685 lines
41 KiB
XML
685 lines
41 KiB
XML
<?xml version="1.0" encoding="utf-8"?>
|
||
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
|
||
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
|
||
|
||
<head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
|
||
<!-- AppResources meta begin -->
|
||
<meta name="paf-app-resources" content="" />
|
||
<!-- AppResources meta end -->
|
||
|
||
<!-- TemplateResources meta begin -->
|
||
<meta name="paf_template" content="StdNCol" />
|
||
|
||
<!-- TemplateResources meta end -->
|
||
|
||
<!-- Page meta begin -->
|
||
|
||
<!-- Page meta end -->
|
||
|
||
<!-- Logger begin -->
|
||
<meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_app" content="genbank" /><meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="ncbi_pdid" content="custom-page" />
|
||
<!-- Logger end -->
|
||
|
||
<title>TSA Submission Guide</title>
|
||
|
||
<!-- PageFixtures headcontent begin -->
|
||
|
||
<meta name="cms-local-nav-url" content="https://cms.ncbi.nlm.nih.gov//genbank/_nav" />
|
||
|
||
<!-- PageFixtures headcontent end -->
|
||
|
||
<!-- AppResources external_resources begin -->
|
||
<script type="text/javascript" src="/core/jig/1.15.6/js/jig.min.js"></script>
|
||
|
||
<!-- AppResources external_resources end -->
|
||
|
||
<!-- Page headcontent begin -->
|
||
<meta name="subsite" content="genbank" />
|
||
<meta name="path" content="genbank/tsaguide" />
|
||
<meta name="modified" content="2024-10-09T12:38:06Z" /><meta xmlns:ncbi-portal="http://ncbi.gov/portal/XSLT/namespace" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" name="cms-edit-aux-url" content="http://cms.ncbi.nlm.nih.gov/node//edit" />
|
||
<!-- Page headcontent end -->
|
||
<!-- PageFixtures resources begin -->
|
||
<link xmlns="http://www.w3.org/1999/xhtml" type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218191/css/4207974/4206132.css" xml:base="http://127.0.0.1/sites/static/header_footer" />
|
||
|
||
<!-- PageFixtures resources end -->
|
||
<link rel="shortcut icon" href="//www.ncbi.nlm.nih.gov/favicon.ico" /><meta name="ncbi_phid" content="CE8B361D7C7F12210000000000FF00CE.m_6" />
|
||
<meta name='referrer' content='origin-when-cross-origin'/><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/css/4121862/3974050/3917732/251717/4108189/14534/45193/3534283/4128070/3407145/4005757/4062871.css" /><link type="text/css" rel="stylesheet" href="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/css/3529741/3529739.css" media="print" /></head>
|
||
<body class=" col2 custom-page">
|
||
<div class="grid">
|
||
<div class="col twelve_col nomargin shadow">
|
||
<!-- System messages like service outage or JS required; this is handled by the TemplateResources portlet -->
|
||
<div class="sysmessages">
|
||
<noscript>
|
||
<p class="nojs">
|
||
<strong>Warning:</strong>
|
||
The NCBI web site requires JavaScript to function.
|
||
<a href="/guide/browsers/#enablejs" title="Learn how to enable JavaScript" target="_blank">more...</a>
|
||
</p>
|
||
</noscript>
|
||
</div>
|
||
<!--/.sysmessage-->
|
||
<div class="wrap">
|
||
<div class="page">
|
||
<div xmlns:xi="http://www.w3.org/2001/XInclude">
|
||
<div xmlns="http://www.w3.org/1999/xhtml" id="universal_header" xml:base="http://127.0.0.1/sites/static/header_footer">
|
||
<section class="usa-banner">
|
||
<div class="usa-accordion">
|
||
<header class="usa-banner-header">
|
||
<div class="usa-grid usa-banner-inner">
|
||
<img src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/favicons/favicon-57.png" alt="U.S. flag" />
|
||
<p>An official website of the United States government</p>
|
||
<button class="non-usa-accordion-button usa-banner-button" aria-expanded="false" aria-controls="gov-banner-top" type="button">
|
||
<span class="usa-banner-button-text">Here's how you know</span>
|
||
</button>
|
||
</div>
|
||
</header>
|
||
<div class="usa-banner-content usa-grid usa-accordion-content" id="gov-banner-top" aria-hidden="true">
|
||
<div class="usa-banner-guidance-gov usa-width-one-half">
|
||
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-dot-gov.svg" alt="Dot gov" />
|
||
<div class="usa-media_block-body">
|
||
<p>
|
||
<strong>The .gov means it's official.</strong>
|
||
<br />
|
||
Federal government websites often end in .gov or .mil. Before
|
||
sharing sensitive information, make sure you're on a federal
|
||
government site.
|
||
</p>
|
||
</div>
|
||
</div>
|
||
<div class="usa-banner-guidance-ssl usa-width-one-half">
|
||
<img class="usa-banner-icon usa-media_block-img" src="https://www.ncbi.nlm.nih.gov/coreutils/uswds/img/icon-https.svg" alt="Https" />
|
||
<div class="usa-media_block-body">
|
||
<p>
|
||
<strong>The site is secure.</strong>
|
||
<br />
|
||
The <strong>https://</strong> ensures that you are connecting to the
|
||
official website and that any information you provide is encrypted
|
||
and transmitted securely.
|
||
</p>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</section>
|
||
<div class="usa-overlay"></div>
|
||
<header class="ncbi-header" role="banner" data-section="Header">
|
||
|
||
<div class="usa-grid">
|
||
<div class="usa-width-one-whole">
|
||
|
||
<div class="ncbi-header__logo">
|
||
<a href="/" class="logo" aria-label="NCBI Logo" data-ga-action="click_image" data-ga-label="NIH NLM Logo">
|
||
<img src="https://www.ncbi.nlm.nih.gov/coreutils/nwds/img/logos/AgencyLogo.svg" alt="NIH NLM Logo" />
|
||
</a>
|
||
</div>
|
||
|
||
<div class="ncbi-header__account">
|
||
<a id="account_login" href="https://account.ncbi.nlm.nih.gov" class="usa-button header-button" style="display:none" data-ga-action="open_menu" data-ga-label="account_menu">Log in</a>
|
||
<button id="account_info" class="header-button" style="display:none" aria-controls="account_popup" type="button">
|
||
<span class="fa fa-user" aria-hidden="true">
|
||
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 24 24" width="20px" height="20px">
|
||
<g style="fill: #fff">
|
||
<ellipse cx="12" cy="8" rx="5" ry="6"></ellipse>
|
||
<path d="M21.8,19.1c-0.9-1.8-2.6-3.3-4.8-4.2c-0.6-0.2-1.3-0.2-1.8,0.1c-1,0.6-2,0.9-3.2,0.9s-2.2-0.3-3.2-0.9 C8.3,14.8,7.6,14.7,7,15c-2.2,0.9-3.9,2.4-4.8,4.2C1.5,20.5,2.6,22,4.1,22h15.8C21.4,22,22.5,20.5,21.8,19.1z"></path>
|
||
</g>
|
||
</svg>
|
||
</span>
|
||
<span class="username desktop-only" aria-hidden="true" id="uname_short"></span>
|
||
<span class="sr-only">Show account info</span>
|
||
</button>
|
||
</div>
|
||
|
||
<div class="ncbi-popup-anchor">
|
||
<div class="ncbi-popup account-popup" id="account_popup" aria-hidden="true">
|
||
<div class="ncbi-popup-head">
|
||
<button class="ncbi-close-button" data-ga-action="close_menu" data-ga-label="account_menu" type="button">
|
||
<span class="fa fa-times">
|
||
<svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 48 48" width="24px" height="24px">
|
||
<path d="M38 12.83l-2.83-2.83-11.17 11.17-11.17-11.17-2.83 2.83 11.17 11.17-11.17 11.17 2.83 2.83 11.17-11.17 11.17 11.17 2.83-2.83-11.17-11.17z"></path>
|
||
</svg>
|
||
</span>
|
||
<span class="usa-sr-only">Close</span></button>
|
||
<h4>Account</h4>
|
||
</div>
|
||
<div class="account-user-info">
|
||
Logged in as:<br />
|
||
<b><span class="username" id="uname_long">username</span></b>
|
||
</div>
|
||
<div class="account-links">
|
||
<ul class="usa-unstyled-list">
|
||
<li><a id="account_myncbi" href="/myncbi/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_myncbi">Dashboard</a></li>
|
||
<li><a id="account_pubs" href="/myncbi/collections/bibliography/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_pubs">Publications</a></li>
|
||
<li><a id="account_settings" href="/account/settings/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_settings">Account settings</a></li>
|
||
<li><a id="account_logout" href="/account/signout/" class="set-base-url" data-ga-action="click_menu_item" data-ga-label="account_logout">Log out</a></li>
|
||
</ul>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
|
||
</div>
|
||
</div>
|
||
</header>
|
||
<div role="navigation" aria-label="access keys">
|
||
<a id="nws_header_accesskey_0" href="https://www.ncbi.nlm.nih.gov/guide/browsers/#ncbi_accesskeys" class="usa-sr-only" accesskey="0" tabindex="-1">Access keys</a>
|
||
<a id="nws_header_accesskey_1" href="https://www.ncbi.nlm.nih.gov" class="usa-sr-only" accesskey="1" tabindex="-1">NCBI Homepage</a>
|
||
<a id="nws_header_accesskey_2" href="/myncbi/" class="set-base-url usa-sr-only" accesskey="2" tabindex="-1">MyNCBI Homepage</a>
|
||
<a id="nws_header_accesskey_3" href="#maincontent" class="usa-sr-only" accesskey="3" tabindex="-1">Main Content</a>
|
||
<a id="nws_header_accesskey_4" href="#" class="usa-sr-only" accesskey="4" tabindex="-1">Main Navigation</a>
|
||
</div>
|
||
<section data-section="Alerts">
|
||
<div class="ncbi-alerts-placeholder"></div>
|
||
</section>
|
||
</div>
|
||
</div>
|
||
<!--/.header-->
|
||
<div class="header">
|
||
<div class="res_logo"><h1 class="res_name"><a href="/genbank/" title="GenBank home">GenBank</a></h1><h2 class="res_tagline">Public nucleic acid sequence repository</h2></div>
|
||
<div class="search"><form method="get" action="/nuccore/"><div class="search_form"><label for="database" class="offscreen_noflow">Search database</label><select id="database"><optgroup label="Recent"><option value="nuccore" selected="selected">Nucleotide</option><option value="sra">SRA</option><option value="books">Books</option><option value="clinvar" class="last">ClinVar</option></optgroup><optgroup label="All"><option value="gquery">All Databases</option><option value="assembly">Assembly</option><option value="biocollections">Biocollections</option><option value="bioproject">BioProject</option><option value="biosample">BioSample</option><option value="books">Books</option><option value="clinvar">ClinVar</option><option value="cdd">Conserved Domains</option><option value="gap">dbGaP</option><option value="dbvar">dbVar</option><option value="gene">Gene</option><option value="genome">Genome</option><option value="gds">GEO DataSets</option><option value="geoprofiles">GEO Profiles</option><option value="gtr">GTR</option><option value="ipg">Identical Protein Groups</option><option value="medgen">MedGen</option><option value="mesh">MeSH</option><option value="nlmcatalog">NLM Catalog</option><option value="nuccore">Nucleotide</option><option value="omim">OMIM</option><option value="pmc">PMC</option><option value="protein">Protein</option><option value="proteinclusters">Protein Clusters</option><option value="protfam">Protein Family Models</option><option value="pcassay">PubChem BioAssay</option><option value="pccompound">PubChem Compound</option><option value="pcsubstance">PubChem Substance</option><option value="pubmed">PubMed</option><option value="snp">SNP</option><option value="sra">SRA</option><option value="structure">Structure</option><option value="taxonomy">Taxonomy</option><option value="toolkit">ToolKit</option><option value="toolkitall">ToolKitAll</option><option value="toolkitbookgh">ToolKitBookgh</option></optgroup></select><div class="nowrap"><label for="term" class="offscreen_noflow" accesskey="/">Search term</label><div class="nowrap"><input type="text" name="term" id="term" title="Search Nucleotide" value="" class="jig-ncbiclearbutton jig-ncbiautocomplete" data-jigconfig="isEnabled:false,disableUrl:'NcbiSearchBarAutoComplCtrl'" autocomplete="off" data-sbconfig="ds:'no',pjs:'no',afs:'yes'" /></div><button id="search" type="submit" class="button_search nowrap" cmd="go">Search</button></div></div></form></div>
|
||
|
||
</div>
|
||
<div class="nav_and_browser">
|
||
<div class="localnav"><ul class="jig-ncbilocalnav">
|
||
<li><a href="#">GenBank</a><ul>
|
||
<li><a href="/genbank/">About GenBank</a></li>
|
||
<li><a href="/genbank/submit_types">Submission Types</a></li>
|
||
<li><a href="/genbank/submit">Submission Tools</a></li>
|
||
<li><a href="/genbank/update">Update GenBank Records</a></li>
|
||
<li><a href="/nuccore/">Search</a></li>
|
||
<li><a href="/BLAST/Blast.cgi?CMD=Web&PAGETYPE=BLASTHome">BLAST</a></li>
|
||
<li><a href="/genbank/statistics">Statistics</a></li>
|
||
<li><a href="/genbank/samplerecord/">Sample Record</a></li>
|
||
<li><a href="/genbank/sequencerevisionhistory/">Revision History</a></li>
|
||
<li><a href="/genbank/sequenceids/">Sequence IDs</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Submit</a><ul>
|
||
<li><a href="/genbank/submit">Submission Tools</a></li>
|
||
<li><a href="/genbank/submit_types">Submission Types</a></li>
|
||
<li><a href="/WebSub/?tool=genbank">BankIt</a></li>
|
||
<li><a href="/genbank/table2asn">table2asn</a></li>
|
||
<li><a href="https://www.ncbi.nlm.nih.gov/sra/docs/sequence-data-processing">Sequence Data Processing</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Genomes</a><ul>
|
||
<li><a href="/genbank/genomesubmit">Complete Genome Submission Guide</a></li>
|
||
<li><a href="/genbank/genomesubmit_annotation">Prokaryotic Genome Annotation Guide</a></li>
|
||
<li><a href="/genbank/eukaryotic_genome_submission_annotation">Eukaryotic Genome Annotation Guide</a></li>
|
||
<li><a href="/genbank/examples.wgs">Annotation Examples</a></li>
|
||
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/wgs/">Genome Submission Portal</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a title="Whole Genome Shotgun sequences and submissions" href="#">WGS</a><ul>
|
||
<li><a href="/genbank/wgs">About WGS</a></li>
|
||
<li><a href="/Traces/wgs">WGS Project List</a></li>
|
||
<li><a href="/genbank/wgs.submit">WGS Submission Guide</a></li>
|
||
<li><a href="/genbank/wgsfaq/">FAQ</a></li>
|
||
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/wgs/">Genome Submission Portal</a></li>
|
||
<li><a href="/genbank/eukaryotic_genome_submission_annotation">Eukaryotic Annotation Guide</a></li>
|
||
<li><a href="/genbank/genomesubmit_annotation">Prokaryotic Annotation Guide</a></li>
|
||
<li><a href="/genbank/asndisc">Discrepancy Report</a></li>
|
||
<li><a href="/assembly/agp/AGP_Specification/">AGP format</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Metagenomes</a><ul>
|
||
<li><a href="/genbank/metagenome">About Metagenomes</a></li>
|
||
<li><a href="/genbank/structuredcomment">Structured Comment</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">TPA</a><ul>
|
||
<li><a href="/genbank/TPA">About TPA</a></li>
|
||
<li><a href="/genbank/tpafaq">FAQ</a></li>
|
||
<li><a href="/genbank/TPA-Exp">TPA-Exp</a></li>
|
||
<li><a href="/genbank/TPA-Inf">TPA-Inf</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">TSA</a><ul>
|
||
<li><a href="/genbank/TSA">About TSA</a></li>
|
||
<li><a href="/genbank/TSAguide">TSA Submission Guide</a></li>
|
||
<li><a href="/genbank/TSAfaq">FAQ</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">INSDC</a><ul>
|
||
<li><a href="/genbank/collab">About INSDC</a></li>
|
||
<li><a href="/genbank/collab/country">Geographic Location Name List</a></li>
|
||
<li><a href="/genbank/collab/db_xref">db_xref List</a></li>
|
||
<li><a href="http://www.insdc.org/documents/feature_table.html">Feature Table</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Documentation</a><ul>
|
||
<li><a href="https://www.ncbi.nlm.nih.gov/sra/docs/sequence-data-processing/">Sequence Data Processing</a></li>
|
||
<li><a href="/genbank/submission_brokers">Submission Brokers</a></li>
|
||
<li><a href="/genbank/acc_prefix">Accession Number Prefixes</a></li>
|
||
<li><a href="/genbank/organelle_submit/">Organelle Submission Guide</a></li>
|
||
<li><a href="/genbank/monkeypox_submission/">Monkeypox Submission Guide</a></li>
|
||
<li><a href="/genbank/validation/">Common Submission Errors</a> </li>
|
||
<li><a href="/genbank/sequencecheck/">Ribosomal Submission Errors</a></li>
|
||
<li><a href="/genbank/sequencecheck/virus">Common Sequence Errors</a></li>
|
||
<li><a href="https://support.nlm.nih.gov/knowledgebase/category/?id=CAT-01240">Submission FAQs</a></li>
|
||
</ul>
|
||
</li>
|
||
<li><a href="#">Other</a><ul>
|
||
<li><a href="/genbank/htgs">About HTGs</a></li>
|
||
<li><a href="/genbank/dbest">About EST</a></li>
|
||
<li><a href="/genbank/dbgss">About GSS</a></li>
|
||
<li><a href="/genbank/tls">About TLS</a></li>
|
||
<li><a href="/genbank/tlsguide">Submit TLS</a></li>
|
||
</ul>
|
||
</li>
|
||
</ul></div>
|
||
</div>
|
||
|
||
<!-- was itemctrl -->
|
||
<div class="container">
|
||
<div id="maincontent" class="content col twelve_col last">
|
||
<div class="col1">
|
||
<h1 id="tsa-submission-guide">TSA Submission Guide</h1>
|
||
|
||
|
||
<h2 id="RQ">Requirements</h2>
|
||
|
||
|
||
<ul>
|
||
<li>Raw reads should be submitted to <a href="https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi">SRA</a> prior to submitting your transcriptome. The SRA run accession(s) (SRRXXXXXX) and associated BioProject (PRJNAXXXXXX) and BioSample(s) (SAMNXXXXXX) are required for TSA submission.</li>
|
||
<li>Assembly Data structured comment. This information is input directly in the Submission Portal dialogs.</li>
|
||
<li>Description of the assembly process if a multi-step assembly was performed should be provided in the COMMENT section.</li>
|
||
<li>If annotation is provided the product names should follow the <a href="https://www.ncbi.nlm.nih.gov/genome/doc/internatprot_nomenguide/">International Protein Nomenclature Guidelines</a>.</li>
|
||
<li>Annotation must be biologically valid.</li>
|
||
<li>The keyword 'Targeted' and feature annotation should be included for all targeted subsets of transcriptome data. See <a href="#target">Targeted vs. Non-targeted TSA Studies</a> for more information.</li>
|
||
</ul>
|
||
|
||
|
||
<h2 id="creating-the-tsa-submission-file">Creating the TSA submission file</h2>
|
||
|
||
|
||
<p>[1] The BioProject accession, BioSample accession(s), SRA run accession(s) and Assembly Structured Comment data are entered using the Submission Portal dialogs. </p>
|
||
|
||
|
||
<p>[2] If submitting a Targeted subset of your data see the additional requirements under <a href="#target">Targeted vs. Non-targeted TSA</a> .</p>
|
||
|
||
|
||
<p>[3] All TSA submissions are submitted through the TSA <a href="#SP">Submission Portal</a> .</p>
|
||
|
||
|
||
<p>[4] Submit either a fasta or .sqn file</p>
|
||
|
||
|
||
<ul>
|
||
<li>
|
||
<p>Preparing a fasta file for submission:</p>
|
||
<ul>
|
||
<li>Sequences should be in <a href="/genbank/tbl2asn2#fsa">fasta format.</a></li>
|
||
<li>Files have the suffix .fsa.</li>
|
||
<li>fasta defline component: [moltype=transcribed_RNA]</li>
|
||
<li>Each sequence has a definition line beginning with a unique identifier, eg contig001, contig002, etc.<ul>
|
||
<li>Use concise names for the unique indentifier that do not include length or coverage information.</li>
|
||
<li>The unique identifier cannot exceed 50 characters. </li>
|
||
<li>If uploading multiple fasta file sets in one submission (SUB) the sequence identifier must be unique across all sets.</li>
|
||
<li>The unique identifier appears in the DEFINITION line in the flatfile view of the TSA flatfiles.</li>
|
||
</ul>
|
||
</li>
|
||
<li>Contigs should >199 nt.</li>
|
||
<li>Remove any n's from the beginning or end of each sequence.</li>
|
||
<li>Do not include internal n's that represent a gap of unknown length. These sequences should be split at the gap.</li>
|
||
<li>Any n stretches greater than 14 nucleotides will need an assembly gap feature. The Submission Portal will will provide a prompt to set up the assembly gap feature.</li>
|
||
<li>Scan the sequences using the NCBI Foreign Contamination Screen <a href="https://github.com/ncbi/fcs-gx">FCS-GX</a> to avoid delays after submission. </li>
|
||
</ul>
|
||
</li>
|
||
<li>
|
||
<p>Preparing .asn file using <a href="/genbank/table2asn">table2asn</a> for submission.</p>
|
||
<ul>
|
||
<li><a href="/genbank/table2asn">table2asn</a> reads a <a href="//www.ncbi.nlm.nih.gov/WebSub/template.cgi">template.sbt</a> along with the sequence and table files, and outputs ASN.1 for submission to TSA through the portal.</li>
|
||
<li>Annotation may be included using a Feature table. See <a href="/genbank/table2asn">table2asn</a> .</li>
|
||
<li>
|
||
<p>fasta defline components:</p>
|
||
<ul>
|
||
<li>[moltype=transcribed_RNA]</li>
|
||
<li>[tech=TSA]</li>
|
||
<li>To add Source information see <a href="/genbank/table2asn">table2asn</a> Source table format</li>
|
||
</ul>
|
||
<p>Sample command line:</p>
|
||
<pre><code> table2asn -t template.sbt -indir . -Y comment -M t
|
||
</code></pre>
|
||
<p>The validator output (*.val) should be reviewed before submitting. Any validator errors not resolved prior to submission may be stopped in the Submission Portal. See <a href="#SP">Submitting the file to TSA-Submission Portal</a> for more information.</p>
|
||
<p><table border="1" cellpadding="0" cellspacing="0">
|
||
<caption>tbl2asn command line arguments</caption>
|
||
<tbody>
|
||
<tr>
|
||
<td>-Y</td>
|
||
<td>To import Assembly Description Comment</td>
|
||
</tr>
|
||
<tr>
|
||
<td>-M t</td>
|
||
<td>To run standard validator and additional TSA checks</td>
|
||
</tr>
|
||
<tr>
|
||
<td>-j</td>
|
||
<td>Allows the addition of <a href="//www.ncbi.nlm.nih.gov/Sequin/modifiers.html">source qualifiers</a> that will be the same for each submission.<p>Example: -j "[organism=Homo sapiens] [tissue-type=liver]"</p></td>
|
||
</tr>
|
||
</tbody>
|
||
</table>
|
||
</p>
|
||
</li>
|
||
</ul>
|
||
</li>
|
||
</ul>
|
||
|
||
|
||
<h2 id="SP">Submitting the file to TSA Submission Portal</h2>
|
||
|
||
|
||
<p>All files must be submitted via the <a href="https://submit.ncbi.nlm.nih.gov/subs/tsa/">Submission Portal</a> .</p>
|
||
|
||
|
||
<p>When the file is uploaded it will undergo a series of validation checks. The following will stop your submission in portal:</p>
|
||
|
||
|
||
<ul>
|
||
<li>Sequences less than 200 bp</li>
|
||
<li>Sequences with univec hits that are for Next-Gen sequencing primers</li>
|
||
<li>Sequences that are more than 10% n's or have more than 14n's in a row</li>
|
||
<li>Files that are incorrectly formatted or have biologically invalid annotation</li>
|
||
</ul>
|
||
|
||
|
||
<p>Submission statuses in the Submission Portal:</p>
|
||
|
||
|
||
<ul>
|
||
<li>Queued: The submission is successful and waiting for review by TSA staff. If there are any issues the submitter will be contacted with a list of revisions and/or inquiries.</li>
|
||
<li>Error: The TSA staff has reported any error(s) to the submitter. The corrections need to be made and a new file uploaded using the Fix button.</li>
|
||
<li>Processing: The submission has been successfully completed and an accession number for the project has been assigned.</li>
|
||
<li>Processed: The project has been released to the database.</li>
|
||
</ul>
|
||
|
||
|
||
<h2 id="meta">Metagenomic Transcription Studies</h2>
|
||
|
||
|
||
<p>For metagenomic transcriptome studies the regular submission process should be followed with the following adjustments:</p>
|
||
|
||
|
||
<ul>
|
||
<li>Use a metagenome organism name that describes the sample from which the DNA was isolated (eg soil metagenome or gut metagenome). The NCBI Taxonomy database lists several metagenomic names. The name is selected in the first steps when submitting to SRA or registering your BioSamples.</li>
|
||
<li>To save processing time please remove potential contaminant sequences prior to submitting. Scan the sequences using the NCBI Foreign Contamination Screen <a href="https://github.com/ncbi/fcs-gx">FCS-GX</a></li>
|
||
<li>Since TSA runs several validation checks on each transcriptome assembly, you may need to split large assembly files into smaller files.</li>
|
||
</ul>
|
||
|
||
|
||
<h2 id="target">Targeted vs. Non-targeted TSA Studies</h2>
|
||
|
||
|
||
<p>It is expected that submissions to TSA would comprise a large-scale comprehensive study of the complete transcriptome of an organism. However, some scientists do targeted studies of their transcriptome data and only want to submit this small subset. For targeted studies the regular submission process should be followed with the following requirements:</p>
|
||
|
||
|
||
<ul>
|
||
<li>The keyword 'Targeted' should be added to the submission file. Using tbl2asn this can be done by including [keyword=Targeted] in the fasta definition line.</li>
|
||
<li>Annotation must be included showing the focus of the targeted study. This can be done with a gene, misc_feature, or RNA feature.</li>
|
||
<li>If coding regions are provided the product names should follow the <a href="https://www.ncbi.nlm.nih.gov/genome/doc/internatprot_nomenguide/">International Protein Nomenclature Guidelines</a>. If misc_features are provided then the /note should be in the following format "similar to product_name".</li>
|
||
<li>Set the molecule type (moltype) to the appropriate RNA type -mRNA, rRNA, ncRNA, or transcribed RNA.</li>
|
||
</ul>
|
||
|
||
|
||
<p>*SRA cannot release a subportion of your data to match your subset. The entire SRA dataset will be released upon release of your subset.</p>
|
||
|
||
|
||
<h2 id="assembly-gaps">Assembly Gaps</h2>
|
||
|
||
|
||
<p>Sequences with known gaps can be submitted to TSA providing the gap is annotated with an assembly_gap feature.</p>
|
||
|
||
|
||
<p>The required qualifiers for the assembly_gap feature are:</p>
|
||
|
||
|
||
<ul>
|
||
<li>estimated_length</li>
|
||
<li>linkage_evidence<ul>
|
||
<li>paired-ends: paired sequences from the two ends of a DNA fragment.</li>
|
||
<li>align-genus: alignment to a reference genome within the same genus.</li>
|
||
<li>align-xgenus: alignment to a reference genome within another genus.</li>
|
||
<li>align-trnscpt: alignment to a transcript from the same species.</li>
|
||
</ul>
|
||
</li>
|
||
</ul>
|
||
|
||
|
||
<h2 id="updating">Updating TSA submissions</h2>
|
||
|
||
|
||
<p>See <a href="/genbank/tsaupdate">Update TSA Records</a> for instructions on how to update your assemblies. Contact gb-admin@ncbi.nlm.nih.gov with any additional questions.</p>
|
||
|
||
|
||
</div>
|
||
<!--/.col1-->
|
||
<div class="col2">
|
||
<div class="rightnav">
|
||
<h2 id="tsa-resources">TSA Resources</h2>
|
||
<ul>
|
||
<li><a href="/genbank/TSA">About TSA</a></li>
|
||
<li><a href="/genbank/TSAguide">TSA Submission Guide</a></li>
|
||
<li><a href="/genbank/TSAfaq">FAQ</a></li>
|
||
<li><a href="/genbank/tsaupdate">Update TSA Records</a></li>
|
||
<li><a href="/genbank/table2asn">table2asn</a></li>
|
||
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/">Submission Portal</a></li>
|
||
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/bioproject/">BioProject</a></li>
|
||
<li><a href="https://submit.ncbi.nlm.nih.gov/subs/biosample/">BioSample</a></li>
|
||
<li><a href="https://www.ncbi.nlm.nih.gov/Traces/wgs/?view=TSA">TSA Browser</a></li>
|
||
<li><a href="https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi">SRA</a></li>
|
||
</ul>
|
||
</div>
|
||
</div>
|
||
<!--/.col2-->
|
||
<div class="col3">
|
||
|
||
</div>
|
||
<!--/.col3-->
|
||
<div class="col4">
|
||
|
||
</div>
|
||
<!--/.col4-->
|
||
<div class="col5">
|
||
|
||
</div>
|
||
<div class="col6">
|
||
|
||
</div>
|
||
<div class="col7">
|
||
|
||
</div>
|
||
<div class="col8">
|
||
|
||
</div>
|
||
<div class="col9">
|
||
|
||
</div>
|
||
</div><!--/.content-->
|
||
</div><!--/.container-->
|
||
<div id="NCBIFooter_dynamic">
|
||
<div class="breadcrumbs">You are here:
|
||
<span id="breadcrumb_text"><a href="/guide/">NCBI</a></span></div>
|
||
<a id="help-desk-link" class="help_desk" href="https://support.ncbi.nlm.nih.gov/ics/support/default.asp?Time=2025-03-05T02:45:47-05:00&Snapshot=%2Fprojects%2Fstaticsites%2Fgenbank%2Fgenbank@2.21&Host=portal104&ncbi_phid=CE8B361D7C7F12210000000000FF00CE&ncbi_session=CE8B5AF87C7FFCB1_0191SID&from=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fgenbank%2Ftsaguide%2F&Ncbi_App=genbank&Page=custom-page&style=classic&deptID=28049" target="_blank">Support Center</a>
|
||
<noscript><img alt="" src="/stat?jsdisabled=true&ncbi_app=genbank&ncbi_db=&ncbi_pdid=custom-page&ncbi_phid=CE8B361D7C7F12210000000000FF00CE" /></noscript>
|
||
</div>
|
||
|
||
|
||
<div xmlns:xi="http://www.w3.org/2001/XInclude">
|
||
<div xmlns="http://www.w3.org/1999/xhtml" class="footer" id="footer" xml:base="http://127.0.0.1/sites/static/header_footer">
|
||
<section class="icon-section">
|
||
<div id="icon-section-header" class="icon-section_header">Follow NCBI</div>
|
||
<div class="grid-container container">
|
||
<div class="icon-section_container">
|
||
<a class="footer-icon" id="footer_twitter" href="https://twitter.com/ncbi" aria-label="Twitter">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="40" height="40" viewBox="0 0 40 40" fill="none">
|
||
<title>Twitter</title>
|
||
<g id="twitterx1008">
|
||
<path id="path1008" d="M6.06736 7L16.8778 20.8991L6.00001 32.2H10.2L18.6 23.1L25.668 32.2H34L22.8 17.5L31.9 7H28.4L20.7 15.4L14.401 7H6.06898H6.06736ZM9.66753 8.73423H12.9327L29.7327 30.4658H26.5697L9.66753 8.73423Z" fill="#5B616B"></path>
|
||
</g>
|
||
</svg>
|
||
</a>
|
||
<a class="footer-icon" id="footer_facebook" href="https://www.facebook.com/ncbi.nlm" aria-label="Facebook"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
||
<title>Facebook</title>
|
||
<path class="cls-11" d="M210.5,115.12H171.74V97.82c0-8.14,5.39-10,9.19-10h27.14V52l-39.32-.12c-35.66,0-42.42,26.68-42.42,43.77v19.48H99.09v36.32h27.24v109h45.41v-109h35Z">
|
||
</path>
|
||
</svg></a>
|
||
<a class="footer-icon" id="footer_linkedin" href="https://www.linkedin.com/company/ncbinlm" aria-label="LinkedIn"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
||
<title>LinkedIn</title>
|
||
<path class="cls-11" d="M101.64,243.37H57.79v-114h43.85Zm-22-131.54h-.26c-13.25,0-21.82-10.36-21.82-21.76,0-11.65,8.84-21.15,22.33-21.15S101.7,78.72,102,90.38C102,101.77,93.4,111.83,79.63,111.83Zm100.93,52.61A17.54,17.54,0,0,0,163,182v61.39H119.18s.51-105.23,0-114H163v13a54.33,54.33,0,0,1,34.54-12.66c26,0,44.39,18.8,44.39,55.29v58.35H198.1V182A17.54,17.54,0,0,0,180.56,164.44Z">
|
||
</path>
|
||
</svg></a>
|
||
<a class="footer-icon" id="footer_github" href="https://github.com/ncbi" aria-label="GitHub"><svg xmlns="http://www.w3.org/2000/svg" data-name="Layer 1" viewBox="0 0 300 300">
|
||
<defs>
|
||
<style>
|
||
.cls-11,
|
||
.cls-12 {
|
||
fill: #737373;
|
||
}
|
||
|
||
.cls-11 {
|
||
fill-rule: evenodd;
|
||
}
|
||
</style>
|
||
</defs>
|
||
<title>GitHub</title>
|
||
<path class="cls-11" d="M151.36,47.28a105.76,105.76,0,0,0-33.43,206.1c5.28,1,7.22-2.3,7.22-5.09,0-2.52-.09-10.85-.14-19.69-29.42,6.4-35.63-12.48-35.63-12.48-4.81-12.22-11.74-15.47-11.74-15.47-9.59-6.56.73-6.43.73-6.43,10.61.75,16.21,10.9,16.21,10.9,9.43,16.17,24.73,11.49,30.77,8.79,1-6.83,3.69-11.5,6.71-14.14C108.57,197.1,83.88,188,83.88,147.51a40.92,40.92,0,0,1,10.9-28.39c-1.1-2.66-4.72-13.42,1-28,0,0,8.88-2.84,29.09,10.84a100.26,100.26,0,0,1,53,0C198,88.3,206.9,91.14,206.9,91.14c5.76,14.56,2.14,25.32,1,28a40.87,40.87,0,0,1,10.89,28.39c0,40.62-24.74,49.56-48.29,52.18,3.79,3.28,7.17,9.71,7.17,19.58,0,14.15-.12,25.54-.12,29,0,2.82,1.9,6.11,7.26,5.07A105.76,105.76,0,0,0,151.36,47.28Z">
|
||
</path>
|
||
<path class="cls-12" d="M85.66,199.12c-.23.52-1.06.68-1.81.32s-1.2-1.06-.95-1.59,1.06-.69,1.82-.33,1.21,1.07.94,1.6Zm-1.3-1">
|
||
</path>
|
||
<path class="cls-12" d="M90,203.89c-.51.47-1.49.25-2.16-.49a1.61,1.61,0,0,1-.31-2.19c.52-.47,1.47-.25,2.17.49s.82,1.72.3,2.19Zm-1-1.08">
|
||
</path>
|
||
<path class="cls-12" d="M94.12,210c-.65.46-1.71,0-2.37-.91s-.64-2.07,0-2.52,1.7,0,2.36.89.65,2.08,0,2.54Zm0,0"></path>
|
||
<path class="cls-12" d="M99.83,215.87c-.58.64-1.82.47-2.72-.41s-1.18-2.06-.6-2.7,1.83-.46,2.74.41,1.2,2.07.58,2.7Zm0,0">
|
||
</path>
|
||
<path class="cls-12" d="M107.71,219.29c-.26.82-1.45,1.2-2.64.85s-2-1.34-1.74-2.17,1.44-1.23,2.65-.85,2,1.32,1.73,2.17Zm0,0">
|
||
</path>
|
||
<path class="cls-12" d="M116.36,219.92c0,.87-1,1.59-2.24,1.61s-2.29-.68-2.3-1.54,1-1.59,2.26-1.61,2.28.67,2.28,1.54Zm0,0">
|
||
</path>
|
||
<path class="cls-12" d="M124.42,218.55c.15.85-.73,1.72-2,1.95s-2.37-.3-2.52-1.14.73-1.75,2-2,2.37.29,2.53,1.16Zm0,0"></path>
|
||
</svg></a>
|
||
<a class="footer-icon" id="footer_blog" href="https://ncbiinsights.ncbi.nlm.nih.gov/" aria-label="Blog">
|
||
<svg xmlns="http://www.w3.org/2000/svg" id="Layer_1" data-name="Layer 1" viewBox="0 0 40 40">
|
||
<defs><style>.cls-1{fill:#737373;}</style></defs>
|
||
<title>NCBI Insights Blog</title>
|
||
<path class="cls-1" d="M14,30a4,4,0,1,1-4-4,4,4,0,0,1,4,4Zm11,3A19,19,0,0,0,7.05,15a1,1,0,0,0-1,1v3a1,1,0,0,0,.93,1A14,14,0,0,1,20,33.07,1,1,0,0,0,21,34h3a1,1,0,0,0,1-1Zm9,0A28,28,0,0,0,7,6,1,1,0,0,0,6,7v3a1,1,0,0,0,1,1A23,23,0,0,1,29,33a1,1,0,0,0,1,1h3A1,1,0,0,0,34,33Z"></path>
|
||
</svg>
|
||
</a>
|
||
</div>
|
||
</div>
|
||
</section>
|
||
|
||
<section class="container-fluid bg-primary">
|
||
<div class="container pt-5">
|
||
<div class="row mt-3">
|
||
<div class="col-lg-3 col-12">
|
||
<p><a class="text-white" href="https://www.nlm.nih.gov/socialmedia/index.html">Connect with NLM</a></p>
|
||
<ul class="list-inline social_media">
|
||
<li class="list-inline-item"><a href="https://twitter.com/NLM_NIH" aria-label="Twitter" target="_blank" rel="noopener noreferrer">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
||
<title>Twitter</title>
|
||
<g id="twitterx1009" clip-path="url(#clip0_65276_3946)">
|
||
<path id="Vector_Twitter" d="M17.5006 34.6565C26.9761 34.6565 34.6575 26.9751 34.6575 17.4996C34.6575 8.02416 26.9761 0.342773 17.5006 0.342773C8.02514 0.342773 0.34375 8.02416 0.34375 17.4996C0.34375 26.9751 8.02514 34.6565 17.5006 34.6565Z" fill="#205493" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
||
<path id="path1009" d="M8.54811 8.5L16.2698 18.4279L8.50001 26.5H11.5L17.5 20L22.5486 26.5H28.5L20.5 16L27 8.5H24.5L19 14.5L14.5007 8.5H8.54927H8.54811ZM11.1197 9.73873H13.4519L25.4519 25.2613H23.1926L11.1197 9.73873Z" fill="white"></path>
|
||
</g>
|
||
<defs>
|
||
<clipPath id="clip0_65276_3946">
|
||
<rect width="35" height="35" fill="white"></rect>
|
||
</clipPath>
|
||
</defs>
|
||
</svg>
|
||
</a></li>
|
||
<li class="list-inline-item"><a href="https://www.facebook.com/nationallibraryofmedicine" aria-label="Facebook" rel="noopener noreferrer" target="_blank">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
||
<title>Facebook</title>
|
||
<g id="Facebook" clip-path="url(#clip0_1717_1086)">
|
||
<path id="Vector_Facebook" d="M15.1147 29.1371C15.1147 29.0822 15.1147 29.0296 15.1147 28.9747V18.9414H11.8183C11.6719 18.9414 11.6719 18.9414 11.6719 18.8018C11.6719 17.5642 11.6719 16.3289 11.6719 15.0937C11.6719 14.9793 11.7062 14.9518 11.816 14.9518C12.8683 14.9518 13.9206 14.9518 14.9751 14.9518H15.1215V14.8329C15.1215 13.8057 15.1215 12.774 15.1215 11.7492C15.1274 10.9262 15.3148 10.1146 15.6706 9.37241C16.1301 8.38271 16.9475 7.60378 17.9582 7.19235C18.6492 6.90525 19.3923 6.76428 20.1405 6.7783C21.0029 6.79202 21.8653 6.83091 22.7278 6.86065C22.8879 6.86065 23.048 6.89496 23.2082 6.90182C23.2974 6.90182 23.3271 6.94071 23.3271 7.02993C23.3271 7.54235 23.3271 8.05477 23.3271 8.5649C23.3271 9.16882 23.3271 9.77274 23.3271 10.3767C23.3271 10.4819 23.2974 10.5139 23.1921 10.5116C22.5379 10.5116 21.8814 10.5116 21.2271 10.5116C20.9287 10.5184 20.6316 10.5528 20.3395 10.6146C20.0822 10.6619 19.8463 10.7891 19.6653 10.9779C19.4842 11.1668 19.3672 11.4078 19.3307 11.6669C19.2857 11.893 19.2612 12.1226 19.2575 12.3531C19.2575 13.1904 19.2575 14.0299 19.2575 14.8695C19.2575 14.8946 19.2575 14.9198 19.2575 14.9564H23.0229C23.1807 14.9564 23.183 14.9564 23.1624 15.1074C23.0778 15.7662 22.9885 16.425 22.9039 17.0816C22.8322 17.6321 22.7636 18.1827 22.698 18.7332C22.6729 18.9437 22.6797 18.9437 22.4693 18.9437H19.2644V28.8992C19.2644 28.9793 19.2644 29.0593 19.2644 29.1394L15.1147 29.1371Z" fill="white"></path>
|
||
<path id="Vector_2_Facebook" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
||
</g>
|
||
<defs>
|
||
<clipPath id="clip0_1717_1086">
|
||
<rect width="35" height="35" fill="white"></rect>
|
||
</clipPath>
|
||
</defs>
|
||
</svg>
|
||
</a></li>
|
||
<li class="list-inline-item"><a href="https://www.youtube.com/user/NLMNIH" aria-label="Youtube" target="_blank" rel="noopener noreferrer">
|
||
<svg xmlns="http://www.w3.org/2000/svg" width="35" height="35" viewBox="0 0 36 35" fill="none">
|
||
<title>Youtube</title>
|
||
<g id="YouTube" clip-path="url(#clip0_1717_1101)">
|
||
<path id="Vector_Youtube" d="M26.2571 11.4791C25.9025 11.1589 25.5709 10.9576 24.228 10.834C22.5512 10.6785 20.2797 10.6556 18.564 10.6533H16.4365C14.7208 10.6533 12.4493 10.6785 10.7725 10.834C9.43196 10.9576 9.09798 11.1589 8.7434 11.4791C7.81464 12.321 7.6202 14.6268 7.59961 16.8938C7.59961 17.3178 7.59961 17.741 7.59961 18.1635C7.62706 20.4121 7.82837 22.686 8.7434 23.521C9.09798 23.8412 9.42967 24.0425 10.7725 24.1661C12.4493 24.3216 14.7208 24.3445 16.4365 24.3468H18.564C20.2797 24.3468 22.5512 24.3216 24.228 24.1661C25.5686 24.0425 25.9025 23.8412 26.2571 23.521C27.1722 22.6929 27.3735 20.451 27.4009 18.2206C27.4009 17.7402 27.4009 17.2599 27.4009 16.7795C27.3735 14.5491 27.1699 12.3072 26.2571 11.4791ZM15.5604 20.5311V14.652L20.561 17.5001L15.5604 20.5311Z" fill="white"></path>
|
||
<path id="Vector_2_Youtube" d="M17.5006 34.657C26.9761 34.657 34.6575 26.9756 34.6575 17.5001C34.6575 8.02465 26.9761 0.343262 17.5006 0.343262C8.02514 0.343262 0.34375 8.02465 0.34375 17.5001C0.34375 26.9756 8.02514 34.657 17.5006 34.657Z" stroke="white" stroke-width="1.0" stroke-miterlimit="10"></path>
|
||
</g>
|
||
<defs>
|
||
<clipPath id="clip0_1717_1101">
|
||
<rect width="35" height="35" fill="white"></rect>
|
||
</clipPath>
|
||
</defs>
|
||
</svg>
|
||
</a></li>
|
||
</ul>
|
||
</div>
|
||
<div class="col-lg-3 col-12">
|
||
<p class="address_footer text-white">National Library of Medicine<br />
|
||
<a href="https://www.google.com/maps/place/8600+Rockville+Pike,+Bethesda,+MD+20894/@38.9959508,-77.101021,17z/data=!3m1!4b1!4m5!3m4!1s0x89b7c95e25765ddb:0x19156f88b27635b8!8m2!3d38.9959508!4d-77.0988323" class="text-white" target="_blank" rel="noopener noreferrer">8600 Rockville Pike<br />
|
||
Bethesda, MD 20894</a></p>
|
||
</div>
|
||
<div class="col-lg-3 col-12 centered-lg">
|
||
<p><a href="https://www.nlm.nih.gov/web_policies.html" class="text-white">Web Policies</a><br />
|
||
<a href="https://www.nih.gov/institutes-nih/nih-office-director/office-communications-public-liaison/freedom-information-act-office" class="text-white">FOIA</a><br />
|
||
<a href="https://www.hhs.gov/vulnerability-disclosure-policy/index.html" class="text-white" id="vdp">HHS Vulnerability Disclosure</a></p>
|
||
</div>
|
||
<div class="col-lg-3 col-12 centered-lg">
|
||
<p><a class="supportLink text-white" href="https://support.nlm.nih.gov/">Help</a><br />
|
||
<a href="https://www.nlm.nih.gov/accessibility.html" class="text-white">Accessibility</a><br />
|
||
<a href="https://www.nlm.nih.gov/careers/careers.html" class="text-white">Careers</a></p>
|
||
</div>
|
||
</div>
|
||
<div class="row">
|
||
<div class="col-lg-12 centered-lg">
|
||
<nav class="bottom-links">
|
||
<ul class="mt-3">
|
||
<li>
|
||
<a class="text-white" href="//www.nlm.nih.gov/">NLM</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.nih.gov/">NIH</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.hhs.gov/">HHS</a>
|
||
</li>
|
||
<li>
|
||
<a class="text-white" href="https://www.usa.gov/">USA.gov</a>
|
||
</li>
|
||
</ul>
|
||
</nav>
|
||
</div>
|
||
</div>
|
||
</div>
|
||
</section>
|
||
<script type="text/javascript" src="/portal/portal3rc.fcgi/rlib/js/InstrumentOmnitureBaseJS/InstrumentNCBIConfigJS/InstrumentNCBIBaseJS/InstrumentPageStarterJS.js?v=1"> </script>
|
||
<script type="text/javascript" src="/portal/portal3rc.fcgi/static/js/hfjs2.js"> </script>
|
||
</div>
|
||
</div>
|
||
<!--/.footer-->
|
||
<p class="last-updated small">Last updated: 2024-10-09T12:38:06Z</p>
|
||
</div>
|
||
<!--/.page-->
|
||
</div>
|
||
<!--/.wrap-->
|
||
<span class="PAFAppResources"></span>
|
||
|
||
|
||
</div><!-- /.twelve_col -->
|
||
</div>
|
||
<!-- /.grid -->
|
||
|
||
|
||
|
||
<!-- usually for JS scripts at page bottom -->
|
||
<span class="pagefixtures"></span>
|
||
|
||
|
||
<!-- CE8B5AF87C7FFCB1_0191SID /projects/staticsites/genbank/genbank@2.21 portal104 v4.1.r689238 Tue, Oct 22 2024 16:10:51 -->
|
||
<span id="portal-csrf-token" style="display:none" data-token="CE8B5AF87C7FFCB1_0191SID"></span>
|
||
|
||
<script type="text/javascript" src="//static.pubmed.gov/portal/portal3rc.fcgi/4218137/js/3879255/4121861/1490097/4087685.js" snapshot="genbank"></script></body>
|
||
</html>
|
||
|