HGNC Approved Gene Symbol: TRIM11
Cytogenetic location: 1q42.13 Genomic coordinates (GRCh38) : 1:228,393,676-228,406,835 (from NCBI)
Using a B-box domain consensus sequence to screen EST databases for novel TRIM family members, Reymond et al. (2001) identified and cloned mouse and human TRIM11. The deduced protein contains an N-terminal RING domain, a type-2 B-box domain, a coiled-coil region, and 3 C-terminal RFP (B30.2) (602165)-like domains. Northern blot analysis of adult human tissues indicated ubiquitous expression, and in situ hybridization of mouse embryos showed expression in CNS, thymus, kidney, and gut. Fluorescence-tagged TRIM11 showed diffuse nuclear and cytoplasmic expression in transfected osteocarcinoma and HeLa cells. Disruption of the RING domain, the B-box domain, or the coiled-coil region caused redistribution and more diffuse staining of cytoplasmic and nuclear structures.
Using several in vitro and in vivo protein-protein interaction techniques, Reymond et al. (2001) found that TRIM11 forms high molecular mass homomultimer complexes. Mutation analysis of other TRIM proteins suggested that the coiled-coil domain mediates complex formation.
By means of a yeast 2-hybrid screening assay, Niikura et al. (2003) found that TRIM11 interacts with humanin (HN; 561010), a peptide with neuroprotective activities against Alzheimer disease (104300)-associated insults. Both the coiled-coil domain and the C-terminal RFP-like domain of TRIM11 were necessary for interaction with HN. Coexpression of HN and TRIM11 diminished intracellular levels of HN. The authors noted that the TRIM11 RING finger domain acts as a putative E3 ubiquitin ligase, and suggested that TRIM11 regulates intracellular HN levels through ubiquitin-mediated protein degradation pathways.
By radiation hybrid analysis, Reymond et al. (2001) mapped the TRIM11 gene to chromosome 1q32-q44.
Niikura, T., Hashimoto, Y., Tajima, H., Ishizaka, M., Yamagishi, Y., Kawasumi, M., Nawa, M., Terashita, K., Aiso, S., Nishimoto, I. A tripartite motif protein TRIM11 binds and destabilizes humanin, a neuroprotective peptide against Alzheimer's disease-relevant insults. Europ. J. Neurosci. 17: 1150-1158, 2003. [PubMed: 12670303] [Full Text: https://doi.org/10.1046/j.1460-9568.2003.02553.x]
Reymond, A., Meroni, G., Fantozzi, A., Merla, G., Cairo, S., Luzi, L., Riganelli, D., Zanaria, E., Messali, S., Cainarca, S., Guffanti, A., Minucci, S., Pelicci, P. G., Ballabio, A. The tripartite motif family identifies cell compartments. EMBO J. 20: 2140-2151, 2001. [PubMed: 11331580] [Full Text: https://doi.org/10.1093/emboj/20.9.2140]