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<meta name="robots" content="INDEX,FOLLOW,NOARCHIVE" /><meta name="citation_inbook_title" content="Coffee Break: Tutorials for NCBI Tools [Internet]" /><meta name="citation_title" content="Fluorescent timer" /><meta name="citation_publisher" content="National Center for Biotechnology Information (US)" /><meta name="citation_date" content="2001/01/22" /><meta name="citation_author" content="Laura Dean" /><meta name="citation_author" content="Johanna McEntyre" /><meta name="citation_fulltext_html_url" content="https://www.ncbi.nlm.nih.gov/books/NBK2319/" /><link rel="schema.DC" href="http://purl.org/DC/elements/1.0/" /><meta name="DC.Title" content="Fluorescent timer" /><meta name="DC.Type" content="Text" /><meta name="DC.Publisher" content="National Center for Biotechnology Information (US)" /><meta name="DC.Contributor" content="Laura Dean" /><meta name="DC.Contributor" content="Johanna McEntyre" /><meta name="DC.Date" content="2001/01/22" /><meta name="DC.Identifier" content="https://www.ncbi.nlm.nih.gov/books/NBK2319/" /><meta name="description" content="There are many ways to monitor the onset of gene expression, but so far it has been impossible to detect its down-regulation. This problem might have been solved now, as Terskikh and colleagues report in Science a simple method to follow promoter activity." /><meta name="og:title" content="Fluorescent timer" /><meta name="og:type" content="book" /><meta name="og:description" content="There are many ways to monitor the onset of gene expression, but so far it has been impossible to detect its down-regulation. This problem might have been solved now, as Terskikh and colleagues report in Science a simple method to follow promoter activity." /><meta name="og:url" content="https://www.ncbi.nlm.nih.gov/books/NBK2319/" /><meta name="og:site_name" content="NCBI Bookshelf" /><meta name="og:image" content="https://www.ncbi.nlm.nih.gov/corehtml/pmc/pmcgifs/bookshelf/thumbs/th-coffeebrk-lrg.png" /><meta name="twitter:card" content="summary" /><meta name="twitter:site" content="@ncbibooks" /><meta name="bk-non-canon-loc" content="/books/n/coffeebrk/A28/" /><link rel="canonical" href="https://www.ncbi.nlm.nih.gov/books/NBK2319/" /><link rel="stylesheet" href="/corehtml/pmc/css/figpopup.css" type="text/css" media="screen" /><link rel="stylesheet" href="/corehtml/pmc/css/bookshelf/2.26/css/books.min.css" type="text/css" /><link rel="stylesheet" href="/corehtml/pmc/css/bookshelf/2.26/css/books_print.min.css" type="text/css" media="print" /><style type="text/css">p a.figpopup{display:inline !important} .bk_tt {font-family: monospace} .first-line-outdent .bk_ref {display: inline} .body-content h2, .body-content .h2 {border-bottom: 1px solid #97B0C8} .body-content h2.inline {border-bottom: none} a.page-toc-label , .jig-ncbismoothscroll a {text-decoration:none;border:0 !important} .temp-labeled-list .graphic {display:inline-block !important} .temp-labeled-list img{width:100%}</style><script type="text/javascript" src="/corehtml/pmc/js/jquery.hoverIntent.min.js"> </script><script type="text/javascript" src="/corehtml/pmc/js/common.min.js?_=3.18"> </script><script type="text/javascript" src="/corehtml/pmc/js/large-obj-scrollbars.min.js"> </script><script type="text/javascript">window.name="mainwindow";</script><script type="text/javascript" src="/corehtml/pmc/js/bookshelf/2.26/book-toc.min.js"> </script><script type="text/javascript" src="/corehtml/pmc/js/bookshelf/2.26/books.min.js"> </script><meta name="book-collection" content="NONE" />
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<div class="pre-content"><div><div class="bk_prnt"><p class="small">NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.</p><p>Dean L, McEntyre J, editors. Coffee Break: Tutorials for NCBI Tools [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 1999-. </p></div><div class="iconblock clearfix whole_rhythm no_top_margin bk_noprnt"><a class="img_link icnblk_img" title="Table of Contents Page" href="/books/n/coffeebrk/"><img class="source-thumb" src="/corehtml/pmc/pmcgifs/bookshelf/thumbs/th-coffeebrk-lrg.png" alt="Cover of Coffee Break" height="100px" width="80px" /></a><div class="icnblk_cntnt eight_col"><h2>Coffee Break: Tutorials for NCBI Tools [Internet].</h2><a data-jig="ncbitoggler" href="#__NBK2319_dtls__">Show details</a><div style="display:none" class="ui-widget" id="__NBK2319_dtls__"><div>Dean L, McEntyre J, editors.</div><div>Bethesda (MD): <a href="https://www.ncbi.nlm.nih.gov/" ref="pagearea=page-banner&targetsite=external&targetcat=link&targettype=publisher">National Center for Biotechnology Information (US)</a>; 1999-.</div></div><div class="half_rhythm"><ul class="inline_list"><li style="margin-right:1em"><a class="bk_cntns" href="/books/n/coffeebrk/">Contents</a></li></ul></div><div class="bk_noprnt"><form method="get" action="/books/n/coffeebrk/" id="bk_srch"><div class="bk_search"><label for="bk_term" class="offscreen_noflow">Search term</label><input type="text" title="Search this book" id="bk_term" name="term" value="" data-jig="ncbiclearbutton" /> <input type="submit" class="jig-ncbibutton" value="Search this book" submit="false" style="padding: 0.1em 0.4em;" /></div></form></div></div><div class="icnblk_cntnt two_col"><div class="pagination bk_noprnt"><a class="active page_link prev" href="/books/n/coffeebrk/A29/" title="Previous page in this title">< Prev</a><a class="active page_link next" href="/books/n/coffeebrk/A27/" title="Next page in this title">Next ></a></div></div></div></div></div>
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<div class="main-content lit-style" itemscope="itemscope" itemtype="http://schema.org/CreativeWork"><div class="meta-content fm-sec"><h1 id="_NBK2319_"><span class="title" itemprop="name">Fluorescent timer</span></h1><div class="subtitle"> the E5 mutant of the red coral protein drFP583 changes its fluorescence from green to red over time</div><p class="small">Created: <span itemprop="datePublished">January 22, 2001</span>.</p></div><div class="body-content whole_rhythm" itemprop="text"><p>There are many ways to monitor the onset of gene expression, but so far it has been impossible to detect its down-regulation. This problem might have been solved now, as Terskikh and colleagues report in <a href="/htbin-post/Entrez/query?db=m&form=6&Dopt=r&uid=11090358" ref="pagearea=body&targetsite=external&targetcat=link&targettype=uri"><i>Science</i></a> a simple method to follow promoter activity.</p><p>Last year, a red fluorescent protein (drFP583) was identified in tropical corals, further increasing the wide spectrum of possibilities to light up cells in different colors. Not satisfied with just one color, Terskikh and colleagues introduced random mutations into drFP583, and found one mutant (called E5) that changes its fluorescence from green to red in a time-dependent manner. As E5 switches from green to red fluorescence over time, it can be used as a timer for gene expression. During the first hours of activity of a promoter, green fluorescence is predominant, whereas sustained activity of the promoter leads to a mixture of green and red fluorescence. A few hours after the promoter is turned off, only red fluorescence remains.</p><div class="iconblock whole_rhythm clearfix ten_col fig" id="figA557" co-legend-rid="figlgndA557"><a href="/books/NBK2319/figure/A557/?report=objectonly" target="object" title="Figure" class="img_link icnblk_img figpopup" rid-figpopup="figA557" rid-ob="figobA557"><img class="small-thumb" src="/books/NBK2319/bin/cb18_big.gif" src-large="/books/NBK2319/bin/cb18_big.jpg" alt="The change in fluorescence of E5 over time in C. elegans." /></a><div class="icnblk_cntnt" id="figlgndA557"><h4 id="A557"><a href="/books/NBK2319/figure/A557/?report=objectonly" target="object" rid-ob="figobA557">Figure</a></h4><p class="float-caption no_bottom_margin">The change in fluorescence of E5 over time in <i>C. elegans.</i> The <i>E5</i> mutant was placed under the control of a heat shock promoter and injected into <i>C. elegans</i> embryos. Green fluoresence was detected 2 hours into the recovery phase following a standard heat <a href="/books/NBK2319/figure/A557/?report=objectonly" target="object" rid-ob="figobA557">(more...)</a></p></div></div><p>Terskikh and colleagues verified these predictions in three experimental systems. First they monitored up- and down-regulation of E5 expression in Tet-on and Tet-off mammalian expression systems. Then they followed the activity of a heat-shock promoter during heat-induced stress of<i>Caenorhabditis elegans.</i> Last, they traced the expression of a homeobox gene involved in the patterning of anterior structures in <i>Xenopus laevis.</i> In all cases, green fluorescence correctly indicated the onset of gene expression and was replaced with red fluorescence when expression ceased.</p><p>So after decades of blue-stained embryos, we'll now have to get used to seeing gene expression in green and red.</p><p>Story contributed by Raluca Gagescu, <a href="http://www.nature.com/nrm/" ref="pagearea=body&targetsite=external&targetcat=link&targettype=uri"><u>Nature Reviews Molecular Cell Biology</u></a>
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</p><p>
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</p><div id="A402" class="box"><h3><span class="title">VAST search for structures similar to E5</span></h3><p>Created: January 22, 2001</p><p>Click on the link below to start an html tutorial.</p><p>
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Search for structures similar to E5</p></div><div id="bk_toc_contnr"></div></div></div>
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<div class="post-content"><div><div class="half_rhythm"><a href="/books/about/copyright/">Copyright Notice</a></div><div class="small"><span class="label">Bookshelf ID: NBK2319</span></div><div style="margin-top:2em" class="bk_noprnt"><a class="bk_cntns" href="/books/n/coffeebrk/">Contents</a><div class="pagination bk_noprnt"><a class="active page_link prev" href="/books/n/coffeebrk/A29/" title="Previous page in this title">< Prev</a><a class="active page_link next" href="/books/n/coffeebrk/A27/" title="Next page in this title">Next ></a></div></div></div></div>
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<a href="/Taxonomy/CommonTree/wwwcmt.cgi?id=86600&id=6100" ref="pagearea=document-links&targetsite=external&targetcat=link&targettype=uri">Taxonomic tree of jelly fish & red coral</a>
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