Eyes absent represents a class of protein tyrosine phosphatases
- PMID: 14628052
- DOI: 10.1038/nature02093
Eyes absent represents a class of protein tyrosine phosphatases
Abstract
The Eyes absent proteins are members of a conserved regulatory network implicated in the development of the eye, muscle, kidney and ear. Mutations in the Eyes absent genes have been associated with several congenital disorders including the multi-organ disease bronchio-oto-renal syndrome, congenital cataracts and late-onset deafness. On the basis of previous analyses it has been shown that Eyes absent is a nuclear transcription factor, acting through interaction with homeodomain-containing Sine oculis (also known as Six) proteins. Here we show that Eyes absent is also a protein tyrosine phosphatase. It does not resemble the classical tyrosine phosphatases that use cysteine as a nucleophile and proceed by means of a thiol-phosphate intermediate. Rather, Eyes absent is the prototype for a class of protein tyrosine phosphatases that use a nucleophilic aspartic acid in a metal-dependent reaction. Furthermore, the phosphatase activity of Eyes absent contributes to its ability to induce eye formation in Drosophila.
Comment in
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Signal transduction: an eye on organ development.Nature. 2003 Nov 20;426(6964):238-9. doi: 10.1038/426238a. Nature. 2003. PMID: 14628032 No abstract available.
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