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. 2004 Jun;12(6):949-59.
doi: 10.1016/j.str.2004.03.026.

The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea

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The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea

Hiroshi Nishimasu et al. Structure. 2004 Jun.
Free article

Abstract

As the first structure of the novel class of fructose-1,6-bisphosphatase (FBPase) present in thermophilic archaea, we solved the crystal structure of the ST0318 gene product (St-Fbp) of Sulfolobus tokodaii strain 7. The St-Fbp structure comprises a homooctamer of the 422 point-group. The protein folds as a four-layer alpha-beta-beta-alpha sandwich with a novel topology, which is completely different from the sugar phosphatase fold. The structure contains an unhydrolyzed FBP molecule in the open-keto form, as well as four hexacoordinated magnesium ions around the 1-phosphoryl group of FBP. The arrangement of the catalytic side chains and metal ligands is consistent with the three-metal ion assisted catalysis proposed for conventional FBPases. The structure provides an insight into the structural basis of the strict substrate specificity of St-Fbp.

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