doi: 10.1128/jb.162.1.92-97.1985.
Transformation and fusion of Streptococcus faecalis protoplasts
- PMID: 3920209
- PMCID: PMC218958
- DOI: 10.1128/jb.162.1.92-97.1985
Item in Clipboard
Transformation and fusion of Streptococcus faecalis protoplasts
J Bacteriol.
1985 Apr.
Abstract
Nonconjugative plasmids were transferred by protoplast fusion among Streptococcus faecalis strains and from Streptococcus sanguis to S. faecalis. S. faecalis protoplasts were also transformed with several different plasmids, including the Tn917 delivery vehicle pTV1. Transformation was reproducible, but low in frequency (10(-6) transformants per viable protoplast). A new shuttle vector (pAM610), able to replicate in Escherichia coli and S. faecalis, was constructed and transformed into S. faecalis protoplasts. pAM610 was mobilized by the conjugative plasmid pAM beta 1 in matings among S. faecalis strains and from S. sanguis to S. faecalis. Chimeric derivatives of pAM610 were also transformed into S. faecalis.
Similar articles
-
Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.J Bacteriol. 1986 Mar;165(3):831-6. doi: 10.1128/jb.165.3.831-836.1986. J Bacteriol. 1986. PMID: 3005240 Free PMC article.
-
Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.J Bacteriol. 1984 Dec;160(3):1109-14. doi: 10.1128/jb.160.3.1109-1114.1984. J Bacteriol. 1984. PMID: 6094500 Free PMC article.
-
Conjugal plasmid transfer (pAM beta 1) in Lactobacillus plantarum.Appl Environ Microbiol. 1986 Sep;52(3):574-6. doi: 10.1128/aem.52.3.574-576.1986. Appl Environ Microbiol. 1986. PMID: 3094451 Free PMC article.
-
Generation of Tn5 insertions in streptococcal conjugative transposon Tn916.Appl Environ Microbiol. 1987 May;53(5):1069-72. doi: 10.1128/aem.53.5.1069-1072.1987. Appl Environ Microbiol. 1987. PMID: 3038014 Free PMC article.
-
The sex pheromone system of Enterococcus faecalis. More than just a plasmid-collection mechanism?Eur J Biochem. 1994 Jun 1;222(2):235-46. doi: 10.1111/j.1432-1033.1994.tb18862.x. Eur J Biochem. 1994. PMID: 8020463 Review.
Cited by
-
Two conjugation systems associated with Streptococcus faecalis plasmid pCF10: identification of a conjugative transposon that transfers between S. faecalis and Bacillus subtilis.J Bacteriol. 1987 Jun;169(6):2529-36. doi: 10.1128/jb.169.6.2529-2536.1987. J Bacteriol. 1987. PMID: 3034859 Free PMC article.
-
Intergeneric Protoplast Fusion between Ruminococcus albus and an Anaerobic Recombinant, FE7.Appl Environ Microbiol. 1988 May;54(5):1249-53. doi: 10.1128/aem.54.5.1249-1253.1988. Appl Environ Microbiol. 1988. PMID: 16347634 Free PMC article.
-
Protoplast formation and regeneration of dehydrodivanillin-degrading strains of Fusobacterium varium and Enterococcus faecium.Appl Environ Microbiol. 1986 Oct;52(4):612-6. doi: 10.1128/aem.52.4.612-616.1986. Appl Environ Microbiol. 1986. PMID: 3777921 Free PMC article.
-
Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.J Bacteriol. 1986 Mar;165(3):831-6. doi: 10.1128/jb.165.3.831-836.1986. J Bacteriol. 1986. PMID: 3005240 Free PMC article.
-
Cloning in Streptococcus lactis of plasmid-mediated UV resistance and effect on prophage stability.Appl Environ Microbiol. 1986 Feb;51(2):233-7. doi: 10.1128/aem.51.2.233-237.1986. Appl Environ Microbiol. 1986. PMID: 3006588 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous
