2152 lines
73 KiB
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2152 lines
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<span class="a2a_kit a2a_kit_size_32 addtoany_list" data-a2a-url="https://www.nibib.nih.gov/labs-at-nibib/instant-structured-illumination-microscope-isim-total-internal-reflection-fluorescence-sim-tirf-sim" data-a2a-title="Instant Structured Illumination Microscope (ISIM)/ Total Internal Reflection Fluorescence SIM (TIRF-SIM)"><a class="a2a_button_email"><span class="a2a_svg a2a_s_a2a a2a_s_email a2a-box"><img src="/themes/custom/nibib/assets/img/email-a2a.svg" border="0" alt="Facebook" width="27" height="27"></span></a><a class="a2a_button_facebook"><span class="a2a_svg a2a_s_a2a a2a_s_facebook a2a-box"><img src="/themes/custom/nibib/assets/img/facebook-a2a.svg" border="0" alt="Facebook" width="27" height="27"></span></a><a class="a2a_button_linkedin"><span class="a2a_svg a2a_s_a2a a2a_s_linkedin a2a-box"><img src="/themes/custom/nibib/assets/img/linkedin-a2a.svg" border="0" alt="Facebook" width="27" height="27"></span></a><a class="a2a_button_twitter"><span class="a2a_svg a2a_s_a2a a2a_s_x a2a-box"><img src="/themes/custom/nibib/assets/img/x-a2a.svg" border="0" alt="Facebook" width="27" height="27"></span
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<span property="schema:name">Instant Structured Illumination Microscope (ISIM)/ Total Internal Reflection Fluorescence SIM (TIRF-SIM)</span>
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<span class="staff-card--header--name"><a href="/about-nibib/staff/jiji-chen" title="View Jiji Chen's profile">Jiji Chen</a>
|
||
, Ph.D.
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<div property="schema:text" class="field field--name-body field--type-text-with-summary field--label-hidden field__item"><p>The ISIM is a fluorescence microscope that combines rapid image acquisition (100Hz) with a resolution roughly double that of a confocal microscope in all three dimensions. Unlike other structured illumination microscopes, the resolution is enhanced via hardware in a single frame acquisition rather than computationally processing multiple images, effectively making this an ‘instantaneous’ structured illumination microscope. Recently, the Shroff Lab has extended the capability of this microscope to rapid, super-resolution TIRF. </p>
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<p>Spatial resolution: 140nm lateral, 350nm axial</p>
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<p>References:<br>1. Instant super-resolution imaging in live cells and embryos via analog image processing. Nat Methods. 2013 Nov;10(11):1122-6.<br>2. Single-shot super-resolution total internal reflection fluorescence microscopy. Nat Methods. 2018 Jun;15(6):425-428.</p>
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