Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Sep 30;12(12):1187-200.
doi: 10.1002/(SICI)1097-0061(19960930)12:12%3C1187::AID-YEA986%3E3.0.CO;2-U.

Cloning of the Penicillium minioluteum gene encoding dextranase and its expression in Pichia pastoris

H Roca  1 , B Garcia, E Rodriguez, D Mateu, L Coroas, J Cremata, R Garcia, T Pons, J Delgado
Affiliations

Cloning of the Penicillium minioluteum gene encoding dextranase and its expression in Pichia pastoris

H Roca et al. Yeast. .

Abstract

The DEX gene encoding an extracellular dextranase was isolated from the genomic DNA library of Penicillium minioluteum by hybridization using the dextranase cDNA as a probe. Comparison of the gene and cDNA sequences revealed that the DEX gene does not contain introns. Amino acid sequences comparison of P. minioluteum dextranase with other reported dextranases reveals a significant homology (29% identity) with a dextranase from Arthrobacter sp. CB-8. The DEX gene fragment encoding a mature protein of 574 amino acids was expressed in the methylotrophic yeast Pichia pastoris by using the SUC2 gene signal sequence from Saccharomyces cerevisiae under control of the alcohol oxidase-1 (AOX1) promoter. Over 3.2 g/l of enzymatically active dextranase was secreted into the medium after induction by methanol. The yeast product was indistinguishable from the native enzyme in specific activity and the N-terminus of both proteins were identical.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

Publication types

MeSH terms

Associated data

LinkOut - more resources