publicdata/nih-gov
1
0
Fork 0
Code Issues Pull requests Projects Releases Packages Wiki Activity Actions
nih-gov/www.ncbi.nlm.nih.gov/genbank/asndisc/index.html

792 lines
47 KiB
HTML
Raw Permalink Blame History

<?xml version="1.0" encoding="utf-8"?>
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
<head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<!-- AppResources meta begin -->
<meta name="paf-app-resources" content="" />
<!-- AppResources meta end -->
<!-- TemplateResources meta begin -->
<meta name="paf_template" content="StdNCol" />
<!-- TemplateResources meta end -->
<!-- Page meta begin -->
<!-- Page meta end -->
<!-- Logger begin -->
<meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_app" content="genbank" /><meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_pdid" content="custom-page" />
<!-- Logger end -->
<title>Discrepancy Report</title>
<!-- PageFixtures headcontent begin -->
<meta name="cms-local-nav-url" content="https://cms.ncbi.nlm.nih.gov//genbank/_nav" />
<!-- PageFixtures headcontent end -->
<!-- AppResources external_resources begin -->
<script type="text/javascript" src="/core/jig/1.15.6/js/jig.min.js"></script>
<!-- AppResources external_resources end -->
<!-- Page headcontent begin -->
<meta name="subsite" content="genbank" />
<meta name="path" content="genbank/asndisc" />
<meta name="modified" content="2024-04-26T20:26:57Z" /><meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="cms-edit-aux-url" content="http://cms.ncbi.nlm.nih.gov/node//edit" />
<!-- Page headcontent end -->
<!-- PageFixtures resources begin -->
<link xmlns="http://www.w3.org/1999/xhtml" type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218191/css/4207974/4206132.css" xml:base="http://127.0.0.1/sites/static/header_footer" />
<!-- PageFixtures resources end -->
<link rel="shortcut icon" href="//www.ncbi.nlm.nih.gov/favicon.ico" /><meta name="ncbi_phid" content="CE8ED0EF7D20677100000000012D00FF.m_6" />
<meta name='referrer' content='origin-when-cross-origin'/><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/css/4121862/3974050/3917732/251717/4108189/14534/45193/3534283/4128070/3407145/4005757/4062871.css" /><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/css/3529741/3529739.css" media="print" /></head>
<body class=" col2 custom-page">
<div class="grid">
<div class="col twelve_col nomargin shadow">
<!-- System messages like service outage or JS required; this is handled by the TemplateResources portlet -->
<div class="sysmessages">
<noscript>
<p class="nojs">
<strong>Warning:</strong>
The NCBI web site requires JavaScript to function.
<a href="/guide/browsers/#enablejs" title="Learn how to enable JavaScript" target="_blank">more...</a>
</p>
</noscript>
</div>
<!--/.sysmessage-->
<div class="wrap">
<div class="page">
<div xmlns:xi="http://www.w3.org/2001/XInclude">
<div xmlns="http://www.w3.org/1999/xhtml" id="universal_header" xml:base="http://127.0.0.1/sites/static/header_footer">
<section class="usa-banner">
<div class="usa-accordion">
<header class="usa-banner-header">
<div class="usa-grid usa-banner-inner">
<img src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/favicons/favicon-57.png" alt="U.S. flag" />
<p>An official website of the United States government</p>
<button class="non-usa-accordion-button usa-banner-button" aria-expanded="false" aria-controls="gov-banner-top" type="button">
<span class="usa-banner-button-text">Here's how you know</span>
</button>
</div>
</header>
<div class="usa-banner-content usa-grid usa-accordion-content" id="gov-banner-top" aria-hidden="true">
<div class="usa-banner-guidance-gov usa-width-one-half">
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-dot-gov.svg" alt="Dot gov" />
<div class="usa-media_block-body">
<p>
<strong>The .gov means it's official.</strong>
<br />
Federal government websites often end in .gov or .mil. Before
sharing sensitive information, make sure you're on a federal
government site.
</p>
</div>
</div>
<div class="usa-banner-guidance-ssl usa-width-one-half">
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-https.svg" alt="Https" />
<div class="usa-media_block-body">
<p>
<strong>The site is secure.</strong>
<br />
The <strong>https://</strong> ensures that you are connecting to the
official website and that any information you provide is encrypted
and transmitted securely.
</p>
</div>
</div>
</div>
</div>
</section>
<div class="usa-overlay"></div>
<header class="ncbi-header" role="banner" data-section="Header">
<div class="usa-grid">
<div class="usa-width-one-whole">
<div class="ncbi-header__logo">
<a href="/" class="logo" aria-label="NCBI Logo" data-ga-action="click_image" data-ga-label="NIH NLM Logo">
<img src="https://www.ncbi.nlm.nih.gov/coreutils/nwds/img/logos/AgencyLogo.svg" alt="NIH NLM Logo" />
</a>
</div>
<div class="ncbi-header__account">
<a id="account_login" href="https://account.ncbi.nlm.nih.gov" class="usa-button header-button" style="display:none" data-ga-action="open_menu" data-ga-label="account_menu">Log in</a>
<button id="account_info" class="header-button" style="display:none" aria-controls="account_popup" type="button">
<span class="fa fa-user" aria-hidden="true">
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24" width="20px" height="20px">
<g style="fill: #fff">
<ellipse cx="12" cy="8" rx="5" ry="6"></ellipse>
<path d="M21.8,19.1c-0.9-1.8-2.6-3.3-4.8-4.2c-0.6-0.2-1.3-0.2-1.8,0.1c-1,0.6-2,0.9-3.2,0.9s-2.2-0.3-3.2-0.9 C8.3,14.8,7.6,14.7,7,15c-2.2,0.9-3.9,2.4-4.8,4.2C1.5,20.5,2.6,22,4.1,22h15.8C21.4,22,22.5,20.5,21.8,19.1z"></path>
</g>
</svg>
</span>
<span class="username desktop-only" aria-hidden="true" id="uname_short"></span>
<span class="sr-only">Show account info</span>
</button>
</div>
<div class="ncbi-popup-anchor">
<div class="ncbi-popup account-popup" id="account_popup" aria-hidden="true">
<div class="ncbi-popup-head">
<button class="ncbi-close-button" data-ga-action="close_menu" data-ga-label="account_menu" type="button">
<span class="fa fa-times">
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 48 48" width="24px" height="24px">
<path d="M38 12.83l-2.83-2.83-11.17 11.17-11.17-11.17-2.83 2.83 11.17 11.17-11.17 11.17 2.83 2.83 11.17-11.17 11.17 11.17 2.83-2.83-11.17-11.17z"></path>
</svg>
</span>
<span class="usa-sr-only">Close</span></button>
<h4>Account</h4>
</div>
<div class="account-user-info">
Logged in as:<br />
<b><span class="username" id="uname_long">username</span></b>
</div>
<div class="account-links">
<ul class="usa-unstyled-list">
<li><a id="account_myncbi" href="/myncbi/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_myncbi">Dashboard</a></li>
<li><a id="account_pubs" href="/myncbi/collections/bibliography/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_pubs">Publications</a></li>
<li><a id="account_settings" href="/account/settings/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_settings">Account settings</a></li>
<li><a id="account_logout" href="/account/signout/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_logout">Log out</a></li>
</ul>
</div>
</div>
</div>
</div>
</div>
</header>
<div role="navigation" aria-label="access keys">
<a id="nws_header_accesskey_0" href="https://www.ncbi.nlm.nih.gov/guide/browsers/#ncbi_accesskeys" class="usa-sr-only" accesskey="0" tabindex="-1">Access keys</a>
<a id="nws_header_accesskey_1" href="https://www.ncbi.nlm.nih.gov" class="usa-sr-only" accesskey="1" tabindex="-1">NCBI Homepage</a>
<a id="nws_header_accesskey_2" href="/myncbi/" class="set-base-url usa-sr-only" accesskey="2" tabindex="-1">MyNCBI Homepage</a>
<a id="nws_header_accesskey_3" href="#maincontent" class="usa-sr-only" accesskey="3" tabindex="-1">Main Content</a>
<a id="nws_header_accesskey_4" href="#" class="usa-sr-only" accesskey="4" tabindex="-1">Main Navigation</a>
</div>
<section data-section="Alerts">
<div class="ncbi-alerts-placeholder"></div>
</section>
</div>
</div>
<!--/.header-->
<div class="header">
<div class="res_logo"><h1 class="res_name"><a href="/genbank/" title="GenBank home">GenBank</a></h1><h2 class="res_tagline">Public nucleic acid sequence repository</h2></div>
<div class="search"><form method="get" action="/nuccore/"><div class="search_form"><label for="database" class="offscreen_noflow">Search database</label><select id="database"><optgroup label="Recent"><option value="nuccore" selected="selected">Nucleotide</option><option value="gquery">All Databases</option><option value="books">Books</option><option value="sra" class="last">SRA</option></optgroup><optgroup label="All"><option value="gquery">All Databases</option><option value="assembly">Assembly</option><option value="biocollections">Biocollections</option><option value="bioproject">BioProject</option><option value="biosample">BioSample</option><option value="books">Books</option><option value="clinvar">ClinVar</option><option value="cdd">Conserved Domains</option><option value="gap">dbGaP</option><option value="dbvar">dbVar</option><option value="gene">Gene</option><option value="genome">Genome</option><option value="gds">GEO DataSets</option><option value="geoprofiles">GEO Profiles</option><option value="gtr">GTR</option><option value="ipg">Identical Protein Groups</option><option value="medgen">MedGen</option><option value="mesh">MeSH</option><option value="nlmcatalog">NLM Catalog</option><option value="nuccore">Nucleotide</option><option value="omim">OMIM</option><option value="pmc">PMC</option><option value="protein">Protein</option><option value="proteinclusters">Protein Clusters</option><option value="protfam">Protein Family Models</option><option value="pcassay">PubChem BioAssay</option><option value="pccompound">PubChem Compound</option><option value="pcsubstance">PubChem Substance</option><option value="pubmed">PubMed</option><option value="snp">SNP</option><option value="sra">SRA</option><option value="structure">Structure</option><option value="taxonomy">Taxonomy</option><option value="toolkit">ToolKit</option><option value="toolkitall">ToolKitAll</option><option value="toolkitbookgh">ToolKitBookgh</option></optgroup></select><div class="nowrap"><label for="term" class="offscreen_noflow" accesskey="/">Search term</label><div class="nowrap"><input type="text" name="term" id="term" title="Search Nucleotide" value="" class="jig-ncbiclearbutton jig-ncbiautocomplete" data-jigconfig="isEnabled:false,disableUrl:'NcbiSearchBarAutoComplCtrl'" autocomplete="off" data-sbconfig="ds:'no',pjs:'no',afs:'yes'" /></div><button id="search" type="submit" class="button_search nowrap" cmd="go">Search</button></div></div></form></div>
</div>
<div class="nav_and_browser">
<div class="localnav"><ul class="jig-ncbilocalnav">
<li><a href="#">GenBank</a><ul>
<li><a href="/genbank/">About GenBank</a></li>
<li><a href="/genbank/submit_types">Submission Types</a></li>
<li><a href="/genbank/submit">Submission Tools</a></li>
<li><a href="/genbank/update">Update GenBank Records</a></li>
<li><a href="/nuccore/">Search</a></li>
<li><a href="/BLAST/Blast.cgi?CMD=Web&amp;PAGETYPE=BLASTHome">BLAST</a></li>
<li><a href="/genbank/statistics">Statistics</a></li>
<li><a href="/genbank/samplerecord/">Sample Record</a></li>
<li><a href="/genbank/sequencerevisionhistory/">Revision History</a></li>
<li><a href="/genbank/sequenceids/">Sequence IDs</a></li>
</ul>
</li>
<li><a href="#">Submit</a><ul>
<li><a href="/genbank/submit">Submission Tools</a></li>
<li><a href="/genbank/submit_types">Submission Types</a></li>
<li><a href="/WebSub/?tool=genbank">BankIt</a></li>
<li><a href="/genbank/table2asn">table2asn</a></li>
<li><a href="https://www.ncbi.nlm.nih.gov/sra/docs/sequence-data-processing">Sequence Data Processing</a></li>
</ul>
</li>
<li><a href="#">Genomes</a><ul>
<li><a href="/genbank/genomesubmit">Complete Genome Submission Guide</a></li>
<li><a href="/genbank/genomesubmit_annotation">Prokaryotic Genome Annotation Guide</a></li>
<li><a href="/genbank/eukaryotic_genome_submission_annotation">Eukaryotic Genome Annotation Guide</a></li>
<li><a href="/genbank/examples.wgs">Annotation Examples</a></li>
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/wgs/">Genome Submission Portal</a></li>
</ul>
</li>
<li><a title="Whole Genome Shotgun sequences and submissions" href="#">WGS</a><ul>
<li><a href="/genbank/wgs">About WGS</a></li>
<li><a href="/Traces/wgs">WGS Project List</a></li>
<li><a href="/genbank/wgs.submit">WGS Submission Guide</a></li>
<li><a href="/genbank/wgsfaq/">FAQ</a></li>
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/wgs/">Genome Submission Portal</a></li>
<li><a href="/genbank/eukaryotic_genome_submission_annotation">Eukaryotic Annotation Guide</a></li>
<li><a href="/genbank/genomesubmit_annotation">Prokaryotic Annotation Guide</a></li>
<li><a href="/genbank/asndisc">Discrepancy Report</a></li>
<li><a href="/assembly/agp/AGP_Specification/">AGP format</a></li>
</ul>
</li>
<li><a href="#">Metagenomes</a><ul>
<li><a href="/genbank/metagenome">About Metagenomes</a></li>
<li><a href="/genbank/structuredcomment">Structured Comment</a></li>
</ul>
</li>
<li><a href="#">TPA</a><ul>
<li><a href="/genbank/TPA">About TPA</a></li>
<li><a href="/genbank/tpafaq">FAQ</a></li>
<li><a href="/genbank/TPA-Exp">TPA-Exp</a></li>
<li><a href="/genbank/TPA-Inf">TPA-Inf</a></li>
</ul>
</li>
<li><a href="#">TSA</a><ul>
<li><a href="/genbank/TSA">About TSA</a></li>
<li><a href="/genbank/TSAguide">TSA Submission Guide</a></li>
<li><a href="/genbank/TSAfaq">FAQ</a></li>
</ul>
</li>
<li><a href="#">INSDC</a><ul>
<li><a href="/genbank/collab">About INSDC</a></li>
<li><a href="/genbank/collab/country">Geographic Location Name List</a></li>
<li><a href="/genbank/collab/db_xref">db_xref List</a></li>
<li><a href="http://www.insdc.org/documents/feature_table.html">Feature Table</a></li>
</ul>
</li>
<li><a href="#">Documentation</a><ul>
<li><a href="https://www.ncbi.nlm.nih.gov/sra/docs/sequence-data-processing/">Sequence Data Processing</a></li>
<li><a href="/genbank/submission_brokers">Submission Brokers</a></li>
<li><a href="/genbank/acc_prefix">Accession Number Prefixes</a></li>
<li><a href="/genbank/organelle_submit/">Organelle Submission Guide</a></li>
<li><a href="/genbank/monkeypox_submission/">Monkeypox Submission Guide</a></li>
<li><a href="/genbank/validation/">Common Submission Errors</a> </li>
<li><a href="/genbank/sequencecheck/">Ribosomal Submission Errors</a></li>
<li><a href="/genbank/sequencecheck/virus">Common Sequence Errors</a></li>
<li><a href="https://support.nlm.nih.gov/knowledgebase/category/?id=CAT-01240">Submission FAQs</a></li>
</ul>
</li>
<li><a href="#">Other</a><ul>
<li><a href="/genbank/htgs">About HTGs</a></li>
<li><a href="/genbank/dbest">About EST</a></li>
<li><a href="/genbank/dbgss">About GSS</a></li>
<li><a href="/genbank/tls">About TLS</a></li>
<li><a href="/genbank/tlsguide">Submit TLS</a></li>
</ul>
</li>
</ul></div>
</div>
<!-- was itemctrl -->
<div class="container">
<div id="maincontent" class="content col twelve_col last">
<div class="col1">
<h1 id="discrepancy-report">Discrepancy Report</h1>
<h3 id="introduction">Introduction</h3>
<p>The Discrepancy Report is an evaluation of a single or multiple ASN.1
files, looking for suspicious annotation or annotation discrepancies
that NCBI staff has noticed commonly occur in genome submissions, both
complete and incomplete (WGS). A few of the problems that this
function was written to find include inconsistent locus_tag prefixes,
missing gene features, and suspect product
names. The function is available as an argument for table2asn or with the command-line program asndisc.
Note that the test names were improved in 2019, so those in the output from the newer tools differ slightly from those produced by tbl2asn.</p>
<p>Categories prefaced with FATAL should almost always be corrected before submitting to GenBank to avoid processing delays. (The exceptions are FATALs about bacteria when the genome is not bacterial.) Some of the categories are informational. Reports that are not flagged as fatal should be examined to determine if they represent annotation artifacts that need to be corrected or if they are acceptable due to the biology of the genome. </p>
<p>If you have questions about the Discrepancy Report, please contact us by email at <a href="mailto:genomes@ncbi.nlm.nih.gov">genomes@ncbi.nlm.nih.gov</a> prior to sending us your submission.</p>
<p>For more information about annotation requirements, be sure to read the appropriate annotation guidelines:</p>
<ul>
<li><a href="/genbank/genomesubmit_annotation">Prokaryotic Genome Guidelines</a></li>
<li><a href="/genbank/eukaryotic_genome_submission">Eukaryotic Genome Guidelines</a></li>
</ul>
<p>You may be interested to know that NCBI has a publicly available
<a href="/genome/annotation_prok/">Prokaryotic Genomes Annotation Pipeline</a>, which you can <a href="/genbank/genomesubmit/#pgap">request during submission</a> of the genome(s) to GenBank or <a href="/genbank/genomesubmit/#run_pgap">run yourself</a>.</p>
<h3 id="table-of-contents">Table of Contents</h3>
<ol>
<li>
<p><a href="#Usingtbl2asn">Using tbl2asn</a> or <a href="#Usingtable2asn">table2asn</a></p>
</li>
<li>
<p><a href="#Usingasndisc">Using asndisc</a></p>
</li>
<li>
<p><a href="#evaluating_the_output">Evaluating the output</a></p>
<ul>
<li><a href="#fatal">Fatal reports</a></li>
</ul>
</li>
<li>
<p><a href="#common_reports">Common Reports</a></p>
<ul>
<li><a href="/genbank/new_asndisc_examples/">From table2asn and Submission Portal</a></li>
<li><a href="/genbank/asndisc.examples">From tbl2asn</a></li>
</ul>
</li>
</ol>
<h2 id="Usingtable2asn">Using table2asn</h2>
<p>To run the discrepancy report using table2asn, include the argument
-Z. When run on a single file, the output will be a file with the same base name as the
input file but with the suffix ".dr". When run on a directory, the output will be a file
with the suffix ".dr" and the basename of the directory on which it was run. For example, a typical command
when there is no annotation or when the annotation is in .tbl files would look like this:</p>
<p><strong>table2asn -indir path_to_fsa_files -t template -M n -Z</strong></p>
<p>and would produce an output file named "path_to_fsa_files.dr". </p>
<p>For more information, see the <a href="/genbank/genomes_gff/#run">table2asn instructions</a>. Examine the contents of the ".dr" output file: <a href="#evaluating_the_output">Evaluating the output</a></p>
<h2 id="Usingtbl2asn">Using tbl2asn</h2>
<p>To run the discrepancy report using tbl2asn, include the argument -Z <em>with the name of the output file</em>. For example, a typical command would look like this:</p>
<p><strong>tbl2asn -p path_to_fsa_files -t template -M n -Z discrep</strong></p>
<p>For more information, see the <a href="/genbank/tbl2asn2">tbl2asn instructions</a> . Examine the contents of the output file, "discrep": <a href="#evaluating_the_output">Evaluating the output</a></p>
<h2 id="Usingasndisc">Using asndisc</h2>
<p>The commandline program asndisc is available by anonymous <a href="https://ftp.ncbi.nih.gov/toolbox/ncbi_tools/converters/by_program/asndisc/">FTP</a> . Copy the right version for your platform, then uncompress the file, rename it to "asndisc", and set the permissions, as necessary for the platform.</p>
<p>asndisc examines all the files with a common suffix in a directory and collates all the discrepancies into an output file. The standard usage runs all of the tests, but specific tests can be enabled or disabled. In addition, expanded reports of particular tests can be generated. Running "asndisc -help" provides the list of arguments and tests. We are actively updating asndisc to reflect what we see in submitted annotation. Please download the most recent version to be sure all of the latest tests are included. Note that the format of the version changed in the fall of 2019.</p>
<p>This is the recommended usage for ASN files that were created using table2asn:</p>
<pre><code>asndisc -P u -indir path_to_files -x .file_suffix -o output_file
-indir Path to Files [String]
-x File Selection Substring [String] (default = '.sqn')
-o Single Output File [File Out]
-X Expand Report Categories (comma-delimited list of test names or ALL)
-P u Run 'genome submitter' set of tests and include FATAL tags in output
</code></pre>
<p>For example the following commandline will run asndisc on all the .sqn files in the directory named DIR and will put the output in a file named discrep:</p>
<p><strong>asndisc -P u -indir DIR/ -x .sqn -o discrep</strong></p>
<p>NOTE: The output file will have the same content as the output file from the table2asn commandline "table2asn -indir path_to_fsa_files -t template -M n -Z" </p>
<p>Examine the contents of the output file, "discrep": <a href="#evaluating_the_output">Evaluating the output</a></p>
<h2 id="evaluating_the_output">Evaluating the output</h2>
<p>In the output file, test results are sorted by category. The top-level categories will be listed in the summary at the top of the file. Some of the reports also have subcategories that contain more descriptive information. </p>
<p>The Discrepancy Report is something of a blunt instrument that reports everything that fails its tests; it does not consider whether those failures are real problems or just a reflection of the biology. Look at the problematic features in the output file and examine those features in the .sqn files to determine whether the problems are real and need to be corrected, or can be ignored because the situation reflects the biology.</p>
<h3 id="fatal">Fatal reports</h3>
<p>LIST of FATAL categories:</p>
<ul>
<li>BACTERIAL_PARTIAL_NONEXTENDABLE_PROBLEMS *</li>
<li>BAD_LOCUS_TAG_FORMAT</li>
<li>CITSUBAFFIL_CONFLICT</li>
<li>CONTAINED_CDS</li>
<li>EC_NUMBER_ON_UNKNOWN_PROTEIN</li>
<li>EUKARYOTE_SHOULD_HAVE_MRNA</li>
<li>INCONSISTENT_PROTEIN_ID</li>
<li>MAP_CHROMOSOME_CONFLICT</li>
<li>MICROSATELLITE_REPEAT_TYPE</li>
<li>MISSING_AFFIL</li>
<li>MISSING_GENES</li>
<li>MISSING_GENOMEASSEMBLY_COMMENTS</li>
<li>MISSING_PROTEIN_ID</li>
<li>MRNA_SHOULD_HAVE_PROTEIN_TRANSCRIPT_IDS</li>
<li>N_RUNS</li>
<li>OVERLAPPING_RRNAS</li>
<li>PARTIAL_PROBLEMS</li>
<li>PSEUDO_MISMATCH</li>
<li>RBS_WITHOUT_GENE</li>
<li>RIBOSOMAL_SLIPPAGE</li>
<li>RNA_CDS_OVERLAP (when coding regions are completely contained in
RNAs)</li>
<li>RRNA_NAME_CONFLICTS</li>
<li>SHORT_RRNA</li>
<li>SHOW_HYPOTHETICAL_CDS_HAVING_GENE_NAME</li>
<li>SOURCE_QUALS (eg, when taxname differs)</li>
<li>SUSPECT_PRODUCT_NAMES<ul>
<li>"Remove organism from product name" category</li>
<li>"Possible parsing error or incorrect formatting; remove
inappropriate symbols\" category</li>
</ul>
</li>
<li>TERMINAL_NS</li>
<li>TITLE_AUTHOR_CONFLICT</li>
<li>UNCULTURED_NOTES</li>
<li>UNPUB_PUB_WITHOUT_TITLE</li>
</ul>
<p>* <strong>These are only FATAL for prokaryotes</strong>. However, the report appears with the FATAL tag when the files do not include the full taxonomy lookup, which often does not happen until processing here. We have kept them as FATAL so that submitters see the error and can decide whether it is relevant for that particular submission or not.</p>
<ul>
<li>BACTERIAL_JOINED_FEATURES_NO_EXCEPTION</li>
<li>BACTERIAL_PARTIAL_NONEXTENDABLE_PROBLEMS</li>
<li>BACTERIA_SHOULD_NOT_HAVE_MRNA</li>
</ul>
<p>These three categories are suspicious but weren't marked as FATAL because the situation is sometimes valid. They will always require confirmation from the submitter that they are biologically correct. These are categories that find CDS and/or RNAs overlapping or contained within each other:</p>
<ul>
<li>OVERLAPPING_CDS</li>
<li>CONTAINED_CDS</li>
<li>RNA_CDS_OVERLAP</li>
</ul>
<h3 id="common_reports">Common reports</h3>
<p>The discrepancy report test names were improved in the newest asndisc (2019). We hope that the newer names are easier to understand. Here are explanations of some common discrepancy report categories, for the newer and older test names:</p>
<ul>
<li><a href="/genbank/new_asndisc_examples">Newer test names</a>, from table2asn and Submission Portal</li>
<li><a href="/genbank/asndisc.examples">Older test names</a>, from retired tbl2asn</li>
</ul>
<p>Here is a summary of the analysis of a submission, performed with the default settings of asndisc:</p>
<h3 id="summary">Summary</h3>
<ul>
<li>SOURCE_QUALS:strain (all present, all same)</li>
<li>SOURCE_QUALS:taxname (all present, all same)</li>
<li>FEATURE_COUNT:gene: 15712 present</li>
<li>FEATURE_COUNT:CDS: 15708 present</li>
<li>FEATURE_COUNT:mRNA: 15708 present</li>
<li>FEATURE_COUNT:misc_RNA: 1 present</li>
<li>FEATURE_COUNT:rRNA: 3 present</li>
<li>JOINED_FEATURES:14502 features have joined locations</li>
<li>COUNT_NUCLEOTIDES:209 nucleotide Bioseqs are present</li>
<li>EC_NUMBER_NOTE:2 features have EC numbers in notes or products.</li>
<li>FEATURE_LOCATION_CONFLICT:13007 features have inconsistent gene
locations.</li>
<li>CONTAINED_CDS:3 coding regions are completely contained in another
coding region.</li>
<li>SUSPECT_PRODUCT_NAMES:25 product_names contain 'suspect phrase
or characters'<ul>
<li>6 product names contain 'Brackets or parenthesis [] ()'</li>
<li>4 product names contain 'Mitochondrial'</li>
<li>4 product names contain 'N-term'</li>
<li>2 product names contain 'Related to'</li>
<li>2 product names contain 'Similar to'</li>
<li>2 product names contain 'gene'</li>
<li>2 product names contain 'partial'</li>
<li>2 product names contain 'similar'</li>
<li>1 product names end with like</li>
</ul>
</li>
<li>SHORT_INTRON:221 introns are shorter than 10 nt</li>
<li>NO_ANNOTATION:12 bioseqs have no features</li>
<li>UNUSUAL_MISC_RNA:1 unexpected misc_RNA features found.</li>
<li>FATAL: OVERLAPPING_RRNAS:3 rRNA features overlap another rRNA
feature.</li>
<li>FATAL: SHOW_HYPOTHETICAL_CDS_HAVING_GENE_NAME:1 hypothetical
coding regions have a gene name</li>
<li>QUALITY_SCORES:Quality scores are missing on all sequences.</li>
</ul>
<p>Since this was a eukaryotic organism with introns, the "features have joined locations" is expected. Similarly, since the submitters have UTR information for some mRNAs, those mRNAs (and, therefore, their genes) will extend beyond their CDS, generating "features have inconsistent gene locations" reports. However, the other reports need to be investigated to determine whether they indicate a real problem with the annotation. For example, EC numbers need to be fielded in the EC_number qualifier, unless they are within a note about similarity to another protein. However, since that function looks for #.#.#.# in product names and notes, non-EC numbers that have that format will appear in that report. Similarly, short introns may be an indication that artificial introns were inserted to correct a frameshift, which is not biologically valid.</p>
<p>The discrepancy report also looks for common errors in product names. Review the names and fix those that are incorrect. Since this is a eukaryote, it is possible that some of these are nuclear genes encoding organellar proteins, so perhaps the mitochondrial reports should be ignored. In contrast, no product name should contain the word 'partial'. Keep in mind that the discrepancy report will not catch every bad product name. See the product name guidelines in the <a href="/genbank/genomesubmit_annotation/#CDS">Prokaryotic</a> and <a href="/genbank/eukaryotic_genome_submission_annotation/#CDS">Eukaryotic</a> annotation guidelines for recommended and inappropriate product name formats.</p>
<p>If a subset of the submission is not annotated, as in this sample (reported as "NO_ANNOTATION:12 bioseqs have no features"), please let us know when you submit. We occasionally find sequences with missing annotation caused by incorrectly formatted table headers. Therefore, we will ask you to verify that unannotated records are expected, particularly if they are large sequences.</p>
<p>With regards to the QUALITY_SCORES output, we encourage all submitters to provide quality scores for contig sequences, when possible, for example when all the sequencing was done by Sanger-style sequencing on an ABI machine. We realize that for NextGen sequencing technologies (e.g., 454 or Illumina) the normalization of quality scores across different platforms is still being discussed, so we are not currently expecting quality scores on those submissions. In addition, scaffold objects do not contain quality scores. For more information on how to include quality scores, see the <a href="/genbank/genomesubmit">genome submission instructions</a> .</p>
<p>After you've run the Discrepancy Report and fixed the problem annotation, let us know when you submit your genome about reports that you think can be ignored and why. If you are not certain whether a particular test is important for your genome, please ask us.</p>
</div>
<!--/.col1-->
<div class="col2">
<div class="rightnav">
<h2 id="genome-resources">Genome Resources</h2>
<ul>
<li><a href="/genbank/wgs/">About WGS</a></li>
<li><a href="https://www.ncbi.nlm.nih.gov/Traces/wgs/?view=wgs">WGS Browser</a></li>
<li><a href="/genbank/genomesubmit/">Genome Submission Guide</a></li>
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/genome/">Genome Submission Portal</a></li>
<li><a href="/genbank/wgs_update/">Update Genome Records</a></li>
<li><a href="/genbank/wgsfaq/">FAQ</a></li>
<li><a href="/genbank/table2asn">table2asn</a></li>
<li><a href="/genbank/diploid_haps">Submitting Multiple Haplotype Assemblies</a></li>
<li><a href="/WebSub/template.cgi/">Create Submission Template</a></li>
<li><a href="/genbank/eukaryotic_genome_submission/">Eukaryotic Annotation Guide</a></li>
<li><a href="/genbank/genomesubmit_annotation/">Prokaryotic Annotation Guide</a></li>
<li><a href="/genbank/examples.wgs/">Annotation Example Files</a></li>
<li><a href="/genbank/genomes_gff">Annotating Genomes with GFF3 or GTF files</a></li>
<li><a href="/genbank/genome_validation">Validation Error Explanations for Genomes</a></li>
<li><a href="/genbank/asndisc/">Discrepancy Report</a></li>
<li><a href="https://www.ncbi.nlm.nih.gov/genome/annotation_prok/">NCBI Prokaryotic Genome Annotation Pipeline</a></li>
<li><a href="https://www.ncbi.nlm.nih.gov/assembly/agp/AGP_Specification/">AGP Format</a></li>
<li><a href="/genbank/metagenome/">Metagenome Submission Guide</a></li>
<li><a href="/genbank/structuredcomment/">Structured Comment</a></li>
<li><a href="/bioproject/">BioProject</a></li>
<li><a href="/biosample/">BioSample</a></li>
</ul>
</div>
</div>
<!--/.col2-->
<div class="col3">
</div>
<!--/.col3-->
<div class="col4">
</div>
<!--/.col4-->
<div class="col5">
</div>
<div class="col6">
</div>
<div class="col7">
</div>
<div class="col8">
</div>
<div class="col9">
</div>
</div><!--/.content-->
</div><!--/.container-->
<div id="NCBIFooter_dynamic">
<div class="breadcrumbs">You are here:
<span id="breadcrumb_text"><a href="/guide/">NCBI</a></span></div>
<a id="help-desk-link" class="help_desk" href="https://support.ncbi.nlm.nih.gov/ics/support/default.asp?Time=2025-03-12T19:12:08-04:00&amp;Snapshot=%2Fprojects%2Fstaticsites%2Fgenbank%2Fgenbank@2.21&amp;Host=portal107&amp;ncbi_phid=CE8ED0EF7D20677100000000012D00FF&amp;ncbi_session=CE8B5AF87C7FFCB1_0191SID&amp;from=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fgenbank%2Fasndisc%2F&amp;Ncbi_App=genbank&amp;Page=custom-page&amp;style=classic&amp;deptID=28049" target="_blank">Support Center</a>
<noscript><img alt="" src="/stat?jsdisabled=true&amp;ncbi_app=genbank&amp;ncbi_db=&amp;ncbi_pdid=custom-page&amp;ncbi_phid=CE8ED0EF7D20677100000000012D00FF" /></noscript>
</div>
<div xmlns:xi="http://www.w3.org/2001/XInclude">
<div xmlns="http://www.w3.org/1999/xhtml" class="footer" id="footer" xml:base="http://127.0.0.1/sites/static/header_footer">
<section class="icon-section">
<div id="icon-section-header" class="icon-section_header">Follow NCBI</div>
<div class="grid-container container">
<div class="icon-section_container">
<a class="footer-icon" id="footer_twitter" href="https://twitter.com/ncbi" aria-label="Twitter">
<svg xmlns="http://www.w3.org/2000/svg" width="40" height="40" viewBox="0 0 40 40" fill="none">
<title>Twitter</title>
<g id="twitterx1008">
<path id="path1008" d="M6.06736 7L16.8778 20.8991L6.00001 32.2H10.2L18.6 23.1L25.668 32.2H34L22.8 17.5L31.9 7H28.4L20.7 15.4L14.401 7H6.06898H6.06736ZM9.66753 8.73423H12.9327L29.7327 30.4658H26.5697L9.66753 8.73423Z" fill="#5B616B"></path>
</g>
</svg>
</a>
<a class="footer-icon" id="footer_facebook" href="https://www.facebook.com/ncbi.nlm" aria-label="Facebook"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
<title>Facebook</title>
<path class="cls-11" d="M210.5,115.12H171.74V97.82c0-8.14,5.39-10,9.19-10h27.14V52l-39.32-.12c-35.66,0-42.42,26.68-42.42,43.77v19.48H99.09v36.32h27.24v109h45.41v-109h35Z">
</path>
</svg></a>
<a class="footer-icon" id="footer_linkedin" href="https://www.linkedin.com/company/ncbinlm" aria-label="LinkedIn"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
<title>LinkedIn</title>
<path class="cls-11" d="M101.64,243.37H57.79v-114h43.85Zm-22-131.54h-.26c-13.25,0-21.82-10.36-21.82-21.76,0-11.65,8.84-21.15,22.33-21.15S101.7,78.72,102,90.38C102,101.77,93.4,111.83,79.63,111.83Zm100.93,52.61A17.54,17.54,0,0,0,163,182v61.39H119.18s.51-105.23,0-114H163v13a54.33,54.33,0,0,1,34.54-12.66c26,0,44.39,18.8,44.39,55.29v58.35H198.1V182A17.54,17.54,0,0,0,180.56,164.44Z">
</path>
</svg></a>
<a class="footer-icon" id="footer_github" href="https://github.com/ncbi" aria-label="GitHub"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
<defs>
<style>
.cls-11,
.cls-12 {
fill: #737373;
}
.cls-11 {
fill-rule: evenodd;
}
</style>
</defs>
<title>GitHub</title>
<path class="cls-11" d="M151.36,47.28a105.76,105.76,0,0,0-33.43,206.1c5.28,1,7.22-2.3,7.22-5.09,0-2.52-.09-10.85-.14-19.69-29.42,6.4-35.63-12.48-35.63-12.48-4.81-12.22-11.74-15.47-11.74-15.47-9.59-6.56.73-6.43.73-6.43,10.61.75,16.21,10.9,16.21,10.9,9.43,16.17,24.73,11.49,30.77,8.79,1-6.83,3.69-11.5,6.71-14.14C108.57,197.1,83.88,188,83.88,147.51a40.92,40.92,0,0,1,10.9-28.39c-1.1-2.66-4.72-13.42,1-28,0,0,8.88-2.84,29.09,10.84a100.26,100.26,0,0,1,53,0C198,88.3,206.9,91.14,206.9,91.14c5.76,14.56,2.14,25.32,1,28a40.87,40.87,0,0,1,10.89,28.39c0,40.62-24.74,49.56-48.29,52.18,3.79,3.28,7.17,9.71,7.17,19.58,0,14.15-.12,25.54-.12,29,0,2.82,1.9,6.11,7.26,5.07A105.76,105.76,0,0,0,151.36,47.28Z">
</path>
<path class="cls-12" d="M85.66,199.12c-.23.52-1.06.68-1.81.32s-1.2-1.06-.95-1.59,1.06-.69,1.82-.33,1.21,1.07.94,1.6Zm-1.3-1">
</path>
<path class="cls-12" d="M90,203.89c-.51.47-1.49.25-2.16-.49a1.61,1.61,0,0,1-.31-2.19c.52-.47,1.47-.25,2.17.49s.82,1.72.3,2.19Zm-1-1.08">
</path>
<path class="cls-12" d="M94.12,210c-.65.46-1.71,0-2.37-.91s-.64-2.07,0-2.52,1.7,0,2.36.89.65,2.08,0,2.54Zm0,0"></path>
<path class="cls-12" d="M99.83,215.87c-.58.64-1.82.47-2.72-.41s-1.18-2.06-.6-2.7,1.83-.46,2.74.41,1.2,2.07.58,2.7Zm0,0">
</path>
<path class="cls-12" d="M107.71,219.29c-.26.82-1.45,1.2-2.64.85s-2-1.34-1.74-2.17,1.44-1.23,2.65-.85,2,1.32,1.73,2.17Zm0,0">
</path>
<path class="cls-12" d="M116.36,219.92c0,.87-1,1.59-2.24,1.61s-2.29-.68-2.3-1.54,1-1.59,2.26-1.61,2.28.67,2.28,1.54Zm0,0">
</path>
<path class="cls-12" d="M124.42,218.55c.15.85-.73,1.72-2,1.95s-2.37-.3-2.52-1.14.73-1.75,2-2,2.37.29,2.53,1.16Zm0,0"></path>
</svg></a>
<a class="footer-icon" id="footer_blog" href="https://ncbiinsights.ncbi.nlm.nih.gov/" aria-label="Blog">
<svg xmlns="http://www.w3.org/2000/svg" id="Layer_1" data-name="Layer 1" viewBox="0 0 40 40">
<defs><style>.cls-1{fill:#737373;}</style></defs>
<title>NCBI Insights Blog</title>
<path class="cls-1" d="M14,30a4,4,0,1,1-4-4,4,4,0,0,1,4,4Zm11,3A19,19,0,0,0,7.05,15a1,1,0,0,0-1,1v3a1,1,0,0,0,.93,1A14,14,0,0,1,20,33.07,1,1,0,0,0,21,34h3a1,1,0,0,0,1-1Zm9,0A28,28,0,0,0,7,6,1,1,0,0,0,6,7v3a1,1,0,0,0,1,1A23,23,0,0,1,29,33a1,1,0,0,0,1,1h3A1,1,0,0,0,34,33Z"></path>
</svg>
</a>
</div>
</div>
</section>
<section class="container-fluid bg-primary">
<div class="container pt-5">
<div class="row mt-3">
<div class="col-lg-3 col-12">
<p><a class="text-white" href="https://www.nlm.nih.gov/socialmedia/index.html">Connect with NLM</a></p>
<ul class="list-inline social_media">
<li class="list-inline-item"><a href="https://twitter.com/NLM_NIH" aria-label="Twitter" target="_blank" rel="noopener noreferrer">
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
<title>Twitter</title>
<g id="twitterx1009" clip-path="url(#clip0_65276_3946)">
<path id="Vector_Twitter" d="M17.5006 34.6565C26.9761 34.6565 34.6575 26.9751 34.6575 17.4996C34.6575 8.02416 26.9761 0.342773 17.5006 0.342773C8.02514 0.342773 0.34375 8.02416 0.34375 17.4996C0.34375 26.9751 8.02514 34.6565 17.5006 34.6565Z" fill="#205493" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
<path id="path1009" d="M8.54811 8.5L16.2698 18.4279L8.50001 26.5H11.5L17.5 20L22.5486 26.5H28.5L20.5 16L27 8.5H24.5L19 14.5L14.5007 8.5H8.54927H8.54811ZM11.1197 9.73873H13.4519L25.4519 25.2613H23.1926L11.1197 9.73873Z" fill="white"></path>
</g>
<defs>
<clipPath id="clip0_65276_3946">
<rect width="35" height="35" fill="white"></rect>
</clipPath>
</defs>
</svg>
</a></li>
<li class="list-inline-item"><a href="https://www.facebook.com/nationallibraryofmedicine" aria-label="Facebook" rel="noopener noreferrer" target="_blank">
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
<title>Facebook</title>
<g id="Facebook" clip-path="url(#clip0_1717_1086)">
<path id="Vector_Facebook" d="M15.1147 29.1371C15.1147 29.0822 15.1147 29.0296 15.1147 28.9747V18.9414H11.8183C11.6719 18.9414 11.6719 18.9414 11.6719 18.8018C11.6719 17.5642 11.6719 16.3289 11.6719 15.0937C11.6719 14.9793 11.7062 14.9518 11.816 14.9518C12.8683 14.9518 13.9206 14.9518 14.9751 14.9518H15.1215V14.8329C15.1215 13.8057 15.1215 12.774 15.1215 11.7492C15.1274 10.9262 15.3148 10.1146 15.6706 9.37241C16.1301 8.38271 16.9475 7.60378 17.9582 7.19235C18.6492 6.90525 19.3923 6.76428 20.1405 6.7783C21.0029 6.79202 21.8653 6.83091 22.7278 6.86065C22.8879 6.86065 23.048 6.89496 23.2082 6.90182C23.2974 6.90182 23.3271 6.94071 23.3271 7.02993C23.3271 7.54235 23.3271 8.05477 23.3271 8.5649C23.3271 9.16882 23.3271 9.77274 23.3271 10.3767C23.3271 10.4819 23.2974 10.5139 23.1921 10.5116C22.5379 10.5116 21.8814 10.5116 21.2271 10.5116C20.9287 10.5184 20.6316 10.5528 20.3395 10.6146C20.0822 10.6619 19.8463 10.7891 19.6653 10.9779C19.4842 11.1668 19.3672 11.4078 19.3307 11.6669C19.2857 11.893 19.2612 12.1226 19.2575 12.3531C19.2575 13.1904 19.2575 14.0299 19.2575 14.8695C19.2575 14.8946 19.2575 14.9198 19.2575 14.9564H23.0229C23.1807 14.9564 23.183 14.9564 23.1624 15.1074C23.0778 15.7662 22.9885 16.425 22.9039 17.0816C22.8322 17.6321 22.7636 18.1827 22.698 18.7332C22.6729 18.9437 22.6797 18.9437 22.4693 18.9437H19.2644V28.8992C19.2644 28.9793 19.2644 29.0593 19.2644 29.1394L15.1147 29.1371Z" fill="white"></path>
<path id="Vector_2_Facebook" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
</g>
<defs>
<clipPath id="clip0_1717_1086">
<rect width="35" height="35" fill="white"></rect>
</clipPath>
</defs>
</svg>
</a></li>
<li class="list-inline-item"><a href="https://www.youtube.com/user/NLMNIH" aria-label="Youtube" target="_blank" rel="noopener noreferrer">
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
<title>Youtube</title>
<g id="YouTube" clip-path="url(#clip0_1717_1101)">
<path id="Vector_Youtube" d="M26.2571 11.4791C25.9025 11.1589 25.5709 10.9576 24.228 10.834C22.5512 10.6785 20.2797 10.6556 18.564 10.6533H16.4365C14.7208 10.6533 12.4493 10.6785 10.7725 10.834C9.43196 10.9576 9.09798 11.1589 8.7434 11.4791C7.81464 12.321 7.6202 14.6268 7.59961 16.8938C7.59961 17.3178 7.59961 17.741 7.59961 18.1635C7.62706 20.4121 7.82837 22.686 8.7434 23.521C9.09798 23.8412 9.42967 24.0425 10.7725 24.1661C12.4493 24.3216 14.7208 24.3445 16.4365 24.3468H18.564C20.2797 24.3468 22.5512 24.3216 24.228 24.1661C25.5686 24.0425 25.9025 23.8412 26.2571 23.521C27.1722 22.6929 27.3735 20.451 27.4009 18.2206C27.4009 17.7402 27.4009 17.2599 27.4009 16.7795C27.3735 14.5491 27.1699 12.3072 26.2571 11.4791ZM15.5604 20.5311V14.652L20.561 17.5001L15.5604 20.5311Z" fill="white"></path>
<path id="Vector_2_Youtube" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
</g>
<defs>
<clipPath id="clip0_1717_1101">
<rect width="35" height="35" fill="white"></rect>
</clipPath>
</defs>
</svg>
</a></li>
</ul>
</div>
<div class="col-lg-3 col-12">
<p class="address_footer text-white">National Library of Medicine<br />
<a href="https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/@38.9959508,-77.101021,17z/data=!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb:0x19156f88b27635b8!8m2!3d38.9959508!4d-77.0988323" class="text-white" target="_blank" rel="noopener noreferrer">8600 Rockville Pike<br />
Bethesda, MD 20894</a></p>
</div>
<div class="col-lg-3 col-12 centered-lg">
<p><a href="https://www.nlm.nih.gov/web_policies.html" class="text-white">Web Policies</a><br />
<a href="https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office" class="text-white">FOIA</a><br />
<a href="https://www.hhs.gov/vulnerability-disclosure-policy/index.html" class="text-white" id="vdp">HHS Vulnerability Disclosure</a></p>
</div>
<div class="col-lg-3 col-12 centered-lg">
<p><a class="supportLink text-white" href="https://support.nlm.nih.gov/">Help</a><br />
<a href="https://www.nlm.nih.gov/accessibility.html" class="text-white">Accessibility</a><br />
<a href="https://www.nlm.nih.gov/careers/careers.html" class="text-white">Careers</a></p>
</div>
</div>
<div class="row">
<div class="col-lg-12 centered-lg">
<nav class="bottom-links">
<ul class="mt-3">
<li>
<a class="text-white" href="//www.nlm.nih.gov/">NLM</a>
</li>
<li>
<a class="text-white" href="https://www.nih.gov/">NIH</a>
</li>
<li>
<a class="text-white" href="https://www.hhs.gov/">HHS</a>
</li>
<li>
<a class="text-white" href="https://www.usa.gov/">USA.gov</a>
</li>
</ul>
</nav>
</div>
</div>
</div>
</section>
<script type="text/javascript" src="/portal/portal3rc.fcgi/rlib/js/InstrumentOmnitureBaseJS/InstrumentNCBIConfigJS/InstrumentNCBIBaseJS/InstrumentPageStarterJS.js?v=1"> </script>
<script type="text/javascript" src="/portal/portal3rc.fcgi/static/js/hfjs2.js"> </script>
</div>
</div>
<!--/.footer-->
<p class="last-updated small">Last updated: 2024-04-26T20:26:57Z</p>
</div>
<!--/.page-->
</div>
<!--/.wrap-->
<span class="PAFAppResources"></span>
</div><!-- /.twelve_col -->
</div>
<!-- /.grid -->
<!-- usually for JS scripts at page bottom -->
<span class="pagefixtures"></span>
<!-- CE8B5AF87C7FFCB1_0191SID /projects/staticsites/genbank/genbank@2.21 portal107 v4.1.r689238 Tue, Oct 22 2024 16:10:51 -->
<span id="portal-csrf-token" style="display:none" data-token="CE8B5AF87C7FFCB1_0191SID"></span>
<script type="text/javascript" src="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/js/3879255/4121861/1490097/4087685.js" snapshot="genbank"></script></body>
</html>
Reference in a new issue View git blame Copy permalink