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. 1981:370:812-21.
doi: 10.1111/j.1749-6632.1981.tb29787.x.

Chromogenic substrate spectrophotometric assays for the measurement of clotting function

P A Aiyappa

Chromogenic substrate spectrophotometric assays for the measurement of clotting function

P A Aiyappa. Ann N Y Acad Sci. 1981.

Abstract

Spectrophotometric assays for the measurement of coagulation factor activities in plasma have been developed using a relatively specific chromogenic substrate for thrombin. The tissue-factor-activated assay system involves the activation of factors VII and X and prothrombin. The surface-activated assay system involves the activation of factors XII, PK, XI, IX, VII, X and prothrombin using factors V and VIII as cofactors. The thrombin generated subsequent to activation in these two assay systems is measured via its activity on the chromogenic substrate Sarc-Pro-Arg-pNA. These spectrophotometric assays provide a higher degree of sensitivity as compared with the clotting time assays. The tissue-factor-activated system provides a sensitive method for monitoring coumarin therapy. The surface-activated assay system can detect deficiencies of all the factors measured by the conventional APTT assay system, as well as factor VII. Furthermore, this assay provides a sensitive method for monitoring heparin therapy.

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