Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1997 May;63(5):1696-700.
doi: 10.1128/aem.63.5.1696-1700.1997.

Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus

L Pasamontes  1 , M Haiker, M Wyss, M Tessier, A P van Loon
Affiliations

Gene cloning, purification, and characterization of a heat-stable phytase from the fungus Aspergillus fumigatus

L Pasamontes et al. Appl Environ Microbiol. 1997 May.

Abstract

The finding of heat-stable enzymes or the engineering of moderately thermostable enzymes into more stable ones by random or site-directed mutagenesis has become a main priority of modern biotechnology. We report here for the first time a heat-stable phytase able to withstand temperatures up to 100 degrees C over a period of 20 min, with a loss of only 10% of the initial enzymatic activity. The gene (phyA) encoding this heat-stable enzyme has been cloned from Aspergillus fumigatus and overexpressed in Aspergillus niger. The enzyme showed high activity with 4-nitrophenyl phosphate at a pH range of 3 to 5 and with phytic acid at a pH range of 2.5 to 7.5.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Nutr. 1971 Oct;101(10):1289-93 - PubMed
    1. Microbiology. 1997 Jan;143 ( Pt 1):245-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853-7 - PubMed
    1. Eur J Biochem. 1983 Jun 1;133(1):17-21 - PubMed

MeSH terms

Associated data

LinkOut - more resources