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<script type="text/javascript" src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/jr.boots.min.js"> </script><title>64Cu-DOTA-[Pro1,Tyr4]-Bombesin[1-14] - Molecular Imaging and Contrast Agent Database (MICAD) - NCBI Bookshelf</title>
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<meta name="citation_inbook_title" content="Molecular Imaging and Contrast Agent Database (MICAD) [Internet]">
<meta name="citation_title" content="64Cu-DOTA-[Pro1,Tyr4]-Bombesin[1-14]">
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<meta name="citation_date" content="2008/01/08">
<meta name="citation_author" content="Kenneth T. Cheng">
<meta name="citation_author" content="Jason S. Lewis">
<meta name="citation_author" content="Gr&aacute;inne B. Biddlecombe">
<meta name="citation_pmid" content="20641835">
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<meta name="DC.Contributor" content="Kenneth T. Cheng">
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<meta name="DC.Contributor" content="Gr&aacute;inne B. Biddlecombe">
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<meta name="description" content="64Cu-DOTA-[Pro1,Tyr4]-Bombesin[1-14] (64Cu-MP2346) is a peptide analog of human gastrin-releasing peptide (GRP) conjugated with 64Cu, and it was developed for positron emission tomography (PET) imaging of tumors with overexpressed GRP receptors (GRP-R) (1). 64Cu is a positron emitter with a 19.3% abundance and a physical half-life (t&frac12;) of 12.7 h.">
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['h2'],smoothScroll: false" itemprop="text"><div class="iconblock whole_rhythm clearfix ten_col table-wrap" id="figMP2346Cu64T1"><a href="/books/NBK23640/table/MP2346Cu64.T1/?report=objectonly" target="object" title="Table" class="img_link icnblk_img" rid-ob="figobMP2346Cu64T1"><img class="small-thumb" src="/corehtml/pmc/css/bookshelf/2.26/img/table-icon.gif" alt="Table Icon" /></a><div class="icnblk_cntnt"><h4 id="MP2346Cu64.T1"><a href="/books/NBK23640/table/MP2346Cu64.T1/?report=objectonly" target="object" rid-ob="figobMP2346Cu64T1">Table</a></h4><p class="float-caption no_bottom_margin">
<i>In vitro</i>
Rodents
</p></div></div><div id="MP2346Cu64.Background"><h2 id="_MP2346Cu64_Background_">Background</h2><p>[<a href="/sites/entrez?Db=pubmed&#x00026;Cmd=DetailsSearch&#x00026;Term=(86Y-Bombesin)+OR+(gastrin-releasing+peptide+probe)" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubMed</a>]</p><p><sup>64</sup>Cu-DOTA-[Pro<sup>1</sup>,Tyr<sup>4</sup>]-Bombesin[1-14] (<sup>64</sup>Cu-MP2346) is a peptide analog of human gastrin-releasing peptide (GRP) conjugated with <sup>64</sup>Cu, and it was developed for positron emission tomography (PET) imaging of tumors with overexpressed GRP receptors (GRP-R) (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>). <sup>64</sup>Cu is a positron emitter with a 19.3% abundance and a physical half-life (<i>t</i><sub>&#x000bd;</sub>) of 12.7 h.</p><p>The amphibian bombesin (BBN or BN), a peptide of 14 amino acids, is an analog of human GRP, a peptide of 27 amino acids, that binds to GRP-R with high affinity and specificity (<a class="bibr" href="#MP2346Cu64.EXTYLES.2" rid="MP2346Cu64.EXTYLES.2">2</a>, <a class="bibr" href="#MP2346Cu64.EXTYLES.3" rid="MP2346Cu64.EXTYLES.3">3</a>). Both GRP and BN share an amidated C-terminus sequence homology of seven amino acids, -Trp-Ala-Val-Gly-His-Leu-Met-NH<sub>2</sub>. BN-Like peptides have been shown to induce various biological responses in diverse tissues, including the central nervous system (CNS) and the gastrointestinal (GI) system (<a class="bibr" href="#MP2346Cu64.EXTYLES.4" rid="MP2346Cu64.EXTYLES.4">4</a>, <a class="bibr" href="#MP2346Cu64.EXTYLES.5" rid="MP2346Cu64.EXTYLES.5">5</a>). They also act as potential growth factors for both normal and neoplastic tissues. Specific BN receptors (BN-R) have been identified in CNS and GI tissues and a number of tumor cell lines. The BN-R superfamily includes at least four different subtypes, namely the GRP-R subtype (BB2), the neuromedin B receptor subtype (BB1), the BB3 subtype, and the BB4 subtype (<a class="bibr" href="#MP2346Cu64.EXTYLES.6" rid="MP2346Cu64.EXTYLES.6">6</a>). Overexpression of GRP-R in various human tumors (e.g., breast, prostate, lung, colon, ovarian, and pancreatic cancers) provides opportunities to image tumors with the use of specific molecular imaging agents designed to target the GRP-R (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>, <a class="bibr" href="#MP2346Cu64.EXTYLES.3" rid="MP2346Cu64.EXTYLES.3">3</a>, <a href="#MP2346Cu64.EXTYLES.7">7-9</a>).</p><p>There have been varying degrees of success in the current development of GRP-R&#x02013;targeted radiopharmaceuticals for diagnostic or therapeutic applications (<a class="bibr" href="#MP2346Cu64.EXTYLES.9" rid="MP2346Cu64.EXTYLES.9">9</a>). Various BN analogs have been labeled with <sup>99m</sup>Tc and <sup>111</sup>In for single-photon emission computed tomography (SPECT) imaging (<a class="bibr" href="#MP2346Cu64.EXTYLES.10" rid="MP2346Cu64.EXTYLES.10">10</a>, <a class="bibr" href="#MP2346Cu64.EXTYLES.11" rid="MP2346Cu64.EXTYLES.11">11</a>). BN Analogs labeled with <sup>68</sup>Ga, <sup>18</sup>F, <sup>86</sup>Y, or <sup>64</sup>Cu have been studied for PET imaging (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>, <a class="bibr" href="#MP2346Cu64.EXTYLES.12" rid="MP2346Cu64.EXTYLES.12">12</a>, <a class="bibr" href="#MP2346Cu64.EXTYLES.13" rid="MP2346Cu64.EXTYLES.13">13</a>). Breeman et al. (<a class="bibr" href="#MP2346Cu64.EXTYLES.14" rid="MP2346Cu64.EXTYLES.14">14</a>, <a class="bibr" href="#MP2346Cu64.EXTYLES.15" rid="MP2346Cu64.EXTYLES.15">15</a>) prepared two GRP-R agonists, diethylenetriamine pentaacetic acid (DTPA)-[Pro<sup>1</sup>,Tyr<sup>4</sup>]BN[1-14] and 1,4,7,10-tetraazacyclododecane-<i>N</i>,<i>N'</i>,<i>N''</i>,<i>N'''</i>-tetraacetic acid (DOTA)-[Pro<sup>1</sup>,Tyr<sup>4</sup>]BN[1-14] (MP2346), for radiometal labeling by replacing pGlu<sup>1</sup> and Leu<sup>4</sup> in the native BN with DTPA-Pro or DOTA-Pro and with Tyr, respectively. Both MP2346 and DTPA-[Pro<sup>1</sup>,Tyr<sup>4</sup>]BN[1-14] were readily labeled with <sup>111</sup>In and appeared to be promising radioligands for SPECT imaging. For PET imaging, Biddlecombe et al. (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>) prepared and evaluated <sup>64</sup>Cu-MP2346 and <sup>86</sup>Y-MP2346. The investigators reported distinct differences in the <i>in vivo</i> pharmacokinetics and tumor uptake of these two radioligands; the authors suggested that this may be the result of their differences in peptide-to-receptor affinity, overall chemical charge, and radiometal-chelate stability.</p></div><div id="MP2346Cu64.Synthesis"><h2 id="_MP2346Cu64_Synthesis_">Synthesis</h2><p>[<a href="/sites/entrez?Db=pubmed&#x00026;Cmd=DetailsSearch&#x00026;Term=(86Y-Bombesin)+OR+(gastrin-releasing+peptide+probe)" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubMed</a>]</p><p>Biddlecombe et al. (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>) reported the synthesis of <sup>64</sup>Cu-MP2346. The [Pro<sup>1</sup>,Tyr<sup>4</sup>]BN[1-14] peptide was synthesized by the standard 9-fluorenylmethyloxycarbonyl (Fmoc) solid-phase chemistry and Rink amide resin. The DOTA conjugation procedure with [Pro<sup>1</sup>,Tyr<sup>4</sup>]BN[1-14] was not discussed. The standard method that involved the sulfosuccinimidyl ester of DOTA was used (<a class="bibr" href="#MP2346Cu64.EXTYLES.16" rid="MP2346Cu64.EXTYLES.16">16</a>). The resulting MP2346 was purified with high-performance liquid chromatography (HPLC), and the mass was confirmed by mass spectrometry. The radiolabeling involved mixing MP2346 with <sup>64</sup>Cu chloride (<sup>64</sup>CuCl<sub>2</sub>) in ammonium acetate (pH 5.5). The reaction mixture was incubated at 80&#x000ba;C for 30 min. Reaction progress and purity were analyzed by analytical reverse-phase HPLC. On the basis of the HPLC analysis, the radiochemical yield and radiochemical purity were &#x0003e;99%. The specific activity was 36.6 MBq/&#x003bc;g (0.99 mCi/&#x003bc;g) or 72.5 GBq/&#x003bc;mol (1.958 Ci/&#x003bc;mol).</p></div><div id="MP2346Cu64.In_Vitro_Studies_Tes"><h2 id="_MP2346Cu64_In_Vitro_Studies_Tes_"><i>In Vitro</i> Studies: Testing in Cells and Tissues</h2><p>[<a href="/sites/entrez?Db=pubmed&#x00026;Cmd=DetailsSearch&#x00026;Term=((86Y-Bombesin)+OR+(gastrin-releasing+peptide+probe))+AND+(in+vitro)" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubMed</a>]</p><p>Biddlecombe et al. (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>) performed <i>in vitro</i> internalization studies (<i>n</i> = 3) of <sup>64</sup>Cu-MP2346 in human prostate adenocarcinoma PC-3 cells. The amount of specifically internalized <sup>64</sup>Cu-MP2346 was 121.83 &#x000b1; 33.74 fmol/mg at 15 min after incubation and increased steadily to 512.04 &#x000b1; 83.41 fmol/mg at 20 h. The amount of surface-bound radioactivity was &#x0003c;100 fmol/mg at all times, and the maximum internalized radioactivity was 535 fmol/mg. In comparison, the <sup>86</sup>Y-MP2346 had a maximum internalization of 1,945 fmol/mg.</p></div><div id="MP2346Cu64.Animal_Studies"><h2 id="_MP2346Cu64_Animal_Studies_">Animal Studies</h2><div id="MP2346Cu64.Rodents"><h3>Rodents</h3><p>[<a href="/sites/entrez?Db=pubmed&#x00026;Cmd=DetailsSearch&#x00026;Term=((86Y-Bombesin)+OR+(gastrin-releasing+peptide+probe))++AND+(mice)" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubMed</a>]</p><p>Biodistribution studies of <sup>64</sup>Cu-MP2346 were performed in nude mice (<i>n</i> = 5) bearing human prostate adenocarcinoma PC-3 tumors in their flanks (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>). Each mouse received 0.22&#x02013;0.27 kBq (8&#x02013;10 &#x003bc;Ci) radioactivity in 8&#x02013;10 ng of <sup>64</sup>Cu-MP2346 by i.v. administration. <sup>64</sup>Cu-MP2346 showed rapid clearance of radioactivity from the blood over 24 h. The tumor radioactivity levels in percentage injected dose per gram (% ID/g) were 0.793 &#x000b1; 0.181 (1 h), 0.421 &#x000b1; 0.214 (4 h), and 0.309 &#x000b1; 0.199 (24 h). The tumor/blood ratios were 6.192 (1 h), 5.511 (4 h), and 4.487 (24 h), and the tumor/muscle ratios were 2.718 (1 h), 4.996 (4 h), and 9.056 (24 h). In comparison, the tumor radioactivity levels and tumor/organ ratios of <sup>86</sup>Y-MP2346 were significantly higher (<i>P</i> &#x0003c; 0.0005). At 1 h, the radioactivity levels (% ID/g) for major organs were 0.128 &#x000b1; 0.0015 (blood), 0.426 &#x000b1; 0.117 (lung), 1.329 &#x000b1; 0.304 (liver), 6.148 &#x000b1; 1.067 (kidney), 0.316 &#x000b1; 0.255 (muscle), 0.160 &#x000b1; 0.048 (heart), 0.885 &#x000b1; 0.928 (bone), and 4.387 &#x000b1; 1.090 (pancreas). At 24 h, these levels changed to 0.069 &#x000b1; 0.007 (blood), 0.218 &#x000b1; 0.043 (lung), 1.089 &#x000b1; 0.429 (liver), 1.222 &#x000b1; 0.296 (kidney), 0.034 &#x000b1; 0.013 (muscle), 0.1865 &#x000b1; 0.024 (heart), 0.038 &#x000b1; 0.026 (bone), and 1.155 &#x000b1; 0.327 (pancreas). Blocking studies with preinjection of 100 &#x003bc;g of [Tyr<sup>4</sup>]-BN immediately before radioligand injection were performed, and the mice were euthanized 1 h after injection. Radioactivity levels of both the tumor and GRP-R&#x02013;rich pancreas decreased significantly (<i>P</i> &#x0003c; 0.0005) to 0.099 &#x000b1; 0.134% ID/g and 0.464 &#x000b1; 0.259% ID/g, respectively. Similar trends were observed in other GRP-R&#x02013;rich organs.</p><p>PET imaging with computed tomography coregistration of <sup>64</sup>Cu-MP2346 was conducted in mice bearing PC-3 tumors (<i>n</i> = 3) with a dose of 4.625 MBq (0.125 mCi) in 125 ng peptide by i.v. administration (<a class="bibr" href="#MP2346Cu64.EXTYLES.1" rid="MP2346Cu64.EXTYLES.1">1</a>). The images showed that the radioactivity distribution pattern was similar to that of the biodistribution studies. The tumor was clearly visualized for 1&#x02013;24 h. There was significant activity in the liver as shown by the biodistribution studies. The blocking study of mice coinjected with 100 &#x003bc;g [Tyr<sup>4</sup>]-BN and imaged side by side with mice that received only <sup>64</sup>Cu-MP2346 showed a significant reduction of tumor radioactivity. The measured standard uptake value of <sup>64</sup>Cu-MP2346 for the tumor at 1 h after injection decreased from ~1 (range, 0.8-1) in the study without blocking to ~0.4 (0.2&#x02013;0.5) with blocking (extrapolated from Figure 4).</p></div><div id="MP2346Cu64.Other_NonPrimate_Mam"><h3>Other Non-Primate Mammals</h3><p>[<a href="/sites/entrez?Db=pubmed&#x00026;Cmd=DetailsSearch&#x00026;Term=((86Y-Bombesin)+OR+(gastrin-releasing+peptide+probe))+AND+(primate+NOT+human)" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubMed</a>]</p><p>No publication is currently available.</p></div><div id="MP2346Cu64.NonHuman_Primates"><h3>Non-Human Primates</h3><p>[<a href="/sites/entrez?Db=pubmed&#x00026;Cmd=DetailsSearch&#x00026;Term=((86Y-Bombesin)+OR+(gastrin-releasing+peptide+probe))+AND+(primate+NOT+human)" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubMed</a>]</p><p>No publication is currently available.</p></div></div><div id="MP2346Cu64.Human_Studies"><h2 id="_MP2346Cu64_Human_Studies_">Human Studies</h2><p>[<a href="/sites/entrez?Db=pubmed&#x00026;Cmd=DetailsSearch&#x00026;Term=((86Y-Bombesin)+OR+(gastrin-releasing+peptide+probe))+AND+(humans)" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubMed</a>]</p><p>No publication is currently available.</p></div><div id="MP2346Cu64.NIH_Support"><h2 id="_MP2346Cu64_NIH_Support_">NIH Support</h2><p>NCI R24 CA86307, NIH/NCI SAIRP grant R24 CA83060, NCI Cancer Center Support Grant 1 P30 CA91842.</p></div><div id="MP2346Cu64.references"><h2 id="_MP2346Cu64_references_">References</h2><dl class="temp-labeled-list"><dl class="bkr_refwrap"><dt>1.</dt><dd><div class="bk_ref" id="MP2346Cu64.EXTYLES.1">Biddlecombe G.B. , Rogers B.E. , de Visser M. , Parry J.J. , de Jong M. , Erion J.L. , Lewis J.S. Molecular imaging of gastrin-releasing peptide receptor-positive tumors in mice using 64Cu- and 86Y-DOTA-(Pro1,Tyr4)-bombesin(1-14). <span><span class="ref-journal">Bioconjug Chem. </span>2007;<span class="ref-vol">
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</span>(2):247&ndash;58.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/12552343" ref="pagearea=cite-ref&amp;targetsite=entrez&amp;targetcat=link&amp;targettype=pubmed">PubMed<span class="bk_prnt">: 12552343</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>9.</dt><dd><div class="bk_ref" id="MP2346Cu64.EXTYLES.9">Smith C.J. , Volkert W.A. , Hoffman T.J. Gastrin releasing peptide (GRP) receptor targeted radiopharmaceuticals: a concise update. <span><span class="ref-journal">Nucl Med Biol. </span>2003;<span class="ref-vol">
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</span>(5):657&ndash;63.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/10521803" ref="pagearea=cite-ref&amp;targetsite=entrez&amp;targetcat=link&amp;targettype=pubmed">PubMed<span class="bk_prnt">: 10521803</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>15.</dt><dd><div class="bk_ref" id="MP2346Cu64.EXTYLES.15">Breeman W.A. , de Jong M. , Erion J.L. , Bugaj J.E. , Srinivasan A. , Bernard B.F. , Kwekkeboom D.J. , Visser T.J. , Krenning E.P. Preclinical comparison of (111)In-labeled DTPA- or DOTA-bombesin analogs for receptor-targeted scintigraphy and radionuclide therapy. <span><span class="ref-journal">J Nucl Med. </span>2002;<span class="ref-vol">
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</span>(12):1650&ndash;6.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/12468515" ref="pagearea=cite-ref&amp;targetsite=entrez&amp;targetcat=link&amp;targettype=pubmed">PubMed<span class="bk_prnt">: 12468515</span></a>]</div></dd></dl><dl class="bkr_refwrap"><dt>16.</dt><dd><div class="bk_ref" id="MP2346Cu64.EXTYLES.16">Chen X. , Park R. , Hou Y. , Tohme M. , Shahinian A.H. , Bading J.R. , Conti P.S. microPET and autoradiographic imaging of GRP receptor expression with 64Cu-DOTA-[Lys3]bombesin in human prostate adenocarcinoma xenografts. <span><span class="ref-journal">J Nucl Med. </span>2004;<span class="ref-vol">
<strong>45</strong>
</span>(8):1390&ndash;7.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/15299066" ref="pagearea=cite-ref&amp;targetsite=entrez&amp;targetcat=link&amp;targettype=pubmed">PubMed<span class="bk_prnt">: 15299066</span></a>]</div></dd></dl></dl></div><div><dl class="temp-labeled-list small"><dl class="bkr_refwrap"><dt></dt><dd><div><p class="no_top_margin"><div>This MICAD chapter is not included in the Open Access Subset, because it was authored / co-authored by one or more investigators who was not a member of the MICAD staff.</div></p></div></dd></dl></dl></div><div id="bk_toc_contnr"></div></div></div><div class="fm-sec"><h2 id="_NBK23640_pubdet_">Publication Details</h2><h3>Author Information and Affiliations</h3><div class="contrib half_rhythm"><span itemprop="author">Kenneth T. Cheng</span>, PhD<div class="affiliation small">
National Center for Biotechnology Information, NLM, NIH, Bethesda, MD,
<span class="before-email-separator"></span><span class="email-label">Email: </span><a href="mailto:dev@null" data-email="vog.hin.mln.ibcn@dacim" class="oemail">vog.hin.mln.ibcn@dacim</a>
</div></div><div class="contrib half_rhythm"><span itemprop="author">Jason S. Lewis</span>, PhD<div class="affiliation small">
Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO, Corresponding Author,
<span class="before-email-separator"></span><span class="email-label">Email: </span><a href="mailto:dev@null" data-email="ude.ltsuw@siwel.s.j" class="oemail">ude.ltsuw@siwel.s.j</a>
</div></div><div class="contrib half_rhythm"><span itemprop="author">Gr&#x000e1;inne B. Biddlecombe</span>, MS<div class="affiliation small">
Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO,
<span class="before-email-separator"></span><span class="email-label">Email: </span><a href="mailto:dev@null" data-email="ude.ltsuw.rim@gebmocelddib" class="oemail">ude.ltsuw.rim@gebmocelddib</a>
</div></div><h3>Publication History</h3><p class="small">Created: <span itemprop="datePublished">August 16, 2007</span>; Last Update: <span itemprop="dateModified">January 8, 2008</span>.</p><h3>Copyright</h3><div><div class="half_rhythm"><a href="/books/about/copyright/">Copyright Notice</a></div></div><h3>Publisher</h3><p><a href="http://www.ncbi.nlm.nih.gov/" ref="pagearea=page-banner&amp;targetsite=external&amp;targetcat=link&amp;targettype=publisher">National Center for Biotechnology Information (US)</a>, Bethesda (MD)</p><h3>NLM Citation</h3><p>Cheng KT, Lewis JS, Biddlecombe GB. 64Cu-DOTA-[Pro1,Tyr4]-Bombesin[1-14] 2007 Aug 16 [Updated 2008 Jan 8]. In: Molecular Imaging and Contrast Agent Database (MICAD) [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2004-2013. <span class="bk_cite_avail"></span></p></div><div class="small-screen-prev"><a href="/books/n/micad/Cu64-DTPA-ProT/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M75,30 c-80,60 -80,0 0,60 c-30,-60 -30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Prev</text></svg></a></div><div class="small-screen-next"><a href="/books/n/micad/TrastFv-FcDM64Cu/?report=reader"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 100 100" preserveAspectRatio="none"><path d="M25,30c80,60 80,0 0,60 c30,-60 30,0 0,-60"></path><text x="20" y="28" textLength="60" style="font-size:25px">Next</text></svg></a></div></article><article data-type="table-wrap" id="figobMP2346Cu64T1"><div id="MP2346Cu64.T1" class="table"><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK23640/table/MP2346Cu64.T1/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__MP2346Cu64.T1_lrgtbl__"><table><tbody><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Chemical name:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;"><sup>64</sup>Cu-DOTA-[Pro<sup>1</sup>,Tyr<sup>4</sup>]-Bombesin[1-14]</td><td rowspan="9" colspan="1" style="text-align:left;vertical-align:middle;">
<a href="https://pubchem.ncbi.nlm.nih.gov/substance/26697031" title="View this structure in PubChem" class="img_link" ref="pagearea=body&amp;targetsite=entrez&amp;targetcat=link&amp;targettype=pubchem"><img src="https://pubchem.ncbi.nlm.nih.gov/image/imgsrv.fcgi?t=l&amp;sid=26697031" alt="image 26697031 in the ncbi pubchem database" /></a>
</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Abbreviated name:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;"><sup>64</sup>Cu-MP2346</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Synonym:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;"><sup>64</sup>Cu-Bombesin, <sup>64</sup>Cu-BN, <sup>64</sup>Cu-BNN</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Agent Category:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Peptide</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Target:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gastrin-releasing peptide receptor (GRP-R)</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Target Category:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Receptor binding</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Method of detection:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Positron emission tomography (PET) imaging</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Source of signal/ contrast:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;"><sup>64</sup>Cu</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Activation:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">No</td></tr><tr><td rowspan="1" colspan="1" style="text-align:right;vertical-align:top;">
<b>Studies:</b>
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">
<ul class="simple-list"><li class="half_rhythm"><div>
<img alt="Checkbox" src="/corehtml/pmc/css/bookshelf/2.26/img/studies.checkbox.png" />
<i>In vitro</i>
</div></li></ul>
<ul class="simple-list"><li class="half_rhythm"><div>
<img alt="Checkbox" src="/corehtml/pmc/css/bookshelf/2.26/img/studies.checkbox.png" /> Rodents
</div></li></ul>
<br />
</td><td rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Click on the above structure for additional information in <a href="http://pubchem.ncbi.nlm.nih.gov" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">PubChem</a>.<br />Click on <a href="/entrez/viewer.fcgi?db=protein&#x00026;id=55584176" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">protein</a>, <a href="/entrez/viewer.fcgi?db=nuccore&#x00026;id=61677286" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">nucleotide</a> (RefSeq), and <a href="/sites/entrez?Db=gene&#x00026;Cmd=ShowDetailView&#x00026;TermToSearch=2925&#x00026;ordinalpos=5&#x00026;itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum" ref="pagearea=body&amp;targetsite=external&amp;targetcat=link&amp;targettype=uri">gene</a> for more information about BN and GRP.</td></tr></tbody></table></div></div></article></div><div id="jr-scripts"><script src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/libs.min.js"> </script><script src="/corehtml/pmc/jatsreader/ptpmc_3.22/js/jr.min.js"> </script></div></div>
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