</a></div></aside><aside id="jr-objectbox" class="thidden hidden"><div class="jr-objectbox-close wsprkl">✘</div><div class="jr-objectbox-inner cnt"><div class="jr-objectbox-drawer"></div></div></aside><nav id="jr-pm-left" class="hidden"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 40 800" preserveAspectRatio="none"><text font-stretch="ultra-condensed" x="800" y="-15" text-anchor="end" transform="rotate(90)" font-size="18" letter-spacing=".1em">Previous Page</text></svg></nav><nav id="jr-pm-right" class="hidden"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 40 800" preserveAspectRatio="none"><text font-stretch="ultra-condensed" x="800" y="-15" text-anchor="end" transform="rotate(90)" font-size="18" letter-spacing=".1em">Next Page</text></svg></nav><nav id="jr-fip" class="hidden"><nav id="jr-fip-term-p"><input type="search" placeholder="search this page" id="jr-fip-term" autocorrect="off" autocomplete="off" /><a id="jr-fip-mg" class="wsprkl btn" title="Find"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 550 600" preserveAspectRatio="none"><path fill="none" stroke="#000" stroke-width="36" stroke-linecap="round" style="fill:#FFF" d="m320,350a153,153 0 1,0-2,2l170,170m-91-117 110,110-26,26-110-110"></path></svg></a><a id="jr-fip-done" class="wsprkl btn" title="Dismiss find">✘</a></nav><nav id="jr-fip-info-p"><a id="jr-fip-prev" class="wsprkl btn" title="Jump to previuos match">◀</a><button id="jr-fip-matches">no matches yet</button><a id="jr-fip-next" class="wsprkl btn" title="Jump to next match">▶</a></nav></nav></div><div id="jr-epub-interstitial" class="hidden"></div><div id="jr-content"><article data-type="main"><div class="main-content lit-style" itemscope="itemscope" itemtype="http://schema.org/CreativeWork"><div class="meta-content fm-sec"><div class="fm-sec"><h1 id="_NBK66153_"><span class="title" itemprop="name">Screening for Selective Ligands for GPR55 - Antagonists</span></h1><p class="contribs">Heynen-Genel S, Dahl R, Shi S, et al.</p><p class="fm-aai"><a href="#_NBK66153_pubdet_">Publication Details</a></p></div></div><div class="jig-ncbiinpagenav body-content whole_rhythm" data-jigconfig="allHeadingLevels: ['h2'],smoothScroll: false" itemprop="text"><div id="_abs_rndgid_" itemprop="description"><p>The orphan G protein coupled receptor, GPR55, has gained notoriety because of its putative identification as a cannabinoid receptor subtype. The significance of this assignment should not be underestimated given the importance of cannabinoids to drug abuse and the potential utility of cannabinoid ligands in treating behavioral disorders leading to conditions such as obesity. The validity of assigning GPR55 to the cannabinoid family is problematic based upon the discovery that endogenous compounds not related to cannabinoid receptor ligands also bind and signal through GPR55. As a consequence, it is important to identify GPR55-selective ligands that can precisely define GPR55’s role in regulating addictive behaviors. Cannabidiol has been reported as a GPR55 antagonist in the literature to GPR55 activation by O1062, but this could not be confirmed our laboratory (Dr. Abood & Dr. Barak). There are no other known small molecule antagonists of GPR55 reported to date, only the CB1 inverse agonist/antagonists SR141716A (3.9 μM) and AM251 (9.6 μM). We have identified 3 potent and selective antagonists for GPR55, that represent 3 different chemical core scaffolds: 1) a quinoline aryl sulfonamide, <b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=abstract&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b> (CID1261822) with a 221 nM potency for GPR55 and >145-fold, >27-fold and >145-fold antagonist selectivity against GPR35, CB1 and CB2, respectively and >145-fold agonist selectivity against all of these counter-receptors; 2) a thienopyrimidine, <b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=abstract&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b> (CID1434953) with 1080 nM potency for GPR55 and >45-fold antagonist and agonist selectivity against GPR35, CB1 and CB2; and 3) a piperadinyloxadiazolone, <b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=abstract&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b> (23612552) with 160 nM potency for GPR55 and >100-fold selectivity against GPR35, CB1 and CB2. All three probes also are active in inhibiting the downstream responses of ERK phosphorylation and PKC β II translocation.</p></div><div class="h2"></div><p><b>Probe project:</b> Selective GPR55 Antagonists: Part 2</p><p><b>Assigned Assay Grant #:</b> 1X01 DA026205-01 (<i>revised on transfer to NIDA</i>) - <i>previously 1X01 MH084187-01</i></p><p><b>Screening Center Name & PI:</b> Sanford-Burnham Center for Chemical Genomics & John C. Reed</p><p><b>Chemistry Center Name & PI:</b> same as above</p><p><b>Assay Submitter & Institution:</b> Mary E. Abood, California Pacific Medical Center Research Institute (currently at Temple University, Center for Substance Abuse Research).</p><p><b>Collaborating PI:</b> Lawrence S. Barak, Duke University</p><p><b>PubChem Summary Bioassay Identifier (AID):</b>
|
|
<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2026" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2026</a></p><div id="ml193.s1"><h2 id="_ml193_s1_">Probe Structure & Characteristics</h2><p><i>This Center Probe Report provides the first description of 3 novel chemical probes that represent three chemical series for GPR55, an orphan GPCR receptor:</i></p><ul class="simple-list"><li class="half_rhythm"><div><i><b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b></i>
|
|
<i>(quinoline aryl sulfonamide),</i></div></li><li class="half_rhythm"><div><i><b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b></i>
|
|
<i>(thienopyrimidine), and</i></div></li><li class="half_rhythm"><div><i><b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b></i>
|
|
<i>(piperadinyloxadiazolone).</i></div></li></ul><div id="ml193.fu1" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu1.jpg" alt="ML193." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></span></h3><div class="caption"><p>CID 1261822</p></div></div><div id="ml193.fu2" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu2.jpg" alt="ML192." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></span></h3><div class="caption"><p>CID 1434953</p></div></div><div id="ml193.fu3" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu3.jpg" alt="ML191." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></span></h3><div class="caption"><p>CID 23612552</p></div></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193tu1"><a href="/books/NBK66153/table/ml193.tu1/?report=objectonly" target="object" title="Table" class="img_link icnblk_img figpopup" rid-figpopup="figml193tu1" rid-ob="figobml193tu1"><img class="small-thumb" src="/books/NBK66153/table/ml193.tu1/?report=thumb" src-large="/books/NBK66153/table/ml193.tu1/?report=previmg" alt="Image " /></a><div class="icnblk_cntnt"><h4 id="ml193.tu1"><a href="/books/NBK66153/table/ml193.tu1/?report=objectonly" target="object" rid-ob="figobml193tu1">Table</a></h4></div></div></div><div id="ml193.s2"><h2 id="_ml193_s2_">Recommendations for the scientific use of this probe</h2><p>This proposal will provide tools for delineating the biochemistry of GPR55, potentially provide compounds for targeted therapeutics of pathways underlying addictive behaviors, and clarify our understanding of the molecular basis of addiction in general. In addition, recent studies suggest a role for GPR55 in inflammatory and neuropathic pain, bone development and cancer (<a class="bibr" href="#ml193.r1" rid="ml193.r1 ml193.r2 ml193.r3 ml193.r4 ml193.r5 ml193.r6 ml193.r7 ml193.r8">1–8</a>). The availability of high potency antagonists will facilitate the study of the biological roles of this receptor. Antagonists from this project provide novel tool compounds in which to evaluate GPR55 signaling in cells and in the future in animals. All other current putative tool compounds, were selected on the basis of being cannabinoid ligands, whereas new scaffolds will have unblemished pedigrees in that area.</p></div><div id="ml193.s3"><h2 id="_ml193_s3_">1. Scientific Rationale for Project</h2><div id="ml193.s4"><h3>Specific Aims (from the originating grant)</h3><p><b>The specific aims of the originating proposal were: 1) To identify small molecule agonists of GPR55 by high content screening of MLSCN (Molecular Library Screening Center Network) libraries (</b><i>covered in this probe report</i><b>) and 2) To identify small molecule antagonists of GPR55 by high content screening of MLSCN libraries</b> We propose to provide the MLSCN with cell lines expressing the receptors and β-arrestin fluorescent protein biosensors. These studies will provide tools for delineating the biochemistry of GPR55 to enable an understanding of the molecular basis of cannabinoid addiction and treatment.</p></div><div id="ml193.s5"><h3>Background and Significance</h3><p>Cannabidiol has been reported as a GPR55 antagonist in the literature (<a class="bibr" href="#ml193.r9" rid="ml193.r9">9</a>,<a class="bibr" href="#ml193.r10" rid="ml193.r10">10</a>) to GPR55 activation by O1062, but could not be confirmed as such in the assay provider’s laboratory. There are no other known small molecule antagonists of GPR55 reported to date, so this screen did not have any positive controls other than the absence of agonist. The only previously reported GPR55 agonists were lysophosphatidylinositol (LPI) and the CB1 inverse agonist/antagonists SR141716A and AM251 (see <a class="figpopup" href="/books/NBK66153/figure/ml193.f1/?report=objectonly" target="object" rid-figpopup="figml193f1" rid-ob="figobml193f1">Figure 1</a> below). These compounds had the following reported GPR55 potencies: SR141716A GPR55 EC50=3.9 μM; AM251 GPR55 EC50=9.6 μM; and LPI GPR55 EC50=1.2 μM. However, the two small molecule, drug-like ligands SR14176A and AM251 are not selective.</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml193f1" co-legend-rid="figlgndml193f1"><a href="/books/NBK66153/figure/ml193.f1/?report=objectonly" target="object" title="Figure 1" class="img_link icnblk_img figpopup" rid-figpopup="figml193f1" rid-ob="figobml193f1"><img class="small-thumb" src="/books/NBK66153/bin/ml193f1.gif" src-large="/books/NBK66153/bin/ml193f1.jpg" alt="Figure 1. Previously reported GPR55 agonists." /></a><div class="icnblk_cntnt" id="figlgndml193f1"><h4 id="ml193.f1"><a href="/books/NBK66153/figure/ml193.f1/?report=objectonly" target="object" rid-ob="figobml193f1">Figure 1</a></h4><p class="float-caption no_bottom_margin">Previously reported GPR55 agonists. </p></div></div><p>Marijuana is the most widely abused illegal drug and its spectrum of effects suggests that several receptors are responsible for its activity. Two cannabinoid receptor subtypes, CB1 and CB2, have been identified; however, in support of the notion that other cannabinoid receptors remain to be identified, the complex pharmacological properties of exogenous cannabinoids and endocannabinoids are not fully explained by CB1 and CB2 signal transduction. Recently the orphan G protein coupled receptor, GPR55, was declared as one of the missing cannabinoid receptor subtypes. However, the validity of this assignment remains in question based upon the identification of another class of GPR55 ligands. As a consequence of the identification of GPR55 as a target for cannabinoid binding, it becomes important to identify GPR55-selective ligands that can define its role in addiction. The primary high throughput assays proposed for identifying GPR55 active compounds are based upon the ability to monitor the activation states of ligand-bound GPR receptors using high content imaging employing β-arrestin green fluorescent protein biosensors. The pharmacology of GPR55 remains complex, with different laboratories reporting distinct ligand selectivity. However, it has become clear that GPR55 does share some ligands with the known cannabinoid receptors. The availability of selective high potency antagonists will facilitate the study of this receptor.</p><div id="ml193.s6"><h4>a. Describe the original goal for probe characteristics as identified in the CPDP</h4><p>These studies will provide tools for delineating the biochemistry of GPR55 to enable an understanding of the molecular basis of cannabinoid addiction and treatment as well as to delineate the pharmacology of this receptor. The probe criteria are potency of better than 1–5μM and better than 10-fold selectivity against CB1, CB2, and GPR35.</p></div><div id="ml193.s7"><h4>b. For each assay implemented and screening run please provide</h4><div id="ml193.s8"><h5>i. PubChem Bioassay Name(s), AID(s), Assay-Type (Primary, DR, Counterscreen, Secondary)</h5><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t1"><a href="/books/NBK66153/table/ml193.t1/?report=objectonly" target="object" title="Table 1" class="img_link icnblk_img figpopup" rid-figpopup="figml193t1" rid-ob="figobml193t1"><img class="small-thumb" src="/books/NBK66153/table/ml193.t1/?report=thumb" src-large="/books/NBK66153/table/ml193.t1/?report=previmg" alt="Table 1. Summary of Assays and AIDs." /></a><div class="icnblk_cntnt"><h4 id="ml193.t1"><a href="/books/NBK66153/table/ml193.t1/?report=objectonly" target="object" rid-ob="figobml193t1">Table 1</a></h4><p class="float-caption no_bottom_margin">Summary of Assays and AIDs. </p></div></div></div><div id="ml193.s9"><h5>ii. Assay Rationale & Description (when describing primary screen it would be useful to see standard metrics like, Z′, S:B for the optimized assay). Table of reagents and source</h5><div id="ml193.s10"><h5>Primary Screen</h5><p>The read-out needed to be independent of the orphan status of GPR55. Therefore the well-characterized pathway of β-arrestin mediated internalization following receptor activation was chosen. In the particular embodiment, a β-arrestin biosensor comprised of GFP fused to β-arrestin protein was stably expressed in an engineered stable cell line and GPR55E was cloned into this cell line. GPR55E, a C-tail modified variant of GPR55, with greater β-arrestin responsiveness and similar pharmacology was used for the primary assay and will be known throughout the probe report as GPR55 Kapur <i>et al</i>. (<a class="bibr" href="#ml193.r11" rid="ml193.r11">11</a>). This image-based high-content screen (HCS) is based then on the fluorescence redistribution of GFP-β-arrestin complex bound receptors from a homogeneous distribution in the cytoplasm via the plasma membrane into clathrin-coated intracellular pit, then into vesicles during the process of receptor internalization (See <a class="figpopup" href="/books/NBK66153/figure/ml193.f2/?report=objectonly" target="object" rid-figpopup="figml193f2" rid-ob="figobml193f2">Figure 2</a> on right). This assay technology is commercially marketed as Transfluor® assay by Molecular Devices). Upon activation by ligand binding (lysophosphatidyinositol derivatives), the GPR55 receptor undergoes deactivation or “desensitization” by binding of the β-arrestin protein to the activated receptor. The GPCR-β-arrestin complex internalizes, the ligand is removed and the receptor is recycled back to the cell membrane. Localization of the fluorescently labeled β-arrestin can be monitored by image analysis (<a class="bibr" href="#ml193.r7" rid="ml193.r7">7</a>). Dr. Abood found that transient transfections with unmodified GPR55 resulted in similar functional redistribution (data not shown). The primary screen assay is designed to identify compounds inhibiting GPR55 signaling activated by EC80 concentration of the GPR55 agonist lysophosphatidylinositol (LPI). It is important to note that at an EC80 agonist concentration, the calculated IC50’s of tested compounds are probably 5–8 fold higher than the actual binding affinities K<sub>i</sub> of the compounds.</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml193f2" co-legend-rid="figlgndml193f2"><a href="/books/NBK66153/figure/ml193.f2/?report=objectonly" target="object" title="Figure 2" class="img_link icnblk_img figpopup" rid-figpopup="figml193f2" rid-ob="figobml193f2"><img class="small-thumb" src="/books/NBK66153/bin/ml193f2.gif" src-large="/books/NBK66153/bin/ml193f2.jpg" alt="Figure 2. GPR55 Example Images of Positive and Negative Controls." /></a><div class="icnblk_cntnt" id="figlgndml193f2"><h4 id="ml193.f2"><a href="/books/NBK66153/figure/ml193.f2/?report=objectonly" target="object" rid-ob="figobml193f2">Figure 2</a></h4><p class="float-caption no_bottom_margin">GPR55 Example Images of Positive and Negative Controls. Effect of 25 μM agonist LPI (left panel) compared to DMSO control (right panel) </p></div></div><p>The primary screening protocol is described below.</p></div><div id="ml193.s11"><h5>Assay materials</h5><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t2"><a href="/books/NBK66153/table/ml193.t2/?report=objectonly" target="object" title="Table 2" class="img_link icnblk_img figpopup" rid-figpopup="figml193t2" rid-ob="figobml193t2"><img class="small-thumb" src="/books/NBK66153/table/ml193.t2/?report=thumb" src-large="/books/NBK66153/table/ml193.t2/?report=previmg" alt="Table 2. Reagents used for the uHTS experiments." /></a><div class="icnblk_cntnt"><h4 id="ml193.t2"><a href="/books/NBK66153/table/ml193.t2/?report=objectonly" target="object" rid-ob="figobml193t2">Table 2</a></h4><p class="float-caption no_bottom_margin">Reagents used for the uHTS experiments. </p></div></div></div><div id="ml193.s12"><h5>The following uHTS protocol was implemented</h5><p>Day 1:</p><ol><li class="half_rhythm"><div>45ul of cell suspension (200,000 cells/ml in culture medium) was dispensed in each well of the assay plates using a Wellmate bulk dispenser.</div></li><li class="half_rhythm"><div>Plates are incubated overnight or approximately 20 hours at 37 degree C and 5% CO2.</div></li></ol><p>Day2:</p><ol><li class="half_rhythm"><div>Serum is removed by media aspiration and replacing with 45ul serum-free MEM prior to addition of compounds.</div></li><li class="half_rhythm"><div>Compound addition was done on a Biomek FX with 384-head dispenser (Beckman):</div><ol class="lower-alpha"><li class="half_rhythm"><div>5ul of 100μM compound solution was added to columns 3 through 24 of the assay plates for a final assay compound concentration of 10μM and 0.25% DMSO.</div></li><li class="half_rhythm"><div>5ul of DMSO control (2.5% DMSO) working solution was added to column 1 and 2.</div></li></ol></li><li class="half_rhythm"><div>Plates were preincubated with compounds for 30 minutes at 37 degrees C and 5% CO2.</div></li><li class="half_rhythm"><div>5ul of agonist (50μM LPI) working solution was added to the entire plate except for column 1 for a final compound concentration of 5 μM (~EC80).</div></li><li class="half_rhythm"><div>Plates were incubated for 75 minutes at 37 degrees C and 5% CO2.</div></li><li class="half_rhythm"><div>Media was aspirated leaving 20ul liquid in each well using a Titertek plate washer.</div></li><li class="half_rhythm"><div>40ul of fixative working solution was added to each well using a Wellmate bulk dispenser (Matrix) for a final concentration of 4% PFA.</div></li><li class="half_rhythm"><div>Plates were incubated for 40 minutes at room temperature.</div></li><li class="half_rhythm"><div>Fixative was aspirated and plates were washed twice with 50ul PBS leaving 20ul liquid in each well using a Titertek plate washer.</div></li><li class="half_rhythm"><div>40ul of DAPI working solution was added using a Wellmate bulk dispenser for a final DAPI concentration of 100ng/ml. Aluminum plate sealers were applied to each plate.</div></li></ol><p>Imaging and Analysis:</p><ol><li class="half_rhythm"><div>Image acquisition was performed on an Opera QEHS (Perkin Elmer) with 45 plate capacity loader/stacker and the following settings:</div><ul><li class="half_rhythm"><div>20x 0.45 NA air objective</div></li><li class="half_rhythm"><div>Acquisition camera set to 2-by-2 binning for an image size of 688 by 512 pixels</div></li><li class="half_rhythm"><div>2 channels acquired sequentially: Exp1Cam1 = β-arrestin GFP using 488 nm laser excitation and 540/70 nm emission filters, Exp2Cam2 = DAPI (nuclei) using 365 nm Xenon lamp excitation and 450/50 nm emission filters</div></li><li class="half_rhythm"><div>2 fields per well for Primary screen, 4 fields per well for Hit Confirmation</div></li></ul></li><li class="half_rhythm"><div>Image analysis was performed using the Acapella Spot Detection Algorithm and the following analysis settings:</div><ol class="lower-alpha"><li class="half_rhythm"><div>Nuclei Detection</div><ul><li class="half_rhythm"><div>Threshold Adjustment: 1.5</div></li><li class="half_rhythm"><div>Nuclear Splitting Adjustment: 7</div></li><li class="half_rhythm"><div>Minimum Nuclear Area: 70</div></li><li class="half_rhythm"><div>Minimum Nuclear Contrast: 0.1</div></li></ul></li><li class="half_rhythm"><div>Cytoplasm Detection</div><ul><li class="half_rhythm"><div>Cytoplasm Threshold Adjustment: 0.45</div></li><li class="half_rhythm"><div>Cytoplasm Individual Threshold Adjustment: 0.15</div></li><li class="half_rhythm"><div>Spot Detection</div></li><li class="half_rhythm"><div>Spot Minimum Distance 3</div></li><li class="half_rhythm"><div>Spot Peak Radius 0</div></li><li class="half_rhythm"><div>Spot Reference Radius 2</div></li><li class="half_rhythm"><div>Spot Minimum Contrast 0.25</div></li><li class="half_rhythm"><div>Spot Minimum to Cell Intensity 1</div></li></ul></li></ol></li><li class="half_rhythm"><div>Metrics were calculated from</div><ol class="lower-alpha"><li class="half_rhythm"><div>Nuclei Images:</div><ul><li class="half_rhythm"><div>Cell Count ("NumberofCellsAnalyzed")</div></li><li class="half_rhythm"><div>Nuclei Area ("AreaoftheNucleus")</div></li><li class="half_rhythm"><div>Integrated Intensity of the Nuclei ("TotalIntegratedIntensityoftheNucleus")</div></li><li class="half_rhythm"><div>Average Intensity of the Nuclei ("AverageIntensityoftheNucleus")</div></li></ul></li><li class="half_rhythm"><div>GFP Images:</div><ul><li class="half_rhythm"><div>Integrated Intensity of the Cytoplasm ("TotalCytoplasmIntensity")</div></li><li class="half_rhythm"><div>Integrated Intensity of the Detected Spots ("TotalSpotIntensity")</div></li><li class="half_rhythm"><div>Ratio of the Integrated Spot to Integrated Cytoplasm Intensities ("RatioofSpotIntensitytoCytoplasmintensity")</div></li><li class="half_rhythm"><div>Number of Spots per Cell ("AverageSpotsPerCell")</div></li><li class="half_rhythm"><div>Percentage of Cells Positive for Spot Formation ("PercentagePositiveCells")</div></li></ul></li></ol></li><li class="half_rhythm"><div>Actives from the primary screen were determined using CBIS software (ChemInnovations) by calculating the % Activation of the "AverageSpotsPerCell" metric and using a hit criteria of Activity > 60% and "NumberofCellsAnalyzed" > 100 and “TotalCytoplasmIntensity” < 500,000. Wells with cell counts lower than 100 in the 2 acquired images were flagged "cytotoxic / low cell count". Wells with a very high total GFP intensity (“TotalCytioplasmIntensity” > 500,000) were flagged artifacts due to autofluorescence. All flagged wells were excluded from hit selection.</div></li></ol><p>The primary screen was performed at a compound concentration of 10μM in 384-well format. The average Z′ for the screen was 0.78, the average Signal-To-Background (S/B) was 32.4, Signal-To-Noise was 228, and Signal Window was 12.3 using the average number of spots per cell (“AverageSpotsPerCell”) as the primary assay read-out.</p><p><b><u>Rationale for confirmatory, counter and selectivity assay</u></b> See flowchart below:</p><div id="ml193.fu4" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu4.jpg" alt="Critical Path Flowchart for GPR55 Antagonist Project." /></div><h3><span class="title">Critical Path Flowchart for GPR55 Antagonist Project</span></h3></div><p><b>Assay artifacts – Assay technology</b>: The primary and confirmatory assay is an image-based HCS, which is based on translocation of GFP-labeled β-arrestin from the cytosolic compartment via the plasma membrane to intracellular pits/vesicles upon stimulation. These fluorescent pits/vesicles are detected in the images as small fluorescent aggregates or spots (<a class="bibr" href="#ml193.r14" rid="ml193.r14">14</a>). This assay read-out is highly specific, and the only assay artifact of concern was high autofluorescence of some test compounds. However, as this is an image based assay, the redistribution features could still be visualized for the most part even with high fluorescence backgrounds, which are expected to be isotropic and homogenous. Unlike a plate reader based assay, the algorithms of the HCS device allow rejection of data if not located and registered to a “cell” during image segmentation. Additionally, one of the HCS parameters quantifies the Total (Integrated) Intensity of the Cytoplasm, which is equivalent to an increased intensity in the GFP channel. Since the GFP intensity in this GFP redistribution assay is approximately the same for all cells independent of signaling activation, a significant increase in the total GFP intensity can be used as rejection criterion if there is potentially uncompensatable compound autofluorescence (i.e. TotalCytoplasmIntensity > 500,000 units). Additionally, the image-based high-content nature of the assay allows for monitoring of the number of cells imaged per well to flag wells with low cell counts due to toxicity or liquid handling.</p><p><b>Assay artifacts – Receptor independent signaling:</b> The formal possibility exits where the compounds may act as antagonist, independent of the GPR55 receptor, i.e. acting directly on components downstream of the GPR55 receptor, e.g. at the GFP-β-arrestin trafficking directly. As shown above in our Critical Path Workflow, any such compounds would be rejected as such a mechanism would also activate the other related GPCR receptors e.g. GPR35, CB1, and CB2. These assays are also performed as image-based HCS assays using the same Transfluor™ HCS redistribution assays. These compounds would then be regarded as “promiscuous” and non-selective for GPR55.</p></div></div></div></div><div id="ml193.s13"><h3>Confirmation assays</h3><p>Initial hit confirmation of compound solutions resupplied by the MLSMR was done at a single compound concentration (10 μM) in duplicates using the primary screen assay to confirm activity of the hit compounds. Compounds with confirmed activity at 10 μM were tested in 7-point dose responses (0.5 to 32 μM) to evaluate potency. Potent compounds (IC<sub>50</sub> <5 μM) were clustered into scaffolds and 10-point dose responses (0.06 to 32 μM) were performed for dry powder compounds selected from hits and their commercially available analogs.</p><p>The following are confirmation assays for this project:</p><ul class="simple-list"><li class="half_rhythm"><div><i>Assay 1:</i> Image-based HTS for Selective Antagonists of GPR55 <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2013" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2013</a>)</i></div></li><li class="half_rhythm"><div><i>Assay 2:</i> SAR analysis of selective Antagonists of GPR55 using an Image-Based Assay <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2820" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2820</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485287" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485287</a>)</i></div></li></ul></div><div id="ml193.s14"><h3>Secondary Assays: Counterscreen / Selectivity assays</h3><p>Since GPR55 is thought to be a putative cannabinoid receptor, selectivity against the other cannabinoid receptors (CB1 and CB2) was established using the same image-based high-content assay technology (<a class="bibr" href="#ml193.r13" rid="ml193.r13">13</a>). Additionally, selectivity against the GPR35 receptor was evaluated, since GPR35 and GPR55 share ~30% identity (<a class="bibr" href="#ml193.r8" rid="ml193.r8 ml193.r9 ml193.r10">8–10</a>,<a class="bibr" href="#ml193.r12" rid="ml193.r12">12</a>). In addition to providing a selectivity panel, these assays eliminated false positives caused by assay artifacts, since all selectivity assays utilized the same assay technology. Selectivity Assays were performed in 10-point dose responses (0.06 to 32 μM) for dry powder compounds.</p><p>The following are selectivity assays for this project:</p><ul class="simple-list"><li class="half_rhythm"><div><i>Assay 1:</i> SAR analysis of Agonists of the GPR35 Receptor using an Image-Based Assay <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2809" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2809</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485286" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485286</a>)</i></div></li><li class="half_rhythm"><div><i>Assay 2:</i> SAR analysis of Antagonists of the GPR35 Receptor using an Image-Based Assay <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2815" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2815</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485283" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485283</a>)</i></div></li><li class="half_rhythm"><div><i>Assay 3:</i> SAR analysis of Agonists of the Cannabinoid Receptor 1 using an Image-Based Assay <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2814" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2814</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485282" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485282</a>)</i></div></li><li class="half_rhythm"><div><i>Assay 4:</i> SAR analysis of Antagonists of the Cannabinoid Receptor 1 using an Image-Based Assay <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2822" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2822</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485285" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485285</a>)</i></div></li><li class="half_rhythm"><div><i>Assay 5:</i> SAR analysis of Agonists of the Cannabinoid Receptor 2 using an Image-Based Assay <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2835" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2835</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485277" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485277</a>)</i></div></li><li class="half_rhythm"><div><i>Assay 6:</i> SAR analysis of Antagonists of the Cannabinoid Receptor 2 using an Image-Based Assay <i>(<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2836" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 2836</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485278" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485278</a>)</i></div></li></ul></div><div id="ml193.s15"><h3>Post Probe Characterization Tertiary Assays (Assay Provider)</h3><p>The identified GPR55 antagonist probes and selected analogs are characterized further by two assays performed in the assay provider’s and her collaborator’s labs. The pERK assay evaluates downstream activity in the GPR55 signaling pathway (which can represent either G-protein dependent signaling, or G-protein independent signaling), while the PKC β II translocation assay evaluates a G-protein dependent signaling pathway</p><p>The following are secondary assays for this project:</p><ul class="simple-list"><li class="half_rhythm"><div><i>Assay 1: SAR Analysis for the identification of Selective Antagonists of GPR55 using an Image-Based Screen [PKC β II translocation] (<a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/488947" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID488947</a>)</i></div></li></ul></div></div><div id="ml193.s16"><h2 id="_ml193_s16_">2. Center Summary of Results</h2><p>The following flowchart summarizes the compound triage and decision tree for advancement of compounds:</p><p>291,669 compounds from the NIH MLSMR collection (this was the full size of the library at the time of this HCS) were tested in the image-based GPR55 HCS β-Arrestin primary screen. The primary screen resulted in 805 compounds that were considered as hits using the hit criterion of >60% activity as compared to the negative control LPI (lysophosphatidylinositol) and the positive control of the positive control of vehicle (DMSO) only. Additionally, hit compounds had to meet the criterion of >100 cells in the imaged area of the well and total cytoplasmic intensity of < 500,000 relative intensity units. Cherry picks of the identified 805 compounds were ordered from DPI for hit confirmation.</p><p>Liquid reorders of 705 compounds were received from DPI in 100% DMSO. A single-concentration (10 μM) confirmatory antagonist assay was performed in duplicate. 273 compounds reconfirmed and were evaluated for potency using a 7-point dose response ranging from 32 μM to 500 nM, in duplicate, on 2 different days. In the dose response experiments, 39 compounds resulted in an IC<sub>50</sub> of >1 μM and 228 compounds had an IC<sub>50</sub> of 1–10 μM. The maximum-common-substructure-based clustering analysis of 273 compounds resulted in 52 scaffolds and 54 singletons. By analyzing assay data in terms of scaffolds, 44 compounds from 17 scaffolds were selected for follow-up studies. Only 38 of these 44 compounds were readily available as dry powders.</p><p>The reordered 38 compounds as well as 67 subsequently ordered analog-by-catalog (ABC) compounds were run in the GPR55 antagonist assay using 10-point dose responses ranging from 32μM to 62nM. 23 of the compounds showed inhibition with I IC<sub>50</sub> < 1 μM. The dry powder compounds were also evaluated for selectivity in the following selectivity assays: CB1 agonist, CB1 antagonist, CB2 agonist, CB2 antagonist, GPR35 agonist, and GPR35 antagonist assays. 20 of the compounds with GPR55 IC<sub>50</sub> < 5μM were not completely selective: 2 compounds showed less than 10-fold selectivity against GPR35 agonist, and 18 had less than 10-fold selectivity against CB1 antagonist. Out of these 18 compounds 3 were also not selective against CB2 antagonist. All other potent compounds were > 10-fold selective against the tested panel.</p><div id="ml193.fu5" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu5.jpg" alt="GPR55 Antagonist Compound Flowchart." /></div><h3><span class="title">GPR55 Antagonist Compound Flowchart</span></h3></div></div><div id="ml193.s17"><h2 id="_ml193_s17_">3. Probe Optimization</h2><div id="ml193.s18"><h3>i. Describe SAR & chemistry strategy (including structure and data) that led to the probe</h3><p>The SAR for probe CID1261822 can be seen in <a class="figpopup" href="/books/NBK66153/table/ml193.t3/?report=objectonly" target="object" rid-figpopup="figml193t3" rid-ob="figobml193t3">Table 3</a>. This probe series consists of a quinoline core attached to an aryl sulfonamide via an amide linkage. The preferred groups at R<sub>1</sub> are 5- or 6-membered heterocycles. At R<sub>2</sub>, both aryl and heteroaryl substitutents are tolerated. Phenyl substituents at this position can be substituted at either the <i>ortho</i> or <i>para</i> positions. R<sub>3</sub> can tolerate either methyl or hydrogen substitution. As can be seen in the table, the preferred heterocycle at R<sub>1</sub> is an isoxazole. The presence of this moiety at R<sub>1</sub> resulted in the 3 most potent GPR55 antagonists, CIDs 1261822, 1261290, and 1261872. All 3 of these compounds have GPR55 antagonist IC<sub>50</sub> values less than 1 μM and are completely selective over the cannabinoid receptors 1 and 2. The isoxazole subclass is also represented in 2 less potent representatives, CIDs 2333197 and 1011349, the latter of which had essentially no inhibitory activity. It can be noted in these 2 examples the R<sub>3</sub> substituent was H, whereas in the very potent isoxazoles, R<sub>3</sub> is methyl substituted. This underscores the importance of the R<sub>3</sub> substitution in this subclass. Some of the other tolerated heterocycles at R<sub>1</sub> include thiazoles and pyrimidines. However, both of these groups resulted in analogues with significantly less GPR55 antagonist potency. Finally, it was noted that R<sub>2</sub> substitution preferred the 2- and 3-pyridyl substitution. Deviation from these preferred substituents, including the 4-pyridyl as in CID2385808, resulted in a dramatic loss of activity.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t3"><a href="/books/NBK66153/table/ml193.t3/?report=objectonly" target="object" title="Table 3" class="img_link icnblk_img figpopup" rid-figpopup="figml193t3" rid-ob="figobml193t3"><img class="small-thumb" src="/books/NBK66153/table/ml193.t3/?report=thumb" src-large="/books/NBK66153/table/ml193.t3/?report=previmg" alt="Table 3. SAR of GPR55 Antagonist Probe ML193 (#1): CID1261822." /></a><div class="icnblk_cntnt"><h4 id="ml193.t3"><a href="/books/NBK66153/table/ml193.t3/?report=objectonly" target="object" rid-ob="figobml193t3">Table 3</a></h4><p class="float-caption no_bottom_margin">SAR of GPR55 Antagonist Probe ML193 (#1): CID1261822. </p></div></div><p>The SAR for probe CID1434953 can be seen in <a class="figpopup" href="/books/NBK66153/table/ml193.t4/?report=objectonly" target="object" rid-figpopup="figml193t4" rid-ob="figobml193t4">Table 4</a>. This series consists of a substituted thienopyrimidine core containing the R<sub>1</sub> and R<sub>2</sub> substituents and an acylated piperazine containing R<sub>3</sub>. It is clear from the SAR table that the preferred R<sub>1</sub> group is a methyl. However, there are limited examples of a small cycloalkane being tolerated at this position, as can be seen in CID655864. Preference for R<sub>2</sub> being furan is also clear from the data in the SAR table. When this group is substituted with aromatic or alkyl groups as in CIDs 1434956 or 1434957, respectively, a dramatic loss in GPR55 antagonist potency is seen. Another interesting observation is that when the furan ring is saturated to a tetrahydrofuran, as in CID4877555, activity is abolished as well. Finally, substitution on the core thienopyrimidine ring was explored. While majority of the derivatives possessed an unsubstituted fused tetrahydropyranyl ring, methyl substitution of this ring, as in CIDs 3193014 and 3193022, was also tolerated, resulting in potent, selective antagonists.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t4"><a href="/books/NBK66153/table/ml193.t4/?report=objectonly" target="object" title="Table 4" class="img_link icnblk_img figpopup" rid-figpopup="figml193t4" rid-ob="figobml193t4"><img class="small-thumb" src="/books/NBK66153/table/ml193.t4/?report=thumb" src-large="/books/NBK66153/table/ml193.t4/?report=previmg" alt="Table 4. SAR of GPR55 Antagonist Probe ML192 (#2): CID1434953." /></a><div class="icnblk_cntnt"><h4 id="ml193.t4"><a href="/books/NBK66153/table/ml193.t4/?report=objectonly" target="object" rid-ob="figobml193t4">Table 4</a></h4><p class="float-caption no_bottom_margin">SAR of GPR55 Antagonist Probe ML192 (#2): CID1434953. </p></div></div><p>The SAR for probe molecule CID23612552 is seen in <a class="figpopup" href="/books/NBK66153/table/ml193.t5/?report=objectonly" target="object" rid-figpopup="figml193t5" rid-ob="figobml193t5">Table 5</a>. This class consists of piperadinyloxadiazolone core substituted with various phenylacetyl groups on a piperdine ring. The core molecule with a phenyl group in the 5-position of the oxadiazolone ring is conserved throughout the derivatives, indicating a tight yet tractable SAR in this series. The piperidine portion of this probe series tolerates a variety of groups having a specific motif consisting of a substituted phenylacetyl group. The benzylic methylene of this group can be sequentially substituted up to a quaternary center, with these derivatives yielding the most potent analogues, including the probe molecule. Of the derivatives possessing this quaternary substitution at the benzylic position, the cyclopropyl analogues clearly afford the most potent derivatives. This is most likely due to the bond angle difference that is seen in divergence from the tetrahedral geometry as in the hydrogen or monosubstituted species, both of which are less potent (e.g. CID23612541). This may also be due to secondary orbital interactions with the adjacent aromatic groups as cyclopropyl bonds have more p character. Substitution of the aromatic portion of the phenylacetyl group is also important. Comparison of the probe CID23612552 to a similar compound that lacks the tolyl substitution, such as CID23612554, leads to a dramatic loss of antagonist potency. Finally, it can be noted that various derivatives show CB1 antagonist activity, with CID23612543 being a potent CB1 antagonist. It can be noted that this molecule bears a spirocyclopentyl group at the benzylic position. The probe molecule, however, is devoid of any CB activity. It appears that the cyclopropyl group lends additional selectivity for GPR55 over the CB receptors.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t5"><a href="/books/NBK66153/table/ml193.t5/?report=objectonly" target="object" title="Table 5" class="img_link icnblk_img figpopup" rid-figpopup="figml193t5" rid-ob="figobml193t5"><img class="small-thumb" src="/books/NBK66153/table/ml193.t5/?report=thumb" src-large="/books/NBK66153/table/ml193.t5/?report=previmg" alt="Table 5. SAR of GPR55 Antagonist Probe ML191 (#3): CID23612552." /></a><div class="icnblk_cntnt"><h4 id="ml193.t5"><a href="/books/NBK66153/table/ml193.t5/?report=objectonly" target="object" rid-ob="figobml193t5">Table 5</a></h4><p class="float-caption no_bottom_margin">SAR of GPR55 Antagonist Probe ML191 (#3): CID23612552. </p></div></div></div></div><div id="ml193.s19"><h2 id="_ml193_s19_">4. Probe</h2><div id="ml193.s20"><h3>a. Chemical name of probe compounds <i>(from PubChem)</i></h3><p><b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a> - CID1261822</b> is <i>N</i>-(4-(<i>N</i>-(3,4-dimethylisoxazol-5yl)sulfamoyl)-phenyl)-6,8-dimethyl-2-(pyridin-2-yl)quinoline-4-carboxamide</p><p><b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a> - CID1434953</b> is furan-2-yl(4-(2-methyl-5,6,7,8-tetrahydro-benzo[4,5]thieno[2,3-d]pyrimidin-4-yl)piperazin-1-yl)methanone</p><p><b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a> - CID23612552</b> is 5-phenyl-3-(1-(1-(<i>p</i>-tolyl)cyclopropane-carbonyl)piperidin-4-yl)-1,3,4-oxadiazol-2(3<i>H</i>)-one</p></div><div id="ml193.s21"><h3>b. Probe chemical structure including stereochemistry if known</h3><div id="ml193.fu6" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu59.jpg" alt="ML 193." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 193</a></span></h3><div class="caption"><p>CID 1261822</p></div></div><div id="ml193.fu7" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu60.jpg" alt="ML 192." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 192</a></span></h3><div class="caption"><p>CID 1434953</p></div></div><div id="ml193.fu8" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu61.jpg" alt="ML 191." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 191</a></span></h3><div class="caption"><p>CID 23612552</p></div></div></div><div id="ml193.s22"><h3>c. Structural Verification Information of probe SID</h3><p><b>Probe</b>
|
|
<b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a> - CID1261822</b> corresponds to <a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID 87225762</a></p><p><b>Purity:</b> >98% (HPLC)</p><p><b>Mass Spec:</b> HRMS (ESI) <i>m/z</i> calcd for C<sub>28</sub>H<sub>25</sub>N<sub>5</sub>O<sub>4</sub>S ([M+H]<sup>+</sup>), 528, found 528.</p><div id="ml193.fu9" class="figure"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu62.jpg" alt="Image ml193fu62" /></div></div><div id="ml193.fu10" class="figure"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu63.jpg" alt="Image ml193fu63" /></div></div><div id="ml193.fu11" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu64.jpg" alt="1H NMR." /></div><h3><span class="title"><sup>1</sup>H NMR</span></h3></div><p><b>PROBE</b>
|
|
<b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a> - CID1434953.</b> The probe SID is <a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225747</a></p><p><b>Purity:</b> >98% (HPLC)</p><p><b>Mass Spec:</b> HRMS (ESI) <i>m/z</i> calcd for C<sub>20</sub>H<sub>22</sub>N<sub>4</sub>O<sub>2</sub>S ([M+H]<sup>+</sup>), 383, found 383.</p><div id="ml193.fu12" class="figure"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu65.jpg" alt="Image ml193fu65" /></div></div><div id="ml193.fu13" class="figure"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu66.jpg" alt="Image ml193fu66" /></div></div><div id="ml193.fu14" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu67.jpg" alt="1H NMR." /></div><h3><span class="title"><sup>1</sup>H NMR</span></h3></div><p><b>PROBE</b>
|
|
<b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a> - CID23612552.</b> The probe SID is <a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225750</a></p><p><b>Purity:</b> >98% (HPLC)</p><p><b>Mass Spec:</b> HRMS (ESI) <i>m/z</i> calcd for C<sub>24</sub>H<sub>25</sub>N<sub>3</sub>O<sub>3</sub> ([M+H]<sup>+</sup>), 404, found 404.</p><div id="ml193.fu15" class="figure"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu68.jpg" alt="Image ml193fu68" /></div></div><div id="ml193.fu16" class="figure"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu69.jpg" alt="Image ml193fu69" /></div></div><div id="ml193.fu17" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu70.jpg" alt="1H NMR." /></div><h3><span class="title"><sup>1</sup>H NMR</span></h3></div></div><div id="ml193.s23"><h3>d. If available from a vendor, please provide details</h3><p>PubChem CID1261822 is available from Chembridge # 7924036</p><p>PubChem CID1434953 is available from Asinex # ASN 04888279</p><p>PubChem CID23612552 is available from ChemDiv # D327-0013</p></div><div id="ml193.s24"><h3>e. Provide MLS# that verifies the submission of probe molecule and five related samples that were submitted to the SMR collection</h3><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t6"><a href="/books/NBK66153/table/ml193.t6/?report=objectonly" target="object" title="Table 6" class="img_link icnblk_img figpopup" rid-figpopup="figml193t6" rid-ob="figobml193t6"><img class="small-thumb" src="/books/NBK66153/table/ml193.t6/?report=thumb" src-large="/books/NBK66153/table/ml193.t6/?report=previmg" alt="Table 6. Probe Submission for GPR55 Antagonist Probes." /></a><div class="icnblk_cntnt"><h4 id="ml193.t6"><a href="/books/NBK66153/table/ml193.t6/?report=objectonly" target="object" rid-ob="figobml193t6">Table 6</a></h4><p class="float-caption no_bottom_margin">Probe Submission for GPR55 Antagonist Probes. </p></div></div></div><div id="ml193.s25"><h3>f. Describe mode of action for biological activity of probe</h3><p>The identified GPR55 antagonists appear to selectively inhibit GPR55 receptor signaling, but do not inhibit the related cannabinoid receptors 1 and 2 nor the related GPR35 orphan receptor. Since the selectivity assays used the same β-arrestin HCS assay technology and the probes did not inhibit non-selectively, they are not non-specifically inhibiting signaling at or down-stream of the β-arrestin pathway. Since the HCS assay technology measures β-arrestin-GPCR complex internalization into clathrin-coated pits within the cell, the identified antagonist compounds have to inhibit pathway signaling at the β-arrestin-GPCR complex internalization or any process upstream. In tertiary studies by Drs. Abood (still ongoing) and Barak, these probes did show inhibition of ERK1/2 phosphorylation as well as inhibition of PKCβII translocation, confirming the downstream biological response of the compounds inhibitory activities.</p><div id="ml193.s26"><h4>Dose response for 3 GPR55 antagonist probes</h4><div id="ml193.fu18" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu71.jpg" alt="Dose response curves for the 3 probes for the GPR55 antagonist assay and all selectivity assays." /></div><h3><span class="title">Dose response curves for the 3 probes for the GPR55 antagonist assay and all selectivity assays</span></h3><div class="caption"><p>% Efficacy is reported representing either % Inhibition (antagonist assays) or % Activation (agonist assays) as compared to control compounds specific to each assay (refer to PubChem AIDs for details)</p></div></div><p>This project is judged as highly successful, since the antagonists described in and derived from this project provide “clean” tool compounds with which to evaluate GPR55 signaling in cells and in the future in animals. A recent, March 30, 2011 SciFinder search by Dr. Dahl did not find any reports of antagonists for GPR55.</p></div></div><div id="ml193.s27"><h3>g. Detailed synthetic pathway for making probe</h3><p><a class="figpopup" href="/books/NBK66153/figure/ml193.f3/?report=objectonly" target="object" rid-figpopup="figml193f3" rid-ob="figobml193f3">Scheme 1 (a</a> – <a class="figpopup" href="/books/NBK66153/figure/ml193.f5/?report=objectonly" target="object" rid-figpopup="figml193f5" rid-ob="figobml193f5">c</a>). Synthetic routes for the three (3) probe molecules are shown below:</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml193f3" co-legend-rid="figlgndml193f3"><a href="/books/NBK66153/figure/ml193.f3/?report=objectonly" target="object" title="1a" class="img_link icnblk_img figpopup" rid-figpopup="figml193f3" rid-ob="figobml193f3"><img class="small-thumb" src="/books/NBK66153/bin/ml193f3.gif" src-large="/books/NBK66153/bin/ml193f3.jpg" alt="1a" /></a><div class="icnblk_cntnt" id="figlgndml193f3"><h4 id="ml193.f3"><a href="/books/NBK66153/figure/ml193.f3/?report=objectonly" target="object" rid-ob="figobml193f3">1a</a></h4></div></div><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml193f4" co-legend-rid="figlgndml193f4"><a href="/books/NBK66153/figure/ml193.f4/?report=objectonly" target="object" title="1b" class="img_link icnblk_img figpopup" rid-figpopup="figml193f4" rid-ob="figobml193f4"><img class="small-thumb" src="/books/NBK66153/bin/ml193f4.gif" src-large="/books/NBK66153/bin/ml193f4.jpg" alt="1b" /></a><div class="icnblk_cntnt" id="figlgndml193f4"><h4 id="ml193.f4"><a href="/books/NBK66153/figure/ml193.f4/?report=objectonly" target="object" rid-ob="figobml193f4">1b</a></h4></div></div><div class="iconblock whole_rhythm clearfix ten_col fig" id="figml193f5" co-legend-rid="figlgndml193f5"><a href="/books/NBK66153/figure/ml193.f5/?report=objectonly" target="object" title="1c" class="img_link icnblk_img figpopup" rid-figpopup="figml193f5" rid-ob="figobml193f5"><img class="small-thumb" src="/books/NBK66153/bin/ml193f5.gif" src-large="/books/NBK66153/bin/ml193f5.jpg" alt="1c" /></a><div class="icnblk_cntnt" id="figlgndml193f5"><h4 id="ml193.f5"><a href="/books/NBK66153/figure/ml193.f5/?report=objectonly" target="object" rid-ob="figobml193f5">1c</a></h4></div></div><p><i>Synthesis of</i>
|
|
<b><i><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></i></b><i> (<a class="figpopup" href="/books/NBK66153/figure/ml193.f3/?report=objectonly" target="object" rid-figpopup="figml193f3" rid-ob="figobml193f3">Scheme 1a</a>)</i>: To a solution of <i>tert</i>-butyl (4-(chlorosulfonyl)phenyl)carbamate (145 mg, 0.5 mmol) and <i>N</i>,<i>N</i>-diisopropylethylamine (1.5 eq.) in dichloromethane (5 mL) was added 3,4-dimethylisoxazol-5-amine. This mixture was stirred at rt overnight. After evaporation of solvent and purification by flash chromatography, the residue was suspended in a 20% solution of trifluoroacetic acid in dichloromethane. Removal of solvent and excess acid afforded the sulfonamide intermediate which was treated with 6,8-dimethyl-2-(pyridin-2-yl)quinoline-4-carbonyl chloride (148 mg, 0.5 mmol) in the presence of <i>N</i>,<i>N</i>-diisopropylethylamine (2 eq.) in dichloromethane. After 3 h at rt, the solvent was removed by rotary evaporation and <a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a> was isolated by reverse-phase preparative HPLC as a white solid.</p><p><i>Synthesis of</i>
|
|
<b><i><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></i></b><i> (<a class="figpopup" href="/books/NBK66153/figure/ml193.f3/?report=objectonly" target="object" rid-figpopup="figml193f3" rid-ob="figobml193f3">Scheme 1a</a>): N</i>-Boc-piperazine (93 mg, 0.5 mmol) was treated with furan-2-carbonyl chloride (65 mg, 0.5 mmol) and <i>N</i>,<i>N</i>-diisopropylethylamine (1.5 eq.) in dichloromethane. After stirring for 1 h, the acylated boc-piperazine intermediate was isolated by silica gel flash chromatography. Removal of the boc group with TFA (20% in dichloromethane, 10 min) followed by evaporation and treatment with 4-chloro-2-methyl-5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-<i>d</i>]pyrimidine (119 mg, 0.5 mmol) and <i>N</i>,<i>N</i>-diisopropylethylamine (2 eq.) in dichloromethane for 5 h afforded <a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 192</a> which was isolated as a white solid by reverse-phase preparative HPLC.</p><p><i>Synthesis of</i>
|
|
<b><i><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></i></b><i> (<a class="figpopup" href="/books/NBK66153/figure/ml193.f3/?report=objectonly" target="object" rid-figpopup="figml193f3" rid-ob="figobml193f3">Scheme 1a</a>)</i>: Benzohydrazide (136 mg, 1.0 mmol) was treated with bis(trichloromethyl) carbonate (2 eq.) in pyridine at 0 °C. After slow warm to rt, the mixture was stirred for an additional 20 h. After evaporation of solvent, the oxadiazolone product was suspended in a 1% acetic acid in dimethylformamide solution and treated with <i>N</i>-boc-piperidinone (1.2 eq.) and sodium cyanoborohydride (2 eq.). This mixture was stirred at rt for 16 h after which the residue was diluted with water and extracted (ethyl acetate). Evaporation of the organic layer and re-suspension in a 20% solution of trifluoroacetic acid in THF afforded the free amine which, after evaporation of excess acid and solvent, was treated with 1-(<i>p</i>-tolyl)cyclopropanecarbonyl chloride (1.5 eq.) and <i>N</i>,<i>N</i>-diisopropylethylamine (2 eq.) in dichloromethane for 5 h to afford <a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 191</a> which was isolated by reverse-phase preparative HPLC.</p></div><div id="ml193.s28"><h3>h. Center summary of probe properties (solubility, absorbance/fluorescence, reactivity, toxicity, etc</h3><p>These probes have been profiled for their <i>in vitro</i> ADME/T properties (see <a class="figpopup" href="/books/NBK66153/table/ml193.t7/?report=objectonly" target="object" rid-figpopup="figml193t7" rid-ob="figobml193t7">Table 7</a>). They all display low to moderate aqueous solubility at various pH levels and lower solubility in PBS versus phosphate-free buffer. The apparent stability of the probe in PBS was initially thought to be poor, however, it was ascertained that their low aqueous solubility confounded this evaluation (data not shown). <b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b>, <b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b> and <b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b> were completely stable for up to 48 hrs in 1:1 acetonitrile:PBS, as seen in figures below.</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t7"><a href="/books/NBK66153/table/ml193.t7/?report=objectonly" target="object" title="Table 7" class="img_link icnblk_img figpopup" rid-figpopup="figml193t7" rid-ob="figobml193t7"><img class="small-thumb" src="/books/NBK66153/table/ml193.t7/?report=thumb" src-large="/books/NBK66153/table/ml193.t7/?report=previmg" alt="Table 7. Summary of in vitro ADMET/PK Properties of GP55 Agonist Probe(s)." /></a><div class="icnblk_cntnt"><h4 id="ml193.t7"><a href="/books/NBK66153/table/ml193.t7/?report=objectonly" target="object" rid-ob="figobml193t7">Table 7</a></h4><p class="float-caption no_bottom_margin">Summary of <i>in vitro</i> ADMET/PK Properties of GP55 Agonist Probe(s). </p></div></div><div id="ml193.fu19" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu72.jpg" alt="ML193 Stability in 1:1 PBS/ACN." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a> Stability in 1:1 PBS/ACN</span></h3></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193tu2"><a href="/books/NBK66153/table/ml193.tu2/?report=objectonly" target="object" title="Table" class="img_link icnblk_img figpopup" rid-figpopup="figml193tu2" rid-ob="figobml193tu2"><img class="small-thumb" src="/books/NBK66153/table/ml193.tu2/?report=thumb" src-large="/books/NBK66153/table/ml193.tu2/?report=previmg" alt="Image " /></a><div class="icnblk_cntnt"><h4 id="ml193.tu2"><a href="/books/NBK66153/table/ml193.tu2/?report=objectonly" target="object" rid-ob="figobml193tu2">Table</a></h4></div></div><div id="ml193.fu20" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu73.jpg" alt="ML192 Stability in 1:1 PBS/ACN." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a> Stability in 1:1 PBS/ACN</span></h3></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193tu3"><a href="/books/NBK66153/table/ml193.tu3/?report=objectonly" target="object" title="Table" class="img_link icnblk_img figpopup" rid-figpopup="figml193tu3" rid-ob="figobml193tu3"><img class="small-thumb" src="/books/NBK66153/table/ml193.tu3/?report=thumb" src-large="/books/NBK66153/table/ml193.tu3/?report=previmg" alt="Image " /></a><div class="icnblk_cntnt"><h4 id="ml193.tu3"><a href="/books/NBK66153/table/ml193.tu3/?report=objectonly" target="object" rid-ob="figobml193tu3">Table</a></h4></div></div><div id="ml193.fu21" class="figure bk_fig"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu74.jpg" alt="ML191 Stability in 1:1 PBS/ACN." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a> Stability in 1:1 PBS/ACN</span></h3></div><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193tu4"><a href="/books/NBK66153/table/ml193.tu4/?report=objectonly" target="object" title="Table" class="img_link icnblk_img figpopup" rid-figpopup="figml193tu4" rid-ob="figobml193tu4"><img class="small-thumb" src="/books/NBK66153/table/ml193.tu4/?report=thumb" src-large="/books/NBK66153/table/ml193.tu4/?report=previmg" alt="Image " /></a><div class="icnblk_cntnt"><h4 id="ml193.tu4"><a href="/books/NBK66153/table/ml193.tu4/?report=objectonly" target="object" rid-ob="figobml193tu4">Table</a></h4></div></div><p>One of the favorable factors is that the permeability of all three scaffolds is high, indicative of being readily absorbed. While all three probe compounds are stable in plasma, <b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b> and <b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b> are degraded by liver enzymes, though <b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b> is less sensitive with 50–60% remaining after 1 hr. While this may be an issue for oral dosing, other routes of administration may provide significant systemic levels. Finally, <b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b> and <b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b> showed no significant hepatocyte toxicity <i>in vitro</i>, while <b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b> had some low hepatic toxicity.</p><p>The oxadiazolone carbonyl of <b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b> may be reactive to nucleophiles and thus might be an acylating agent. However, we have determined that is NOT reactive to glutathione (see below). The chemical structures of <b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b> and <b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b><b>,</b> in contrast, do not possess any potentially reactive chemical moieties.</p><p><b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a> Glutathione conjugation assay.</b> To assess the potential of the <b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b> for covalent modification, the glutathione transferase activity of the probe compound in the rat hepatic S9 fraction was measured. The S9-based GSH transferase assay has been shown to be a reliable means of identifying compounds known to react with GSH. Briefly, compounds were incubated at 10 μM in PBS buffer with 2 mg/ml rat hepatic S9 fraction and 10 mM glutathione at 37°C for 1.5 hours. The incubation was stopped by protein precipitation using acetonitrile. After drying down of the supernatant, the residues were reconstituted and analyzed by positive-ion electrospray LCMS and analyzed for the presence of any glutathione conjugates. The LCMS data after 1.5 hours showed 96% of unchanged probe compound, indicating a low propensity for covalent modification. <u>In addition, none of the expected GSH conjugate masses were detected.</u> The compounds were tested in duplicate along with the positive control diclofenac, a known GSH conjugator, which showed the expected conjugate masses.</p><div id="ml193.fu22" class="figure"><div class="graphic"><img src="/books/NBK66153/bin/ml193fu75.jpg" alt="Image ml193fu75" /></div></div><p><b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 191</a> Glutathione Reactivity Assessment:</b>
|
|
<b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b> was incubated with glutathione and rat S9 fraction for 1.5 hours. (b) HPLC-MS analysis showed 96.0% of the parent compound remained after 1.5 hours. Also, no GSH adduct masses were detecte</p><p>These probe molecules have demonstrated potent and selective antagonist activity in the image-based primary assay for inhibition of β-arrestin mediated signaling of the GPR55 orphan receptor, with selectivity over the GPR35, cannabinoid CB1 and CB2 receptors.</p></div><div id="ml193.s29"><h3>i. A tabular presentation summarizing known probe properties <i>(computed properties, must match from PubChem)</i></h3><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193tu5"><a href="/books/NBK66153/table/ml193.tu5/?report=objectonly" target="object" title="Table" class="img_link icnblk_img figpopup" rid-figpopup="figml193tu5" rid-ob="figobml193tu5"><img class="small-thumb" src="/books/NBK66153/table/ml193.tu5/?report=thumb" src-large="/books/NBK66153/table/ml193.tu5/?report=previmg" alt="Image " /></a><div class="icnblk_cntnt"><h4 id="ml193.tu5"><a href="/books/NBK66153/table/ml193.tu5/?report=objectonly" target="object" rid-ob="figobml193tu5">Table</a></h4></div></div></div></div><div id="ml193.s30"><h2 id="_ml193_s30_">5. Comparative data showing probe specificity for target in biologically relevant assays</h2><p>Further follow-up studies in the assay provider’s laboratories (Dr. Abood and collaborator Dr. Barak) aimed to validate the biological activities in the signaling pathway downstream of β-arrestin are ongoing. These downstream assays include probe characterization for ERK1/2 activity in GPR55-overexpressing cells and PKC βII translocation in cells with wild-type GPR55 receptor and the assays are described below. The SAR data for the PKC βII translocation assay as well as preliminary data for the pERK assay are summarized for the 3 probes <b>(</b><a class="figpopup" href="/books/NBK66153/table/ml193.t8/?report=objectonly" target="object" rid-figpopup="figml193t8" rid-ob="figobml193t8">Table 9</a>).</p><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figml193t8"><a href="/books/NBK66153/table/ml193.t8/?report=objectonly" target="object" title="Table 9" class="img_link icnblk_img figpopup" rid-figpopup="figml193t8" rid-ob="figobml193t8"><img class="small-thumb" src="/books/NBK66153/table/ml193.t8/?report=thumb" src-large="/books/NBK66153/table/ml193.t8/?report=previmg" alt="Table 9. Downstream assays by Assay Provider." /></a><div class="icnblk_cntnt"><h4 id="ml193.t8"><a href="/books/NBK66153/table/ml193.t8/?report=objectonly" target="object" rid-ob="figobml193t8">Table 9</a></h4><p class="float-caption no_bottom_margin">Downstream assays by Assay Provider. </p></div></div><p><i><u>Assay for ERK1/2 Inhibition in GPR55-Overexpressing Cells (AID Pending):</u></i> This assay was performed by the assay provider to characterize inhibition of the downstream ERK1/2 phosphorylation activity of the probe compounds. It is a Western Blot assay that utilizes a cell line permanently expressing a β-arrestin GFP biosensor and human GPR55 receptor. Western blots were necessary because the known agonist, LPI, stimulates ERK only modestly, thus “In-Cell” <i>in situ</i> Westerns were not feasible in this case Upon agonist-mediated GPCR activation, ERK1/2 phosphorylation occurs as measured by pERK1/2 antibodies. The inhibition ability of the probe compound was measured by quantifying the ERK1/2 phosphorylation activity in the presence of agonist. Because of the low-throughput of the manual Western Blot assay, only probe candidates were tested. In the preliminary data, all probes are showing measureable downstream activities for ERK1/2 phosphorylation.</p><p><u><i>Assay for PKC βII Translocation (</i><i><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/488947" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 488947</a></i><i>) in cells with wild-type GPR55 receptor:</i></u> This imaging assay utilizes HEK293 transiently expressing a PKC βII GFP biosensor and a wild type GPR55 receptor. Upon agonist treatment, the GPR55-mediated G-protein signaling is measured by PKC βII GFP recruitment to the plasma membrane and formation of widespread plasma membrane remodeling. This PKC recruitment is shown as increased fluorescence on plasma membrane and development of blebs. The inhibitory effect of the probe compounds was determined by quantifying inhibition of the PKC βII GFP recruitment to the plasma membrane and formation of widespread plasma membrane remodeling in the presence of agonist. Because of the manual image analysis, results were binned into 5 categories (none, ~1%, ~10%, ~30%, ~60% GFP membrane recruitment/blebs formation) and only probe candidates were tested at 4 concentrations. All probes showed significant inhibition of membrane recruitment/bleb formation indicating inhibition of activity in the signaling pathway.</p><div id="ml193.s31"><h3>Future Studies</h3><p>GPR55 has been implicated in behavior, inflammatory disease, neuropathic pain, osteoporosis and cancer. All these avenues will also be pursued using these novel and potent antagonists. Initially, we plan to use these probes to explore the role of GPR55 though inhibition of its function in a number of <i>in vitro</i> systems where endogenous GPR55 is expressed, such as primary neuronal and glial cultures, neuronal cell lines, and endothelial and bone cells (where solubility is less of an issue). Additionally, GPR55 is expressed in immune cells and this is another area of interest. . For <i>in vivo</i> modeling, we are preparing to assess some of the novel GPR55 agonists in standard behavioral paradigms used in addiction studies, and this will also require assessment of their ability to maintain adequate levels in the central nervous system. CNS active GPR55 antagonists resulting from these studies would have a prominent role in validating the selective behavioral effects of the recently identified GPR55 agonists as well as confirming the off target signaling of CB1 and 2 ligands.</p></div></div><div id="ml193.rl1"><h2 id="_ml193_rl1_">Bibliography</h2><dl class="temp-labeled-list"><dl class="bkr_refwrap"><dt>1.</dt><dd><div class="bk_ref" id="ml193.r1">Andradas C, Caffarel MM, Perez-Gomez E, Salazar M, Lorente M, Velasco G, Guzman M, Sanchez C. The orphan G protein-coupled receptor GPR55 promotes cancer cell proliferation via ERK. <span><span class="ref-journal">Oncogene. </span>2011;<span class="ref-vol">30</span>:245–52.</span> [Epub 2010 Sep. 6] [<a href="https://pubmed.ncbi.nlm.nih.gov/20818416" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 20818416</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>2.</dt><dd><div class="bk_ref" id="ml193.r2">Ford LA, Roelofs AJ, Anavi-Goffer S, Mowat L, Simpson DG, Irving AJ, Rogers MJ, Rajnicek AM, Ross RA. A role for L-alpha-lysophosphatidylinositol and GPR55 in the modulation of migration, orientation and polarization of human breast cancer cells. <span><span class="ref-journal">Br J Pharmacol. </span>2010;<span class="ref-vol">160</span>:762–71.</span> [<a href="/pmc/articles/PMC2931574/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2931574</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/20590578" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 20590578</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>3.</dt><dd><div class="bk_ref" id="ml193.r3">Pineiro R, Maffucci T, Falasca M. The putative cannabinoid receptor GPR55 defines a novel autocrine loop in cancer cell proliferation. <span><span class="ref-journal">Oncogene. </span>2010;<span class="ref-vol">30</span>:142–52.</span> [Epub 2010 Sep 13] [<a href="https://pubmed.ncbi.nlm.nih.gov/20838378" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 20838378</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>4.</dt><dd><div class="bk_ref" id="ml193.r4">Sharir H, Abood ME. Pharmacological characterization of GPR55, a putative cannabinoid receptor. <span><span class="ref-journal">Pharmacol Ther. </span>2010;<span class="ref-vol">126</span>:301–313.</span> [<a href="/pmc/articles/PMC2874616/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2874616</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/20298715" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 20298715</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>5.</dt><dd><div class="bk_ref" id="ml193.r5">Staton PC, Hatcher JP, Walker DJ, Morrison AD, Shapland EM, Hughes JP, Chong E, Mander PK, Green PJ, Billinton A, Fulleylove M, Lancaster HC, Smith JC, Bailey LT, Wise A, Brown AJ, Richardson JC, Chessell IP. The putative cannabinoid receptor GPR55 plays a role in mechanical hyperalgesia associated with inflammatory and neuropathic pain. <span><span class="ref-journal">Pain. </span>2008;<span class="ref-vol">139</span>:225–236.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/18502582" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 18502582</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>6.</dt><dd><div class="bk_ref" id="ml193.r6">Whyte LS, Ryberg E, Sims NA, Ridge SA, Mackie K, Greasley PJ, Ross RA, Rogers MJ. The putative cannabinoid receptor GPR55 affects osteoclast function in vitro and bone mass in vivo. <span><span class="ref-journal">Proc Natl Acad Sci U S A. </span>2009;<span class="ref-vol">106</span>:16511–16516.</span> [<a href="/pmc/articles/PMC2737440/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2737440</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/19805329" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 19805329</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>7.</dt><dd><div class="bk_ref" id="ml193.r7">Barak LS, Ferguson SS, Zhang J, Martenson C, Meyer T, Caron MG. Internal trafficking and surface mobility of a functionally intact beta2-adrenergic receptor-green fluorescent protein conjugate. <span><span class="ref-journal">Mol Pharmacol. </span>1997;<span class="ref-vol">51</span>:177–84.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/9203621" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 9203621</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>8.</dt><dd><div class="bk_ref" id="ml193.r8">Guo J, Williams DJ, Puhl HL 3rd, Ikeda SR. Inhibition of N-type calcium channels by activation of GPR35, an orphan receptor, heterologously expressed in rat sympathetic neurons. <span><span class="ref-journal">J Pharmacol Exp Ther. </span>2008;<span class="ref-vol">324</span>:342–51.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/17940199" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 17940199</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>9.</dt><dd><div class="bk_ref" id="ml193.r9">Johns DG, Behm DJ, Walker DJ, Ao Z, Shapland EM, Daniels DA, Riddick M, Dowell S, Staton PC, Green P, Shabon U, Bao W, Aiyar N, Yue TL, Brown AJ, Morrison AD, Douglas SA. The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects. <span><span class="ref-journal">Br J Pharmacol. </span>2007;<span class="ref-vol">152</span>:825–31.</span> [<a href="/pmc/articles/PMC2190033/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2190033</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/17704827" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 17704827</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>10.</dt><dd><div class="bk_ref" id="ml193.r10">Ryberg E, Larsson N, Sjogren S, Hjorth S, Hermansson NO, Leonova J, Elebring T, Nilsson K, Drmota T, Greasley PJ. The orphan receptor GPR55 is a novel cannabinoid receptor. <span><span class="ref-journal">Br J Pharmacol. </span>2007;<span class="ref-vol">152</span>:1092–101.</span> [<a href="/pmc/articles/PMC2095107/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2095107</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/17876302" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 17876302</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>11.</dt><dd><div class="bk_ref" id="ml193.r11">Kapur A, Zhao P, Sharir H, Bai Y, Caron MG, Barak LS, Abood ME. Atypical responsiveness of the orphan receptor GPR55 to cannabinoid ligands. <span><span class="ref-journal">J Biol Chem. </span>2009 Oct 23;<span class="ref-vol">284</span>(43):29817–27.</span> [<a href="/pmc/articles/PMC2785612/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2785612</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/19723626" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 19723626</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>12.</dt><dd><div class="bk_ref" id="ml193.r12">Taniguchi Y, Tonai-Kachi H, Shinjo K. Zaprinast, a well-known cyclic guanosine monophosphate-specific phosphodiesterase inhibitor, is an agonist for GPR35. <span><span class="ref-journal">FEBS Lett. </span>2006;<span class="ref-vol">580</span>:5003–8.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/16934253" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 16934253</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>13.</dt><dd><div class="bk_ref" id="ml193.r13">Brown AJ. Novel cannabinoid receptors. <span><span class="ref-journal">Br J Pharmacol. </span>2007;<span class="ref-vol">152</span>:567–75.</span> [<a href="/pmc/articles/PMC2190013/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC2190013</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/17906678" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 17906678</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>14.</dt><dd><div class="bk_ref" id="ml193.r14">Barak LS, Ferguson SS, Zhang J, Caron MG. A beta-arrestin/green fluorescent protein biosensor for detecting G protein-coupled receptor activation. <span><span class="ref-journal">J Biol Chem. </span>1997;<span class="ref-vol">272</span>:27497–500.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/9346876" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 9346876</span></a>]</div></dd></dl></dl></div><div id="bk_toc_contnr"></div></div></div><div class="fm-sec"><h2 id="_NBK66153_pubdet_">Publication Details</h2><h3>Author Information and Affiliations</h3><p class="contrib-group"><h4>Authors</h4><span itemprop="author">Susanne Heynen-Genel</span>, <span itemprop="author">Russell Dahl</span>, <span itemprop="author">Shenghua Shi</span>, <span itemprop="author">Loribelle Milan</span>, <span itemprop="author">Santosh Hariharan</span>, <span itemprop="author">Eduard Sergienko</span>, <span itemprop="author">Michael Hedrick</span>, <span itemprop="author">Shakeela Dad</span>, <span itemprop="author">Derek Stonich</span>, <span itemprop="author">Ying Su</span>, <span itemprop="author">Michael Vicchiarelli</span>, <span itemprop="author">Arianna Mangravita-Novo</span>, <span itemprop="author">Layton H Smith</span>, <span itemprop="author">Thomas DY Chung</span>, <span itemprop="author">Haleli Sharir</span>, <span itemprop="author">Marc G Caron</span>, <span itemprop="author">Lawrence S Barak</span>, and <span itemprop="author">Mary E Abood</span>.</p><h3>Publication History</h3><p class="small">Received: <span itemprop="datePublished">October 30, 2010</span>; Last Update: <span itemprop="dateModified">May 26, 2011</span>.</p><h3>Copyright</h3><div><div class="half_rhythm"><a href="/books/about/copyright/">Copyright Notice</a></div></div><h3>Publisher</h3><p>National Center for Biotechnology Information (US), Bethesda (MD)</p><h3>NLM Citation</h3><p>Heynen-Genel S, Dahl R, Shi S, et al. Screening for Selective Ligands for GPR55 - Antagonists. 2010 Oct 30 [Updated 2011 May 26]. In: Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-. <span class="bk_cite_avail"></span></p></div><div class="small-screen-prev"><a href="/books/n/mlprobe/ml194/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M75,30 c-80,60 -80,0 0,60 c-30,-60 -30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Prev</text></svg></a></div><div class="small-screen-next"><a href="/books/n/mlprobe/ml190/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M25,30c80,60 80,0 0,60 c30,-60 30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Next</text></svg></a></div></article><article data-type="fig" id="figobml193fu1"><div id="ml193.fu1" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu1.jpg" alt="ML193." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></span></h3><div class="caption"><p>CID 1261822</p></div></div></article><article data-type="fig" id="figobml193fu2"><div id="ml193.fu2" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu2.jpg" alt="ML192." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></span></h3><div class="caption"><p>CID 1434953</p></div></div></article><article data-type="fig" id="figobml193fu3"><div id="ml193.fu3" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu3.jpg" alt="ML191." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></span></h3><div class="caption"><p>CID 23612552</p></div></div></article><article data-type="table-wrap" id="figobml193tu1"><div id="ml193.tu1" class="table"><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.tu1/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.tu1_lrgtbl__"><table><thead><tr><th id="hd_h_ml193.tu1_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CID<br />[ML]</th><th id="hd_h_ml193.tu1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Target Name</th><th id="hd_h_ml193.tu1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">IC<sub>50</sub>/EC<sub>50</sub> (nM) [SID, AID]</th><th id="hd_h_ml193.tu1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Anti-target Name(s)</th><th id="hd_h_ml193.tu1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">IC<sub>50</sub>/EC<sub>50</sub> (μM) [SID, AID]</th><th id="hd_h_ml193.tu1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Selectivity</th><th id="hd_h_ml193.tu1_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Secondary Assay(s) Name: IC<sub>50</sub>/EC<sub>50</sub> (nM) [SID, AID]</th></tr><tr><th headers="hd_h_ml193.tu1_1_1_1_1 hd_h_ml193.tu1_1_1_1_2 hd_h_ml193.tu1_1_1_1_3 hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_1_7" id="hd_h_ml193.tu1_1_1_2_1" colspan="7" rowspan="1" style="text-align:left;vertical-align:bottom;">
|
|
<span class="hr"></span></th></tr></thead><tbody><tr><td headers="hd_h_ml193.tu1_1_1_1_1 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">1261822<br /><br /><b>[</b><b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b><b>]</b><br /><br /><i>quinoline aryl sulfonamide</i></td><td headers="hd_h_ml193.tu1_1_1_1_2 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">GPR55<br />Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_3 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">221 nM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2820" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2820</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">GPR35 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2809" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2809</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 145X</td><td headers="hd_h_ml193.tu1_1_1_1_7 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">PKC β II<br />Active @ 10μM [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/488947" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID488947</a>]<br /><br />pERK<br />65 nM [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, AID Pending]</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">GPR35 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2815" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2815</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 145X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB1 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2814" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2814</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 145X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB1 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">24.6 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2835" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2835</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">27.1X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB2 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2822" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2822</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 145X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB2 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2836" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2836</a><b>]</b></td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 145X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_1 hd_h_ml193.tu1_1_1_2_1 hd_h_ml193.tu1_1_1_1_2 hd_h_ml193.tu1_1_1_1_3 hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_1_7" colspan="7" rowspan="1" style="text-align:left;vertical-align:top;">
|
|
<span class="hr"></span></td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_1 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">1434953<br /><br /><b>[</b><b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b><b>]</b><br /><br /><i>thienopyrimidine</i></td><td headers="hd_h_ml193.tu1_1_1_1_2 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">GPR55<br />Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_3 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">702 nM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2820" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2820</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">GPR35 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2809" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2809</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 45X</td><td headers="hd_h_ml193.tu1_1_1_1_7 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">PKC β II<br />Active @ 100μM [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/488947" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID488947</a>]<br /><br />pERK<br />827 μM [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, AID Pending]</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">GPR35 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2815" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2815</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 45X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB1 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2814" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2814</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 45X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB1 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2822" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2822</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 45X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB2 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2835" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2835</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 45X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB2 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225747</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2836" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2836</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 45X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_1 hd_h_ml193.tu1_1_1_2_1 hd_h_ml193.tu1_1_1_1_2 hd_h_ml193.tu1_1_1_1_3 hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_1_7" colspan="7" rowspan="1" style="text-align:left;vertical-align:top;">
|
|
<span class="hr"></span></td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_1 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">23612552<br /><br /><b>[</b><b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b><b>]</b><br /><br /><i>pipera-dinyloxadiazolone</i></td><td headers="hd_h_ml193.tu1_1_1_1_2 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">GPR55<br />Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_3 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">1,076 nM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2820" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2820</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">GPR35 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2809" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2809</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 29X</td><td headers="hd_h_ml193.tu1_1_1_1_7 hd_h_ml193.tu1_1_1_2_1" rowspan="6" colspan="1" style="text-align:center;vertical-align:top;">PKC β II<br />Active @ 30μM [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/488947" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID488947</a>]<br /><br />pERK<br />328 nM [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225762</a>, AID Pending]</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">GPR35 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2815" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2815</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 29X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB1 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2814" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2814</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 29X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB1 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">17.7 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2822" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2822</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">16.4X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB2 Agonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM EC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2835" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2835</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 29X</td></tr><tr><td headers="hd_h_ml193.tu1_1_1_1_4 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">CB2 Antagonist</td><td headers="hd_h_ml193.tu1_1_1_1_5 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">>32 μM IC<sub>50</sub> [<a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">SID87225750</a>, <a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2836" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID2836</a>]</td><td headers="hd_h_ml193.tu1_1_1_1_6 hd_h_ml193.tu1_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">> 29X</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml193f1"><div id="ml193.f1" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193f1.jpg" alt="Figure 1. Previously reported GPR55 agonists." /></div><h3><span class="label">Figure 1</span><span class="title">Previously reported GPR55 agonists</span></h3></div></article><article data-type="table-wrap" id="figobml193t1"><div id="ml193.t1" class="table"><h3><span class="label">Table 1</span><span class="title">Summary of Assays and AIDs</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t1/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t1_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">PubChemBioAssay Name</th><th id="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">AIDs</th><th id="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Probe Type</th><th id="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Assay Type</th><th id="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Assay Format</th><th id="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Assay Detection & well format</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Summary of Image-based HTS for Selective Antagonists of GPR55 [Summary]</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2026" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2026</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Antagonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Summary</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">N/A</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">N/A</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Image-based HTS for Selective Antagonists of GPR55 [Confirmatory]</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2013" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2013</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Antagonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Primary</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">SAR analysis of selective Antagonists of GPR55 using an Image-Based Assay [Confirmatory]</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2820" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2820</a><br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485287" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485287</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Antagonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">SAR</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">SAR analysis of Agonists of the GPR35 Receptor using an Image-Based Assay</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2809" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2809</a><br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485286" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485286</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Agonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Selectivity</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">SAR analysis of Antagonists of the GPR35 Receptor using an Image-Based Assay</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2815" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2815</a><br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485283" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485283</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Antagonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Selectivity</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">SAR analysis of agonists of the Cannabinoid Receptor 1 using an Image-Based Assay</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2814" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2814</a><br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485282" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485282</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Agonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Selectivity</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">SAR analysis of antagonists of the Cannabinoid Receptor 1 using an Image-Based Assay</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2822" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2822</a><br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485285" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485285</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Antagonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Selectivity</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">SAR analysis of agonists of the Cannabinoid Receptor 2 using an Image-Based Assay</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2835" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2835</a><br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485277" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485277</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Agonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Selectivity</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr><tr><td headers="hd_h_ml193.t1_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">SAR analysis of antagonists of the Cannabinoid Receptor 2 using an Image-Based Assay</td><td headers="hd_h_ml193.t1_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/2836" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">2836</a><br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/485278" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">485278</a></td><td headers="hd_h_ml193.t1_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Antagonist</td><td headers="hd_h_ml193.t1_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Selectivity</td><td headers="hd_h_ml193.t1_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cell-based</td><td headers="hd_h_ml193.t1_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">HCS – TransFluor & 384</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml193f2"><div id="ml193.f2" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193f2.jpg" alt="Figure 2. GPR55 Example Images of Positive and Negative Controls." /></div><h3><span class="label">Figure 2</span><span class="title">GPR55 Example Images of Positive and Negative Controls</span></h3><div class="caption"><p>Effect of 25 μM agonist LPI (left panel) compared to DMSO control (right panel)</p></div></div></article><article data-type="table-wrap" id="figobml193t2"><div id="ml193.t2" class="table"><h3><span class="label">Table 2</span><span class="title">Reagents used for the uHTS experiments</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t2/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t2_lrgtbl__"><table class="no_top_margin"><thead><tr><th id="hd_h_ml193.t2_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">Reagent</th><th id="hd_h_ml193.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">Vendor</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t2_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">U2OS (Human Osteosarcoma) cell line stably expressing GFP-tagged β-arrestin and over-expressing the GPR55 receptor</td><td headers="hd_h_ml193.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Cells from AP, scaled-up by SBCCG</td></tr><tr><td headers="hd_h_ml193.t2_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">MEM with L-glutamine, Pen-strep, 10% Fetal Bovine Serum and selection antibiotics - 100ug/ml G418 and 50ug/ml Zeocin</td><td headers="hd_h_ml193.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Invitrogen</td></tr><tr><td headers="hd_h_ml193.t2_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">384-well plates, black with clear bottom</td><td headers="hd_h_ml193.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Greiner</td></tr><tr><td headers="hd_h_ml193.t2_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Agonist Lysophosphatidylinositol</td><td headers="hd_h_ml193.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Avanti Polar</td></tr><tr><td headers="hd_h_ml193.t2_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Paraformaldehyde - Fixative</td><td headers="hd_h_ml193.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">ACROS Organics</td></tr><tr><td headers="hd_h_ml193.t2_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">DAPI – Nuclei Stain</td><td headers="hd_h_ml193.t2_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Invitrogen</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml193fu4"><div id="ml193.fu4" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu4.jpg" alt="Critical Path Flowchart for GPR55 Antagonist Project." /></div><h3><span class="title">Critical Path Flowchart for GPR55 Antagonist Project</span></h3></div></article><article data-type="fig" id="figobml193fu5"><div id="ml193.fu5" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu5.jpg" alt="GPR55 Antagonist Compound Flowchart." /></div><h3><span class="title">GPR55 Antagonist Compound Flowchart</span></h3></div></article><article data-type="table-wrap" id="figobml193t3"><div id="ml193.t3" class="table"><h3><span class="label">Table 3</span><span class="title">SAR of GPR55 Antagonist Probe <a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a> (#1): CID1261822</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t3/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t3_lrgtbl__"><table class="no_margin"><thead><tr><th id="hd_h_ml193.t3_1_1_1_1" colspan="3" rowspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu6.jpg" alt="Image ml193fu6.jpg" /></div></th><th id="hd_h_ml193.t3_1_1_1_2" rowspan="2" colspan="1" headers="hd_h_ml193.t3_1_1_1_2" style="text-align:center;vertical-align:middle;">SBCCG MLS#</th><th id="hd_h_ml193.t3_1_1_1_3" rowspan="2" colspan="1" headers="hd_h_ml193.t3_1_1_1_3" style="text-align:center;vertical-align:middle;">PubChem SID</th><th id="hd_h_ml193.t3_1_1_1_4" rowspan="2" colspan="1" headers="hd_h_ml193.t3_1_1_1_4" style="text-align:center;vertical-align:middle;">PubChem CID</th><th id="hd_h_ml193.t3_1_1_1_5" colspan="7" rowspan="1" style="text-align:center;vertical-align:middle;">Potency IC50 antagonists or EC50 agonists (μM) ± S.E.M.</th></tr><tr><th headers="hd_h_ml193.t3_1_1_1_1" id="hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">R<sub>1</sub></th><th headers="hd_h_ml193.t3_1_1_1_1" id="hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">R<sub>2</sub></th><th headers="hd_h_ml193.t3_1_1_1_1" id="hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">R<sub>3</sub></th><th headers="hd_h_ml193.t3_1_1_1_5" id="hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GPR55 Antagonist [n=3]<sup>*</sup></th><th headers="hd_h_ml193.t3_1_1_1_5" id="hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB1 Agonist [n=2]</th><th headers="hd_h_ml193.t3_1_1_1_5" id="hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB1 Antagonist [n=4]<sup>*</sup></th><th headers="hd_h_ml193.t3_1_1_1_5" id="hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB2 Agonist [n=2]</th><th headers="hd_h_ml193.t3_1_1_1_5" id="hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB2 Antagonist [n=2]</th><th headers="hd_h_ml193.t3_1_1_1_5" id="hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GPR35 Agonist [n=2]</th><th headers="hd_h_ml193.t3_1_1_1_5" id="hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GRP35 Antagonist [n=2]</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu7.jpg" alt="Image ml193fu7.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu8.jpg" alt="Image ml193fu8.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0269911</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225762</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1261822</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.221 ± 0.043 [n=4]</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24.6 ± 4.6 [n=2]</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu9.jpg" alt="Image ml193fu9.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu10.jpg" alt="Image ml193fu10.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0266854</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225761" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225761</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1261290</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.457 ± 0.150 [n=2]</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu11.jpg" alt="Image ml193fu11.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu12.jpg" alt="Image ml193fu12.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0098578</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225763" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225763</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1261872</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.709 ± 0.247 [n=2]</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu13.jpg" alt="Image ml193fu13.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu14.jpg" alt="Image ml193fu14.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0257792</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303185" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303185</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2332853</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4.63 ± 0.27</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">18.8 ± 1.6</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu15.jpg" alt="Image ml193fu15.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu16.jpg" alt="Image ml193fu16.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0206672</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303168" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303168</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1582687</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">5.75 ± 1.17</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4.77 ± 0.49</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu17.jpg" alt="Image ml193fu17.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu18.jpg" alt="Image ml193fu18.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0226099</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303183" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303183</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2308203</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.7 ± 3.9</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4.92 ± 0.72</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu19.jpg" alt="Image ml193fu19.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu20.jpg" alt="Image ml193fu20.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0295640</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303186" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303186</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2333197</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">18.2 ± 6.2</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">16.9 ± 0.5</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu21.jpg" alt="Image ml193fu21.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu22.jpg" alt="Image ml193fu22.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437218</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303172" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303172</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2164452</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">29.0 [n=1]</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4.09 ± 1.64</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu23.jpg" alt="Image ml193fu23.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu24.jpg" alt="Image ml193fu24.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0203836</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303171" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303171</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1358622</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">7.70 [n=1]</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10.2 ± 2.4</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu25.jpg" alt="Image ml193fu25.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu26.jpg" alt="Image ml193fu26.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0295687</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303184" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303184</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2330731</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24.5 ± 0.8 [n=3]</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu27.jpg" alt="Image ml193fu27.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu28.jpg" alt="Image ml193fu28.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0101594</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303162" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303162</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1011349</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.14 ± 1.99</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu29.jpg" alt="Image ml193fu29.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu30.jpg" alt="Image ml193fu30.jpg" /></div></td><td headers="hd_h_ml193.t3_1_1_1_1 hd_h_ml193.t3_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t3_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0299650</td><td headers="hd_h_ml193.t3_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303190" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303190</a></td><td headers="hd_h_ml193.t3_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2385808</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.23 ± 0.26</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t3_1_1_1_5 hd_h_ml193.t3_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>*</dt><dd><div id="ml193.tfn1"><p class="no_margin">replicates n=2,3 or 4 are as indicated for columns; the actual replicate number is noted in the cell where different than column default</p></div></dd></dl></dl></div></div></div></article><article data-type="table-wrap" id="figobml193t4"><div id="ml193.t4" class="table"><h3><span class="label">Table 4</span><span class="title">SAR of GPR55 Antagonist Probe <a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a> (#2): CID1434953</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t4/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t4_lrgtbl__"><table class="no_margin"><thead><tr><th id="hd_h_ml193.t4_1_1_1_1" colspan="3" rowspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu31.jpg" alt="Image ml193fu31.jpg" /></div></th><th id="hd_h_ml193.t4_1_1_1_2" rowspan="2" colspan="1" headers="hd_h_ml193.t4_1_1_1_2" style="text-align:center;vertical-align:middle;">SBCCG MLS#</th><th id="hd_h_ml193.t4_1_1_1_3" rowspan="2" colspan="1" headers="hd_h_ml193.t4_1_1_1_3" style="text-align:center;vertical-align:middle;">PubChem SID</th><th id="hd_h_ml193.t4_1_1_1_4" rowspan="2" colspan="1" headers="hd_h_ml193.t4_1_1_1_4" style="text-align:center;vertical-align:middle;">PubChem CID</th><th id="hd_h_ml193.t4_1_1_1_5" colspan="7" rowspan="1" style="text-align:center;vertical-align:middle;">Potency IC50 antagonists or EC50 agonists (μM) ± S.E.M.</th></tr><tr><th headers="hd_h_ml193.t4_1_1_1_1" id="hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">R<sub>1</sub></th><th headers="hd_h_ml193.t4_1_1_1_1" id="hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">R<sub>2</sub></th><th headers="hd_h_ml193.t4_1_1_1_1" id="hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">R<sub>3</sub></th><th headers="hd_h_ml193.t4_1_1_1_5" id="hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GPR55 Antagonist [n=3]<sup>*</sup></th><th headers="hd_h_ml193.t4_1_1_1_5" id="hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB1 Agonist [n=2]</th><th headers="hd_h_ml193.t4_1_1_1_5" id="hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB1 Antagonist [n=4]<sup>*</sup></th><th headers="hd_h_ml193.t4_1_1_1_5" id="hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB2 Agonist [n=2]<sup>*</sup></th><th headers="hd_h_ml193.t4_1_1_1_5" id="hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB2 Antagonist [n=2]<sup>*</sup></th><th headers="hd_h_ml193.t4_1_1_1_5" id="hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GPR35 Agonist [n=2]<sup>*</sup></th><th headers="hd_h_ml193.t4_1_1_1_5" id="hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GRP35 Antagonists [n=2]</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu32.jpg" alt="Image ml193fu32.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0285143</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225742" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225742</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3193014</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.435 ± 0.108 [n=4]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.17 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">28.0 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu33.jpg" alt="Image ml193fu33.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0083944</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225747</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434953</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.702 ± 0.224 [n=2]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu34.jpg" alt="Image ml193fu34.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu35.jpg" alt="Image ml193fu35.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0043335</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225740" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225740</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">655864</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.865 ± 0.366 [n=2]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu36.jpg" alt="Image ml193fu36.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0354783</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303131" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303131</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434956</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4.13 ± 0.91</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.94 ± 0.94</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu37.jpg" alt="Image ml193fu37.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437131</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303126" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303126</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434957</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.24 ± 3.76</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.05 ± 0.43</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">16.8 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu38.jpg" alt="Image ml193fu38.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437135</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303130" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303130</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3193022</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10.6 ± 2.9</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">17.9 ± 1.83</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu39.jpg" alt="Image ml193fu39.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437136</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303132" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303132</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434960</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.1 ± 4.6</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.22 ± 0.56</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.1 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu40.jpg" alt="Image ml193fu40.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437132</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303127" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303127</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1468079</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.0 ± 7.5</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">6.66 ± 2.47</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">15.2 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu41.jpg" alt="Image ml193fu41.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437134</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303129" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303129</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3193015</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">21.5 ± 4.6</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.68 ± 0.24</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24.1 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.18 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu42.jpg" alt="Image ml193fu42.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437137</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303133" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303133</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434989</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">21.4 ± 6.95 [n=2]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.36 ± 0.26</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">24.2 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu43.jpg" alt="Image ml193fu43.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437226</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303189" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303189</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">17446092</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22.7 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">14.7 ± 3.5 [n=3]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu44.jpg" alt="Image ml193fu44.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437133</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303128" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303128</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434959</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.66 ± 0.20</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11.0 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu45.jpg" alt="Image ml193fu45.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437138</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303134" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303134</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3197465</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">19.6 ± 5.1 [n=2]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">26.8 [n=1]</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu46.jpg" alt="Image ml193fu46.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437140</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303136" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303136</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">46864267</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">13.3 ± 3.4</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Me</td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu47.jpg" alt="Image ml193fu47.jpg" /></div></td><td headers="hd_h_ml193.t4_1_1_1_1 hd_h_ml193.t4_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">H</td><td headers="hd_h_ml193.t4_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437224</td><td headers="hd_h_ml193.t4_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303187" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303187</a></td><td headers="hd_h_ml193.t4_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4877555</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_9" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t4_1_1_1_5 hd_h_ml193.t4_1_1_2_10" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>*</dt><dd><div id="ml193.tfn2"><p class="no_margin">replicates n=2,3 or 4 are as indicated for columns; the actual replicate number is noted in the cell where different than column default</p></div></dd></dl></dl></div></div></div></article><article data-type="table-wrap" id="figobml193t5"><div id="ml193.t5" class="table"><h3><span class="label">Table 5</span><span class="title">SAR of GPR55 Antagonist Probe <a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a> (#3): CID23612552</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t5/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t5_lrgtbl__"><table class="no_margin"><thead><tr><th id="hd_h_ml193.t5_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu48.jpg" alt="Image ml193fu48.jpg" /></div></th><th id="hd_h_ml193.t5_1_1_1_2" rowspan="2" colspan="1" headers="hd_h_ml193.t5_1_1_1_2" style="text-align:center;vertical-align:middle;">SBCCG MLS#</th><th id="hd_h_ml193.t5_1_1_1_3" rowspan="2" colspan="1" headers="hd_h_ml193.t5_1_1_1_3" style="text-align:center;vertical-align:middle;">PubChem SID</th><th id="hd_h_ml193.t5_1_1_1_4" rowspan="2" colspan="1" headers="hd_h_ml193.t5_1_1_1_4" style="text-align:center;vertical-align:middle;">PubChem CID</th><th id="hd_h_ml193.t5_1_1_1_5" colspan="7" rowspan="1" style="text-align:center;vertical-align:middle;">Potency IC50 antagonists or EC50 agonists (μM) ± S.E.M.</th></tr><tr><th headers="hd_h_ml193.t5_1_1_1_1" id="hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">R</th><th headers="hd_h_ml193.t5_1_1_1_5" id="hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GPR55 Antagonist [n=3]<sup>*</sup></th><th headers="hd_h_ml193.t5_1_1_1_5" id="hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB1 Agonist [n=2]</th><th headers="hd_h_ml193.t5_1_1_1_5" id="hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB1 Antagonist [n=4]<sup>*</sup></th><th headers="hd_h_ml193.t5_1_1_1_5" id="hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB2 Agonist [n=2]</th><th headers="hd_h_ml193.t5_1_1_1_5" id="hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CB2 Antagonist [n=2]<sup>*</sup></th><th headers="hd_h_ml193.t5_1_1_1_5" id="hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GPR35 Agonist [n=2]</th><th headers="hd_h_ml193.t5_1_1_1_5" id="hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">GRP35 Antagonists [n=2]</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu49.jpg" alt="Image ml193fu49.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0359058</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225751" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225751</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612567</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.760 ± 0.133 [n=2]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.62 ± 1.58 [n=2]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu50.jpg" alt="Image ml193fu50.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0368126</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225750</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612552</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.08 ± 0.10 [n=2]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">17.7 [n=1]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu51.jpg" alt="Image ml193fu51.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0367705</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303152" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303152</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612550</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.55 ± 2.62</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">7.11 [n=1]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu52.jpg" alt="Image ml193fu52.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437151</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303149" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303149</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612543</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2.70 ± 0.98</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.98 ± 0.90</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">14.0 ± 1.0</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu53.jpg" alt="Image ml193fu53.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437149</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303147" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303147</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612541</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">7.62 ± 1.38 [n=2]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3.31 ± 0.58</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">22.9 [n=1]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu54.jpg" alt="Image ml193fu54.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0359128</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303154" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303154</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612554</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">17.4 ± 9.89 [n=2]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">8.64 ± 1.39</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu55.jpg" alt="Image ml193fu55.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437153</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303151" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303151</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612548</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">15.8 ± 1.9</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu56.jpg" alt="Image ml193fu56.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437150</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303148" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303148</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612542</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">17.9 ± 3.4 [n=3]</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu57.jpg" alt="Image ml193fu57.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437152</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303150" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303150</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612544</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11.7 ± 1.5</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr><tr><td headers="hd_h_ml193.t5_1_1_1_1 hd_h_ml193.t5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">
|
|
<div class="graphic"><img src="/books/NBK66153/bin/ml193fu58.jpg" alt="Image ml193fu58.jpg" /></div></td><td headers="hd_h_ml193.t5_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS-0437154</td><td headers="hd_h_ml193.t5_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303153" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303153</a></td><td headers="hd_h_ml193.t5_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612553</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Avg 7.68 ± 0.78</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td><td headers="hd_h_ml193.t5_1_1_1_5 hd_h_ml193.t5_1_1_2_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>32</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>*</dt><dd><div id="ml193.tfn3"><p class="no_margin">replicates n=2,3 or 4 are as indicated for columns; the actual replicate number is noted in the cell where different than column default</p></div></dd></dl></dl></div></div></div></article><article data-type="fig" id="figobml193fu6"><div id="ml193.fu6" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu59.jpg" alt="ML 193." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 193</a></span></h3><div class="caption"><p>CID 1261822</p></div></div></article><article data-type="fig" id="figobml193fu7"><div id="ml193.fu7" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu60.jpg" alt="ML 192." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 192</a></span></h3><div class="caption"><p>CID 1434953</p></div></div></article><article data-type="fig" id="figobml193fu8"><div id="ml193.fu8" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu61.jpg" alt="ML 191." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML 191</a></span></h3><div class="caption"><p>CID 23612552</p></div></div></article><article data-type="fig" id="figobml193fu9"><div id="ml193.fu9" class="figure"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu62.jpg" alt="Image ml193fu62" /></div></div></article><article data-type="fig" id="figobml193fu10"><div id="ml193.fu10" class="figure"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu63.jpg" alt="Image ml193fu63" /></div></div></article><article data-type="fig" id="figobml193fu11"><div id="ml193.fu11" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu64.jpg" alt="1H NMR." /></div><h3><span class="title"><sup>1</sup>H NMR</span></h3></div></article><article data-type="fig" id="figobml193fu12"><div id="ml193.fu12" class="figure"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu65.jpg" alt="Image ml193fu65" /></div></div></article><article data-type="fig" id="figobml193fu13"><div id="ml193.fu13" class="figure"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu66.jpg" alt="Image ml193fu66" /></div></div></article><article data-type="fig" id="figobml193fu14"><div id="ml193.fu14" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu67.jpg" alt="1H NMR." /></div><h3><span class="title"><sup>1</sup>H NMR</span></h3></div></article><article data-type="fig" id="figobml193fu15"><div id="ml193.fu15" class="figure"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu68.jpg" alt="Image ml193fu68" /></div></div></article><article data-type="fig" id="figobml193fu16"><div id="ml193.fu16" class="figure"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu69.jpg" alt="Image ml193fu69" /></div></div></article><article data-type="fig" id="figobml193fu17"><div id="ml193.fu17" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu70.jpg" alt="1H NMR." /></div><h3><span class="title"><sup>1</sup>H NMR</span></h3></div></article><article data-type="table-wrap" id="figobml193t6"><div id="ml193.t6" class="table"><h3><span class="label">Table 6</span><span class="title">Probe Submission for GPR55 Antagonist Probes</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t6/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t6_lrgtbl__"><table class="no_margin"><thead><tr><th id="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Probe/Analog</th><th id="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">DPI MLS#<sup>*</sup></th><th id="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">BCCG MLS-#</th><th id="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID</th><th id="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">SID</th><th id="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Source (vendor or BCCG syn)</th><th id="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Amt (mg)</th><th id="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Date<sup>*</sup> Submitted</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t6_1_1_1_1 hd_h_ml193.t6_1_1_1_2 hd_h_ml193.t6_1_1_1_3 hd_h_ml193.t6_1_1_1_4 hd_h_ml193.t6_1_1_1_5 hd_h_ml193.t6_1_1_1_6 hd_h_ml193.t6_1_1_1_7 hd_h_ml193.t6_1_1_1_8" colspan="8" rowspan="1" style="text-align:center;vertical-align:middle;"><b>Probe 1 –</b>
|
|
<b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b><b>: CID1261822</b></td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Probe 1<br /><b>[</b>
|
|
<b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b><b>]</b></td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183099</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0269911</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1261822</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225762</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemBridge</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">25</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11/11/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 1</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183104</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0269911</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1261290</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225761" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225761</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemBridge</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11/11/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 2</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183100</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0098578</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1261872</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225763" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225763</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemBridge</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11/11/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 3</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183103</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0257792</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2332853</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303185" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303185</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11/11/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 4</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183100</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0206672</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1582687</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303168" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303168</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">InterBioScreen</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11/11/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 5</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183102</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0226099</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">2308203</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303183" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303183</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Enamine</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11/11/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1 hd_h_ml193.t6_1_1_1_2 hd_h_ml193.t6_1_1_1_3 hd_h_ml193.t6_1_1_1_4 hd_h_ml193.t6_1_1_1_5 hd_h_ml193.t6_1_1_1_6 hd_h_ml193.t6_1_1_1_7 hd_h_ml193.t6_1_1_1_8" colspan="8" rowspan="1" style="text-align:center;vertical-align:middle;"><b>Probe 2 –</b>
|
|
<b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b><b>: CID1434953</b></td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Probe 2<br /><b>[</b><b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b><b>]</b></td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183105</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0083944</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434953</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225747</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Asinex</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">25</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 1</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183106</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0285143</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3193014</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225742" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225742</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Asinex</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 2</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183110</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0437136</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434960</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303132" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303132</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Asinex</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 3</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183107</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0354783</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434956</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303131" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303131</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Asinex</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 4</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183108</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0437131</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1434957</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303126" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303126</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Asinex</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 5</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183109</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0437135</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">3193022</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303130" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303130</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Asinex</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1 hd_h_ml193.t6_1_1_1_2 hd_h_ml193.t6_1_1_1_3 hd_h_ml193.t6_1_1_1_4 hd_h_ml193.t6_1_1_1_5 hd_h_ml193.t6_1_1_1_6 hd_h_ml193.t6_1_1_1_7 hd_h_ml193.t6_1_1_1_8" colspan="8" rowspan="1" style="text-align:center;vertical-align:middle;"><b>Probe 3-</b>
|
|
<b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b><b>: CID23612552</b></td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Probe 3<br /><b>[</b><b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b><b>]</b></td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183111</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0368126</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612552</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225750</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">25</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 1</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183112</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0359058</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612567</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225751" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225751</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 2</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183114</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0367705</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612550</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303152" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303152</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 3</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183115</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0437151</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612543</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303149" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303149</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 4</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183113</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0359128</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612554</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303154" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303154</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr><tr><td headers="hd_h_ml193.t6_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">Analog 5</td><td headers="hd_h_ml193.t6_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">MLS003183116</td><td headers="hd_h_ml193.t6_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0437153</td><td headers="hd_h_ml193.t6_1_1_1_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">23612548</td><td headers="hd_h_ml193.t6_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/99303151" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">99303151</a></td><td headers="hd_h_ml193.t6_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">ChemDiv</td><td headers="hd_h_ml193.t6_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">20</td><td headers="hd_h_ml193.t6_1_1_1_8" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10/20/10</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>*</dt><dd><div id="ml193.tfn4"><p class="no_margin">Compounds ordered on 10/20/10, but inordinate wait to receive, also experienced submission delays on vial requests</p></div></dd></dl></dl></div></div></div></article><article data-type="fig" id="figobml193fu18"><div id="ml193.fu18" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu71.jpg" alt="Dose response curves for the 3 probes for the GPR55 antagonist assay and all selectivity assays." /></div><h3><span class="title">Dose response curves for the 3 probes for the GPR55 antagonist assay and all selectivity assays</span></h3><div class="caption"><p>% Efficacy is reported representing either % Inhibition (antagonist assays) or % Activation (agonist assays) as compared to control compounds specific to each assay (refer to PubChem AIDs for details)</p></div></div></article><article data-type="fig" id="figobml193f3"><div id="ml193.f3" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193f3.jpg" alt="1a" /></div><h3><span class="label">1a</span></h3></div></article><article data-type="fig" id="figobml193f4"><div id="ml193.f4" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193f4.jpg" alt="1b" /></div><h3><span class="label">1b</span></h3></div></article><article data-type="fig" id="figobml193f5"><div id="ml193.f5" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193f5.jpg" alt="1c" /></div><h3><span class="label">1c</span></h3></div></article><article data-type="table-wrap" id="figobml193t7"><div id="ml193.t7" class="table"><h3><span class="label">Table 7</span><span class="title">Summary of <i>in vitro</i> ADMET/PK Properties of GP55 Agonist Probe(s)</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t7/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t7_lrgtbl__"><table class="no_margin"><thead><tr><th id="hd_h_ml193.t7_1_1_1_1" rowspan="2" colspan="1" headers="hd_h_ml193.t7_1_1_1_1" style="text-align:center;vertical-align:top;">Probe CID<br />Probe ML#<br />BCCG MLS-#</th><th id="hd_h_ml193.t7_1_1_1_2" rowspan="2" colspan="1" headers="hd_h_ml193.t7_1_1_1_2" style="text-align:center;vertical-align:top;">Aqueous Solubility (μg/mL)<sup>a</sup> (@ pH)<br /><br />(1×PBS 7.4)</th><th id="hd_h_ml193.t7_1_1_1_3" rowspan="2" colspan="1" headers="hd_h_ml193.t7_1_1_1_3" style="text-align:center;vertical-align:top;">PAMPA Pe (×10<sup>−6</sup> cm/s)<sup>b</sup> (@ pH)</th><th id="hd_h_ml193.t7_1_1_1_4" colspan="2" rowspan="1" style="text-align:center;vertical-align:top;">Plasma Protein Binding (% Bound)</th><th id="hd_h_ml193.t7_1_1_1_5" rowspan="2" colspan="1" headers="hd_h_ml193.t7_1_1_1_5" style="text-align:center;vertical-align:top;">Plasma Stability<sup>c</sup><br />Human/Mouse 1× PBS, pH 7.4</th><th id="hd_h_ml193.t7_1_1_1_6" rowspan="2" colspan="1" headers="hd_h_ml193.t7_1_1_1_6" style="text-align:center;vertical-align:top;">Hepatic Microsome Stability<sup>d</sup><br />Human/Mouse<br /><br />NADPH minus</th><th id="hd_h_ml193.t7_1_1_1_7" rowspan="2" colspan="1" headers="hd_h_ml193.t7_1_1_1_7" style="text-align:center;vertical-align:top;">Hepatic Toxicity<sup>e</sup><br />LC50 (μM)</th></tr><tr><th headers="hd_h_ml193.t7_1_1_1_4" id="hd_h_ml193.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Human<br />1μM/10μM</th><th headers="hd_h_ml193.t7_1_1_1_4" id="hd_h_ml193.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">Mouse<br />1μM/10μM</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t7_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID1261822<br /><b><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></b><br />MLS-0269911</td><td headers="hd_h_ml193.t7_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1.8 (5.0)<br />4.3 (6.2)<br />32.8 (7.4)<br />14.8 (PBS)</td><td headers="hd_h_ml193.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">9.5 (5.0)<br />124(6.2)<br />135(7.4)</td><td headers="hd_h_ml193.t7_1_1_1_4 hd_h_ml193.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">99.88/ 99.92</td><td headers="hd_h_ml193.t7_1_1_1_4 hd_h_ml193.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">99.78/ 99.62</td><td headers="hd_h_ml193.t7_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">89.54/90.13<br />3.44/2.41<br />11.77</td><td headers="hd_h_ml193.t7_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">62.11/51.81<br />58.12/49.89</td><td headers="hd_h_ml193.t7_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">26.9</td></tr><tr><td headers="hd_h_ml193.t7_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID1434953<br /><b><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></b><br />MLS-0083944</td><td headers="hd_h_ml193.t7_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">11.1 (5.0)<br />14.2 (6.2)<br />16.9 (7.4)<br />8.3 (PBS)</td><td headers="hd_h_ml193.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1347 (5.0)<br />1230 (6.2)<br />1215 (7.4)</td><td headers="hd_h_ml193.t7_1_1_1_4 hd_h_ml193.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">99.53/ 99.51</td><td headers="hd_h_ml193.t7_1_1_1_4 hd_h_ml193.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">98.65/ 98.44</td><td headers="hd_h_ml193.t7_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">96.85/98.08<br />62.76/58.26<br />45.57</td><td headers="hd_h_ml193.t7_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.02/0.06<br />42.54/7.17</td><td headers="hd_h_ml193.t7_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>50</td></tr><tr><td headers="hd_h_ml193.t7_1_1_1_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">CID23612552<br /><b><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></b><br />MLS-0368126</td><td headers="hd_h_ml193.t7_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">4.5 (5.0)<br />5.0 (6.2)<br />6.4 (7.4)<br />1.0 (PBS)</td><td headers="hd_h_ml193.t7_1_1_1_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">1910 (5.0)<br />2113 (6.2)<br />2349 (7.4)</td><td headers="hd_h_ml193.t7_1_1_1_4 hd_h_ml193.t7_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">99.50/ 99.84</td><td headers="hd_h_ml193.t7_1_1_1_4 hd_h_ml193.t7_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">98.70/ 98.41</td><td headers="hd_h_ml193.t7_1_1_1_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">100/100<br />42.43/51.15<br />24.75</td><td headers="hd_h_ml193.t7_1_1_1_6" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0.99/0.03</td><td headers="hd_h_ml193.t7_1_1_1_7" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">>50</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>a</dt><dd><div id="ml193.tfn5"><p class="no_margin">in aqueous buffer (phosphate-free), pH’s 5.0/6.2/7.4 or 1×PBS pH7.4</p></div></dd></dl><dl class="bkr_refwrap"><dt>b</dt><dd><div id="ml193.tfn6"><p class="no_margin">in aqueous buffer (phosphate-free); Donor compartment pH’s 5.0/6.2/7.4; Acceptor compartment pH 7.4</p></div></dd></dl><dl class="bkr_refwrap"><dt>c</dt><dd><div id="ml193.tfn7"><p class="no_margin">% remaining at 3 hr; in Plasma:1× PBS, pH 7.4, 1:1 @ 37 °C; % remaining at 32 hrs in 1× PBS</p></div></dd></dl><dl class="bkr_refwrap"><dt>d</dt><dd><div id="ml193.tfn8"><p class="no_margin">% remaining at 1 hr</p></div></dd></dl><dl class="bkr_refwrap"><dt>e</dt><dd><div id="ml193.tfn9"><p class="no_margin">towards Fa2N-4 immortalized human hepatocytes</p></div></dd></dl><dl class="bkr_refwrap"><dt>*</dt><dd><div id="ml193.tfn10"><p class="no_margin">Negative value obtained (less compound in the plasma donor chamber); indicative of significant plasma instability</p></div></dd></dl></dl></div></div></div></article><article data-type="fig" id="figobml193fu19"><div id="ml193.fu19" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu72.jpg" alt="ML193 Stability in 1:1 PBS/ACN." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a> Stability in 1:1 PBS/ACN</span></h3></div></article><article data-type="table-wrap" id="figobml193tu2"><div id="ml193.tu2" class="table"><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.tu2/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.tu2_lrgtbl__"><table><thead><tr><th id="hd_h_ml193.tu2_1_1_1_1" colspan="2" rowspan="1" style="text-align:center;vertical-align:bottom;"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML193</a></th></tr><tr><th headers="hd_h_ml193.tu2_1_1_1_1" id="hd_h_ml193.tu2_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">MLS-0269911</th><th headers="hd_h_ml193.tu2_1_1_1_1" id="hd_h_ml193.tu2_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">CID1261822</th></tr><tr><th headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1" id="hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:bottom;"><i>Time (hr)</i></th><th headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2" id="hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:bottom;"><i>Remaining</i></th></tr></thead><tbody><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">0</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.0%</td></tr><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">1</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">98.0%</td></tr><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">2</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">98.6%</td></tr><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">3</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">98.4%</td></tr><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">21</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">98.5%</td></tr><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">24</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">99.4%</td></tr><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">45</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">99.2%</td></tr><tr><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_1 hd_h_ml193.tu2_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">48</td><td headers="hd_h_ml193.tu2_1_1_1_1 hd_h_ml193.tu2_1_1_2_2 hd_h_ml193.tu2_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">98.2%</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml193fu20"><div id="ml193.fu20" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu73.jpg" alt="ML192 Stability in 1:1 PBS/ACN." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a> Stability in 1:1 PBS/ACN</span></h3></div></article><article data-type="table-wrap" id="figobml193tu3"><div id="ml193.tu3" class="table"><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.tu3/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.tu3_lrgtbl__"><table><thead><tr><th id="hd_h_ml193.tu3_1_1_1_1" colspan="2" rowspan="1" style="text-align:center;vertical-align:bottom;"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML192</a></th></tr><tr><th headers="hd_h_ml193.tu3_1_1_1_1" id="hd_h_ml193.tu3_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">MLS-0083944</th><th headers="hd_h_ml193.tu3_1_1_1_1" id="hd_h_ml193.tu3_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">CID1434953</th></tr><tr><th headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_1" id="hd_h_ml193.tu3_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:bottom;"><i>Time (hr)</i></th><th headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_2" id="hd_h_ml193.tu3_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:bottom;"><i>Remaining</i></th></tr></thead><tbody><tr><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_1 hd_h_ml193.tu3_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">0</td><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_2 hd_h_ml193.tu3_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.0%</td></tr><tr><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_1 hd_h_ml193.tu3_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">1</td><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_2 hd_h_ml193.tu3_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">99.2%</td></tr><tr><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_1 hd_h_ml193.tu3_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">2</td><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_2 hd_h_ml193.tu3_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">99.2%</td></tr><tr><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_1 hd_h_ml193.tu3_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">24</td><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_2 hd_h_ml193.tu3_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">98.2%</td></tr><tr><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_1 hd_h_ml193.tu3_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">40</td><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_2 hd_h_ml193.tu3_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">97.5%</td></tr><tr><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_1 hd_h_ml193.tu3_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">48</td><td headers="hd_h_ml193.tu3_1_1_1_1 hd_h_ml193.tu3_1_1_2_2 hd_h_ml193.tu3_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">97.1%</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml193fu21"><div id="ml193.fu21" class="figure bk_fig"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu74.jpg" alt="ML191 Stability in 1:1 PBS/ACN." /></div><h3><span class="title"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a> Stability in 1:1 PBS/ACN</span></h3></div></article><article data-type="table-wrap" id="figobml193tu4"><div id="ml193.tu4" class="table"><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.tu4/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.tu4_lrgtbl__"><table><thead><tr><th id="hd_h_ml193.tu4_1_1_1_1" colspan="2" rowspan="1" style="text-align:center;vertical-align:bottom;"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">ML191</a></th></tr><tr><th headers="hd_h_ml193.tu4_1_1_1_1" id="hd_h_ml193.tu4_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">MLS-0368126</th><th headers="hd_h_ml193.tu4_1_1_1_1" id="hd_h_ml193.tu4_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">CID23612552</th></tr><tr><th headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1" id="hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:bottom;"><i>Time (hr)</i></th><th headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2" id="hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:bottom;"><i>Remaining</i></th></tr></thead><tbody><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">0</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.0%</td></tr><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">1</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.0%</td></tr><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">2</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.2%</td></tr><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">3</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.2%</td></tr><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">21</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.3%</td></tr><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">24</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.0%</td></tr><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">45</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.4%</td></tr><tr><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_1 hd_h_ml193.tu4_1_1_3_1" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">48</td><td headers="hd_h_ml193.tu4_1_1_1_1 hd_h_ml193.tu4_1_1_2_2 hd_h_ml193.tu4_1_1_3_2" rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">100.0%</td></tr></tbody></table></div></div></article><article data-type="fig" id="figobml193fu22"><div id="ml193.fu22" class="figure"><div class="graphic"><img data-src="/books/NBK66153/bin/ml193fu75.jpg" alt="Image ml193fu75" /></div></div></article><article data-type="table-wrap" id="figobml193tu5"><div id="ml193.tu5" class="table"><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.tu5/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.tu5_lrgtbl__"><table><thead><tr><th id="hd_h_ml193.tu5_1_1_1_1" rowspan="2" colspan="1" headers="hd_h_ml193.tu5_1_1_1_1" style="text-align:left;vertical-align:middle;">Calculated Property</th><th id="hd_h_ml193.tu5_1_1_1_2" colspan="3" rowspan="1" style="text-align:center;vertical-align:bottom;">Parameter Value</th></tr><tr><th headers="hd_h_ml193.tu5_1_1_1_2" id="hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">CID1261822</th><th headers="hd_h_ml193.tu5_1_1_1_2" id="hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">CID1434953</th><th headers="hd_h_ml193.tu5_1_1_1_2" id="hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:bottom;">CID23612552</th></tr></thead><tbody><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Molecular Weight [g/mol]</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">527.5942</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">382.47928</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">403.4736</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Molecular Formula</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">C<sub>28</sub>H<sub>25</sub>N<sub>5</sub>O<sub>4</sub>S</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">C<sub>20</sub>H<sub>22</sub>N<sub>4</sub>O<sub>2</sub>S</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">C<sub>24</sub>H<sub>25</sub>N<sub>3</sub>O<sub>3</sub></td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">XLogP3-AA</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4.5</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">H-Bond Donor</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">H-Bond Acceptor</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">8</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">5</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Rotatable Bond Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">6</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">2</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">4</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Tautomer Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">33</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"></td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"></td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Exact Mass</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">527.162725</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">382.146347</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">403.189592</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">MonoIsotopic Mass</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">527.162725</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">382.146347</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">403.189592</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Topological Polar Surface Area</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">136</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">90.7</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">62.2</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Heavy Atom Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">38</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">27</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">30</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Formal Charge</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Complexity</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">921</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">556</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">695</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Isotope Atom Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Defined Atom StereoCenter Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Undefined Atom StereoCenter Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Defined Bond StereoCenter Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Undefined Bond StereoCenter Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0</td></tr><tr><td headers="hd_h_ml193.tu5_1_1_1_1" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Covalently-Bonded Unit Count</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td><td headers="hd_h_ml193.tu5_1_1_1_2 hd_h_ml193.tu5_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1</td></tr></tbody></table></div></div></article><article data-type="table-wrap" id="figobml193t8"><div id="ml193.t8" class="table"><h3><span class="label">Table 9</span><span class="title">Downstream assays by Assay Provider</span></h3><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK66153/table/ml193.t8/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__ml193.t8_lrgtbl__"><table class="no_margin"><thead><tr><th id="hd_h_ml193.t8_1_1_1_1" colspan="4" rowspan="1" style="text-align:center;vertical-align:middle;">Compound IDs</th><th id="hd_h_ml193.t8_1_1_1_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">pERK</th><th id="hd_h_ml193.t8_1_1_1_3" colspan="4" rowspan="1" style="text-align:center;vertical-align:middle;">PKCβII Translocation<sup>*</sup></th></tr><tr><th headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_1" id="hd_h_ml193.t8_1_1_2_1" rowspan="2" colspan="1" style="text-align:center;vertical-align:middle;">[ML probe]</th><th headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_2" id="hd_h_ml193.t8_1_1_2_2" rowspan="2" colspan="1" style="text-align:center;vertical-align:middle;">PubChem CID</th><th headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_3" id="hd_h_ml193.t8_1_1_2_3" rowspan="2" colspan="1" style="text-align:center;vertical-align:middle;">PubChem SID</th><th headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_4" id="hd_h_ml193.t8_1_1_2_4" rowspan="2" colspan="1" style="text-align:center;vertical-align:middle;">SBCCG MLS-#</th><th headers="hd_h_ml193.t8_1_1_1_2" id="hd_h_ml193.t8_1_1_2_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">EC50<br /><i>Prelim. Data</i></th><th headers="hd_h_ml193.t8_1_1_1_3" id="hd_h_ml193.t8_1_1_2_6" colspan="4" rowspan="1" style="text-align:center;vertical-align:middle;">Membrane Recruitment/Blebs<br /><a href="https://pubchem.ncbi.nlm.nih.gov/bioassay/488947" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">AID 488947</a></th></tr><tr><th headers="hd_h_ml193.t8_1_1_1_2 hd_h_ml193.t8_1_1_2_5" id="hd_h_ml193.t8_1_1_3_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">μM</th><th headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6" id="hd_h_ml193.t8_1_1_3_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">100μM</th><th headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6" id="hd_h_ml193.t8_1_1_3_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">30μM</th><th headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6" id="hd_h_ml193.t8_1_1_3_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">10μM</th><th headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6" id="hd_h_ml193.t8_1_1_3_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:middle;">0μM</th></tr></thead><tbody><tr><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="/pcsubstance/?term=ML193[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">193</a></td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1261822</td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225762" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225762</a></td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0269911</td><td headers="hd_h_ml193.t8_1_1_1_2 hd_h_ml193.t8_1_1_2_5 hd_h_ml193.t8_1_1_3_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.188</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">+</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">++++</td></tr><tr><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="/pcsubstance/?term=ML192[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">192</a></td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">1434953</td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225747" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225747</a></td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0083944</td><td headers="hd_h_ml193.t8_1_1_1_2 hd_h_ml193.t8_1_1_2_5 hd_h_ml193.t8_1_1_3_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.878</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">+</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">+++</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">++++</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">++++</td></tr><tr><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="/pcsubstance/?term=ML191[synonym]" ref="pagearea=body&targetsite=entrez&targetcat=term&targettype=pubchem">191</a></td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">23612552</td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;"><a href="https://pubchem.ncbi.nlm.nih.gov/substance/87225750" ref="pagearea=body&targetsite=entrez&targetcat=link&targettype=pubchem">87225750</a></td><td headers="hd_h_ml193.t8_1_1_1_1 hd_h_ml193.t8_1_1_2_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0368126</td><td headers="hd_h_ml193.t8_1_1_1_2 hd_h_ml193.t8_1_1_2_5 hd_h_ml193.t8_1_1_3_1" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">0.143</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_2" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">−</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_3" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">+</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_4" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">++</td><td headers="hd_h_ml193.t8_1_1_1_3 hd_h_ml193.t8_1_1_2_6 hd_h_ml193.t8_1_1_3_5" rowspan="1" colspan="1" style="text-align:center;vertical-align:top;">++++</td></tr></tbody></table></div><div class="tblwrap-foot"><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt>*</dt><dd><div id="ml193.tfn11"><p class="no_top_margin">Manual Image Analysis Result Definition: ‘−‘ means no GFP membrane recruitment or blebs formation</p><p>‘+’ means ~1% GFP membrane recruitment or blebs formation</p><p>‘++’ means ~10% GFP membrane recruitment or blebs formation</p><p>‘+++’ means ~30% GFP membrane recruitment or blebs formation</p><p>‘++++’ means ~60% GFP membrane recruitment or blebs formation</p></div></dd></dl></dl></div></div></div></article></div><div id="jr-scripts"><script src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/libs.min.js"> </script><script src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/jr.min.js"> </script></div></div>
|
|
|
|
|
|
|
|
|
|
<!-- Book content -->
|
|
|
|
<script type="text/javascript" src="/portal/portal3rc.fcgi/rlib/js/InstrumentNCBIBaseJS/InstrumentPageStarterJS.js"> </script>
|
|
|
|
|
|
<!-- CE8B5AF87C7FFCB1_0191SID /projects/books/PBooks@9.11 portal104 v4.1.r689238 Tue, Oct 22 2024 16:10:51 -->
|
|
<span id="portal-csrf-token" style="display:none" data-token="CE8B5AF87C7FFCB1_0191SID"></span>
|
|
|
|
<script type="text/javascript" src="//static.pubmed.gov/portal/portal3rc.fcgi/4216699/js/3968615.js" snapshot="books"></script></body>
|
|
</html>
|