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<span>Histology Unit</span>
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<img src="/sites/default/files/styles/large/public/2019-06/Baer_120202_0134-Edit.jpg?itok=pE0m3alH" alt="Researcher wearing blue latex gloves and handling eye specimens" />
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<h2>About our work</h2>
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<p><strong>The Histology Unit is part of the </strong><a href="/research/research-labs-and-branches/biological-imaging-core-facility"><strong>Biological Imaging Core Facility</strong></a><strong>.</strong></p><p><strong>Facilities and equipment</strong></p><p>The National Eye Institute's Histology Unit provides expertise and up-to-date technical services for histological (under a light microscope or electron microscope) and cytological analyses for all of NEI and other NIH scientists.</p><p><strong>Services</strong></p><p>The Unit staff provides technical assistance in the development and optimization of histopathological and morphometric techniques, including the following:<br>Sample preparation and sectioning</p><ul><li>Fixation of cells, tissues and organs</li><li>Grossing tissues and organs, particularly the eyes (human and animal)</li><li>Embedding frozen tissues or organs in OCT (optimal cutting temperature)</li><li>Embedding samples in methacrylate or paraffin for light microscopy</li><li>Embedding samples in resin for electron microscopy</li><li>Sectioning frozen samples with a cryostat</li><li>Sectioning of embedded samples with a microtome or ultramicrotome</li></ul><p><strong>Slide processing</strong></p><ul><li>Routine histological stains such as H&E or PAS II and stains such as Gram stains, AFB, silver, etc.</li><li>Immunostaining of human cells, tissues and organs with enzyme histochemistry (acid phosphatase, alkaline phosphatase, HRP, ABC avidin-biotin-complex)</li></ul>
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<h2>Histology Unit key staff</h2>
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<div class="views-element-container">
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Key staff table
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<th id="view-title-table-column" class="views-field views-field-title" scope="col">Name</th>
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<th id="view-field-email-table-column" class="views-field views-field-field-email" scope="col">Email</th>
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<td headers="view-title-table-column" class="views-field views-field-title"><a href="/research/research-labs-and-branches/we-are-nei-intramural/mercedes-campos" hreflang="en">Mercedes Campos, M.D.</a> </td>
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<td headers="view-field-lab-staff-title-table-column" class="views-field views-field-field-lab-staff-title">Unit Chief </td>
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<td headers="view-field-email-table-column" class="views-field views-field-field-email"><a href="mailto:CamposM@nei.nih.gov">CamposM@nei.nih.gov</a> </td>
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<td headers="view-field-phone-table-column" class="views-field views-field-field-phone">301-496-3043 </td>
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<td headers="view-title-table-column" class="views-field views-field-title"><a href="/research/research-labs-and-branches/we-are-nei-intramural/loxene-duncan" hreflang="en">Loxene Duncan</a> </td>
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<td headers="view-field-lab-staff-title-table-column" class="views-field views-field-field-lab-staff-title">Histologist </td>
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<td headers="view-field-email-table-column" class="views-field views-field-field-email"><a href="mailto:loxene.duncan@nih.gov">loxene.duncan@nih.gov</a> </td>
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<td headers="view-field-phone-table-column" class="views-field views-field-field-phone">301-594-7063 </td>
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<td headers="view-title-table-column" class="views-field views-field-title"><a href="/research/research-labs-and-branches/we-are-nei-intramural/iris-wise" hreflang="en">Iris Wise</a> </td>
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<td headers="view-field-lab-staff-title-table-column" class="views-field views-field-field-lab-staff-title">Lead Histologist </td>
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<td headers="view-field-email-table-column" class="views-field views-field-field-email"><a href="mailto:milleri@intra.nei.nih.gov">milleri@intra.nei.nih.gov</a> </td>
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<td headers="view-field-phone-table-column" class="views-field views-field-field-phone">301-594-7063 </td>
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<div class="js-view-dom-id-15e1206cb389228c945bce4682acdb59d40f32205ffab9d95f55a7d5b6808f42 lab-alumni">
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<h2 class="c-callout__heading">Research at NEI</h2>
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<ul><li><a href="/research/research-labs-and-branches">Research Labs and Branches</a></li><li><a href="/research/research-labs-and-branches/we-are-nei-intramural">Principal Investigators</a></li><li><a href="/grants-and-training/training-nei">Training at NEI</a></li><li><a href="/about/news-and-events/news">Research News</a></li></ul>
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<p class="views-element-container c-last-updated" id="block-views-block-nei-last-updated-block-1"><strong>Last updated:</strong> December 18, 2024</p>
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<aside class="r-sidebar" role="complementary">
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<div class="c-sidebar-widget">
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<div class="c-chief">
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<div class="o-table--sortable js-view-dom-id-ddecdbc62c60b4dfd035c25e00898a0a1879cf88e07399ab92c8214e7dd3a260 lab-staff">
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<div class="views-field views-field-field-lab-staff-headshot"><div class="field-content c-chief__image">
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<img loading="lazy" src="/sites/default/files/styles/thumbnail/public/2023-01/Picture1.jpg?itok=5fX5jKD9" width="200" height="200" alt="Maria Campos" />
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</div></div><div class="views-field views-field-view-node"><span class="field-content c-chief__bio"><p class="c-chief__title"><a href="/research/research-labs-and-branches/we-are-nei-intramural/mercedes-campos" hreflang="en">Mercedes Campos, M.D.</a></p>
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<p></p>
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<p class="c-chief__department-title">Unit Chief</p>
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<p class="c-chief__department-title"></p>
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<p class="c-chief__email"><a href="mailto:CamposM@nei.nih.gov">Email</a> </p>
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<a href="/research/research-labs-and-branches/we-are-nei-intramural/mercedes-campos" class="c-link__link">View bio</a></span></div></div>
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<span>Histology Unit</span>
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6 Center Drive<br>
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Building 6, Room 131A
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<br>
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Bethesda,
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Maryland
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20892-0606
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<div><span>Phone:</span> 301-496-3043</div>
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<div><span>Fax:</span> 301-480-7939</div>
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