32 lines
1.5 KiB
HTML
32 lines
1.5 KiB
HTML
<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
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<html>
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<head>
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<meta name="referrer" content="origin-when-cross-origin" />
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<meta name="ncbi_app" content="static" />
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<meta name="ncbi_pdid" content="primerblast_info" />
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<script type="text/javascript"> var ncbi_startTime = new Date(); </script>
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<title></title>
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</head>
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<body>
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<h1></h1>
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<pre>
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Primer-BLAST was developed at NCBI to help users make primers that are specific
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to intended PCR target. It uses <a href="https://github.com/primer3-org/primer3">Primer3</a> (version 2.5.0) to design PCR primers and then uses
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BLAST and global alignment algorithm to screen primers against user-selected database
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in order to avoid primer pairs (all combinations including forward-reverse primer pair,
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forward-forward as well as reverse-reverse pairs) that can cause non-specific amplifications.
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To cite Primer-BLAST or look for more details, please consult our publication:
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<a href="https://www.ncbi.nlm.nih.gov/pubmed/?term=22708584">Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden T (2012).
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Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction.
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BMC Bioinformatics. 13:134.
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</a>
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Use of Primer3 itself is subject to the terms and conditions stated on <a href="https://primer3.ut.ee/">Primer3 web site</a>.
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</pre>
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<script type="text/javascript" src="/portal/portal3rc.fcgi/rlib/js/InstrumentOmnitureBaseJS/InstrumentNCBIBaseJS/InstrumentPageStarterJS.js"></script>
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</body>
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</html>
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