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<span>Ophthalmic Genomics Laboratory</span>
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<h2>About our work</h2>
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<p>The Ophthalmic Genomics Laboratory (OGL) manages biobanking and clinical molecular genetic testing of biospecimens in support of NEI clinical center protocols. The goals and objectives of the OGL are:</p><ul><li>Facilitate clinical and research molecular genetic testing for patients seen in the NEI clinics.</li><li>Maintain clinical biospecimens, including DNA, RNA, and skin- and blood-derived cell lines from patients.</li><li>Next-generation sequencing and bioinformatics pipeline for exome and genome analysis, including processing, alignment, variant calling, and variant prioritization.</li><li>Improve clinical DNA variant classification to define causal variants underlying human heritable vision disorders, including population-based analyses and functional laboratory studies.</li><li>Identify and characterize novel disease genes.</li><li class="MsoNormal"><span>Better understand genetic risk factors linked to chronic conditions such as age-related macular degeneration (AMD) and diabetic retinopathy (a leading cause of blindness in individuals with diabetes).</span></li><li>Biospecimen and diagnostic testing support for multiple NEI clinical protocols, including NCT02077894, NCT01432847, NCT00378742, and NCT02471287.</li></ul><p>The Ophthalmic Genomics Laboratory is a fully integrated, clinical functional genomic laboratory supporting NEI clinical care, research efforts, and extramural collaborations. DNA samples from over 3000 study participants and more than 30 patient cell lines are maintained within the laboratory. Represented heritable ocular conditions include but are not limited to: Achromatopsia, Albinism, Aniridia, Coloboma, Microphthalmia, Anophthalmia, Axenfeld-Rieger Syndrome, Best Disease, Bietti Crystalline Dystrophy, Choroideremia, Cone Rod Dystrophy, Congenital Stationary Night Blindness, Corneal Dystrophy, Juvenile X-linked Retinoschisis, Leber Hereditary Optic Neuropathy (LHON), mitochondrial DNA disorders, Optic Atrophy Type 1, Pattern Dystrophy, <em>PNPLA6</em> disorders, Retinitis Pigmentosa, Sorsby Fundus Dystrophy, Stickler Syndrome and Stargardt Disease, and Usher Syndrome.</p><p>Specific tests are performed in-house via CLIA-certified genetic testing, including for <em>RS1</em> X-linked Retinoschisis (XLRS), <em>OPN1LW/OPN1MW</em> Blue Cone Monochromacy (BCM), and site-directed testing for familial segregation and research finding confirmation. Clinical reports are returned to clinic, and clinical phenotyping and molecular diagnostic results are directly compared and discussed in regular clinical molecular rounds meetings.</p><p>The OGL has also developed a bioinformatics pipeline for processing next-generation sequencing data, including panel, exome, and genome datasets, for annotating different variation types such as single nucleotide variants, insertions-deletions, large deletions, and duplications, and structural variations including translocations. Variant prioritization tools are developed by the lab using large disease cohorts, internal variant frequencies, and development of pipeline annotations such as variation constraint, in silico predictors, and published epigenomic datasets.</p>
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<h2>Ophthalmic Genomics Laboratory key staff</h2>
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<ul><li><a href="/research/research-labs-and-branches">Research Labs and Branches</a></li><li><a href="/research/research-labs-and-branches/we-are-nei-intramural">Principal Investigators</a></li><li><a href="/grants-and-training/training-nei">Training at NEI</a></li><li><a href="/about/news-and-events/news">Research News</a></li></ul>
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<p class="views-element-container c-last-updated" id="block-views-block-nei-last-updated-block-1"><strong>Actualización más reciente:</strong> 24 de febrero de 2025</p>
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