Laboratory testing for the diagnosis of COVID-19

doi:10.1016/j.bbrc.2020.10.069

Review

. 2021 Jan 29:538:226-230.

doi: 10.1016/j.bbrc.2020.10.069. Epub 2020 Oct 28.

Laboratory testing for the diagnosis of COVID-19

Christopher K C Lai¹, Wilson Lam²

Affiliations

¹ Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. Electronic address: chris.kclai@cuhk.edu.hk.
² Chiron Medical Group, 2/F Podium Plaza, Hanoi Road, Hong Kong Special Administrative Region, China; Department of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.

PMID: 33139015
PMCID: PMC7598306
DOI: 10.1016/j.bbrc.2020.10.069

Review

Laboratory testing for the diagnosis of COVID-19

Christopher K C Lai et al. Biochem Biophys Res Commun. 2021.

. 2021 Jan 29:538:226-230.

doi: 10.1016/j.bbrc.2020.10.069. Epub 2020 Oct 28.

Authors

Christopher K C Lai¹, Wilson Lam²

Affiliations

¹ Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. Electronic address: chris.kclai@cuhk.edu.hk.
² Chiron Medical Group, 2/F Podium Plaza, Hanoi Road, Hong Kong Special Administrative Region, China; Department of Medicine and Therapeutics, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China.

PMID: 33139015
PMCID: PMC7598306
DOI: 10.1016/j.bbrc.2020.10.069

Abstract

Rapid and accurate laboratory diagnosis of active COVID-19 infection is one of the cornerstones of pandemic control. With the myriad of tests available in the market, the use of correct specimen type and laboratory-testing technique in the right clinical scenario could be challenging for non-specialists. In this mini-review, we will discuss the difference in diagnostic performance for different upper and lower respiratory tract specimens, and the role of blood and fecal specimens. We will analyze the performance characteristics of laboratory testing techniques of nucleic acid amplification tests, antigen detection tests, antibody detection tests, and point-of-care tests. Finally, the dynamics of viral replication and antibody production, and laboratory results interpretation in conjunction with clinical scenarios will be discussed.

Keywords: COVID-19; Coronavirus; Diagnosis; SARS-CoV-2.

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References

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[1] World Health Organization . Vol. 2019. WHO - Interim Guid.; 2020. Laboratory testing for 2019 novel coronavirus (2019-nCoV) in suspected human cases; pp. 1–7.https://www.who.int/publications/i/item/laboratory-testing-of-2019-novel... January. Accessed.

[2] World Health Organization . Vol. 2019. WHO - Interim Guid.; 2020. Laboratory testing for 2019 novel coronavirus (2019-nCoV) in suspected human cases; pp. 1–7.https://www.who.int/publications/i/item/laboratory-testing-of-2019-novel... January. Accessed.

[3] Huang C., Wang Y., Li X., et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497–506. doi: 10.1016/S0140-6736(20)30183-5. - DOI - PMC - PubMed

[4] Huang C., Wang Y., Li X., et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497–506. doi: 10.1016/S0140-6736(20)30183-5. - DOI - PMC - PubMed

[5] Hanson KE, Caliendo AM, Arias CA, et al. Last updated May 6, 2020 and posted online at www.idsociety.org/COVID19guidelines/dx.Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19. www.idsociety.org/COVID19guidelines/dx..

[6] Hanson KE, Caliendo AM, Arias CA, et al. Last updated May 6, 2020 and posted online at www.idsociety.org/COVID19guidelines/dx.Infectious Diseases Society of America Guidelines on the Diagnosis of COVID-19. www.idsociety.org/COVID19guidelines/dx..

[7] World Health Organization. Diagnostic testing for SARS-CoV-2. Interim guidance 11 September 2020. https://www.who.int/publications/i/item/diagnostic-testing-for-sars-cov-2 (Accessed 30 Sep 2020).

[8] World Health Organization. Diagnostic testing for SARS-CoV-2. Interim guidance 11 September 2020. https://www.who.int/publications/i/item/diagnostic-testing-for-sars-cov-2 (Accessed 30 Sep 2020).

[9] Spencer S., Thompson M.G., Flannery B., et al. Comparison of respiratory specimen collection methods for detection of Influenza virus infection by reverse transcription-PCR: a literature review. J. Clin. Microbiol. 2019;57(9) doi: 10.1128/JCM.00027-19. - DOI - PMC - PubMed

[10] Spencer S., Thompson M.G., Flannery B., et al. Comparison of respiratory specimen collection methods for detection of Influenza virus infection by reverse transcription-PCR: a literature review. J. Clin. Microbiol. 2019;57(9) doi: 10.1128/JCM.00027-19. - DOI - PMC - PubMed

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Laboratory testing for the diagnosis of COVID-19

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Laboratory testing for the diagnosis of COVID-19

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