Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Apr;27(5):435-9.
doi: 10.1007/BF00311212.

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae

Affiliations

Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae

S Sakamoto et al. Curr Genet. 1995 Apr.

Abstract

The cDNA encoding the endo-beta-1,4-glucanase (carboxymethylcellulase; CMCase-I) from Aspergillus kawachii IFO 4308 was cloned. Nucleotide-sequence analysis of the cloned cDNA insert showed a 717-bp open reading frame that encoded a protein of 239 amino-acid residues. The predicted amino-acid sequence of the mature protein had considerable homology with the protein sequence of the FI-CMCase of Aspergillus aculeatus. The cDNA was introduced into Saccharomyces cerevisiae. The expressed enzyme had carboxylmethylcellulase activity, identified by clear zones on a CMC-agar plate after Congo Red staining.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Science. 1990 Jul 27;249(4967):380-6 - PubMed
    1. Biotechnology (N Y). 1990 May;8(5):435-40 - PubMed
    1. Gene. 1989 Sep 1;81(1):83-95 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Mol Biol. 1983 Jun 5;166(4):557-80 - PubMed

Associated data

LinkOut - more resources