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<a title="SRA Submission Quick Start" href="/sra/docs/submit">Submission Quick Start</a></li>
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<a title="Archive Documentation" href="/sra/docs/sra-data-storage-model/">Archive Documentation</a>
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<div class="container">
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<div id="maincontent" class="content col twelve_col last">
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<div class="col1">
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<h1 id="submit-your-project-and-biologic">Submit your project and biological samples</h1>
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<div class="jig-ncbiinpagenav" data-all-heading-levels="["h2"]" data-top-of-page-t-o-c="true">
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<h2 id="introduction">Introduction</h2>
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<div class="warning-message"><img src="/core/assets/sra/images/tack.png" alt="Tack" height="20" /> Registering project and biological samples at the NCBI BioProject and
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the BioSample databases is a prerequisite for any public SRA submission. The BioProject and BioSample databases store data
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that relate to organizational and biological aspects of sequencing experiments.
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</div>
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<p><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" /> If you are making your submission
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via dbGaP or GEO databases please refer to our <a target="_blank" title="Submit for dbGaP project" href="/sra/docs/submitdbgap">dbGaP submission Guide</a>
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and <a target="_blank" title="Submit for GEO project" href="/sra/docs/submitgeo">GEO submission Guide</a> respectively.</p>
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<p><img src="/core/assets/sra/images/galka.png" alt="Check" /> You can complete both your BioProject as well as BioSample
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submissions (including batch submissions of biological samples)
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within <a target="_blank" title="SRA Submission Portal" href="https://submit.ncbi.nlm.nih.gov/subs/sra/">the SRA Submission Portal Wizard</a>.</p>
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<div class="shadowing"><img src="/core/assets/sra/images/SRA-submission-wizard-for-bio.png" alt="SRA submission wizards with BioProject and BioSamples" /></div>
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<p><strong>BioProject submission</strong> describes the goal of the sequencing study, whereas <strong>BioSample submission</strong>
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records the nature of the biological material that had been sequenced.</p>
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<div class="shadowing"><a data-jig="ncbipopper" data-resizable="true" href="#bio"><img src="/core/assets/sra/images/anatomy_of_BioProject_BioSample_submission.png" alt="Anatomy of BioProject and BioSample submission" width="500" /></a></div>
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<div class="shadowing" id="bio"><img src="/core/assets/sra/images/anatomy_of_BioProject_BioSample_submission.png" alt="Anatomy of BioProject and BioSample submission" width="1000" /></div>
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<h2 id="bioproject-submissions-guideline">BioProject submissions guidelines</h2>
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<h3 id="quick-guide">Quick guide</h3>
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<p><span class="strong">BioProject</span> describes the goal of your research effort. In course of a single study, you may accumulate data that
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are suitable for submission in multiple NCBI databases. Therefore, you don't have to create a project exclusively
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for your SRA submission. To be eligible for SRA submission the project (1) must exist; (2) should not be an
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umbrella project; (3) should not be a RefSeq project. SRA does not have restrictions on the project's data type.</p>
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<ul>
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<li>Click on the link <strong>BioProject</strong> on <a target="_blank" title="Submission Portal Home Page, new window" href="https://submit.ncbi.nlm.nih.gov/">Submission Portal Home page</a> or <a target="_blank" title="Submission Portal BioProject, new window" href="https://submit.ncbi.nlm.nih.gov/subs/bioproject/">log in here</a>.</li>
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<li>Click on the button <span class="greenbutton">New submission</span>.</li>
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<li>Browser-based wizard will help you to create your BioProject submission. In the section <strong>Target</strong> usually either <strong>Organism name</strong> or <strong>Description</strong> are mandatory depending on the <strong>Sample scope</strong> you provided in <strong>Project type</strong> section. You don't have to provide BioSample accession(s) or register your sample(s) within the BioProject submission wizard.</li>
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<li>After finalizing your submission by clicking the <span class="greenbutton">Submit</span> button, view all your BioProject submissions and their status at <https: submit.ncbi.nlm.nih.gov="" subs="" bioproject=""></https:></li>
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||
</ul>
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||
<div class="shadowing"><img src="/core/assets/sra/images/view_bioproject.png" alt="My BioProject submissions" width="600" /></div>
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<p><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" /> Use BioProject accession (<strong><code>PRJNA#</code></strong>) for your SRA submission.</p>
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<p><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" /> In the SRA Submission Portal Wizard, registration
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||
of BioProject is simplified and BioProject accession (<strong><code>PRJNA#</code></strong>) is not required for SRA metadata and assigned after the SRA submission is finished.</p>
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<p><img src="/core/assets/sra/images/tack.png" alt="Tack" height="20" /> Registered project becomes <span class="strong">STUDY</span> in SRA submission and receives SRA accession in the form <strong><code>SRP#</code></strong>. Use the SRP# accession in your publications for referencing your SRA submissions.</p>
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<h3 id="bioproject-links">BioProject links</h3>
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<ul>
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<li><a target="_blank" title="BioProject Database, new window" href="/bioproject/">The NCBI BioProject database</a></li>
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<li><a target="_blank" title="BioProject, new window" href="/bioproject/docs/faq/">BioProject Frequently Asked Questions</a></li>
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</ul>
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<h2 id="biosample-submissions-guidelines">BioSample submissions guidelines</h2>
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<h3 id="quick-guide_1">Quick guide</h3>
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<p><span class="strong">BioSample</span> is a record of biological isolate with unique physical properties.
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Biological and technical replicates (in most cases) should not be considered unique BioSamples.</p>
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<p>Examples:</p>
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<ul>
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<li>23,000 unique 16S amplicons from a single seawater collection point – 1 BioSample (1 sample was collected and then analyzed to deduce 16S diversity)</li>
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<li>3 "identical" transgenic mice treated with the same drug as part of an experiment – 1 BioSample</li>
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<li>CHO cells infected with a virus and sampled at 0, 2, 4, and 8 hours post infection – 4 BioSamples (4 time points)</li>
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<li>RNA-Seq data from a single male anteater taken from the brain, heart, lungs, testes, and liver – 5 BioSamples (5 different tissues isolated)</li>
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</ul>
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<p><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" /> Majority of SRA submissions require more
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than one sample. Make sure that every organism/metagenome, time point, tissue type or treatment type have their own sample. </p>
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<p><img src="/core/assets/sra/images/tack.png" alt="Tack" height="20" /> Select most appropriate taxonomy for
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organism/metagenome in <a target="_blank" title="Taxonomy browser" href="/taxonomy/">the NCBI Taxonomy browser</a>.
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Providing the exact and most specific name for the organism/metagenome will simplify your BioSample submission. </p>
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<p><img src="/core/assets/sra/images/tack.png" alt="Tack" height="20" /> For metagenome or environmental samples look up appropriate
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taxonomy in the NCBI Taxonomy browser <a target="_blank" title="unspecified sequences and metagenomes, new window" href="/Taxonomy/Browser/wwwtax.cgi?mode=Undef&id=12908">Unspecified sequences</a> taxonomy.</p>
|
||
<p>For more information refer to <a target="_blank" title="BioSample documentation" href="/biosample/docs/">BioSample documentation</a>.</p>
|
||
<h3 id="register-samples">Register samples</h3>
|
||
<ul>
|
||
<li>Click on the link <strong>BioSample</strong> on <a target="_blank" title="Submission Portal Home Page, new window" href="https://submit.ncbi.nlm.nih.gov/">Submission Portal Home page</a> or <a target="_blank" title="Submission Portal BioSample, new window" href="https://submit.ncbi.nlm.nih.gov/subs/biosample/">log in here</a></li>
|
||
<li>Click on the button <span class="greenbutton">New submission</span>.</li>
|
||
<li>
|
||
<p>Browser-based wizard will help you to create your BioSample submission:</p>
|
||
<ul>
|
||
<li>In <strong>Submitter</strong> section: check your contact information and affiliation;</li>
|
||
<li>In <strong>General information</strong> section: (1) indicate release date; (2) specify whether you want to create one or multiple samples (please note that for SRA submission multiple samples are more common);</li>
|
||
<li>In <strong>Sample type</strong> section: select most appropriate sample type;</li>
|
||
<li>
|
||
<p>In <strong>Attributes</strong> section: download Excel template, fill it out, convert into tab-delimited text file, and upload the text file by clicking the button <span class="greenbutton">Browse</span> and selecting the file on your computer;</p>
|
||
<p><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" /> Each sample name must be meaningful and UNIQUE WITHIN YOUR ACCOUNT. Please do not name your samples by names of your sequence data files.</p>
|
||
</li>
|
||
<li>
|
||
<p>In <strong>Comment</strong> section: provide comment (optional);</p>
|
||
</li>
|
||
<li>In <strong>Overview</strong> section: revise your submission and click the button <span class="greenbutton">Submit</span>.</li>
|
||
</ul>
|
||
</li>
|
||
</ul>
|
||
<p>After finalizing your submission by clicking the button <span class="greenbutton">Submit</span>, view all your BioSample submissions and their status at <https: submit.ncbi.nlm.nih.gov="" subs="" biosample=""></https:></p>
|
||
<div class="shadowing"><img src="/core/assets/sra/images/view_biosample.png" alt="Your BioSample accessions in Submission Portal" width="600" /></div>
|
||
<h3 id="retrieve-biosample-accessions">Retrieve BioSample accessions</h3>
|
||
<ul>
|
||
<li>Click on the link <strong><code>SUB#</code></strong> to view BioSample submission's overview;</li>
|
||
<li>Click on <strong><code>Successfully loaded ([number] objects)</code></strong>.</li>
|
||
</ul>
|
||
<p><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" /> Use the BioSample accessions (<strong><code>SAMN#</code></strong>) in your SRA submission. </p>
|
||
<p><img src="/core/assets/sra/images/tack.png" alt="Tack" height="20" /> Registered sample becomes <span class="strong">SAMPLE</span> in SRA submission and receives SRA accession in the form <strong><code>SRS#</code></strong>.</p>
|
||
<h3 id="biosample-links">BioSample links</h3>
|
||
<ul>
|
||
<li><a target="_blank" title="BioSample database, new window" href="/biosample/">The NCBI BioSample database</a> </li>
|
||
<li><a target="_blank" title="BioSample documentation, new window" href="/biosample/docs/">BioSample documentation</a></li>
|
||
</ul>
|
||
<h2 id="updating-bioproject-and-biosampl">Updating BioProject and BioSample data</h2>
|
||
<p><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" /> Regardless of whether you created your project and samples in the SRA Submission Wizard or separately in the BioProject or BioSample databases,
|
||
contact <a href="mailto:bioprojecthelp@ncbi.nlm.nih.gov">bioprojecthelp@ncbi.nlm.nih.gov</a> or <a href="mailto:biosamplehelp@ncbi.nlm.nih.gov">biosamplehelp@ncbi.nlm.nih.gov</a> for assistance in updating your BioProject or BioSample
|
||
submission respectively. </p>
|
||
<p><span class="warning-message"><img src="/core/assets/sra/images/znak.png" alt="Exclamation point" height="20" />
|
||
When you change <a target="_blank" title="SRA Change release date" href="/sra/docs/srachangereleasedate">the release date</a> of your SRA data in the SRA UI, the release date of your project and samples changes accordingly.</span> </p>
|
||
<hr />
|
||
<h2 id="contact-sra-staff">Contact SRA staff</h2>
|
||
<p>Please email <a href="mailto:sra@ncbi.nlm.nih.gov">sra@ncbi.nlm.nih.gov</a> </p>
|
||
</div>
|
||
|
||
|
||
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|
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<!--/.col1-->
|
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<div class="col2">
|
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<div id="shared-content-1">
|
||
<h2 data-heading="h2" data-no-toc="true">Getting Started</h2>
|
||
<ul>
|
||
<li><a title="SRA Getting Started" href="/sra/docs/">Getting Started</a></li>
|
||
</ul>
|
||
<h2 data-heading="h2" data-no-toc="true">Submitting Data to SRA</h2>
|
||
<h3 class="nav" data-heading="h3" data-no-toc="true">General</h3>
|
||
<ul>
|
||
<li><a title="SRA Submission Quick Start" href="/sra/docs/submit">Quick Start</a></li>
|
||
<li><a title="How to submit BioProject and BioSample for SRA" href="/sra/docs/submitbio">BioProject & BioSample</a></li>
|
||
<li><a title="SRA file format guide" href="/sra/docs/submitformats">File Format Guide</a></li>
|
||
<li><a title="Understanding SRA Metadata and Submission Overview" href="/sra/docs/submitmeta">SRA Metadata Overview</a></li>
|
||
<li><a title="SRA File Upload" href="/sra/docs/submitfiles">SRA File Upload</a></li>
|
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|
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|
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|
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|
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<li><a title="Submitting Data in Submission Portal" href="/sra/docs/submitportal">Submitting to SRA</a></li>
|
||
<li><a title="Troubleshooting SRA submission" href="/sra/docs/submitspfiles">Troubleshooting Submission</a></li>
|
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</ul>
|
||
<h3 class="nav" data-heading="h3" data-no-toc="true">Submitting for dbGaP & GEO</h3>
|
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<ul>
|
||
<li><a title="Submitting SRA data for dbGaP project" href="/sra/docs/submitdbgap">Submitting for dbGaP</a></li>
|
||
<li><a title="Submitting SRA data for GEO" href="/sra/docs/submitgeo">Submitting for GEO</a></li>
|
||
</ul>
|
||
<h3 class="nav" data-heading="h3" data-no-toc="true">Updating SRA Data</h3>
|
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<ul>
|
||
<li><a title="Updating SRA submission" href="/sra/docs/submitupdate">Updating SRA data</a> </li>
|
||
<li><a title="Request status change" href="/sra/docs/request-status-change">Request status change</a> </li>
|
||
<li><a title="Change release date" href="/sra/docs/submitsra">Change Release Date</a></li>
|
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|
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|
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<p class="address_footer text-white">National Library of Medicine<br />
|
||
<a href="https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/@38.9959508,-77.101021,17z/data=!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb:0x19156f88b27635b8!8m2!3d38.9959508!4d-77.0988323" class="text-white" target="_blank" rel="noopener noreferrer">8600 Rockville Pike<br />
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Bethesda, MD 20894</a></p>
|
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<div class="col-lg-3 col-12 centered-lg">
|
||
<p><a href="https://www.nlm.nih.gov/web_policies.html" class="text-white">Web Policies</a><br />
|
||
<a href="https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office" class="text-white">FOIA</a><br />
|
||
<a href="https://www.hhs.gov/vulnerability-disclosure-policy/index.html" class="text-white" id="vdp">HHS Vulnerability Disclosure</a></p>
|
||
</div>
|
||
<div class="col-lg-3 col-12 centered-lg">
|
||
<p><a class="supportLink text-white" href="https://support.nlm.nih.gov/">Help</a><br />
|
||
<a href="https://www.nlm.nih.gov/accessibility.html" class="text-white">Accessibility</a><br />
|
||
<a href="https://www.nlm.nih.gov/careers/careers.html" class="text-white">Careers</a></p>
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||
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|
||
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<div class="col-lg-12 centered-lg">
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<nav class="bottom-links">
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<li>
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||
<a class="text-white" href="//www.nlm.nih.gov/">NLM</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.nih.gov/">NIH</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.hhs.gov/">HHS</a>
|
||
</li>
|
||
<li>
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<a class="text-white" href="https://www.usa.gov/">USA.gov</a>
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</li>
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</ul>
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</nav>
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<p class="last-updated small">Last updated: 2019-09-20T18:22:57Z</p>
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</div>
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<span class="PAFAppResources"></span>
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