Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review

Analysis of Hemolytic Properties of Nanoparticles: Version 4

Barry W. Neun  1 , Edward Cedrone  1 , Marina A. Dobrovolskaia  1
In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May. NCL Method ITA-1.
2020 May.
Affiliations
Free Books & Documents
Review

Analysis of Hemolytic Properties of Nanoparticles: Version 4

Barry W. Neun et al.
Free Books & Documents

Excerpt

Erythrocytes comprise approximately 45% of whole blood by volume. Hemolysis refers to the damage of red blood cells leading to the release of erythrocyte intracellular content into blood plasma. When it occurs, in vivo hemolysis can lead to anemia, jaundice and other pathological conditions, which may become life threatening. Hemoglobin is a dominant protein carried by erythrocytes. When it is contained inside the cell it plays a key role in carrying oxygen to other cells and tissues. However, extracellular hemoglobin is toxic and may affect vascular, myocardial, renal and central nervous system functions. Therefore, all medical devices and drugs which come in contact with blood are required to be tested for potential hemolytic properties.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Malinauskas R.A. Plasma hemoglobin measurement techniques for the in vitro evaluation of blood damage called by medical devices. Artif. Organs 1997, 21:1255–1267 - PubMed
    1. ASTM standard practice F 756-00. Assessment of hemolytic properties of materials.
    1. ASTM E2524-08 (2013) Standard Test Method for Analysis of Hemolytic Properties of Nanoparticles
    1. Dobrovolskaia MA, McNeil SE. Understanding the correlation between in vitro and in vivo immunotoxicity tests for nanomedicines. J Control Release. 2013. Dec 10;172(2):456–66 - PMC - PubMed
    1. CDER, Guidance for Industry: Estimating the Maximum Safe Starting Dose in Initial Clinical Trials for Therapeutics in Adult Healthy Volunteers, 2005.

LinkOut - more resources