Ultrasound assisted extraction and quantification of targeted bioactive compounds of Centella asiatica (Gotu Kola) by UHPLC-MS/MS MRM tandem mass spectroscopy

doi:10.1016/j.foodchem.2021.131187

. 2022 Mar 1:371:131187.

doi: 10.1016/j.foodchem.2021.131187. Epub 2021 Sep 20.

Ultrasound assisted extraction and quantification of targeted bioactive compounds of Centella asiatica (Gotu Kola) by UHPLC-MS/MS MRM tandem mass spectroscopy

Rasangani Sabaragamuwa¹, Conrad O Perera², Bruno Fedrizzi³

Affiliations

¹ Food Science Programme, School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Department of Food Science and Technology, Faculty of Applied Sciences, Sabaragamuwa University of Sri Lanka, Belihuloya, Sri Lanka.
² Food Science Programme, School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address: c.perera@auckland.ac.nz.
³ Food Science Programme, School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.

PMID: 34592625
DOI: 10.1016/j.foodchem.2021.131187

Ultrasound assisted extraction and quantification of targeted bioactive compounds of Centella asiatica (Gotu Kola) by UHPLC-MS/MS MRM tandem mass spectroscopy

Rasangani Sabaragamuwa et al. Food Chem. 2022.

. 2022 Mar 1:371:131187.

doi: 10.1016/j.foodchem.2021.131187. Epub 2021 Sep 20.

Authors

Rasangani Sabaragamuwa¹, Conrad O Perera², Bruno Fedrizzi³

Affiliations

¹ Food Science Programme, School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Department of Food Science and Technology, Faculty of Applied Sciences, Sabaragamuwa University of Sri Lanka, Belihuloya, Sri Lanka.
² Food Science Programme, School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address: c.perera@auckland.ac.nz.
³ Food Science Programme, School of Chemical Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.

PMID: 34592625
DOI: 10.1016/j.foodchem.2021.131187

Abstract

Centella asiatica (Gotu Kola) is a green leafy vegetable rich in phytochemicals mainly including triterpenes and caffeoylquinic acids. Fresh leaves of this plant are consumed in salads and beverages in a variety of cuisines around the world. This is a well-known functional food for its neuroprotective and cognition enhancing properties in traditional societies. HPLC-DAD at lower wavelengths commonly used to identify and quantify major triterpenes of C. asiatica extracts, but associated with few drawbacks. This paper discusses a specific, sensitive and validated method developed based on UHPLC-ESI-MS-MS-MRM tandem mass spectroscopy for targeted quantification of C. asiatica bioactive compounds. The validated method enabled a precise estimation of major triterpenes and chlorogenic acid in C. asiatica in a shorter time. The findings of this study will contribute to the information on chemotype variation of C. asiatica plant grown under unique geographical, environmental and climatic conditions in New Zealand.

Keywords: Centella asiatica; Chlorogenic acid; Mass spectroscopy; Neuroprotective; Targeted quantification; Triterpenes.

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Ultrasound assisted extraction and quantification of targeted bioactive compounds of Centella asiatica (Gotu Kola) by UHPLC-MS/MS MRM tandem mass spectroscopy

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Ultrasound assisted extraction and quantification of targeted bioactive compounds of Centella asiatica (Gotu Kola) by UHPLC-MS/MS MRM tandem mass spectroscopy

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