Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K
- PMID: 728998
- DOI: 10.1016/0092-8674(78)90046-6
Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K
Abstract
A non-self-replicating segment (1370 base pairs) of plasmid R6K was cloned in E. coli and shown to trans-complement temperature-sensitive replication mutants of this plasmid. This segment contains the gene which codes for a protein required for initiation of replication of the plasmid, and was used as a helper in a functional assay for an origin of replication in R6K derivatives. A 420 bp fragment, derived from R6K DNA, was shown to carry a functional origin since it was capable of replicating as a plasmid in E. coli cells carrying the helper segment either on the host chromosome or on a plasmid Col E1 derivative. The copy number of the origin fragment in cells carrying the helper segment on the chromosome is essentially the same as the copy number of R6K. A model for the positive regulation of plasmid R6K replication is presented.
Similar articles
-
Preponderance of Fis-binding sites in the R6K gamma origin and the curious effect of the penicillin resistance marker on replication of this origin in the absence of Fis.J Bacteriol. 1996 Aug;178(16):4965-74. doi: 10.1128/jb.178.16.4965-4974.1996. J Bacteriol. 1996. PMID: 8759862 Free PMC article.
-
Binding of purified wild-type and mutant pi initiation proteins to a replication origin region of plasmid R6K.J Mol Biol. 1986 Jan 20;187(2):225-39. doi: 10.1016/0022-2836(86)90230-5. J Mol Biol. 1986. PMID: 3009827
-
Identification and characterization of the functional alpha origin of DNA replication of the R6K plasmid and its relatedness to the R6K beta and gamma origins.Mol Gen Genet. 1987 Jun;208(1-2):263-70. doi: 10.1007/BF00330452. Mol Gen Genet. 1987. PMID: 3302610
-
Binding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi protein.J Bacteriol. 1994 Nov;176(22):6795-801. doi: 10.1128/jb.176.22.6795-6801.1994. J Bacteriol. 1994. PMID: 7961437 Free PMC article.
-
Replication properties of plasmic R6K.Plasmid. 1981 Jan;5(1):2-9. doi: 10.1016/0147-619x(81)90073-1. Plasmid. 1981. PMID: 6261282 Review. No abstract available.
Cited by
-
Colonization strategies of Pseudomonas fluorescens Pf0-1: activation of soil-specific genes important for diverse and specific environments.BMC Microbiol. 2013 Apr 27;13:92. doi: 10.1186/1471-2180-13-92. BMC Microbiol. 2013. PMID: 23622502 Free PMC article.
-
Replication initiator protein of plasmid R6K autoregulates its own synthesis at the transcriptional step.Proc Natl Acad Sci U S A. 1985 May;82(9):2574-8. doi: 10.1073/pnas.82.9.2574. Proc Natl Acad Sci U S A. 1985. PMID: 3857600 Free PMC article.
-
Transformation and fusion of Streptococcus faecalis protoplasts.J Bacteriol. 1985 Apr;162(1):92-7. doi: 10.1128/jb.162.1.92-97.1985. J Bacteriol. 1985. PMID: 3920209 Free PMC article.
-
Nucleotide sequence of the region of an origin of replication of the antibiotic resistance plasmid R6K.Proc Natl Acad Sci U S A. 1979 Mar;76(3):1150-4. doi: 10.1073/pnas.76.3.1150. Proc Natl Acad Sci U S A. 1979. PMID: 375227 Free PMC article.
-
cis and trans factors affecting Mos1 mariner evolution and transposition in vitro, and its potential for functional genomics.Nucleic Acids Res. 2000 Feb 1;28(3):784-90. doi: 10.1093/nar/28.3.784. Nucleic Acids Res. 2000. PMID: 10637331 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
