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<article><div class="banner banner--gray banner--compact" style="--banner-background-image: url('/sites/default/files/media/image/2021-03/2015.jpg');--banner-background-focus: 50% 50%"><div class="banner__wrapper"><div class="banner__container"><h2 class="banner__heading">NIH Research Festival</h2><div class="banner__content"><p><strong><time datetime="2015-09-16">September 16</time> – <time datetime="2015-09-18">18, 2015</time></strong></p></div></div></div></div></article>
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<div class="section"><div data-drupal-messages-fallback class="hidden"></div></div><main role="main"><div id="main-content" tabindex="-1"></div><div class="section"><div class="content-sidebar"><div class="content-sidebar__main"><div class="content-sidebar__main-content"><div id="block-mainpagecontent-2"><article><h1>Improve prokaryotic RefSeq genome annotation based on Gpipe and manual curation</h1><div class="schedule"><div class="schedule__label"><div class="schedule__label-text"><time datetime="2015-09-18">Friday, September 18, 2015</time>
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– Poster Session IV
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</div></div><div class="schedule__content"><div class="schedule__item"><div class="schedule__time">12:00 – 1:30 p.m.</div><div class="schedule__description"><p><strong>FAES Terrace</strong></p></div><div class="schedule__description"><p><strong>NLM</strong></p></div><div class="schedule__description"><p><strong>GEN-28</strong></p></div></div></div></div><h2>Authors</h2><ul><li>W Li</li><li>K O'Neill</li><li>S Ciufo</li><li>K Pruitt</li></ul><h2>Abstract</h2><p>With the advancement of DNA sequencing technology, the number of sequenced bacterial genomes have rapidly increased in the last decade. However, these bacterial genomes have been annotated by different groups using distinct annotation programs, which causes a number of issues such as inconsistent protein names, uninformative annotation, and redundant proteins from clonal isolates. The National Center for Biotechnology Information (NCBI) RefSeq database is a collection of non-redundant and curated DNA, RNA, and protein sequences. NCBI has developed a prokaryotic genome annotation pipeline which is offered as a service for prokaryotic genome submissions to GenBank and is used to provide consistently annotated RefSeq prokaryotic genomes. A small dataset of 122 reference genomes that are manually annotated by collaborating groups and NCBI staff is also available. RefSeq has adopted a novel data-model for protein sequence representation that offers a significant reduction in protein redundancy and has additional benefits in terms of managing and updating prokaryotic protein names. RefSeq prokaryotic genome data can be accessed in BLAST databases, web resources (Assembly, BioProject, Genome, Nucleotide, and Protein), can be accessed using NCBI’s programming utilities, or can be downloaded from the genomes or refseq FTP sites.</p><p><strong>Scientific Focus Area</strong>: Genetics and Genomics</p><p class="updated"><small>This page was last updated on Friday, March 26, 2021</small></p></article>
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