Box 1, Example Calculation to Determine Nanoparticle Concentration for In Vitro Tests - National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols

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Box 1Example Calculation to Determine Nanoparticle Concentration for In Vitro Tests

In this example, we assume a mouse dose of 123 mg/kg. Therefore, the scaled equivalent human dose would be:

H u m a n d o s e = \frac{m o u s e d o s e}{12.3} = \frac{123 m g / k g}{12.3} = 10 m g / k g

Blood volume constitutes approximately 8% of the body weight. Therefore, an average human of 70 kg body weight has approximately 5.6 L of blood. Assuming all the nanoparticle injected goes into the systemic circulation, this provides a rough approximation of the potential maximum nanoparticle concentration in blood, which is used as the in vitro test concentration.

i n v i t r o c o n c e n t r a t i o n_{h u m a n m a t r i x} = \frac{h u m a n d o s e}{h u m a n b l o o d v o l u m e} = \frac{70 k g * 10 m g / k g}{5.6 L} = 0.125 m g / m L

From: NCL Method ITA-14, Analysis of Nanoparticle Effects on Maturation of Monocyte Derived Dendritic Cells In Vitro

Cover of National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols

National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet].

Bethesda (MD): National Cancer Institute (US); 2005 May 1-.

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