BankIt Submission tool requires the submitter to identify the forward and reverse primers used to determine the sequences of the set.
Primers may be identified by their commercial names, their sequences, or both.
Primer sequences should be presented in 5' > 3' order. The sequences should be given in the IUPAC degenerate-base alphabet, except for the modified (extended) bases; those must be enclosed within angle brackets <>. For example ACACAG<i>GTACAC.
When more than one forward and/or reverse primer reactions are performed to
determine a sequence, separate the sequences of each primer reaction with
commas and enclose all of them in parentheses. For example,
(GACGTTGTAAAACGACGGCC, TGTAAAACGACGGCCAGT, TATGGGTCCAGT).
When more than one forward and/or reverse primer is used in a single reaction
to determine a sequence,
separate the sequences of each primer with colons. For example,
ACGTGACGTTGTAAAACGACGGCC:ACACTGTAAAACGACGGCCAGT:TATGGGTCCAGTTTT.
If the sequences in a set were determined using different primers, the primers used to determine the largest number of the sequences can be identified in the Primers - 'Set One Value' section of the submission tool and alternate primers can be identified in the primers table. Table column headers to list alternate primers are shown below.
| Primer | Column Header |
| Forward primer name | fwd_primer_name |
| Forward primer sequence | fwd_primer_seq |
| Reverse primer name | rev_primer_name |
| Reverse primer sequence | rev_primer_seq |
When different primers are used for determining
sequences in a BankIt Set, enter the set of forward and reverse primers
most frequently used in the Primers - 'Set One Value' section of
the submission tool. Then use the primers table to indicate
alternate primer sequences. The BankIt submission tool will use the
primer values from the Primers page for any blanks in a primer column in
the primers table. A sample primers table is show below.
Note:
| Sequence_ID | fwd_primer_seq | rev_primer_seq | fwd_primer_name | rev_primer_name |
| Seq1 | GACGTTGTAAAACGACGGCC | CACAGGAAACAGCTATGACC | COX_forward | COX_reverse |
| Seq2 | TGTAAAACGACGGCCAGT | CAGGAAACAGCTATGACC | COX_forward | COX_reverse |
| Seq3 | COX_forward | COX_reverse | ||
| Seq4 | TAATACGACTCACTATAGGG:TGTAAACACACACACAGGTT | ATTTAGGTGACACTATAG:CAGTGACACACACGTACA | COX_forward | COX_reverse |
| Seq5 | COX_forward | COX_reverse | ||
| Seq6 | GACGTTGTAAAACGACGGCC | (CAGGAAACAGCTATGACC,TGTAAAACGACGGCCAGT) | COX_forward | COX_reverse |
| Seq7 | (CAGGAAACAGCTATGACC:ATTTAGGTGACACTATAG,GACGTTGTAACGACCGGAA:TTTTTCACAGTACGTTACT) | TGTAAAACGACGGCCAGT | COX_forward | COX_reverse |