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Transmission Electron Microscopy Unit
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<span>Transmission Electron Microscopy Unit</span>
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<img src="/sites/default/files/styles/large/public/2023-03/Optic%20nerve%20cross%20section-crop.jpg?itok=Q2f_7Bbz" alt="Transmission electron microscopy of a section of mouse optic nerve taken at 20,000X magnification. Photo by Mones Abu-Asab, PhD" />
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<p><strong>The Transmission Electron Microscopy Unit is part of the</strong> <a href="/research/research-labs-and-branches/biological-imaging-core-facility"><strong>Biological Imaging Core Facility</strong></a><strong>.</strong></p><p><strong>Facilities and Equipment</strong></p><p>The Transmission Electron Microscopy Unit (TEM Unit), located in Building 6 on the NIH main campus, is a fully equipped lab that provides the ultrastructural needs of the institutes researchers and their collaborators. Our equipment includes a JEOL JEM-1010 transmission electron microscope (TEM), a Leica UC6 ultramicrotome, and AMT digital camera mounted on the TEM. We aim to provide the highest quality microscopy and analytical consultation to our users and collaborators.</p><p><strong>Services</strong></p><ul><li>Provides various fixatives as appropriate</li><li>Embedding of specimens in epoxy or acrylic resins</li><li>Ultrathin sectioning of resin embedded specimens</li><li>Immunolabeling of acrylic-embedded tissues</li><li>Negative staining of whole mounts such as purified exosomes, proteins, viruses, vesicles…etc.</li><li>Training in EM sample preparation techniques and electron microscope operation</li></ul><p>Please find sample submission instructions on the NEI intranet: <a href="https://intranet.nei.nih.gov/int/Pages/TEM.aspx">https://intranet.nei.nih.gov/int/Pages/TEM.aspx</a></p>
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