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Review
. 2017 Sep;18(9):517-534.
doi: 10.1038/nrg.2017.33. Epub 2017 May 30.

TET-mediated active DNA demethylation: mechanism, function and beyond

Xiaoji Wu  1   2   3   4   5 , Yi Zhang  1   2   3   4
Affiliations
Review

TET-mediated active DNA demethylation: mechanism, function and beyond

Xiaoji Wu et al. Nat Rev Genet. 2017 Sep.

Abstract

In mammals, DNA methylation in the form of 5-methylcytosine (5mC) can be actively reversed to unmodified cytosine (C) through TET dioxygenase-mediated oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), followed by replication-dependent dilution or thymine DNA glycosylase (TDG)-dependent base excision repair. In the past few years, biochemical and structural studies have revealed mechanistic insights into how TET and TDG mediate active DNA demethylation. Additionally, many regulatory mechanisms of this process have been identified. Technological advances in mapping and tracing the oxidized forms of 5mC allow further dissection of their functions. Furthermore, the biological functions of active DNA demethylation in various biological contexts have also been revealed. In this Review, we summarize the recent advances and highlight key unanswered questions.

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References

    1. J Exp Med. 2016 Mar 7;213(3):377-97 - PubMed
    1. J Biol Chem. 2014 Feb 28;289(9):5986-96 - PubMed
    1. EMBO Rep. 2014 Dec;15(12):1305-14 - PubMed
    1. Nature. 2013 Aug 8;500(7461):222-6 - PubMed
    1. Nature. 2011 Feb 17;470(7334):419-23 - PubMed

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