Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Feb 15;90(4):1474-8.
doi: 10.1073/pnas.90.4.1474.

Crystal structure of Escherichia coli L-asparaginase, an enzyme used in cancer therapy

A L Swain  1 , M Jaskólski, D Housset, J K Rao, A Wlodawer
Affiliations

Crystal structure of Escherichia coli L-asparaginase, an enzyme used in cancer therapy

A L Swain et al. Proc Natl Acad Sci U S A. .

Abstract

The crystal structure of Escherichia coli asparaginase II (EC 3.5.1.1), a drug (Elspar) used for the treatment of acute lymphoblastic leukemia, has been determined at 2.3 A resolution by using data from a single heavy atom derivative in combination with molecular replacement. The atomic model was refined to an R factor of 0.143. This enzyme, active as a homotetramer with 222 symmetry, belongs to the class of alpha/beta proteins. Each subunit has two domains with unique topological features. On the basis of present structural evidence consistent with previous biochemical studies, we propose locations for the active sites between the N- and C-terminal domains belonging to different subunits and postulate a catalytic role for Thr-89.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Eur J Biochem. 1971 Jun 11;20(3):432-7 - PubMed
    1. J Enzyme Inhib. 1986;1(2):151-61 - PubMed
    1. Arch Biochem Biophys. 1972 Sep;152(1):280-6 - PubMed
    1. J Biol Chem. 1977 Mar 25;252(6):2072-6 - PubMed
    1. Arch Biochem Biophys. 1986 Jan;244(1):128-36 - PubMed

Publication types