675 lines
40 KiB
Text
675 lines
40 KiB
Text
|
||
<!DOCTYPE html>
|
||
<html lang="es" dir="ltr">
|
||
<head>
|
||
<meta charset="utf-8" />
|
||
<meta name="description" content="The Protein Structure and Function section of the NEI Laboratory of Retinal Cell and Molecular Biology studies cell differentiation survival and maintenance in the retina. Learn more about the lab and its research." />
|
||
<meta name="google" content="vityevxlt8UuVFWvgz9_YwmJ5F0Nby_B3t54LLh-3Zw,lACaj571Gx_VdLF3HKSKUf1Wxx0H_bWxO3BYDZ_C1uw" />
|
||
<meta name="google-site-verification" content="vityevxlt8UuVFWvgz9_YwmJ5F0Nby_B3t54LLh-3Zw" />
|
||
<meta name="google-site-verification" content="lACaj571Gx_VdLF3HKSKUf1Wxx0H_bWxO3BYDZ_C1uw" />
|
||
<meta name="google-site-verification" content="UDfyeEAWNX_rJUHwog9j1kziEnByGwhkSJGLJclUWDA" />
|
||
<meta name="viewport" content="width=device-width, initial-scale=1.0" />
|
||
<script type="application/ld+json">{
|
||
"@context": "https://schema.org",
|
||
"@graph": [
|
||
{
|
||
"@type": "WebPage",
|
||
"@id": "https://www.nei.nih.gov/research/research-labs-and-branches/protein-structure-and-function",
|
||
"breadcrumb": {
|
||
"@type": "BreadcrumbList",
|
||
"itemListElement": [
|
||
{
|
||
"@type": "ListItem",
|
||
"position": 1,
|
||
"name": "Home",
|
||
"item": "https://www.nei.nih.gov/espanol"
|
||
},
|
||
{
|
||
"@type": "ListItem",
|
||
"position": 2,
|
||
"name": "Protein Structure and Function",
|
||
"item": "https://www.nei.nih.gov/espanol/node/213"
|
||
}
|
||
]
|
||
},
|
||
"author": {
|
||
"@type": "GovernmentOrganization",
|
||
"@id": "https://www.nei.nih.gov",
|
||
"name": "National Eye Institute",
|
||
"url": "https://www.nei.nih.gov"
|
||
},
|
||
"publisher": {
|
||
"@type": "GovernmentOrganization",
|
||
"@id": "https://www.nei.nih.gov",
|
||
"name": "National Eye Institute",
|
||
"url": "https://www.nei.nih.gov"
|
||
}
|
||
}
|
||
]
|
||
}</script>
|
||
<link rel="icon" type="image/png" href="/themes/custom/nei/images/favicon.png" />
|
||
<link rel="apple-touch-icon" href="/themes/custom/nei/images/apple-touch-icon.png" />
|
||
<meta name="last-updated" content="Dec 17, 2024, 12:09:38 PM" />
|
||
<link rel="icon" href="/themes/custom/nei/favicon.ico" type="image/vnd.microsoft.icon" />
|
||
<link rel="alternate" hreflang="en" href="https://www.nei.nih.gov/research/research-labs-and-branches/protein-structure-and-function" />
|
||
<link rel="canonical" href="https://www.nei.nih.gov/research/research-labs-and-branches/protein-structure-and-function" />
|
||
<link rel="shortlink" href="https://www.nei.nih.gov/node/213" />
|
||
|
||
<title>Protein Structure and Function | National Eye Institute</title>
|
||
<link rel="preload" href="/themes/custom/nei/fonts/Aleo-Bold.woff2" as="font" type="font/woff2" crossorigin>
|
||
<link rel="preload" href="/themes/custom/nei/fonts/Lato-Regular.woff2" as="font" type="font/woff2" crossorigin>
|
||
<link rel="stylesheet" media="all" href="/sites/default/files/css/css_PGSk_7b88hq2qr_--_38MLvoj1ffr9jv3JhnNJTnKUM.css?delta=0&language=es&theme=nei&include=eJw1ytEKgCAMRuEXsnwk-dMxrOliTqi3j4iuDnycfFCprpbyHK4tDb-FRvwSshrFYvOErNhxBUHnCaYEcbJ_61Qji26Q5ZXa-QH_YCJ9" />
|
||
<link rel="stylesheet" media="all" href="/themes/custom/nei/css/style.css?sraguf" />
|
||
|
||
<script type="application/json" data-drupal-selector="drupal-settings-json">{"path":{"baseUrl":"\/","pathPrefix":"espanol\/","currentPath":"node\/213","currentPathIsAdmin":false,"isFront":false,"currentLanguage":"es"},"pluralDelimiter":"\u0003","gtag":{"tagId":"","consentMode":false,"otherIds":[],"events":[],"additionalConfigInfo":[]},"suppressDeprecationErrors":true,"ajaxPageState":{"libraries":"eJxdjFsOwjAMBC8U6iNFbmKZgPNQ7BTK6SmgCsrPrndkTbhSTFa7D0OtZq-2Cil8yoWOj5WYYT8c18pC3pCBt_jfE17wfoTZCRYeyORRjPruLmhpIS-b2EvFmArD73CFErDUGeWkoadmekCb5fXVsCN3bGeF2EdDmb5kGqWNWZKeKTpd1SjDjEpuSXRTeOeUaxxCT9WYZ50","theme":"nei","theme_token":null},"ajaxTrustedUrl":{"form_action_p_pvdeGsVG5zNF_XLGPTvYSKCf43t8qZYSwcfZl2uzM":true},"gtm":{"tagId":null,"settings":{"data_layer":"dataLayer","include_classes":false,"allowlist_classes":"google\nnonGooglePixels\nnonGoogleScripts\nnonGoogleIframes","blocklist_classes":"customScripts\ncustomPixels","include_environment":false,"environment_id":"","environment_token":""},"tagIds":["GTM-TLVNS7W"]},"user":{"uid":0,"permissionsHash":"c48594e108b5c1c2bea409821b71477d5bc2724c8cae6d31f1d985af0daab85c"}}</script>
|
||
<script src="/sites/default/files/js/js_WgzMsgqb-MiW-Z2aLffGQHepZwX2hWrEHSrb46WqZrk.js?scope=header&delta=0&language=es&theme=nei&include=eJxdjEEOgCAMBD-E9kmkYrOpqdQAMerr5eBBvUx29jCp8HUKQOkZAe4wiY1B6Pj7yAsf33MNmZvuEq0nojnPmkFvCVmUYD6xDTUV3Vq9ATUbLxk"></script>
|
||
<script src="https://script.crazyegg.com/pages/scripts/0117/6571.js" async></script>
|
||
<script src="/modules/contrib/google_tag/js/gtag.js?sraguf"></script>
|
||
<script src="/modules/contrib/google_tag/js/gtm.js?sraguf"></script>
|
||
|
||
</head>
|
||
<body class="t-lab_page t-node__213 path-node-213">
|
||
<a href="#main-content" class="u-skip-link">
|
||
Skip to content
|
||
</a>
|
||
<noscript><iframe src="https://www.googletagmanager.com/ns.html?id=GTM-TLVNS7W"
|
||
height="0" width="0" style="display:none;visibility:hidden"></iframe></noscript>
|
||
|
||
<div class="dialog-off-canvas-main-canvas" data-off-canvas-main-canvas>
|
||
|
||
<div id="u-page-outer" class="u-outer">
|
||
<div class="views-element-container site-notice-block" id="block-views-block-site-notices-block-1">
|
||
|
||
|
||
|
||
|
||
|
||
<div>
|
||
<div class="js-view-dom-id-0ad8bc2e426a8b1cafef8a2990c96d7d973a7a2e7a770918fcf24ec9352cfecc site-notices">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
|
||
|
||
</div>
|
||
|
||
|
||
<img src="/themes/custom/nei/images/NEI-logo-tagline.svg" alt="" width="200" height="48" class="u-print-only">
|
||
<div class="r-global-header">
|
||
<div id="r-header-outer" class="r-header">
|
||
|
||
<div class="r-header-inner">
|
||
|
||
<div id="block-nei-branding" class="o-block--logo" role="banner">
|
||
|
||
|
||
|
||
|
||
|
||
<div class="o-logo">
|
||
<a href="/espanol" class="o-logo__link" title="Home" rel="home"><span class="o-logo__text">National Eye Institute</span> <span class="o-logo__tagline">Research Today… Vision Tomorrow</span></a>
|
||
</div>
|
||
|
||
|
||
</div>
|
||
|
||
<div id="block-neimenubutton-2">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
<div class="o-menu-toggle"><button class="o-menu-toggle__btn" id="menu-toggle__btn" aria-controls="block-mainnavigation" aria-expanded="true">Menú</button></div>
|
||
|
||
|
||
|
||
|
||
</div>
|
||
<div class="language-switcher-language-url has-no-translation" id="block-languageswitcher" role="navigation" aria-label="Language switcher">
|
||
|
||
|
||
<ul class="links">
|
||
<li>
|
||
<a href="/research/research-labs-and-branches/protein-structure-and-function" class="language-link is-active" lang="en" data-drupal-link-system-path="node/213" aria-current="page">English</a>
|
||
</li>
|
||
<li class="is-active">
|
||
<a href="/espanol/node/213" class="language-link is-active" lang="es" data-drupal-link-system-path="node/213" aria-current="page"> Español</a>
|
||
</li>
|
||
</ul>
|
||
|
||
|
||
</div>
|
||
<div class="search-global c-search--global" data-drupal-selector="search-global" id="block-globalsearchform" role="search">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
<form action="/espanol/node/213" method="post" id="search-global" accept-charset="UTF-8">
|
||
<div class="c-form__inner">
|
||
<div class="js-form-item form-item js-form-type-textfield form-item-global-terms js-form-item-global-terms">
|
||
<label for="edit-global-terms">Buscar en la página</label>
|
||
<input data-drupal-selector="edit-global-terms" type="text" id="edit-global-terms" name="global-terms" value="" size="30" maxlength="128" placeholder="Buscar en la página" class="form-text" />
|
||
|
||
</div>
|
||
<input data-drupal-selector="form-ynylcni5lieedpxpwidgchjuembai2g9rid9v3hgzcu" type="hidden" name="form_build_id" value="form-YnYlcni5LIEEdpXpwidgCHjuembAi2g9rid9v3hgZCU" />
|
||
<input data-drupal-selector="edit-search-global" type="hidden" name="form_id" value="search_global" />
|
||
<div data-drupal-selector="edit-global-actions" class="form-actions js-form-wrapper form-wrapper" id="edit-global-actions"><input data-drupal-selector="edit-global-submit" type="submit" id="edit-global-submit" name="op" value="Buscar" class="button js-form-submit form-submit" />
|
||
</div>
|
||
|
||
</div>
|
||
</form>
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
|
||
</div>
|
||
|
||
</div>
|
||
|
||
<div class="r-menu-primary">
|
||
<nav aria-label="Main navigation" id="block-mainnavigation" class="o-menu-main__outer">
|
||
|
||
<ul class="o-menu o-menu-main"> <li class="o-menu-main__item o-menu-main__item--expanded o-menu-main__item--no-js"> <a href="/espanol/aprenda-sobre-la-salud-ocular" class="o-menu-main__link" data-drupal-link-system-path="node/39">Aprenda sobre la salud ocular</a>
|
||
<ul class="o-menu o-menu-main__submenu"> <li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/aprenda-sobre-la-salud-ocular/vision-saludable" class="o-menu-main__link" data-drupal-link-system-path="node/788">Visión saludable</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/aprenda-sobre-la-salud-ocular/enfermedades-y-afecciones-de-los-ojos" class="o-menu-main__link" data-drupal-link-system-path="learn-about-eye-health/eye-conditions-and-diseases">Enfermedades y afecciones de los ojos</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/aprenda-sobre-la-salud-ocular/datos-y-estadisticas-relacionadas-con-la-salud-ocular" class="o-menu-main__link" data-drupal-link-system-path="node/825">Datos y estadísticas relacionadas con la salud ocular</a>
|
||
</li>
|
||
<li class="o-menu-main__item"> <a href="/espanol/aprenda-sobre-la-salud-ocular/rehabilitacion-visual" class="o-menu-main__link" data-drupal-link-system-path="node/3865">Rehabilitación visual</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/aprenda-sobre-la-salud-ocular/campanas-de-difusion-y-recursos" class="o-menu-main__link" data-drupal-link-system-path="node/125">Campañas de difusión y recursos</a>
|
||
</li>
|
||
</ul>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--expanded o-menu-main__item--no-js"> <a href="/espanol/acerca-del-NEI" class="o-menu-main__link" data-drupal-link-system-path="node/275">Acerca del Instituto Nacional del Ojo</a>
|
||
<ul class="o-menu-main__submenu o-menu"> <li class="o-menu-main__item"> <a href="/espanol/acerca-del-NEI/planificacion-estrategica-en-el-nei" class="o-menu-main__link" data-drupal-link-system-path="node/2857">Planificación estratégica en el NEI</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/acerca-del-NEI/metas-y-logros" class="o-menu-main__link" data-drupal-link-system-path="node/281">Metas y logros</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/acerca-del-NEI/historia-del-nei" class="o-menu-main__link" data-drupal-link-system-path="node/282">Historia del NEI</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/acerca-del-NEI/lideres-del-instituto-nacional-del-ojo-nei" class="o-menu-main__link" data-drupal-link-system-path="node/415">Líderes del Instituto Nacional del Ojo (NEI)</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/acerca-del-NEI/el-presupuesto-y-el-congreso" class="o-menu-main__link" data-drupal-link-system-path="node/395">El presupuesto y el Congreso</a>
|
||
</li>
|
||
<li class="o-menu-main__item o-menu-main__item--collapsed"> <a href="/espanol/acerca-del-NEI/comites-asesores" class="o-menu-main__link" data-drupal-link-system-path="node/285">Comités asesores</a>
|
||
</li>
|
||
<li class="o-menu-main__item"> <a href="/espanol/aprenda-sobre-la-salud-ocular/campanas-de-difusion-y-recursos/programa-nacional-de-educacion-sobre-la-salud-del-ojo" title="National Eye Health Education Program" class="o-menu-main__link" data-drupal-link-system-path="node/265">Programa Nacional de Educación sobre la Salud del Ojo</a>
|
||
</li>
|
||
<li class="o-menu-main__item"> <a href="/espanol/acerca-del-NEI/done-al-instituto-nacional-del-ojo-nei" class="o-menu-main__link" data-drupal-link-system-path="node/286">Done al Instituto Nacional del Ojo (NEI)</a>
|
||
</li>
|
||
</ul>
|
||
</li>
|
||
</ul>
|
||
|
||
|
||
</nav>
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
<div class="o-overlay" aria-hidden="true"></div>
|
||
|
||
<div class="r-breadcrumb">
|
||
|
||
<div id="block-nei-breadcrumbs">
|
||
|
||
|
||
|
||
|
||
|
||
<nav aria-label="Breadcrumb">
|
||
<ol class="c-breadcrumb">
|
||
<li>
|
||
<a href="/espanol">Home</a>
|
||
</li>
|
||
<li>
|
||
Protein Structure and Function
|
||
</li>
|
||
</ol>
|
||
</nav>
|
||
|
||
|
||
|
||
</div>
|
||
|
||
|
||
</div>
|
||
|
||
|
||
|
||
<div class="r-page-header">
|
||
<div class="o-page-header">
|
||
|
||
<div id="block-nei-page-title">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
<h1>
|
||
<span>Protein Structure and Function</span>
|
||
</h1>
|
||
|
||
|
||
|
||
|
||
</div>
|
||
|
||
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
<div class="r-highlighted">
|
||
<div data-drupal-messages-fallback class="hidden"></div>
|
||
|
||
|
||
</div>
|
||
|
||
<div class="r-help">
|
||
|
||
</div>
|
||
<main id="main-content" class="r-main">
|
||
<div class="r-pre-content">
|
||
|
||
</div>
|
||
|
||
<div class="r-splitter">
|
||
<div class="r-content">
|
||
|
||
<div id="block-nei-content">
|
||
|
||
|
||
|
||
|
||
|
||
<article data-history-node-id="213" lang="en">
|
||
<div>
|
||
<div class="r-content">
|
||
|
||
|
||
<div class="c-section">
|
||
<h2>About our work</h2>
|
||
<div>
|
||
<div><div class="c-text">
|
||
|
||
|
||
|
||
|
||
<p>The interests of this section are in the area of protein structure as it relates to function, with a focus on the interactions of components involved in cell differentiation survival and maintenance. Our research at NEI has applied these interests to systems in the retina.</p>
|
||
|
||
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
|
||
|
||
<div class="c-section">
|
||
<h2>Current research</h2>
|
||
<div>
|
||
<div><div class="c-text">
|
||
|
||
|
||
|
||
|
||
<p>Our section has been studying pigment epithelium-derived factor (PEDF), a protein that acts in neuronal differentiation and survival in cells derived from the retina and CNS. PEDF inhibits angiogenesis and its expression is down- regulated over the replicative lifespan of mammals. This interesting factor is secreted by retinal pigment epithelial cells into the interphotoreceptor matrix, where it acts on photoreceptor cells. Its importance in the development, maintenance, and function of the retina and CNS is evident in animal models for inherited and light-induced retinal degeneration, as well as for degeneration of spinal cord motor neurons.</p><p>Much of our progress in understanding PEDF has relied on our development of overexpression systems that yielded recombinant proteins as functionally active neurotrophic factors identical to the native protein and ideal for biochemical, biophysical, and biological studies. The cDNA sequence for PEDF predicts a unique protein with strong homology to members of the serine protease inhibitor (serpin) superfamily. For this reason, we first studied PEDF as a serpin. We established that PEDF belongs with the subgroup of noninhibitory serpins and that it has characteristics of a substrate rather than an inhibitor of serine proteases. Moreover, a region from its amino-terminus confers the neurotrophic activity to the PEDF polypeptide, not requiring the serpin reactive loop (the structural determinant for serpin inhibition). We concluded that PEDF’s neurotrophic activity must be mediated by a mechanism independent of serine protease inhibition, and we proposed that during evolution this serpin might have lost its inhibitory activity and gained neurotrophic function. These findings provided an example of the separation of inhibitory and other activities in a serpin.</p><p>Our investigations were next directed toward the hypothesis that PEDF’s neurotrophic activity is mediated by interactions with cell surfaces. Focusing on retinoblastoma and cerebellar granule cells, we prospected for PEDF receptors and found evidence for 1) a saturable, specific, and high-affinity class of receptors on the surface of both cells, with characteristics of an 80-kDa plasma membrane protein, and 2) the amino-terminal region in PEDF that interacts with the receptor. Furthermore, using a yeast two-hybrid screening, we identified a novel gene from pigment epithelium of the human retina that codes for a PEDF-binding partner, which we term PEDF-R. We studied PEDF-R as a receptor for PEDF and found that the PEDF-R polypeptide has specific and high binding affinity for PEDF, has a potent phospholipase A(2) activity that liberates fatty acids, and is associated with eukaryotic cell membranes. Most importantly, PEDF binding stimulates the enzymatic phospholipase A(2) activity of PEDF-R. Our work, the first step in the biological activity of PEDF is the binding to receptors on the surface of target cells, i.e., PEDF-R, a phospholipase-linked membrane protein with high affinity for PEDF, suggests a molecular pathway by which ligand/receptor interaction on the cell surface could generate a cellular signal, a significant advance in the elucidation of PEDF’s mechanism of action.</p><p>We also investigated the association of PEDF with extracellular matrixes (ECM) and found that PEDF can interact with glycosaminoglycans and collagens. In the folded PEDF protein, the binding sites for these two ECM components are distinct and separated from each other, from the binding site for the receptor and from the serpin exposed loop. Our functional studies showed that glycosaminoglycans can act as positive modulators of the PEDF-receptor interactions. These findings are significant because the association of PEDF with ECMs may be used in vivo to regulate this protein, i.e., by spatial and temporal localization and/or controlling its activities.</p><p>We also studied the effects of matrix metalloproteinases (MMP) type-2 and type-9 on PEDF and demonstrated that they can fully degrade PEDF in a calcium dependent-fashion, abolishing its neurotrophic and antiangiogenic activities. Moreover, we found that hypoxia and VEGF can decrease PEDF protein levels by stimulating the MMP-mediated proteolytic degradation of PEDF. Interestingly, the expression and secretion of MMP-2/-9 and of VEGF correlate positively with the progression of neovascular ocular diseases, while the levels of PEDF correlate inversely. Most importantly, these results reveal a novel post-translational mechanism for downregulating PEDF by MMP-mediated proteolytic degradation that explains hypoxia-provoked increases in the VEGF/PEDF ratio, angiogenesis and/or neuronal death.</p><p>In preparation for protein therapeutics, we explored direct delivery of the PEDF protein to the choroid and retina. We demonstrated the permeability properties of the sclera and choroid/RPE to PEDF. Upon subconjunctival delivery, this relatively large protein traversed the sclera-choroid-RPE layers to reach the retina. Our conclusions suggest that subconjunctival routes of PEDF protein delivery offer novel, feasible and minimally invasive means for administration in the clinic.</p><p>We hope our PEDF research lays the groundwork for the development of therapies for diseases involving defective neuronal differentiation or cell survival, such as retinitis pigmentosa, age-related macular degeneration, and amyotrophic lateral sclerosis. The reported antiangiogenic effects of PEDF suggest it may also be useful in treating diseases in which new blood vessel formation plays a role, such as diabetic retinopathy, age-related macular degeneration, tumor growth, and rheumatoid arthritis.</p><p>The role of a cell-surface receptor in the mechanisms of action of PEDF represents a key aspect of regulation. Along these lines, our studies focus on structure-function relationships and enzymatic characterization of PEDF receptors, and their distribution in the normal and diseased retina. Consistent with our goal of elucidating the mechanisms of action of PEDF, we are interested in signal transduction and the expression of genes affected by PEDF’s stimuli. Given that protein therapeutics pose great challenges in development, production, delivery, safety, and stability, future work will be directed toward the molecular design of potent PEDF peptides for ocular treatment to achieve altered binding specificity and prevention of side effects, and the development of sustained delivery systems for PEDF in animal models of retinal and CNS diseases.</p>
|
||
|
||
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
|
||
|
||
<div class="c-section">
|
||
<h2>Selected publications</h2>
|
||
<div>
|
||
<div><div class="c-text">
|
||
|
||
|
||
|
||
|
||
<p>Samtani S, Amaral J, Campos MM, Fariss R, Becerra SP.: Doxycycline-mediated Inhibition of Choroidal Neovascularization. <em>Invest Ophthalmol Vis Sci.</em> 2009 Jun 10, 2009. <a href="http://www.ncbi.nlm.nih.gov/pubmed/19516001">PubMed</a></p><p>Becerra SP, Perez-Mediavilla LA, Weldon JE, Locatelli-Hoops S, Senanayake P, Notari L, Notario V, Hollyfield JG.: Pigment epithelium-derived factor binds to hyaluronan. Mapping of a hyaluronan binding site.<em> J Biol Chem.</em> 283(48):33310-20, 2008. Epub 2008 Sep 19. <a href="http://www.ncbi.nlm.nih.gov/pubmed/18805795">PubMed</a></p><p>Perruccio EM, Rowlette LL, Comes N, Locatelli-Hoops S, Notari L, Becerra SP, Borrs T.: Dexamethasone increases pigment epithelium-derived factor in perfused human eyes. <em>Curr Eye Res.</em> 33(5):507-15, 2008. <a href="http://www.ncbi.nlm.nih.gov/pubmed/18568889">PubMed</a></p><p>Lilja AE, Chang WL, Barry PA, Becerra SP, Shenk TE.: Functional genetic analysis of rhesus cytomegalovirus: Rh01 is an epithelial cell tropism factor. <em>J Virol.</em> 82(5):2170-81, 2008. Epub 2007 Dec 19. <a href="http://www.ncbi.nlm.nih.gov/pubmed/18094178">PubMed</a></p><p>Notari, L, Baladron, V., Aroca-Aguilar, J.D., Balko, N., Heredia, R., Meyer, C., Notario, P.M., Saravanamuthu, S., Nueda, M.L., Sanchez-Sanchez, F., Escribano, J., Laborda, J., Becerra, S.P.: Identification of a lipase-linked cell membrane receptor for pigment epithelium-derived factor. <em>J. Biol. Chem.</em> 281,38022-38037, 2006. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17032652&query_hl=1&itool=pubmed_docsum">PubMed</a></p><p>Amaral, J., Campos, M.M., Becerra, S.P., Fariss, R.N.: A novel imaging technique for experimental choroidal neovascularization. <em>Invest. Ophthalmol. Vis. Sci.</em> 47, 5163-5170, 2006. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17122098&query_hl=1&itool=pubmed_docsum">PubMed</a></p><p>Becerra, S.P.: Focus on Molecules: Pigment epithelium-derived factor (PEDF). <em>Exp. Eye Res. </em>82:739-40, 2006. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16364294&query_hl=1&itool=pubmed_docsum">PubMed</a></p><p>Amaral, J., Fariss, R.N, Campos,M.M., Robison, Jr,W.G., Kim,H., Lutz, R., Becerra, S.P.: Transscleral-RPE permeability of PEDF and ovalbumin proteins: implications for subconjunctival protein delivery. <em>Invest. Ophthalmol. Vis. Sci. </em>46, 4383-92, 2005. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16303924&query_hl=72&itool=pubmed_docsum">PubMed</a></p><p>Notari, L., Miller, A., Martnez, A., Amaral, J., Ju, M., Robinson, G., Smith, L.E.H., Becerra, S.P.: Pigment epithelium-derived factor is a substrate for matrix metalloproteinase type 2 and type 9: implications for downregulation in hypoxia. <em>Invest. Ophthalmol. Vis. Sci.</em> 46, 2736-2747, 2005. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16043845&query_hl=1&itool=pubmed_docsum">PubMed</a></p><p>Becerra, S.P., Fariss, R.N., Wu, Y.-Q., Montuenga, L., Wong, P., Pfeffer, B.A.: Pigment epithelium-derived factor in the monkey retinal pigment epithelium and interphotoreceptor matrix: apical secretion and distribution. Exp Eye Res. 78, 223-234, 2004. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=14729355&query_hl=1&itool=pubmed_docsum">PubMed</a></p><p>Alberdi, E.M., Weldon, J.E., Becerra, S.P.: Glycosaminoglycans in human retinoblastoma cells: Heparan sulfate, a modulator of the pigment epithelium-derived factor-receptor interactions. <em>BMC Biochemistry</em> 4:1, 2003 (19 February 2003) <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=12625842&query_hl=1&itool=pubmed_docsum">PubMed</a></p><p>Becerra, S.P., Amaral, J.: Erythropoietin–an endogenous retinal survival factor. <em>N. Engl. J. Med.</em> 347, 1968-1970, 2002. Review. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12477950&dopt=Abstract">PubMed</a></p><p>Meyer, C., Notari, L., and Becerra, S.P.: Mapping the type I collagen-binding site on pigment epithelium-derived factor. Implications for its antiangiogenic activity. <em>J. Biol Chem.</em> 277, 45400-45407, 2002. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12237317&dopt=Abstract">PubMed</a></p><p>Bilak, S.R., Bilak, M.M., Corse, A.M., Royal, W., Becerra, S.P., Kuncl, R.W.: Pigment Epithelium-Derived Factor is Elevated in CSF of Patients with ALS. <em>J. Neurochem.</em> 81, 178-184, 2002 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12067231&dopt=Abstract">PubMed</a></p><p>Gao, G., Li, Y., Fant, J., Crosson, C.E., Becerra, S.P., and Ma J.X.: Difference in ischemic regulation of vascular endothelial growth factor and pigment epithelium–derived factor in brown norway and sprague dawley rats contributing to different susceptibilities to retinal neovascularization. <em>Diabetes</em> 51, 1218-25, 2002 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11916948&dopt=Abstract">PubMed</a></p><p>Kuncl, R.W., Bilak, M.M., Bilak, S.R., Corse, A.M., Royal, W., Becerra, S.P.: Pigment epithelium-derived factor is elevated in CSF of patients with amyotrophic lateral sclerosis. <em>J. Neurochem.</em> 81, 178-184, 2002 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12067231&dopt=Abstract">PubMed</a></p><p>Aymerich, M.S., Alberdi, E., Martinez, A., and Becerra, S.P.: Evidence for pigment epithelium-derived factor (PEDF) receptors in the neural retina. <em>Invest. Ophthalmol. Vis. Sci.</em> 42, 3287-3293, 2001 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11726635&dopt=Abstract">PubMed</a></p><p>Nomura, T., Yabe, T. Mochizuki, H., Reiser, J., Becerra, S.P. and Schwartz, J.P.: Survival effects of pigment epithelium-derived factor (PEDF) expressed by a lentiviral vector in rat cerebellar granule cells. <em>Dev. Neurosci.</em> 23, 145-52, 2001 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11509837&dopt=Abstract">PubMed</a></p><p>Cayouette, M., Smith, S.B., Becerra, S.P., and Gravel, C.: Pigment epithelium-derived factor delays the death of photoreceptors in mouse models of inherited retinal degenerations <em>Neurobiol. Disease</em> 6, 523-532, 1999 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10600408&dopt=Abstract">PubMed</a></p><p>Alberdi, E., Aymerich, M.S., and Becerra, S.P.: Binding of pigment epithelium-derived factor (PEDF) to retinoblastoma and cerebellar granule cells: Evidence for a PEDF receptor <em>J. Biol. Chem.</em> 274, 31605-31612, 1999 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10531367&dopt=Abstract">PubMed</a></p><p>Houenou, L.J., D=Costa, A.P., Li, L., Turgeon, V.L., Enyadike, C., Alberdi, E., and Becerra, S.P.: Pigment epithelium-derived factor (PEDF) promotes the survival and differentiation of developing spinal motor neurons <em>J. Comparative Neurology</em> 412, 506-514, 1999. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10441236&dopt=Abstract">PubMed</a></p><p>Alberdi, E., Hyde, C.C., and Becerra, S.P.: Pigment epithelium-derived factor binds to glycosaminoglycans: Analysis of binding site. <em>Biochemistry</em> 37, 10643-10652, 1998 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9692954&dopt=Abstract">PubMed</a></p><p>Perez-Mediavilla, L.A., Chew, C., Campochiaro, P.A., Nickells, R.W., Notario, V., Zack, D.J. and Becerra, S.P. Sequence and expression analysis of bovine pigment epithelium-derived factor (PEDF). <em>Biochim. Biophys. Acta</em> 1398, 203-214, 1998 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9689919&dopt=Abstract">PubMed</a></p><p>Araki, T., Taniwaki, T., Becerra, S.P., Chader, G.J., and Schwartz, J.P.: Pigment epithelium-derived factor (PEDF) differentially protects immature but not mature cerebellar granule cells against apoptotic cell death. <em>J. Neurosci. Res.</em> 53, 7-15, 1998 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9670988&dopt=Abstract">PubMed</a></p><p>Sugita, Y., Becerra, S.P., Chader, G.J., and Schwartz, J.P.: Pigment epithelium-derived factor (PEDF) has direct effects on microglia and indirect effects on astrocytes in culture. <em>J. Neurosci. Res.</em> 49, 710-718, 1997 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9335258&dopt=Abstract">PubMed</a></p><p>Taniwaki, T., Hirashima, N., Becerra, S.P., Chader, G.J., Etcheberrigaray, R., and Schwartz, J.P.: Pigment epithelium-derived factor protects cultured cerebellar granule cells against glutamate-induce neurotoxicity. <em>J. Neurochem.</em> 68, 26-32, 1997 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=8978706&dopt=Abstract">PubMed</a></p><p>Becerra, S.P.: Structure-function relationships of PEDF: A noninhibitory serpin with neurotrophic activity. <em>Adv. Exp. Med. Biol.</em> 425, 223-237, 1997 <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9433504&dopt=Abstract">PubMed</a></p>
|
||
|
||
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
|
||
</div>
|
||
</div>
|
||
|
||
<div class="c-group__staff-list c-section" id="key-staff">
|
||
<h2>Protein Structure and Function key staff</h2>
|
||
<div class="views-element-container">
|
||
<div class="js-view-dom-id-780c4055fe93da3e17a7efc30884f3630de8f994d391cc544a48159cbcc3fca1 lab-staff">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
</div>
|
||
|
||
<div class="c-group__staff-list c-section" id="alumni-staff">
|
||
<div class="views-element-container">
|
||
<div class="js-view-dom-id-bf07da38585488b77ae6adcb7919f23804dcb50847f90a7de6c656b69ec4efdf lab-alumni">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
</div>
|
||
|
||
</article>
|
||
|
||
|
||
|
||
</div>
|
||
|
||
<div class="views-element-container" id="block-views-block-news-page-block-5">
|
||
|
||
|
||
|
||
|
||
|
||
<div>
|
||
|
||
<div class="c-filter js-view-dom-id-a96544a070eabac6147cc4a5e542413125136338080801e0c4a327de788e8e51 news-page news-page--block_5">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
|
||
|
||
</div>
|
||
|
||
|
||
|
||
<div id="block-researchatnei" class="c-callout c-callout--wide c-callout--rounded">
|
||
<figure class="c-callout__image">
|
||
<img src="/sites/default/files/styles/400px_square_crop/public/2019-04/shutterstock_605501567_333.jpg?itok=yofmpBZG" alt="">
|
||
</figure>
|
||
<div class="c-callout__content">
|
||
<h2 class="c-callout__heading">Research at NEI</h2>
|
||
|
||
|
||
|
||
|
||
<ul><li><a href="/research/research-labs-and-branches">Research Labs and Branches</a></li><li><a href="/research/research-labs-and-branches/we-are-nei-intramural">Principal Investigators</a></li><li><a href="/grants-and-training/training-nei">Training at NEI</a></li><li><a href="/about/news-and-events/news">Research News</a></li></ul>
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
<p class="views-element-container c-last-updated" id="block-views-block-nei-last-updated-block-1"><strong>Actualización más reciente:</strong> 17 de diciembre de 2024</p>
|
||
|
||
|
||
</div>
|
||
<aside class="r-sidebar" role="complementary">
|
||
<div class="c-sidebar-widget">
|
||
<div class="c-chief">
|
||
<div class="c-chief__upper">
|
||
<div class="views-element-container">
|
||
<div class="o-table--sortable js-view-dom-id-685f98e485589d005e59224915a170d8b8e25ef8fe79f992d642b865e70c6b5c lab-staff">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
</div>
|
||
</div>
|
||
<div class="c-sidebar-widget__contact-information">
|
||
<div class="c-sidebar-widget__header">
|
||
<h2 class="c-sidebar-widget__title">Contact <span>Information</span></h2>
|
||
</div>
|
||
<div class="views-element-container">
|
||
<div class="js-view-dom-id-b14495cc676706c2c280eb184e580e231f25c045c51aa768dfb8aef17b3f3aa4 sidebar-address-labs">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
<div>
|
||
<div class="c-sidebar-widget__contact-name">
|
||
|
||
<span>Protein Structure and Function</span>
|
||
|
||
</div>
|
||
|
||
<div class="c-sidebar-widget__contact-address">
|
||
6 Center Drive MSC 0608<br>
|
||
<br>
|
||
Bethesda,
|
||
Maryland
|
||
20892-0608
|
||
</div>
|
||
|
||
<div class="c-sidebar-widget__contact-phone-fax">
|
||
|
||
|
||
<div>
|
||
<div><span>Phone:</span> 301-496-6514</div>
|
||
</div>
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
</div>
|
||
|
||
<div class="c-sidebar-widget__contact-additional-info">
|
||
<a href="#key-staff" class="c-link__link">See additional lab contact information</a>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</aside>
|
||
</div>
|
||
</main>
|
||
|
||
<div class="r-footer">
|
||
<footer class="o-footer" role="contentinfo">
|
||
|
||
<div id="block-footer-2">
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
|
||
<div class="o-footer__row">
|
||
<div class="o-footer__group o-footer__group--logo"><a class="o-logo__link" href="/" rel="home" title="Home"><span class="o-logo__text">National Eye Institute</span></a>
|
||
<ul class="o-menu-social">
|
||
<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--facebook outlink-ignore" href="https://www.facebook.com/NationalEyeInstitute">Facebook</a></li>
|
||
<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--twitter outlink-ignore" href="https://twitter.com/NatEyeInstitute">Twitter</a></li>
|
||
<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--linkedin outlink-ignore" href="https://www.linkedin.com/company/national-eye-institute-nei">LinkedIn</a></li>
|
||
<li class="o-menu-social__item"><a class="o-menu-social__link o-menu-social__link--youtube outlink-ignore" href="https://www.youtube.com/user/neinih">YouTube</a></li>
|
||
</ul>
|
||
|
||
<p> </p>
|
||
</div>
|
||
|
||
<div class="o-footer__group o-footer__group--contact">
|
||
<h2 class="o-footer__title">Contactenos NEI</h2>
|
||
|
||
<ul class="o-menu-footer">
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/contactenos">Contáctenos</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.nih.gov/about-nih/visitor-information">Visite a los NIH (en inglés)</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://list.nih.gov/cgi-bin/wa.exe?SUBED1=insidenei&A=1">Reciba actualizaciones del NEI a través del correo electrónico (en inglés)</a></li>
|
||
</ul>
|
||
</div>
|
||
|
||
<div class="o-footer__group o-footer__group--policies">
|
||
<h2 class="o-footer__title">Politicas</h2>
|
||
|
||
<ul class="o-menu-footer">
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/informacion-sobre-accesibilidad">Accesibilidad</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.nih.gov/nondiscrimination-notice-language-assistance-services#spanish">Información en otros idiomas</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/ley-de-libertad-de-informacion-foia">Política de privacidad</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/ley-de-libertad-de-informacion-foia">Ley de Libertad de Información (FOIA)</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/descargo-de-responsabilidades">Descargo de responsabilidades</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="/espanol/politicas-de-la-pagina-web">Políticas de la página web</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.hhs.gov/vulnerability-disclosure-policy/index.html ">Declaración sobre vulnerabilidad (en inglés)</a></li>
|
||
</ul>
|
||
</div>
|
||
|
||
<div class="o-footer__group o-footer__group--employees">
|
||
<h2 class="o-footer__title">Para los empleados de NEI</h2>
|
||
|
||
<ul class="o-menu-footer">
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://intranet.nei.nih.gov">Intranet</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://intranet.nei.nih.gov/hr/Pages/emergency.aspx">Información de emergencia</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://ned.nih.gov/search/search.aspx">Directorio de los funcionarios del NEI</a></li>
|
||
</ul>
|
||
</div>
|
||
</div>
|
||
|
||
<div class="o-footer__row">
|
||
<div class="o-footer__full o-footer__full--parent">
|
||
<ul class="o-menu-footer o-menu-footer--swing">
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://www.nih.gov/">NIH.gov</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://hhs.gov/">HHS.gov</a></li>
|
||
<li class="o-menu-footer__item"><a class="o-menu-footer__link" href="https://usa.gov/">USA.gov</a></li>
|
||
</ul>
|
||
</div>
|
||
</div>
|
||
|
||
|
||
|
||
|
||
|
||
</div>
|
||
|
||
|
||
</footer>
|
||
</div>
|
||
|
||
</div>
|
||
|
||
</div>
|
||
|
||
|
||
|
||
<div class="c-lb-wrapper" id="lb-wrapper" aria-hidden="true">
|
||
<div class="c-lb-overlay" tabindex="-1"></div>
|
||
<div role="dialog" class="c-lb" id="lb">
|
||
<div class="c-lb-header">
|
||
<button class="c-lb-close-btn"><span class="c-lb-close-btn-text">Close</span><span class="c-lb-close-btn-text times" aria-hidden="true">×</span></button>
|
||
</div>
|
||
<figure class="c-lb-figure">
|
||
<figcaption class="c-lb-caption"></figcaption>
|
||
</figure>
|
||
</div>
|
||
</div>
|
||
|
||
<script src="/sites/default/files/js/js_P5UBl7ML5jj6dp4P_zp1vfnURYj58JsfQMLHLuwiELA.js?scope=footer&delta=0&language=es&theme=nei&include=eJxdjEEOgCAMBD-E9kmkYrOpqdQAMerr5eBBvUx29jCp8HUKQOkZAe4wiY1B6Pj7yAsf33MNmZvuEq0nojnPmkFvCVmUYD6xDTUV3Vq9ATUbLxk"></script>
|
||
<script src="/themes/custom/nei/js/scripts.js?sraguf"></script>
|
||
|
||
</body>
|
||
</html>
|