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<meta name="keywords" content="Vector Alignment Search Tool (VAST), 3D macromolecular structures, three-dimensional structures, 3D, 3-D, structural alignments, similar structures, superpose, superpositions, superimpose, superimposition, proteins, chemicals, nucleotide sequences, x-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, nucleic acids, DNA, RNA, ligands, bound chemicals, drugs, medicine, Entrez Structure, Molecular Modeling Database (MMDB), VAST+, Conserved Domains Database (CDD), CDTree, CD Tree, PubChem, NCBI, National Center for Biotechnology Information" />
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<TD class="DocTitleText" align="left"> <A HREF="../vast.shtml">VAST: <SPAN class="DocTitleTextSmall13">Vector Alignment Search Tool Help Document</SPAN></A></TD>
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<!-- TD class="NavResourceButtonInactive" align="center" valign="center" style="white-space: nowrap;"><A href="../MMDB/docs/mmdb_how_to.html#VAST"> HOW TO </A></TD -->
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<!-- TD class="NavResourceButtonInactive" align="center" valign="center" style="white-space: nowrap;"><A href="vastsrchelp.html"> VAST SEARCH HELP </A></TD -->
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<TD class="NavResourceButtonInactive" align="center" valign="center" style="white-space: nowrap;"><A HREF="../MMDB/mmdb.shtml" title="Overview of macromolecular resources available from the NCBI Structure Group"> RESOURCES </A></TD>
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<TD class="NavResourceButtonInactive" align="center" valign="center" style="white-space: nowrap;"><A href="../MMDB/docs/mmdb_news.html" title="News about the macromolecular resources available from the NCBI Structure Group"> NEWS </A></TD>
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<TD class="NavResourceButtonInactive" align="center" valign="center" style="white-space: nowrap;"><A HREF="../structure_discover.html" title="Discover associations among protein sequence, function, and structure"> DISCOVER </A></TD>
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<P class="indent20bottomspace">This <SPAN style="background-color: #FFFF00"><A HREF="vasthelp.html"><B>VAST help document</B></A></SPAN> provides general information about <A HREF="#OriginalVAST"><B>original VAST</B></A>, which finds 3-dimensional similarities between individual protein molecules, or between individual <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains"><B>3D domains</B></A>. The <SPAN style="background-color: #FFFF00"><A HREF="../vastplus/docs/vastplus_help.html"><B>VAST+ help document</B></A></SPAN> desribes the newest version of NCBI's structure similarity search tool, <A HREF="../vastplus/vastplus.cgi"><B>VAST+</B></A>, which finds 3-dimensional similarities between macromolecular complexes (<A HREF="../vastplus/docs/vastplus_help.html#OutputBiologicalUnit"><B>biological units</B></A>). (Read more about the <A HREF="../vastplus/docs/vastplus_help.html#Compare"><B>differences between VAST and VAST+</B></A>.) The <SPAN style="background-color: #FFFF00"><A HREF="vastsrchelp.html"><B>VAST Search help document</B></A></SPAN> describes how to use the <A HREF="vastsearch.html"><B>VAST Search page</B></A>, which allows you to enter a query structure as a <A HREF="vastsrchelp.html#Submission"><B>PDB-formatted file</B></A>. <!-- including detailed descriptions of input required, output displays, and the program's features and functions. The "<A HREF="vast_how_to.html"><B>How To</B></A>" page provides <A HREF="vast_how_to.html"><B>quick start guides</B></A> that provide some examples of how VAST can be used.[<BR>Once records of interest are retrieved, follow Entrez's "Links" to <A HREF="../../structure_discover.html"><B>discover associations among previously disparate data</B></A>] --></P></TD>
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<!-- ########## BLUE_HEADER ########## -->
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<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP"><B>VAST Help</B></TD>
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<UL>
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<LI><A HREF="#VASTWhat">What is VAST?</A> <I>(<A HREF="#IllustrationLipocalins"><span style="color:#d70000">illustration</span></A>)</I></LI><BR>
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<LI><A HREF="#VASTHow">How can I find similar structures for a structure that is already in the Molecular Modeling Database (MMDB)?</A></LI><BR>
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<LI><A HREF="#VASTSearchHow">How can I compare a newly resolved 3D structure against all of the structures in the Molecular Modeling Database (MMDB)?</A></LI><BR>
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<LI><A HREF="#OriginalVASTDisplay">What information is displayed on an "original VAST" results page?</A> <I>(<A HREF="#OriginalVASTDisplayIllustration"><span style="color:#d70000">illustration</span></A>)</I></LI><BR>
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<LI><A HREF="#VASTGraph">What does the "Graphics" display show?</A> <I>(<A HREF="#VASTGraphIllustration"><span style="color:#d70000">illustration</span></A>)</I></LI><BR>
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<LI><A HREF="#VASTTable">What does the "Table" display show, and what VAST similarity measures are listed?</A></LI><BR>
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<LI><A HREF="#VASTNR">How are non-redundant subsets of protein chains selected?</A></LI><BR>
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<LI><A HREF="#VASTOther">How can I display a different subset of similar structures, or find a specific structure within my search results?</A></LI><BR>
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<LI><A HREF="#VASTView">How can I view or save a structure superposition?</A></LI><BR>
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<LI><A HREF="#VASTHtml">How can I display a sequence alignment created from a structure superposition?</A></LI><BR>
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<LI><A HREF="#VASTNonbr">What does it mean when it says VAST did not find any structure neighbors?</A></LI><BR>
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<!-- LI><A HREF="#______">__________</A></LI><BR>
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<LI><A HREF="#______">__________</A></LI -->
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<A NAME="VASTWhat"></A>
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<A NAME="WhatIs"></A>
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<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
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<B>What is VAST?</B></TD>
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<A HREF="vast.shtml"><B>VAST</B>, short for <B>V</B>ector <B>A</B>lignment <B>S</B>earch <B>T</B>ool</A>, is a computer algorithm developed at NCBI and used to identify similar protein 3-dimensional structures by purely geometric criteria, and to identify distant homologs that cannot be recognized by sequence comparison. The similar 3D structures identified by VAST are also referred to as "<B>structure neighbors</B>." Superpositions of the similar structures, and their corresponding sequence alignments, can be viewed interactively in NCBI's free <A HREF="../CN3D/cn3d.shtml"><B>Cn3D</B></A> structure viewing program.<BR><BR>
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<!-- The following article describes the VAST algorithm and provides examples:<BR -->
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<TD class="WhiteCell ReferenceText" ALIGN="Left" VALIGN="TOP" colspan="3"><A NAME="CitingVAST"></A><A HREF="https://www.ncbi.nlm.nih.gov/pubmed/8804824?dopt=AbstractPlus"><img border=0 align=left height=15 width=15 style="margin-right:0.2em" src="../IMG/PubMed.gif"></A><A HREF="https://www.ncbi.nlm.nih.gov/pubmed/8804824?dopt=AbstractPlus">Gibrat JF, Madej T, Bryant SH. Surprising similarities in structure comparison. <B><I>Curr Opin Struct Biol.</I> 1996</B> Jun; 6(3): 377-85. [PubMed]</A> <A HREF="__________">[Full Text]</A><BR><BR></TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP"><A NAME="IllustrationLipocalins"></A><A NAME="VASTWhatIllustration"></A><A NAME="WhatIsIllustration"></A>
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<B>Reference:</B><BR><BR>
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The following article describes the VAST algorithm and provides examples:<BR><BR>
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<SPAN style="ReferenceText"><A NAME="CitingVAST"></A><A HREF="https://www.ncbi.nlm.nih.gov/pubmed/8804824?dopt=AbstractPlus"><img border=0 align=left height=15 width=15 style="margin-right:0.2em" src="../IMG/PubMed.gif"></A><A HREF="https://www.ncbi.nlm.nih.gov/pubmed/8804824?dopt=AbstractPlus">Gibrat JF, Madej T, Bryant SH. Surprising similarities in structure comparison. <I>Curr Opin Struct Biol.</I> 1996 Jun; 6(3): 377-85. [PubMed]</A> <!-- A HREF="__________">[Full Text]</A --></SPAN><BR><BR>
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The <A HREF="/docs/vast_publications.html">VAST publications page</A> provides additional references, including information about citing VAST.<BR><BR>
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<B>Illustrated example of similar structures:</B><BR><BR>
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In addition to the examples provided in the reference, the illustration at the right shows a superposition of lipocalins from bacteria, insect, and human. Click on the image to open the interactive 3D alignment in the free Cn3D program. Please note that <A HREF="../CN3D/cn3d.shtml"><B>Cn3D</B></A> 4.3.1 must be installed in your computer in order for the file to open. The <A HREF="../CN3D/cn3dtut.shtml">Cn3D Tutorial</A> provides additional details about <A HREF="../CN3D/cn3dtutP4.shtml">viewing structure alignments in Cn3D</A>.<!-- For best results, use the newest version of the software, Cn3D 4.3.1. --><BR><BR>
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<B>Two versions of VAST:</B><BR><BR>
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The <B>original VAST</B>, described in this document, (1) lists the <!-- A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvMolecularComponents"><A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvProteins" -->protein molecules ("chains") in the query structure, and the <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains">3D domains</A> that were identified in each protein, and (2) retrieves structures that are similar in shape to any <B>individual protein molecule</B> or <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains"><B>3D domain</B></A></B> from the structure (<I><A HREF="#OriginalVASTIllustration"><span style="color:#d70000">illustrated example</span> of original VAST results</A></I>).<BR><BR>
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The newer <A HREF="../vastplus/vastplus.cgi"><B>VAST+</B></A> groups 3D-similar structures based on their degree of similarity (complete or partial) to the <A HREF="../vastplus/docs/vastplus_help.html#OutputBiologicalUnit"><B>biological unit</B></A> of the query structure, and <A HREF="../vastplus/docs/vastplus_help.html#OutputSortOptions"><B>ranks</B></A> them by the number of protein molecules in the query that simultaneously match the 3D shape of protein molecules in the VAST neighbor (<I><A HREF="../vastplus/docs/vastplus_help.html#IllustratedExamples"><span style="color:#d70000">illustrated examples</span> of VAST+ results</A></I>).<BR><BR>
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<B>Additional details:</B><BR><BR>
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The <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing">data processing</A>:<A HREF="../MMDB/docs/mmdb_help.html#DataProcessingGeometricalFeatures">geometrical features</A> section of the <A HREF="../MMDB/docs/mmdb_help.html">MMDB help document</A> provides more information about how the 3D domains and similar structures are identified.<BR><BR>
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The <A HREF="../vastplus/docs/vastplus_help.html">VAST+ help document</A> provides more information about the <A HREF="../vastplus/docs/vastplus_help.html#Compare"><B>difference between VAST and VAST+</B></A>.<BR><BR>
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<A HREF="docs/data/lipocalin_ecoli_2ACOB_tabacco_hornworm_1Z24A_human_2HZQA.cn3"><IMG SRC="docs/images/lipocalin_ecoli_2ACOB_tabacco_hornworm_1Z24A_human_2HZQA.png" WIDTH="500" HEIGHT="750" BORDER="0" ALT="Example 3D alignment of VAST similar structures, showing the ancient evolutionary relationship among lipocalins from bacteria, insects, and human. Click on the image to open the interactive 3D alignment in the free Cn3D program. Please note that Cn3D must be installed in your computer in order for the file to open."></A></TD>
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<TD class="WhiteCell MiniText" ALIGN="Center" VALIGN="TOP"> 
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<A NAME="VASTHow"></A>
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
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<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
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<B>How can I find similar structures for a structure that is already in the Molecular Modeling Database (MMDB)?</B></TD>
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</TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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<A HREF="vast.shtml"><B>VAST</B></A> is applied on every protein in the <A HREF="https://www.ncbi.nlm.nih.gov/structure/">Molecular Modeling Database (MMDB)</A> during <A HREF="../MMDB/docs/mmdb_help.html#DataProcessingGeometricalFeatures">MMDB data processing</A> in order to identify similar 3D structures. The <SPAN style="background-color: #FFFF00"><B>pre-computed results</B></SPAN> are accessible from a <A HREF="../MMDB/docs/mmdb_help.html#SummaryPage">structure's summary page</A>. To retrieve them, you can <B>use any one of the following methods</B>:
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<UL>
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<LI><A NAME="RetrievePrecomputedResults"></A>Enter the structure's <A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvMMDBID">MMDB ID</A> or <A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvPDBID">PDB ID</A> in the "<A HREF="vast.shtml#RetrievePrecomputedResults">Retrieve pre-computed results</A>" section of the <A HREF="vast.shtml"><B>VAST home page</B></A> and press "GO". By default, the results will be displayed in the new <A HREF="../vastplus/vastplus.cgi"><B>VAST+</B></A> display format, which focuses on similarities between the <B>macromolecular complexes</B> of the query structure and hits (<A HREF="../vastplus/docs/vastplus_help.html#IllustratedExamples"><I><span style="color:#d70000">illustrated examples</span></I></A>).<BR><BR>
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If you prefer to see the <A HREF="vasthelp.html#OriginalVASTDisplay">original-style VAST results</A>, which focus on similarities between <B>individual protein molecules or <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains">3D domains</A></B> within the query structure and hits, click on the <A HREF="../vastplus/docs/vastplus_help.html#OriginalVASTButton">"original VAST" button</A> near the upper right corner of the <A HREF="../vastplus/vastplus.cgi">VAST+</A> search results page. That will open a table which lists the <I>individual protein molecules</I> (and <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains"><I>3D domains</I></A>, if present) in your query structure (<A HREF="../vastplus/docs/vastplus_help.html#OriginalVASTDisplay"><I><span style="color:#d70000">illustrated example</span></I></A>). Click on any link beside a protein molecule or 3D domain of interest to view a list of structures that contain a similarly shaped protein molecule or 3D domain.<BR><BR>
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<!-- img SRC="../../IMG/spacer.gif" width="20" height="1" border="0" -->— <I>OR</I> —</LI><BR -->
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<!-- LI>Follow the link for via "<A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvSimilarStructures"><B>Similar Structures</B></A>" in the upper right corner of the page. The resulting VAST summary page will list the protein molecules in the structure and the 3D domains found in each protein [(<A HREF="..//MMDB/docs/mmdb_help.html#MmdbsrvSimilarStructures">illustrated example</A>)]. Select any protein molecule or 3D domain to open a list of structures that are similar in shape to the protein or 3D domain you selected.<BR><BR> OR</LI><BR><BR -->
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<LI>Retrieve a structure of interest from <A HREF="https://www.ncbi.nlm.nih.gov/structure"><B>MMDB</B></A> and open its <A HREF="../MMDB/docs/mmdb_help.html#SummaryPage">structure summary page</A>. Follow the "<B>Similar Structures: VAST+</B>" link near the upper right corner of a structure's MMDB structure summary page. That will open the new <A HREF="../vastplus/vastplus.cgi"><B>VAST+</B></A> search results page (<A HREF="../vastplus/docs/vastplus_help.html#IllustratedExamples"><I><span style="color:#d70000">illustrated examples</span></I></A>), which lists the query structure followed by similar structures, ranked by the degree of <B>similarity to the query structure's</B> <A HREF="../vastplus/docs/vastplus_help.html#OutputBiologicalUnit"><B>biological unit</B></A>.<BR><BR>
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If you prefer to see the <A HREF="vasthelp.html#OriginalVASTDisplay">original-style VAST results</A>, which focus on similarities between <B>individual protein molecules or <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains">3D domains</A></B> within the query structure and hits, click on the <A HREF="../vastplus/docs/vastplus_help.html#OriginalVASTButton">"original VAST" button</A> near the upper right corner of the <A HREF="../vastplus/vastplus.cgi">VAST+</A> search results page. That will open a table which lists the <I>individual protein molecules</I> (and <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains"><I>3D domains</I></A>, if present) in your query structure (<A HREF="../vastplus/docs/vastplus_help.html#OriginalVASTDisplay"><I><span style="color:#d70000">illustrated example</span></I></A>). Click on any link beside a protein molecule or 3D domain of interest to view a list of structures that contain a similarly shaped protein molecule or 3D domain.<BR><BR>
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<!-- img SRC="../../IMG/spacer.gif" width="20" height="1" border="0" -->— <I>OR</I> —</LI><BR>
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<LI>Retrieve a structure of interest from <A HREF="https://www.ncbi.nlm.nih.gov/structure"><B>MMDB</B></A> and open its <A HREF="../MMDB/docs/mmdb_help.html#SummaryPage">structure summary page</A>. View the "<A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvProteinsShowAnnotation"><B>show annotation</B></A>" graphic for any protein molecule of interest, then click on the bar graphic for the overall protein molecule or for any 3D domain it contains in order to view a list of other structures that are similar in shape to the molecule or 3D domain you selected.</LI>
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</UL>
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The <A HREF="../vastplus/docs/vastplus_help.html">VAST+ help document</A> provides details about the <A HREF="../vastplus/docs/vastplus_help.html#Compare">differences between VAST and VAST+</A>, as well as an <A HREF="../vastplus/docs/vastplus_help.html#OriginalVASTDisplay">illustrated example of original VAST results</A> and <A HREF="../vastplus/docs/vastplus_help.html#IllustratedExamples">illustrated examples of VAST+ results</A>.)<BR><BR>
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<I>Note: If you have a <SPAN style="background-color: #FFFF00"><B>newly resolved protein structure</B></SPAN> that is not yet in MMDB, you can use the <A HREF="vastsearch.html"><B>VAST Search</B></A> service to <A HREF="vastsrchelp.html#Submission">input</A> your data in <!-- A HREF="http://www.wwpdb.org/docs.html" --><A HREF="http://www.wwpdb.org/documentation/file-format"><B>PDB file format</B></A> and compare your structure against all those in MMDB. See details in the FAQ below, on "<A HREF="#VASTSearchHow">How can I compare a newly resolved 3D structure against all of the structures in the Molecular Modeling Database (MMDB)?</A>"</I><BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
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<img src="________.png" width="500" height="100"><BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR -->
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<TD class="WhiteCell MiniText" ALIGN="Center" VALIGN="TOP"> </TD>
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<!-- ==================== VERTICAL SPACER ======================= -->
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<TD class="WhiteCell NormalText"> </TD>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ======================== BOX4 =========================== -->
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<A NAME="VASTSearchHow"></A>
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
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<TR>
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<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
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<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
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<B>How can I compare a newly resolved 3D structure against all of the structures in the Molecular Modeling Database (MMDB)?</B></TD>
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</TR>
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<!-- ########## END_BLUE_HEADER ########## -->
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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If you have a newly determined protein structures that is not yet in MMDB, then you can use the <A HREF="vastsearch.html"><B>VAST Search</B></A> service to <A HREF="vastsrchelp.html#Submission">input</A> your data in <!-- A HREF="http://www.wwpdb.org/docs.html" --><A HREF="http://www.wwpdb.org/documentation/file-format"><B>PDB file format</B></A> and compare your structure against all those in MMDB. The <A HREF="vastsrchelp.html"><B>VAST Search Help</B></A> document provides additional information about using the VAST Search page.<BR><BR>
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Please note that, at this time, <A HREF="vastsearch.html">VAST Search</A> still returns results in the <A HREF="vasthelp.html#OriginalVASTDisplay">original-style VAST display</A>, which lists structures that have similarities to <B>individual protein molecules</B> and <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains"><B>3D domains</B></A> in your query structure, and allows you to view their sequence alignments and 3D superpositions.<BR><BR>
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The new <A HREF="../vastplus/vastplus.cgi">VAST+</A> search results, which rank similar structures based on their <A HREF="../vastplus/docs/vastplus_help.html#OutputBiologicalUnit">biological unit</A> similarity to the query structure, and provide the ability to view sequence alignments and superpositions of the biological units, are currently calculated only for structures that are available in the public database.<!-- The original style VAST results does not rank results by biological unit similarity. --><BR><BR>
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The <A HREF="../vastplus/docs/vastplus_help.html">VAST+ help document</A> provides details about the <A HREF="../vastplus/docs/vastplus_help.html#Compare">differences between VAST and VAST+</A>, as well as an <A HREF="../vastplus/docs/vastplus_help.html#OriginalVASTDisplay">illustrated example of original VAST results</A> and <A HREF="../vastplus/docs/vastplus_help.html#IllustratedExamples">illustrated examples of VAST+ results</A>.<BR><BR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<!-- TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
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<img src="________.png" width="500" height="100"><BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR -->
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<TD class="WhiteCell MiniText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TABLE>
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<!-- ==================== VERTICAL SPACER ======================= -->
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<!-- ======================== BOX5 =========================== -->
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<A NAME="VASTMoleculeList"></A>
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<A NAME="OriginalVAST"></A>
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<A NAME="OriginalVASTDisplay"></A>
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
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<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
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<B>What information is displayed on an "original VAST" results page?</B></TD>
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</TR>
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<!-- ########## END_BLUE_HEADER ########## -->
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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Whether you retrieve similar structures for a <A HREF="#VASTSearchHow">newly resolved structure</A>, or follow the "similar structures" link in a <A HREF="#VASTHow">structure record that's available in the public database</A>, the <B>original style VAST display</B> will provide the following information:<BR>
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<UL>
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<LI>list of the <!-- A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvMolecularComponents"><A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvProteins" --><B>protein molecules</B> ("chains") in the query structure, and the <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains"><B>3D domains</B></A> that were identified by the VAST algorithm in each protein</LI><BR>
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<LI>list of the structures that are similar in shape to any <B><I>individual</I> protein molecule or 3D domain</B> of your query structure, with links to views of their sequence alignments and 3D superpositions to the query structure.</LI>
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</UL>
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An <B>example</B> of the <B>original style VAST display</B> is <A HREF="#OriginalVASTIllustration"><span style="color:#d70000"><I>illustrated below</I></A>.<BR>
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<UL>
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<LI><B>Part 1</B> of the illustration shows <B>colored bars</B> that represent the <B>compact substructures</B>, or <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains"><B>3D domains</B></A>, detected by VAST in the query structure's protein molecule(s). These 3D domains serve as the fundamental unit of structure comparison. (The <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing">data processing</A>:<A HREF="../MMDB/docs/mmdb_help.html#DataProcessingGeometricalFeatures">geometrical features</A> section of the MMDB help document provides more information about how the 3D domains and similar structures are identified.) To view the 3D domains that have been identified in a protein molecule, open the MMDB <A HREF="../MMDB/docs/mmdb_help.html#SummaryPage">structure summary</A> page for a structure of interest, sroll down to the table of <A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvMolecularComponents">molecules and interactions</A>, and click on the "<A HREF="../MMDB/docs/mmdb_help.html#MmdbsrvProteinsShowAnnotation">show annotation</A>" link for the protein of interest. (For example, open the <A HREF="https://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=1PTH">structure summary page for 1PTH</A>.)</LI><BR>
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<LI><B>Part 2</B> of the illustration shows the original-style VAST display, which consists of a <B>table summarizing the protein molecules and <A HREF="../MMDB/docs/mmdb_help.html#DataProcessing3dDomains">3D domains</A></B> in the query structure, and the number of structures that are geometrically similar to each individual protein molecule or 3D domain in your query structure. For any protein molecule or 3D domain of interest, click on the link in the "# of Related Structures" column to view a list of similar structures. The list includes a graphical display of alignment footprints and provides options to view sequence alignments and superposed structures.<!-- Original VAST does <B>not</B> group the results based on degree of similarity to the <A HREF="#OutputBiologicalUnit">biological unit</A> of the query structure. --></LI>
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</UL>
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To explore the example interactively, you can <A HREF="https://www.ncbi.nlm.nih.gov/Structure/vastplus/vastplus.cgi?uid=1PTH&style=old">open a live web page with original-style VAST results for 1PTH</A>, or
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click on the image below to open an <A HREF="../MMDB/docs/data/vast_alignment_of_1PTH_A_and_1EQG_A.cn3">interactive view of the 3D alignment of 1PTH's protein A, domain 1 and a sample similar structure, 1EQG</A> (Ovine Cox-1 Complexed With Ibuprofen). (Please note that <A HREF="../CN3D/cn3d.shtml">Cn3D</A> 4.3.1 must be installed in your computer in order for the file to open. The <A HREF="../CN3D/cn3dtut.shtml">Cn3D Tutorial</A> provides additional details about <A HREF="../CN3D/cn3dtutP4.shtml">viewing structure alignments in Cn3D</A>.)<BR><BR>
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<!-- A HREF="https://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=1PTH">1PTH</A -->
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<!-- A HREF="https://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=1EQG">1EQG</A -->
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The original style VAST display is still accessible by clicking the <B>"Original VAST" button</B> <!-- in the grey "Similar Structures" header bar at --> near the top of a VAST+ search results display<!-- or by the <A HREF="../../VAST/vasthelp.html#VASTHow">additional methods</A> described in the <A HREF="../../VAST/vasthelp.html">original VAST help document</A -->. This document describes the features, functions, and graphics of the original style VAST display.<BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- ========= IMAGE_OF_INITIAL_VAST_RESULTS_DISPLAY =========== -->
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
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<A NAME="OriginalVASTDisplayIllustration"></A>
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<A NAME="OriginalVASTIllustration"></A>
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="5" class="WhiteCell NormalText3">
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<TR>
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<TD class="WhiteCell" WIDTH="10" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCellBlueEdgeAll" ALIGN="Left" VALIGN="TOP">
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<P class="indent20bottomspace NormalText3"><B>Example</B> - Original style VAST display (as of 07 November 2013) for <A HREF="https://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=50885"><B>1PTH</B></A>, "The Structural Basis of Aspirin Activity Inferred From the Crystal Structure of Inactivated Prostaglandin H2 Synthase" (sheep prostaglandin H2 synthase)<BR><BR>
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<A HREF="../MMDB/docs/data/vast_alignment_of_1PTH_A_and_1EQG_A.cn3"><IMG SRC="../vastplus/docs/images/1PTH_structure_summary_vast_related_structures_individual_chains.png" WIDTH="800" HEIGHT="800" BORDER="0" ALT="Illustration of the compact substructures, called 3D domains, in the structure for 1PTH, sheep prostaglandin H2 synthase, and the list of Similar Structures that have a similar shape to the whole protein molecule or to any 3D domain within it. Click anywhere on this image to open an interactive view of the 3D alignment of protein A, domain 1, in 1PTH and a sample similar structure, 1EQG (Ovine Cox-1 Complexed With Ibuprofen). Install Cn3D before clicking, if that program is not yet on your computer."></A><BR><BR>
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<I><A HREF="https://www.ncbi.nlm.nih.gov/Structure/vastplus/vastplus.cgi?uid=1PTH&style=old">Open a live web page with original-style VAST results for 1PTH.</A></I><BR>
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<!-- BR><A HREF="https://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=88951"><B>3O6F</B></A> Crystal Structure of a Human Autoimmune TCR Ms2-3c8 Bound to MHC Class II Self-ligand Mbphla-dr4:<BR><BR>
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<IMG SRC="images/______" WIDTH="600" HEIGHT="200" BORDER="0" ALT="________"><BR>
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<A HREF="https://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=88951&ShowOp=VastSum">open a live web page with original-style VAST results for 3O6F</A --></P>
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</TD>
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<TD class="WhiteCell" WIDTH="5" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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</TABLE>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- ========= END_IMAGE_OF_INITIAL_VAST_RESULTS_DISPLAY =========== -->
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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<BLOCKQUOTE><I>In contrast to the original VAST display shown above, which focuses on similarities between individual protein molecules or 3D domains, the newer <A HREF="../vastplus/docs/vastplus_help.html#Output"><B>VAST+ display</B></A> groups 3D-similar structures based on their degree of similarity (complete or partial) to the <B>macromolecular complex</B> (<A HREF="../vastplus/docs/vastplus_help.html#OutputBiologicalUnit">biological unit</A>) of the query structure; <A HREF="../vastplus/docs/vastplus_help.html#OutputSortOptions">ranks</A> them by the number of protein molecules in the query that simultaneously match the 3D shape of protein molecules in the VAST neighbor; and enables you to view the <A HREF="../vastplus/docs/vastplus_help.html#SeqAlignView">sequence alignments</A> and <A HREF="../vastplus/docs/vastplus_help.html#3DView">3D superpositions</A> of the biological units. The <A HREF="../vastplus/docs/vastplus_help.html">VAST+ help document</A> provides <A HREF="../vastplus/docs/vastplus_help.html#IllustratedExamples"><span style="color:#d70000">illustrated examples</span> of VAST+ results</A> and additional details about the <A HREF="../vastplus/docs/vastplus_help.html#Compare">difference between VAST and VAST+</A>.</I> </BLOCKQUOTE>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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</TABLE>
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</TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> 
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<BR> <BR></TD>
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</TR>
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</TABLE>
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<!-- ======================== END_BOX5 =========================== -->
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<!-- ==================== VERTICAL SPACER ======================= -->
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<TR>
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<TD class="WhiteCell NormalText"> </TD>
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</TR>
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</TABLE>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ======================== BOX6 =========================== -->
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<A NAME="VASTGraph"></A>
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
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<!-- ########## BLUE_HEADER ########## -->
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<TR>
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<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
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<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
|
|
<B>What does the "Graphics" display show?</B></TD>
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</TR>
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<!-- ########## END_BLUE_HEADER ########## -->
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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After you select a protein molecule ("chain") or 3D domain of interest from the <A HREF="#VASTMoleculeList">initial VAST results page</A>, you will see a brief list of structures that are similar in shape to the protein molecule or 3D domain you selected.<BR><BR>
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By <B>default</B>, the results are shown as a <B>Graphics</B> display (illustrated below) and list only a <B>"medium <A HREF="#VASTNR">redundancy</A>" subset</B> of structure neighbors, with red bars representing the alignment footprint of each structure neighbor relative to the query protein. (More details about the display are provided below the illustrated example.)<BR><BR>
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<B>Controls</B> near the top of the page allow you to change to a <A HREF="#VASTTable"><B>Table</B></A> display (descibed in the next section of this document), and/or to increase or decrease the number of hits shown on the VAST results page with options that range from a "<B>low redundancy</B>" subset of proteins from structure records to "<B>all sequences</B>." After you select the desired options, be sure to press the <B>"List" button</B> in order to refresh the display.<BR><BR>
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The similar structures can be displayed as <B>Graphics</B> (illustrated below) or as a <A HREF="#VASTTable"><B>Table</B></A> (descibed in the next section of this document).<BR><BR></TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- ========= IMAGE_OF_VAST_RESULTS_GRAPHIC_DISPLAY =========== -->
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
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<A NAME="VASTGraphIllustration">
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|
<IMG SRC="docs/images/1PTH_A_vast_results_graphics_display.png" WIDTH="600" HEIGHT="475" BORDER="1" ALT="Illustration showing the default VAST results graphic display for the sheep prostaglandin H2 synthase-1 protein from the 1PTH structure record."><BR><BR>
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</TD>
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- ========= END_IMAGE_OF_VAST_RESULTS_GRAPHIC_DISPLAY =========== -->
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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The <B>identifier</B> for each structure neighbor is shown in the format of PDB ID + protein chain ID + 3D domain ID (e.g., 1Q4G A 4, which represents domain 4 in protein chain A from the 1Q4G structure record).<BR><BR>
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The <B>red bars</B> indicate the region/residues of the query domain that can be superimposed on residues from each neighbor. The gray bars and blank space are unaligned regions. These region colors are the same as those shown in <a href="../CN3D/cn3d.shtml"><B>Cn3D</B></a> when a <A HREF="#VASTView"><!-- A HREF="../CN3D/cn3dtutP4.shtml" --><B>structure superposition</B></A> is viewed in Cn3D. When the mouse is over each icon, it will display a description of what it represents.<BR><BR>
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On the <B>sequence ruler</B> next to the query protein ("1PTH A" in the illustration above), the aligned region indicates a sum of regions from all neighbors. This indicates the maximum fragment in the query that is similar to some other structures. The individual <B>3D domains</B> in the chain are indicated by rectangles below the sequence ruler with different colors and numbers. MMDB's 3D domains are defined on the basis of structural compactness. Red indicates the query domain. Links to the <B>conserved domain</B> database are provided for convenience, to provide names and descriptions (where possible) of the 3D domains to which they correspond.<BR><BR>
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The <B>check box</B> at the left hand side of a structure neighbor's "row"
|
|
allows for selection of individual neighbors and their 3D superposition.
|
|
Clicking the sequence identifier beside it will go to the Entrez sequence page
|
|
of the neighbor. The red aligned regions in a neighbor's sequence are
|
|
displayed at the positions of their equivalent residues in the query sequence.
|
|
Clicking on these will display an HTML view of the sequence
|
|
alignment between the query and the neighbor. One of the VAST similarity
|
|
measures used for sorting (here, the <B>alignment length</B>: e.g., 551 residues
|
|
residues from 1PTH_A are aligned with 1Q4GA) is listed at the right hand side of the row. Clicking the name of the similarity measure (i.e., "Ali_len" in our example) will display a table with all of the VAST statistics.
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</TD>
|
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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</TABLE>
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</TD>
|
|
</TR>
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<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> 
|
|
<BR> <BR></TD>
|
|
</TR>
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</TABLE>
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|
|
<!-- ======================== END_BOX6 =========================== -->
|
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<!-- ==================== VERTICAL SPACER ======================= -->
|
|
|
|
<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
|
|
<TR>
|
|
<TD class="WhiteCell NormalText"> </TD>
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|
</TR>
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</TABLE>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ======================== BOX7 =========================== -->
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<A NAME="VASTTable"></A>
|
|
<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
|
|
|
|
<!-- ########## BLUE_HEADER ########## -->
|
|
<TR>
|
|
<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
|
|
<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
|
|
<B>What does the "Table" display show, and what VAST similarity measures are listed?</B></TD>
|
|
</TR>
|
|
<!-- ########## END_BLUE_HEADER ########## -->
|
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|
<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
</TR>
|
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|
|
<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
|
|
|
|
<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
|
|
|
|
<TR>
|
|
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
|
|
<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
|
|
|
|
The display controls at the top of a VAST results page allow you to change the display from the default "<A HREF="#VASTGraph">Graphics</A>" format (described in the previous section) to a "<B>Table</B>" format.<BR><BR>
|
|
|
|
The "Table" display lists the <B>identifier</B> for each structure neighbor in the format of PDB ID + protein chain ID + 3D domain ID (e.g., 1Q4G A 4, which represents domain 4 in protein chain A from the 1Q4G structure record), its <B>description</B>, and a number of <B>measures of structural similarity</B>. The <B>columns</B> in the table include:<BR><BR>
|
|
|
|
<UL>
|
|
|
|
<LI><strong>Check box:</strong> Allow you to select the structure neighbors you'd like to view in a <A HREF="#VASTView">3D alignment</A> with the query protein structure.</LI>
|
|
|
|
<LI><strong>PDB:</strong> The four-character <a href="http://www.rcsb.org">PDB</a>-Identifier of the structure neighbor. Click on the Identifier to switch to the MMDB Summary page of the respective neighbor.</LI>
|
|
|
|
<LI><strong>C:</strong> The <a href="http://www.rcsb.org">PDB</a> chain name. A blank space indicates that the chain does not have an identifier (many protein structures have a single chain only). Note that non-alphanumeric characters such as dashes, hyphens, underscores, etc. may be used as chain names by <a href="http://www.rcsb.org">PDB</a>.</LI>
|
|
|
|
<LI><strong>D:</strong> The MMDB 3D domain identifier. Domains are parsed based on geometrical criteria (the ratio of intradomain contacts to interdomain contacts) by an automatic method and can be visualized with <a href="../CN3D/cn3d.shtml">Cn3D</a>.</LI>
|
|
|
|
<LI><strong>Aligned Length:</strong> The number of equivalent pairs of C-alpha atoms superimposed between the two structures, i.e. how many residues have been used to calculate the 3D superposition.</LI>
|
|
|
|
<LI><strong>SCORE:</strong> The VAST structure-similarity score. This number is related to the number of secondary structure elements superimposed and the quality of that superposition. Higher VAST scores correlate with higher similarity.</LI>
|
|
|
|
<LI><strong>P-VAL:</strong> The VAST <i>p</i> value is a measure of the significance of the comparison, expressed as a probability. For example, if the <i>p</i> value is 0.001, then the odds are 1000 to 1 against seeing a match of this quality by pure chance. The <i>p</i> value from VAST is adjusted for the effects of multiple comparisons using the assumption that there are 500 independent and unrelated types of domains in the MMDB database. The <i>p</i> value shown thus corresponds to the <i>p</i> value for the pairwise comparison of each domain pair, divided by 500.</LI>
|
|
|
|
<LI><strong>RMSD:</strong> The root mean square superposition residual in Angstroms. This number is calculated after optimal superposition of two structures, as the square root of the mean square distances between equivalent C-alpha atoms. Note that the RMSD value scales with the extent of the structural alignments and that this size must be taken into consideration when using RMSD as a descriptor of overall structural similarity.</LI>
|
|
|
|
<LI><strong>%Id: </strong> Percent identical residues in the aligned sequence region. This is a raw measure of sequence similarity in the parts of the proteins that have been superimposed.</LI>
|
|
|
|
<LI><strong>LHM:</strong> Loop Hausdorff Metric. A Loop Similarity measure that shows how well two structures conform to each other in the loop regions, after structural superposition. The "loop regions" are the parts of the structures between aligned secondary structure elements (helices and strands). LHM is measured in Angstroms, with a smaller value indicative of greater similarity. The loop similarity may be undefined (indicated by 'NA') if there are too many residues with missing coordinates in the loops. Citation: <a HREF="https://www.ncbi.nlm.nih.gov/pubmed/15382231">Analysis of protein homology by assessing the (dis)similarity in protein loop regions</a></LI>
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|
|
|
<LI><strong>GSP:</strong> Gapped Score. A combination (algebraic) score that uses RMSD, aligned length, and the number of gapped regions in the alignment. A smaller gapped score correlates with greater similarity. Citation: <a HREF="https://www.ncbi.nlm.nih.gov/pubmed/15701525">Comprehensive evaluation of protein structure alignment methods: scoring by geometric measures.</a></LI>
|
|
|
|
<LI><strong>Description:</strong> A string parsed out of <a href="http://www.rcsb.org">PDB</a>'s COMPOUND records that describes the nature of the structure neighbor.</LI>
|
|
|
|
</UL>
|
|
|
|
</TD>
|
|
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
|
|
</TR>
|
|
|
|
<!-- TR>
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
|
|
<img src="________.png" width="500" height="100"><BR><BR>
|
|
</TD>
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
</TR -->
|
|
|
|
</TABLE>
|
|
|
|
</TD>
|
|
</TR>
|
|
|
|
<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
</TR>
|
|
|
|
</TABLE>
|
|
|
|
<!-- ======================== END_BOX7 =========================== -->
|
|
|
|
<!-- ==================== VERTICAL SPACER ======================= -->
|
|
|
|
<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
|
|
<TR>
|
|
<TD class="WhiteCell NormalText"> </TD>
|
|
</TR>
|
|
</TABLE>
|
|
|
|
<!-- ================== END_VERTICAL SPACER ===================== -->
|
|
|
|
<!-- ======================== BOX8 =========================== -->
|
|
|
|
<A NAME="VASTNR"></A>
|
|
<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
|
|
|
|
<!-- ########## BLUE_HEADER ########## -->
|
|
<TR>
|
|
<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
|
|
<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
|
|
<B>How are non-redundant subsets of protein chains selected?</B></TD>
|
|
</TR>
|
|
<!-- ########## END_BLUE_HEADER ########## -->
|
|
|
|
<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
</TR>
|
|
|
|
<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
|
|
|
|
<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
|
|
|
|
<TR>
|
|
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
|
|
<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
|
|
|
|
<a HREF="https://www.ncbi.nlm.nih.gov/structure">MMDB</a> chains are clustered into groups according to their amino acid sequence similarity in pairwise comparisons. A representative chain is selected from each group to compile a non-redundant subset of MMDB, and only one representative of each group is shown in a neighbor-list calculated by <a HREF="https://www.ncbi.nlm.nih.gov/Structure/VAST/vast.html">VAST</a>. By default, a lower level of redundancy at 10e-40 is used to report structure neighbors. This keeps the table shorter while providing the most informative summary of structural relationships in MMDB.<BR><BR>
|
|
|
|
All-against-all pairwise comparisons of MMDB-domains are calculated with the <a href="https://blast.ncbi.nlm.nih.gov/">BLAST</a> algorithm, setting a fixed database size parameter of 500,000 residues. Sequences are then clustered into groups by single linkage, whereby a sequence is merged into a group if it shows a BLAST <i>p</i> value of C or less with any member of the group. There are 5 levels of redundancy defined in MMDB database:
|
|
|
|
<OL>
|
|
<LI><strong>Low redundancy:</strong> representatives are chosen from each group where sequences show a BLAST <i>p</i> value of 10e-7 to each other</LI>
|
|
<LI><strong>Medium redundancy:</strong> representatives are chosen from each group where sequences show a BLAST <i>p</i> value of 10e-40 to each other</LI>
|
|
<LI><strong>High redundancy:</strong> representatives are chosen from each group where sequences show a BLAST <i>p</i> value of 10e-80 to each other</LI>
|
|
<LI><strong>Non-identical sequence level:</strong> representatives are chosen from each group where sequences are not identical to each other</LI>
|
|
<LI><strong>All sequences level:</strong> this is the most redundant level, which includes all of MMDB sequences</LI>
|
|
</OL>
|
|
|
|
Within each cluster of similar protein chains, cluster members are ranked
|
|
according to the apparent quality and completeness of the structure data.
|
|
The following criteria are used (ranked by decreasing priority):
|
|
|
|
<OL>
|
|
<LI>Low fraction of residues with unknown residue type</LI>
|
|
<LI>Low fraction of residues with incomplete coordinates</LI>
|
|
<LI>Low fraction of residues with missing coordinates</LI>
|
|
<LI>Low fraction of residues with incomplete side-chain coordinates</LI>
|
|
<LI>High resolution</LI>
|
|
<LI>High number of chains (subunits) contained in the PDB entry</LI>
|
|
<LI>High number of heterogens contained in the PDB entry</LI>
|
|
<LI>High number of different types of heterogens.</LI>
|
|
<LI>Chain length</LI>
|
|
</OL>
|
|
|
|
For the display of structure neighbors calculated by VAST, the highest ranking chain (according to the criteria above) from each cluster found in the list of neighbors is reported. In most cases this implies that the parent structure is also similar to the other members of the sequence redundant cluster. To have them displayed, the user must select a higher level of redundancy.
|
|
|
|
</TD>
|
|
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
|
|
</TR>
|
|
|
|
<!-- TR>
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
|
|
<img src="________.png" width="500" height="100"><BR><BR>
|
|
</TD>
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
</TR -->
|
|
|
|
</TABLE>
|
|
|
|
</TD>
|
|
</TR>
|
|
|
|
<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> <BR> <BR></TD>
|
|
</TR>
|
|
|
|
</TABLE>
|
|
|
|
<!-- ======================== END_BOX8 =========================== -->
|
|
|
|
<!-- ==================== VERTICAL SPACER ======================= -->
|
|
|
|
<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
|
|
<TR>
|
|
<TD class="WhiteCell NormalText"> </TD>
|
|
</TR>
|
|
</TABLE>
|
|
|
|
<!-- ================== END_VERTICAL SPACER ===================== -->
|
|
|
|
<!-- ======================== BOX9 =========================== -->
|
|
|
|
<A NAME="VASTOther"></A>
|
|
<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
|
|
|
|
<!-- ########## BLUE_HEADER ########## -->
|
|
<TR>
|
|
<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
|
|
<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
|
|
<B>How can I display a different subset of similar structures, or find a specific structure within my search results?</B></TD>
|
|
</TR>
|
|
<!-- ########## END_BLUE_HEADER ########## -->
|
|
|
|
<TR>
|
|
<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
|
|
</TR>
|
|
|
|
<TR>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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The <B>display controls</B> at the top of a VAST results page allow you to change the appearance of display from <A HREF="#VASTGraph"><B>Graphics</B></A> to <A HREF="#VASTTable"><B>Table</B></A> format. The graphic is helpful to understand the superpositions between a query domain and its neighbors. The table is good for viewing or saving the statistics from a VAST calculation.<BR><BR>
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The VAST <A HREF="#VASTTable"><B>similarity measures</B></A> reported for each neighbor can be used to determine sort order. The lengths of the whole graphic and table are strongly influenced by the display subset, which determines the level of sequence redundancy chosen.<BR><BR>
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The display controls also allow you to change the <B>number of structure neighbors listed</B> in the display. A brief <B>subset</B> of structure neighbors is shown by default. You can choose <B>increase or decrease</B> the number of hits shown on the VAST results page by using the <B>"List" options</B>, which range from a "<B>low redundancy</B>" subset of proteins from structure records to "<B>all sequences</B>."<BR><BR>
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The total number of neighbors displayed in a page is limited. At most 60
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neighbors from a non-redundant subset can be displayed simultaneously on one
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page. In addition, by clicking check boxes to select from previously listed
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neighbors, at most another 40 neighbors can also be displayed in the same
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page. Therefore the maximum capacity of one page is 100 neighbors. This
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feature, together with the pagination, is able to keep interesting neighbors from different pages displayed together. The page can be selected from the third pull-down menu in the "List" line.<BR><BR>
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The "<B>Advanced similar structure search</B>" options allow you to search for specific structures in your current set of search results. For example, if you know that a particular structure should be in your VAST results but you don't see it in the currently displayed subset of hits, you can use the <B>"Find" button</B> to look for that structure by MMDB, PDB, or 3D-Domain identifier.<!-- If you press the "Find" button but do not enter any identifiers, the display will be refreshed to show only those structure neighbors that were selected previously. If no structure neighbors were selected and no identifiers were entered before pressing the "Find" button, an error message will be displayed. --> If you have done a previous search in the <A HREF="https://www.ncbi.nlm.nih.gov/structure/">Entrez Structure (MMDB)</A> database and want to find out if any of the structures retrieved by that search are in the current VAST results, you can use the "Entrez History" function in the "Advanced similar structure search" panel. That will show you the intersection, if any, of the previous Entrez Structure and current VAST search results.<BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
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<img src="________.png" width="500" height="100"><BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR -->
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</TABLE>
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</TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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</TABLE>
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<!-- ======================== END_BOX9 =========================== -->
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<!-- ==================== VERTICAL SPACER ======================= -->
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<TR>
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<TD class="WhiteCell NormalText"> </TD>
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</TR>
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</TABLE>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ======================== BOX10 =========================== -->
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<A NAME="VASTView"></A>
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
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<!-- ########## BLUE_HEADER ########## -->
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<TR>
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<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
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<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
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<B>How can I view or save a structure superposition?</B></TD>
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</TR>
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<!-- ########## END_BLUE_HEADER ########## -->
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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On a VAST results page (in either the "<A HREF="#VASTGraph">Graphics</A>" or the "<A HREF="#VASTTable">Table</A>" display), individual structure neighbors can be selected by clicking in the check boxes at the left margin. Then if one chooses the button labeled "View 3D Structure", the 3D superposition of the query protein with the selected neighbors is displayed in <a href="../CN3D/cn3d.shtml">Cn3D</a>. Up to 10 neighbors may be viewd in a superposition simultaneously, if Cn3D without the cache mechanism is selected (this is the default). This selection also works for Cn3D version 3.0.<BR><BR>
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Although the default is to submit all atoms for display in Cn3D, the "Backbone" option can be used to control the size of the files being downloaded by Cn3D, in order to save time and memory for data transmission to the viewer. With the release of Cn3D version 4.0, the Cn3D/Cache mechanism is used to store downloaded structure data locally. With this option, the number of neighbors for display is not limited. The user must take care not to exceed the physical memory available in his/her computer. If available memory is exceeded, Cn3D will not operate properly.<BR><BR>
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The <A HREF="../CN3D/cn3dtut.shtml">Cn3D Tutorial</A> provides additional details about <A HREF="../CN3D/cn3dtutP4.shtml">viewing structure alignments in Cn3D</A>.<BR><BR>
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Alternatively, instead of viewing the 3D superpositions, the data can be
|
|
examined or saved to disk as a local file, for browser-independent or later
|
|
viewing. Also if the "List" "Asn1" option is selected instead of the "List"
|
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"Graphics" or "List" "Table" from the last menu, a complete alignment file will be saved locally, including all of the neighbors in the subset.
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
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<img src="________.png" width="500" height="100"><BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR -->
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</TABLE>
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</TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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</TABLE>
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<!-- ======================== END_BOX10 =========================== -->
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<!-- ==================== VERTICAL SPACER ======================= -->
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<TR>
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<TD class="WhiteCell NormalText"> </TD>
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</TR>
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</TABLE>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ======================== BOX11 =========================== -->
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<A NAME="VASTHtml"></A>
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
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<!-- ########## BLUE_HEADER ########## -->
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<TR>
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<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
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|
<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
|
|
<B>How can I display a sequence alignment created from a structure superposition?</B></TD>
|
|
</TR>
|
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<!-- ########## END_BLUE_HEADER ########## -->
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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If the "View Alignment" button is chosen, a multiple alignment view will be opened in HTML, text, or FASTA with Gap formats. The check boxes at each neighbor "row" allow one to add the "Selected" neighbors into the alignments. The "All on page" option will allow a display of multiple alignments made from all of the neighbors on the same page.<BR><BR>
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|
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The HTML- and text-format alignment views indicate aligned vs. unaligned residues as uppercase and lowercase letters, respectively. In HTML views, columns with identical residues aligned across all selected sequences are colored red, whereas those with different aligned residues are colored blue. Those not covered by all sequences will be shown in grey.
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- TR>
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|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
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<img src="________.png" width="500" height="100"><BR><BR>
|
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</TD>
|
|
<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR -->
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</TABLE>
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</TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> 
|
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<BR> <BR></TD>
|
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</TR>
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</TABLE>
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|
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<!-- ======================== END_BOX11 =========================== -->
|
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<!-- ==================== VERTICAL SPACER ======================= -->
|
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|
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
|
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<TR>
|
|
<TD class="WhiteCell NormalText"> </TD>
|
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</TR>
|
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</TABLE>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ======================== BOX12 =========================== -->
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<A NAME="VASTNonbr"></A>
|
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll">
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<!-- ########## BLUE_HEADER ########## -->
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<TR>
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<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
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|
<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
|
|
<B>What does it mean when it says VAST did not find any structure neighbors?</B></TD>
|
|
</TR>
|
|
<!-- ########## END_BLUE_HEADER ########## -->
|
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<TR>
|
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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There are a few different reasons for this condition. One reason is simply that VAST does not consider this structure to be sufficiently similar to any other structure in the MMDB database. The VAST data use a statistical significance cutoff of P < 0.0001. This cutoff was set to be conservative intentionally, to reduce the number of false positives, but some hits that are biologically significant may be omitted because of this statistical threshold.<BR><BR>
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There are also some entries where the VAST calculation was not done: those for proteins with fewer than 3 secondary structure elements (SSEs), and structures containing no protein chains (i.e., only DNA or RNA). The molecule type and SSE count can be checked out by examining the structure with <a href="../CN3D/cn3d.shtml">Cn3D</a>.
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<!-- TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
|
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<img src="________.png" width="500" height="100"><BR><BR>
|
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
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</TR -->
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</TABLE>
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</TD>
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</TR>
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<TR>
|
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> 
|
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<BR> <BR></TD>
|
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</TR>
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</TABLE>
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<!-- ======================== END_BOX12 =========================== -->
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<!-- ==================== VERTICAL SPACER ======================= -->
|
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<TR>
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<TD class="WhiteCell NormalText"> </TD>
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</TR>
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</TABLE>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ======================== BOXN =========================== -->
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<A NAME="_____"></A>
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<!-- TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCellBlueEdgeAll" -->
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<!-- ########## BLUE_HEADER ########## -->
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<!-- TR>
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<TD class="SteelBlueCell HeaderText2B" ALIGN="LEFT" VALIGN="TOP">
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<A HREF="#Top"><img SRC="../IMG/arrowup_blue.gif" width="12" height="12" border="0" align="right" alt="back to top"></IMG></A>
|
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<B>_____________</B></TD>
|
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</TR -->
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<!-- ########## END_BLUE_HEADER ########## -->
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<!-- TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="LEFT" VALIGN="TOP">
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0" class="WhiteCell NormalText">
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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<TD class="WhiteCell NormalText" ALIGN="Left" VALIGN="TOP">
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Text text text text...<BR><BR>
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Text text text text...<BR><BR>
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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<TR>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
|
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP">
|
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<img src="________.png" width="500" height="100"><BR><BR>
|
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</TD>
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<TD class="WhiteCell" WIDTH="15" ALIGN="Center" VALIGN="TOP"> </TD>
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</TR>
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</TABLE>
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</TD>
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</TR>
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<TR>
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<TD class="WhiteCell NormalText" ALIGN="Center" VALIGN="TOP"> 
|
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<BR> <BR></TD>
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</TR>
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</TABLE -->
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<!-- ======================== END_BOXN =========================== -->
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</TD>
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<!-- ============== END_CONTENT_AREA_ROW_1_CELL_1 ================= -->
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</TR>
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</TABLE>
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<!-- ============== END_CONTENT_AREA_ROW_1 ================= -->
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<!-- ===== VERTICAL_SPACER_SEPARATING_ROW_1_and_ROW_2 ===== -->
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<!-- TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<TR>
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<TD class="WhiteCell NormalText"> </TD>
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</TR>
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</TABLE -->
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- ==================== VERTICAL SPACER ======================= -->
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<TABLE width="100%" border="0" cellspacing="0" cellpadding="0">
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<TR>
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<TD class="WhiteCell MicroText"> </TD>
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</TR>
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</TABLE>
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<!-- ================== END_VERTICAL SPACER ===================== -->
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<!-- end of content -->
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<!-- =========== PAGE_MARGIN_TO_RIGHT_OF_CONTENT_AREA =============== -->
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</TD>
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</TR>
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</TABLE>
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<!-- ############ END_BLUE_EDGE_BOX_WITH_TITLE_OF_DOCUMENT ######## -->
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<!-- =================== PAGE_FOOTER_AND_DATE_REVISED ================== -->
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<TABLE style="margin:0px 0px 0px 0px;" width="100%" border="0" cellspacing="0" cellpadding="0">
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<TR>
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<TD VALIGN="top" ALIGN="RIGHT"><SPAN CLASS="HELPBAR1" STYLE="color="#646464"">Revised 26 September 2016</SPAN></TD>
|
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<TD width="25"><IMG SRC="https://www.ncbi.nlm.nih.gov/coreweb/template1/pix/pixel.gif" width="1" height="1" alt="" border=0></TD>
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</TABLE>
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<!-- =================== END_PAGE_FOOTER_AND_DATE_REVISED ================== -->
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<!-- ##################### END_EDITABLE_CONTENT ##################### -->
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<script type="text/javascript">doc_tmp.getDocFtr('<A HREF="https://www.ncbi.nlm.nih.gov/research/">Computational Biology Branch</A> > <A HREF="https://www.ncbi.nlm.nih.gov/Structure/index.shtml">Structure Group</A> > <A HREF="https://www.ncbi.nlm.nih.gov/Structure/MMDB/mmdb.shtml">Macromolecular Structures</A> > <A HREF="https://www.ncbi.nlm.nih.gov/Structure/VAST/vast.shtml">VAST</A> > Help','other');</script>
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<!-- script type="text/javascript">doc_tmp.getDocFtr(' > <A HREF="______">__________</A> > <A HREF="______">__________</A> > <A HREF="______">__________</A> > ___currentpage_____', 'other');</script -->
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</body>
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</html>
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