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<title>Glycosphingolipid-glycan analysis of human whole serum/plasma based on an endoglycoceramidase digestion - Glycoscience Protocols (GlycoPODv2) - NCBI Bookshelf</title>
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<meta name="robots" content="INDEX,FOLLOW,NOARCHIVE" /><meta name="citation_inbook_title" content="Glycoscience Protocols (GlycoPODv2) [Internet]" /><meta name="citation_title" content="Glycosphingolipid-glycan analysis of human whole serum/plasma based on an endoglycoceramidase digestion" /><meta name="citation_publisher" content="Japan Consortium for Glycobiology and Glycotechnology" /><meta name="citation_date" content="2022/03/22" /><meta name="citation_author" content="Hisatoshi Hanamatsu" /><meta name="citation_author" content="Jun-ichi Furukawa" /><meta name="citation_pmid" content="37590617" /><meta name="citation_fulltext_html_url" content="https://www.ncbi.nlm.nih.gov/books/NBK593871/" /><meta name="citation_keywords" content="glycosphingolipid" /><meta name="citation_keywords" content="endoglycoceramidase I" /><meta name="citation_keywords" content="glycoblotting" /><meta name="citation_keywords" content="human serum" /><meta name="citation_keywords" content="human plasma" /><link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" /><meta name="DC.Title" content="Glycosphingolipid-glycan analysis of human whole serum/plasma based on an endoglycoceramidase digestion" /><meta name="DC.Type" content="Text" /><meta name="DC.Publisher" content="Japan Consortium for Glycobiology and Glycotechnology" /><meta name="DC.Contributor" content="Hisatoshi Hanamatsu" /><meta name="DC.Contributor" content="Jun-ichi Furukawa" /><meta name="DC.Date" content="2022/03/22" /><meta name="DC.Identifier" content="https://www.ncbi.nlm.nih.gov/books/NBK593871/" /><meta name="description" content="Glycosphingolipids (GSLs) are lipid molecules linked to carbohydrate units and form the plasma membrane lipid raft, which is an important membrane region associated with numerous life events (1). These GSL molecules consequently affect the pathophysiology and pathogenesis of various diseases (2–4). Thus, altered glycosylation on GSLs may be promising for disease-related biomarker discovery. Previously, we reported that quantitative GSL-glycome analysis of human serum based on an endoglycoceramidase (EGCase) digestion and glycoblotting method (5,6). Here, we will describe the analytical protocol of GSL-glycans in human serum/plasma in detail." /><meta name="og:title" content="Glycosphingolipid-glycan analysis of human whole serum/plasma based on an endoglycoceramidase digestion" /><meta name="og:type" content="book" /><meta name="og:description" content="Glycosphingolipids (GSLs) are lipid molecules linked to carbohydrate units and form the plasma membrane lipid raft, which is an important membrane region associated with numerous life events (1). These GSL molecules consequently affect the pathophysiology and pathogenesis of various diseases (2–4). Thus, altered glycosylation on GSLs may be promising for disease-related biomarker discovery. Previously, we reported that quantitative GSL-glycome analysis of human serum based on an endoglycoceramidase (EGCase) digestion and glycoblotting method (5,6). Here, we will describe the analytical protocol of GSL-glycans in human serum/plasma in detail." /><meta name="og:url" content="https://www.ncbi.nlm.nih.gov/books/NBK593871/" /><meta name="og:site_name" content="NCBI Bookshelf" /><meta name="og:image" content="https://www.ncbi.nlm.nih.gov/corehtml/pmc/pmcgifs/bookshelf/thumbs/th-glycopodv2-lrg.png" /><meta name="twitter:card" content="summary" /><meta name="twitter:site" content="@ncbibooks" /><meta name="bk-non-canon-loc" content="/books/n/glycopodv2/g145-humanwholeserum/" /><link rel="canonical" href="https://www.ncbi.nlm.nih.gov/books/NBK593871/" /><link rel="stylesheet" href="/corehtml/pmc/css/figpopup.css" type="text/css" media="screen" /><link rel="stylesheet" href="/corehtml/pmc/css/bookshelf/2.26/css/books.min.css" type="text/css" /><link rel="stylesheet" href="/corehtml/pmc/css/bookshelf/2.26/css/books_print.min.css" type="text/css" /><style type="text/css">p a.figpopup{display:inline !important} .bk_tt {font-family: monospace} .first-line-outdent .bk_ref {display: inline} </style><script type="text/javascript" src="/corehtml/pmc/js/jquery.hoverIntent.min.js"> </script><script type="text/javascript" src="/corehtml/pmc/js/common.min.js?_=3.18"> </script><script type="text/javascript">window.name="mainwindow";</script><script type="text/javascript" src="/corehtml/pmc/js/bookshelf/2.26/book-toc.min.js"> </script><script type="text/javascript" src="/corehtml/pmc/js/bookshelf/2.26/books.min.js"> </script>
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<div class="pre-content"><div><div class="bk_prnt"><p class="small">NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.</p><p>Nishihara S, Angata K, Aoki-Kinoshita KF, et al., editors. Glycoscience Protocols (GlycoPODv2) [Internet]. Saitama (JP): Japan Consortium for Glycobiology and Glycotechnology; 2021-. </p></div></div></div>
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<div class="main-content lit-style" itemscope="itemscope" itemtype="http://schema.org/CreativeWork"><div class="meta-content fm-sec"><h1 id="_NBK593871_"><span class="title" itemprop="name">Glycosphingolipid-glycan analysis of human whole serum/plasma based on an endoglycoceramidase digestion</span></h1><div class="contrib half_rhythm"><span itemprop="author">Hisatoshi Hanamatsu</span>, Dr<div class="affiliation small">Hokkaido
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Univ<div><span class="email-label">Email: </span><a href="mailto:dev@null" data-email="pj.ca.iadukoh.dem@ustamanah_h" class="oemail">pj.ca.iadukoh.dem@ustamanah_h</a></div></div></div><div class="contrib half_rhythm"><span itemprop="author">Jun-ichi Furukawa</span>, Dr<div class="affiliation small">Hokkaido
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Univ<div><span class="email-label">Email: </span><a href="mailto:dev@null" data-email="pj.ca.iadukoh.dem@uruf_j" class="oemail">pj.ca.iadukoh.dem@uruf_j</a></div></div><div class="small">Corresponding author.</div></div><p class="small">Created: <span itemprop="datePublished">September 29, 2021</span>; Last Revision: <span itemprop="dateModified">March 22, 2022</span>.</p></div><div class="body-content whole_rhythm" itemprop="text"><div id="g145-humanwholeserum.Introduction"><h2 id="_g145-humanwholeserum_Introduction_">Introduction</h2><p>Glycosphingolipids (GSLs) are lipid molecules linked to carbohydrate units and form the plasma membrane lipid raft, which is an important membrane region associated with numerous life events (<a class="bk_pop" href="#g145-humanwholeserum.REF.1">1</a>). These GSL molecules consequently affect the pathophysiology and pathogenesis of various diseases (<a class="bk_pop" href="#g145-humanwholeserum.REF.2">2</a>–<a class="bk_pop" href="#g145-humanwholeserum.REF.4">4</a>). Thus, altered glycosylation on GSLs may be promising for disease-related biomarker discovery. Previously, we reported that quantitative GSL-glycome analysis of human serum based on an endoglycoceramidase (EGCase) digestion and glycoblotting method (<a class="bk_pop" href="#g145-humanwholeserum.REF.5">5</a>,<a class="bk_pop" href="#g145-humanwholeserum.REF.6">6</a>). Here, we will describe the analytical protocol of GSL-glycans in human serum/plasma in detail.</p></div><div id="g145-humanwholeserum.Protocol"><h2 id="_g145-humanwholeserum_Protocol_">Protocol</h2><p>The following protocol describes the analysis of GSL-glycans in serum/plasma by MALDI-TOF-MS: 1) quenching of blood glucose by glucose oxidase; 2) release of GSL-glycans by EGCase I; and 3) glycan purification and highly sensitive labeling by glycoblotting purification. The streamlined protocol for GSL-glycan profiles is shown in <a class="figpopup" href="/books/NBK593871/figure/g145-humanwholeserum.F1/?report=objectonly" target="object" rid-figpopup="figg145humanwholeserumF1" rid-ob="figobg145humanwholeserumF1">Figure 1</a>. GSL-glycans detected in various blood type human serums are summarized in <a class="figpopup" href="/books/NBK593871/figure/g145-humanwholeserum.F2/?report=objectonly" target="object" rid-figpopup="figg145humanwholeserumF2" rid-ob="figobg145humanwholeserumF2">Figure 2</a> and <a class="figpopup" href="/books/NBK593871/table/g145-humanwholeserum.T.list_of_gslglycan/?report=objectonly" target="object" rid-figpopup="figg145humanwholeserumTlistofgslglycan" rid-ob="figobg145humanwholeserumTlistofgslglycan">Table 1</a>.</p><div id="g145-humanwholeserum.Materials"><h3>Materials</h3><dl class="temp-labeled-list"><dt>1.</dt><dd><p class="no_top_margin">Human serum (Sigma-Aldrich, St. Louis, MO, Cat #M4522)</p></dd><dt>2.</dt><dd><p class="no_top_margin">Glucose oxidase (Sigma-Aldrich, Cat #345386): 2.1 U/mL in 100 mM of phosphate buffer, pH 7.0</p></dd><dt>3.</dt><dd><p class="no_top_margin">Acetate buffer: 1.52 M sodium acetate (Sigma-Aldrich, Cat #S2889), pH 3.4</p></dd><dt>4.</dt><dd><p class="no_top_margin"><i>r</i>Endoglycoceramidase I (rEGCase I, Tokyo Chemical Industry, Tokyo, Japan, Cat #R0240)</p></dd><dt>5.</dt><dd><p class="no_top_margin">Cold ethanol at −30°C (FUJIFILM Wako Pure Chemical, Osaka, Japan, Cat #057-00451)</p></dd><dt>6.</dt><dd><p class="no_top_margin">MultiScreen Solvinert 0.45 μm Low-Binding Hydrophilic Polytetrafluoroethylene (PTFE) filter plate (Millipore, Billerica, MA, Cat #MSRLN0410)</p></dd><dt>7.</dt><dd><p class="no_top_margin">Filter plate seal (Greiner Bio-One, Frickenhausen, Germany, Cat #J676060)</p></dd><dt>8.</dt><dd><p class="no_top_margin">BlotGlyco® bead suspension in water (10 mg/mL) (Sumitomo Bakelite Co., Ltd., Tokyo, Japan, Cat #BS-45408)</p></dd><dt>9.</dt><dd><p class="no_top_margin">10 μM of A2GN1 (Neu5Ac2Gal2GlcNAc2 + Man3GlcNAc1) (Tokyo Chemical Industry, Cat #D4065)</p></dd><dt>10.</dt><dd><p class="no_top_margin">Acetonitrile (FUJIFILM Wako Pure Chemical, Cat #018-19853)</p></dd><dt>11.</dt><dd><p class="no_top_margin">2% Acetic acid (FUJIFILM Wako Pure Chemical, Cat #012-00245) in acetonitrile</p></dd><dt>12.</dt><dd><p class="no_top_margin">2 M guanidine hydrochloride (FUJIFILM Wako Pure Chemical, Cat #077-02435)</p></dd><dt>13.</dt><dd><p class="no_top_margin">1% triethylamine (FUJIFILM Wako Pure Chemical, Cat #202-02646) in methanol</p></dd><dt>14.</dt><dd><p class="no_top_margin">10% acetic anhydride (FUJIFILM Wako Pure Chemical, Cat #011-00271) in methanol</p></dd><dt>15.</dt><dd><p class="no_top_margin">10 mM of hydrochloric acid (FUJIFILM Wako Pure Chemical, Cat #192-02175)</p></dd><dt>16.</dt><dd><p class="no_top_margin">Methanol (FUJIFILM Wako Pure Chemical, Cat #134-14523)</p></dd><dt>17.</dt><dd><p class="no_top_margin">1,4-Dioxane (FUJIFILM Wako Pure Chemical, Cat #042-03761)</p></dd><dt>18.</dt><dd><p class="no_top_margin">Esterification solution: 100 mM of 1-methyl-3-p-tolyltriazene (Tokyo Chemical Industry, Cat #M0641) in 1,4-dioxane</p></dd><dt>19.</dt><dd><p class="no_top_margin">Nα-((aminooxy)acetyl)tryptophanylarginine methyl ester (aoWR)</p></dd><dt>20.</dt><dd><p class="no_top_margin">Hydrophilic interaction liquid chromatography (HILIC) micro-elution plate (Waters, Milford, MA, Cat #186002780)</p></dd><dt>21.</dt><dd><p class="no_top_margin">HILIC prewash solution: 99% H<sub>2</sub>O and 1% acetic acid</p></dd><dt>22.</dt><dd><p class="no_top_margin">HILIC wash solution: 95% acetonitrile, 4% H<sub>2</sub>O, and 1% acetic acid</p></dd><dt>23.</dt><dd><p class="no_top_margin">HILIC loading solution: 99% acetonitrile and 1% acetic acid</p></dd><dt>24.</dt><dd><p class="no_top_margin">HILIC elution solution: 5% acetonitrile, 94% H<sub>2</sub>O, and 1% acetic acid</p></dd><dt>25.</dt><dd><p class="no_top_margin">Matrix solution: 10 mg/mL of 2,5-dihydrobenzoic acid (Sigma-Aldrich, Cat #149357) in 30% acetonitrile</p></dd></dl></div><div id="g145-humanwholeserum.Instruments"><h3>Instruments</h3><dl class="temp-labeled-list"><dt>1.</dt><dd><p class="no_top_margin">High-speed refrigerated microcentrifuge (MX-307: TOMY, Tokyo, Japan)</p></dd><dt>2.</dt><dd><p class="no_top_margin">Centrifugal evaporator (FDU-2110: EYELA, Tokyo, Japan)</p></dd><dt>3.</dt><dd><p class="no_top_margin">Vacuum manifold for 96-well plates (Waters)</p></dd><dt>4.</dt><dd><p class="no_top_margin">Ultraflex II TOF/TOF MS instrument (Bruker Daltonics, Bremen, Germany)</p></dd><dt>5.</dt><dd><p class="no_top_margin">MALDI polished steel target plate (Bruker Daltonics, Cat #8280781)</p></dd><dt>6.</dt><dd><p class="no_top_margin">FlexAnalysis 3.0 software (Bruker Daltonics)</p></dd></dl></div><div id="g145-humanwholeserum.Methods"><h3>Methods</h3><dl class="temp-labeled-list"><dt>1.</dt><dd><p class="no_top_margin">Glucose oxidation</p><dl class="temp-labeled-list"><dt>a.</dt><dd><p class="no_top_margin">20–200 μL of human serum/plasma.</p></dd><dt>b.</dt><dd><p class="no_top_margin">Add 1–10 μL of acetate buffer to human serum and mix with a vortex mixer (<b>Note 1</b>).</p></dd><dt>c.</dt><dd><p class="no_top_margin">Add 10 μL of glucose oxidase and mix with a vortex mixer (<b>Note 2</b>).</p></dd><dt>d.</dt><dd><p class="no_top_margin">Incubate at 37°C for 16 h.</p></dd></dl></dd><dt>2.</dt><dd><p class="no_top_margin">GSL-glycan preparation</p><dl class="temp-labeled-list"><dt>a.</dt><dd><p class="no_top_margin">Add 2 μL of rEGCase I to 40 uL of oxidated human serum.</p></dd><dt>b.</dt><dd><p class="no_top_margin">Incubate at 37°C for 16 h</p></dd><dt>c.</dt><dd><p class="no_top_margin">Add 160 μL of cold ethanol (4-fold) and mix with a vortex mixer (<b>Note 3</b>).</p></dd><dt>d.</dt><dd><p class="no_top_margin">Centrifuge the samples at 14,000 g at 4°C for 20 min.</p></dd><dt>e.</dt><dd><p class="no_top_margin">Collect the supernatants containing GSL-glycans in 1.5-mL tubes.</p></dd><dt>f.</dt><dd><p class="no_top_margin">Dry the supernatants with a centrifugal evaporator.</p></dd><dt>g.</dt><dd><p class="no_top_margin">Add 50 μL of H<sub>2</sub>O and dissolve by sonication in a water bath for 30 s.</p></dd><dt>h.</dt><dd><p class="no_top_margin">Perform glycoblotting using half of the prepared sample.</p></dd></dl></dd><dt>3.</dt><dd><p class="no_top_margin">Glycoblotting for MALDI-TOF MS</p><dl class="temp-labeled-list"><dt>a.</dt><dd><p class="no_top_margin">Dispense 5 mg of BlotGlyco® beads on a filter plate (<b>Note 4</b>).</p></dd><dt>b.</dt><dd><p class="no_top_margin">Set the filter plate into a vacuum manifold.</p></dd><dt>c.</dt><dd><p class="no_top_margin">Add 25 μL of prepared GSL-glycan samples to each well of the filter plate.</p></dd><dt>d.</dt><dd><p class="no_top_margin">Add 2 μL of 10 μM of A2GN1 as an internal standard.</p></dd><dt>e.</dt><dd><p class="no_top_margin">Add 225 μL of 2% acetic acid in acetonitrile (<b>Note 5</b>).</p></dd><dt>f.</dt><dd><p class="no_top_margin">Incubate at 80°C for 60 min (<b>Note 6</b>).</p></dd><dt>g.</dt><dd><p class="no_top_margin">Set the filter plate into a vacuum manifold.</p></dd><dt>h.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of 2 M guanidine hydrochloride and repeat twice (<b>Note 7</b>).</p></dd><dt>i.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of H<sub>2</sub>O and repeat twice.</p></dd><dt>j.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of 1% triethylamine in methanol and repeat twice.</p></dd><dt>k.</dt><dd><p class="no_top_margin">Add 100 μL of 10% acetic anhydride in methanol and stand for 30 min (<b>Note 8</b>).</p></dd><dt>l.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of 10 mM of hydrochloric acid and repeat twice.</p></dd><dt>m.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of methanol and repeat twice.</p></dd><dt>n.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of 1,4-dioxane and repeat twice.</p></dd><dt>o.</dt><dd><p class="no_top_margin">Add 100 μL of esterification solution and incubate at 60°C for 70 min.</p></dd><dt>p.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of 1,4-dioxane and repeat twice.</p></dd><dt>q.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of methanol and repeat twice.</p></dd><dt>r.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of H<sub>2</sub>O and repeat twice.</p></dd><dt>s.</dt><dd><p class="no_top_margin">Add 20 μL of 20 mM of aoWR (<b>Note 9</b>).</p></dd><dt>t.</dt><dd><p class="no_top_margin">Add 180 μL of 2% acetic acid in acetonitrile.</p></dd><dt>u.</dt><dd><p class="no_top_margin">Incubate at 80°C for 45 min.</p></dd><dt>v.</dt><dd><p class="no_top_margin">Add 100 μL of H<sub>2</sub>O and elute aoWR-labeled glycans.</p></dd></dl></dd><dt>4.</dt><dd><p class="no_top_margin">aoWR-labeled glycan purification</p><dl class="temp-labeled-list"><dt>a.</dt><dd><p class="no_top_margin">Wash the well of the HILIC micro-elution plate with 200 μL of HILIC prewash solution and repeat.</p></dd><dt>b.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of HILIC wash solution and repeat.</p></dd><dt>c.</dt><dd><p class="no_top_margin">Add 450 μL of HILIC loading solution to 50 μL of the aoWR-labeled glycan sample (Step 3v) and transfer to the HILIC micro-elution plate.</p></dd><dt>d.</dt><dd><p class="no_top_margin">Wash by adding 200 μL of HILIC wash solution and repeat.</p></dd><dt>e.</dt><dd><p class="no_top_margin">Elute with 100 μL of HILIC elution solution.</p></dd><dt>f.</dt><dd><p class="no_top_margin">Analyze by MALDI-TOF MS.</p></dd></dl></dd><dt>5.</dt><dd><p class="no_top_margin">MALDI-TOF MS analysis</p><dl class="temp-labeled-list"><dt>a.</dt><dd><p class="no_top_margin">Mix 0.5 μL of the sample (Step 4e) and 0.5 μL of the matrix solution on the target plate.</p></dd><dt>b.</dt><dd><p class="no_top_margin">Dry the spots on the target plate at room temperature.</p></dd><dt>c.</dt><dd><p class="no_top_margin">Load the target plate into the instrument and select the appropriate MS method.</p></dd><dt>d.</dt><dd><p class="no_top_margin">Run the instrument in the reflector mode.</p></dd><dt>e.</dt><dd><p class="no_top_margin">Collect the MS data with 1,000–2,000 laser shots per sample and sum the collected data.</p></dd><dt>f.</dt><dd><p class="no_top_margin">Quantify the detected glycans using the area of the internal standard.</p></dd></dl></dd></dl></div><div id="g145-humanwholeserum.Notes"><h3>Notes</h3><dl class="temp-labeled-list"><dt>1.</dt><dd><p class="no_top_margin">To achieve the optimal pH for oxidizing glucose catalyzed by glucose oxidase, a 1/20 volume of acetate buffer was added to serum.</p></dd><dt>2.</dt><dd><p class="no_top_margin">A high blood glucose concentration is apparently undesirable measuring GSL-glycans in serum based on glycoblotting procedure utilized chemoselective ligation between aldehyde at the reducing ends of glycans and hydrazide group of glycan capture beads. The oxidation of D-glucose to D-glucono-δ-lactone enable us to markedly decrease the signal according to aoWR-labeled glucose.</p></dd><dt>3.</dt><dd><p class="no_top_margin">Cooling overnight is not a problem depending on the progress of the experiment; cooling for at least 3 h is mandatory.</p></dd><dt>4.</dt><dd><p class="no_top_margin">Cover the unused well with a plate seal and dispense 500 μL of the BlotGlyco® bead suspension (10 mg/mL) into a well.</p></dd><dt>5.</dt><dd><p class="no_top_margin">Add nine times the amount of 2% acetic acid in acetonitrile to the filter plate well.</p></dd><dt>6.</dt><dd><p class="no_top_margin">Continue heating until the solvent is completely dry.</p></dd><dt>7.</dt><dd><p class="no_top_margin">After the last wash with various solvents, wipe the bottom of the filter plate with a paper towel.</p></dd><dt>8.</dt><dd><p class="no_top_margin">Freshly prepare 10% acetic anhydride in methanol.</p></dd><dt>9.</dt><dd><p class="no_top_margin">aoWR was prepared as described previously (<a class="bk_pop" href="#g145-humanwholeserum.REF.7">7</a>).</p></dd></dl></div></div><div id="g145-humanwholeserum.References"><h2 id="_g145-humanwholeserum_References_">References</h2><dl class="temp-labeled-list"><dt>1.</dt><dd><div class="bk_ref" id="g145-humanwholeserum.REF.1">Lingwood D, Simons K. Lipid rafts as a membrane-organizing principle. <span><span class="ref-journal">Science. </span>2010 Jan 1;<span class="ref-vol">327</span>(5961):46–50.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/20044567" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 20044567</span></a>] [<a href="http://dx.crossref.org/10.1126/science.1174621" ref="pagearea=cite-ref&targetsite=external&targetcat=link&targettype=uri">CrossRef</a>]</div></dd><dt>2.</dt><dd><div class="bk_ref" id="g145-humanwholeserum.REF.2">Platt FM. Sphingolipid lysosomal storage disorders. <span><span class="ref-journal">Nature. </span>2014 Jun 5;<span class="ref-vol">510</span>(7503):68–75.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/24899306" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 24899306</span></a>] [<a href="http://dx.crossref.org/10.1038/nature13476" ref="pagearea=cite-ref&targetsite=external&targetcat=link&targettype=uri">CrossRef</a>]</div></dd><dt>3.</dt><dd><div class="bk_ref" id="g145-humanwholeserum.REF.3">Ryland LK, Fox TE, Liu X, Loughran TP, Kester M. Dysregulation of sphingolipid metabolism in cancer. <span><span class="ref-journal">Cancer Biol Ther. </span>2011 Jan 15;<span class="ref-vol">11</span>(2):138–49.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/21209555" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 21209555</span></a>] [<a href="http://dx.crossref.org/10.4161/cbt.11.2.14624" ref="pagearea=cite-ref&targetsite=external&targetcat=link&targettype=uri">CrossRef</a>]</div></dd><dt>4.</dt><dd><div class="bk_ref" id="g145-humanwholeserum.REF.4">Inokuchi J. GM3 and diabetes. <span><span class="ref-journal">Glycoconj J. </span>2014 Apr;<span class="ref-vol">31</span>(3):193–7.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/24399479" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 24399479</span></a>] [<a href="http://dx.crossref.org/10.1007/s10719-013-9516-4" ref="pagearea=cite-ref&targetsite=external&targetcat=link&targettype=uri">CrossRef</a>]</div></dd><dt>5.</dt><dd><div class="bk_ref" id="g145-humanwholeserum.REF.5">Furukawa JI, Sakai S, Yokota I, Okada K, Hanamatsu H, Kobayashi T, Yoshida Y, Higashino K, Tamura T, Igarashi Y, Shinohara Y. Quantitative GSL-glycome analysis of human whole serum based on an EGCase digestion and glycoblotting method. <span><span class="ref-journal">Journal of Lipid Research. </span>2015 Dec;<span class="ref-vol">56</span>(12):2399–407.</span> [<a href="/pmc/articles/PMC4655979/" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pmc">PMC free article<span class="bk_prnt">: PMC4655979</span></a>] [<a href="https://pubmed.ncbi.nlm.nih.gov/26420879" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 26420879</span></a>] [<a href="http://dx.crossref.org/10.1194/jlr.D062083" ref="pagearea=cite-ref&targetsite=external&targetcat=link&targettype=uri">CrossRef</a>]</div></dd><dt>6.</dt><dd><div class="bk_ref" id="g145-humanwholeserum.REF.6">Furukawa JI, Hanamatsu H, Yokota I, Hirayama M, Ando T, Kobayashi H, Ohnishi S, Miura N, Okada K, Sakai S, Yuyama K, Igarashi Y, Ito M, Shinohara Y, Sakamoto N. Comprehensive glycomic approach reveals novel low-molecular-weight blood group-specific glycans in serum and cerebrospinal fluid. <span><span class="ref-journal">J Proteome Res. </span>2021 May 7;<span class="ref-vol">20</span>(5):2812–2822.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/33719461" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 33719461</span></a>] [<a href="http://dx.crossref.org/10.1021/acs.jproteome.1c00056" ref="pagearea=cite-ref&targetsite=external&targetcat=link&targettype=uri">CrossRef</a>]</div></dd><dt>7.</dt><dd><div class="bk_ref" id="g145-humanwholeserum.REF.7">Uematsu R, Furukawa J, Nakagawa H, Shinohara Y, Deguchi K, Monde K, Nishimura SI. High throughput quantitative glycomics and glycoform-focused proteomics of murine dermis and epidermis. <span><span class="ref-journal">Mol Cell Proteomics. </span>2005 Dec;<span class="ref-vol">4</span>(12):1977–89.</span> [<a href="https://pubmed.ncbi.nlm.nih.gov/16170054" ref="pagearea=cite-ref&targetsite=entrez&targetcat=link&targettype=pubmed">PubMed<span class="bk_prnt">: 16170054</span></a>] [<a href="http://dx.crossref.org/10.1074/mcp.M500203-MCP200" ref="pagearea=cite-ref&targetsite=external&targetcat=link&targettype=uri">CrossRef</a>]</div></dd></dl></div><h2 id="NBK593871_footnotes">Footnotes</h2><dl class="temp-labeled-list small"><dt></dt><dd><div id="g145-humanwholeserum.FN1"><p class="no_top_margin">The authors declare no competing or financial interests.</p></div></dd></dl><div class="bk_prnt_sctn"><h2>Figures</h2><div class="whole_rhythm bk_prnt_obj bk_first_prnt_obj"><div id="g145-humanwholeserum.F1" class="figure bk_fig"><div class="graphic"><img src="/books/NBK593871/bin/g145-humanwholeserum-Image001.jpg" alt="Figure 1: . Streamlined protocol for comprehensive GSL-glycomic analysis of human serum/plasma." /></div><h3><span class="label">Figure 1: </span></h3><div class="caption"><p>Streamlined protocol for comprehensive GSL-glycomic analysis of human serum/plasma. Reprinted and modified with permission from (<a class="bk_pop" href="#g145-humanwholeserum.REF.6">6</a>).</p></div></div></div><div class="whole_rhythm bk_prnt_obj"><div id="g145-humanwholeserum.F2" class="figure bk_fig"><div class="graphic"><a href="/core/lw/2.0/html/tileshop_pmc/tileshop_pmc_inline.html?title=Figure%202%3A%20.%20Quantitative%20GSL-glycan%20analysis%20of%20typical%20healthy%20human%20serum%20of%20blood%20groups%20A%2C%20B%2C%20O%2C%20and%20AB.&p=BOOKS&id=593871_g145-humanwholeserum-Image002.jpg" target="tileshopwindow" class="inline_block pmc_inline_block ts_canvas img_link" title="Click on image to zoom"><div class="ts_bar small" title="Click on image to zoom"></div><img src="/books/NBK593871/bin/g145-humanwholeserum-Image002.jpg" alt="Figure 2: . Quantitative GSL-glycan analysis of typical healthy human serum of blood groups A, B, O, and AB." class="tileshop" title="Click on image to zoom" /></a></div><h3><span class="label">Figure 2: </span></h3><div class="caption"><p>Quantitative GSL-glycan analysis of typical healthy human serum of blood groups A, B, O, and AB. The size of each circle and its constituent colors reflect the absolute quantity of glycans and the glycan substructures, respectively. Reprinted and modified with permission from (<a class="bk_pop" href="#g145-humanwholeserum.REF.6">6</a>).</p></div></div></div></div><div class="bk_prnt_sctn"><h2>Tables</h2><div class="whole_rhythm bk_prnt_obj bk_first_prnt_obj"><div id="g145-humanwholeserum.T.list_of_gslglycan" class="table"><h3><span class="label">Table 1: </span></h3><div class="caption"><p>List of GSL-glycans in human serum. Reprinted and modified with permission from (<a class="bk_pop" href="#g145-humanwholeserum.REF.6">6</a>).</p></div><p class="large-table-link" style="display:none"><span class="right"><a href="/books/NBK593871/table/g145-humanwholeserum.T.list_of_gslglycan/?report=objectonly" target="object">View in own window</a></span></p><div class="large_tbl" id="__g145-humanwholeserum.T.list_of_gslglycan_lrgtbl__"><table><thead><tr><th id="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="col" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">No.</th><th id="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" scope="col" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Class</th><th id="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" scope="col" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Glycan composition</th><th id="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" scope="col" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">m/z</th></tr></thead><tbody><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">LacCer</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">772.336</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2(Neu5Ac)1-lactone1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1045.425</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-3</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1077.452</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-4</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2(Neu5Ac)2 -lactone1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1350.541</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-5</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2(Neu5Ac)2 -Ac1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1424.577</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-6</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)2(Fuc)1(NeuAc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1791.721</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-7</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2(HexNAc)1(Neu5Ac)3-lactone2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1826.709</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-8</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gb</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">934.389</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-9</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1299.521</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-10</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Fuc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1429.584</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-11</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)2(Fuc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1486.606</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-12</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)1(Fuc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1591.637</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-13</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)2(Fuc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1632.664</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-14</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)5(HexNAc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1664.654</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-15</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)2(Fuc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1794.717</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-16</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)5(HexNAc)3</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1867.733</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-17</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)3(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">2010.795</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-18</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)5(HexNAc)3(Fuc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">2013.791</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-19</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)5(HexNAc)3(Fuc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">2159.849</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-20</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)5(HexNAc)3(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">2172.848</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-21</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)5(HexNAc)3(Fuc)1(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">2318.906</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-22</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2(HexNAc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">975.416</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-23</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)2(HexNAc)1(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1280.531</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-24</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Fuc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1283.527</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-25</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1442.584</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-26</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)1(Fuc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1445.579</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-27</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Neu5Ac)1-Ac1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1484.594</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-28</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1502.601</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-29</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Fuc)1(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1588.642</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-30</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)2(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1645.663</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-31</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)2(Fuc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1648.659</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-32</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Neu5Ac)2-lactone1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1715.673</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-33</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Neu5Ac)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1747.699</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-34</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)2(NeuAc)1-lactone1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1775.690</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-35</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(NeuAc)2-Ac1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1789.710</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-36</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)2(Neu5Ac)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1807.716</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-37</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(Neu5Ac)3-lactone1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">2020.788</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-38</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gb/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)3</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1705.680</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-39</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gb/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)5(HexNAc)2(Fuc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1810.712</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-40</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gb/(n)Lc</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)4(HexNAc)3(Fuc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1851.738</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-41</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc/Gb</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1137.469</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-42</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc/Gb</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)2</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1340.548</td></tr><tr><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_1" scope="row" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">GSL-43</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_2" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">Gg/(n)Lc/Gb</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_3" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">(Hex)3(HexNAc)1(NeuAc)1-lactone1</td><td headers="hd_h_g145-humanwholeserum.T.list_of_gslglycan_1_1_1_4" rowspan="1" colspan="1" style="text-align:left;vertical-align:top;">1410.558</td></tr></tbody></table></div></div></div></div><div id="bk_toc_contnr"></div></div></div>
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