732 lines
45 KiB
HTML
732 lines
45 KiB
HTML
<?xml version="1.0" encoding="utf-8"?>
|
||
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
|
||
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
|
||
|
||
<head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
|
||
<!-- AppResources meta begin -->
|
||
<meta name="paf-app-resources" content="" />
|
||
<!-- AppResources meta end -->
|
||
|
||
<!-- TemplateResources meta begin -->
|
||
<meta name="paf_template" content="StdNCol" />
|
||
|
||
<!-- TemplateResources meta end -->
|
||
|
||
<!-- Page meta begin -->
|
||
|
||
<!-- Page meta end -->
|
||
|
||
<!-- Logger begin -->
|
||
<meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_app" content="gtr" /><meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_pdid" content="static" />
|
||
<!-- Logger end -->
|
||
|
||
<title>How to submit information in the Methodology section of the submission form</title>
|
||
|
||
<!-- PageFixtures headcontent begin -->
|
||
<meta content="gtr" name="ncbi_db" /><meta content="index, follow, noarchive" name="robots" /><meta content="IE=edge,chrome=1" http-equiv="X-UA-Compatible" />
|
||
|
||
<meta name="cms-local-nav-url" content="https://cms.ncbi.nlm.nih.gov///gtr/help_docs_nav" />
|
||
|
||
<!-- PageFixtures headcontent end -->
|
||
|
||
<!-- AppResources external_resources begin -->
|
||
<script type="text/javascript" src="/core/jig/1.15.6/js/jig.min.js"></script>
|
||
|
||
<!-- AppResources external_resources end -->
|
||
|
||
<!-- Page headcontent begin -->
|
||
<meta name="subsite" content="gtr" />
|
||
<meta name="path" content="gtr/docs/submit/test_method" />
|
||
<meta name="modified" content="2020-12-02T23:21:12Z" />
|
||
<link type="text/css" rel="stylesheet" href="/core/assets/gtr/css/feedback_css.css" />
|
||
<link type="text/css" rel="stylesheet" href="/core/assets/gtr/css/gtr_cms.css" />
|
||
<link type="text/css" rel="stylesheet" href="/core/assets/gtr/css/main.css" />
|
||
<!-- Page headcontent end -->
|
||
<!-- PageFixtures resources begin -->
|
||
<link xmlns="http://www.w3.org/1999/xhtml" type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218191/css/4207974/4206132.css" xml:base="http://127.0.0.1/sites/static/header_footer" />
|
||
|
||
<!-- PageFixtures resources end -->
|
||
<link rel="shortcut icon" href="//www.ncbi.nlm.nih.gov/favicon.ico" /><meta name="ncbi_phid" content="CE8CBE627C8BE321000000000025001D.m_7" />
|
||
<meta name='referrer' content='origin-when-cross-origin'/><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4221774/css/4121862/3974050/3917732/251717/4171260/14534/45193/3534283/4128070/4003311/4005757/4110101/4173824.css" /><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4221774/css/3529741.css" media="print" /></head>
|
||
<body class=" static">
|
||
<div class="grid">
|
||
<div class="col twelve_col nomargin shadow">
|
||
<!-- System messages like service outage or JS required; this is handled by the TemplateResources portlet -->
|
||
<div class="sysmessages">
|
||
<noscript>
|
||
<p class="nojs">
|
||
<strong>Warning:</strong>
|
||
The NCBI web site requires JavaScript to function.
|
||
<a href="/guide/browsers/#enablejs" title="Learn how to enable JavaScript" target="_blank">more...</a>
|
||
</p>
|
||
</noscript>
|
||
</div>
|
||
<!--/.sysmessage-->
|
||
<div class="wrap">
|
||
<div class="page">
|
||
<div xmlns:xi="http://www.w3.org/2001/XInclude">
|
||
<div xmlns="http://www.w3.org/1999/xhtml" id="universal_header" xml:base="http://127.0.0.1/sites/static/header_footer">
|
||
<section class="usa-banner">
|
||
<div class="usa-accordion">
|
||
<header class="usa-banner-header">
|
||
<div class="usa-grid usa-banner-inner">
|
||
<img src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/favicons/favicon-57.png" alt="U.S. flag" />
|
||
<p>An official website of the United States government</p>
|
||
<button class="non-usa-accordion-button usa-banner-button" aria-expanded="false" aria-controls="gov-banner-top" type="button">
|
||
<span class="usa-banner-button-text">Here's how you know</span>
|
||
</button>
|
||
</div>
|
||
</header>
|
||
<div class="usa-banner-content usa-grid usa-accordion-content" id="gov-banner-top" aria-hidden="true">
|
||
<div class="usa-banner-guidance-gov usa-width-one-half">
|
||
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-dot-gov.svg" alt="Dot gov" />
|
||
<div class="usa-media_block-body">
|
||
<p>
|
||
<strong>The .gov means it's official.</strong>
|
||
<br />
|
||
Federal government websites often end in .gov or .mil. Before
|
||
sharing sensitive information, make sure you're on a federal
|
||
government site.
|
||
</p>
|
||
</div>
|
||
</div>
|
||
<div class="usa-banner-guidance-ssl usa-width-one-half">
|
||
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-https.svg" alt="Https" />
|
||
<div class="usa-media_block-body">
|
||
<p>
|
||
<strong>The site is secure.</strong>
|
||
<br />
|
||
The <strong>https://</strong> ensures that you are connecting to the
|
||
official website and that any information you provide is encrypted
|
||
and transmitted securely.
|
||
</p>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</section>
|
||
<div class="usa-overlay"></div>
|
||
<header class="ncbi-header" role="banner" data-section="Header">
|
||
|
||
<div class="usa-grid">
|
||
<div class="usa-width-one-whole">
|
||
|
||
<div class="ncbi-header__logo">
|
||
<a href="/" class="logo" aria-label="NCBI Logo" data-ga-action="click_image" data-ga-label="NIH NLM Logo">
|
||
<img src="https://www.ncbi.nlm.nih.gov/coreutils/nwds/img/logos/AgencyLogo.svg" alt="NIH NLM Logo" />
|
||
</a>
|
||
</div>
|
||
|
||
<div class="ncbi-header__account">
|
||
<a id="account_login" href="https://account.ncbi.nlm.nih.gov" class="usa-button header-button" style="display:none" data-ga-action="open_menu" data-ga-label="account_menu">Log in</a>
|
||
<button id="account_info" class="header-button" style="display:none" aria-controls="account_popup" type="button">
|
||
<span class="fa fa-user" aria-hidden="true">
|
||
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24" width="20px" height="20px">
|
||
<g style="fill: #fff">
|
||
<ellipse cx="12" cy="8" rx="5" ry="6"></ellipse>
|
||
<path d="M21.8,19.1c-0.9-1.8-2.6-3.3-4.8-4.2c-0.6-0.2-1.3-0.2-1.8,0.1c-1,0.6-2,0.9-3.2,0.9s-2.2-0.3-3.2-0.9 C8.3,14.8,7.6,14.7,7,15c-2.2,0.9-3.9,2.4-4.8,4.2C1.5,20.5,2.6,22,4.1,22h15.8C21.4,22,22.5,20.5,21.8,19.1z"></path>
|
||
</g>
|
||
</svg>
|
||
</span>
|
||
<span class="username desktop-only" aria-hidden="true" id="uname_short"></span>
|
||
<span class="sr-only">Show account info</span>
|
||
</button>
|
||
</div>
|
||
|
||
<div class="ncbi-popup-anchor">
|
||
<div class="ncbi-popup account-popup" id="account_popup" aria-hidden="true">
|
||
<div class="ncbi-popup-head">
|
||
<button class="ncbi-close-button" data-ga-action="close_menu" data-ga-label="account_menu" type="button">
|
||
<span class="fa fa-times">
|
||
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 48 48" width="24px" height="24px">
|
||
<path d="M38 12.83l-2.83-2.83-11.17 11.17-11.17-11.17-2.83 2.83 11.17 11.17-11.17 11.17 2.83 2.83 11.17-11.17 11.17 11.17 2.83-2.83-11.17-11.17z"></path>
|
||
</svg>
|
||
</span>
|
||
<span class="usa-sr-only">Close</span></button>
|
||
<h4>Account</h4>
|
||
</div>
|
||
<div class="account-user-info">
|
||
Logged in as:<br />
|
||
<b><span class="username" id="uname_long">username</span></b>
|
||
</div>
|
||
<div class="account-links">
|
||
<ul class="usa-unstyled-list">
|
||
<li><a id="account_myncbi" href="/myncbi/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_myncbi">Dashboard</a></li>
|
||
<li><a id="account_pubs" href="/myncbi/collections/bibliography/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_pubs">Publications</a></li>
|
||
<li><a id="account_settings" href="/account/settings/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_settings">Account settings</a></li>
|
||
<li><a id="account_logout" href="/account/signout/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_logout">Log out</a></li>
|
||
</ul>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
|
||
</div>
|
||
</div>
|
||
</header>
|
||
<div role="navigation" aria-label="access keys">
|
||
<a id="nws_header_accesskey_0" href="https://www.ncbi.nlm.nih.gov/guide/browsers/#ncbi_accesskeys" class="usa-sr-only" accesskey="0" tabindex="-1">Access keys</a>
|
||
<a id="nws_header_accesskey_1" href="https://www.ncbi.nlm.nih.gov" class="usa-sr-only" accesskey="1" tabindex="-1">NCBI Homepage</a>
|
||
<a id="nws_header_accesskey_2" href="/myncbi/" class="set-base-url usa-sr-only" accesskey="2" tabindex="-1">MyNCBI Homepage</a>
|
||
<a id="nws_header_accesskey_3" href="#maincontent" class="usa-sr-only" accesskey="3" tabindex="-1">Main Content</a>
|
||
<a id="nws_header_accesskey_4" href="#" class="usa-sr-only" accesskey="4" tabindex="-1">Main Navigation</a>
|
||
</div>
|
||
<section data-section="Alerts">
|
||
<div class="ncbi-alerts-placeholder"></div>
|
||
</section>
|
||
</div>
|
||
</div>
|
||
<!--/.header-->
|
||
<div class="header">
|
||
|
||
<div class="gtr_logo"><a href="/gtr" title="Genetic Testing Registry">NIH Genetic Testing Registry</a></div>
|
||
<div><div class="header_con2"><div id="searchBox" class="smallSearchBox"><form id="formSearch" data-selected-scope="docs" action="https://www.ncbi.nlm.nih.gov/gtr/" method="get"><div class="formFields noWrap"><label for="gtr_term" class="ui-helper-hidden-accessible">Search term</label><input id="gtr_term" name="term" type="text" data-jig="ncbiautocomplete" value="all[sb]" data-jigconfig="maxListLimit: 15" data-dictionary="gtr_allgtr" /><select name="scope" class="scope_sel" id="scope"><option value="gtr">All GTR</option><option value="conditions">Conditions/Phenotypes</option><option value="genes">Genes</option><option value="tests">Human Tests</option><option value="testsmicrobe">Microbe Tests</option><option value="labs">Labs</option></select><button type="submit" id="submitSearchButton" class="gtr-button-submit ui-ncbibutton-blue">Search</button><a href="/gtr/tests/advanced/" id="go-to-advanced-search">Advanced search for tests</a></div></form></div></div></div>
|
||
|
||
|
||
|
||
</div>
|
||
<div class="nav_and_browser">
|
||
<div class="localnav"><ul class="jig-ncbilocalnav">
|
||
<li><a href="#">Overview</a><ul>
|
||
<li><a href="/gtr/docs/about/">About GTR</a></li>
|
||
<li><a title="Submission overview" href="/gtr/docs/submit/">Submission to GTR</a></li>
|
||
<li><a title="Resources included in GTR and where to locate a genetics professional" href="/gtr/docs/resources/">GTR resources</a></li>
|
||
<li><a title="Frequently asked questions" href="/gtr/docs/faq/">FAQ</a></li>
|
||
<li><a href="https://www.nih.gov/institutes-nih/nih-office-director">NIH Office of the Director</a></li>
|
||
<li><a href="/gtr/docs/outreach/">Community outreach</a></li>
|
||
<li><a href="/gtr/docs/maintenance_use/">Data use and maintenance</a></li>
|
||
<li><a href="/clinvar/nomenclature/">Terminology</a></li>
|
||
<li><a href="/gtr/docs/linkcriteria/">Web link criteria</a></li>
|
||
<li><a href="/gtr/docs/linking/">How to create links to GTR</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Lab Submission</a><ul>
|
||
<li><a href="/gtr/docs/login/">Login</a></li>
|
||
<li><a title="Overview of how to submit novel laboratory information" href="/gtr/docs/submit/add_lab/">Register a new lab</a></li>
|
||
<li><a title="Instructions for performing the annual review" href="/gtr/docs/annual_review/">Annual review</a></li>
|
||
<li><a title="Instructions for managing the group account for submitters" href="/gtr/docs/manage_permissions/">Groups: Manage person permissions</a></li>
|
||
<li><a title="Instructions for submitters about updating and deleting records" href="/gtr/docs/manage_records/">Manage records</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Genetic Test Submission</a><ul>
|
||
<li><a title="Overview of genetic test submissions" href="/gtr/docs/submit/">Getting started</a></li>
|
||
<li><a title="Instructions for clinical genetic test submissions" href="/gtr/docs/submit/add_test/">Clinical tests</a></li>
|
||
<li><a title="Instructions for spreadsheet submissions for clinical genetic tests" href="/gtr/docs/fulltest/">Clinical tests by spreadsheet</a></li>
|
||
<li><a title="Instructions for API submissions for clinical genetic tests" href="/gtr/docs/gtr_api_http/">Clinical tests by api</a></li>
|
||
<li><a title="Instructions for research genetic test submissions" href="/gtr/docs/submit/add_research/">Research tests</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Microbe Test Submission</a><ul>
|
||
<li><a title="Overview of microbe test submissions" href="/gtr/docs/microbe_test/">Getting started</a></li>
|
||
<li><a title="Instructions for clinical microbe test submissions" href="/gtr/docs/submit/add_microbe_clinical/">Clinical tests</a></li>
|
||
<li><a title="Instructions for spreadsheet submissions for clinical microbe tests" href="/gtr/docs/microbe_fulltest/">Clinical tests by spreadsheet</a></li>
|
||
<li><a title="Instructions for API submissions for clinical microbe tests" href="/gtr/docs/gtr_api_http/">Clinical tests by api</a></li>
|
||
<li><a title="Instructions for research microbe test submissions" href="/gtr/docs/submit/add_microbe_research/">Research tests</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Search GTR</a><ul>
|
||
<li><a title="How to use and search the GTR website" href="/gtr/docs/help/">Search tips</a></li>
|
||
<li><a href="/gtr/docs/advanced_search">Advanced search</a></li>
|
||
<li><a href="/gtr/docs/common_searches/">Common searches</a></li>
|
||
<li><a href="https://www.youtube.com/playlist?list=PL7dF9e2qSW0a1b37gVee-pYFZ4AEOGvqw">YouTube library</a>
|
||
<span id="shared-content-1"></span></li>
|
||
</ul>
|
||
</li>
|
||
</ul></div>
|
||
</div>
|
||
|
||
<!-- was itemctrl -->
|
||
<div class="container">
|
||
<div id="maincontent" class="content col twelve_col last">
|
||
<div class="col1">
|
||
<div><div id="static-page-tools"><div style="display:inline-block;"><form id="form-helpdocs" action="/gtr/docs/" method="get"><label class="ui-helper-hidden-accessible" for="helpdocs-search-term">Search GTR Help Documents</label><input autocomplete="off" value="" placeholder="Search GTR Help Documents" name="term" id="helpdocs-search-term" style="width:30em;" /><button id="search">Search GTR help documents</button></form></div><ul><li class="print"><a href="#" id="print-this" title="Print this document">Print this document</a></li><li class="print"><a href="/gtr/docs/submit/test_submission">View and print all Test Submission documents</a></li></ul></div>
|
||
|
||
|
||
<h2 id="how-to-submit-information-in-the">How to submit information in the Methodology section of the submission form</h2>
|
||
|
||
|
||
<h3 id="strategy">Strategy</h3>
|
||
|
||
|
||
<p>To make your test more discoverable, we strongly recommend being as specific as possible in specifying what your test measures (Test targets - genes, chromosomes, proteins and analytes). For example, rather than providing free text about all the alleles or all the analytes being tested, submit each separately in the test targets section. Then use the Test comment section to describe how the combination of the specific targets is evaluated. GTR users will then be able to find your test by searching on specific genes, variants, analytes, etc. and NCBI resources such as ClinVar and MedGen will make more effective linkages to your submitted information.</p>
|
||
|
||
|
||
<h3 id="organization-of-this-page">Organization of this page</h3>
|
||
|
||
|
||
<p>This page is organized according to the sections you see on the Methodology tab. You may click on the name of the section below to navigate to that section of this document.</p>
|
||
|
||
|
||
<p>Please note that most sections in the form provide tool tips, accessible by this icon (<img src="/core/icons/help.png" alt="help icon" />), that provide hints specific to that area.</p>
|
||
|
||
|
||
<ul>
|
||
<li><a href="#methods">Test Method(s)</a></li>
|
||
<li><a href="#targets">Test Targets</a></li>
|
||
</ul>
|
||
|
||
|
||
<h3 id="methods">Test Method(s)</h3>
|
||
|
||
|
||
<p>This section organizes specific fields into blocks to capture the hierarchies among methodologies and to enable the description of complex tests. Major method category, Method category, Primary Test Methodology and Instruments are grouped and numbered. You may 'add another method' if more than one group is desired or you may remove a section. Information in the Test Methods section will display in the Methodology tab of the test record.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/TestMethodBlock.PNG" alt="Test method section" /></p>
|
||
|
||
|
||
<p><strong>Major method category</strong> (minimal). Choose a higher level method category from the list: Biochemical, Cytogenetic, or Molecular.</p>
|
||
|
||
|
||
<p><strong>Method category</strong> (minimal). Select the general category to which the method belongs.</p>
|
||
|
||
|
||
<p>Choices within Biochemical:
|
||
<em>Analyte<br />
|
||
Enzyme assay<br />
|
||
Immunohistochemistry<br />
|
||
Protein analysis<br />
|
||
Protein expression</em> </p>
|
||
|
||
|
||
<p>Choices within Cytogenetics:<br />
|
||
<em>FISH-metaphase<br />
|
||
FISH-interphase<br />
|
||
Chromosome breakage studies<br />
|
||
Sister chromatid exchange<br />
|
||
Chromosome Rearrangement<br />
|
||
Multicolor FISH (M-FISH)/Spectral Karyotyping™ (SKY™)</em></p>
|
||
|
||
|
||
<p>Choices within Molecular:<br />
|
||
<em>Deletion/duplication analysis<br />
|
||
Detection of homozygosity<br />
|
||
Linkage analysis<br />
|
||
Methylation analysis<br />
|
||
Microsatellite instability testing (MSI)<br />
|
||
Mutation scanning of select exons<br />
|
||
Mutation scanning of the entire coding region<br />
|
||
Sequence analysis of select exons<br />
|
||
Sequence analysis of the entire coding region<br />
|
||
Targeted mutation analysis<br />
|
||
Uniparental disomy study (UPD)</em></p>
|
||
|
||
|
||
|
||
<p><strong>Primary Test Methodology</strong> (minimal). Provide name of the test method used in the assay e.g., RT-PCR with gel analysis, Metabolite levels, or specify a method not listed.</p>
|
||
|
||
|
||
|
||
<p><strong>Instruments</strong> (recommended). Name the instrument used for a specific methodology e.g., Roche LightCycler 480, IlluminaHiSeq™2000 system, or specify an instrument not listed. Field is optional for research tests.</p>
|
||
|
||
|
||
|
||
<p><strong>Platforms</strong> (optional). Select the name of a platform containing pre-defined test targets to provide methodology details and help GTR users find your test. If you use a platform not on the list, choose ‘Other’ and then provide the name. A platform name typically corresponds to a unique catalogue number or a set of catalogue numbers which are bundled with other consumables. The format is usually manufacturer name, product name, and general specifications. Field is optional for research tests.</p>
|
||
|
||
|
||
|
||
<p><strong>Test procedure or protocol</strong> (optional). Summarize the methodology and describe the specific steps for each method of the assay.</p>
|
||
|
||
|
||
<p><strong>Citations for test procedure or protocol</strong> (optional). Provide citations or URL for the protocol.</p>
|
||
|
||
|
||
<p><strong>Confirmation of test results</strong> (recommended). Describe whether and how test results are confirmed (beyond initial validation of the assay). For example, 'Positive results are confirmed on a new DNA preparation using repeat sequence analysis'.<span> </span></p>
|
||
|
||
|
||
<p>NOTE about research tests: Field is optional. State whether or not participants have an opportunity to learn about the results of the research test. If so, describe how participants may find out the test results, whether confirmation may be done in a clinical (e.g. CLIA-certified) lab, and whether or not there may be associated costs.</p>
|
||
|
||
|
||
<p><strong>Test comment(s)</strong> (optional). Provide textual description of the test methods or any other information about your test not described elsewhere that may be useful to clinicians ordering your test, e.g. 'Bi-directional sequencing of exons 1-5 with concurrent analysis of NP_000000.0:p.Glu234Gly'.</p>
|
||
|
||
|
||
<p>NOTE about research tests: Use the Test comment(s) field to describe whether or not there a cost to participate in the study and if so, provide details.</p>
|
||
|
||
|
||
<p>NOTE we strongly encourage you to describe the test target(s) your test interrogates in the "Test Targets" section. For the example above, you would follow these steps:</p>
|
||
|
||
|
||
<ol>
|
||
<li>select 'gene' from the drop down menu for 'target is identified by',</li>
|
||
<li>start typing the gene symbol and select the correct gene from the autocomplete dictionary,</li>
|
||
<li>click the radio button for 'Associated reference sequences and exons',</li>
|
||
<li>start typing the RefSeq accession and select the correct Refseq from the autocomplete dictionary</li>
|
||
<li>enter the exons</li>
|
||
<li>click the radio button for Variants</li>
|
||
<li>start typing the HGVS expression for a tested variation, beginning with the RefSeq accession (ex. NM or NP),</li>
|
||
<li>select one of the suggestions from the autocomplete dictionary or enter NM_000208.2:c.3079C>T</li>
|
||
<li>click on 'Save target' to save your data</li>
|
||
</ol>
|
||
|
||
|
||
<p>By being specific, rather than writing free text, you make it possible for us to link your test to other resources at NCBI, such as variaton-related reports and ClinVar. This will ensure your test is more readily discoverable by all users of NCBI databases.</p>
|
||
|
||
|
||
<h3 id="targets">Test Targets</h3>
|
||
|
||
|
||
<p>Section to describe the analytes, chromosomal or mitochondrial regions, genes and variations, or proteins that are measured in the test. Use the 'Target is associated with' section to match each target with at least one condition. The test must have at least one explicit condition-target relationship.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/Add-test-target.PNG" alt="Add test target" /></p>
|
||
|
||
|
||
<p>Note the warning about the need to add at least one test target for every test, and to have at least one explicit condition-target relationship. Click ‘Add Test Target’ to open that section of the Methodology page.</p>
|
||
|
||
|
||
<p>Use the Test target section to enter targets (genes must be entered one at a time). Decide which specific target you are going to enter. For each target, you can provide:</p>
|
||
|
||
|
||
<ul>
|
||
<li>the type (germline, somatic or both) in ‘Target is’ section</li>
|
||
<li>the associated condition(s) that this target evaluates in ‘Target is associated with’ section</li>
|
||
<li>category (analyte, chromosomal region/mitochondrion, gene, protein) in ‘Target is identified by’ section</li>
|
||
</ul>
|
||
|
||
|
||
<p>As an example, below are the steps to enter JAG1 for Alagille syndrome 1 and NOTCH2 for Alagille syndrome 2.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/Add-test-target-2.PNG" alt="Add test target page" /></p>
|
||
|
||
|
||
<p><strong>Target is</strong> (recommended). Select a radio button to indicate the type of target: germline, somatic, or both. Please note that germline is selected by default.</p>
|
||
|
||
|
||
<p><strong>Target is associated with</strong> (recommended). Check one or more boxes for the indication(s) that are evaluated by the test target. In this case, Alagille syndrome 1 is associated with the JAG1 gene and Alagille syndrome 2 is associated with the NOTCH2 gene. For this example, check the Alagille syndrome 1 box.</p>
|
||
|
||
|
||
<p><strong>Target is identified by</strong> (minimal). Choose 'analyte', 'chromosomal region/mitochondrion', 'gene' or 'protein'. Then specify target(s) tested in field that appears below it (which is dependent on the selection made). In this example, for ‘Target is identified by’ choose ‘gene’.</p>
|
||
|
||
|
||
<p><strong>Gene: please specify</strong> (minimal if 'gene' is selected in 'Target is identified by' field). Begin typing in the box to activate autocomplete dictionary and select the desired term. Then select radio button for 'Associated Reference Sequences and Exons', 'Variants', or 'None'. The first two radio buttons activate fields to provide additional details. In this example, specify the gene in the pop-up window by typing JAG1 and selecting your choice.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/Add-gene.png" alt="Add a gene" /></p>
|
||
|
||
|
||
<p><strong>Associated Reference Sequence</strong> (minimal if 'Associated Reference Sequence and Exons' selected, otherwise optional). Activate autocomplete dictionary by typing NG or NM or NC to specify the accession and version (e.g. <span>NM</span>_0000001.1) of the sequence used to define the test or test region. Note, NC is applicable only to genes on the mitochondrion.</p>
|
||
|
||
|
||
<p>NOTE: If gene name is provided, the chromosomal location will be automatically provided and does not need to be entered.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/AssociatedReferenceSequencesAndExons.PNG" alt="How to enter reference sequences and exons" /></p>
|
||
|
||
|
||
<p><strong>Relevant exons for each associated reference sequence</strong> (minimal if 'Associated Reference Sequence and Exons' selected, otherwise optional). We encourage the following conventions for reporting the exons being tested:</p>
|
||
|
||
|
||
<p><table border="1" cellpadding="1" cellspacing="1">
|
||
<thead>
|
||
<tr>
|
||
<th scope="col">What is tested</th>
|
||
<th scope="col">How to report</th>
|
||
</tr>
|
||
</thead>
|
||
<tbody>
|
||
<tr>
|
||
<td>If a continuous range is tested</td>
|
||
<td>
|
||
<p>Provide the exon numbers as a range, <em>e.g.</em> 2-5.</p>
|
||
<p>If the range includes all exons, add (All), <em>e.g.</em> 1-5 (All)</p>
|
||
</td>
|
||
</tr>
|
||
<tr>
|
||
<td>If a subset is tested</td>
|
||
<td>Enumerate the exons tested. Ranges are allowed, <em>e.g.</em> 3,5,9-12</td>
|
||
</tr>
|
||
</tbody>
|
||
</table>
|
||
<strong>Relevant gene variant</strong> (minimal if 'Variants' selected). Activate autocomplete dictionary by typing NG, NM or NP and select the desired variant if available. Specify each mutation being tested using the HGVS expression (preferred), or the allele name.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/VariantsTestTarget.PNG" alt="How to enter variants" /></p>
|
||
|
||
|
||
<p><strong>Clinical significance of variant</strong> (recommended if 'Variants' selected). Options are:
|
||
<em>association<br />
|
||
confers sensitivity<br />
|
||
drug response<br />
|
||
no known pathogenicity<br />
|
||
not provided<br />
|
||
other<br />
|
||
pathogenic<br />
|
||
probably not pathogenic<br />
|
||
probably pathogenic<br />
|
||
risk factor<br />
|
||
variant of unknown significance</em></p>
|
||
|
||
|
||
|
||
<p><strong>Citations to support the clinical significance</strong> (recommended). Provide citations or URL.</p>
|
||
|
||
|
||
<p>Then ‘Save target’. Your have now completed a target entry (the JAG1 gene) for Alagille syndrome 1.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/Methodology-tab-One-gene-saved.PNG" alt="One gene saved" /></p>
|
||
|
||
|
||
<p>Complete your work by clicking ‘Add Test Target’ again in order to specify the NOTCH2 gene for Alagille syndrome 2. Specify whether the target is germline, somatic or both. In this example, uncheck Alagille syndrome 1 and check Alagille syndrome 2 to prepare for entering the gene NOTCH2. For ‘Target is identified by’ choose ‘gene’, then specify in the pop-up window by typing NOTCH2 and selecting your choice. Save Target.</p>
|
||
|
||
|
||
<p><strong>Analyte: please specify</strong> (minimal if 'analyte' is selected in 'Target is identified by' field). Begin typing in box to activate autocomplete dictionary, then select desired term. For example, type 'br' and select the analyte 'Branched-chain amino acids' or the enzyme 'Branched-chain ketoacid dehydrogenase'. Use 'Add another analyte' link to add multiple analytes.</p>
|
||
|
||
|
||
<p><img src="/core/assets/gtr/images/AnalyteTestTarget.PNG" alt="How to enter analyte class" /></p>
|
||
|
||
|
||
<p><strong>Chromosomal region/mitochondrion: please specify</strong> (optional; minimal if gene name or analyte not provided). Identify chromosome or chromosome location as precisely as possible. If individual chromosomes are evaluated, enter each one as a distinct target. Use the term 'Complete genome' for comprehensive chromosomal or genomic assessments such as karyotyping, cytogenomic arrays, whole genome sequencing or chromosome breakage analysis.</p>
|
||
|
||
|
||
<p><em>When identifying a chromosome,</em> begin typing in box 'Chromosome...' to activate autocomplete dictionary and select the desired term from the list that displays. If the desired chromosome is not listed, enter 'Chromosome [number]' in the text box and click the Save button at the bottom of the page.</p>
|
||
|
||
|
||
<p><em>When identifying a chromosomal region or band,</em> begin typing to activate autocomplete dictionary and select the desired term from the list that displays. For example, enter chromosomal region '11p15' or range '15q11-q13'. If the desired chromosomal region is not listed, enter it in the text box. Click the Save button at the bottom of the page.</p>
|
||
|
||
|
||
<p>GTR uses the same formatting conventions for chromosomal band as HGNC, OMIM and NCBI's Gene. When identifying a cytogenetic band, please represent it in the p-q order and do not repeat the chromosome number. Examples: 22q11.3-q11.4 or 5p15.33-p.15.2.</p>
|
||
|
||
|
||
<p><em>When your test target is the complete mitochondrial genome,</em> begin typing in box 'mitochondrion'. If the condition is known to result from variants in the mitochondrion, then the choice 'mitochondrion' will appear in the list that displays. (If the condition is not stored in GTR's database as being mitochondrial in origin, 'mitochondrion' will not display so you should enter 'mitochondrion' explicitly.)</p>
|
||
|
||
|
||
<p>NOTE: If you are testing specific mitochondrial genes, please select 'gene' in the Target is identified by section and select the genes by entering the beginning part of the gene symbol, namely MT-.</p>
|
||
|
||
|
||
<p>If gene name is provided, the chromosomal location will be automatically provided and does not need to be entered.</p>
|
||
|
||
|
||
<p><strong>Protein: please specify</strong> (minimal if 'protein' is selected in 'Target is identified by' field). Begin typing in box to activate autocomplete dictionary and select the desired term. Add another protein as needed.</p>
|
||
|
||
|
||
<h3 id="save-your-work">Save your work</h3>
|
||
|
||
|
||
<p><strong>Don't forget to save your work</strong> by clicking the Save & Continue button at the bottom of the page! If you need to interrupt or delay data entry, you can return at a later time and you will be taken to the last tab you saved.</p>
|
||
|
||
|
||
<p>If any required fields or data inconsistencies are detected on this page, an error message will display after you click Save & Continue indicating what the issue is. The field(s) that need attention will be outlined in red. Please correct your data and click "Save & Continue" again.</p>
|
||
|
||
|
||
<p><span id="shared-content-1"></span></p></div>
|
||
|
||
</div>
|
||
<!--/.col1-->
|
||
<div class="col2">
|
||
|
||
</div>
|
||
<!--/.col2-->
|
||
<div class="col3">
|
||
|
||
</div>
|
||
<!--/.col3-->
|
||
<div class="col4">
|
||
|
||
</div>
|
||
<!--/.col4-->
|
||
<div class="col5">
|
||
|
||
</div>
|
||
<div class="col6">
|
||
|
||
</div>
|
||
<div class="col7">
|
||
|
||
</div>
|
||
<div class="col8">
|
||
|
||
</div>
|
||
<div class="col9">
|
||
|
||
</div>
|
||
</div><!--/.content-->
|
||
</div><!--/.container-->
|
||
<div id="NCBIFooter_dynamic">
|
||
<!--<component id="NCBIBreadcrumbs"/>
|
||
<component id="NCBIHelpDesk"/>-->
|
||
<noscript><img alt="" src="/stat?jsdisabled=true&ncbi_app=gtr&ncbi_db=&ncbi_pdid=static&ncbi_phid=CE8CBE627C8BE321000000000025001D" /></noscript>
|
||
</div>
|
||
|
||
|
||
<div xmlns:xi="http://www.w3.org/2001/XInclude">
|
||
<div xmlns="http://www.w3.org/1999/xhtml" class="footer" id="footer" xml:base="http://127.0.0.1/sites/static/header_footer">
|
||
<section class="icon-section">
|
||
<div id="icon-section-header" class="icon-section_header">Follow NCBI</div>
|
||
<div class="grid-container container">
|
||
<div class="icon-section_container">
|
||
<a class="footer-icon" id="footer_twitter" href="https://twitter.com/ncbi" aria-label="Twitter">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="40" height="40" viewBox="0 0 40 40" fill="none">
|
||
<title>Twitter</title>
|
||
<g id="twitterx1008">
|
||
<path id="path1008" d="M6.06736 7L16.8778 20.8991L6.00001 32.2H10.2L18.6 23.1L25.668 32.2H34L22.8 17.5L31.9 7H28.4L20.7 15.4L14.401 7H6.06898H6.06736ZM9.66753 8.73423H12.9327L29.7327 30.4658H26.5697L9.66753 8.73423Z" fill="#5B616B"></path>
|
||
</g>
|
||
</svg>
|
||
</a>
|
||
<a class="footer-icon" id="footer_facebook" href="https://www.facebook.com/ncbi.nlm" aria-label="Facebook"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
||
<title>Facebook</title>
|
||
<path class="cls-11" d="M210.5,115.12H171.74V97.82c0-8.14,5.39-10,9.19-10h27.14V52l-39.32-.12c-35.66,0-42.42,26.68-42.42,43.77v19.48H99.09v36.32h27.24v109h45.41v-109h35Z">
|
||
</path>
|
||
</svg></a>
|
||
<a class="footer-icon" id="footer_linkedin" href="https://www.linkedin.com/company/ncbinlm" aria-label="LinkedIn"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
||
<title>LinkedIn</title>
|
||
<path class="cls-11" d="M101.64,243.37H57.79v-114h43.85Zm-22-131.54h-.26c-13.25,0-21.82-10.36-21.82-21.76,0-11.65,8.84-21.15,22.33-21.15S101.7,78.72,102,90.38C102,101.77,93.4,111.83,79.63,111.83Zm100.93,52.61A17.54,17.54,0,0,0,163,182v61.39H119.18s.51-105.23,0-114H163v13a54.33,54.33,0,0,1,34.54-12.66c26,0,44.39,18.8,44.39,55.29v58.35H198.1V182A17.54,17.54,0,0,0,180.56,164.44Z">
|
||
</path>
|
||
</svg></a>
|
||
<a class="footer-icon" id="footer_github" href="https://github.com/ncbi" aria-label="GitHub"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
||
<defs>
|
||
<style>
|
||
.cls-11,
|
||
.cls-12 {
|
||
fill: #737373;
|
||
}
|
||
|
||
.cls-11 {
|
||
fill-rule: evenodd;
|
||
}
|
||
</style>
|
||
</defs>
|
||
<title>GitHub</title>
|
||
<path class="cls-11" d="M151.36,47.28a105.76,105.76,0,0,0-33.43,206.1c5.28,1,7.22-2.3,7.22-5.09,0-2.52-.09-10.85-.14-19.69-29.42,6.4-35.63-12.48-35.63-12.48-4.81-12.22-11.74-15.47-11.74-15.47-9.59-6.56.73-6.43.73-6.43,10.61.75,16.21,10.9,16.21,10.9,9.43,16.17,24.73,11.49,30.77,8.79,1-6.83,3.69-11.5,6.71-14.14C108.57,197.1,83.88,188,83.88,147.51a40.92,40.92,0,0,1,10.9-28.39c-1.1-2.66-4.72-13.42,1-28,0,0,8.88-2.84,29.09,10.84a100.26,100.26,0,0,1,53,0C198,88.3,206.9,91.14,206.9,91.14c5.76,14.56,2.14,25.32,1,28a40.87,40.87,0,0,1,10.89,28.39c0,40.62-24.74,49.56-48.29,52.18,3.79,3.28,7.17,9.71,7.17,19.58,0,14.15-.12,25.54-.12,29,0,2.82,1.9,6.11,7.26,5.07A105.76,105.76,0,0,0,151.36,47.28Z">
|
||
</path>
|
||
<path class="cls-12" d="M85.66,199.12c-.23.52-1.06.68-1.81.32s-1.2-1.06-.95-1.59,1.06-.69,1.82-.33,1.21,1.07.94,1.6Zm-1.3-1">
|
||
</path>
|
||
<path class="cls-12" d="M90,203.89c-.51.47-1.49.25-2.16-.49a1.61,1.61,0,0,1-.31-2.19c.52-.47,1.47-.25,2.17.49s.82,1.72.3,2.19Zm-1-1.08">
|
||
</path>
|
||
<path class="cls-12" d="M94.12,210c-.65.46-1.71,0-2.37-.91s-.64-2.07,0-2.52,1.7,0,2.36.89.65,2.08,0,2.54Zm0,0"></path>
|
||
<path class="cls-12" d="M99.83,215.87c-.58.64-1.82.47-2.72-.41s-1.18-2.06-.6-2.7,1.83-.46,2.74.41,1.2,2.07.58,2.7Zm0,0">
|
||
</path>
|
||
<path class="cls-12" d="M107.71,219.29c-.26.82-1.45,1.2-2.64.85s-2-1.34-1.74-2.17,1.44-1.23,2.65-.85,2,1.32,1.73,2.17Zm0,0">
|
||
</path>
|
||
<path class="cls-12" d="M116.36,219.92c0,.87-1,1.59-2.24,1.61s-2.29-.68-2.3-1.54,1-1.59,2.26-1.61,2.28.67,2.28,1.54Zm0,0">
|
||
</path>
|
||
<path class="cls-12" d="M124.42,218.55c.15.85-.73,1.72-2,1.95s-2.37-.3-2.52-1.14.73-1.75,2-2,2.37.29,2.53,1.16Zm0,0"></path>
|
||
</svg></a>
|
||
<a class="footer-icon" id="footer_blog" href="https://ncbiinsights.ncbi.nlm.nih.gov/" aria-label="Blog">
|
||
<svg xmlns="http://www.w3.org/2000/svg" id="Layer_1" data-name="Layer 1" viewBox="0 0 40 40">
|
||
<defs><style>.cls-1{fill:#737373;}</style></defs>
|
||
<title>NCBI Insights Blog</title>
|
||
<path class="cls-1" d="M14,30a4,4,0,1,1-4-4,4,4,0,0,1,4,4Zm11,3A19,19,0,0,0,7.05,15a1,1,0,0,0-1,1v3a1,1,0,0,0,.93,1A14,14,0,0,1,20,33.07,1,1,0,0,0,21,34h3a1,1,0,0,0,1-1Zm9,0A28,28,0,0,0,7,6,1,1,0,0,0,6,7v3a1,1,0,0,0,1,1A23,23,0,0,1,29,33a1,1,0,0,0,1,1h3A1,1,0,0,0,34,33Z"></path>
|
||
</svg>
|
||
</a>
|
||
</div>
|
||
</div>
|
||
</section>
|
||
|
||
<section class="container-fluid bg-primary">
|
||
<div class="container pt-5">
|
||
<div class="row mt-3">
|
||
<div class="col-lg-3 col-12">
|
||
<p><a class="text-white" href="https://www.nlm.nih.gov/socialmedia/index.html">Connect with NLM</a></p>
|
||
<ul class="list-inline social_media">
|
||
<li class="list-inline-item"><a href="https://twitter.com/NLM_NIH" aria-label="Twitter" target="_blank" rel="noopener noreferrer">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
||
<title>Twitter</title>
|
||
<g id="twitterx1009" clip-path="url(#clip0_65276_3946)">
|
||
<path id="Vector_Twitter" d="M17.5006 34.6565C26.9761 34.6565 34.6575 26.9751 34.6575 17.4996C34.6575 8.02416 26.9761 0.342773 17.5006 0.342773C8.02514 0.342773 0.34375 8.02416 0.34375 17.4996C0.34375 26.9751 8.02514 34.6565 17.5006 34.6565Z" fill="#205493" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
||
<path id="path1009" d="M8.54811 8.5L16.2698 18.4279L8.50001 26.5H11.5L17.5 20L22.5486 26.5H28.5L20.5 16L27 8.5H24.5L19 14.5L14.5007 8.5H8.54927H8.54811ZM11.1197 9.73873H13.4519L25.4519 25.2613H23.1926L11.1197 9.73873Z" fill="white"></path>
|
||
</g>
|
||
<defs>
|
||
<clipPath id="clip0_65276_3946">
|
||
<rect width="35" height="35" fill="white"></rect>
|
||
</clipPath>
|
||
</defs>
|
||
</svg>
|
||
</a></li>
|
||
<li class="list-inline-item"><a href="https://www.facebook.com/nationallibraryofmedicine" aria-label="Facebook" rel="noopener noreferrer" target="_blank">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
||
<title>Facebook</title>
|
||
<g id="Facebook" clip-path="url(#clip0_1717_1086)">
|
||
<path id="Vector_Facebook" d="M15.1147 29.1371C15.1147 29.0822 15.1147 29.0296 15.1147 28.9747V18.9414H11.8183C11.6719 18.9414 11.6719 18.9414 11.6719 18.8018C11.6719 17.5642 11.6719 16.3289 11.6719 15.0937C11.6719 14.9793 11.7062 14.9518 11.816 14.9518C12.8683 14.9518 13.9206 14.9518 14.9751 14.9518H15.1215V14.8329C15.1215 13.8057 15.1215 12.774 15.1215 11.7492C15.1274 10.9262 15.3148 10.1146 15.6706 9.37241C16.1301 8.38271 16.9475 7.60378 17.9582 7.19235C18.6492 6.90525 19.3923 6.76428 20.1405 6.7783C21.0029 6.79202 21.8653 6.83091 22.7278 6.86065C22.8879 6.86065 23.048 6.89496 23.2082 6.90182C23.2974 6.90182 23.3271 6.94071 23.3271 7.02993C23.3271 7.54235 23.3271 8.05477 23.3271 8.5649C23.3271 9.16882 23.3271 9.77274 23.3271 10.3767C23.3271 10.4819 23.2974 10.5139 23.1921 10.5116C22.5379 10.5116 21.8814 10.5116 21.2271 10.5116C20.9287 10.5184 20.6316 10.5528 20.3395 10.6146C20.0822 10.6619 19.8463 10.7891 19.6653 10.9779C19.4842 11.1668 19.3672 11.4078 19.3307 11.6669C19.2857 11.893 19.2612 12.1226 19.2575 12.3531C19.2575 13.1904 19.2575 14.0299 19.2575 14.8695C19.2575 14.8946 19.2575 14.9198 19.2575 14.9564H23.0229C23.1807 14.9564 23.183 14.9564 23.1624 15.1074C23.0778 15.7662 22.9885 16.425 22.9039 17.0816C22.8322 17.6321 22.7636 18.1827 22.698 18.7332C22.6729 18.9437 22.6797 18.9437 22.4693 18.9437H19.2644V28.8992C19.2644 28.9793 19.2644 29.0593 19.2644 29.1394L15.1147 29.1371Z" fill="white"></path>
|
||
<path id="Vector_2_Facebook" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
||
</g>
|
||
<defs>
|
||
<clipPath id="clip0_1717_1086">
|
||
<rect width="35" height="35" fill="white"></rect>
|
||
</clipPath>
|
||
</defs>
|
||
</svg>
|
||
</a></li>
|
||
<li class="list-inline-item"><a href="https://www.youtube.com/user/NLMNIH" aria-label="Youtube" target="_blank" rel="noopener noreferrer">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
||
<title>Youtube</title>
|
||
<g id="YouTube" clip-path="url(#clip0_1717_1101)">
|
||
<path id="Vector_Youtube" d="M26.2571 11.4791C25.9025 11.1589 25.5709 10.9576 24.228 10.834C22.5512 10.6785 20.2797 10.6556 18.564 10.6533H16.4365C14.7208 10.6533 12.4493 10.6785 10.7725 10.834C9.43196 10.9576 9.09798 11.1589 8.7434 11.4791C7.81464 12.321 7.6202 14.6268 7.59961 16.8938C7.59961 17.3178 7.59961 17.741 7.59961 18.1635C7.62706 20.4121 7.82837 22.686 8.7434 23.521C9.09798 23.8412 9.42967 24.0425 10.7725 24.1661C12.4493 24.3216 14.7208 24.3445 16.4365 24.3468H18.564C20.2797 24.3468 22.5512 24.3216 24.228 24.1661C25.5686 24.0425 25.9025 23.8412 26.2571 23.521C27.1722 22.6929 27.3735 20.451 27.4009 18.2206C27.4009 17.7402 27.4009 17.2599 27.4009 16.7795C27.3735 14.5491 27.1699 12.3072 26.2571 11.4791ZM15.5604 20.5311V14.652L20.561 17.5001L15.5604 20.5311Z" fill="white"></path>
|
||
<path id="Vector_2_Youtube" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
||
</g>
|
||
<defs>
|
||
<clipPath id="clip0_1717_1101">
|
||
<rect width="35" height="35" fill="white"></rect>
|
||
</clipPath>
|
||
</defs>
|
||
</svg>
|
||
</a></li>
|
||
</ul>
|
||
</div>
|
||
<div class="col-lg-3 col-12">
|
||
<p class="address_footer text-white">National Library of Medicine<br />
|
||
<a href="https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/@38.9959508,-77.101021,17z/data=!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb:0x19156f88b27635b8!8m2!3d38.9959508!4d-77.0988323" class="text-white" target="_blank" rel="noopener noreferrer">8600 Rockville Pike<br />
|
||
Bethesda, MD 20894</a></p>
|
||
</div>
|
||
<div class="col-lg-3 col-12 centered-lg">
|
||
<p><a href="https://www.nlm.nih.gov/web_policies.html" class="text-white">Web Policies</a><br />
|
||
<a href="https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office" class="text-white">FOIA</a><br />
|
||
<a href="https://www.hhs.gov/vulnerability-disclosure-policy/index.html" class="text-white" id="vdp">HHS Vulnerability Disclosure</a></p>
|
||
</div>
|
||
<div class="col-lg-3 col-12 centered-lg">
|
||
<p><a class="supportLink text-white" href="https://support.nlm.nih.gov/">Help</a><br />
|
||
<a href="https://www.nlm.nih.gov/accessibility.html" class="text-white">Accessibility</a><br />
|
||
<a href="https://www.nlm.nih.gov/careers/careers.html" class="text-white">Careers</a></p>
|
||
</div>
|
||
</div>
|
||
<div class="row">
|
||
<div class="col-lg-12 centered-lg">
|
||
<nav class="bottom-links">
|
||
<ul class="mt-3">
|
||
<li>
|
||
<a class="text-white" href="//www.nlm.nih.gov/">NLM</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.nih.gov/">NIH</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.hhs.gov/">HHS</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.usa.gov/">USA.gov</a>
|
||
</li>
|
||
</ul>
|
||
</nav>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</section>
|
||
<script type="text/javascript" src="/portal/portal3rc.fcgi/rlib/js/InstrumentOmnitureBaseJS/InstrumentNCBIConfigJS/InstrumentNCBIBaseJS/InstrumentPageStarterJS.js?v=1"> </script>
|
||
<script type="text/javascript" src="/portal/portal3rc.fcgi/static/js/hfjs2.js"> </script>
|
||
</div>
|
||
</div>
|
||
<!--/.footer-->
|
||
<p class="last-updated small">Last updated: 2020-12-02T23:21:12Z</p>
|
||
</div>
|
||
<!--/.page-->
|
||
</div>
|
||
<!--/.wrap-->
|
||
<span class="PAFAppResources"></span>
|
||
|
||
|
||
</div><!-- /.twelve_col -->
|
||
</div>
|
||
<!-- /.grid -->
|
||
|
||
|
||
|
||
<!-- usually for JS scripts at page bottom -->
|
||
<span class="pagefixtures"></span>
|
||
|
||
|
||
<!-- CE8B5AF87C7FFCB1_0191SID /projects/GTR/gtr1/gtr1@1.224 portal105 v4.1.r689238 Tue, Oct 22 2024 16:10:51 -->
|
||
<span id="portal-csrf-token" style="display:none" data-token="CE8B5AF87C7FFCB1_0191SID"></span>
|
||
|
||
<script type="text/javascript" src="//static.pubmed.gov/portal/portal3rc.fcgi/4221774/js/3879255/4121861/4052736/4171016/3898962/3538227/4209230.js" snapshot="gtr"></script></body>
|
||
</html>
|
||
|