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<h1>
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<span>Histology Unit</span>
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<img src="/sites/default/files/styles/large/public/2019-06/Baer_120202_0134-Edit.jpg?itok=pE0m3alH" alt="Researcher wearing blue latex gloves and handling eye specimens" />
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<h2>About our work</h2>
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<p><strong>The Histology Unit is part of the </strong><a href="/research/research-labs-and-branches/biological-imaging-core-facility"><strong>Biological Imaging Core Facility</strong></a><strong>.</strong></p><p><strong>Facilities and equipment</strong></p><p>The National Eye Institute's Histology Unit provides expertise and up-to-date technical services for histological (under a light microscope or electron microscope) and cytological analyses for all of NEI and other NIH scientists.</p><p><strong>Services</strong></p><p>The Unit staff provides technical assistance in the development and optimization of histopathological and morphometric techniques, including the following:<br>Sample preparation and sectioning</p><ul><li>Fixation of cells, tissues and organs</li><li>Grossing tissues and organs, particularly the eyes (human and animal)</li><li>Embedding frozen tissues or organs in OCT (optimal cutting temperature)</li><li>Embedding samples in methacrylate or paraffin for light microscopy</li><li>Embedding samples in resin for electron microscopy</li><li>Sectioning frozen samples with a cryostat</li><li>Sectioning of embedded samples with a microtome or ultramicrotome</li></ul><p><strong>Slide processing</strong></p><ul><li>Routine histological stains such as H&E or PAS II and stains such as Gram stains, AFB, silver, etc.</li><li>Immunostaining of human cells, tissues and organs with enzyme histochemistry (acid phosphatase, alkaline phosphatase, HRP, ABC avidin-biotin-complex)</li></ul>
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<h2>Histology Unit key staff</h2>
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<div class="c-callout__content">
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<h2 class="c-callout__heading">Research at NEI</h2>
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<ul><li><a href="/research/research-labs-and-branches">Research Labs and Branches</a></li><li><a href="/research/research-labs-and-branches/we-are-nei-intramural">Principal Investigators</a></li><li><a href="/grants-and-training/training-nei">Training at NEI</a></li><li><a href="/about/news-and-events/news">Research News</a></li></ul>
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<p class="views-element-container c-last-updated" id="block-views-block-nei-last-updated-block-1"><strong>Actualización más reciente:</strong> 18 de diciembre de 2024</p>
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Bethesda,
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Maryland
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20892-0606
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