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. 2018 Feb;124(2):457-468.
doi: 10.1111/jam.13642.

Efficient production of tri-acetylated mono-acylated mannosylerythritol lipids by Sporisorium sp. aff. sorghi SAM20

N Alimadadi  1 , M R Soudi  1 , Z Talebpour  2
Affiliations

Efficient production of tri-acetylated mono-acylated mannosylerythritol lipids by Sporisorium sp. aff. sorghi SAM20

N Alimadadi et al. J Appl Microbiol. 2018 Feb.

Abstract

Aims: The aim of this study was to isolate a novel yeast strain, evaluate biosurfactant production by the strain and characterize the major product.

Methods and results: The strain SAM20, isolated from grass, identified as Sporisorium sp. aff. sorghi based on phylogenetic analyses. The strain produced approximately 32 g l-1 glycolipid biosurfactants from 40 g l-1 soybean oil after 7 days at 28°C. The glycolipids showed a unique pattern of mannosylerythritol lipids (MELs) on thin layer chromatography plate compared to those hitherto reported. Structural characterization of the major product, called GL-A, revealed that it was mainly tri-acetylated mono-acylated MELs (MEL-A2) with C14:0, C16:0, C12:0 or C14:1 as the hydrophobic chain. The critical micelle concentration (CMC), the surface tension at CMC and hydrophilic-lipophilic balance value for GL-A were estimated to be 20 mg l-1 , 30·0 mN m-1 and 8·7, respectively.

Conclusions: A MEL-A2 with novel composition and surface activities was efficiently produced from a novel MEL producer. This is the first report on production of MEL-A2 as the major product and from soybean oil. The biosurfactant has potential application as a wetting agent and oil-in-water emulsifier.

Significance and impact of the study: Discovery of novel structures and novel strains is valuable for further commercial development and application of MELs. Sporisorium sp. aff. sorghi SAM20 can be considered as a potential candidate for commercial production of biosurfactants.

Keywords: Sporisorium sp. aff. sorghi; biosurfactant; glycolipid; hydrophilic-lipophilic balance; mannosylerythritol lipid; surface activity.

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