611 lines
39 KiB
XML
611 lines
39 KiB
XML
<?xml version="1.0" encoding="utf-8"?>
|
|
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
|
|
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
|
|
|
|
<head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
|
|
<!-- AppResources meta begin -->
|
|
<meta name="paf-app-resources" content="" />
|
|
<!-- AppResources meta end -->
|
|
|
|
<!-- TemplateResources meta begin -->
|
|
<meta name="paf_template" content="StdNCol" />
|
|
|
|
<!-- TemplateResources meta end -->
|
|
|
|
<!-- Page meta begin -->
|
|
|
|
<!-- Page meta end -->
|
|
|
|
<!-- Logger begin -->
|
|
<meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_app" content="genbank" /><meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_pdid" content="custom-page" />
|
|
<!-- Logger end -->
|
|
|
|
<title>Targeted Locus Study (TLS) Submission Guide</title>
|
|
|
|
<!-- PageFixtures headcontent begin -->
|
|
|
|
<meta name="cms-local-nav-url" content="https://cms.ncbi.nlm.nih.gov//genbank/_nav" />
|
|
|
|
<!-- PageFixtures headcontent end -->
|
|
|
|
<!-- AppResources external_resources begin -->
|
|
<script type="text/javascript" src="/core/jig/1.15.6/js/jig.min.js"></script>
|
|
|
|
<!-- AppResources external_resources end -->
|
|
|
|
<!-- Page headcontent begin -->
|
|
<meta name="subsite" content="genbank" />
|
|
<meta name="path" content="genbank/tlsguide" />
|
|
<meta name="modified" content="2024-06-12T20:07:17Z" /><meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="cms-edit-aux-url" content="http://cms.ncbi.nlm.nih.gov/node//edit" />
|
|
<!-- Page headcontent end -->
|
|
<!-- PageFixtures resources begin -->
|
|
<link xmlns="http://www.w3.org/1999/xhtml" type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218191/css/4207974/4206132.css" xml:base="http://127.0.0.1/sites/static/header_footer" />
|
|
|
|
<!-- PageFixtures resources end -->
|
|
<link rel="shortcut icon" href="//www.ncbi.nlm.nih.gov/favicon.ico" /><meta name="ncbi_phid" content="CE8CA8637C800B210000000000080008.m_7" />
|
|
<meta name='referrer' content='origin-when-cross-origin'/><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/css/4121862/3974050/3917732/251717/4108189/14534/45193/3534283/4128070/3407145/4005757/4062871.css" /><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/css/3529741/3529739.css" media="print" /></head>
|
|
<body class=" col2 custom-page">
|
|
<div class="grid">
|
|
<div class="col twelve_col nomargin shadow">
|
|
<!-- System messages like service outage or JS required; this is handled by the TemplateResources portlet -->
|
|
<div class="sysmessages">
|
|
<noscript>
|
|
<p class="nojs">
|
|
<strong>Warning:</strong>
|
|
The NCBI web site requires JavaScript to function.
|
|
<a href="/guide/browsers/#enablejs" title="Learn how to enable JavaScript" target="_blank">more...</a>
|
|
</p>
|
|
</noscript>
|
|
</div>
|
|
<!--/.sysmessage-->
|
|
<div class="wrap">
|
|
<div class="page">
|
|
<div xmlns:xi="http://www.w3.org/2001/XInclude">
|
|
<div xmlns="http://www.w3.org/1999/xhtml" id="universal_header" xml:base="http://127.0.0.1/sites/static/header_footer">
|
|
<section class="usa-banner">
|
|
<div class="usa-accordion">
|
|
<header class="usa-banner-header">
|
|
<div class="usa-grid usa-banner-inner">
|
|
<img src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/favicons/favicon-57.png" alt="U.S. flag" />
|
|
<p>An official website of the United States government</p>
|
|
<button class="non-usa-accordion-button usa-banner-button" aria-expanded="false" aria-controls="gov-banner-top" type="button">
|
|
<span class="usa-banner-button-text">Here's how you know</span>
|
|
</button>
|
|
</div>
|
|
</header>
|
|
<div class="usa-banner-content usa-grid usa-accordion-content" id="gov-banner-top" aria-hidden="true">
|
|
<div class="usa-banner-guidance-gov usa-width-one-half">
|
|
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-dot-gov.svg" alt="Dot gov" />
|
|
<div class="usa-media_block-body">
|
|
<p>
|
|
<strong>The .gov means it's official.</strong>
|
|
<br />
|
|
Federal government websites often end in .gov or .mil. Before
|
|
sharing sensitive information, make sure you're on a federal
|
|
government site.
|
|
</p>
|
|
</div>
|
|
</div>
|
|
<div class="usa-banner-guidance-ssl usa-width-one-half">
|
|
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-https.svg" alt="Https" />
|
|
<div class="usa-media_block-body">
|
|
<p>
|
|
<strong>The site is secure.</strong>
|
|
<br />
|
|
The <strong>https://</strong> ensures that you are connecting to the
|
|
official website and that any information you provide is encrypted
|
|
and transmitted securely.
|
|
</p>
|
|
</div>
|
|
</div>
|
|
</div>
|
|
</div>
|
|
</section>
|
|
<div class="usa-overlay"></div>
|
|
<header class="ncbi-header" role="banner" data-section="Header">
|
|
|
|
<div class="usa-grid">
|
|
<div class="usa-width-one-whole">
|
|
|
|
<div class="ncbi-header__logo">
|
|
<a href="/" class="logo" aria-label="NCBI Logo" data-ga-action="click_image" data-ga-label="NIH NLM Logo">
|
|
<img src="https://www.ncbi.nlm.nih.gov/coreutils/nwds/img/logos/AgencyLogo.svg" alt="NIH NLM Logo" />
|
|
</a>
|
|
</div>
|
|
|
|
<div class="ncbi-header__account">
|
|
<a id="account_login" href="https://account.ncbi.nlm.nih.gov" class="usa-button header-button" style="display:none" data-ga-action="open_menu" data-ga-label="account_menu">Log in</a>
|
|
<button id="account_info" class="header-button" style="display:none" aria-controls="account_popup" type="button">
|
|
<span class="fa fa-user" aria-hidden="true">
|
|
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24" width="20px" height="20px">
|
|
<g style="fill: #fff">
|
|
<ellipse cx="12" cy="8" rx="5" ry="6"></ellipse>
|
|
<path d="M21.8,19.1c-0.9-1.8-2.6-3.3-4.8-4.2c-0.6-0.2-1.3-0.2-1.8,0.1c-1,0.6-2,0.9-3.2,0.9s-2.2-0.3-3.2-0.9 C8.3,14.8,7.6,14.7,7,15c-2.2,0.9-3.9,2.4-4.8,4.2C1.5,20.5,2.6,22,4.1,22h15.8C21.4,22,22.5,20.5,21.8,19.1z"></path>
|
|
</g>
|
|
</svg>
|
|
</span>
|
|
<span class="username desktop-only" aria-hidden="true" id="uname_short"></span>
|
|
<span class="sr-only">Show account info</span>
|
|
</button>
|
|
</div>
|
|
|
|
<div class="ncbi-popup-anchor">
|
|
<div class="ncbi-popup account-popup" id="account_popup" aria-hidden="true">
|
|
<div class="ncbi-popup-head">
|
|
<button class="ncbi-close-button" data-ga-action="close_menu" data-ga-label="account_menu" type="button">
|
|
<span class="fa fa-times">
|
|
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 48 48" width="24px" height="24px">
|
|
<path d="M38 12.83l-2.83-2.83-11.17 11.17-11.17-11.17-2.83 2.83 11.17 11.17-11.17 11.17 2.83 2.83 11.17-11.17 11.17 11.17 2.83-2.83-11.17-11.17z"></path>
|
|
</svg>
|
|
</span>
|
|
<span class="usa-sr-only">Close</span></button>
|
|
<h4>Account</h4>
|
|
</div>
|
|
<div class="account-user-info">
|
|
Logged in as:<br />
|
|
<b><span class="username" id="uname_long">username</span></b>
|
|
</div>
|
|
<div class="account-links">
|
|
<ul class="usa-unstyled-list">
|
|
<li><a id="account_myncbi" href="/myncbi/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_myncbi">Dashboard</a></li>
|
|
<li><a id="account_pubs" href="/myncbi/collections/bibliography/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_pubs">Publications</a></li>
|
|
<li><a id="account_settings" href="/account/settings/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_settings">Account settings</a></li>
|
|
<li><a id="account_logout" href="/account/signout/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_logout">Log out</a></li>
|
|
</ul>
|
|
</div>
|
|
</div>
|
|
</div>
|
|
|
|
</div>
|
|
</div>
|
|
</header>
|
|
<div role="navigation" aria-label="access keys">
|
|
<a id="nws_header_accesskey_0" href="https://www.ncbi.nlm.nih.gov/guide/browsers/#ncbi_accesskeys" class="usa-sr-only" accesskey="0" tabindex="-1">Access keys</a>
|
|
<a id="nws_header_accesskey_1" href="https://www.ncbi.nlm.nih.gov" class="usa-sr-only" accesskey="1" tabindex="-1">NCBI Homepage</a>
|
|
<a id="nws_header_accesskey_2" href="/myncbi/" class="set-base-url usa-sr-only" accesskey="2" tabindex="-1">MyNCBI Homepage</a>
|
|
<a id="nws_header_accesskey_3" href="#maincontent" class="usa-sr-only" accesskey="3" tabindex="-1">Main Content</a>
|
|
<a id="nws_header_accesskey_4" href="#" class="usa-sr-only" accesskey="4" tabindex="-1">Main Navigation</a>
|
|
</div>
|
|
<section data-section="Alerts">
|
|
<div class="ncbi-alerts-placeholder"></div>
|
|
</section>
|
|
</div>
|
|
</div>
|
|
<!--/.header-->
|
|
<div class="header">
|
|
<div class="res_logo"><h1 class="res_name"><a href="/genbank/" title="GenBank home">GenBank</a></h1><h2 class="res_tagline">Public nucleic acid sequence repository</h2></div>
|
|
<div class="search"><form method="get" action="/nuccore/"><div class="search_form"><label for="database" class="offscreen_noflow">Search database</label><select id="database"><optgroup label="Recent"><option value="nuccore" selected="selected">Nucleotide</option><option value="sra">SRA</option><option value="books">Books</option><option value="clinvar" class="last">ClinVar</option></optgroup><optgroup label="All"><option value="gquery">All Databases</option><option value="assembly">Assembly</option><option value="biocollections">Biocollections</option><option value="bioproject">BioProject</option><option value="biosample">BioSample</option><option value="books">Books</option><option value="clinvar">ClinVar</option><option value="cdd">Conserved Domains</option><option value="gap">dbGaP</option><option value="dbvar">dbVar</option><option value="gene">Gene</option><option value="genome">Genome</option><option value="gds">GEO DataSets</option><option value="geoprofiles">GEO Profiles</option><option value="gtr">GTR</option><option value="ipg">Identical Protein Groups</option><option value="medgen">MedGen</option><option value="mesh">MeSH</option><option value="nlmcatalog">NLM Catalog</option><option value="nuccore">Nucleotide</option><option value="omim">OMIM</option><option value="pmc">PMC</option><option value="protein">Protein</option><option value="proteinclusters">Protein Clusters</option><option value="protfam">Protein Family Models</option><option value="pcassay">PubChem BioAssay</option><option value="pccompound">PubChem Compound</option><option value="pcsubstance">PubChem Substance</option><option value="pubmed">PubMed</option><option value="snp">SNP</option><option value="sra">SRA</option><option value="structure">Structure</option><option value="taxonomy">Taxonomy</option><option value="toolkit">ToolKit</option><option value="toolkitall">ToolKitAll</option><option value="toolkitbookgh">ToolKitBookgh</option></optgroup></select><div class="nowrap"><label for="term" class="offscreen_noflow" accesskey="/">Search term</label><div class="nowrap"><input type="text" name="term" id="term" title="Search Nucleotide" value="" class="jig-ncbiclearbutton jig-ncbiautocomplete" data-jigconfig="isEnabled:false,disableUrl:'NcbiSearchBarAutoComplCtrl'" autocomplete="off" data-sbconfig="ds:'no',pjs:'no',afs:'yes'" /></div><button id="search" type="submit" class="button_search nowrap" cmd="go">Search</button></div></div></form></div>
|
|
|
|
</div>
|
|
<div class="nav_and_browser">
|
|
<div class="localnav"><ul class="jig-ncbilocalnav">
|
|
<li><a href="#">GenBank</a><ul>
|
|
<li><a href="/genbank/">About GenBank</a></li>
|
|
<li><a href="/genbank/submit_types">Submission Types</a></li>
|
|
<li><a href="/genbank/submit">Submission Tools</a></li>
|
|
<li><a href="/genbank/update">Update GenBank Records</a></li>
|
|
<li><a href="/nuccore/">Search</a></li>
|
|
<li><a href="/BLAST/Blast.cgi?CMD=Web&PAGETYPE=BLASTHome">BLAST</a></li>
|
|
<li><a href="/genbank/statistics">Statistics</a></li>
|
|
<li><a href="/genbank/samplerecord/">Sample Record</a></li>
|
|
<li><a href="/genbank/sequencerevisionhistory/">Revision History</a></li>
|
|
<li><a href="/genbank/sequenceids/">Sequence IDs</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">Submit</a><ul>
|
|
<li><a href="/genbank/submit">Submission Tools</a></li>
|
|
<li><a href="/genbank/submit_types">Submission Types</a></li>
|
|
<li><a href="/WebSub/?tool=genbank">BankIt</a></li>
|
|
<li><a href="/genbank/table2asn">table2asn</a></li>
|
|
<li><a href="https://www.ncbi.nlm.nih.gov/sra/docs/sequence-data-processing">Sequence Data Processing</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">Genomes</a><ul>
|
|
<li><a href="/genbank/genomesubmit">Complete Genome Submission Guide</a></li>
|
|
<li><a href="/genbank/genomesubmit_annotation">Prokaryotic Genome Annotation Guide</a></li>
|
|
<li><a href="/genbank/eukaryotic_genome_submission_annotation">Eukaryotic Genome Annotation Guide</a></li>
|
|
<li><a href="/genbank/examples.wgs">Annotation Examples</a></li>
|
|
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/wgs/">Genome Submission Portal</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a title="Whole Genome Shotgun sequences and submissions" href="#">WGS</a><ul>
|
|
<li><a href="/genbank/wgs">About WGS</a></li>
|
|
<li><a href="/Traces/wgs">WGS Project List</a></li>
|
|
<li><a href="/genbank/wgs.submit">WGS Submission Guide</a></li>
|
|
<li><a href="/genbank/wgsfaq/">FAQ</a></li>
|
|
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/wgs/">Genome Submission Portal</a></li>
|
|
<li><a href="/genbank/eukaryotic_genome_submission_annotation">Eukaryotic Annotation Guide</a></li>
|
|
<li><a href="/genbank/genomesubmit_annotation">Prokaryotic Annotation Guide</a></li>
|
|
<li><a href="/genbank/asndisc">Discrepancy Report</a></li>
|
|
<li><a href="/assembly/agp/AGP_Specification/">AGP format</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">Metagenomes</a><ul>
|
|
<li><a href="/genbank/metagenome">About Metagenomes</a></li>
|
|
<li><a href="/genbank/structuredcomment">Structured Comment</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">TPA</a><ul>
|
|
<li><a href="/genbank/TPA">About TPA</a></li>
|
|
<li><a href="/genbank/tpafaq">FAQ</a></li>
|
|
<li><a href="/genbank/TPA-Exp">TPA-Exp</a></li>
|
|
<li><a href="/genbank/TPA-Inf">TPA-Inf</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">TSA</a><ul>
|
|
<li><a href="/genbank/TSA">About TSA</a></li>
|
|
<li><a href="/genbank/TSAguide">TSA Submission Guide</a></li>
|
|
<li><a href="/genbank/TSAfaq">FAQ</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">INSDC</a><ul>
|
|
<li><a href="/genbank/collab">About INSDC</a></li>
|
|
<li><a href="/genbank/collab/country">Geographic Location Name List</a></li>
|
|
<li><a href="/genbank/collab/db_xref">db_xref List</a></li>
|
|
<li><a href="http://www.insdc.org/documents/feature_table.html">Feature Table</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">Documentation</a><ul>
|
|
<li><a href="https://www.ncbi.nlm.nih.gov/sra/docs/sequence-data-processing/">Sequence Data Processing</a></li>
|
|
<li><a href="/genbank/submission_brokers">Submission Brokers</a></li>
|
|
<li><a href="/genbank/acc_prefix">Accession Number Prefixes</a></li>
|
|
<li><a href="/genbank/organelle_submit/">Organelle Submission Guide</a></li>
|
|
<li><a href="/genbank/monkeypox_submission/">Monkeypox Submission Guide</a></li>
|
|
<li><a href="/genbank/validation/">Common Submission Errors</a> </li>
|
|
<li><a href="/genbank/sequencecheck/">Ribosomal Submission Errors</a></li>
|
|
<li><a href="/genbank/sequencecheck/virus">Common Sequence Errors</a></li>
|
|
<li><a href="https://support.nlm.nih.gov/knowledgebase/category/?id=CAT-01240">Submission FAQs</a></li>
|
|
</ul>
|
|
</li>
|
|
<li><a href="#">Other</a><ul>
|
|
<li><a href="/genbank/htgs">About HTGs</a></li>
|
|
<li><a href="/genbank/dbest">About EST</a></li>
|
|
<li><a href="/genbank/dbgss">About GSS</a></li>
|
|
<li><a href="/genbank/tls">About TLS</a></li>
|
|
<li><a href="/genbank/tlsguide">Submit TLS</a></li>
|
|
</ul>
|
|
</li>
|
|
</ul></div>
|
|
</div>
|
|
|
|
<!-- was itemctrl -->
|
|
<div class="container">
|
|
<div id="maincontent" class="content col twelve_col last">
|
|
<div class="col1">
|
|
<h1 id="targeted-locus-study-tls-submiss">Targeted Locus Study (TLS) Submission Guide</h1>
|
|
|
|
|
|
<h2 id="prerequisites">Prerequisites</h2>
|
|
|
|
|
|
<ul>
|
|
<li>Submission of sequence reads to <a href="https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi">SRA</a> is highly recommended.</li>
|
|
<li>
|
|
<p><a href="https://www.ncbi.nlm.nih.gov/bioproject/">BioProject</a> and <a href="https://www.ncbi.nlm.nih.gov/biosample/">BioSample</a> IDs. </p>
|
|
<ul>
|
|
<li>The <a href="https://submit.ncbi.nlm.nih.gov/subs/genbank/">NCBI Submission Portal</a> includes steps for BioProject and BioSample registration during the submission of large scale prokaryotic 16S ribosomal rRNA TLS projects. Previously assigned BioProject and BioSample IDs can also be provided within the submission wizard.</li>
|
|
<li>Study and sample metadata must be submitted to BioProject and BioSample prior to submission of other types of TLS projects. If sequence reads were already submitted, provide the assigned BioProject and BioSample IDs during submission.</li>
|
|
</ul>
|
|
</li>
|
|
</ul>
|
|
|
|
|
|
<h2 id="creating-the-tls-submission-file">Creating the TLS submission file</h2>
|
|
|
|
|
|
<p>[1] Sequences</p>
|
|
|
|
|
|
<ul>
|
|
<li>Each individual fasta file should include sequences derived from a single large scale study. The total number of sequences included within all fasta files that are part of a single project should be >2,500.</li>
|
|
<li>A unique ID should be included for each sequence within the fasta file. These IDs will be included within the Definition line on the GenBank flatfile for each <a href="https://www.ncbi.nlm.nih.gov/Traces/wgs/KBUV01/KBUV01000001.1">individual sequence</a>. This sequence ID can represent the OTU, phylotype, or other unique sequence identifier. </li>
|
|
<li>Remove vector, chimeras, low quality sequence and questionable data from your sequences before submitting.</li>
|
|
<li>More information about the format for the fasta file and sequence requirements can be found <a href="https://submit.ncbi.nlm.nih.gov/genbank/help/#fasta">here</a>.</li>
|
|
</ul>
|
|
|
|
|
|
<p>[2] Project Information</p>
|
|
|
|
|
|
<p>Include a description of the large scale/TLS study within a <a href="https://www.ncbi.nlm.nih.gov/bioproject/">BioProject</a>. The BioProject ID will be included on the TLS master flatfile and functions to provide a single link to all the data types that are part of the project. The same BioProject ID should be included with all submissions that are part of the same study.</p>
|
|
|
|
|
|
<p>[3] Source Information</p>
|
|
|
|
|
|
<p>Source metadata should be included within <a href="https://www.ncbi.nlm.nih.gov/biosample/">BioSample</a> using the appropriate package. Details regarding the requirements for each BioSample package can be found <a href="https://submit.ncbi.nlm.nih.gov/biosample/template/">here</a>. The GenBank submission wizard for prokaryotic 16S rRNA sequences within the <a href="https://submit.ncbi.nlm.nih.gov/subs/genbank/">NCBI Submission Portal</a> allows the creation of BioSamples using these package types:</p>
|
|
|
|
|
|
<ul>
|
|
<li>Pathogen affecting public health</li>
|
|
<li>Metagenome or environmental sample</li>
|
|
<li>Genome, metagenome or marker sequences (MIxS compliant): MIMS and MIMARKS (survey)</li>
|
|
</ul>
|
|
|
|
|
|
<p>All submissions should include rich contextual information about where the specimen was obtained, including but not limited to: isolation-source or host, collection date, geographic location name, and latitude/longitude. Uncultured samples require a metagenome organism name (eg., marine metagenome) that will be applied to the entire TLS submission. If more descriptive organism names are needed, please send a request to gb-admin@ncbi.nlm.nih.gov prior to submitting your files.</p>
|
|
|
|
|
|
<p>If the sequences in the submission were obtained from multiple BioSamples, a tab-delimited mapping file is required that lists the BioSample that should be included for each sequence in your submission. This mapping file should include the BioSample Accessions that were assigned if the samples were registered prior to sequence submission. If BioSamples are created within the 16S ribosomal RNA Submission Wizard, the sample names should be used. Currently, only a single BioSample ID can be included per sequence. If multiple BioSample IDs should be included per sequence, please contact <a href="mailto:gb-admin@ncbi.nlm.nih.gov">gb-admin@ncbi.nlm.nih.gov</a>.</p>
|
|
|
|
|
|
<p>[4] Features</p>
|
|
|
|
|
|
<ul>
|
|
<li>The ribosomal RNA submission wizard will incorporate the appropriate feature annotation.</li>
|
|
<li>Other sequence types, such as a single locus or conserved element should include the appropriate feature type (eg., gene, misc_feature). More information regarding the options for submitting data to GenBank can be found <a href="https://www.ncbi.nlm.nih.gov/genbank/submit/">here</a>.</li>
|
|
</ul>
|
|
|
|
|
|
<h2 id="submitting-tls-files">Submitting TLS Files</h2>
|
|
|
|
|
|
<ul>
|
|
<li>The <a href="https://submit.ncbi.nlm.nih.gov/subs/genbank/">NCBI Submission Portal</a> GenBank wizard should be used to submit ribosomal RNA sequences.</li>
|
|
<li>Other targeted locus study submissions should be submitted to GenBank using <a href="https://www.ncbi.nlm.nih.gov/genbank/table2asn/">table2asn</a> to create submission files that can be emailed to gb-sub@ncbi.nlm.nih.gov.</li>
|
|
</ul>
|
|
|
|
|
|
<h2 id="tls-16s-rrna-sequence-analysis">TLS 16S rRNA Sequence Analysis</h2>
|
|
|
|
|
|
<p>Ribosomal RNA sequences are checked for a number of issues before they are accepted for GenBank. A summary of these checks can be found <a href="https://www.ncbi.nlm.nih.gov/genbank/sequencecheck/">here</a>. These include chimera analysis, vector screens, and sequence length.</p>
|
|
|
|
|
|
<p>Large scale submissions of 16S rRNA from uncultured prokaryotes that are processed as TLS projects are verified using an additional analysis program <a href="https://github.com/nawrockie/ribosensor_wrapper">Ribosensor</a> (version 0.27). In this analysis, each sequence is BLASTed against a rRNA reference dataset as well as compared to sets of profile HMMs built from representative alignments of SSU rRNA sequences. Each profile HMM model is built from a multiple alignment of 50-100 representative sequences from the family. The source of several of the alignments, including the bacterial model, is the Rfam database (rfam.xfam.org). Each sequence is aligned to each profile and a score is computed based on how well the sequence matches the profile. Each sequence is classified by the model that gives it the highest score. The BLAST and profile HMM results are combined and sequences with unexpected features are reported. Some examples of unexpected features that are detected are low scores, low coverage, and duplicated regions suggestive of misassembly.</p>
|
|
|
|
|
|
<p>You will be notified during submission processing if your sequences have any of these issues. If you have questions, please write to: gb-admin@ncbi.nlm.nih.gov and include your submission number.</p>
|
|
|
|
|
|
<h2 id="updating-tls-submissions">Updating TLS Submissions</h2>
|
|
|
|
|
|
<ul>
|
|
<li>If you are updating a publication, send the TLS accession prefix and complete publication information in the text portion of an email to gb-admin@ncbi.nlm.nih.gov.</li>
|
|
<li>
|
|
<p>If you are updating any other information, do not create a new submission. Please contact gb-admin@ncbi.nlm.nih.gov for directions and include the following information with your request:</p>
|
|
<ul>
|
|
<li>Description of your update</li>
|
|
<li>TLS Accession prefix</li>
|
|
</ul>
|
|
<p>We will send instructions on how to proceed with the requested update.</p>
|
|
</li>
|
|
</ul>
|
|
|
|
|
|
<pre><code>
|
|
</code></pre>
|
|
</div>
|
|
<!--/.col1-->
|
|
<div class="col2">
|
|
<div class="rightnav">
|
|
<h2 id="tls-resources">TLS Resources</h2>
|
|
<ul>
|
|
<li><a href="/genbank/tls">About TLS</a></li>
|
|
<li><a href="/genbank/tlsguide">TLS Submission Guide</a> </li>
|
|
<li><a href="/genbank/table2asn">table2asn</a></li>
|
|
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/">NCBI Submission Portal</a></li>
|
|
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/bioproject/">BioProject</a></li>
|
|
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/biosample/">BioSample</a></li>
|
|
<li><a href="https://www.ncbi.nlm.nih.gov/Traces/wgs/?view=TLS">Sequence Set Browser</a></li>
|
|
<li><a href="https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi">SRA</a></li>
|
|
</ul>
|
|
</div>
|
|
</div>
|
|
<!--/.col2-->
|
|
<div class="col3">
|
|
|
|
</div>
|
|
<!--/.col3-->
|
|
<div class="col4">
|
|
|
|
</div>
|
|
<!--/.col4-->
|
|
<div class="col5">
|
|
|
|
</div>
|
|
<div class="col6">
|
|
|
|
</div>
|
|
<div class="col7">
|
|
|
|
</div>
|
|
<div class="col8">
|
|
|
|
</div>
|
|
<div class="col9">
|
|
|
|
</div>
|
|
</div><!--/.content-->
|
|
</div><!--/.container-->
|
|
<div id="NCBIFooter_dynamic">
|
|
<div class="breadcrumbs">You are here:
|
|
<span id="breadcrumb_text"><a href="/guide/">NCBI</a></span></div>
|
|
<a id="help-desk-link" class="help_desk" href="https://support.ncbi.nlm.nih.gov/ics/support/default.asp?Time=2025-03-05T02:45:55-05:00&Snapshot=%2Fprojects%2Fstaticsites%2Fgenbank%2Fgenbank@2.21&Host=portal105&ncbi_phid=CE8CA8637C800B210000000000080008&ncbi_session=CE8B5AF87C7FFCB1_0191SID&from=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fgenbank%2Ftlsguide%2F&Ncbi_App=genbank&Page=custom-page&style=classic&deptID=28049" target="_blank">Support Center</a>
|
|
<noscript><img alt="" src="/stat?jsdisabled=true&ncbi_app=genbank&ncbi_db=&ncbi_pdid=custom-page&ncbi_phid=CE8CA8637C800B210000000000080008" /></noscript>
|
|
</div>
|
|
|
|
|
|
<div xmlns:xi="http://www.w3.org/2001/XInclude">
|
|
<div xmlns="http://www.w3.org/1999/xhtml" class="footer" id="footer" xml:base="http://127.0.0.1/sites/static/header_footer">
|
|
<section class="icon-section">
|
|
<div id="icon-section-header" class="icon-section_header">Follow NCBI</div>
|
|
<div class="grid-container container">
|
|
<div class="icon-section_container">
|
|
<a class="footer-icon" id="footer_twitter" href="https://twitter.com/ncbi" aria-label="Twitter">
|
|
<svg xmlns="http://www.w3.org/2000/svg" width="40" height="40" viewBox="0 0 40 40" fill="none">
|
|
<title>Twitter</title>
|
|
<g id="twitterx1008">
|
|
<path id="path1008" d="M6.06736 7L16.8778 20.8991L6.00001 32.2H10.2L18.6 23.1L25.668 32.2H34L22.8 17.5L31.9 7H28.4L20.7 15.4L14.401 7H6.06898H6.06736ZM9.66753 8.73423H12.9327L29.7327 30.4658H26.5697L9.66753 8.73423Z" fill="#5B616B"></path>
|
|
</g>
|
|
</svg>
|
|
</a>
|
|
<a class="footer-icon" id="footer_facebook" href="https://www.facebook.com/ncbi.nlm" aria-label="Facebook"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
|
<title>Facebook</title>
|
|
<path class="cls-11" d="M210.5,115.12H171.74V97.82c0-8.14,5.39-10,9.19-10h27.14V52l-39.32-.12c-35.66,0-42.42,26.68-42.42,43.77v19.48H99.09v36.32h27.24v109h45.41v-109h35Z">
|
|
</path>
|
|
</svg></a>
|
|
<a class="footer-icon" id="footer_linkedin" href="https://www.linkedin.com/company/ncbinlm" aria-label="LinkedIn"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
|
<title>LinkedIn</title>
|
|
<path class="cls-11" d="M101.64,243.37H57.79v-114h43.85Zm-22-131.54h-.26c-13.25,0-21.82-10.36-21.82-21.76,0-11.65,8.84-21.15,22.33-21.15S101.7,78.72,102,90.38C102,101.77,93.4,111.83,79.63,111.83Zm100.93,52.61A17.54,17.54,0,0,0,163,182v61.39H119.18s.51-105.23,0-114H163v13a54.33,54.33,0,0,1,34.54-12.66c26,0,44.39,18.8,44.39,55.29v58.35H198.1V182A17.54,17.54,0,0,0,180.56,164.44Z">
|
|
</path>
|
|
</svg></a>
|
|
<a class="footer-icon" id="footer_github" href="https://github.com/ncbi" aria-label="GitHub"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
|
<defs>
|
|
<style>
|
|
.cls-11,
|
|
.cls-12 {
|
|
fill: #737373;
|
|
}
|
|
|
|
.cls-11 {
|
|
fill-rule: evenodd;
|
|
}
|
|
</style>
|
|
</defs>
|
|
<title>GitHub</title>
|
|
<path class="cls-11" d="M151.36,47.28a105.76,105.76,0,0,0-33.43,206.1c5.28,1,7.22-2.3,7.22-5.09,0-2.52-.09-10.85-.14-19.69-29.42,6.4-35.63-12.48-35.63-12.48-4.81-12.22-11.74-15.47-11.74-15.47-9.59-6.56.73-6.43.73-6.43,10.61.75,16.21,10.9,16.21,10.9,9.43,16.17,24.73,11.49,30.77,8.79,1-6.83,3.69-11.5,6.71-14.14C108.57,197.1,83.88,188,83.88,147.51a40.92,40.92,0,0,1,10.9-28.39c-1.1-2.66-4.72-13.42,1-28,0,0,8.88-2.84,29.09,10.84a100.26,100.26,0,0,1,53,0C198,88.3,206.9,91.14,206.9,91.14c5.76,14.56,2.14,25.32,1,28a40.87,40.87,0,0,1,10.89,28.39c0,40.62-24.74,49.56-48.29,52.18,3.79,3.28,7.17,9.71,7.17,19.58,0,14.15-.12,25.54-.12,29,0,2.82,1.9,6.11,7.26,5.07A105.76,105.76,0,0,0,151.36,47.28Z">
|
|
</path>
|
|
<path class="cls-12" d="M85.66,199.12c-.23.52-1.06.68-1.81.32s-1.2-1.06-.95-1.59,1.06-.69,1.82-.33,1.21,1.07.94,1.6Zm-1.3-1">
|
|
</path>
|
|
<path class="cls-12" d="M90,203.89c-.51.47-1.49.25-2.16-.49a1.61,1.61,0,0,1-.31-2.19c.52-.47,1.47-.25,2.17.49s.82,1.72.3,2.19Zm-1-1.08">
|
|
</path>
|
|
<path class="cls-12" d="M94.12,210c-.65.46-1.71,0-2.37-.91s-.64-2.07,0-2.52,1.7,0,2.36.89.65,2.08,0,2.54Zm0,0"></path>
|
|
<path class="cls-12" d="M99.83,215.87c-.58.64-1.82.47-2.72-.41s-1.18-2.06-.6-2.7,1.83-.46,2.74.41,1.2,2.07.58,2.7Zm0,0">
|
|
</path>
|
|
<path class="cls-12" d="M107.71,219.29c-.26.82-1.45,1.2-2.64.85s-2-1.34-1.74-2.17,1.44-1.23,2.65-.85,2,1.32,1.73,2.17Zm0,0">
|
|
</path>
|
|
<path class="cls-12" d="M116.36,219.92c0,.87-1,1.59-2.24,1.61s-2.29-.68-2.3-1.54,1-1.59,2.26-1.61,2.28.67,2.28,1.54Zm0,0">
|
|
</path>
|
|
<path class="cls-12" d="M124.42,218.55c.15.85-.73,1.72-2,1.95s-2.37-.3-2.52-1.14.73-1.75,2-2,2.37.29,2.53,1.16Zm0,0"></path>
|
|
</svg></a>
|
|
<a class="footer-icon" id="footer_blog" href="https://ncbiinsights.ncbi.nlm.nih.gov/" aria-label="Blog">
|
|
<svg xmlns="http://www.w3.org/2000/svg" id="Layer_1" data-name="Layer 1" viewBox="0 0 40 40">
|
|
<defs><style>.cls-1{fill:#737373;}</style></defs>
|
|
<title>NCBI Insights Blog</title>
|
|
<path class="cls-1" d="M14,30a4,4,0,1,1-4-4,4,4,0,0,1,4,4Zm11,3A19,19,0,0,0,7.05,15a1,1,0,0,0-1,1v3a1,1,0,0,0,.93,1A14,14,0,0,1,20,33.07,1,1,0,0,0,21,34h3a1,1,0,0,0,1-1Zm9,0A28,28,0,0,0,7,6,1,1,0,0,0,6,7v3a1,1,0,0,0,1,1A23,23,0,0,1,29,33a1,1,0,0,0,1,1h3A1,1,0,0,0,34,33Z"></path>
|
|
</svg>
|
|
</a>
|
|
</div>
|
|
</div>
|
|
</section>
|
|
|
|
<section class="container-fluid bg-primary">
|
|
<div class="container pt-5">
|
|
<div class="row mt-3">
|
|
<div class="col-lg-3 col-12">
|
|
<p><a class="text-white" href="https://www.nlm.nih.gov/socialmedia/index.html">Connect with NLM</a></p>
|
|
<ul class="list-inline social_media">
|
|
<li class="list-inline-item"><a href="https://twitter.com/NLM_NIH" aria-label="Twitter" target="_blank" rel="noopener noreferrer">
|
|
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
|
<title>Twitter</title>
|
|
<g id="twitterx1009" clip-path="url(#clip0_65276_3946)">
|
|
<path id="Vector_Twitter" d="M17.5006 34.6565C26.9761 34.6565 34.6575 26.9751 34.6575 17.4996C34.6575 8.02416 26.9761 0.342773 17.5006 0.342773C8.02514 0.342773 0.34375 8.02416 0.34375 17.4996C0.34375 26.9751 8.02514 34.6565 17.5006 34.6565Z" fill="#205493" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
|
<path id="path1009" d="M8.54811 8.5L16.2698 18.4279L8.50001 26.5H11.5L17.5 20L22.5486 26.5H28.5L20.5 16L27 8.5H24.5L19 14.5L14.5007 8.5H8.54927H8.54811ZM11.1197 9.73873H13.4519L25.4519 25.2613H23.1926L11.1197 9.73873Z" fill="white"></path>
|
|
</g>
|
|
<defs>
|
|
<clipPath id="clip0_65276_3946">
|
|
<rect width="35" height="35" fill="white"></rect>
|
|
</clipPath>
|
|
</defs>
|
|
</svg>
|
|
</a></li>
|
|
<li class="list-inline-item"><a href="https://www.facebook.com/nationallibraryofmedicine" aria-label="Facebook" rel="noopener noreferrer" target="_blank">
|
|
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
|
<title>Facebook</title>
|
|
<g id="Facebook" clip-path="url(#clip0_1717_1086)">
|
|
<path id="Vector_Facebook" d="M15.1147 29.1371C15.1147 29.0822 15.1147 29.0296 15.1147 28.9747V18.9414H11.8183C11.6719 18.9414 11.6719 18.9414 11.6719 18.8018C11.6719 17.5642 11.6719 16.3289 11.6719 15.0937C11.6719 14.9793 11.7062 14.9518 11.816 14.9518C12.8683 14.9518 13.9206 14.9518 14.9751 14.9518H15.1215V14.8329C15.1215 13.8057 15.1215 12.774 15.1215 11.7492C15.1274 10.9262 15.3148 10.1146 15.6706 9.37241C16.1301 8.38271 16.9475 7.60378 17.9582 7.19235C18.6492 6.90525 19.3923 6.76428 20.1405 6.7783C21.0029 6.79202 21.8653 6.83091 22.7278 6.86065C22.8879 6.86065 23.048 6.89496 23.2082 6.90182C23.2974 6.90182 23.3271 6.94071 23.3271 7.02993C23.3271 7.54235 23.3271 8.05477 23.3271 8.5649C23.3271 9.16882 23.3271 9.77274 23.3271 10.3767C23.3271 10.4819 23.2974 10.5139 23.1921 10.5116C22.5379 10.5116 21.8814 10.5116 21.2271 10.5116C20.9287 10.5184 20.6316 10.5528 20.3395 10.6146C20.0822 10.6619 19.8463 10.7891 19.6653 10.9779C19.4842 11.1668 19.3672 11.4078 19.3307 11.6669C19.2857 11.893 19.2612 12.1226 19.2575 12.3531C19.2575 13.1904 19.2575 14.0299 19.2575 14.8695C19.2575 14.8946 19.2575 14.9198 19.2575 14.9564H23.0229C23.1807 14.9564 23.183 14.9564 23.1624 15.1074C23.0778 15.7662 22.9885 16.425 22.9039 17.0816C22.8322 17.6321 22.7636 18.1827 22.698 18.7332C22.6729 18.9437 22.6797 18.9437 22.4693 18.9437H19.2644V28.8992C19.2644 28.9793 19.2644 29.0593 19.2644 29.1394L15.1147 29.1371Z" fill="white"></path>
|
|
<path id="Vector_2_Facebook" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
|
</g>
|
|
<defs>
|
|
<clipPath id="clip0_1717_1086">
|
|
<rect width="35" height="35" fill="white"></rect>
|
|
</clipPath>
|
|
</defs>
|
|
</svg>
|
|
</a></li>
|
|
<li class="list-inline-item"><a href="https://www.youtube.com/user/NLMNIH" aria-label="Youtube" target="_blank" rel="noopener noreferrer">
|
|
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
|
<title>Youtube</title>
|
|
<g id="YouTube" clip-path="url(#clip0_1717_1101)">
|
|
<path id="Vector_Youtube" d="M26.2571 11.4791C25.9025 11.1589 25.5709 10.9576 24.228 10.834C22.5512 10.6785 20.2797 10.6556 18.564 10.6533H16.4365C14.7208 10.6533 12.4493 10.6785 10.7725 10.834C9.43196 10.9576 9.09798 11.1589 8.7434 11.4791C7.81464 12.321 7.6202 14.6268 7.59961 16.8938C7.59961 17.3178 7.59961 17.741 7.59961 18.1635C7.62706 20.4121 7.82837 22.686 8.7434 23.521C9.09798 23.8412 9.42967 24.0425 10.7725 24.1661C12.4493 24.3216 14.7208 24.3445 16.4365 24.3468H18.564C20.2797 24.3468 22.5512 24.3216 24.228 24.1661C25.5686 24.0425 25.9025 23.8412 26.2571 23.521C27.1722 22.6929 27.3735 20.451 27.4009 18.2206C27.4009 17.7402 27.4009 17.2599 27.4009 16.7795C27.3735 14.5491 27.1699 12.3072 26.2571 11.4791ZM15.5604 20.5311V14.652L20.561 17.5001L15.5604 20.5311Z" fill="white"></path>
|
|
<path id="Vector_2_Youtube" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
|
</g>
|
|
<defs>
|
|
<clipPath id="clip0_1717_1101">
|
|
<rect width="35" height="35" fill="white"></rect>
|
|
</clipPath>
|
|
</defs>
|
|
</svg>
|
|
</a></li>
|
|
</ul>
|
|
</div>
|
|
<div class="col-lg-3 col-12">
|
|
<p class="address_footer text-white">National Library of Medicine<br />
|
|
<a href="https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/@38.9959508,-77.101021,17z/data=!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb:0x19156f88b27635b8!8m2!3d38.9959508!4d-77.0988323" class="text-white" target="_blank" rel="noopener noreferrer">8600 Rockville Pike<br />
|
|
Bethesda, MD 20894</a></p>
|
|
</div>
|
|
<div class="col-lg-3 col-12 centered-lg">
|
|
<p><a href="https://www.nlm.nih.gov/web_policies.html" class="text-white">Web Policies</a><br />
|
|
<a href="https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office" class="text-white">FOIA</a><br />
|
|
<a href="https://www.hhs.gov/vulnerability-disclosure-policy/index.html" class="text-white" id="vdp">HHS Vulnerability Disclosure</a></p>
|
|
</div>
|
|
<div class="col-lg-3 col-12 centered-lg">
|
|
<p><a class="supportLink text-white" href="https://support.nlm.nih.gov/">Help</a><br />
|
|
<a href="https://www.nlm.nih.gov/accessibility.html" class="text-white">Accessibility</a><br />
|
|
<a href="https://www.nlm.nih.gov/careers/careers.html" class="text-white">Careers</a></p>
|
|
</div>
|
|
</div>
|
|
<div class="row">
|
|
<div class="col-lg-12 centered-lg">
|
|
<nav class="bottom-links">
|
|
<ul class="mt-3">
|
|
<li>
|
|
<a class="text-white" href="//www.nlm.nih.gov/">NLM</a>
|
|
</li>
|
|
<li>
|
|
<a class="text-white" href="https://www.nih.gov/">NIH</a>
|
|
</li>
|
|
<li>
|
|
<a class="text-white" href="https://www.hhs.gov/">HHS</a>
|
|
</li>
|
|
<li>
|
|
<a class="text-white" href="https://www.usa.gov/">USA.gov</a>
|
|
</li>
|
|
</ul>
|
|
</nav>
|
|
</div>
|
|
</div>
|
|
</div>
|
|
</section>
|
|
<script type="text/javascript" src="/portal/portal3rc.fcgi/rlib/js/InstrumentOmnitureBaseJS/InstrumentNCBIConfigJS/InstrumentNCBIBaseJS/InstrumentPageStarterJS.js?v=1"> </script>
|
|
<script type="text/javascript" src="/portal/portal3rc.fcgi/static/js/hfjs2.js"> </script>
|
|
</div>
|
|
</div>
|
|
<!--/.footer-->
|
|
<p class="last-updated small">Last updated: 2024-06-12T20:07:17Z</p>
|
|
</div>
|
|
<!--/.page-->
|
|
</div>
|
|
<!--/.wrap-->
|
|
<span class="PAFAppResources"></span>
|
|
|
|
|
|
</div><!-- /.twelve_col -->
|
|
</div>
|
|
<!-- /.grid -->
|
|
|
|
|
|
|
|
<!-- usually for JS scripts at page bottom -->
|
|
<span class="pagefixtures"></span>
|
|
|
|
|
|
<!-- CE8B5AF87C7FFCB1_0191SID /projects/staticsites/genbank/genbank@2.21 portal105 v4.1.r689238 Tue, Oct 22 2024 16:10:51 -->
|
|
<span id="portal-csrf-token" style="display:none" data-token="CE8B5AF87C7FFCB1_0191SID"></span>
|
|
|
|
<script type="text/javascript" src="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/js/3879255/4121861/1490097/4087685.js" snapshot="genbank"></script></body>
|
|
</html>
|
|
|