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Review

Analysis of Nanoparticle Effects on Invasion of Cancer Cells: Version 1.0

Julia Bui  1 , Edward Cedrone  1 , Naichen Yu  2 , Marina A. Dobrovolskaia  1
In: National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet]. Bethesda (MD): National Cancer Institute (US); 2005 May. NCL Method IEA-1.
2020 May.
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Review

Analysis of Nanoparticle Effects on Invasion of Cancer Cells: Version 1.0

Julia Bui et al.
Free Books & Documents

Excerpt

This document provides a protocol for a real-time, quantitative assessment of nanoparticle-mediated inhibition of cancer cell invasion. Although processes of cell migration and invasion have similar properties, they can be differentiated based on the ability of invasive cells to migrate through either an extracellular matrix (ECM) or a basement membrane extract (BME). This protocol uses Matrigel as an extracellular matrix and analyzes the number of cells migrating through the Matrigel and porous membrane barriers with or without prior exposure to test nanoparticles. Nanoparticles, which intended mechanism of action involves the inhibition of cancer cell invasion, will result in a decrease in the number of cells migrated through these barriers.

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References

    1. Dobrovolskaia MA, McNeil SE. Understanding the correlation between in vitro and in vivo immunotoxicity tests for nanomedicines. J Control Release. 2013;172(2):456–66. - PMC - PubMed
    1. xCELLigence® Real-Time Cell Analysis (CIM Protocol). Using the xCELLigence® RTCA DP Instrument to perform Cell Invasion and Migration (CIM) Assays, ACEA Biosciences; 2015
    1. Corning Life Sciences. Cell Migration, Chemotaxis and Invasion Assay Protocol. Corning Incorporated.

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