Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Dec;35(12):1597-604.
doi: 10.1007/s10295-008-0403-0. Epub 2008 Sep 12.

Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1

Namir I A Haddad  1 , Ji Wang, Bozhong Mu
Affiliations

Isolation and characterization of a biosurfactant producing strain, Brevibacilis brevis HOB1

Namir I A Haddad et al. J Ind Microbiol Biotechnol. 2008 Dec.

Abstract

Biosurfactant-producing bacteria were isolated from the production water of an oil field. Isolates were screened for biosurfactant production using surface tension test. The highest reduction of surface tension was achieved with a bacterial strain which was identified by 16S rRNA gene sequencing as Brevibacilis brevis HOB1. It has been investigated using different carbon and nitrogen sources. It showed that the strain was able to grow and reduce the surface tension of the broth to 29 mN/m on commercial sugar and maltose, and to 32 mN/m on glucose after 72 h of growth. The maximum amount of biosurfactant was obtained when nitrate ions were supplied as nitrogen source. Biosurfactant produced by Brevibacilis brevis HOB1 was confirmed as a lipopeptide class of biosurfactant using TLC test and mass spectra. Lipopeptide isoforms were isolated from cell-free supernatants by acid-precipitation followed by one step of chromatographic separation on solid-phase ODS C18 column. The separation was confirmed by HPLC and ESI Q-TOF MS spectroscopy. Comparing the mass data obtained and the mass numbers reported for the lipopeptide complexes from other strains, it can be concluded that the major lipopeptide product of Brevibacilis brevis HOB1 is the surfactin isoform. This lipopeptide showed strong antibacterial and antifungal activity. It is a candidate for the biocontrol of pathogens in agriculture and other industries.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Curr Med Chem. 2004 Aug;11(16):2105-34 - PubMed
    1. Nat Protoc. 2008;3(2):153-62 - PubMed
    1. Biotechnology (N Y). 1992 Jan;10(1):60-5 - PubMed
    1. Appl Microbiol Biotechnol. 2000 May;53(5):495-508 - PubMed
    1. Trends Biotechnol. 2004 Mar;22(3):142-6 - PubMed

Publication types

MeSH terms

LinkOut - more resources