Purification and characterization of aspartate racemase from the bivalve mollusk Scapharca broughtonii
- PMID: 12568809
- DOI: 10.1016/s1096-4959(02)00267-1
Purification and characterization of aspartate racemase from the bivalve mollusk Scapharca broughtonii
Abstract
High concentrations of D-aspartate occur in blood shell Scapharca broughtonii (Mollusca) tissues. We purified aspartate racemase from the foot muscle of the bivalve to electrophoretic homogeneity. The molecular mass shown by sodium dodecyl sulfate polyacrylamide gel was 39 kDa, while that shown by gel filtration ranged from 51 to 63 kDa. Pyridoxal 5'-phosphate-dependency of the enzyme was demonstrated by its absorption spectrum as well as the effects of amino-oxyacetate and other reagents on the activity and spectrum. The enzyme is highly specific to aspartate and does not racemize L-alanine, L-serine and L-glutamate. It showed the highest activity at pH 8 both in the conversion of L- to D- and D- to L-aspartate, and the optimal temperature was 25 degrees C. V(max) and K(m) values for L-aspartate were 7.39 micromolmin(-1)mg(-1) and 60.4 mM and those for D-aspartate were 22.6 micromolmin(-1)mg(-1) and 159 mM, respectively.
Similar articles
-
Crystal structure of a pyridoxal 5'-phosphate-dependent aspartate racemase derived from the bivalve mollusc Scapharca broughtonii.Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):651-656. doi: 10.1107/S2053230X17015813. Epub 2017 Nov 6. Acta Crystallogr F Struct Biol Commun. 2017. PMID: 29199985 Free PMC article.
-
Molecular cloning and enzymological characterization of pyridoxal 5'-phosphate independent aspartate racemase from hyperthermophilic archaeon Thermococcus litoralis DSM 5473.Extremophiles. 2016 Sep;20(5):711-21. doi: 10.1007/s00792-016-0860-8. Epub 2016 Jul 20. Extremophiles. 2016. PMID: 27438592
-
Cloning and expression of the pyridoxal 5'-phosphate-dependent aspartate racemase gene from the bivalve mollusk Scapharca broughtonii and characterization of the recombinant enzyme.J Biochem. 2006 Feb;139(2):235-44. doi: 10.1093/jb/mvj028. J Biochem. 2006. PMID: 16452311
-
Molecular identification of monomeric aspartate racemase from Bifidobacterium bifidum.Eur J Biochem. 2004 Dec;271(23-24):4798-803. doi: 10.1111/j.1432-1033.2004.04445.x. Eur J Biochem. 2004. PMID: 15606767
-
Purification and properties of ornithine racemase from Clostridium sticklandii.J Bacteriol. 2000 Apr;182(7):2052-4. doi: 10.1128/JB.182.7.2052-2054.2000. J Bacteriol. 2000. PMID: 10715017 Free PMC article.
Cited by
-
Crystal structure of a pyridoxal 5'-phosphate-dependent aspartate racemase derived from the bivalve mollusc Scapharca broughtonii.Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):651-656. doi: 10.1107/S2053230X17015813. Epub 2017 Nov 6. Acta Crystallogr F Struct Biol Commun. 2017. PMID: 29199985 Free PMC article.
-
The role and molecular mechanism of D-aspartic acid in the release and synthesis of LH and testosterone in humans and rats.Reprod Biol Endocrinol. 2009 Oct 27;7:120. doi: 10.1186/1477-7827-7-120. Reprod Biol Endocrinol. 2009. PMID: 19860889 Free PMC article. Clinical Trial.
-
A new in vitro anti-tumor polypeptide isolated from Arca inflata.Mar Drugs. 2013 Dec 2;11(12):4773-87. doi: 10.3390/md11124773. Mar Drugs. 2013. PMID: 24317469 Free PMC article.
-
A novel series of metazoan L/D peptide isomerases.J Biol Chem. 2024 Jul;300(7):107458. doi: 10.1016/j.jbc.2024.107458. Epub 2024 Jun 8. J Biol Chem. 2024. PMID: 38857862 Free PMC article.
-
Molecular cloning and enzymological characterization of pyridoxal 5'-phosphate independent aspartate racemase from hyperthermophilic archaeon Thermococcus litoralis DSM 5473.Extremophiles. 2016 Sep;20(5):711-21. doi: 10.1007/s00792-016-0860-8. Epub 2016 Jul 20. Extremophiles. 2016. PMID: 27438592
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
