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<div class="pre-content"><div><div class="bk_prnt"><p class="small">NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.</p></div><div class="pagination bk_noprnt"><span class="inactive page_link prev">< Prev</span><a class="active page_link next" href="/books/n/glycopodv2/editorialboard/" title="Next page in this title">Next ></a></div></div></div>
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<div class="main-content lit-style" itemscope="itemscope" itemtype="http://schema.org/Book"><div class="meta-content fm-sec"><div class="iconblock whole_rhythm clearfix no_top_margin"><a href="http://jcgg.jp/" title="Japan Consortium for Glycobiology and Glycotechnology" class="img_link icnblk_img" ref="pagearea=logo&targetsite=external&targetcat=link&targettype=publisher"><img class="source-thumb" src="/corehtml/pmc/pmcgifs/bookshelf/thumbs/th-glycopodv2-lrg.png" alt="Cover of Glycoscience Protocols (GlycoPODv2)" /></a><div class="icnblk_cntnt"><h1 id="_NBK593839_"><span itemprop="name">Glycoscience Protocols (GlycoPODv2)</span></h1><p class="contrib-group">Editors: <span itemprop="editor">Shoko Nishihara</span>, <span itemprop="editor">Kiyohiko Angata</span>, <span itemprop="editor">Kiyoko F. Aoki-Kinoshita</span>, and <span itemprop="editor">Jun Hirabayashi</span>.</p><div class="half_rhythm">Saitama (JP): <a href="http://jcgg.jp/" ref="pagearea=meta&targetsite=external&targetcat=link&targettype=publisher"><span itemprop="publisher">Japan Consortium for Glycobiology and Glycotechnology</span></a>; <span itemprop="datePublished">2021</span>-.</div><div class="half_rhythm"><ul class="inline_list"><li><span class="label"><a data-jig="ncbidialog" href="#_ncbi_dlg_cpyrght_NBK593839" data-jigconfig="modal:true">Copyright and
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To view a copy of this license, visit <a href="http://creativecommons.org/licenses/by-nc-nd/4.0/" ref="pagearea=meta&targetsite=external&targetcat=link&targettype=uri">http://creativecommons.org/licenses/by-nc-nd/4.0/</a>.</div></div></div></div><div class="bk_noprnt"><form method="get" action="/books/n/glycopodv2/" id="bk_srch"><div class="bk_search"><label for="bk_term" class="offscreen_noflow">Search term</label><input type="text" title="Search this book" id="bk_term" name="term" value="" data-jig="ncbiclearbutton" /> <input type="submit" class="jig-ncbibutton" value="Search this book" submit="false" style="padding: 0.1em 0.4em;" /></div></form></div></div></div></div><div class="body-content whole_rhythm" itemprop="text"><div><h2>Contents</h2><div class="bktoc_all_cntnr top align_right" style="display:none"><ul class="inline_list_right"><li><a class="bktoc_all_exp" href="#">Expand All</a></li><li style="margin-left:.8em"><a class="bktoc_all_clps" href="#">Collapse All</a></li></ul></div><ul id="toc_tllNBK593839_toc_editorialboard" class="simple-list toc toc-toggle"><li class="half_rhythm" id="toc_itm_NBK593839_toc_editorialboard"><a class="toc-item" href="/books/n/glycopodv2/editorialboard/">GlycoPOD v2 Editorial Board</a></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_preface"><a class="toc-item" href="/books/n/glycopodv2/preface/">Preface</a></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_purification"><a class="toc-item" href="/books/n/glycopodv2/purification/">Purification and separation</a><ul id="toc_lst_NBK593839_toc_purification" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_glycoproteins"><a class="toc-item" href="/books/n/glycopodv2/glycoproteins/">Glycoproteins</a><ul id="toc_lst_NBK593839_toc_glycoproteins" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g1-purificationanal"><a class="toc-item" href="/books/n/glycopodv2/g1-purificationanal/">Purification and analysis of free <i>N</i>-glycans in <i>Saccharomyces cerevisiae</i></a><div>Hiroto Hirayama and Tadashi Suzuki.</div><div class="small">Created: September 15, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g2-synthesisglyco"><a class="toc-item" href="/books/n/glycopodv2/g2-synthesisglyco/">Synthesis of glycoproteins <i>in vitro</i></a><div>Kodai Machida and Hiroaki Imataka.</div><div class="small">Created: September 27, 2021; Last Revision: March 15, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g3-releaseoxi"><a class="toc-item" href="/books/n/glycopodv2/g3-releaseoxi/">Release of <i>O</i>-glycan by eliminative oximation</a><div>Akihiko Kameyama.</div><div class="small">Created: October 1, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g4-prepglyco"><a class="toc-item" href="/books/n/glycopodv2/g4-prepglyco/">Preparation of <i>O</i>-glycopeptides by GlycOCATCH</a><div>Daisuke Takakura.</div><div class="small">Created: September 15, 2021; Last Revision: March 14, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g5-prephydro"><a class="toc-item" href="/books/n/glycopodv2/g5-prephydro/">Preparation of glycopeptides by hydrophilic interaction chromatography (HILIC)</a><div>Hiroyuki Kaji.</div><div class="small">Created: October 1, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g6-prepchroma"><a class="toc-item" href="/books/n/glycopodv2/g6-prepchroma/">Preparation of glycopeptides by lectin affinity chromatography (LAC)</a><div>Hiroyuki Kaji.</div><div class="small">Created: October 1, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g7-prepacetone"><a class="toc-item" href="/books/n/glycopodv2/g7-prepacetone/">Preparation of glycopeptides by acetone precipitation</a><div>Daisuke Takakura.</div><div class="small">Created: September 15, 2021; Last Revision: March 14, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_glycosaminoglycans"><a class="toc-item" href="/books/n/glycopodv2/glycosaminoglycans/">Glycosaminoglycans</a><ul id="toc_lst_NBK593839_toc_glycosaminoglycans" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g8-prepglycosam"><a class="toc-item" href="/books/n/glycopodv2/g8-prepglycosam/">Preparation of glycosaminoglycans</a><div>Shuji Mizumoto and Shuhei Yamada.</div><div class="small">Created: October 6, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g9-prepproteo"><a class="toc-item" href="/books/n/glycopodv2/g9-prepproteo/">Preparation of proteoglycans from tissues</a><div>Hideto Watanabe.</div><div class="small">Created: October 2, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g10-prepoligo"><a class="toc-item" href="/books/n/glycopodv2/g10-prepoligo/">Preparation of oligosaccharides from chondroitin sulfate with hyaluronidase</a><div>Shuji Mizumoto.</div><div class="small">Created: October 16, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g11-prepheparin"><a class="toc-item" href="/books/n/glycopodv2/g11-prepheparin/">Preparation of heparin/heparan sulfate oligosaccharides</a><div>Shuhei Yamada.</div><div class="small">Created: October 6, 2021; Last Revision: March 30, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_glycolipids"><a class="toc-item" href="/books/n/glycopodv2/glycolipids/">Glycolipids</a><ul id="toc_lst_NBK593839_toc_glycolipids" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g12-extractglyco"><a class="toc-item" href="/books/n/glycopodv2/g12-extractglyco/">Extraction of glycolipids</a><div>Nozomu Okino and Makoto Ito.</div><div class="small">Created: September 28, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g13-prepmembrane"><a class="toc-item" href="/books/n/glycopodv2/g13-prepmembrane/">Preparation of membrane rafts</a><div>Jin-ichi Inokuchi.</div><div class="small">Created: October 11, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g14-thinchroma"><a class="toc-item" href="/books/n/glycopodv2/g14-thinchroma/">Thin-layer chromatography (TLC) of glycolipids</a><div>Nozomu Okino and Makoto Ito.</div><div class="small">Created: September 29, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g15-tlcblot"><a class="toc-item" href="/books/n/glycopodv2/g15-tlcblot/">TLC-blot (far-eastern blot) and its application to MS analysis of glycosphingolipids</a><div>Naoko Goto-Inoue and Takao Taki.</div><div class="small">Created: September 1, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g16-prepdolichyl"><a class="toc-item" href="/books/n/glycopodv2/g16-prepdolichyl/">Preparation of dolichylpyrophosphoryl oligosaccharides from <i>Saccharomyces cerevisiae</i></a><div>Yoichiro Harada.</div><div class="small">Created: October 4, 2021; Last Revision: March 22, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_oligosaccharides"><a class="toc-item" href="/books/n/glycopodv2/oligosaccharides/">Oligosaccharides</a><ul id="toc_lst_NBK593839_toc_oligosaccharides" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g17-largeoligo"><a class="toc-item" href="/books/n/glycopodv2/g17-largeoligo/">Large scale preparation of oligosaccharides from chicken eggs</a><div>Yasuhiro Kajihara.</div><div class="small">Created: November 11, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g18-prephumanmilk"><a class="toc-item" href="/books/n/glycopodv2/g18-prephumanmilk/">Preparation of oligosaccharides from human milk</a><div>Junko Amano.</div><div class="small">Created: October 4, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g19-methodglycans"><a class="toc-item" href="/books/n/glycopodv2/g19-methodglycans/">Purification method for serum free glycans</a><div>Chengcheng Huang and Tadashi Suzuki.</div><div class="small">Created: November 2, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g20-prepmammal"><a class="toc-item" href="/books/n/glycopodv2/g20-prepmammal/">Preparation of neutral free oligosaccharides from mammalian cultured cells</a><div>Yoichiro Harada.</div><div class="small">Created: October 4, 2021; Last Revision: March 22, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_lectins"><a class="toc-item" href="/books/n/glycopodv2/lectins/">Lectins</a><ul id="toc_lst_NBK593839_toc_lectins" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g21-prodgalectin1"><a class="toc-item" href="/books/n/glycopodv2/g21-prodgalectin1/">Production, purification and quality control of galectin-1</a><div>Guillaume St-Pierre and Sachiko Sato.</div><div class="small">Created: October 23, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g22-prodgalectin3"><a class="toc-item" href="/books/n/glycopodv2/g22-prodgalectin3/">Production, purification and quality control of galectin-3</a><div>Guillaume St-Pierre and Sachiko Sato.</div><div class="small">Created: October 23, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g23-preprecomb"><a class="toc-item" href="/books/n/glycopodv2/g23-preprecomb/">Preparation of recombinant galectin protein: expression, affinity purification, and removal of lipopolysaccharide</a><div>Tomoharu Takeuchi.</div><div class="small">Created: October 1, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g24-isohepasialo"><a class="toc-item" href="/books/n/glycopodv2/g24-isohepasialo/">Isolation and determination of hepatic asialoglycoprotein receptor</a><div>Toshisuke Kawasaki and Hiromi Nakao.</div><div class="small">Created: October 23, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g25-isoprotein"><a class="toc-item" href="/books/n/glycopodv2/g25-isoprotein/">Isolation of mannan-binding protein from human serum (plasma)</a><div>Katsuaki Higashi, Motohiro Nonaka, Nobuko Kawasaki, and Toshisuke Kawasaki.</div><div class="small">Created: September 1, 2021; Last Revision: May 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g26-isopulmonary"><a class="toc-item" href="/books/n/glycopodv2/g26-isopulmonary/">Isolation of pulmonary surfactant proteins from bronchoalveolar lavage fluids</a><div>Shigeru Ariki, Chiaki Nishitani, and Yoshio Kuroki.</div><div class="small">Created: September 30, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g27-purificolin2"><a class="toc-item" href="/books/n/glycopodv2/g27-purificolin2/">Purification and binding assay of human ficolin-2</a><div>Misao Matsushita.</div><div class="small">Created: September 30, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g28-isobindingassay"><a class="toc-item" href="/books/n/glycopodv2/g28-isobindingassay/">Isolation and binding assay of ER degradation-enhancing α-mannosidase-like proteins (EDEMs)</a><div>Nobuko Hosokawa.</div><div class="small">Created: September 21, 2021; Last Revision: March 25, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_others"><a class="toc-item" href="/books/n/glycopodv2/others/">Others</a><ul id="toc_lst_NBK593839_toc_others" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g29-bluenative"><a class="toc-item" href="/books/n/glycopodv2/g29-bluenative/">Blue native polyacrylamide gel electrophoresis</a><div>Yoichiro Harada.</div><div class="small">Created: October 4, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g30-prepmouseliver"><a class="toc-item" href="/books/n/glycopodv2/g30-prepmouseliver/">Preparation of mouse liver microsome</a><div>Yoshimi Haga.</div><div class="small">Created: July 28, 2021; Last Revision: March 16, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g31-enrichsulfated"><a class="toc-item" href="/books/n/glycopodv2/g31-enrichsulfated/">Enrichment of sulfated glycopeptides by sulfate emerging method</a><div>Akihiko Kameyama and Masaaki Toyoda.</div><div class="small">Created: October 1, 2021; Last Revision: March 24, 2022.</div></li></ul></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_activity"><a class="toc-item" href="/books/n/glycopodv2/activity/">Activity and functional analysis</a><ul id="toc_lst_NBK593839_toc_activity" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_enzymes"><a class="toc-item" href="/books/n/glycopodv2/enzymes/">Enzymes: Hydrolases and lyases</a><ul id="toc_lst_NBK593839_toc_enzymes" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g32-endogalact"><a class="toc-item" href="/books/n/glycopodv2/g32-endogalact/">Endo-β-galactosidase digestion</a><div>Michiko Fukuda, N and Tomoya Akama, O.</div><div class="small">Created: September 30, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g33-enzymeassayendob"><a class="toc-item" href="/books/n/glycopodv2/g33-enzymeassayendob/">Enzyme assay of glycoproteins by endo-β-N-acetylglucosaminidases</a><div>David Martin C. de los Reyes, Kristina Mae Bienes, and Kaoru Takegawa.</div><div class="small">Created: September 14, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g34-chemprobeendo"><a class="toc-item" href="/books/n/glycopodv2/g34-chemprobeendo/">A chemical probe for the detection of endo-β-<i>N</i>-acetylglucosaminidase activity</a><div>Nozomi Ishii and Ichiro Matsuo.</div><div class="small">Created: December 23, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g35-enzymeassayacetyl"><a class="toc-item" href="/books/n/glycopodv2/g35-enzymeassayacetyl/">Enzyme assay for β-<i>N</i>-acetylhexosaminidase</a><div>Hisashi Ashida and Kenji Yamamoto.</div><div class="small">Created: August 31, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g36-assaysialidase"><a class="toc-item" href="/books/n/glycopodv2/g36-assaysialidase/">Enzyme assay for mammalian sialidases</a><div>Taeko Miyagi, Koji Yamamoto, and Kazuhiro Shiozaki.</div><div class="small">Created: October 7, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g37-enzymeassayendo"><a class="toc-item" href="/books/n/glycopodv2/g37-enzymeassayendo/">Enzyme assay of endogenous activity of peptide-<i>N</i>-glycanase (PNGase)/<i>N</i>-Glycanase 1 (NGLY1) in cells and tissues using glycosylated cyclopeptide as a substrate</a><div>Hiroto Hirayama and Tadashi Suzuki.</div><div class="small">Created: September 15, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g38-proteinassayendo"><a class="toc-item" href="/books/n/glycopodv2/g38-proteinassayendo/">Protein degradation assay for endoplasmic reticulum-associated degradation (ERAD) in mammalian cells</a><div>Nobuko Hosokawa.</div><div class="small">Created: September 21, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g39-assaynucleo"><a class="toc-item" href="/books/n/glycopodv2/g39-assaynucleo/">Enzymatic assay of nucleocytoplasmic <i>O</i>-linked β-<i>N</i>-acetylglucosaminidase (<i>O</i>-GlcNAcase)</a><div>Kazuo Kamemura.</div><div class="small">Created: September 23, 2021; Last Revision: March 16, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g40-detectenzyme"><a class="toc-item" href="/books/n/glycopodv2/g40-detectenzyme/">Detection of heparanase enzyme activity</a><div>Nobuaki Higashi.</div><div class="small">Created: November 12, 2021; Last Revision: March 26, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g41-heparandigest"><a class="toc-item" href="/books/n/glycopodv2/g41-heparandigest/">Heparan sulfate/heparin digesting enzymes from bacteria</a><div>Naoko Nagai.</div><div class="small">Created: October 1, 2021; Last Update: November 23, 2021.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g42-chondroitin"><a class="toc-item" href="/books/n/glycopodv2/g42-chondroitin/">Chondroitin sulfate/dermatan sulfate-digesting enzymes from bacteria</a><div>Takashi Kobayashi and Nobuo Sugiura.</div><div class="small">Created: August 19, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g43-keratan"><a class="toc-item" href="/books/n/glycopodv2/g43-keratan/">Keratan sulfate-degrading enzymes from bacteria</a><div>Kiwamu Yamagishi and Hideto Watanabe.</div><div class="small">Created: November 1, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g44-bacthyaluronan"><a class="toc-item" href="/books/n/glycopodv2/g44-bacthyaluronan/">Bacterial glycosaminoglycan lyases to depolymerize hyaluronan</a><div>Shuhei Yamada.</div><div class="small">Created: October 5, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g45-assaypyro"><a class="toc-item" href="/books/n/glycopodv2/g45-assaypyro/">Assay for dolichol-linked oligosaccharide: pyrophosphatase</a><div>Sheng-tao Li and Tadashi Suzuki.</div><div class="small">Created: August 30, 2021; Last Revision: March 15, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g46-degradationglyco"><a class="toc-item" href="/books/n/glycopodv2/g46-degradationglyco/">Degradation of glycolipids by endoglycoceramidase</a><div>Yohei Ishibashi and Makoto Ito.</div><div class="small">Created: September 29, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g47-hydroceramide"><a class="toc-item" href="/books/n/glycopodv2/g47-hydroceramide/">Hydrolysis of ceramide by ceramidase (CDase) and measurement of CDase activity</a><div>Nozomu Okino and Makoto Ito.</div><div class="small">Created: September 28, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g48-hydrosphingolipid"><a class="toc-item" href="/books/n/glycopodv2/g48-hydrosphingolipid/">Hydrolysis of glycosphingolipids by a sphingolipid ceramide <i>N</i>-deacylase (SCDase)</a><div>Nozomu Okino and Makoto Ito.</div><div class="small">Created: September 28, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g49-analsphingolipid"><a class="toc-item" href="/books/n/glycopodv2/g49-analsphingolipid/">Analysis of sphingolipid activator protein activity for sphingolipid hydrolases</a><div>Junko Matsuda, Kazuhito Tsuboi, and Azusa Yoneshige.</div><div class="small">Created: September 28, 2021; Last Revision: March 16, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_transferases"><a class="toc-item" href="/books/n/glycopodv2/transferases/">Enzymes: Transferases</a><ul id="toc_lst_NBK593839_toc_transferases" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g50-assaypoglut1"><a class="toc-item" href="/books/n/glycopodv2/g50-assaypoglut1/">Assay of protein <i>O</i>-glucosyltransferase 1 (POGLUT1) glycosyltransferase activity</a><div>Katsuki Sato, Tadanobu Takahashi, and Hideyuki Takeuchi.</div><div class="small">Created: October 15, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g51-assaya4galt"><a class="toc-item" href="/books/n/glycopodv2/g51-assaya4galt/">Enzyme assay of glycolipid glycosyltransferases: Gb3/CD77 synthase (A4GALT)</a><div>Koichi Furukawa, Keiko Furukawa, Robiul Bhuiyan, and Yuji Kondo.</div><div class="small">Created: January 8, 2022; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g52-assayyeast"><a class="toc-item" href="/books/n/glycopodv2/g52-assayyeast/">Enzyme assay of yeast glycosyltransferases: α1,2-galactosyltransferase</a><div>Takehiko Yoko-o.</div><div class="small">Created: December 1, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g53-generalmethods"><a class="toc-item" href="/books/n/glycopodv2/g53-generalmethods/">General methods for detection of enzyme reaction products of polypeptide <i>N</i>-acetylgalactosaminyltransferase, β1,3-glycosyltransferase, and β1,4-glycosyltransferases</a><div>Akira Togayachi, Takashi Sato, Tomomi Kubota, and Hisashi Narimatsu.</div><div class="small">Created: December 20, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g54-galtassay"><a class="toc-item" href="/books/n/glycopodv2/g54-galtassay/">Enzyme assay of β1,3-glycosyltransferase family</a><div>Akira Togayachi.</div><div class="small">Created: December 20, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g55-assaypomt"><a class="toc-item" href="/books/n/glycopodv2/g55-assaypomt/">Enzyme assay of protein <i>O</i>-mannosyltransferase (POMT1/2)</a><div>Keiko Akasaka-Manya, Hiroshi Manya, and Tamao Endo.</div><div class="small">Created: October 27, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g56-assaygnt3mgat3"><a class="toc-item" href="/books/n/glycopodv2/g56-assaygnt3mgat3/">Enzyme assay of <i>N</i>-acetylglucosaminyltransferase-III (GnT-III, MGAT3)</a><div>Kento Maeda and Naoyuki Taniguchi.</div><div class="small">Created: October 1, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g57-assaygnt4mgat4"><a class="toc-item" href="/books/n/glycopodv2/g57-assaygnt4mgat4/">Enzyme assay of <i>N</i>-acetylglucosaminyltransferase-IV (GnT-IVa,b, MGAT4A,B)</a><div>Kento Maeda and Naoyuki Taniguchi.</div><div class="small">Created: October 1, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g58-assaygnt4cmgat4c"><a class="toc-item" href="/books/n/glycopodv2/g58-assaygnt4cmgat4c/">Enzyme assay of <i>N</i>-acetylglucosaminyltransferase VI (GnT-VI, GnT-IVc, MGAT4C)</a><div>Tomohiko Taguchi.</div><div class="small">Created: September 23, 2021; Last Revision: March 13, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g59-assaymgat5"><a class="toc-item" href="/books/n/glycopodv2/g59-assaymgat5/">Enzyme assay of <i>N</i>-acetylglucosaminyltransferase-V (GnT-V, MGAT5)</a><div>Kento Maeda and Naoyuki Taniguchi.</div><div class="small">Created: October 1, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g60-assaymgat5b"><a class="toc-item" href="/books/n/glycopodv2/g60-assaymgat5b/">Enzyme assay of <i>N</i>-acetylglucosaminyltransferase IX/Vb (MGAT5B)</a><div>Kei-ichiro Inamori, Kento Maeda, and Naoyuki Taniguchi.</div><div class="small">Created: October 1, 2021; Last Revision: March 15, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g61-assayfringe"><a class="toc-item" href="/books/n/glycopodv2/g61-assayfringe/">Enzyme assay of Fringe</a><div>Yuko Tashima.</div><div class="small">Created: November 15, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g62-assayeogt"><a class="toc-item" href="/books/n/glycopodv2/g62-assayeogt/">Enzyme assay of <i>O</i>-GlcNAc transferase</a><div>Sayad Md. Didarul Alam and Tetsuya Okajima.</div><div class="small">Created: August 14, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g63-assayogt"><a class="toc-item" href="/books/n/glycopodv2/g63-assayogt/">Enzymatic assay of nucleocytoplasmic <i>O</i>-linked β-<i>N</i>-acetylglucosaminyltransferase (<i>O</i>-GlcNAc transferase, OGT)</a><div>Kazuo Kamemura.</div><div class="small">Created: September 23, 2021; Last Revision: March 16, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g64-assaypomgnt"><a class="toc-item" href="/books/n/glycopodv2/g64-assaypomgnt/">Enzyme assay of protein <i>O</i>-linked-mannose β1,2-<i>N</i>-acetylglucosaminyltransferase 1 and protein <i>O</i>-linked-mannose β1,4-<i>N</i>-acetylglucosaminyltransferase 2 (POMGNT1/2)</a><div>Keiko Akasaka-Manya, Hiroshi Manya, and Tamao Endo.</div><div class="small">Created: January 25, 2022; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g65-assayppgalnact"><a class="toc-item" href="/books/n/glycopodv2/g65-assayppgalnact/">Enzyme assay of polypeptide <i>N</i>-acetylgalactosaminyltransferase family</a><div>Akira Togayachi and Hisashi Narimatsu.</div><div class="small">Created: December 20, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g66-assayb3galnt2"><a class="toc-item" href="/books/n/glycopodv2/g66-assayb3galnt2/">Enzyme assay of β1,3-<i>N</i>-acetylgalactosaminyltransferase 2 (B3GALNT2)</a><div>Kiyohiko Angata.</div><div class="small">Created: December 20, 2021; Last Revision: March 26, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g67-assayb4galnt1"><a class="toc-item" href="/books/n/glycopodv2/g67-assayb4galnt1/">Enzyme assay of glycolipid glycosyltransferases: GM2/GD2 synthase (β1,4-GalNAc transferase, B4GALNT1)</a><div>Koichi Furukawa, Keiko Furukawa, Yuhsuke Ohmi, and Robiul Bhuiyan.</div><div class="small">Created: January 8, 2022; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g68-assayfut3"><a class="toc-item" href="/books/n/glycopodv2/g68-assayfut3/">Enzyme assay of α1,3/4-fucosyltransferase</a><div>Akira Togayachi and Takashi Kudo.</div><div class="small">Created: December 20, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g69-assayfut8"><a class="toc-item" href="/books/n/glycopodv2/g69-assayfut8/">Enzyme assay of fucosyltransferase 8 (FUT8)</a><div>Hideyuki Ihara and Yoshitaka Ikeda.</div><div class="small">Created: October 1, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g70-assaydofut1"><a class="toc-item" href="/books/n/glycopodv2/g70-assaydofut1/">Enzyme assay of <i>O</i>-fucosyltransferase 1 using <i>Drosophila</i> OFUT1</a><div>Tetsuya Okajima.</div><div class="small">Created: November 13, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g71-assaysiatp"><a class="toc-item" href="/books/n/glycopodv2/g71-assaysiatp/">Enzyme assay of sialyltransferases for glycoproteins</a><div>Shou Takashima.</div><div class="small">Created: September 28, 2021; Last Revision: May 20, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g72-Eassaysiatoligo"><a class="toc-item" href="/books/n/glycopodv2/g72-Eassaysiatoligo/">Enzyme assay of sialyltransferases for oligosaccharides</a><div>Shou Takashima.</div><div class="small">Created: September 28, 2021; Last Revision: May 20, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g73-assayst3gal5"><a class="toc-item" href="/books/n/glycopodv2/g73-assayst3gal5/">Enzyme assay of GM3 synthase (lactosylceramide α2,3-sialyltransferase, GM3S, ST3GAL5)</a><div>Kei-ichiro Inamori and Jin-ichi Inokuchi.</div><div class="small">Created: November 11, 2021; Last Revision: May 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g74-assayst8sia1"><a class="toc-item" href="/books/n/glycopodv2/g74-assayst8sia1/">Enzyme assay of glycolipid glycosyltransferases: GD3 synthase (α2,8-sialyltransferase, ST8SIAI)</a><div>Koichi Furukawa, Keiko Furukawa, and Yuhsuke Ohmi.</div><div class="small">Created: January 8, 2022; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g75-assaypolysiat"><a class="toc-item" href="/books/n/glycopodv2/g75-assaypolysiat/">Assay for enzymatic activity of polysialyltransferase</a><div>Masaya Hane and Chihiro Sato.</div><div class="small">Created: November 1, 2021; Last Revision: April 27, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g76-assayglcatp"><a class="toc-item" href="/books/n/glycopodv2/g76-assayglcatp/">Enzyme assay of glucuronyltransferases for HNK-1 epitope (GlcAT-P, B3GAT1 and GlcAT-S, B3GAT2)</a><div>Yasuhiko Kizuka, Jyoji Morise, and Shogo Oka.</div><div class="small">Created: September 7, 2021; Last Revision: March 14, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g77-assaygaggtcs"><a class="toc-item" href="/books/n/glycopodv2/g77-assaygaggtcs/">Enzyme assay of glycosaminoglycan glycosyltransferases for chondroitin sulfate</a><div>Satomi Nadanaka and Hiroshi Kitagawa.</div><div class="small">Created: October 13, 2021; Last Revision: March 20, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g78-gaggths"><a class="toc-item" href="/books/n/glycopodv2/g78-gaggths/">Enzyme assay of glycosaminoglycan glycosyltransferases for heparan sulfate</a><div>Satomi Nadanaka and Hiroshi Kitagawa.</div><div class="small">Created: October 13, 2021; Last Revision: March 20, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g79-assayhas"><a class="toc-item" href="/books/n/glycopodv2/g79-assayhas/">Enzyme assay of hyaluronan synthase</a><div>Naoki Itano.</div><div class="small">Created: September 28, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g80-assaygaggtks"><a class="toc-item" href="/books/n/glycopodv2/g80-assaygaggtks/">Enzyme assay of glycosaminoglycan glycosyltransferases for keratan sulfate</a><div>Akira Seko.</div><div class="small">Created: September 7, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g81-assayxylosylt"><a class="toc-item" href="/books/n/glycopodv2/g81-assayxylosylt/">Enzyme assay of xylosyltransferase</a><div>Satomi Nadanaka and Hiroshi Kitagawa.</div><div class="small">Created: October 13, 2021; Last Revision: March 20, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g82-assaygxyltxxylt"><a class="toc-item" href="/books/n/glycopodv2/g82-assaygxyltxxylt/">Assay of glucoside α1,3-xylosyltransferase 1/2 (GXYLT1/2) and xyloside α1,3-xylosyltransferase 1 (XXYLT1) xylosyltransferase activity</a><div>Amika Yoshioka, Yuuki Kurebayashi, and Hideyuki Takeuchi.</div><div class="small">Created: October 15, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g83-assay6st"><a class="toc-item" href="/books/n/glycopodv2/g83-assay6st/">Enzyme assay of <i>N</i>-acetylglucosamine-6-<i>O</i>-sulfotransferases</a><div>Kenji Uchimura.</div><div class="small">Created: September 26, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g84-assaychst10"><a class="toc-item" href="/books/n/glycopodv2/g84-assaychst10/">Enzyme assay of sulfotransferase for HNK-1 epitope (HNK-1ST, CHST10)</a><div>Yasuhiko Kizuka, Jyoji Morise, and Shogo Oka.</div><div class="small">Created: September 7, 2021; Last Revision: March 14, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g218-enzasssulfocd"><a class="toc-item" href="/books/n/glycopodv2/g218-enzasssulfocd/">Enzyme assay of sulfotransferases for chondroitin/dermatan</a><div>Osami Habuchi.</div><div class="small">Created: October 1, 2021; Last Revision: February 9, 2023.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g219-enzasssucsds"><a class="toc-item" href="/books/n/glycopodv2/g219-enzasssucsds/">Enzyme assay of sulfotransferases for chondroitin sulfate/dermatan sulfate</a><div>Osami Habuchi.</div><div class="small">Created: October 1, 2021; Last Revision: February 9, 2023.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g220-enzasssulfohs"><a class="toc-item" href="/books/n/glycopodv2/g220-enzasssulfohs/">Enzyme assay of sulfotransferases for heparan sulfate</a><div>Hiroko Habuchi and Koji Kimata.</div><div class="small">Created: October 1, 2021; Last Revision: February 9, 2023.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g85-assaystks"><a class="toc-item" href="/books/n/glycopodv2/g85-assaystks/">Enzyme assay of sulfotransferases for keratan sulfate</a><div>Akira Seko.</div><div class="small">Created: September 7, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g86-assaycersulfot"><a class="toc-item" href="/books/n/glycopodv2/g86-assaycersulfot/">Enzyme assay of cerebroside sulfotransferase</a><div>Koichi Honke.</div><div class="small">Created: October 19, 2021; Last Revision: March 15, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g87-assaypomk"><a class="toc-item" href="/books/n/glycopodv2/g87-assaypomk/">Enzyme assay of protein <i>O</i>-mannose kinase (POMK)</a><div>Hiroshi Manya and Tamao Endo.</div><div class="small">Created: January 25, 2022; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g88-assayfktnfkrp"><a class="toc-item" href="/books/n/glycopodv2/g88-assayfktnfkrp/">Enzyme assay of fukutin and fukutin-related protein (ribitol phosphate transferase, FKTN and FKRP)</a><div>Rieko Imae, Hiroshi Manya, and Tamao Endo.</div><div class="small">Created: November 10, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g89-assayrxylt"><a class="toc-item" href="/books/n/glycopodv2/g89-assayrxylt/">Enzyme assay of ribitol xylosyltransferase 1 (RXYLT1)</a><div>Shunsuke Hoshino, Hiroshi Manya, and Tamao Endo.</div><div class="small">Created: November 10, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g90-assaylarge"><a class="toc-item" href="/books/n/glycopodv2/g90-assaylarge/">Enzyme assay of protein-<i>O</i>-mannosyl glycan glycosyltransferases (LARGE1/2)</a><div>Kei-ichiro Inamori.</div><div class="small">Created: October 1, 2021; Last Revision: March 15, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_otherenzymes"><a class="toc-item" href="/books/n/glycopodv2/otherenzymes/">Enzymes: Others</a><ul id="toc_lst_NBK593839_toc_otherenzymes" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g91-altsabyhyd"><a class="toc-item" href="/books/n/glycopodv2/g91-altsabyhyd/">Alteration of cellular sialic acid composition by transfection of cytidine monophospho-<i>N</i>-acetylneuraminic acid hydroxylase</a><div>Yuko Naito-Matsui and Hiromu Takematsu.</div><div class="small">Created: September 24, 2021; Last Revision: March 15, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g92-assaycmah"><a class="toc-item" href="/books/n/glycopodv2/g92-assaycmah/">Enzyme assay for CMP-Neu5Ac hydroxylase (CMAH)</a><div>Hiromu Takematsu.</div><div class="small">Created: August 16, 2021; Last Revision: March 16, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g93-assaysps"><a class="toc-item" href="/books/n/glycopodv2/g93-assaysps/">Enzyme assay of sialic acid 9-phosphate synthase (SPS)</a><div>Di Wu and Ken Kitajima.</div><div class="small">Created: January 20, 2022; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g94-assayspl"><a class="toc-item" href="/books/n/glycopodv2/g94-assayspl/">Enzyme assay of sialate-pyruvate lyase (SPL)</a><div>Di Wu, Takahiro Nakagawa, and Ken Kitajima.</div><div class="small">Created: January 19, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g95-assaycmas"><a class="toc-item" href="/books/n/glycopodv2/g95-assaycmas/">Enzyme assay of cytidine 5’-monophosphate sialic acid synthetase (CMAS)</a><div>Di Wu and Ken Kitajima.</div><div class="small">Created: January 19, 2022; Last Revision: March 30, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_lectins2"><a class="toc-item" href="/books/n/glycopodv2/lectins2/">Lectins</a><ul id="toc_lst_NBK593839_toc_lectins2" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g96-assaygalectin9"><a class="toc-item" href="/books/n/glycopodv2/g96-assaygalectin9/">Assay method for the antiproliferative activity of human galectin-9</a><div>Aiko Itoh and Nozomu Nishi.</div><div class="small">Created: July 29, 2021; Last Revision: March 16, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g97-expgalectin9"><a class="toc-item" href="/books/n/glycopodv2/g97-expgalectin9/">Expression and purification of recombinant human galectin-9</a><div>Aiko Itoh and Nozomu Nishi.</div><div class="small">Created: July 29, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g98-recmanbinpro"><a class="toc-item" href="/books/n/glycopodv2/g98-recmanbinpro/">Recombinant protein preparation and functional analysis of mannan-binding protein</a><div>Motohiro Nonaka and Toshisuke Kawasaki.</div><div class="small">Created: October 1, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g99-mannanbinding"><a class="toc-item" href="/books/n/glycopodv2/g99-mannanbinding/">Assay method for the lectin activity of mannan-binding protein</a><div>Katsuaki Higashi, Motohiro Nonaka, Nobuko Kawasaki, and Toshisuke Kawasaki.</div><div class="small">Created: September 1, 2021; Last Revision: April 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g100-selecbindtu"><a class="toc-item" href="/books/n/glycopodv2/g100-selecbindtu/">Selectin-binding analysis of tumor cells</a><div>Keiichiro Sakuma and Reiji Kannagi.</div><div class="small">Created: September 6, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g101-SWassay"><a class="toc-item" href="/books/n/glycopodv2/g101-SWassay/">Modified Stamper-Woodruff cell-binding assay</a><div>Hiroro Kawashima.</div><div class="small">Created: January 20, 2022; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g102-lymphohoming"><a class="toc-item" href="/books/n/glycopodv2/g102-lymphohoming/">Assay of glycan-mediated lymphocyte homing</a><div>Hiroto Kawashima.</div><div class="small">Created: January 20, 2022; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g103-MGLflowcyto"><a class="toc-item" href="/books/n/glycopodv2/g103-MGLflowcyto/">Flow cytometric analysis of macrophage galactose-type C-type lectin 1 and 2 expression by the use of specific monoclonal antibodies</a><div>Kaori Denda-Nagai, Ryuichi Murakami, and Tatsuro Irimura.</div><div class="small">Created: February 7, 2022; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g104-calreticulinITC"><a class="toc-item" href="/books/n/glycopodv2/g104-calreticulinITC/">Binding assay of calreticulin using isothermal titration calorimetry</a><div>Ichiro Matsuo and Yoichi Takeda.</div><div class="small">Created: October 4, 2021; Last Revision: March 27, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g105-bindingERGIC53"><a class="toc-item" href="/books/n/glycopodv2/g105-bindingERGIC53/">Expression and binding assay of endoplasmic reticulum-Golgi intermediate compartment protein 53 (ERGIC-53, LMAN1)</a><div>Kazuo Yamamoto.</div><div class="small">Created: September 29, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g106-bindingVIP36"><a class="toc-item" href="/books/n/glycopodv2/g106-bindingVIP36/">Expression and binding assay of vesicular integral membrane protein 36 (VIP36, LMAN2)</a><div>Kazuo Yamamoto.</div><div class="small">Created: September 29, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g107-lectinreporter"><a class="toc-item" href="/books/n/glycopodv2/g107-lectinreporter/">Analysis of intracellular lectins using reporter cell expressing lectin on its surface</a><div>Kazuo Yamamoto.</div><div class="small">Created: September 29, 2021; Last Revision: April 5, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g108-Siglec7function"><a class="toc-item" href="/books/n/glycopodv2/g108-Siglec7function/">Recombinant sugar binding proteins and their functional analysis: Siglec-7</a><div>Toshiyuki Yamaji and Yasuhiro Hashimoto.</div><div class="small">Created: September 17, 2021; Last Revision: March 14, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g109-HAassay"><a class="toc-item" href="/books/n/glycopodv2/g109-HAassay/">Hemagglutination assay for the estimation of a carbohydrate-binding activity and determination of its carbohydrate-binding specificity</a><div>Guillaume St-Pierre and Sachiko Sato.</div><div class="small">Created: October 17, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g110-ELISAinfluenza"><a class="toc-item" href="/books/n/glycopodv2/g110-ELISAinfluenza/">Enzyme-linked immunosorbent assay (ELISA)-based method for determination of influenza virus-host receptor binding specificity</a><div>Yasuo Suzuki and Nongluk Sriwilaijaroen.</div><div class="small">Created: November 24, 2021; Last Revision: March 23, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_transporters"><a class="toc-item" href="/books/n/glycopodv2/transporters/">Transporters</a><ul id="toc_lst_NBK593839_toc_transporters" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g111-sugartransport"><a class="toc-item" href="/books/n/glycopodv2/g111-sugartransport/">Assay of nucleotide sugar transport activity (Golgi/ER transporter)</a><div>Shoko Nishihara and Ken-ichi Nakayama.</div><div class="small">Created: August 16, 2021; Last Revision: April 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g112-PAPStransport"><a class="toc-item" href="/books/n/glycopodv2/g112-PAPStransport/">Assay of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transport activity</a><div>Shoko Nishihara.</div><div class="small">Created: August 11, 2021; Last Revision: April 30, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_others2"><a class="toc-item" href="/books/n/glycopodv2/others2/">Others</a><ul id="toc_lst_NBK593839_toc_others2" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g113-iletglucose"><a class="toc-item" href="/books/n/glycopodv2/g113-iletglucose/">Analyses of glucose sensor function of pancreatic islet cells</a><div>Kazuaki Ohtsubo.</div><div class="small">Created: August 31, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g114-EMARS"><a class="toc-item" href="/books/n/glycopodv2/g114-EMARS/">Enzyme-mediated activation of radical sources (EMARS)-based molecular interaction analysis</a><div>Norihiro Kotani, Arisa Yamaguchi, and Koichi Honke.</div><div class="small">Created: August 11, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g115-FRET"><a class="toc-item" href="/books/n/glycopodv2/g115-FRET/">Imaging of glycoforms by transmembrane Förster resonance energy transfer (FRET)</a><div>Yoshimi Haga.</div><div class="small">Created: August 3, 2021; Last Update: September 6, 2021.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g116-Mucin1signaling"><a class="toc-item" href="/books/n/glycopodv2/g116-Mucin1signaling/">Functional analyses of Mucin-1-mediated signaling and subsequent tumor progression</a><div>Shungo Iwamoto, Naoki Itano, and Hiroshi Nakada.</div><div class="small">Created: September 25, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g117-growthheparin"><a class="toc-item" href="/books/n/glycopodv2/g117-growthheparin/">Regulation of growth factor activity by heparin/heparan sulfate proteoglycan</a><div>Satomi Nadanaka and Hiroshi Kitagawa.</div><div class="small">Created: October 26, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g118-cellceramide"><a class="toc-item" href="/books/n/glycopodv2/g118-cellceramide/">Cell death/survival signal by ceramide and sphingosine-1-phosphate</a><div>Kazuyuki Kitatani, Makoto Taniguchi, and Toshiro Okazaki.</div><div class="small">Created: October 12, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g119-glucoceramide"><a class="toc-item" href="/books/n/glycopodv2/g119-glucoceramide/">Inhibitors of glucosylceramide synthase</a><div>Jin-ichi Inokuchi.</div><div class="small">Created: September 22, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g120-colitis"><a class="toc-item" href="/books/n/glycopodv2/g120-colitis/">Induction of colitis and its analyses</a><div>Shinichiro Shinzaki and Eiji Miyoshi.</div><div class="small">Created: September 2, 2021; Last Revision: April 5, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g121-ERADrate"><a class="toc-item" href="/books/n/glycopodv2/g121-ERADrate/">Rate of ER-associated degradation (ERAD) of misfolded proteins in <i>Saccharomyces cerevisiae</i></a><div>Akira Hosomi.</div><div class="small">Created: October 1, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g122-genomeediting"><a class="toc-item" href="/books/n/glycopodv2/g122-genomeediting/">Strategies of genome editing design for gene knockout</a><div>Seiya Mizuno and Hayate Suzuki.</div><div class="small">Created: September 29, 2021; Last Revision: March 14, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g123-tetraploidresc"><a class="toc-item" href="/books/n/glycopodv2/g123-tetraploidresc/">Tetraploid rescue experiment</a><div>Masahide Asano.</div><div class="small">Created: October 23, 2021; Last Revision: March 30, 2022.</div></li></ul></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_glycancharacter"><a class="toc-item" href="/books/n/glycopodv2/glycancharacter/">Glycan characterization</a><ul id="toc_lst_NBK593839_toc_glycancharacter" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_sample"><a class="toc-item" href="/books/n/glycopodv2/sample/">Sample preparation for characterization</a><ul id="toc_lst_NBK593839_toc_sample" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g124-radioactivesug"><a class="toc-item" href="/books/n/glycopodv2/g124-radioactivesug/">Metabolic labeling of glycans by radioactive sugars</a><div>Takashi Ohkura.</div><div class="small">Created: September 28, 2021; Last Revision: February 3, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g125-permethylation"><a class="toc-item" href="/books/n/glycopodv2/g125-permethylation/">Permethylation for glycan analysis</a><div>Akihiko Kameyama.</div><div class="small">Created: October 1, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g126-glycoproteins"><a class="toc-item" href="/books/n/glycopodv2/g126-glycoproteins/">Production of glycoproteins for structural analyses</a><div>Masamichi Nagae and Yoshiki Yamaguchi.</div><div class="small">Created: September 28, 2021; Last Revision: January 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g127-Alcianblue"><a class="toc-item" href="/books/n/glycopodv2/g127-Alcianblue/">Succinylation-Alcian blue staining for mucins</a><div>Akihiko Kameyama and Weijie Dong.</div><div class="small">Created: October 1, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g128-pyridylamino"><a class="toc-item" href="/books/n/glycopodv2/g128-pyridylamino/">Conversion of pyridylamino sugar chains to corresponding reducing sugar chains</a><div>Shin-ichi Nakakita.</div><div class="small">Created: August 24, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g129-methanolysis"><a class="toc-item" href="/books/n/glycopodv2/g129-methanolysis/">Structural elucidation of oligosaccharides in glycosphingolipids by permethylation and methanolysis</a><div>Saki Itonori.</div><div class="small">Created: September 9, 2021; Last Revision: May 2, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g131-tunicamycin"><a class="toc-item" href="/books/n/glycopodv2/g131-tunicamycin/">Inhibition of <i>N</i>-glycan synthesis by tunicamycin</a><div>Motohiro Nonaka.</div><div class="small">Created: October 1, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g132-DiIlabeling"><a class="toc-item" href="/books/n/glycopodv2/g132-DiIlabeling/">DiI labeling experiment</a><div>Jyoji Morise and Shogo Oka.</div><div class="small">Created: September 7, 2021; Last Revision: March 14, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_massspectro"><a class="toc-item" href="/books/n/glycopodv2/massspectro/">Mass spectrometry (MS)</a><ul id="toc_lst_NBK593839_toc_massspectro" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g133-MSnspectral"><a class="toc-item" href="/books/n/glycopodv2/g133-MSnspectral/">Glycan analysis by simple manual MS<sup>n</sup> spectral matching</a><div>Ken Hanzawa.</div><div class="small">Created: October 20, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g134-NglycanLCMS"><a class="toc-item" href="/books/n/glycopodv2/g134-NglycanLCMS/"><i>N</i>-Glycan profiling by liquid chromatography-mass spectrometry (LC-MS)</a><div>Miyako Nakano.</div><div class="small">Created: October 24, 2021; Last Update: December 21, 2021.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g135-nucleotidesugars"><a class="toc-item" href="/books/n/glycopodv2/g135-nucleotidesugars/">Simultaneous determination of intracellular nucleotide sugars</a><div>Kazuki Nakajima and Naoyuki Taniguchi.</div><div class="small">Created: October 20, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g136-isotopetagging"><a class="toc-item" href="/books/n/glycopodv2/g136-isotopetagging/">Identification of <i>N</i>-glycosylated peptides using glycosylation site-specific stable isotope tagging and liquid chromatography/mass spectrometry</a><div>Hiroyuki Kaji.</div><div class="small">Created: October 1, 2021; Last Revision: March 26, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g137-OglycopeptideMS"><a class="toc-item" href="/books/n/glycopodv2/g137-OglycopeptideMS/"><i>O</i>-Glycopeptide analysis by multi-tandem mass spectrometry</a><div>Masaki Kurogochi.</div><div class="small">Created: August 19, 2021; Last Revision: March 14, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g138-pyrazolone"><a class="toc-item" href="/books/n/glycopodv2/g138-pyrazolone/"><i>O</i>-Linked glycan analysis by β-elimination in the presence of pyrazolone analogue (BEP)</a><div>Yasuro Shinohara, Hisatoshi Hanamatsu, and Jun-ichi Furukawa.</div><div class="small">Created: September 28, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g195-amynolysissia"><a class="toc-item" href="/books/n/glycopodv2/g195-amynolysissia/">Sialic acid linkage-specific alkylamidation via ring-opening aminolysis (aminolysis-SALSA)</a><div>Hisatoshi Hanamatsu, Takashi Nishikaze, and Jun-ichi Furukawa.</div><div class="small">Created: September 29, 2021; Last Update: December 23, 2021.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g139-massdatabase"><a class="toc-item" href="/books/n/glycopodv2/g139-massdatabase/">Identification of intact glycopeptides by liquid chromatography/tandem mass spectrometry followed by database search</a><div>Hiroyuki Kaji.</div><div class="small">Created: October 1, 2021; Last Revision: March 28, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g140-Ionmobilitymass"><a class="toc-item" href="/books/n/glycopodv2/g140-Ionmobilitymass/">Ion mobility mass spectrometry of <i>N</i>-linked glycan</a><div>Kenji Hirose.</div><div class="small">Created: November 6, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g141-glycosphinLC-MS"><a class="toc-item" href="/books/n/glycopodv2/g141-glycosphinLC-MS/">Structural characterization of glycosphingolipids by liquid chromatography-mass spectrometry</a><div>Akemi Suzuki, Takahiro Nitta, Atit Silsirivanit, Naoto Oikawa, Junko Matsuda, and Jin-ichi Inokuchi.</div><div class="small">Created: September 25, 2021; Last Revision: February 27, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g142-conglycoliplcms"><a class="toc-item" href="/books/n/glycopodv2/g142-conglycoliplcms/">Relative content analysis of glycosphingolipids by liquid chromatography-mass spectrometry with multiple reaction monitoring</a><div>Akemi Suzuki, Takahiro Nitta, Atit Silsirivanit, and Jin-ichi Inokuchi.</div><div class="small">Created: September 25, 2021; Last Revision: February 27, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g143-massimaging"><a class="toc-item" href="/books/n/glycopodv2/g143-massimaging/">Mass spectrometry imaging for glycosphingolipids</a><div>Md. Mahmudul Hasan, Mst. Afsana Mimi, Kenji Kikushima, Tomoaki Kahyo, and Mitsutoshi Setou.</div><div class="small">Created: September 29, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g144-cellularglycome"><a class="toc-item" href="/books/n/glycopodv2/g144-cellularglycome/">Cellular glycome analysis</a><div>Jun-ichi Furukawa, Hisatoshi Hanamatsu, and Yasuro Shinohara.</div><div class="small">Created: September 28, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g145-humanwholeserum"><a class="toc-item" href="/books/n/glycopodv2/g145-humanwholeserum/">Glycosphingolipid-glycan analysis of human whole serum/plasma based on an endoglycoceramidase digestion</a><div>Hisatoshi Hanamatsu and Jun-ichi Furukawa.</div><div class="small">Created: September 29, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g146-glycosaminsites"><a class="toc-item" href="/books/n/glycopodv2/g146-glycosaminsites/">Determination of glycosaminoglycan attachment sites by LC-MS/MS</a><div>Fredrik Noborn, Nilsson Jonas, Nikpour Mahnaz, and Larson Göran.</div><div class="small">Created: October 15, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g147-sulfglycosamino"><a class="toc-item" href="/books/n/glycopodv2/g147-sulfglycosamino/">Mass spectrometry analysis of sulfated glycosaminoglycan oligosaccharides</a><div>Shuhei Yamada.</div><div class="small">Created: October 19, 2021; Last Revision: March 15, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_nuclear"><a class="toc-item" href="/books/n/glycopodv2/nuclear/">Nuclear magnetic resonance</a><ul id="toc_lst_NBK593839_toc_nuclear" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g148-nuclearmagnetic"><a class="toc-item" href="/books/n/glycopodv2/g148-nuclearmagnetic/">Determination of glycan structure by nuclear magnetic resonance</a><div>Yoshiki Yamaguchi.</div><div class="small">Created: September 30, 2021; Last Revision: January 29, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_gaschroma"><a class="toc-item" href="/books/n/glycopodv2/gaschroma/">Gas chromatography/liquid chromatography</a><ul id="toc_lst_NBK593839_toc_gaschroma" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g149-gasliquidchro"><a class="toc-item" href="/books/n/glycopodv2/g149-gasliquidchro/">Carbohydrate analysis by gas-liquid chromatography</a><div>Chihiro Sato.</div><div class="small">Created: September 11, 2021; Last Revision: January 26, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g150-fluoresclabel"><a class="toc-item" href="/books/n/glycopodv2/g150-fluoresclabel/">Fluorescent labeling of glycans and high-performance liquid chromatography (HPLC) analysis</a><div>Shin-ichi Nakakita.</div><div class="small">Created: October 7, 2021; Last Revision: January 31, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g151-glucocerebros"><a class="toc-item" href="/books/n/glycopodv2/g151-glucocerebros/">Assay of glucocerebrosidases using high-performance liquid chromatography and fluorescent substrates</a><div>Yasuhiro Hayashi.</div><div class="small">Created: October 6, 2021; Last Revision: May 11, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g152-glycosphsyntha"><a class="toc-item" href="/books/n/glycopodv2/g152-glycosphsyntha/">Assay of glycosphingolipid synthases using high-performance liquid chromatography and fluorescent substrates</a><div>Yasuhiro Hayashi.</div><div class="small">Created: October 6, 2021; Last Revision: May 11, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g153-o-phtaladehyde"><a class="toc-item" href="/books/n/glycopodv2/g153-o-phtaladehyde/">Simultaneous quantification of glucosylceramide and galactosylceramide by high-performance liquid chromatography using <i>o</i>-phtalaldehyde derivatives</a><div>Nozomu Okino and Makoto Ito.</div><div class="small">Created: October 11, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g154-hplcgag"><a class="toc-item" href="/books/n/glycopodv2/g154-hplcgag/">High-performance liquid chromatography analysis of glycosaminoglycans</a><div>Shuhei Yamada and Hidenao Toyoda.</div><div class="small">Created: October 6, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g217-hyalunsatdis"><a class="toc-item" href="/books/n/glycopodv2/g217-hyalunsatdis/">High-performance liquid chromatography analysis of hyaluronan unsaturated disaccharides</a><div>Tomomi Izumikawa and Hidenao Toyoda.</div><div class="small">Created: November 1, 2021; Last Revision: February 11, 2023.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g155-keratansulfate"><a class="toc-item" href="/books/n/glycopodv2/g155-keratansulfate/">High-performance liquid chromatography analysis of keratan sulfate oligosaccharides</a><div>Tomomi Izumikawa and Hidenao Toyoda.</div><div class="small">Created: November 5, 2021; Last Revision: March 29, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g156-mappingN-linked"><a class="toc-item" href="/books/n/glycopodv2/g156-mappingN-linked/">Multidimensional high-performance liquid chromatography-mapping method for <i>N</i>-linked glycan analysis</a><div>Hirokazu Yagi and Koichi Kato.</div><div class="small">Created: January 10, 2022; Last Revision: March 14, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g157-aminobenzoic"><a class="toc-item" href="/books/n/glycopodv2/g157-aminobenzoic/">Two-mode analysis by high-performance liquid chromatography of <i>p</i>-amino benzoic acid ethyl ester-labeled monosaccharides</a><div>Yuka Kobayashi, Hiroto Matsui, and Shunji Natsuka.</div><div class="small">Created: September 29, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g158-standardization"><a class="toc-item" href="/books/n/glycopodv2/g158-standardization/">Standardization of glycan elution on high-performance liquid chromatography</a><div>Ken Hanzawa.</div><div class="small">Created: January 18, 2022; Last Revision: March 24, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_binding"><a class="toc-item" href="/books/n/glycopodv2/binding/">Antibody-based binding assays</a><ul id="toc_lst_NBK593839_toc_binding" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g159-antiglycosamin1"><a class="toc-item" href="/books/n/glycopodv2/g159-antiglycosamin1/">Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [1] ~ Characteristics and epitopes for anti-heparan sulfate antibodies</a><div>Shuhei Yamada and Shuji Mizumoto.</div><div class="small">Created: October 1, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g160-antiglycosamin2"><a class="toc-item" href="/books/n/glycopodv2/g160-antiglycosamin2/">Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [2] ~ Determination of the concentration of heparan sulfate using enzyme-linked immunosorbent assay</a><div>Shuhei Yamada.</div><div class="small">Created: October 5, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g161-antiglycosamin3"><a class="toc-item" href="/books/n/glycopodv2/g161-antiglycosamin3/">Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [3] ~ Immunostaining using anti-heparan sulfate antibody</a><div>Shuhei Yamada.</div><div class="small">Created: October 4, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g162-antiglycosamin4"><a class="toc-item" href="/books/n/glycopodv2/g162-antiglycosamin4/">Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [4] ~ Detection of hyaluronan using biotinylated hyaluronan binding proteins</a><div>Naoki Itano.</div><div class="small">Created: September 28, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g163-antiglycosamin5"><a class="toc-item" href="/books/n/glycopodv2/g163-antiglycosamin5/">Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [5] ~ enzyme-linked immunosorbent assay for hyaluronan quantification</a><div>Shuhei Yamada, Shuji Mizumoto, and Naoki Itano.</div><div class="small">Created: October 4, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g164-immunostkeratan"><a class="toc-item" href="/books/n/glycopodv2/g164-immunostkeratan/">Application of anti-glycosaminoglycan antibody: Immunostaining of keratan sulfate and sulfated <i>N</i>-acetyllactosamine oligosaccharides</a><div>Hitomi Hoshino, Motohiro Kobayashi, and Kenji Uchimura.</div><div class="small">Created: September 9, 2021; Last Revision: March 18, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g165-antichondrsulf"><a class="toc-item" href="/books/n/glycopodv2/g165-antichondrsulf/">Anti-chondroitin sulfate antibodies and their utility for immunostaining analyses</a><div>Tadahisa Mikami and Hiroshi Kitagawa.</div><div class="small">Created: September 22, 2021; Last Revision: January 12, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g166-chondrowestern"><a class="toc-item" href="/books/n/glycopodv2/g166-chondrowestern/">Anti-chondroitin sulfate antibodies and their utility for enzyme-linked immunosorbent assay and western blotting</a><div>Tadahisa Mikami and Hiroshi Kitagawa.</div><div class="small">Created: October 2, 2021; Last Revision: March 15, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g167-kerataninhibit"><a class="toc-item" href="/books/n/glycopodv2/g167-kerataninhibit/">Quantification of keratan sulfate in blood by enzyme-linked immunosorbent assay inhibition assay</a><div>Tomoya Akama, O.</div><div class="small">Created: September 30, 2021; Last Revision: March 23, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g168-mucin21"><a class="toc-item" href="/books/n/glycopodv2/g168-mucin21/">Immunohistochemistry of mucin 21</a><div>Kaori Denda-Nagai, Katrin B. Ishii-Schrade, Yuan Tian, Kyoko Okada, and Tatsuro Irimura.</div><div class="small">Created: October 19, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g169-thinlayergangli"><a class="toc-item" href="/books/n/glycopodv2/g169-thinlayergangli/">Thin-layer chromatography-immunostaining method and enzyme-linked immunosorbent assay for detection of gangliosides by specific antibodies</a><div>Shiori Go and Chihiro Sato.</div><div class="small">Created: September 27, 2021; Last Revision: March 24, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_lectinassay"><a class="toc-item" href="/books/n/glycopodv2/lectinassay/">Lectin-based binding assays</a><ul id="toc_lst_NBK593839_toc_lectinassay" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g170-lectinSPR"><a class="toc-item" href="/books/n/glycopodv2/g170-lectinSPR/">Binding assay of lectins and glycoproteins by surface plasmon resonance</a><div>Yuka Kobayashi.</div><div class="small">Created: December 9, 2021; Last Revision: March 30, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g171-pholiotalecfuc"><a class="toc-item" href="/books/n/glycopodv2/g171-pholiotalecfuc/">Lectin immunohistochemisty with <i>Pholiota squarrosa</i> lectin to evaluate core fucose</a><div>Eiji Miyoshi and Yoshihiro Kamada.</div><div class="small">Created: August 24, 2021; Last Revision: April 1, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g172-histobylectin"><a class="toc-item" href="/books/n/glycopodv2/g172-histobylectin/">Histochemical analysis of cell surface glycans by lectin</a><div>Motohiro Nonaka.</div><div class="small">Created: August 20, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g173-lecmicroarray"><a class="toc-item" href="/books/n/glycopodv2/g173-lecmicroarray/">Glycan profiling by lectin microarray</a><div>Masao Yamada and Jun Hirabayashi.</div><div class="small">Created: July 1, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g174-tissulecarray"><a class="toc-item" href="/books/n/glycopodv2/g174-tissulecarray/">Tissue glycan analysis using lectin microarray</a><div>Atsushi Matsuda.</div><div class="small">Created: October 15, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g175-lectinblot"><a class="toc-item" href="/books/n/glycopodv2/g175-lectinblot/">Lectin blotting</a><div>Motohiro Nonaka and Toshisuke Kawasaki.</div><div class="small">Created: September 10, 2021; Last Revision: March 21, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_others3"><a class="toc-item" href="/books/n/glycopodv2/others3/">Other types of binding assays</a><ul id="toc_lst_NBK593839_toc_others3" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g176-tlcinflurebind"><a class="toc-item" href="/books/n/glycopodv2/g176-tlcinflurebind/">Thin-layer chromatography (TLC)-based assay for determination of influenza virus-host receptor binding specificity</a><div>Yasuo Suzuki and Nongluk Sriwilaijaroen.</div><div class="small">Created: November 24, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g177-bindbacglycolip"><a class="toc-item" href="/books/n/glycopodv2/g177-bindbacglycolip/">Assay of the binding of bacteria to glycosphingolipids</a><div>Shinichi Chisada, Naoyuki Matsunaga, and Makoto Ito.</div><div class="small">Created: September 30, 2021; Last Revision: March 20, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g178-gm1arota"><a class="toc-item" href="/books/n/glycopodv2/g178-gm1arota/">Role of ganglioside GM1a in infection by human rotaviruses</a><div>Yasuo Suzuki and Chao-Tan Guo.</div><div class="small">Created: November 24, 2021; Last Revision: March 20, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g179-sprnorobloodant"><a class="toc-item" href="/books/n/glycopodv2/g179-sprnorobloodant/">Surface plasmon resonance assay to measure binding specificity and relative affinity between noroviruses and synthetic histo-blood group antigens</a><div>Haruko Shirato.</div><div class="small">Created: November 12, 2021; Last Revision: March 22, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_others4"><a class="toc-item" href="/books/n/glycopodv2/others4/">Other protocols for glycan characterization</a><ul id="toc_lst_NBK593839_toc_others4" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g180-partgpitritonx"><a class="toc-item" href="/books/n/glycopodv2/g180-partgpitritonx/">Partitioning of glycosylphosphatidylinositol (GPI)-anchored proteins with Triton X-114</a><div>Morihisa Fujita, Yusuke Maeda, and Taroh Kinoshita.</div><div class="small">Created: September 17, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g181-piplcgpi"><a class="toc-item" href="/books/n/glycopodv2/g181-piplcgpi/">Phosphatidylinositol-specific phospholipase C (PI-PLC) treatment of glycosylphosphatidylinositol (GPI)-anchored proteins on the cell surface of intact cells</a><div>Morihisa Fujita, Yusuke Maeda, and Taroh Kinoshita.</div><div class="small">Created: September 17, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g182-pulsechasedaf"><a class="toc-item" href="/books/n/glycopodv2/g182-pulsechasedaf/">Pulse-chase transport analysis of decay accelerating factor (DAF) from the ER to the Golgi</a><div>Morihisa Fujita, Yusuke Maeda, and Taroh Kinoshita.</div><div class="small">Created: September 17, 2021; Last Revision: March 25, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g183-cellularsynth"><a class="toc-item" href="/books/n/glycopodv2/g183-cellularsynth/">Cellular synthesis of oligosaccharide using saccharide primer</a><div>Toshinori Sato.</div><div class="small">Created: October 15, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g184-colorreaction"><a class="toc-item" href="/books/n/glycopodv2/g184-colorreaction/">Color reaction of carbohydrates</a><div>Shunji Natsuka.</div><div class="small">Created: September 17, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g185-afm"><a class="toc-item" href="/books/n/glycopodv2/g185-afm/">Atomic force microscopic observation of reconstituted lipid bilayers containing glycosphingolipids</a><div>Teruhiko Matsubara and Toshinori Sato.</div><div class="small">Created: October 15, 2021; Last Revision: March 16, 2022.</div></li></ul></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_chemicalanal"><a class="toc-item" href="/books/n/glycopodv2/chemicalanal/">Chemical analysis</a><ul id="toc_lst_NBK593839_toc_chemicalanal" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_degradation"><a class="toc-item" href="/books/n/glycopodv2/degradation/">Degradation</a><ul id="toc_lst_NBK593839_toc_degradation" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g186-acetylysis"><a class="toc-item" href="/books/n/glycopodv2/g186-acetylysis/">Partial acetolysis for glycan linkage analysis</a><div>Shunji Natsuka and Shin-ichi Nakakita.</div><div class="small">Created: September 13, 2021; Last Revision: December 26, 2021.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g187-smithdgrdtn"><a class="toc-item" href="/books/n/glycopodv2/g187-smithdgrdtn/">Smith degradation for glycan linkage analysis</a><div>Shunji Natsuka and Shin-ichi Nakakita.</div><div class="small">Created: September 14, 2021; Last Revision: January 26, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g188-regiospecific"><a class="toc-item" href="/books/n/glycopodv2/g188-regiospecific/">Preparation of regioselectively 6-<i>O</i>-desulfated glycans by a non-destructive chemical method using silylating reagents</a><div>Ryo Takano.</div><div class="small">Created: September 14, 2021; Last Revision: February 3, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g189-hydrazynolysis"><a class="toc-item" href="/books/n/glycopodv2/g189-hydrazynolysis/">Hydrazinolysis using hydrazine monohydrate reducing sugar chains</a><div>Shin-ichi Nakakita.</div><div class="small">Created: August 24, 2021; Last Revision: March 21, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_labeling"><a class="toc-item" href="/books/n/glycopodv2/labeling/">Labeling</a><ul id="toc_lst_NBK593839_toc_labeling" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g190-releasengly"><a class="toc-item" href="/books/n/glycopodv2/g190-releasengly/">Release of <i>N</i>-glycans by hydrazinolysis</a><div>Shin-ichi Nakakita.</div><div class="small">Created: August 24, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g191-labelingsia"><a class="toc-item" href="/books/n/glycopodv2/g191-labelingsia/">Specific labeling and chemical analysis of sialic acid and sialyloligo/polymers</a><div>Chihiro Sato.</div><div class="small">Created: September 11, 2021; Last Revision: March 24, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g130-metabosphingo"><a class="toc-item" href="/books/n/glycopodv2/g130-metabosphingo/">Metabolic labeling of sphingolipids</a><div>Motohiro Tani, Hironobu Komori, and Makoto Ito.</div><div class="small">Created: September 15, 2021; Last Revision: May 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g192-fluorolabel"><a class="toc-item" href="/books/n/glycopodv2/g192-fluorolabel/">Enzymatic fluorescent labeling of 6-gala (neogala) series glycosphingolipids-oligosaccharides</a><div>Yohei Ishibashi and Makoto Ito.</div><div class="small">Created: October 5, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g193-quanticer"><a class="toc-item" href="/books/n/glycopodv2/g193-quanticer/">Quantitative determination of ceramide</a><div>Nozomu Okino and Makoto Ito.</div><div class="small">Created: September 28, 2021; Last Revision: March 21, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g194-quantifysm"><a class="toc-item" href="/books/n/glycopodv2/g194-quantifysm/">Quantitative determination of sphingomyelin</a><div>Nozomu Okino and Makoto Ito.</div><div class="small">Created: September 28, 2021; Last Revision: March 21, 2022.</div></li></ul></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_chemicalsynth"><a class="toc-item" href="/books/n/glycopodv2/chemicalsynth/">Chemical synthesis</a><ul id="toc_lst_NBK593839_toc_chemicalsynth" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_monosaccharide"><a class="toc-item" href="/books/n/glycopodv2/monosaccharide/">Monosaccharide protection</a><ul id="toc_lst_NBK593839_toc_monosaccharide" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g196-acetylation"><a class="toc-item" href="/books/n/glycopodv2/g196-acetylation/"><i>O</i>-Acetylation using acetic anhydride in pyridine</a><div>Akihiro Imamura.</div><div class="small">Created: October 6, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g197-deacetylation"><a class="toc-item" href="/books/n/glycopodv2/g197-deacetylation/">De-<i>O</i>-acetylation using sodium methoxide</a><div>Akihiro Imamura.</div><div class="small">Created: October 6, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g198-benzylprtct"><a class="toc-item" href="/books/n/glycopodv2/g198-benzylprtct/">Benzylidene protection of diol</a><div>Shino Manabe.</div><div class="small">Created: September 30, 2021; Last Revision: April 1, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g199-benzylcleave"><a class="toc-item" href="/books/n/glycopodv2/g199-benzylcleave/">Removal of benzylidene group</a><div>Hidenori Tanaka.</div><div class="small">Created: October 6, 2021; Last Revision: April 5, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g200-reductiveopen"><a class="toc-item" href="/books/n/glycopodv2/g200-reductiveopen/">Reductive opening of benzylidene group</a><div>Hidenori Tanaka.</div><div class="small">Created: October 6, 2021; Last Revision: April 5, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g201-williamson"><a class="toc-item" href="/books/n/glycopodv2/g201-williamson/">Benzylation of hydroxyl groups by Williamson reaction</a><div>Hiromune Ando.</div><div class="small">Created: October 6, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g202-introtbdps"><a class="toc-item" href="/books/n/glycopodv2/g202-introtbdps/">Introduction of <i>tert</i>-butyldiphenylsilyl (TBDPS) group</a><div>Hidenori Tanaka.</div><div class="small">Created: October 6, 2021; Last Revision: April 5, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g203-trocamino"><a class="toc-item" href="/books/n/glycopodv2/g203-trocamino/">Introduction of 2,2,2-trichloroethoxycarbonyl (Troc) group at amino group</a><div>Hidenori Tanaka.</div><div class="small">Created: October 6, 2021; Last Revision: April 5, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_glycosyl"><a class="toc-item" href="/books/n/glycopodv2/glycosyl/">Glycosyl donor preparation</a><ul id="toc_lst_NBK593839_toc_glycosyl" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g204-phenylthio"><a class="toc-item" href="/books/n/glycopodv2/g204-phenylthio/">Synthesis of phenylthioglycoside</a><div>Akihiro Imamura.</div><div class="small">Created: October 6, 2021; Last Revision: March 22, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g205-convertthio"><a class="toc-item" href="/books/n/glycopodv2/g205-convertthio/">Conversion of thioglycosides into hemiacetals</a><div>Hiromune Ando.</div><div class="small">Created: October 6, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g206-synthimidate"><a class="toc-item" href="/books/n/glycopodv2/g206-synthimidate/">Synthesis of glycosyl imidate donor</a><div>Hiromune Ando.</div><div class="small">Created: October 6, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g207-synthglycophos"><a class="toc-item" href="/books/n/glycopodv2/g207-synthglycophos/">Synthesis of glycosyl phosphate donor</a><div>Hiromune Ando.</div><div class="small">Created: October 6, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g208-synthglyfluo"><a class="toc-item" href="/books/n/glycopodv2/g208-synthglyfluo/">Synthesis of glycosyl fluoride donor</a><div>Akihiro Imamura.</div><div class="small">Created: October 6, 2021; Last Revision: March 17, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g209-azidochrol"><a class="toc-item" href="/books/n/glycopodv2/g209-azidochrol/">Azidochrolination reaction of tri-<i>O</i>-acetyl-D-galactal</a><div>Kotaro Kanzaki and Osamu Kanie.</div><div class="small">Created: October 23, 2021; Last Revision: March 13, 2022.</div></li></ul></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_glycosidation"><a class="toc-item" href="/books/n/glycopodv2/glycosidation/">Glycosidation</a><ul id="toc_lst_NBK593839_toc_glycosidation" class="simple-list toc bktoc_lst_exp"><li class="half_rhythm" id="toc_itm_NBK593839_toc_g210-thioglycoside"><a class="toc-item" href="/books/n/glycopodv2/g210-thioglycoside/">Glycosidation using thioglycoside donor</a><div>Naoko Komura.</div><div class="small">Created: October 6, 2021; Last Revision: March 19, 2022.</div></li><li class="half_rhythm" id="toc_itm_NBK593839_toc_g211-trichloroacet"><a class="toc-item" href="/books/n/glycopodv2/g211-trichloroacet/">Glycosidation using trichloroacetimidate donor</a><div>Naoko Komura.</div><div class="small">Created: October 6, 2021; 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