"Nat Protoc"[jour] - PMC

Sidharth Tyagi, Grant P. Higerd-Rusli, Elizabeth J. Akin, Christopher A. Baker, Shujun Liu, Fadia B. Dib-Hajj, Stephen G. Waxman, Sulayman D. Dib-Hajj

Nat Protoc. Author manuscript; available in PMC 2025 Mar 1.

Published in final edited form as: Nat Protoc. 2024 Sep; 19(9): 2771–2802. Published online 2024 Jun 3. doi: 10.1038/s41596-024-00997-x

PMCID:: PMC11721981

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Select item 113805945.

Generation of ‘semi-guided’ cortical organoids with complex neural oscillations

Michael Q. Fitzgerald, Tiffany Chu, Francesca Puppo, Rebeca Blanch, Miguel Chillón, Shankar Subramaniam, Alysson R. Muotri

Nat Protoc. Author manuscript; available in PMC 2024 Sep 8.

Published in final edited form as: Nat Protoc. 2024 Sep; 19(9): 2712–2738. Published online 2024 May 3. doi: 10.1038/s41596-024-00994-0

Correction in:: Nat Protoc. 2024 Oct 15

PMCID:: PMC11380594

Article PDF–2.8M

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Control of DNA replication in vitro using a reversible replication barrier

Emma J Vontalge, Tamar Kavlashvili, Steven N Dahmen, Matthew T Cranford, James M Dewar

Nat Protoc. Author manuscript; available in PMC 2024 Jul 9.

Published in final edited form as: Nat Protoc. 2024 Jul; 19(7): 1940–1983. Published online 2024 Apr 9. doi: 10.1038/s41596-024-00977-1

PMCID:: PMC11230854

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Anaerobic cryoEM protocols for air-sensitive nitrogenase proteins

Rebeccah A. Warmack, Belinda B. Wenke, Thomas Spatzal, Douglas C. Rees

Nat Protoc. Author manuscript; available in PMC 2024 Nov 1.

Published in final edited form as: Nat Protoc. 2024 Jul; 19(7): 2026–2051. Published online 2024 Apr 4. doi: 10.1038/s41596-024-00973-5

PMCID:: PMC11528890

Article PDF–2.9M

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Engineering megabase-sized genomic deletions with MACHETE (Molecular Alteration of Chromosomes with Engineered Tandem Elements)

Francisco M. Barriga, Scott W. Lowe

Nat Protoc. Author manuscript; available in PMC 2024 Dec 13.

Published in final edited form as: Nat Protoc. 2024 May; 19(5): 1381–1399. Published online 2024 Feb 7. doi: 10.1038/s41596-024-00953-9

PMCID:: PMC11642290

Article PDF–1.4M

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Bacterial genome engineering using CRISPR-associated transposases

Diego Rivera Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris H. Wang, Samuel H. Sternberg

Nat Protoc. Author manuscript; available in PMC 2025 Jan 6.

Published in final edited form as: Nat Protoc. 2024 Mar; 19(3): 752–790. Published online 2024 Jan 12. doi: 10.1038/s41596-023-00927-3

PMCID:: PMC11702153

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Analyzing somatic mutations by single-cell whole-genome sequencing

Lei Zhang, Moonsook Lee, Alexander Y. Maslov, Cristina Montagna, Jan Vijg, Xiao Dong

Nat Protoc. Author manuscript; available in PMC 2024 Sep 17.

Published in final edited form as: Nat Protoc. 2024 Feb; 19(2): 487–516. Published online 2023 Nov 23. doi: 10.1038/s41596-023-00914-8

PMCID:: PMC11406548

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Scalable single-cell profiling of chromatin modifications with sciCUT&Tag

Derek H. Janssens, Jacob E. Greene, Steven J. Wu, Christine A. Codomo, Sam S. Minot, Scott N. Furlan, Kami Ahmad, Steven Henikoff

Nat Protoc. Author manuscript; available in PMC 2024 Jul 8.

Published in final edited form as: Nat Protoc. 2024 Jan; 19(1): 83–112. Published online 2023 Nov 7. doi: 10.1038/s41596-023-00905-9

PMCID:: PMC11229882

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Guide to the construction and use of an adaptive optics two-photon microscope with direct wavefront sensing

Pantong Yao, Rui Liu, Thomas Broginni, Martin Thunemann, David Kleinfeld

Nat Protoc. Author manuscript; available in PMC 2024 Dec 1.

Published in final edited form as: Nat Protoc. 2023 Dec; 18(12): 3732–3766. Published online 2023 Nov 1. doi: 10.1038/s41596-023-00893-w

PMCID:: PMC11033548

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Precise surface functionalization of PLGA particles for human T cell modulation

Pierce Hadley, Yuanzhou Chen, Lariana Cline, Zhiyuan Han, Qizhi Tang, Xiao Huang, Tejal Desai

Nat Protoc. Author manuscript; available in PMC 2024 Jan 9.

Published in final edited form as: Nat Protoc. 2023 Nov; 18(11): 3289–3321. Published online 2023 Oct 18. doi: 10.1038/s41596-023-00887-8

PMCID:: PMC10775953

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Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro

Kasey Y.C. Lau, Gianluca Amadei, Magdalena Zernicka-Goetz

Nat Protoc. Author manuscript; available in PMC 2024 Aug 1.

Published in final edited form as: Nat Protoc. 2023 Dec; 18(12): 3662–3689. Published online 2023 Oct 11. doi: 10.1038/s41596-023-00891-y

PMCID:: PMC11293611

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Efficient generation of functional neurons from mouse embryonic stem cells via neurogenin-2 expression

Yingfei Liu, Jinzhao Wang, Thomas C. Südhof, Marius Wernig

Nat Protoc. Author manuscript; available in PMC 2024 Oct 1.

Published in final edited form as: Nat Protoc. 2023 Oct; 18(10): 2954–2974. Published online 2023 Aug 18. doi: 10.1038/s41596-023-00863-2

PMCID:: PMC11349042

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Purification and functional characterization of novel human skeletal stem cell lineages

Malachia Y. Hoover, Thomas H. Ambrosi, Holly M. Steininger, Lauren S. Koepke, Yuting Wang, Liming Zhao, Matthew P. Murphy, Alina A. Alam, Elizabeth J. Arouge, M. Gohazrua K. Butler, Eri Takematsu, Suzan P. Stavitsky, Serena Hu, Debashis Sahoo, Rahul Sinha, Maurizio Morri, Norma Neff, Julius Bishop, Michael Gardner, Stuart Goodman, Michael Longaker, Charles K. F. Chan

Nat Protoc. Author manuscript; available in PMC 2023 Sep 11.

Published in final edited form as: Nat Protoc. 2023 Jul; 18(7): 2256–2282. Published online 2023 Jun 14. doi: 10.1038/s41596-023-00836-5

PMCID:: PMC10495180

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A distal lung organoid model to study interstitial lung disease, viral infection and human lung development

Ivana Matkovic Leko, Remy T. Schneider, Tania A. Thimraj, Nadine Schrode, Daniel Beitler, Hsiao-Yun Liu, Kristin Beaumont, Ya-Wen Chen, Hans-Willem Snoeck

Nat Protoc. Author manuscript; available in PMC 2024 Oct 17.

Published in final edited form as: Nat Protoc. 2023 Jul; 18(7): 2283–2312. Published online 2023 May 10. doi: 10.1038/s41596-023-00827-6

PMCID:: PMC11486529

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Click chemistry: A transformative technology in nuclear medicine

David Bauer, Samantha M. Sarrett, Jason S. Lewis, Brian M. Zeglis

Nat Protoc. Author manuscript; available in PMC 2023 Sep 1.

Published in final edited form as: Nat Protoc. 2023 Jun; 18(6): 1659–1668. Published online 2023 Apr 26. doi: 10.1038/s41596-023-00825-8

PMCID:: PMC10293801

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Large-scale F0 CRISPR Screens in vivo using MIC-Drop

Saba Parvez, Zachary J. Brandt, Randall T. Peterson

Nat Protoc. Author manuscript; available in PMC 2023 Aug 11.

Published in final edited form as: Nat Protoc. 2023 Jun; 18(6): 1841–1865. Published online 2023 Apr 17. doi: 10.1038/s41596-023-00821-y

PMCID:: PMC10419324

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Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish

Kuan-Ting Huang, Jesse R. Poganik, Saba Parvez, Sruthi Raja, Brian Miller, Marcus J. C. Long, Joseph R. Fetcho, Yimon Aye

Nat Protoc. Author manuscript; available in PMC 2024 Jun 5.

Published in final edited form as: Nat Protoc. 2023 May; 18(5): 1379–1415. Published online 2023 Apr 5. doi: 10.1038/s41596-023-00809-8

Correction in:: Nat Protoc. 2023 Jun 20

PMCID:: PMC11150335

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