9AR4: CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM

PDB ID: 9AR4Download
MMDB ID: 259972
PDB Deposition Date: 2024/2/22
Updated in MMDB: 2025/03
Experimental Method:
electron microscopy
Resolution: 2.2  Å
Source Organism:
Geobacillus thermodenitrificans▼
Similar Structures:
Biological Unit for 9AR4: hexameric; determined by author
Molecular Components in 9AR4
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cas9
Molecule annotation
Nucleotides(5 molecules)
1
Single Guide RNA
Molecule annotation
1
Cleaved 3' Target DNA Strand
Molecule annotation
1
Cleaved 3' Non-target DNA Strand
Molecule annotation
1
Cleaved 5' Target DNA Strand
Molecule annotation
1
Cleaved 5' Non-target DNA Strand
Molecule annotation
Chemicals and Non-standard biopolymers (7 molecules)
1
6
MAGNESIUM IONMAGNESIUM ION
2
1
GUANOSINE-5'-TRIPHOSPHATEGUANOSINE-5'-TRIPHOSPHATE
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303